---
_id: '9822'
abstract:
- lang: eng
  text: Attachment of adhesive molecules on cell culture surfaces to restrict cell
    adhesion to defined areas and shapes has been vital for the progress of in vitro
    research. In currently existing patterning methods, a combination of pattern properties
    such as stability, precision, specificity, high-throughput outcome, and spatiotemporal
    control is highly desirable but challenging to achieve. Here, we introduce a versatile
    and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent
    patterning step and a subsequent functionalization of the pattern via click chemistry.
    This two-step process is feasible on arbitrary surfaces and allows for generation
    of sustainable patterns and gradients. The method is validated in different biological
    systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining
    the growth and migration of cells to the designated areas. We then implement a
    sequential photopatterning approach by adding a second switchable patterning step,
    allowing for spatiotemporal control over two distinct surface patterns. As a proof
    of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis.
    Our results show that the spatiotemporal control provided by our “sequential photopatterning”
    system is essential for mimicking dynamic biological processes and that our innovative
    approach has great potential for further applications in cell science.
acknowledgement: We would like to thank Charlott Leu for the production of our chromium
  wafers, Louise Ritter for her contribution of the IF stainings in Figure 4, Shokoufeh
  Teymouri for her help with the Bioinert coated slides, and finally Prof. Dr. Joachim
  Rädler for his valuable scientific guidance.
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Themistoklis
  full_name: Zisis, Themistoklis
  last_name: Zisis
- first_name: Jan
  full_name: Schwarz, Jan
  id: 346C1EC6-F248-11E8-B48F-1D18A9856A87
  last_name: Schwarz
- first_name: Miriam
  full_name: Balles, Miriam
  last_name: Balles
- first_name: Maibritt
  full_name: Kretschmer, Maibritt
  last_name: Kretschmer
- first_name: Maria
  full_name: Nemethova, Maria
  id: 34E27F1C-F248-11E8-B48F-1D18A9856A87
  last_name: Nemethova
- first_name: Remy P
  full_name: Chait, Remy P
  id: 3464AE84-F248-11E8-B48F-1D18A9856A87
  last_name: Chait
  orcid: 0000-0003-0876-3187
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Janina
  full_name: Lange, Janina
  last_name: Lange
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-4561-241X
- first_name: Stefan
  full_name: Zahler, Stefan
  last_name: Zahler
citation:
  ama: Zisis T, Schwarz J, Balles M, et al. Sequential and switchable patterning for
    studying cellular processes under spatiotemporal control. <i>ACS Applied Materials
    and Interfaces</i>. 2021;13(30):35545–35560. doi:<a href="https://doi.org/10.1021/acsami.1c09850">10.1021/acsami.1c09850</a>
  apa: Zisis, T., Schwarz, J., Balles, M., Kretschmer, M., Nemethova, M., Chait, R.
    P., … Zahler, S. (2021). Sequential and switchable patterning for studying cellular
    processes under spatiotemporal control. <i>ACS Applied Materials and Interfaces</i>.
    American Chemical Society. <a href="https://doi.org/10.1021/acsami.1c09850">https://doi.org/10.1021/acsami.1c09850</a>
  chicago: Zisis, Themistoklis, Jan Schwarz, Miriam Balles, Maibritt Kretschmer, Maria
    Nemethova, Remy P Chait, Robert Hauschild, et al. “Sequential and Switchable Patterning
    for Studying Cellular Processes under Spatiotemporal Control.” <i>ACS Applied
    Materials and Interfaces</i>. American Chemical Society, 2021. <a href="https://doi.org/10.1021/acsami.1c09850">https://doi.org/10.1021/acsami.1c09850</a>.
  ieee: T. Zisis <i>et al.</i>, “Sequential and switchable patterning for studying
    cellular processes under spatiotemporal control,” <i>ACS Applied Materials and
    Interfaces</i>, vol. 13, no. 30. American Chemical Society, pp. 35545–35560, 2021.
  ista: Zisis T, Schwarz J, Balles M, Kretschmer M, Nemethova M, Chait RP, Hauschild
    R, Lange J, Guet CC, Sixt MK, Zahler S. 2021. Sequential and switchable patterning
    for studying cellular processes under spatiotemporal control. ACS Applied Materials
    and Interfaces. 13(30), 35545–35560.
