---
_id: '13052'
abstract:
- lang: eng
  text: Imaging of the immunological synapse (IS) between dendritic cells (DCs) and
    T cells in suspension is hampered by suboptimal alignment of cell-cell contacts
    along the vertical imaging plane. This requires optical sectioning that often
    results in unsatisfactory resolution in time and space. Here, we present a workflow
    where DCs and T cells are confined between a layer of glass and polydimethylsiloxane
    (PDMS) that orients the cells along one, horizontal imaging plane, allowing for
    fast en-face-imaging of the DC-T cell IS.
acknowledged_ssus:
- _id: Bio
- _id: NanoFab
- _id: M-Shop
acknowledgement: 'A.L. was funded by an Erwin Schrödinger postdoctoral fellowship
  of the Austrian Science Fund (FWF, project number: J4542-B) and is an EMBO non-stipendiary
  postdoctoral fellow. This work was supported by a European Research Council grant
  ERC-CoG-72437 to M.S. We thank the Imaging & Optics facility, the Nanofabrication
  facility, and the Miba Machine Shop of ISTA for their excellent support.'
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Alexander F
  full_name: Leithner, Alexander F
  id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
  last_name: Leithner
  orcid: 0000-0002-1073-744X
- first_name: Jack
  full_name: Merrin, Jack
  id: 4515C308-F248-11E8-B48F-1D18A9856A87
  last_name: Merrin
  orcid: 0000-0001-5145-4609
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
citation:
  ama: 'Leithner AF, Merrin J, Sixt MK. En-Face Imaging of T Cell-Dendritic Cell Immunological
    Synapses. In: Baldari C, Dustin M, eds. <i>The Immune Synapse</i>. Vol 2654. MIMB.
    New York, NY: Springer Nature; 2023:137-147. doi:<a href="https://doi.org/10.1007/978-1-0716-3135-5_9">10.1007/978-1-0716-3135-5_9</a>'
  apa: 'Leithner, A. F., Merrin, J., &#38; Sixt, M. K. (2023). En-Face Imaging of
    T Cell-Dendritic Cell Immunological Synapses. In C. Baldari &#38; M. Dustin (Eds.),
    <i>The Immune Synapse</i> (Vol. 2654, pp. 137–147). New York, NY: Springer Nature.
    <a href="https://doi.org/10.1007/978-1-0716-3135-5_9">https://doi.org/10.1007/978-1-0716-3135-5_9</a>'
  chicago: 'Leithner, Alexander F, Jack Merrin, and Michael K Sixt. “En-Face Imaging
    of T Cell-Dendritic Cell Immunological Synapses.” In <i>The Immune Synapse</i>,
    edited by Cosima Baldari and Michael Dustin, 2654:137–47. MIMB. New York, NY:
    Springer Nature, 2023. <a href="https://doi.org/10.1007/978-1-0716-3135-5_9">https://doi.org/10.1007/978-1-0716-3135-5_9</a>.'
  ieee: 'A. F. Leithner, J. Merrin, and M. K. Sixt, “En-Face Imaging of T Cell-Dendritic
    Cell Immunological Synapses,” in <i>The Immune Synapse</i>, vol. 2654, C. Baldari
    and M. Dustin, Eds. New York, NY: Springer Nature, 2023, pp. 137–147.'
  ista: 'Leithner AF, Merrin J, Sixt MK. 2023.En-Face Imaging of T Cell-Dendritic
    Cell Immunological Synapses. In: The Immune Synapse. Methods in Molecular Biology,
    vol. 2654, 137–147.'
  mla: Leithner, Alexander F., et al. “En-Face Imaging of T Cell-Dendritic Cell Immunological
    Synapses.” <i>The Immune Synapse</i>, edited by Cosima Baldari and Michael Dustin,
    vol. 2654, Springer Nature, 2023, pp. 137–47, doi:<a href="https://doi.org/10.1007/978-1-0716-3135-5_9">10.1007/978-1-0716-3135-5_9</a>.
  short: A.F. Leithner, J. Merrin, M.K. Sixt, in:, C. Baldari, M. Dustin (Eds.), The
    Immune Synapse, Springer Nature, New York, NY, 2023, pp. 137–147.
date_created: 2023-05-22T08:41:48Z
date_published: 2023-04-28T00:00:00Z
date_updated: 2023-10-17T08:44:53Z
day: '28'
department:
- _id: MiSi
- _id: NanoFab
doi: 10.1007/978-1-0716-3135-5_9
ec_funded: 1
editor:
- first_name: Cosima
  full_name: Baldari, Cosima
  last_name: Baldari
- first_name: Michael
  full_name: Dustin, Michael
  last_name: Dustin
external_id:
  pmid:
  - '37106180'
intvolume: '      2654'
language:
- iso: eng
month: '04'
oa_version: None
page: 137-147
place: New York, NY
pmid: 1
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
publication: The Immune Synapse
publication_identifier:
  eisbn:
  - '9781071631355'
  eissn:
  - 1940-6029
  isbn:
  - '9781071631348'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2654
year: '2023'
...