---
_id: '9361'
abstract:
- lang: eng
  text: The multimeric matrix (M) protein of clinically relevant paramyxoviruses orchestrates
    assembly and budding activity of viral particles at the plasma membrane (PM).
    We identified within the canine distemper virus (CDV) M protein two microdomains,
    potentially assuming α-helix structures, which are essential for membrane budding
    activity. Remarkably, while two rationally designed microdomain M mutants (E89R,
    microdomain 1 and L239D, microdomain 2) preserved proper folding, dimerization,
    interaction with the nucleocapsid protein, localization at and deformation of
    the PM, the virus-like particle formation, as well as production of infectious
    virions (as monitored using a membrane budding-complementation system), were,
    in sharp contrast, strongly impaired. Of major importance, raster image correlation
    spectroscopy (RICS) revealed that both microdomains contributed to finely tune
    M protein mobility specifically at the PM. Collectively, our data highlighted
    the cornerstone membrane budding-priming activity of two spatially discrete M
    microdomains, potentially by coordinating the assembly of productive higher oligomers
    at the PM.
acknowledgement: This work was supported by the Swiss National Science Foundation
  (referencenumber 310030_173185 to P. P.).
article_number: e01024-20
article_processing_charge: No
author:
- first_name: Matthieu
  full_name: Gast, Matthieu
  last_name: Gast
- first_name: Nicole P.
  full_name: Kadzioch, Nicole P.
  last_name: Kadzioch
- first_name: Doreen
  full_name: Milius, Doreen
  id: 384050BC-F248-11E8-B48F-1D18A9856A87
  last_name: Milius
- first_name: Francesco
  full_name: Origgi, Francesco
  last_name: Origgi
- first_name: Philippe
  full_name: Plattet, Philippe
  last_name: Plattet
citation:
  ama: Gast M, Kadzioch NP, Milius D, Origgi F, Plattet P. Oligomerization and cell
    egress controlled by two microdomains of canine distemper virus matrix protein.
    <i>mSphere</i>. 2021;6(2). doi:<a href="https://doi.org/10.1128/mSphere.01024-20">10.1128/mSphere.01024-20</a>
  apa: Gast, M., Kadzioch, N. P., Milius, D., Origgi, F., &#38; Plattet, P. (2021).
    Oligomerization and cell egress controlled by two microdomains of canine distemper
    virus matrix protein. <i>MSphere</i>. American Society for Microbiology. <a href="https://doi.org/10.1128/mSphere.01024-20">https://doi.org/10.1128/mSphere.01024-20</a>
  chicago: Gast, Matthieu, Nicole P. Kadzioch, Doreen Milius, Francesco Origgi, and
    Philippe Plattet. “Oligomerization and Cell Egress Controlled by Two Microdomains
    of Canine Distemper Virus Matrix Protein.” <i>MSphere</i>. American Society for
    Microbiology, 2021. <a href="https://doi.org/10.1128/mSphere.01024-20">https://doi.org/10.1128/mSphere.01024-20</a>.
  ieee: M. Gast, N. P. Kadzioch, D. Milius, F. Origgi, and P. Plattet, “Oligomerization
    and cell egress controlled by two microdomains of canine distemper virus matrix
    protein,” <i>mSphere</i>, vol. 6, no. 2. American Society for Microbiology, 2021.
  ista: Gast M, Kadzioch NP, Milius D, Origgi F, Plattet P. 2021. Oligomerization
    and cell egress controlled by two microdomains of canine distemper virus matrix
    protein. mSphere. 6(2), e01024-20.
  mla: Gast, Matthieu, et al. “Oligomerization and Cell Egress Controlled by Two Microdomains
    of Canine Distemper Virus Matrix Protein.” <i>MSphere</i>, vol. 6, no. 2, e01024-20,
    American Society for Microbiology, 2021, doi:<a href="https://doi.org/10.1128/mSphere.01024-20">10.1128/mSphere.01024-20</a>.
  short: M. Gast, N.P. Kadzioch, D. Milius, F. Origgi, P. Plattet, MSphere 6 (2021).
date_created: 2021-05-02T22:01:28Z
date_published: 2021-04-14T00:00:00Z
date_updated: 2023-08-08T13:26:12Z
day: '14'
ddc:
- '570'
department:
- _id: Bio
doi: 10.1128/mSphere.01024-20
external_id:
  isi:
  - '000663823400025'
  pmid:
  - '33853875'
file:
- access_level: open_access
  checksum: 310748d140c8838335c1314431095898
  content_type: application/pdf
  creator: kschuh
  date_created: 2021-05-04T12:41:38Z
  date_updated: 2021-05-04T12:41:38Z
  file_id: '9370'
  file_name: 2021_mSphere_Gast.pdf
  file_size: 3379349
  relation: main_file
  success: 1
file_date_updated: 2021-05-04T12:41:38Z
has_accepted_license: '1'
intvolume: '         6'
isi: 1
issue: '2'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: mSphere
publication_identifier:
  eissn:
  - '23795042'
publication_status: published
publisher: American Society for Microbiology
quality_controlled: '1'
scopus_import: '1'
status: public
title: Oligomerization and cell egress controlled by two microdomains of canine distemper
  virus matrix protein
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2021'
...