---
_id: '6029'
abstract:
- lang: eng
  text: Protein micropatterning has become an important tool for many biomedical applications
    as well as in academic research. Current techniques that allow to reduce the feature
    size of patterns below 1 μm are, however, often costly and require sophisticated
    equipment. We present here a straightforward and convenient method to generate
    highly condensed nanopatterns of proteins without the need for clean room facilities
    or expensive equipment. Our approach is based on nanocontact printing and allows
    for the fabrication of protein patterns with feature sizes of 80 nm and periodicities
    down to 140 nm. This was made possible by the use of the material X-poly(dimethylsiloxane)
    (X-PDMS) in a two-layer stamp layout for protein printing. In a proof of principle,
    different proteins at various scales were printed and the pattern quality was
    evaluated by atomic force microscopy (AFM) and super-resolution fluorescence microscopy.
article_number: '655'
article_processing_charge: No
author:
- first_name: Marco
  full_name: Lindner, Marco
  last_name: Lindner
- first_name: Aliz
  full_name: Tresztenyak, Aliz
  last_name: Tresztenyak
- first_name: Gergö
  full_name: Fülöp, Gergö
  last_name: Fülöp
- first_name: Wiebke
  full_name: Jahr, Wiebke
  id: 425C1CE8-F248-11E8-B48F-1D18A9856A87
  last_name: Jahr
- first_name: Adrian
  full_name: Prinz, Adrian
  last_name: Prinz
- first_name: Iris
  full_name: Prinz, Iris
  last_name: Prinz
- first_name: Johann G
  full_name: Danzl, Johann G
  id: 42EFD3B6-F248-11E8-B48F-1D18A9856A87
  last_name: Danzl
  orcid: 0000-0001-8559-3973
- first_name: Gerhard J.
  full_name: Schütz, Gerhard J.
  last_name: Schütz
- first_name: Eva
  full_name: Sevcsik, Eva
  last_name: Sevcsik
citation:
  ama: Lindner M, Tresztenyak A, Fülöp G, et al. A fast and simple contact printing
    approach to generate 2D protein nanopatterns. <i>Frontiers in Chemistry</i>. 2019;6.
    doi:<a href="https://doi.org/10.3389/fchem.2018.00655">10.3389/fchem.2018.00655</a>
  apa: Lindner, M., Tresztenyak, A., Fülöp, G., Jahr, W., Prinz, A., Prinz, I., …
    Sevcsik, E. (2019). A fast and simple contact printing approach to generate 2D
    protein nanopatterns. <i>Frontiers in Chemistry</i>. Frontiers Media S.A. <a href="https://doi.org/10.3389/fchem.2018.00655">https://doi.org/10.3389/fchem.2018.00655</a>
  chicago: Lindner, Marco, Aliz Tresztenyak, Gergö Fülöp, Wiebke Jahr, Adrian Prinz,
    Iris Prinz, Johann G Danzl, Gerhard J. Schütz, and Eva Sevcsik. “A Fast and Simple
    Contact Printing Approach to Generate 2D Protein Nanopatterns.” <i>Frontiers in
    Chemistry</i>. Frontiers Media S.A., 2019. <a href="https://doi.org/10.3389/fchem.2018.00655">https://doi.org/10.3389/fchem.2018.00655</a>.
  ieee: M. Lindner <i>et al.</i>, “A fast and simple contact printing approach to
    generate 2D protein nanopatterns,” <i>Frontiers in Chemistry</i>, vol. 6. Frontiers
    Media S.A., 2019.
  ista: Lindner M, Tresztenyak A, Fülöp G, Jahr W, Prinz A, Prinz I, Danzl JG, Schütz
    GJ, Sevcsik E. 2019. A fast and simple contact printing approach to generate 2D
    protein nanopatterns. Frontiers in Chemistry. 6, 655.
  mla: Lindner, Marco, et al. “A Fast and Simple Contact Printing Approach to Generate
    2D Protein Nanopatterns.” <i>Frontiers in Chemistry</i>, vol. 6, 655, Frontiers
    Media S.A., 2019, doi:<a href="https://doi.org/10.3389/fchem.2018.00655">10.3389/fchem.2018.00655</a>.
  short: M. Lindner, A. Tresztenyak, G. Fülöp, W. Jahr, A. Prinz, I. Prinz, J.G. Danzl,
    G.J. Schütz, E. Sevcsik, Frontiers in Chemistry 6 (2019).
date_created: 2019-02-17T22:59:24Z
date_published: 2019-01-24T00:00:00Z
date_updated: 2023-08-24T14:45:38Z
day: '24'
ddc:
- '540'
department:
- _id: JoDa
doi: 10.3389/fchem.2018.00655
external_id:
  isi:
  - '000456718000001'
file:
- access_level: open_access
  checksum: 7841301d7c53b56ef873791b4b6f7b24
  content_type: application/pdf
  creator: dernst
  date_created: 2019-02-18T15:10:34Z
  date_updated: 2020-07-14T12:47:17Z
  file_id: '6039'
  file_name: 2019_frontiers_Lindner.pdf
  file_size: 1766820
  relation: main_file
file_date_updated: 2020-07-14T12:47:17Z
has_accepted_license: '1'
intvolume: '         6'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Frontiers in Chemistry
publication_identifier:
  eissn:
  - '22962646'
publication_status: published
publisher: Frontiers Media S.A.
quality_controlled: '1'
scopus_import: '1'
status: public
title: A fast and simple contact printing approach to generate 2D protein nanopatterns
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 6
year: '2019'
...