  mla: Zisis, Themistoklis, et al. “Sequential and Switchable Patterning for Studying
    Cellular Processes under Spatiotemporal Control.” <i>ACS Applied Materials and
    Interfaces</i>, vol. 13, no. 30, American Chemical Society, 2021, pp. 35545–35560,
    doi:<a href="https://doi.org/10.1021/acsami.1c09850">10.1021/acsami.1c09850</a>.
  short: T. Zisis, J. Schwarz, M. Balles, M. Kretschmer, M. Nemethova, R.P. Chait,
    R. Hauschild, J. Lange, C.C. Guet, M.K. Sixt, S. Zahler, ACS Applied Materials
    and Interfaces 13 (2021) 35545–35560.
date_created: 2021-08-08T22:01:28Z
date_published: 2021-08-04T00:00:00Z
date_updated: 2023-08-10T14:22:48Z
day: '04'
ddc:
- '620'
- '570'
department:
- _id: MiSi
- _id: GaTk
- _id: Bio
- _id: CaGu
doi: 10.1021/acsami.1c09850
ec_funded: 1
external_id:
  isi:
  - '000683741400026'
  pmid:
  - '34283577'
file:
- access_level: open_access
  checksum: b043a91d9f9200e467b970b692687ed3
  content_type: application/pdf
  creator: asandaue
  date_created: 2021-08-09T09:44:03Z
  date_updated: 2021-08-09T09:44:03Z
  file_id: '9833'
  file_name: 2021_ACSAppliedMaterialsAndInterfaces_Zisis.pdf
  file_size: 7123293
  relation: main_file
  success: 1
file_date_updated: 2021-08-09T09:44:03Z
has_accepted_license: '1'
intvolume: '        13'
isi: 1
issue: '30'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 35545–35560
pmid: 1
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
publication: ACS Applied Materials and Interfaces
publication_identifier:
  eissn:
  - '19448252'
  issn:
  - '19448244'
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Sequential and switchable patterning for studying cellular processes under
  spatiotemporal control
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2021'
...
---
_id: '8039'
abstract:
- lang: eng
  text: In the present work, we report a solution-based strategy to produce crystallographically
    textured SnSe bulk nanomaterials and printed layers with optimized thermoelectric
    performance in the direction normal to the substrate. Our strategy is based on
    the formulation of a molecular precursor that can be continuously decomposed to
    produce a SnSe powder or printed into predefined patterns. The precursor formulation
    and decomposition conditions are optimized to produce pure phase 2D SnSe nanoplates.
    The printed layer and the bulk material obtained after hot press displays a clear
    preferential orientation of the crystallographic domains, resulting in an ultralow
    thermal conductivity of 0.55 W m–1 K–1 in the direction normal to the substrate.
    Such textured nanomaterials present highly anisotropic properties with the best
    thermoelectric performance in plane, i.e., in the directions parallel to the substrate,
    which coincide with the crystallographic bc plane of SnSe. This is an unfortunate
    characteristic because thermoelectric devices are designed to create/harvest temperature
    gradients in the direction normal to the substrate. We further demonstrate that
    this limitation can be overcome with the introduction of small amounts of tellurium
    in the precursor. The presence of tellurium allows one to reduce the band gap
    and increase both the charge carrier concentration and the mobility, especially
    the cross plane, with a minimal decrease of the Seebeck coefficient. These effects
    translate into record out of plane ZT values at 800 K.
