---
_id: '14041'
abstract:
- lang: eng
  text: Tissue morphogenesis and patterning during development involve the segregation
    of cell types. Segregation is driven by differential tissue surface tensions generated
    by cell types through controlling cell-cell contact formation by regulating adhesion
    and actomyosin contractility-based cellular cortical tensions. We use vertebrate
    tissue cell types and zebrafish germ layer progenitors as in vitro models of 3-dimensional
    heterotypic segregation and developed a quantitative analysis of their dynamics
    based on 3D time-lapse microscopy. We show that general inhibition of actomyosin
    contractility by the Rho kinase inhibitor Y27632 delays segregation. Cell type-specific
    inhibition of non-muscle myosin2 activity by overexpression of myosin assembly
    inhibitor S100A4 reduces tissue surface tension, manifested in decreased compaction
    during aggregation and inverted geometry observed during segregation. The same
    is observed when we express a constitutively active Rho kinase isoform to ubiquitously
    keep actomyosin contractility high at cell-cell and cell-medium interfaces and
    thus overriding the interface-specific regulation of cortical tensions. Tissue
    surface tension regulation can become an effective tool in tissue engineering.
acknowledgement: "We thank Marton Gulyas (ELTE Eötvös University) for development
  of videomicroscopy experiment manager and image analysis software. Authors are grateful
  to Gabor Forgacs (University of Missouri) for critical reading of earlier versions
  of this manuscript as well as to Zsuzsa Akos and Andras Czirok (ELTE Eötvös University)
  for fruitful discussions. This work was supported by EU FP7, ERC COLLMOT Project
  No 227878 to TV, the National Research Development and Innovation Fund of Hungary,
  K119359 and also Project No 2018-1.2.1-NKP-2018-00005 to LN. This project has received
  funding from the European Union’s Horizon 2020 research and innovation programme
  under the Marie Sklodowska-Curie grant agreement No 955576. MV was supported by
  the Ja´nos Bolyai Fellowship of the Hungarian Academy of Sciences.\r\nOpen access
  funding provided by Eötvös Loránd University."
article_number: '817'
article_processing_charge: Yes
article_type: original
author:
- first_name: Elod
  full_name: Méhes, Elod
  last_name: Méhes
- first_name: Enys
  full_name: Mones, Enys
  last_name: Mones
- first_name: Máté
  full_name: Varga, Máté
  last_name: Varga
- first_name: Áron
  full_name: Zsigmond, Áron
  last_name: Zsigmond
- first_name: Beáta
  full_name: Biri-Kovács, Beáta
  last_name: Biri-Kovács
- first_name: László
  full_name: Nyitray, László
  last_name: Nyitray
- first_name: Vanessa
  full_name: Barone, Vanessa
  id: 419EECCC-F248-11E8-B48F-1D18A9856A87
  last_name: Barone
  orcid: 0000-0003-2676-3367
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Tamás
  full_name: Vicsek, Tamás
  last_name: Vicsek
citation:
  ama: Méhes E, Mones E, Varga M, et al. 3D cell segregation geometry and dynamics
    are governed by tissue surface tension regulation. <i>Communications Biology</i>.
    2023;6. doi:<a href="https://doi.org/10.1038/s42003-023-05181-7">10.1038/s42003-023-05181-7</a>
  apa: Méhes, E., Mones, E., Varga, M., Zsigmond, Á., Biri-Kovács, B., Nyitray, L.,
    … Vicsek, T. (2023). 3D cell segregation geometry and dynamics are governed by
    tissue surface tension regulation. <i>Communications Biology</i>. Springer Nature.
    <a href="https://doi.org/10.1038/s42003-023-05181-7">https://doi.org/10.1038/s42003-023-05181-7</a>
  chicago: Méhes, Elod, Enys Mones, Máté Varga, Áron Zsigmond, Beáta Biri-Kovács,
    László Nyitray, Vanessa Barone, Gabriel Krens, Carl-Philipp J Heisenberg, and
    Tamás Vicsek. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue
    Surface Tension Regulation.” <i>Communications Biology</i>. Springer Nature, 2023.
