@article{14794,
  abstract     = {Mosaic analysis with double markers (MADM) technology enables the sparse labeling of genetically defined neurons. We present a protocol for time-lapse imaging of cortical projection neuron migration in mice using MADM. We describe steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled brain tissue. While this protocol is compatible with other single-cell labeling methods, the MADM approach provides a genetic platform for the functional assessment of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous effects.

For complete details on the use and execution of this protocol, please refer to Hansen et al. (2022),1 Contreras et al. (2021),2 and Amberg and Hippenmeyer (2021).3},
  author       = {Hansen, Andi H and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {1},
  publisher    = {Elsevier},
  title        = {{Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers}},
  doi          = {10.1016/j.xpro.2023.102795},
  volume       = {5},
  year         = {2024},
}

@article{10321,
  abstract     = {Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice. MADM enables concomitant fluorescent cell labeling and introduction of a mutation of a gene of interest with single-cell resolution. This protocol highlights major steps for the generation of genetic mosaic tissue and the isolation and processing of respective tissues for downstream histological analysis. For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).},
  author       = {Amberg, Nicole and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {4},
  publisher    = {Cell Press},
  title        = {{Genetic mosaic dissection of candidate genes in mice using mosaic analysis with double markers}},
  doi          = {10.1016/j.xpro.2021.100939},
  volume       = {2},
  year         = {2021},
}

@article{10565,
  abstract     = {Enzymatic digestion of the extracellular matrix with chondroitinase-ABC reinstates juvenile-like plasticity in the adult cortex as it also disassembles the perineuronal nets (PNNs). The disadvantage of the enzyme is that it must be applied intracerebrally and it degrades the ECM for several weeks. Here, we provide two minimally invasive and transient protocols for microglia-enabled PNN disassembly in mouse cortex: repeated treatment with ketamine-xylazine-acepromazine (KXA) anesthesia and 60-Hz light entrainment. We also discuss how to analyze PNNs within microglial endosomes-lysosomes. For complete details on the use and execution of this protocol, please refer to Venturino et al. (2021).},
  author       = {Venturino, Alessandro and Siegert, Sandra},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {4},
  publisher    = {Elsevier ; Cell Press},
  title        = {{Minimally invasive protocols and quantification for microglia-mediated perineuronal net disassembly in mouse brain}},
  doi          = {10.1016/j.xpro.2021.101012},
  volume       = {2},
  year         = {2021},
}