@article{7600,
  abstract     = {Directional intercellular transport of the phytohormone auxin mediated by PIN FORMED (PIN) efflux carriers plays essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes. Multilevel regulations of PIN activity under internal and external cues are complicated; however, the underlying molecular mechanism remains elusive. Here we demonstrate that 3’-Phosphoinositide-Dependent Protein Kinase1 (PDK1), which is conserved in plants and mammals, functions as a molecular hub integrating the upstream lipid signalling and the downstream substrate activity through phosphorylation. Genetic analysis uncovers that loss-of-function Arabidopsis mutant pdk1.1 pdk1.2 exhibits a plethora of abnormalities in organogenesis and growth, due to the defective PIN-dependent auxin transport. Further cellular and biochemical analyses reveal that PDK1 phosphorylates D6 Protein Kinase to facilitate its activity towards PIN proteins. Our studies establish a lipid-dependent phosphorylation cascade connecting membrane composition-based cellular signalling with plant growth and patterning by regulating morphogenetic auxin fluxes.},
  author       = {Tan, Shutang and Zhang, Xixi and Kong, Wei and Yang, Xiao-Li and Molnar, Gergely and Vondráková, Zuzana and Filepová, Roberta and Petrášek, Jan and Friml, Jiří and Xue, Hong-Wei},
  issn         = {20550278},
  journal      = {Nature Plants},
  pages        = {556--569},
  publisher    = {Springer Nature},
  title        = {{The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis}},
  doi          = {10.1038/s41477-020-0648-9},
  volume       = {6},
  year         = {2020},
}