@inbook{13052,
  abstract     = {Imaging of the immunological synapse (IS) between dendritic cells (DCs) and T cells in suspension is hampered by suboptimal alignment of cell-cell contacts along the vertical imaging plane. This requires optical sectioning that often results in unsatisfactory resolution in time and space. Here, we present a workflow where DCs and T cells are confined between a layer of glass and polydimethylsiloxane (PDMS) that orients the cells along one, horizontal imaging plane, allowing for fast en-face-imaging of the DC-T cell IS.},
  author       = {Leithner, Alexander F and Merrin, Jack and Sixt, Michael K},
  booktitle    = {The Immune Synapse},
  editor       = {Baldari, Cosima and Dustin, Michael},
  isbn         = {9781071631348},
  issn         = {1940-6029},
  pages        = {137--147},
  publisher    = {Springer Nature},
  title        = {{En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses}},
  doi          = {10.1007/978-1-0716-3135-5_9},
  volume       = {2654},
  year         = {2023},
}