---
_id: '10135'
abstract:
- lang: eng
  text: "Plants maintain the capacity to develop new organs e.g. lateral roots post-embryonically
    throughout their whole life and thereby flexibly adapt to ever-changing environmental
    conditions. Plant hormones auxin and cytokinin are the main regulators of the
    lateral root organogenesis. Additionally to their solo activities, the interaction
    between auxin and\r\ncytokinin plays crucial role in fine-tuning of lateral root
    development and growth. In particular, cytokinin modulates auxin distribution
    within the developing lateral root by affecting the endomembrane trafficking of
    auxin transporter PIN1 and promoting its vacuolar degradation (Marhavý et al.,
    2011, 2014). This effect is independent of transcription and\r\ntranslation. Therefore,
    it suggests novel, non-canonical cytokinin activity occuring possibly on the posttranslational
    level. Impact of cytokinin and other plant hormones on auxin transporters (including
    PIN1) on the posttranslational level is described in detail in the introduction
    part of this thesis in a form of a review (Semeradova et al., 2020). To gain insights
    into the molecular machinery underlying cytokinin effect on the endomembrane trafficking
    in the plant cell, in particular on the PIN1 degradation, we conducted two large
    proteomic screens: 1) Identification of cytokinin binding proteins using\r\nchemical
    proteomics. 2) Monitoring of proteomic and phosphoproteomic changes upon cytokinin
    treatment. In the first screen, we identified DYNAMIN RELATED PROTEIN 2A (DRP2A).
    We found that DRP2A plays a role in cytokinin regulated processes during the plant
    growth and that cytokinin treatment promotes destabilization of DRP2A protein.
    However, the role of DRP2A in the PIN1 degradation remains to be elucidated. In
    the second screen, we found VACUOLAR PROTEIN SORTING 9A (VPS9A). VPS9a plays crucial
    role in plant’s response to cytokin and in cytokinin mediated PIN1 degradation.
    Altogether, we identified proteins, which bind to cytokinin and proteins that
    in response to\r\ncytokinin exhibit significantly changed abundance or phosphorylation
    pattern. By combining information from these two screens, we can pave our way
    towards understanding of noncanonical cytokinin effects."
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Hana
  full_name: Semerádová, Hana
  id: 42FE702E-F248-11E8-B48F-1D18A9856A87
  last_name: Semerádová
citation:
  ama: Semerádová H. Molecular mechanisms of the cytokinin-regulated endomembrane
    trafficking to coordinate plant organogenesis. 2021. doi:<a href="https://doi.org/10.15479/at:ista:10135">10.15479/at:ista:10135</a>
  apa: Semerádová, H. (2021). <i>Molecular mechanisms of the cytokinin-regulated endomembrane
    trafficking to coordinate plant organogenesis</i>. Institute of Science and Technology
    Austria. <a href="https://doi.org/10.15479/at:ista:10135">https://doi.org/10.15479/at:ista:10135</a>
  chicago: Semerádová, Hana. “Molecular Mechanisms of the Cytokinin-Regulated Endomembrane
    Trafficking to Coordinate Plant Organogenesis.” Institute of Science and Technology
    Austria, 2021. <a href="https://doi.org/10.15479/at:ista:10135">https://doi.org/10.15479/at:ista:10135</a>.
  ieee: H. Semerádová, “Molecular mechanisms of the cytokinin-regulated endomembrane
    trafficking to coordinate plant organogenesis,” Institute of Science and Technology
    Austria, 2021.
  ista: Semerádová H. 2021. Molecular mechanisms of the cytokinin-regulated endomembrane
    trafficking to coordinate plant organogenesis. Institute of Science and Technology
    Austria.
  mla: Semerádová, Hana. <i>Molecular Mechanisms of the Cytokinin-Regulated Endomembrane
    Trafficking to Coordinate Plant Organogenesis</i>. Institute of Science and Technology
    Austria, 2021, doi:<a href="https://doi.org/10.15479/at:ista:10135">10.15479/at:ista:10135</a>.
  short: H. Semerádová, Molecular Mechanisms of the Cytokinin-Regulated Endomembrane
    Trafficking to Coordinate Plant Organogenesis, Institute of Science and Technology
    Austria, 2021.
date_created: 2021-10-13T13:42:48Z
date_published: 2021-10-13T00:00:00Z
date_updated: 2024-01-25T10:53:29Z
day: '13'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: EvBe
doi: 10.15479/at:ista:10135
file:
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  date_created: 2021-10-27T07:45:37Z
  date_updated: 2022-12-20T23:30:05Z
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  relation: source_file
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  creator: cziletti
  date_created: 2021-10-27T07:45:57Z
  date_updated: 2022-12-20T23:30:05Z
  embargo: 2022-10-28
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file_date_updated: 2022-12-20T23:30:05Z
has_accepted_license: '1'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
project:
- _id: 261821BC-B435-11E9-9278-68D0E5697425
  grant_number: '24746'
  name: Molecular mechanisms of the cytokinin regulated endomembrane trafficking to
    coordinate plant organogenesis.
publication_identifier:
  isbn:
  - 978-3-99078-014-5
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '9160'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
title: Molecular mechanisms of the cytokinin-regulated endomembrane trafficking to
  coordinate plant organogenesis
type: dissertation
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
year: '2021'
...