    <a href="https://doi.org/10.1038/s42003-023-05181-7">https://doi.org/10.1038/s42003-023-05181-7</a>.
  ieee: E. Méhes <i>et al.</i>, “3D cell segregation geometry and dynamics are governed
    by tissue surface tension regulation,” <i>Communications Biology</i>, vol. 6.
    Springer Nature, 2023.
  ista: Méhes E, Mones E, Varga M, Zsigmond Á, Biri-Kovács B, Nyitray L, Barone V,
    Krens G, Heisenberg C-PJ, Vicsek T. 2023. 3D cell segregation geometry and dynamics
    are governed by tissue surface tension regulation. Communications Biology. 6,
    817.
  mla: Méhes, Elod, et al. “3D Cell Segregation Geometry and Dynamics Are Governed
    by Tissue Surface Tension Regulation.” <i>Communications Biology</i>, vol. 6,
    817, Springer Nature, 2023, doi:<a href="https://doi.org/10.1038/s42003-023-05181-7">10.1038/s42003-023-05181-7</a>.
  short: E. Méhes, E. Mones, M. Varga, Á. Zsigmond, B. Biri-Kovács, L. Nyitray, V.
    Barone, G. Krens, C.-P.J. Heisenberg, T. Vicsek, Communications Biology 6 (2023).
date_created: 2023-08-13T22:01:13Z
date_published: 2023-08-04T00:00:00Z
date_updated: 2023-12-13T12:07:33Z
day: '04'
ddc:
- '570'
department:
- _id: CaHe
- _id: Bio
doi: 10.1038/s42003-023-05181-7
external_id:
  isi:
  - '001042544100001'
  pmid:
  - '37542157'
file:
- access_level: open_access
  checksum: 1f9324f736bdbb76426b07736651c4cd
  content_type: application/pdf
  creator: dernst
  date_created: 2023-08-14T07:17:36Z
  date_updated: 2023-08-14T07:17:36Z
  file_id: '14045'
  file_name: 2023_CommBiology_Mehes.pdf
  file_size: 10181997
  relation: main_file
  success: 1
file_date_updated: 2023-08-14T07:17:36Z
has_accepted_license: '1'
intvolume: '         6'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: Communications Biology
publication_identifier:
  eissn:
  - 2399-3642
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: 3D cell segregation geometry and dynamics are governed by tissue surface tension
  regulation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 6
year: '2023'
...
---
_id: '11339'
abstract:
- lang: eng
  text: The interaction between a cell and its environment shapes fundamental intracellular
    processes such as cellular metabolism. In most cases growth rate is treated as
    a proximal metric for understanding the cellular metabolic status. However, changes
    in growth rate might not reflect metabolic variations in individuals responding
    to environmental fluctuations. Here we use single-cell microfluidics-microscopy
    combined with transcriptomics, proteomics and mathematical modelling to quantify
    the accumulation of glucose within Escherichia coli cells. In contrast to the
    current consensus, we reveal that environmental conditions which are comparatively
    unfavourable for growth, where both nutrients and salinity are depleted, increase
    glucose accumulation rates in individual bacteria and population subsets. We find
    that these changes in metabolic function are underpinned by variations at the
    translational and posttranslational level but not at the transcriptional level
    and are not dictated by changes in cell size. The metabolic response-characteristics
    identified greatly advance our fundamental understanding of the interactions between
    bacteria and their environment and have important ramifications when investigating
    cellular processes where salinity plays an important role.
acknowledgement: G.G. was supported by an EPSRC DTP PhD studentship (EP/M506527/1).
  M.V. and K.T.A. gratefully acknowledge financial support from the EPSRC (EP/N014391/1).
