[{"date_created":"2018-12-11T12:03:25Z","type":"book_chapter","_id":"3454","date_updated":"2022-06-28T09:13:01Z","publisher":"Plenum","title":"Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices","oa_version":"None","editor":[{"last_name":"Sakmann","full_name":"Sakmann, Bert","first_name":"Bert"},{"full_name":"Neher, Erwin","last_name":"Neher","first_name":"Erwin"}],"day":"01","article_processing_charge":"No","doi":"10.1007/978-1-4419-1229-9_16","author":[{"first_name":"Hannah","last_name":"Monyer","full_name":"Monyer, Hannah"},{"first_name":"Peter M","orcid":"0000-0001-5001-4804","id":"353C1B58-F248-11E8-B48F-1D18A9856A87","full_name":"Jonas, Peter M","last_name":"Jonas"}],"publication_status":"published","publication_identifier":{"isbn":["978-0-306-44870-6"]},"quality_controlled":"1","main_file_link":[{"url":"https://link.springer.com/chapter/10.1007/978-1-4419-1229-9_16"}],"abstract":[{"lang":"eng","text":"The study of gene expression and regulation in the central nervous system (CNS) is a daunting task because of the diversity of neuronal phenotypes and the complexity of many protein classes. Molecular cloning revealed the presence of a large number of different protein families in the CNS, each comprising several members. Ligand-gated ion channels may serve as an example to illustrate this point (for review, see Unwin, 1993). Heterologous expression combined with electrophysiological analysis suggests that ligand-gated channels are multimeric proteins with functional properties depending on the subunit composition. Very little is known, however, about how the functional properties of the recombinant and native receptors relate to each other. Thus, it is of eminent importance to elucidate the subunit expression profile in different types of neurons in the CNS and to correlate this with the functional properties of the native receptors."}],"page":"357 - 373","publist_id":"2933","month":"01","year":"1995","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"1995-01-01T00:00:00Z","citation":{"chicago":"Monyer, Hannah, and Peter M Jonas. “Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.” In <i>Single-Channel Recording</i>, edited by Bert Sakmann and Erwin Neher, 357–73. Plenum, 1995. <a href=\"https://doi.org/10.1007/978-1-4419-1229-9_16\">https://doi.org/10.1007/978-1-4419-1229-9_16</a>.","ista":"Monyer H, Jonas PM. 1995.Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices. In: Single-channel recording. , 357–373.","mla":"Monyer, Hannah, and Peter M. Jonas. “Polymerase Chain Reaction Analysis of Ion Channel Expression in Single Neurons of Brain Slices.” <i>Single-Channel Recording</i>, edited by Bert Sakmann and Erwin Neher, Plenum, 1995, pp. 357–73, doi:<a href=\"https://doi.org/10.1007/978-1-4419-1229-9_16\">10.1007/978-1-4419-1229-9_16</a>.","apa":"Monyer, H., &#38; Jonas, P. M. (1995). Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices. In B. Sakmann &#38; E. Neher (Eds.), <i>Single-channel recording</i> (pp. 357–373). Plenum. <a href=\"https://doi.org/10.1007/978-1-4419-1229-9_16\">https://doi.org/10.1007/978-1-4419-1229-9_16</a>","ama":"Monyer H, Jonas PM. Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices. In: Sakmann B, Neher E, eds. <i>Single-Channel Recording</i>. Plenum; 1995:357-373. doi:<a href=\"https://doi.org/10.1007/978-1-4419-1229-9_16\">10.1007/978-1-4419-1229-9_16</a>","short":"H. Monyer, P.M. Jonas, in:, B. Sakmann, E. Neher (Eds.), Single-Channel Recording, Plenum, 1995, pp. 357–373.","ieee":"H. Monyer and P. M. Jonas, “Polymerase chain reaction analysis of ion channel expression in single neurons of brain slices,” in <i>Single-channel recording</i>, B. Sakmann and E. Neher, Eds. Plenum, 1995, pp. 357–373."