---
_id: '12880'
abstract:
- lang: eng
  text: Peripheral heterochromatin positioning depends on nuclear envelope associated
    proteins and repressive histone modifications. Here we show that overexpression
    (OE) of Lamin B1 (LmnB1) leads to the redistribution of peripheral heterochromatin
    into heterochromatic foci within the nucleoplasm. These changes represent a perturbation
    of heterochromatin binding at the nuclear periphery (NP) through a mechanism independent
    from altering other heterochromatin anchors or histone post-translational modifications.
    We further show that LmnB1 OE alters gene expression. These changes do not correlate
    with different levels of H3K9me3, but a significant number of the misregulated
    genes were likely mislocalized away from the NP upon LmnB1 OE. We also observed
    an enrichment of developmental processes amongst the upregulated genes. ~74% of
    these genes were normally repressed in our cell type, suggesting that LmnB1 OE
    promotes gene de-repression. This demonstrates a broader consequence of LmnB1
    OE on cell fate, and highlights the importance of maintaining proper levels of
    LmnB1.
acknowledgement: 'We thank members of the Hetzer lab for critical review of the manuscript;
  Novogene for mRNA library preparation and sequencing; the Next-Generation Sequencing
  Core Facility at the Salk Institute, with funding from NIH-NCI CCSG: P30 014195,
  the Chapman Foundation, and the Helmsley Charitable Trust, for sequencing Cut&Run
  libraries; and the Waitt Advanced Biophotonics Core Facility at the Salk Institute,
  with funding from NIH-NCI CCSG: P30 014195, the Waitt Foundation, and the Chan-Zuckerberg
  Initiative Imaging Scientist Award, for electron microscopy sample preparation and
  imaging.'
article_number: '2202548'
article_processing_charge: No
article_type: original
author:
- first_name: Jeanae M.
  full_name: Kaneshiro, Jeanae M.
  last_name: Kaneshiro
- first_name: Juliana S.
  full_name: Capitanio, Juliana S.
  last_name: Capitanio
- first_name: Martin W
  full_name: Hetzer, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: Hetzer
  orcid: 0000-0002-2111-992X
citation:
  ama: Kaneshiro JM, Capitanio JS, Hetzer M. Lamin B1 overexpression alters chromatin
    organization and gene expression. <i>Nucleus</i>. 2023;14(1). doi:<a href="https://doi.org/10.1080/19491034.2023.2202548">10.1080/19491034.2023.2202548</a>
  apa: Kaneshiro, J. M., Capitanio, J. S., &#38; Hetzer, M. (2023). Lamin B1 overexpression
    alters chromatin organization and gene expression. <i>Nucleus</i>. Taylor &#38;
    Francis. <a href="https://doi.org/10.1080/19491034.2023.2202548">https://doi.org/10.1080/19491034.2023.2202548</a>
  chicago: Kaneshiro, Jeanae M., Juliana S. Capitanio, and Martin Hetzer. “Lamin B1
    Overexpression Alters Chromatin Organization and Gene Expression.” <i>Nucleus</i>.
    Taylor &#38; Francis, 2023. <a href="https://doi.org/10.1080/19491034.2023.2202548">https://doi.org/10.1080/19491034.2023.2202548</a>.
  ieee: J. M. Kaneshiro, J. S. Capitanio, and M. Hetzer, “Lamin B1 overexpression
    alters chromatin organization and gene expression,” <i>Nucleus</i>, vol. 14, no.
    1. Taylor &#38; Francis, 2023.
  ista: Kaneshiro JM, Capitanio JS, Hetzer M. 2023. Lamin B1 overexpression alters
    chromatin organization and gene expression. Nucleus. 14(1), 2202548.
  mla: Kaneshiro, Jeanae M., et al. “Lamin B1 Overexpression Alters Chromatin Organization
    and Gene Expression.” <i>Nucleus</i>, vol. 14, no. 1, 2202548, Taylor &#38; Francis,
    2023, doi:<a href="https://doi.org/10.1080/19491034.2023.2202548">10.1080/19491034.2023.2202548</a>.
  short: J.M. Kaneshiro, J.S. Capitanio, M. Hetzer, Nucleus 14 (2023).
date_created: 2023-04-30T22:01:06Z
date_published: 2023-04-18T00:00:00Z
date_updated: 2023-08-01T14:18:46Z
day: '18'
ddc:
- '570'
department:
- _id: MaHe
doi: 10.1080/19491034.2023.2202548
external_id:
  isi:
  - '000971629400001'
  pmid:
  - '37071033'
file:
- access_level: open_access
  checksum: 8e707eda84f64dbad7f03545ae0a83ef
  content_type: application/pdf
  creator: dernst
  date_created: 2023-05-02T07:24:55Z
  date_updated: 2023-05-02T07:24:55Z
  file_id: '12884'
  file_name: 2023_Nucleus_Kaneshiro.pdf
  file_size: 3811113
  relation: main_file
  success: 1
file_date_updated: 2023-05-02T07:24:55Z
has_accepted_license: '1'
intvolume: '        14'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Nucleus
publication_identifier:
  eissn:
  - 1949-1042
  issn:
  - 1949-1034
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
scopus_import: '1'
status: public
title: Lamin B1 overexpression alters chromatin organization and gene expression
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 14
year: '2023'
...