article_processing_charge: No
article_type: original
author:
- first_name: Yu
  full_name: Zhang, Yu
  last_name: Zhang
- first_name: Yu
  full_name: Liu, Yu
  id: 2A70014E-F248-11E8-B48F-1D18A9856A87
  last_name: Liu
  orcid: 0000-0001-7313-6740
- first_name: Congcong
  full_name: Xing, Congcong
  last_name: Xing
- first_name: Ting
  full_name: Zhang, Ting
  last_name: Zhang
- first_name: Mengyao
  full_name: Li, Mengyao
  last_name: Li
- first_name: Mercè
  full_name: Pacios, Mercè
  last_name: Pacios
- first_name: Xiaoting
  full_name: Yu, Xiaoting
  last_name: Yu
- first_name: Jordi
  full_name: Arbiol, Jordi
  last_name: Arbiol
- first_name: Jordi
  full_name: Llorca, Jordi
  last_name: Llorca
- first_name: Doris
  full_name: Cadavid, Doris
  last_name: Cadavid
- first_name: Maria
  full_name: Ibáñez, Maria
  id: 43C61214-F248-11E8-B48F-1D18A9856A87
  last_name: Ibáñez
  orcid: 0000-0001-5013-2843
- first_name: Andreu
  full_name: Cabot, Andreu
  last_name: Cabot
citation:
  ama: Zhang Y, Liu Y, Xing C, et al. Tin selenide molecular precursor for the solution
    processing of thermoelectric materials and devices. <i>ACS Applied Materials and
    Interfaces</i>. 2020;12(24):27104-27111. doi:<a href="https://doi.org/10.1021/acsami.0c04331">10.1021/acsami.0c04331</a>
  apa: Zhang, Y., Liu, Y., Xing, C., Zhang, T., Li, M., Pacios, M., … Cabot, A. (2020).
    Tin selenide molecular precursor for the solution processing of thermoelectric
    materials and devices. <i>ACS Applied Materials and Interfaces</i>. American Chemical
    Society. <a href="https://doi.org/10.1021/acsami.0c04331">https://doi.org/10.1021/acsami.0c04331</a>
  chicago: Zhang, Yu, Yu Liu, Congcong Xing, Ting Zhang, Mengyao Li, Mercè Pacios,
    Xiaoting Yu, et al. “Tin Selenide Molecular Precursor for the Solution Processing
    of Thermoelectric Materials and Devices.” <i>ACS Applied Materials and Interfaces</i>.
    American Chemical Society, 2020. <a href="https://doi.org/10.1021/acsami.0c04331">https://doi.org/10.1021/acsami.0c04331</a>.
  ieee: Y. Zhang <i>et al.</i>, “Tin selenide molecular precursor for the solution
    processing of thermoelectric materials and devices,” <i>ACS Applied Materials
    and Interfaces</i>, vol. 12, no. 24. American Chemical Society, pp. 27104–27111,
    2020.
  ista: Zhang Y, Liu Y, Xing C, Zhang T, Li M, Pacios M, Yu X, Arbiol J, Llorca J,
    Cadavid D, Ibáñez M, Cabot A. 2020. Tin selenide molecular precursor for the solution
    processing of thermoelectric materials and devices. ACS Applied Materials and
    Interfaces. 12(24), 27104–27111.
  mla: Zhang, Yu, et al. “Tin Selenide Molecular Precursor for the Solution Processing
    of Thermoelectric Materials and Devices.” <i>ACS Applied Materials and Interfaces</i>,
    vol. 12, no. 24, American Chemical Society, 2020, pp. 27104–11, doi:<a href="https://doi.org/10.1021/acsami.0c04331">10.1021/acsami.0c04331</a>.
  short: Y. Zhang, Y. Liu, C. Xing, T. Zhang, M. Li, M. Pacios, X. Yu, J. Arbiol,
    J. Llorca, D. Cadavid, M. Ibáñez, A. Cabot, ACS Applied Materials and Interfaces
    12 (2020) 27104–27111.
date_created: 2020-06-29T07:59:35Z
date_published: 2020-06-17T00:00:00Z
date_updated: 2023-08-22T07:50:08Z
day: '17'
department:
- _id: MaIb
doi: 10.1021/acsami.0c04331
ec_funded: 1
external_id:
  isi:
  - '000542925300032'
  pmid:
  - '32437128'
intvolume: '        12'
isi: 1
issue: '24'
language:
- iso: eng
month: '06'
oa_version: None
page: 27104-27111
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '754411'
  name: ISTplus - Postdoctoral Fellowships
publication: ACS Applied Materials and Interfaces
publication_identifier:
  eissn:
  - '19448252'
publication_status: published
publisher: American Chemical Society
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tin selenide molecular precursor for the solution processing of thermoelectric
  materials and devices
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2020'
...