---
_id: '3452'
abstract:
- lang: eng
  text: In recent years, considerable progress in our understanding of the molecular
    events underlying excitatory synaptic transmission has been made. This progress
    was mainly achieved by technical advances, among them the patch-clamp technique
    in brain slices (Edwards et al., 1989), fast application of agonists (Franke et
    al., 1987), and cloning and functional expression of GluR channels of the nonNMDA
    type (e.g., Hollmann et al., 1989). A suitable model for studying excitatory postsynaptic
    currents (EPSCs) in the brain slice with patch-clamp techniques is the mossy fiber
    synapse on CA3 pyramidal cells of rat hippocampus (MF-CA3 synapse). This synapse
    is located close to the cell soma and should provide almost ideal space-clamp
    conditions. A comparison of MF-CA3 EPSCs with the currents activated by fast application
    of glutamate on membrane patches isolated from CA3 cell somata suggests that the
    concentration of glutamate in the synaptic cleft during excitatory synaptic transmission
    is high (about 1 mM) and that the transmitter remains in the synaptic cleft only
    briefly (about 1 ms). It seems likely that desensitization influences the peak
    amplitude of the EPSC in several ways. Brief pulses of glutamate cause desensitization,
    from which the glutamate receptor channels recover only slowly, and micromolar
    ambient glutamate concentrations produce desensitization at equilibrium. From
    the functional properties of recombinant GluR channels, in situ hybridization
    data, and patch-clamp experiments on different neuronal and nonneuronal cell types,
    a picture of the molecular identity of native channels emerges. In neurons of
    the hippocampus the pharmacological features of these channels were similar to
    recombinant channels assembled from subunits of the AMPA/kainate subtype which
    are strongly expressed in these cells. The native channels are characterized by
    outward rectification of the steady-state I-V and low Ca permeability, similar
    to recombinant channels containing the GluR-B subunit. This is consistent with
    the ubiquitous expression of this subunit in hippocampal neurones. In contrast,
    GluR channels from cerebellar glial cells, which uniquely in the central nervous
    system lack the expression of GluR-B subunits, show double rectification and high
    Ca permeability. The results suggest that the native functional nonNMDA glutamate
    receptor channels in the CNS are assembled form subunits of the AMPA/kainate subtype
    in a cell-specific way, with the functional properties of GluR channels in neurones
    being dominated by the GluR-B subunit.
alternative_title:
- EXS
article_processing_charge: No
author:
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: 'Jonas PM. AMPA-type glutamate receptors - nonselective cation channels mediating
    fast excitatory transmission in the CNS. In: Siemen D, ed. <i>Nonselective Cation
    Channels: Pharmacology, Physiology and Biophysics.</i> Vol 66. Birkhäuser; 1993:61-76.
    doi:<a href="https://doi.org/10.1007/978-3-0348-7327-7_4">10.1007/978-3-0348-7327-7_4</a>'
  apa: 'Jonas, P. M. (1993). AMPA-type glutamate receptors - nonselective cation channels
    mediating fast excitatory transmission in the CNS. In D. Siemen (Ed.), <i>Nonselective
    cation channels: Pharmacology, Physiology and Biophysics.</i> (Vol. 66, pp. 61–76).
    Birkhäuser. <a href="https://doi.org/10.1007/978-3-0348-7327-7_4">https://doi.org/10.1007/978-3-0348-7327-7_4</a>'
  chicago: 'Jonas, Peter M. “AMPA-Type Glutamate Receptors - Nonselective Cation Channels
    Mediating Fast Excitatory Transmission in the CNS.” In <i>Nonselective Cation
    Channels: Pharmacology, Physiology and Biophysics.</i>, edited by Detlef Siemen,
    66:61–76. Birkhäuser, 1993. <a href="https://doi.org/10.1007/978-3-0348-7327-7_4">https://doi.org/10.1007/978-3-0348-7327-7_4</a>.'
  ieee: 'P. M. Jonas, “AMPA-type glutamate receptors - nonselective cation channels
    mediating fast excitatory transmission in the CNS,” in <i>Nonselective cation
    channels: Pharmacology, Physiology and Biophysics.</i>, vol. 66, D. Siemen, Ed.
    Birkhäuser, 1993, pp. 61–76.'
  ista: 'Jonas PM. 1993.AMPA-type glutamate receptors - nonselective cation channels
    mediating fast excitatory transmission in the CNS. In: Nonselective cation channels:
    Pharmacology, Physiology and Biophysics. EXS, vol. 66, 61–76.'
  mla: 'Jonas, Peter M. “AMPA-Type Glutamate Receptors - Nonselective Cation Channels
    Mediating Fast Excitatory Transmission in the CNS.” <i>Nonselective Cation Channels:
    Pharmacology, Physiology and Biophysics.</i>, edited by Detlef Siemen, vol. 66,
    Birkhäuser, 1993, pp. 61–76, doi:<a href="https://doi.org/10.1007/978-3-0348-7327-7_4">10.1007/978-3-0348-7327-7_4</a>.'
  short: 'P.M. Jonas, in:, D. Siemen (Ed.), Nonselective Cation Channels: Pharmacology,
    Physiology and Biophysics., Birkhäuser, 1993, pp. 61–76.'
date_created: 2018-12-11T12:03:24Z
date_published: 1993-01-01T00:00:00Z
date_updated: 2022-03-30T11:46:44Z
day: '01'
doi: 10.1007/978-3-0348-7327-7_4
editor:
- first_name: Detlef
  full_name: Siemen, Detlef
  last_name: Siemen
extern: '1'
external_id:
  pmid:
  - '7505664'
intvolume: '        66'
language:
- iso: eng
main_file_link:
- url: https://link.springer.com/chapter/10.1007/978-3-0348-7327-7_4
month: '01'
oa_version: None
page: 61 - 76
pmid: 1
publication: 'Nonselective cation channels: Pharmacology, Physiology and Biophysics.'
publication_identifier:
  isbn:
  - 978-3-0348-7327-7
publication_status: published
publisher: Birkhäuser
publist_id: '2935'
quality_controlled: '1'
scopus_import: '1'
status: public
title: AMPA-type glutamate receptors - nonselective cation channels mediating fast
  excitatory transmission in the CNS
type: book_chapter
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 66
year: '1993'
...