---
_id: '11091'
abstract:
- lang: eng
  text: Neoplastic cells are often characterized by specific morphological abnormalities
    of the nuclear envelope (NE), which have been used for cancer diagnosis for more
    than a century. The NE is a double phospholipid bilayer that encapsulates the
    nuclear genome, regulates all nuclear trafficking of RNAs and proteins and prevents
    the passive diffusion of macromolecules between the nucleoplasm and the cytoplasm.
    Whether there is a consequence to the proper functioning of the cell and loss
    of structural integrity of the nucleus remains unclear. Using live cell imaging,
    we characterize a phenomenon wherein nuclei of several proliferating human cancer
    cell lines become temporarily ruptured during interphase. Strikingly, NE rupturing
    was associated with the mislocalization of nucleoplasmic and cytoplasmic proteins
    and, in the most extreme cases, the entrapment of cytoplasmic organelles in the
    nuclear interior. In addition, we observed the formation of micronuclei-like structures
    during interphase and the movement of chromatin out of the nuclear space. The
    frequency of these NE rupturing events was higher in cells in which the nuclear
    lamina, a network of intermediate filaments providing mechanical support to the
    NE, was not properly formed. Our data uncover the existence of a NE instability
    that has the potential to change the genomic landscape of cancer cells.
article_processing_charge: No
article_type: original
author:
- first_name: Jesse D.
  full_name: Vargas, Jesse D.
  last_name: Vargas
- first_name: Emily M.
  full_name: Hatch, Emily M.
  last_name: Hatch
- first_name: Daniel J.
  full_name: Anderson, Daniel J.
  last_name: Anderson
- first_name: Martin W
  full_name: HETZER, Martin W
  id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
  last_name: HETZER
  orcid: 0000-0002-2111-992X
citation:
  ama: Vargas JD, Hatch EM, Anderson DJ, Hetzer M. Transient nuclear envelope rupturing
    during interphase in human cancer cells. <i>Nucleus</i>. 2012;3(1):88-100. doi:<a
    href="https://doi.org/10.4161/nucl.18954">10.4161/nucl.18954</a>
  apa: Vargas, J. D., Hatch, E. M., Anderson, D. J., &#38; Hetzer, M. (2012). Transient
    nuclear envelope rupturing during interphase in human cancer cells. <i>Nucleus</i>.
    Taylor &#38; Francis. <a href="https://doi.org/10.4161/nucl.18954">https://doi.org/10.4161/nucl.18954</a>
  chicago: Vargas, Jesse D., Emily M. Hatch, Daniel J. Anderson, and Martin Hetzer.
    “Transient Nuclear Envelope Rupturing during Interphase in Human Cancer Cells.”
    <i>Nucleus</i>. Taylor &#38; Francis, 2012. <a href="https://doi.org/10.4161/nucl.18954">https://doi.org/10.4161/nucl.18954</a>.
  ieee: J. D. Vargas, E. M. Hatch, D. J. Anderson, and M. Hetzer, “Transient nuclear
    envelope rupturing during interphase in human cancer cells,” <i>Nucleus</i>, vol.
    3, no. 1. Taylor &#38; Francis, pp. 88–100, 2012.
  ista: Vargas JD, Hatch EM, Anderson DJ, Hetzer M. 2012. Transient nuclear envelope
    rupturing during interphase in human cancer cells. Nucleus. 3(1), 88–100.
  mla: Vargas, Jesse D., et al. “Transient Nuclear Envelope Rupturing during Interphase
    in Human Cancer Cells.” <i>Nucleus</i>, vol. 3, no. 1, Taylor &#38; Francis, 2012,
    pp. 88–100, doi:<a href="https://doi.org/10.4161/nucl.18954">10.4161/nucl.18954</a>.
  short: J.D. Vargas, E.M. Hatch, D.J. Anderson, M. Hetzer, Nucleus 3 (2012) 88–100.
date_created: 2022-04-07T07:51:53Z
date_published: 2012-01-01T00:00:00Z
date_updated: 2022-07-18T08:52:53Z
day: '01'
doi: 10.4161/nucl.18954
extern: '1'
external_id:
  pmid:
  - '22567193'
intvolume: '         3'
issue: '1'
keyword:
- Cell Biology
language:
- iso: eng
month: '01'
oa_version: None
page: 88-100
pmid: 1
publication: Nucleus
publication_identifier:
  eissn:
  - 1949-1042
  issn:
  - 1949-1034
publication_status: published
publisher: Taylor & Francis
quality_controlled: '1'
scopus_import: '1'
status: public
title: Transient nuclear envelope rupturing during interphase in human cancer cells
type: journal_article
user_id: 72615eeb-f1f3-11ec-aa25-d4573ddc34fd
volume: 3
year: '2012'
...