[{"volume":6,"oa":1,"date_updated":"2023-12-13T12:07:33Z","article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We thank Marton Gulyas (ELTE Eötvös University) for development of videomicroscopy experiment manager and image analysis software. Authors are grateful to Gabor Forgacs (University of Missouri) for critical reading of earlier versions of this manuscript as well as to Zsuzsa Akos and Andras Czirok (ELTE Eötvös University) for fruitful discussions. This work was supported by EU FP7, ERC COLLMOT Project No 227878 to TV, the National Research Development and Innovation Fund of Hungary, K119359 and also Project No 2018-1.2.1-NKP-2018-00005 to LN. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 955576. MV was supported by the Ja´nos Bolyai Fellowship of the Hungarian Academy of Sciences.\r\nOpen access funding provided by Eötvös Loránd University.","oa_version":"Published Version","quality_controlled":"1","pmid":1,"_id":"14041","publication_identifier":{"eissn":["2399-3642"]},"publication_status":"published","citation":{"apa":"Méhes, E., Mones, E., Varga, M., Zsigmond, Á., Biri-Kovács, B., Nyitray, L., … Vicsek, T. (2023). 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-023-05181-7\">https://doi.org/10.1038/s42003-023-05181-7</a>","ieee":"E. Méhes <i>et al.</i>, “3D cell segregation geometry and dynamics are governed by tissue surface tension regulation,” <i>Communications Biology</i>, vol. 6. Springer Nature, 2023.","chicago":"Méhes, Elod, Enys Mones, Máté Varga, Áron Zsigmond, Beáta Biri-Kovács, László Nyitray, Vanessa Barone, Gabriel Krens, Carl-Philipp J Heisenberg, and Tamás Vicsek. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” <i>Communications Biology</i>. Springer Nature, 2023. <a href=\"https://doi.org/10.1038/s42003-023-05181-7\">https://doi.org/10.1038/s42003-023-05181-7</a>.","mla":"Méhes, Elod, et al. “3D Cell Segregation Geometry and Dynamics Are Governed by Tissue Surface Tension Regulation.” <i>Communications Biology</i>, vol. 6, 817, Springer Nature, 2023, doi:<a href=\"https://doi.org/10.1038/s42003-023-05181-7\">10.1038/s42003-023-05181-7</a>.","ama":"Méhes E, Mones E, Varga M, et al. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. <i>Communications Biology</i>. 2023;6. doi:<a href=\"https://doi.org/10.1038/s42003-023-05181-7\">10.1038/s42003-023-05181-7</a>","ista":"Méhes E, Mones E, Varga M, Zsigmond Á, Biri-Kovács B, Nyitray L, Barone V, Krens G, Heisenberg C-PJ, Vicsek T. 2023. 3D cell segregation geometry and dynamics are governed by tissue surface tension regulation. Communications Biology. 6, 817.","short":"E. Méhes, E. Mones, M. Varga, Á. Zsigmond, B. Biri-Kovács, L. Nyitray, V. Barone, G. Krens, C.-P.J. Heisenberg, T. Vicsek, Communications Biology 6 (2023)."},"author":[{"first_name":"Elod","last_name":"Méhes","full_name":"Méhes, Elod"},{"last_name":"Mones","full_name":"Mones, Enys","first_name":"Enys"},{"first_name":"Máté","last_name":"Varga","full_name":"Varga, Máté"},{"first_name":"Áron","last_name":"Zsigmond","full_name":"Zsigmond, Áron"},{"first_name":"Beáta","full_name":"Biri-Kovács, Beáta","last_name":"Biri-Kovács"},{"first_name":"László","full_name":"Nyitray, László","last_name":"Nyitray"},{"id":"419EECCC-F248-11E8-B48F-1D18A9856A87","last_name":"Barone","full_name":"Barone, Vanessa","orcid":"0000-0003-2676-3367","first_name":"Vanessa"},{"id":"2B819732-F248-11E8-B48F-1D18A9856A87","full_name":"Krens, Gabriel","last_name":"Krens","orcid":"0000-0003-4761-5996","first_name":"Gabriel"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","first_name":"Carl-Philipp J"},{"full_name":"Vicsek, Tamás","last_name":"Vicsek","first_name":"Tamás"}],"abstract":[{"text":"Tissue morphogenesis and patterning during development involve the segregation of cell types. Segregation is driven by differential tissue surface tensions generated by cell types through controlling cell-cell contact formation by regulating adhesion and actomyosin contractility-based cellular cortical tensions. We use vertebrate tissue cell types and zebrafish germ layer progenitors as in vitro models of 3-dimensional heterotypic segregation and developed a quantitative analysis of their dynamics based on 3D time-lapse microscopy. We show that general inhibition of actomyosin contractility by the Rho kinase inhibitor Y27632 delays segregation. Cell type-specific inhibition of non-muscle myosin2 activity by overexpression of myosin assembly inhibitor S100A4 reduces tissue surface tension, manifested in