[{"year":"2021","citation":{"ista":"Gast M, Kadzioch NP, Milius D, Origgi F, Plattet P. 2021. Oligomerization and cell egress controlled by two microdomains of canine distemper virus matrix protein. mSphere. 6(2), e01024-20.","mla":"Gast, Matthieu, et al. “Oligomerization and Cell Egress Controlled by Two Microdomains of Canine Distemper Virus Matrix Protein.” <i>MSphere</i>, vol. 6, no. 2, e01024-20, American Society for Microbiology, 2021, doi:<a href=\"https://doi.org/10.1128/mSphere.01024-20\">10.1128/mSphere.01024-20</a>.","short":"M. Gast, N.P. Kadzioch, D. Milius, F. Origgi, P. Plattet, MSphere 6 (2021).","chicago":"Gast, Matthieu, Nicole P. Kadzioch, Doreen Milius, Francesco Origgi, and Philippe Plattet. “Oligomerization and Cell Egress Controlled by Two Microdomains of Canine Distemper Virus Matrix Protein.” <i>MSphere</i>. American Society for Microbiology, 2021. <a href=\"https://doi.org/10.1128/mSphere.01024-20\">https://doi.org/10.1128/mSphere.01024-20</a>.","ieee":"M. Gast, N. P. Kadzioch, D. Milius, F. Origgi, and P. Plattet, “Oligomerization and cell egress controlled by two microdomains of canine distemper virus matrix protein,” <i>mSphere</i>, vol. 6, no. 2. American Society for Microbiology, 2021.","ama":"Gast M, Kadzioch NP, Milius D, Origgi F, Plattet P. Oligomerization and cell egress controlled by two microdomains of canine distemper virus matrix protein. <i>mSphere</i>. 2021;6(2). doi:<a href=\"https://doi.org/10.1128/mSphere.01024-20\">10.1128/mSphere.01024-20</a>","apa":"Gast, M., Kadzioch, N. P., Milius, D., Origgi, F., &#38; Plattet, P. (2021). Oligomerization and cell egress controlled by two microdomains of canine distemper virus matrix protein. <i>MSphere</i>. American Society for Microbiology. <a href=\"https://doi.org/10.1128/mSphere.01024-20\">https://doi.org/10.1128/mSphere.01024-20</a>"},"date_updated":"2023-08-08T13:26:12Z","external_id":{"pmid":["33853875"],"isi":["000663823400025"]},"isi":1,"day":"14","doi":"10.1128/mSphere.01024-20","abstract":[{"lang":"eng","text":"The multimeric matrix (M) protein of clinically relevant paramyxoviruses orchestrates assembly and budding activity of viral particles at the plasma membrane (PM). We identified within the canine distemper virus (CDV) M protein two microdomains, potentially assuming α-helix structures, which are essential for membrane budding activity. Remarkably, while two rationally designed microdomain M mutants (E89R, microdomain 1 and L239D, microdomain 2) preserved proper folding, dimerization, interaction with the nucleocapsid protein, localization at and deformation of the PM, the virus-like particle formation, as well as production of infectious virions (as monitored using a membrane budding-complementation system), were, in sharp contrast, strongly impaired. Of major importance, raster image correlation spectroscopy (RICS) revealed that both microdomains contributed to finely tune M protein mobility specifically at the PM. Collectively, our data highlighted the cornerstone membrane budding-priming activity of two spatially discrete M microdomains, potentially by coordinating the assembly of productive higher oligomers at the PM."}],"acknowledgement":"This work was supported by the Swiss National Science Foundation (referencenumber 310030_173185 to P. P.).","volume":6,"ddc":["570"],"scopus_import":"1","_id":"9361","pmid":1,"issue":"2","author":[{"first_name":"Matthieu","last_name":"Gast","full_name":"Gast, Matthieu"},{"full_name":"Kadzioch, Nicole P.","first_name":"Nicole P.","last_name":"Kadzioch"},{"last_name":"Milius","first_name":"Doreen","full_name":"Milius, Doreen","id":"384050BC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Francesco","last_name":"Origgi","full_name":"Origgi, Francesco"},{"last_name":"Plattet","first_name":"Philippe","full_name":"Plattet, Philippe"}],"date_created":"2021-05-02T22:01:28Z","department":[{"_id":"Bio"}],"article_processing_charge":"No","publication_status":"published","intvolume":"         6","title":"Oligomerization and cell egress controlled by two microdomains of canine distemper virus matrix protein","quality_controlled":"1","file_date_updated":"2021-05-04T12:41:38Z","publisher":"American Society for Microbiology","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","date_published":"2021-04-14T00:00:00Z","publication_identifier":{"eissn":["23795042"]},"oa":1,"file":[{"relation":"main_file","success":1,"access_level":"open_access","creator":"kschuh","file_id":"9370","checksum":"310748d140c8838335c1314431095898","file_size":3379349,"date_created":"2021-05-04T12:41:38Z","file_name":"2021_mSphere_Gast.pdf","content_type":"application/pdf","date_updated":"2021-05-04T12:41:38Z"}],"status":"public","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","has_accepted_license":"1","publication":"mSphere","oa_version":"Published Version","article_number":"e01024-20","month":"04","language":[{"iso":"eng"}]}]