  U.L. was supported through a BBSRC grant (BB/V008021/1) and an MRC Proximity to
  Discovery EXCITEME2 grant (MCPC17189). This work was further supported by a Royal
  Society Research Grant (RG180007) awarded to S.P. and a QUEX Initiator grant awarded
  to S.P. and K.T.A.. D.S.M., T.A.R. and S.P.’s work in this area is also supported
  by a Marie Skłodowska-Curie project SINGEK (H2020-MSCA-ITN-2015-675752) and the
  Gordon and Betty Moore Foundation Marine Microbiology Initiative (GBMF5514). B.M.I.
  acknowledges support from a Wellcome Trust Institutional Strategic Support Award
  to the University of Exeter (204909/Z/16/Z). This project utilised equipment funded
  by the Wellcome Trust Institutional Strategic Support Fund (WT097835MF), Wellcome
  Trust Multi User Equipment Award (WT101650MA) and BBSRC LOLA award (BB/K003240/1).
article_number: '385'
article_processing_charge: No
article_type: original
author:
- first_name: Georgina
  full_name: Glover, Georgina
  last_name: Glover
- first_name: Margaritis
  full_name: Voliotis, Margaritis
  last_name: Voliotis
- first_name: Urszula
  full_name: Łapińska, Urszula
  last_name: Łapińska
- first_name: Brandon M.
  full_name: Invergo, Brandon M.
  last_name: Invergo
- first_name: Darren
  full_name: Soanes, Darren
  last_name: Soanes
- first_name: Paul
  full_name: O’Neill, Paul
  last_name: O’Neill
- first_name: Karen
  full_name: Moore, Karen
  last_name: Moore
- first_name: Nela
  full_name: Nikolic, Nela
  id: 42D9CABC-F248-11E8-B48F-1D18A9856A87
  last_name: Nikolic
  orcid: 0000-0001-9068-6090
- first_name: Peter
  full_name: Petrov, Peter
  last_name: Petrov
- first_name: David S.
  full_name: Milner, David S.
  last_name: Milner
- first_name: Sumita
  full_name: Roy, Sumita
  last_name: Roy
- first_name: Kate
  full_name: Heesom, Kate
  last_name: Heesom
- first_name: Thomas A.
  full_name: Richards, Thomas A.
  last_name: Richards
- first_name: Krasimira
  full_name: Tsaneva-Atanasova, Krasimira
  last_name: Tsaneva-Atanasova
- first_name: Stefano
  full_name: Pagliara, Stefano
  last_name: Pagliara
citation:
  ama: Glover G, Voliotis M, Łapińska U, et al. Nutrient and salt depletion synergistically
    boosts glucose metabolism in individual Escherichia coli cells. <i>Communications
    Biology</i>. 2022;5. doi:<a href="https://doi.org/10.1038/s42003-022-03336-6">10.1038/s42003-022-03336-6</a>
  apa: Glover, G., Voliotis, M., Łapińska, U., Invergo, B. M., Soanes, D., O’Neill,
    P., … Pagliara, S. (2022). Nutrient and salt depletion synergistically boosts
    glucose metabolism in individual Escherichia coli cells. <i>Communications Biology</i>.
    Springer Nature. <a href="https://doi.org/10.1038/s42003-022-03336-6">https://doi.org/10.1038/s42003-022-03336-6</a>
  chicago: Glover, Georgina, Margaritis Voliotis, Urszula Łapińska, Brandon M. Invergo,
    Darren Soanes, Paul O’Neill, Karen Moore, et al. “Nutrient and Salt Depletion
    Synergistically Boosts Glucose Metabolism in Individual Escherichia Coli Cells.”
