---
_id: '10268'
abstract:
- lang: eng
  text: The analysis of dynamic cellular processes such as plant cytokinesis stands
    and falls with live-cell time-lapse confocal imaging. Conventional approaches
    to time-lapse imaging of cell division in Arabidopsis root tips are tedious and
    have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous
    imaging of multiple root tips on a vertical-stage confocal microscope with automated
    root tracking. We also provide modifications of the basic protocol to implement
    this imaging method in the analysis of genetic, pharmacological or laser ablation
    wounding-mediated experimental manipulations. Our method dramatically improves
    the efficiency of cell division time-lapse imaging by increasing the throughput,
    while reducing the person-hour requirements of such experiments.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank B. De Rybel for allowing M.G. to work on this manuscript
  during a postdoc in his laboratory, and EMBO for supporting M.G. with a Long-Term
  fellowship (ALTF 1005-2019) during this time. We acknowledge the service and support
  by the Bioimaging Facility at IST Austria, and finally, we thank A. Mally for proofreading
  and correcting the manuscript.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Lukas
  full_name: Hörmayer, Lukas
  id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
  last_name: Hörmayer
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
citation:
  ama: 'Hörmayer L, Friml J, Glanc M. Automated time-lapse imaging and manipulation
    of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
    In: <i>Plant Cell Division</i>. Vol 2382. MIMB. Humana Press; 2021:105-114. doi:<a
    href="https://doi.org/10.1007/978-1-0716-1744-1_6">10.1007/978-1-0716-1744-1_6</a>'
  apa: Hörmayer, L., Friml, J., &#38; Glanc, M. (2021). Automated time-lapse imaging
    and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal
    microscopy. In <i>Plant Cell Division</i> (Vol. 2382, pp. 105–114). Humana Press.
    <a href="https://doi.org/10.1007/978-1-0716-1744-1_6">https://doi.org/10.1007/978-1-0716-1744-1_6</a>
  chicago: Hörmayer, Lukas, Jiří Friml, and Matous Glanc. “Automated Time-Lapse Imaging
    and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal
    Microscopy.” In <i>Plant Cell Division</i>, 2382:105–14. MIMB. Humana Press, 2021.
    <a href="https://doi.org/10.1007/978-1-0716-1744-1_6">https://doi.org/10.1007/978-1-0716-1744-1_6</a>.
  ieee: L. Hörmayer, J. Friml, and M. Glanc, “Automated time-lapse imaging and manipulation
    of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy,”
    in <i>Plant Cell Division</i>, vol. 2382, Humana Press, 2021, pp. 105–114.
  ista: 'Hörmayer L, Friml J, Glanc M. 2021.Automated time-lapse imaging and manipulation
    of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
    In: Plant Cell Division. Methods in Molecular Biology, vol. 2382, 105–114.'
  mla: Hörmayer, Lukas, et al. “Automated Time-Lapse Imaging and Manipulation of Cell
    Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” <i>Plant
    Cell Division</i>, vol. 2382, Humana Press, 2021, pp. 105–14, doi:<a href="https://doi.org/10.1007/978-1-0716-1744-1_6">10.1007/978-1-0716-1744-1_6</a>.
  short: L. Hörmayer, J. Friml, M. Glanc, in:, Plant Cell Division, Humana Press,
    2021, pp. 105–114.
date_created: 2021-11-11T10:03:30Z
date_published: 2021-10-28T00:00:00Z
date_updated: 2022-06-03T06:47:06Z
day: '28'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-1744-1_6
external_id:
  pmid:
  - '34705235'
intvolume: '      2382'
language:
- iso: eng
month: '10'
oa_version: None
page: 105-114
pmid: 1
publication: Plant Cell Division
publication_identifier:
  eisbn:
  - 978-1-0716-1744-1
  eissn:
  - 1940-6029
  isbn:
  - 978-1-0716-1743-4
  issn:
  - 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis
  roots by vertical-stage confocal microscopy
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2382
year: '2021'
...