decreased compaction during aggregation and inverted geometry observed during segregation. The same is observed when we express a constitutively active Rho kinase isoform to ubiquitously keep actomyosin contractility high at cell-cell and cell-medium interfaces and thus overriding the interface-specific regulation of cortical tensions. Tissue surface tension regulation can become an effective tool in tissue engineering.","lang":"eng"}],"article_number":"817","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"doi":"10.1038/s42003-023-05181-7","year":"2023","external_id":{"isi":["001042544100001"],"pmid":["37542157"]},"title":"3D cell segregation geometry and dynamics are governed by tissue surface tension regulation","file_date_updated":"2023-08-14T07:17:36Z","publication":"Communications Biology","type":"journal_article","day":"04","status":"public","intvolume":"         6","department":[{"_id":"CaHe"},{"_id":"Bio"}],"has_accepted_license":"1","file":[{"date_created":"2023-08-14T07:17:36Z","checksum":"1f9324f736bdbb76426b07736651c4cd","file_name":"2023_CommBiology_Mehes.pdf","file_size":10181997,"date_updated":"2023-08-14T07:17:36Z","access_level":"open_access","success":1,"creator":"dernst","file_id":"14045","content_type":"application/pdf","relation":"main_file"}],"date_created":"2023-08-13T22:01:13Z","date_published":"2023-08-04T00:00:00Z","article_type":"original","month":"08","language":[{"iso":"eng"}],"publisher":"Springer Nature","scopus_import":"1"},{"day":"20","type":"journal_article","intvolume":"         5","status":"public","publication":"Communications Biology","file_date_updated":"2022-05-02T06:26:26Z","month":"04","date_published":"2022-04-20T00:00:00Z","article_type":"original","scopus_import":"1","publisher":"Springer Nature","language":[{"iso":"eng"}],"has_accepted_license":"1","department":[{"_id":"CaGu"}],"file":[{"checksum":"7c6f76ab17393d650825cc240edc84b3","date_created":"2022-05-02T06:26:26Z","file_size":2827723,"file_name":"2022_CommBiology_Glover.pdf","access_level":"open_access","date_updated":"2022-05-02T06:26:26Z","success":1,"creator":"dernst","file_id":"11342","relation":"main_file","content_type":"application/pdf"}],"date_created":"2022-05-01T22:01:41Z","citation":{"mla":"Glover, Georgina, et al. “Nutrient and Salt Depletion Synergistically Boosts Glucose Metabolism in Individual Escherichia Coli Cells.” <i>Communications Biology</i>, vol. 5, 385, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s42003-022-03336-6\">10.1038/s42003-022-03336-6</a>.","ama":"Glover G, Voliotis M, Łapińska U, et al. Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells. <i>Communications Biology</i>. 2022;5. doi:<a href=\"https://doi.org/10.1038/s42003-022-03336-6\">10.1038/s42003-022-03336-6</a>","short":"G. Glover, M. Voliotis, U. Łapińska, B.M. Invergo, D. Soanes, P. O’Neill, K. Moore, N. Nikolic, P. Petrov, D.S. Milner, S. Roy, K. Heesom, T.A. Richards, K. Tsaneva-Atanasova, S. Pagliara, Communications Biology 5 (2022).","ista":"Glover G, Voliotis M, Łapińska U, Invergo BM, Soanes D, O’Neill P, Moore K, Nikolic N, Petrov P, Milner DS, Roy S, Heesom K, Richards TA, Tsaneva-Atanasova K, Pagliara S. 2022. Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells. Communications Biology. 5, 385.","apa":"Glover, G., Voliotis, M., Łapińska, U., Invergo, B. M., Soanes, D., O’Neill, P., … Pagliara, S. (2022). Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-022-03336-6\">https://doi.org/10.1038/s42003-022-03336-6</a>","ieee":"G. Glover <i>et al.</i>, “Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells,” <i>Communications Biology</i>, vol. 5. Springer Nature, 2022.","chicago":"Glover, Georgina, Margaritis Voliotis, Urszula Łapińska, Brandon M. Invergo, Darren Soanes, Paul O’Neill, Karen Moore, et al. “Nutrient and Salt Depletion Synergistically Boosts Glucose Metabolism in Individual Escherichia Coli Cells.” <i>Communications Biology</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s42003-022-03336-6\">https://doi.org/10.1038/s42003-022-03336-6</a>."