---
_id: '6412'
abstract:
- lang: eng
  text: Polycomb group (PcG) proteins play critical roles in the epigenetic inheritance
    of cell fate. The Polycomb Repressive Complexes PRC1 and PRC2 catalyse distinct
    chromatin modifications to enforce gene silencing, but how transcriptional repression
    is propagated through mitotic cell divisions remains a key unresolved question.
    Using reversible tethering of PcG proteins to ectopic sites in mouse embryonic
    stem cells, here we show that PRC1 can trigger transcriptional repression and
    Polycomb-dependent chromatin modifications. We find that canonical PRC1 (cPRC1),
    but not variant PRC1, maintains gene silencing through cell division upon reversal
    of tethering. Propagation of gene repression is sustained by cis-acting histone
    modifications, PRC2-mediated H3K27me3 and cPRC1-mediated H2AK119ub1, promoting
    a sequence-independent feedback mechanism for PcG protein recruitment. Thus, the
    distinct PRC1 complexes present in vertebrates can differentially regulate epigenetic
    maintenance of gene silencing, potentially enabling dynamic heritable responses
    to complex stimuli. Our findings reveal how PcG repression is potentially inherited
    in vertebrates.
article_number: '1931'
article_processing_charge: No
author:
- first_name: Hagar F.
  full_name: Moussa, Hagar F.
  last_name: Moussa
- first_name: Daniel
  full_name: Bsteh, Daniel
  last_name: Bsteh
- first_name: Ramesh
  full_name: Yelagandula, Ramesh
  last_name: Yelagandula
- first_name: Carina
  full_name: Pribitzer, Carina
  last_name: Pribitzer
- first_name: Karin
  full_name: Stecher, Karin
  last_name: Stecher
- first_name: Katarina
  full_name: Bartalska, Katarina
  id: 4D883232-F248-11E8-B48F-1D18A9856A87
  last_name: Bartalska
- first_name: Luca
  full_name: Michetti, Luca
  last_name: Michetti
- first_name: Jingkui
  full_name: Wang, Jingkui
  last_name: Wang
- first_name: Jorge A.
  full_name: Zepeda-Martinez, Jorge A.
  last_name: Zepeda-Martinez
- first_name: Ulrich
  full_name: Elling, Ulrich
  last_name: Elling
- first_name: Jacob I.
  full_name: Stuckey, Jacob I.
  last_name: Stuckey
- first_name: Lindsey I.
  full_name: James, Lindsey I.
  last_name: James
- first_name: Stephen V.
  full_name: Frye, Stephen V.
  last_name: Frye
- first_name: Oliver
  full_name: Bell, Oliver
  last_name: Bell
citation:
  ama: Moussa HF, Bsteh D, Yelagandula R, et al. Canonical PRC1 controls sequence-independent
    propagation of Polycomb-mediated gene silencing. <i>Nature Communications</i>.
    2019;10(1). doi:<a href="https://doi.org/10.1038/s41467-019-09628-6">10.1038/s41467-019-09628-6</a>
  apa: Moussa, H. F., Bsteh, D., Yelagandula, R., Pribitzer, C., Stecher, K., Bartalska,
    K., … Bell, O. (2019). Canonical PRC1 controls sequence-independent propagation
    of Polycomb-mediated gene silencing. <i>Nature Communications</i>. Springer Nature.
    <a href="https://doi.org/10.1038/s41467-019-09628-6">https://doi.org/10.1038/s41467-019-09628-6</a>
  chicago: Moussa, Hagar F., Daniel Bsteh, Ramesh Yelagandula, Carina Pribitzer, Karin
    Stecher, Katarina Bartalska, Luca Michetti, et al. “Canonical PRC1 Controls Sequence-Independent
    Propagation of Polycomb-Mediated Gene Silencing.” <i>Nature Communications</i>.