},"language":[{"iso":"eng"}],"publication":"Single-channel recording","extern":"1","status":"public"},{"date_published":"1995-01-01T00:00:00Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","citation":{"apa":"Jonas, P. M. (1995). Fast application of agonists to isolated membrane patches. In B. Sakmann &#38; E. Neher (Eds.), <i>Single-channel recording</i> (pp. 231–243). Plenum. <a href=\"https://doi.org/10.1007/978-1-4419-1229-9_10\">https://doi.org/10.1007/978-1-4419-1229-9_10</a>","mla":"Jonas, Peter M. “Fast Application of Agonists to Isolated Membrane Patches.” <i>Single-Channel Recording</i>, edited by Bert Sakmann and Erwin Neher, Plenum, 1995, pp. 231–43, doi:<a href=\"https://doi.org/10.1007/978-1-4419-1229-9_10\">10.1007/978-1-4419-1229-9_10</a>.","chicago":"Jonas, Peter M. “Fast Application of Agonists to Isolated Membrane Patches.” In <i>Single-Channel Recording</i>, edited by Bert Sakmann and Erwin Neher, 231–43. Plenum, 1995. <a href=\"https://doi.org/10.1007/978-1-4419-1229-9_10\">https://doi.org/10.1007/978-1-4419-1229-9_10</a>.","ista":"Jonas PM. 1995.Fast application of agonists to isolated membrane patches. In: Single-channel recording. , 231–243.","ieee":"P. M. Jonas, “Fast application of agonists to isolated membrane patches,” in <i>Single-channel recording</i>, B. Sakmann and E. Neher, Eds. Plenum, 1995, pp. 231–243.","short":"P.M. Jonas, in:, B. Sakmann, E. Neher (Eds.), Single-Channel Recording, Plenum, 1995, pp. 231–243.","ama":"Jonas PM. Fast application of agonists to isolated membrane patches. In: Sakmann B, Neher E, eds. <i>Single-Channel Recording</i>. Plenum; 1995:231-243. doi:<a href=\"https://doi.org/10.1007/978-1-4419-1229-9_10\">10.1007/978-1-4419-1229-9_10</a>"},"status":"public","language":[{"iso":"eng"}],"extern":"1","publication":"Single-channel recording","publist_id":"2932","month":"01","year":"1995","quality_controlled":"1","publication_identifier":{"isbn":["978-0-306-44870-6"]},"publication_status":"published","main_file_link":[{"url":"https://link.springer.com/chapter/10.1007/978-1-4419-1229-9_10"}],"abstract":[{"text":"At a synapse, the transmitter is stored in synaptic vesicles and is released into the synaptic cleft almost instantaneously upon fusion of these vesicles with the presynaptic membrane. Subsequently, the transmitter diffuses to ligand-gated ion channels in the postsynaptic density, binds to them, and thereby causes channel activation. Unfortunately, we have estimates neither of the exact amount of transmitter in the synaptic vesicle nor of the concentration in the synaptic cleft reaching the postsynaptic receptors, and in some cases even the identity of the transmitter is unknown. These questions may be addressed by modeling of release and diffusion. Such a theoretical approach, however, is based on several assumptions, some of which lack experimental evidence.","lang":"eng"}],"page":"231 - 243","type":"book_chapter","date_created":"2018-12-11T12:03:25Z","date_updated":"2022-06-28T08:51:40Z","_id":"3455","editor":[{"full_name":"Sakmann, Bert","last_name":"Sakmann","first_name":"Bert"},{"last_name":"Neher","full_name":"Neher, Erwin","first_name":"Erwin"}],"title":"Fast application of agonists to isolated membrane patches","publisher":"Plenum","oa_version":"None","author":[{"first_name":"Peter M","orcid":"0000-0001-5001-4804","last_name":"Jonas","full_name":"Jonas, Peter M","id":"353C1B58-F248-11E8-B48F-1D18A9856A87"}],"doi":"10.1007/978-1-4419-1229-9_10","day":"01","article_processing_charge":"No"}]