},"publication_status":"published","abstract":[{"lang":"eng","text":"The interaction between a cell and its environment shapes fundamental intracellular processes such as cellular metabolism. In most cases growth rate is treated as a proximal metric for understanding the cellular metabolic status. However, changes in growth rate might not reflect metabolic variations in individuals responding to environmental fluctuations. Here we use single-cell microfluidics-microscopy combined with transcriptomics, proteomics and mathematical modelling to quantify the accumulation of glucose within Escherichia coli cells. In contrast to the current consensus, we reveal that environmental conditions which are comparatively unfavourable for growth, where both nutrients and salinity are depleted, increase glucose accumulation rates in individual bacteria and population subsets. We find that these changes in metabolic function are underpinned by variations at the translational and posttranslational level but not at the transcriptional level and are not dictated by changes in cell size. The metabolic response-characteristics identified greatly advance our fundamental understanding of the interactions between bacteria and their environment and have important ramifications when investigating cellular processes where salinity plays an important role."}],"author":[{"first_name":"Georgina","last_name":"Glover","full_name":"Glover, Georgina"},{"first_name":"Margaritis","last_name":"Voliotis","full_name":"Voliotis, Margaritis"},{"last_name":"Łapińska","full_name":"Łapińska, Urszula","first_name":"Urszula"},{"full_name":"Invergo, Brandon M.","last_name":"Invergo","first_name":"Brandon M."},{"full_name":"Soanes, Darren","last_name":"Soanes","first_name":"Darren"},{"first_name":"Paul","last_name":"O’Neill","full_name":"O’Neill, Paul"},{"first_name":"Karen","last_name":"Moore","full_name":"Moore, Karen"},{"orcid":"0000-0001-9068-6090","last_name":"Nikolic","full_name":"Nikolic, Nela","first_name":"Nela","id":"42D9CABC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Peter","last_name":"Petrov","full_name":"Petrov, Peter"},{"first_name":"David S.","last_name":"Milner","full_name":"Milner, David S."},{"full_name":"Roy, Sumita","last_name":"Roy","first_name":"Sumita"},{"first_name":"Kate","full_name":"Heesom, Kate","last_name":"Heesom"},{"first_name":"Thomas A.","last_name":"Richards","full_name":"Richards, Thomas A."},{"full_name":"Tsaneva-Atanasova, Krasimira","last_name":"Tsaneva-Atanasova","first_name":"Krasimira"},{"last_name":"Pagliara","full_name":"Pagliara, Stefano","first_name":"Stefano"}],"article_processing_charge":"No","oa":1,"volume":5,"date_updated":"2023-08-03T06:45:26Z","publication_identifier":{"eissn":["2399-3642"]},"pmid":1,"_id":"11339","quality_controlled":"1","oa_version":"Published Version","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","acknowledgement":"G.G. was supported by an EPSRC DTP PhD studentship (EP/M506527/1). M.V. and K.T.A. gratefully acknowledge financial support from the EPSRC (EP/N014391/1). U.L. was supported through a BBSRC grant (BB/V008021/1) and an MRC Proximity to Discovery EXCITEME2 grant (MCPC17189). This work was further supported by a Royal Society Research Grant (RG180007) awarded to S.P. and a QUEX Initiator grant awarded to S.P. and K.T.A.. D.S.M., T.A.R. and S.P.’s work in this area is also supported by a Marie Skłodowska-Curie project SINGEK (H2020-MSCA-ITN-2015-675752) and the Gordon and Betty Moore Foundation Marine Microbiology Initiative (GBMF5514). B.M.I. acknowledges support from a Wellcome Trust Institutional Strategic Support Award to the University of Exeter (204909/Z/16/Z). This project utilised equipment funded by the Wellcome Trust Institutional Strategic Support Fund (WT097835MF), Wellcome Trust Multi User Equipment Award (WT101650MA) and BBSRC LOLA award (BB/K003240/1).","doi":"10.1038/s42003-022-03336-6","year":"2022","external_id":{"isi":["000784143400001"],"pmid":["35444215"]},"title":"Nutrient and salt depletion synergistically boosts glucose metabolism in individual Escherichia coli cells","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"isi":1,"article_number":"385","ddc":["570"]},{"ec_funded":1,"doi":"10.1038/s42003-022-03801-2","year":"2022","external_id":{"isi":["000844814800007"]},"title":"Developmental depression-to-facilitation shift controls excitation-inhibition balance","article_number":"873","isi":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"ddc":["570"],"publication_status":"published","citation":{"ieee":"D. W. Jia, T. P. Vogels, and R. P. Costa, “Developmental depression-to-facilitation shift controls excitation-inhibition balance,” <i>Communications biology</i>, vol. 5. Springer Nature, 2022.","apa":"Jia, D. W., Vogels, T. P., &#38; Costa, R. P. (2022). Developmental depression-to-facilitation shift controls excitation-inhibition balance. <i>Communications