    Springer Nature, 2019. <a href="https://doi.org/10.1038/s41467-019-09628-6">https://doi.org/10.1038/s41467-019-09628-6</a>.
  ieee: H. F. Moussa <i>et al.</i>, “Canonical PRC1 controls sequence-independent
    propagation of Polycomb-mediated gene silencing,” <i>Nature Communications</i>,
    vol. 10, no. 1. Springer Nature, 2019.
  ista: Moussa HF, Bsteh D, Yelagandula R, Pribitzer C, Stecher K, Bartalska K, Michetti
    L, Wang J, Zepeda-Martinez JA, Elling U, Stuckey JI, James LI, Frye SV, Bell O.
    2019. Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated
    gene silencing. Nature Communications. 10(1), 1931.
  mla: Moussa, Hagar F., et al. “Canonical PRC1 Controls Sequence-Independent Propagation
    of Polycomb-Mediated Gene Silencing.” <i>Nature Communications</i>, vol. 10, no.
    1, 1931, Springer Nature, 2019, doi:<a href="https://doi.org/10.1038/s41467-019-09628-6">10.1038/s41467-019-09628-6</a>.
  short: H.F. Moussa, D. Bsteh, R. Yelagandula, C. Pribitzer, K. Stecher, K. Bartalska,
    L. Michetti, J. Wang, J.A. Zepeda-Martinez, U. Elling, J.I. Stuckey, L.I. James,
    S.V. Frye, O. Bell, Nature Communications 10 (2019).
date_created: 2019-05-13T07:58:35Z
date_published: 2019-04-29T00:00:00Z
date_updated: 2023-08-25T10:31:56Z
day: '29'
ddc:
- '570'
department:
- _id: SaSi
doi: 10.1038/s41467-019-09628-6
external_id:
  isi:
  - '000466118700002'
file:
- access_level: open_access
  checksum: 6550a328335396c856db4cbdda7d2994
  content_type: application/pdf
  creator: dernst
  date_created: 2019-05-14T08:45:51Z
  date_updated: 2020-07-14T12:47:29Z
  file_id: '6448'
  file_name: 2019_NatureComm_Moussa.pdf
  file_size: 1223647
  relation: main_file
file_date_updated: 2020-07-14T12:47:29Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
issue: '1'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
  eissn:
  - '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Canonical PRC1 controls sequence-independent propagation of Polycomb-mediated
  gene silencing
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2019'
...
---
_id: '6465'
abstract:
- lang: eng
  text: Tight control over protein degradation is a fundamental requirement for cells
    to respond rapidly to various stimuli and adapt to a fluctuating environment.
    Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons)
    for the precise regulation of protein expression profiles in mammalian cells by
    modulating target protein half-lives in a predictable manner. Using the well-established
    tetracycline gene-regulation system as a model, we show that the dynamics of protein
    expression can be tuned by fusing appropriate degron tags to gene regulators.
    Next, we apply this degron library to tune a synthetic pulse-generating circuit
    in mammalian cells. With this toolbox we establish a set of pulse generators with
    tailored pulse lengths and magnitudes of protein expression. This methodology
    will prove useful in the functional roles of essential proteins, fine-tuning of
    gene-expression systems, and enabling a higher complexity in the design of synthetic
    biological systems in mammalian cells.
article_number: '2013'
article_processing_charge: No
author:
- first_name: Hélène
  full_name: Chassin, Hélène
  last_name: Chassin
- first_name: Marius
  full_name: Müller, Marius
  last_name: Müller
- first_name: Marcel
  full_name: Tigges, Marcel
  last_name: Tigges
- first_name: Leo
  full_name: Scheller, Leo
  last_name: Scheller
- first_name: Moritz
  full_name: Lang, Moritz
  id: 29E0800A-F248-11E8-B48F-1D18A9856A87
  last_name: Lang
- first_name: Martin
  full_name: Fussenegger, Martin
  last_name: Fussenegger
citation:
  ama: Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. A modular
    degron library for synthetic circuits in mammalian cells. <i>Nature Communications</i>.