    <i>Communications Biology</i>. Springer Nature, 2022. <a href="https://doi.org/10.1038/s42003-022-03336-6">https://doi.org/10.1038/s42003-022-03336-6</a>.
  ieee: G. Glover <i>et al.</i>, “Nutrient and salt depletion synergistically boosts
    glucose metabolism in individual Escherichia coli cells,” <i>Communications Biology</i>,
    vol. 5. Springer Nature, 2022.
  ista: Glover G, Voliotis M, Łapińska U, Invergo BM, Soanes D, O’Neill P, Moore K,
    Nikolic N, Petrov P, Milner DS, Roy S, Heesom K, Richards TA, Tsaneva-Atanasova
    K, Pagliara S. 2022. Nutrient and salt depletion synergistically boosts glucose
    metabolism in individual Escherichia coli cells. Communications Biology. 5, 385.
  mla: Glover, Georgina, et al. “Nutrient and Salt Depletion Synergistically Boosts
    Glucose Metabolism in Individual Escherichia Coli Cells.” <i>Communications Biology</i>,
    vol. 5, 385, Springer Nature, 2022, doi:<a href="https://doi.org/10.1038/s42003-022-03336-6">10.1038/s42003-022-03336-6</a>.
  short: G. Glover, M. Voliotis, U. Łapińska, B.M. Invergo, D. Soanes, P. O’Neill,
    K. Moore, N. Nikolic, P. Petrov, D.S. Milner, S. Roy, K. Heesom, T.A. Richards,
    K. Tsaneva-Atanasova, S. Pagliara, Communications Biology 5 (2022).
date_created: 2022-05-01T22:01:41Z
date_published: 2022-04-20T00:00:00Z
date_updated: 2023-08-03T06:45:26Z
day: '20'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.1038/s42003-022-03336-6
external_id:
  isi:
  - '000784143400001'
  pmid:
  - '35444215'
file:
- access_level: open_access
  checksum: 7c6f76ab17393d650825cc240edc84b3
  content_type: application/pdf
  creator: dernst
  date_created: 2022-05-02T06:26:26Z
  date_updated: 2022-05-02T06:26:26Z
  file_id: '11342'
  file_name: 2022_CommBiology_Glover.pdf
  file_size: 2827723
  relation: main_file
  success: 1
file_date_updated: 2022-05-02T06:26:26Z
has_accepted_license: '1'
intvolume: '         5'
isi: 1
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Communications Biology
publication_identifier:
  eissn:
  - 2399-3642
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Nutrient and salt depletion synergistically boosts glucose metabolism in individual
  Escherichia coli cells
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 5
year: '2022'
...
---
_id: '12009'
abstract:
- lang: eng
  text: Changes in the short-term dynamics of excitatory synapses over development
    have been observed throughout cortex, but their purpose and consequences remain
    unclear. Here, we propose that developmental changes in synaptic dynamics buffer
    the effect of slow inhibitory long-term plasticity, allowing for continuously
    stable neural activity. Using computational modeling we demonstrate that early
    in development excitatory short-term depression quickly stabilises neural activity,
    even in the face of strong, unbalanced excitation. We introduce a model of the
    commonly observed developmental shift from depression to facilitation and show
    that neural activity remains stable throughout development, while inhibitory synaptic
    plasticity slowly balances excitation, consistent with experimental observations.
    Our model predicts changes in the input responses from phasic to phasic-and-tonic
    and more precise spike timings. We also observe a gradual emergence of short-lasting
    memory traces governed by short-term plasticity development. We conclude that
    the developmental depression-to-facilitation shift may control excitation-inhibition
    balance throughout development with important functional consequences.
acknowledgement: We would like to thank the Vogels Lab for feedback on an earlier
  version of this manuscript. D.W.J. was supported by a Marshall Scholarship and a
  Clarendon Scholarship. R.P.C. and T.P.V. were supported by a Wellcome Trust and
  Royal Society Sir Henry Dale Fellowship (WT 100000), a Wellcome Trust Senior Research
  Fellowship (214316/Z/18/Z), and an ERC Consolidator Grant (SYNAPSEEK).