Biology</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s42003-022-03801-2\">https://doi.org/10.1038/s42003-022-03801-2</a>","chicago":"Jia, David W., Tim P Vogels, and Rui Ponte Costa. “Developmental Depression-to-Facilitation Shift Controls Excitation-Inhibition Balance.” <i>Communications Biology</i>. Springer Nature, 2022. <a href=\"https://doi.org/10.1038/s42003-022-03801-2\">https://doi.org/10.1038/s42003-022-03801-2</a>.","mla":"Jia, David W., et al. “Developmental Depression-to-Facilitation Shift Controls Excitation-Inhibition Balance.” <i>Communications Biology</i>, vol. 5, 873, Springer Nature, 2022, doi:<a href=\"https://doi.org/10.1038/s42003-022-03801-2\">10.1038/s42003-022-03801-2</a>.","ama":"Jia DW, Vogels TP, Costa RP. Developmental depression-to-facilitation shift controls excitation-inhibition balance. <i>Communications biology</i>. 2022;5. doi:<a href=\"https://doi.org/10.1038/s42003-022-03801-2\">10.1038/s42003-022-03801-2</a>","ista":"Jia DW, Vogels TP, Costa RP. 2022. Developmental depression-to-facilitation shift controls excitation-inhibition balance. Communications biology. 5, 873.","short":"D.W. Jia, T.P. Vogels, R.P. Costa, Communications Biology 5 (2022)."},"author":[{"full_name":"Jia, David W.","last_name":"Jia","first_name":"David W."},{"id":"CB6FF8D2-008F-11EA-8E08-2637E6697425","last_name":"Vogels","full_name":"Vogels, Tim P","orcid":"0000-0003-3295-6181","first_name":"Tim P"},{"first_name":"Rui Ponte","last_name":"Costa","full_name":"Costa, Rui Ponte"}],"abstract":[{"lang":"eng","text":"Changes in the short-term dynamics of excitatory synapses over development have been observed throughout cortex, but their purpose and consequences remain unclear. Here, we propose that developmental changes in synaptic dynamics buffer the effect of slow inhibitory long-term plasticity, allowing for continuously stable neural activity. Using computational modeling we demonstrate that early in development excitatory short-term depression quickly stabilises neural activity, even in the face of strong, unbalanced excitation. We introduce a model of the commonly observed developmental shift from depression to facilitation and show that neural activity remains stable throughout development, while inhibitory synaptic plasticity slowly balances excitation, consistent with experimental observations. Our model predicts changes in the input responses from phasic to phasic-and-tonic and more precise spike timings. We also observe a gradual emergence of short-lasting memory traces governed by short-term plasticity development. We conclude that the developmental depression-to-facilitation shift may control excitation-inhibition balance throughout development with important functional consequences."}],"date_updated":"2023-08-03T13:22:42Z","oa":1,"volume":5,"article_processing_charge":"No","acknowledgement":"We would like to thank the Vogels Lab for feedback on an earlier version of this manuscript. D.W.J. was supported by a Marshall Scholarship and a Clarendon Scholarship. R.P.C. and T.P.V. were supported by a Wellcome Trust and Royal Society Sir Henry Dale Fellowship (WT 100000), a Wellcome Trust Senior Research Fellowship (214316/Z/18/Z), and an ERC Consolidator Grant (SYNAPSEEK).","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","project":[{"grant_number":"214316/Z/18/Z","name":"What’s in a memory? Spatiotemporal dynamics in strongly coupled recurrent neuronal networks.","_id":"c084a126-5a5b-11eb-8a69-d75314a70a87"},{"call_identifier":"H2020","name":"Learning the shape of synaptic plasticity rules for neuronal architectures and function through machine learning.","_id":"0aacfa84-070f-11eb-9043-d7eb2c709234","grant_number":"819603"}],"oa_version":"Published Version","_id":"12009","publication_identifier":{"eissn":["2399-3642"]},"article_type":"original","date_published":"2022-08-25T00:00:00Z","month":"08","language":[{"iso":"eng"}],"publisher":"Springer Nature","scopus_import":"1","department":[{"_id":"TiVo"}],"has_accepted_license":"1","date_created":"2022-09-04T22:02:02Z","file":[{"content_type":"application/pdf","relation":"main_file","file_id":"12022","creator":"dernst","success":1,"date_updated":"2022-09-05T08:55:11Z","access_level":"open_access","file_name":"2022_CommBiology_Jia.pdf","file_size":2491191,"date_created":"2022-09-05T08:55:11Z","checksum":"3ec724c4f6d3440028c217305e32915f"}],"type":"journal_article","day":"25","status":"public","intvolume":"         5","file_date_updated":"2022-09-05T08:55:11Z","publication":"Communications biology"}]