    2019;10(1). doi:<a href="https://doi.org/10.1038/s41467-019-09974-5">10.1038/s41467-019-09974-5</a>
  apa: Chassin, H., Müller, M., Tigges, M., Scheller, L., Lang, M., &#38; Fussenegger,
    M. (2019). A modular degron library for synthetic circuits in mammalian cells.
    <i>Nature Communications</i>. Springer Nature. <a href="https://doi.org/10.1038/s41467-019-09974-5">https://doi.org/10.1038/s41467-019-09974-5</a>
  chicago: Chassin, Hélène, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang,
    and Martin Fussenegger. “A Modular Degron Library for Synthetic Circuits in Mammalian
    Cells.” <i>Nature Communications</i>. Springer Nature, 2019. <a href="https://doi.org/10.1038/s41467-019-09974-5">https://doi.org/10.1038/s41467-019-09974-5</a>.
  ieee: H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, and M. Fussenegger,
    “A modular degron library for synthetic circuits in mammalian cells,” <i>Nature
    Communications</i>, vol. 10, no. 1. Springer Nature, 2019.
  ista: Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. 2019. A
    modular degron library for synthetic circuits in mammalian cells. Nature Communications.
    10(1), 2013.
  mla: Chassin, Hélène, et al. “A Modular Degron Library for Synthetic Circuits in
    Mammalian Cells.” <i>Nature Communications</i>, vol. 10, no. 1, 2013, Springer
    Nature, 2019, doi:<a href="https://doi.org/10.1038/s41467-019-09974-5">10.1038/s41467-019-09974-5</a>.
  short: H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger, Nature
    Communications 10 (2019).
date_created: 2019-05-19T21:59:14Z
date_published: 2019-05-01T00:00:00Z
date_updated: 2023-08-25T10:33:51Z
day: '01'
ddc:
- '570'
department:
- _id: CaGu
doi: 10.1038/s41467-019-09974-5
external_id:
  isi:
  - '000466338600006'
file:
- access_level: open_access
  checksum: e214d3e4f8c81e35981583c4569b51b8
  content_type: application/pdf
  creator: dernst
  date_created: 2019-05-20T07:33:54Z
  date_updated: 2020-07-14T12:47:31Z
  file_id: '6471'
  file_name: 2019_NatureComm_Chassin.pdf
  file_size: 1191827
  relation: main_file
file_date_updated: 2020-07-14T12:47:31Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
issue: '1'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
publication: Nature Communications
publication_identifier:
  eissn:
  - '20411723'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
  link:
  - relation: erratum
    url: https://doi.org/10.1038/s41467-023-36111-0
scopus_import: '1'
status: public
title: A modular degron library for synthetic circuits in mammalian cells
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2019'
...
---
_id: '6899'
abstract:
- lang: eng
  text: Intra-organ communication guides morphogenetic processes that are essential
    for an organ to carry out complex physiological functions. In the heart, the growth
    of the myocardium is tightly coupled to that of the endocardium, a specialized
    endothelial tissue that lines its interior. Several molecular pathways have been
    implicated in the communication between these tissues including secreted factors,
    components of the extracellular matrix, or proteins involved in cell-cell communication.
    Yet, it is unknown how the growth of the endocardium is coordinated with that
    of the myocardium. Here, we show that an increased expansion of the myocardial
    atrial chamber volume generates higher junctional forces within endocardial cells.
    This leads to biomechanical signaling involving VE-cadherin, triggering nuclear
    localization of the Hippo pathway transcriptional regulator Yap1 and endocardial
    proliferation. Our work suggests that the growth of the endocardium results from
    myocardial chamber volume expansion and ends when the tension on the tissue is
    relaxed.