article_number: '873'
article_processing_charge: No
article_type: original
author:
- first_name: David W.
  full_name: Jia, David W.
  last_name: Jia
- first_name: Tim P
  full_name: Vogels, Tim P
  id: CB6FF8D2-008F-11EA-8E08-2637E6697425
  last_name: Vogels
  orcid: 0000-0003-3295-6181
- first_name: Rui Ponte
  full_name: Costa, Rui Ponte
  last_name: Costa
citation:
  ama: Jia DW, Vogels TP, Costa RP. Developmental depression-to-facilitation shift
    controls excitation-inhibition balance. <i>Communications biology</i>. 2022;5.
    doi:<a href="https://doi.org/10.1038/s42003-022-03801-2">10.1038/s42003-022-03801-2</a>
  apa: Jia, D. W., Vogels, T. P., &#38; Costa, R. P. (2022). Developmental depression-to-facilitation
    shift controls excitation-inhibition balance. <i>Communications Biology</i>. Springer
    Nature. <a href="https://doi.org/10.1038/s42003-022-03801-2">https://doi.org/10.1038/s42003-022-03801-2</a>
  chicago: Jia, David W., Tim P Vogels, and Rui Ponte Costa. “Developmental Depression-to-Facilitation
    Shift Controls Excitation-Inhibition Balance.” <i>Communications Biology</i>.
    Springer Nature, 2022. <a href="https://doi.org/10.1038/s42003-022-03801-2">https://doi.org/10.1038/s42003-022-03801-2</a>.
  ieee: D. W. Jia, T. P. Vogels, and R. P. Costa, “Developmental depression-to-facilitation
    shift controls excitation-inhibition balance,” <i>Communications biology</i>,
    vol. 5. Springer Nature, 2022.
  ista: Jia DW, Vogels TP, Costa RP. 2022. Developmental depression-to-facilitation
    shift controls excitation-inhibition balance. Communications biology. 5, 873.
  mla: Jia, David W., et al. “Developmental Depression-to-Facilitation Shift Controls
    Excitation-Inhibition Balance.” <i>Communications Biology</i>, vol. 5, 873, Springer
    Nature, 2022, doi:<a href="https://doi.org/10.1038/s42003-022-03801-2">10.1038/s42003-022-03801-2</a>.
  short: D.W. Jia, T.P. Vogels, R.P. Costa, Communications Biology 5 (2022).
date_created: 2022-09-04T22:02:02Z
date_published: 2022-08-25T00:00:00Z
date_updated: 2023-08-03T13:22:42Z
day: '25'
ddc:
- '570'
department:
- _id: TiVo
doi: 10.1038/s42003-022-03801-2
ec_funded: 1
external_id:
  isi:
  - '000844814800007'
file:
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  checksum: 3ec724c4f6d3440028c217305e32915f
  content_type: application/pdf
  creator: dernst
  date_created: 2022-09-05T08:55:11Z
  date_updated: 2022-09-05T08:55:11Z
  file_id: '12022'
  file_name: 2022_CommBiology_Jia.pdf
  file_size: 2491191
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  success: 1
file_date_updated: 2022-09-05T08:55:11Z
has_accepted_license: '1'
intvolume: '         5'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: c084a126-5a5b-11eb-8a69-d75314a70a87
  grant_number: 214316/Z/18/Z
  name: What’s in a memory? Spatiotemporal dynamics in strongly coupled recurrent
    neuronal networks.
- _id: 0aacfa84-070f-11eb-9043-d7eb2c709234
  call_identifier: H2020
  grant_number: '819603'
  name: Learning the shape of synaptic plasticity rules for neuronal architectures
    and function through machine learning.
publication: Communications biology
publication_identifier:
  eissn:
  - 2399-3642
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Developmental depression-to-facilitation shift controls excitation-inhibition
  balance
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 5
year: '2022'
...