---
_id: '13209'
abstract:
- lang: eng
  text: The phytohormone auxin plays central roles in many growth and developmental
    processes in plants. Development of chemical tools targeting the auxin pathway
    is useful for both plant biology and agriculture. Here we reveal that naproxen,
    a synthetic compound with anti-inflammatory activity in humans, acts as an auxin
    transport inhibitor targeting PIN-FORMED (PIN) transporters in plants. Physiological
    experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated
    developmental processes. Additional cellular and biochemical evidence indicates
    that naproxen suppresses auxin transport, specifically PIN-mediated auxin efflux.
    Moreover, biochemical and structural analyses confirm that naproxen binds directly
    to PIN1 protein via the same binding cavity as the indole-3-acetic acid substrate.
    Thus, by combining cellular, biochemical, and structural approaches, this study
    clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying
    mechanisms. Further use of this compound may advance our understanding of the
    molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in
    auxin biology and agriculture.
acknowledgement: "This work was supported by the Strategic Priority Research Program
  of the Chinese Academy of Sciences (XDB37020103 to Linfeng Sun); research funds
  from the Center for Advanced Interdisciplinary Science\r\nand Biomedicine of IHM,
  Division of Life Sciences and Medicine, University of Science and Technology of
  China (QYPY20220012 to S.T.); start-up funding from the University of Science and
  Technology of China and the\r\nChinese Academy of Sciences (GG9100007007, KY9100000026,KY9100000051,
  and KJ2070000079 to S.T.); the National Natural Science Foundation of China (31900885
  to X.L. and 31870732 to Linfeng Sun); the Natural Science Foundation of Anhui Province
  (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun); the Fundamental Research Funds
  for the Central Universities (WK9100000021 to S.T. and WK9100000031 to Linfeng Sun);
  and the USTC Research Funds of the Double First-Class Initiative (YD9100002016 to
  S.T. and YD9100002004 to Linfeng Sun). Linfeng Sun is supported by an Outstanding
  Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young
  Scholar Award from the Cyrus Tang Foundation.We thank Dr. Yang Zhao for sharing
  published materials (Center for Excellence in Molecular Plant Sciences, Chinese
  Academy of Sciences) and the Cryo-EM Center of the University of Science and Technology
  of China for the EM facility support. We are grateful to Y. Gao and all other staff
  members for their technical support on cryo-EM data collection. "
article_number: '100632'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jing
  full_name: Xia, Jing
  last_name: Xia
- first_name: Mengjuan
  full_name: Kong, Mengjuan
  last_name: Kong
- first_name: Zhisen
  full_name: Yang, Zhisen
  last_name: Yang
- first_name: Lianghanxiao
  full_name: Sun, Lianghanxiao
  last_name: Sun
- first_name: Yakun
  full_name: Peng, Yakun
  last_name: Peng
- first_name: Yanbo
  full_name: Mao, Yanbo
  last_name: Mao
- first_name: Hong
  full_name: Wei, Hong
  last_name: Wei
- first_name: Wei
  full_name: Ying, Wei
  last_name: Ying
- first_name: Yongxiao
  full_name: Gao, Yongxiao
  last_name: Gao
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Jianping
  full_name: Weng, Jianping
  last_name: Weng
- first_name: Xin
  full_name: Liu, Xin
  last_name: Liu
- first_name: Linfeng
  full_name: Sun, Linfeng
  last_name: Sun
- first_name: Shutang
  full_name: Tan, Shutang
  last_name: Tan
citation:
  ama: Xia J, Kong M, Yang Z, et al. Chemical inhibition of Arabidopsis PIN-FORMED
    auxin transporters by the anti-inflammatory drug naproxen. <i>Plant Communications</i>.
    2023;4(6). doi:<a href="https://doi.org/10.1016/j.xplc.2023.100632">10.1016/j.xplc.2023.100632</a>
  apa: Xia, J., Kong, M., Yang, Z., Sun, L., Peng, Y., Mao, Y., … Tan, S. (2023).
    Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
    drug naproxen. <i>Plant Communications</i>. Elsevier . <a href="https://doi.org/10.1016/j.xplc.2023.100632">https://doi.org/10.1016/j.xplc.2023.100632</a>
  chicago: Xia, Jing, Mengjuan Kong, Zhisen Yang, Lianghanxiao Sun, Yakun Peng, Yanbo
    Mao, Hong Wei, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
    by the Anti-Inflammatory Drug Naproxen.” <i>Plant Communications</i>. Elsevier
    , 2023. <a href="https://doi.org/10.1016/j.xplc.2023.100632">https://doi.org/10.1016/j.xplc.2023.100632</a>.
  ieee: J. Xia <i>et al.</i>, “Chemical inhibition of Arabidopsis PIN-FORMED auxin
    transporters by the anti-inflammatory drug naproxen,” <i>Plant Communications</i>,
    vol. 4, no. 6. Elsevier , 2023.
  ista: Xia J, Kong M, Yang Z, Sun L, Peng Y, Mao Y, Wei H, Ying W, Gao Y, Friml J,
    Weng J, Liu X, Sun L, Tan S. 2023. Chemical inhibition of Arabidopsis PIN-FORMED
    auxin transporters by the anti-inflammatory drug naproxen. Plant Communications.
    4(6), 100632.
  mla: Xia, Jing, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
    by the Anti-Inflammatory Drug Naproxen.” <i>Plant Communications</i>, vol. 4,
    no. 6, 100632, Elsevier , 2023, doi:<a href="https://doi.org/10.1016/j.xplc.2023.100632">10.1016/j.xplc.2023.100632</a>.
  short: J. Xia, M. Kong, Z. Yang, L. Sun, Y. Peng, Y. Mao, H. Wei, W. Ying, Y. Gao,
    J. Friml, J. Weng, X. Liu, L. Sun, S. Tan, Plant Communications 4 (2023).
date_created: 2023-07-12T07:32:00Z
date_published: 2023-11-13T00:00:00Z
date_updated: 2024-01-30T10:55:34Z
day: '13'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100632
external_id:
  isi:
  - '001113003000001'
  pmid:
  - '37254481'
file:
- access_level: open_access
  checksum: f8ef92af6096834f91ce38587fb1db9f
  content_type: application/pdf
  creator: dernst
  date_created: 2024-01-30T10:54:40Z
  date_updated: 2024-01-30T10:54:40Z
  file_id: '14900'
  file_name: 2023_PlantCommunications_Xia.pdf
  file_size: 1434862
  relation: main_file
  success: 1
file_date_updated: 2024-01-30T10:54:40Z
has_accepted_license: '1'
intvolume: '         4'
isi: 1
issue: '6'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Communications
publication_identifier:
  eissn:
  - 2590-3462
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
status: public
title: Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
  drug naproxen
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2023'
...