article_processing_charge: No
author:
- first_name: Dorothee
  full_name: Bornhorst, Dorothee
  last_name: Bornhorst
- first_name: Peng
  full_name: Xia, Peng
  id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
  last_name: Xia
  orcid: 0000-0002-5419-7756
- first_name: Hiroyuki
  full_name: Nakajima, Hiroyuki
  last_name: Nakajima
- first_name: Chaitanya
  full_name: Dingare, Chaitanya
  last_name: Dingare
- first_name: Wiebke
  full_name: Herzog, Wiebke
  last_name: Herzog
- first_name: Virginie
  full_name: Lecaudey, Virginie
  last_name: Lecaudey
- first_name: Naoki
  full_name: Mochizuki, Naoki
  last_name: Mochizuki
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Deborah
  full_name: Yelon, Deborah
  last_name: Yelon
- first_name: Salim
  full_name: Abdelilah-Seyfried, Salim
  last_name: Abdelilah-Seyfried
citation:
  ama: Bornhorst D, Xia P, Nakajima H, et al. Biomechanical signaling within the developing
    zebrafish heart attunes endocardial growth to myocardial chamber dimensions. <i>Nature
    communications</i>. 2019;10(1):4113. doi:<a href="https://doi.org/10.1038/s41467-019-12068-x">10.1038/s41467-019-12068-x</a>
  apa: Bornhorst, D., Xia, P., Nakajima, H., Dingare, C., Herzog, W., Lecaudey, V.,
    … Abdelilah-Seyfried, S. (2019). Biomechanical signaling within the developing
    zebrafish heart attunes endocardial growth to myocardial chamber dimensions. <i>Nature
    Communications</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/s41467-019-12068-x">https://doi.org/10.1038/s41467-019-12068-x</a>
  chicago: Bornhorst, Dorothee, Peng Xia, Hiroyuki Nakajima, Chaitanya Dingare, Wiebke
    Herzog, Virginie Lecaudey, Naoki Mochizuki, Carl-Philipp J Heisenberg, Deborah
    Yelon, and Salim Abdelilah-Seyfried. “Biomechanical Signaling within the Developing
    Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.”
    <i>Nature Communications</i>. Nature Publishing Group, 2019. <a href="https://doi.org/10.1038/s41467-019-12068-x">https://doi.org/10.1038/s41467-019-12068-x</a>.
  ieee: D. Bornhorst <i>et al.</i>, “Biomechanical signaling within the developing
    zebrafish heart attunes endocardial growth to myocardial chamber dimensions,”
    <i>Nature communications</i>, vol. 10, no. 1. Nature Publishing Group, p. 4113,
    2019.
  ista: Bornhorst D, Xia P, Nakajima H, Dingare C, Herzog W, Lecaudey V, Mochizuki
    N, Heisenberg C-PJ, Yelon D, Abdelilah-Seyfried S. 2019. Biomechanical signaling
    within the developing zebrafish heart attunes endocardial growth to myocardial
    chamber dimensions. Nature communications. 10(1), 4113.
  mla: Bornhorst, Dorothee, et al. “Biomechanical Signaling within the Developing
    Zebrafish Heart Attunes Endocardial Growth to Myocardial Chamber Dimensions.”
    <i>Nature Communications</i>, vol. 10, no. 1, Nature Publishing Group, 2019, p.
    4113, doi:<a href="https://doi.org/10.1038/s41467-019-12068-x">10.1038/s41467-019-12068-x</a>.
  short: D. Bornhorst, P. Xia, H. Nakajima, C. Dingare, W. Herzog, V. Lecaudey, N.
    Mochizuki, C.-P.J. Heisenberg, D. Yelon, S. Abdelilah-Seyfried, Nature Communications
    10 (2019) 4113.
date_created: 2019-09-22T22:00:37Z
date_published: 2019-09-11T00:00:00Z
date_updated: 2023-08-30T06:21:23Z
day: '11'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1038/s41467-019-12068-x
external_id:
  isi:
  - '000485216800009'
  pmid:
  - '31511517'
file:
- access_level: open_access
  checksum: 62c2512712e16d27c1797d318d14ba9f
  content_type: application/pdf
  creator: kschuh
  date_created: 2019-10-01T11:18:50Z
  date_updated: 2020-07-14T12:47:44Z
  file_id: '6926'
  file_name: 2019_Nature_Bornhorst.pdf
  file_size: 3905793
  relation: main_file
file_date_updated: 2020-07-14T12:47:44Z
has_accepted_license: '1'
intvolume: '        10'
isi: 1
issue: '1'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
page: '4113'
pmid: 1
publication: Nature communications
publication_identifier:
  eissn:
  - '20411723'
publication_status: published
publisher: Nature Publishing Group
quality_controlled: '1'
scopus_import: '1'
status: public
title: Biomechanical signaling within the developing zebrafish heart attunes endocardial
  growth to myocardial chamber dimensions
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2019'
...