---
_id: '8173'
abstract:
- lang: eng
  text: Understanding how the activity of membrane receptors and cellular signaling
    pathways shapes cell behavior is of fundamental interest in basic and applied
    research. Reengineering receptors to react to light instead of their cognate ligands
    allows for generating defined signaling inputs with high spatial and temporal
    precision and facilitates the dissection of complex signaling networks. Here,
    we describe fundamental considerations in the design of light-regulated receptor
    tyrosine kinases (Opto-RTKs) and appropriate control experiments. We also introduce
    methods for transient receptor expression in HEK293 cells, quantitative assessment
    of signaling activity in reporter gene assays, semiquantitative assessment of
    (in)activation time courses through Western blot (WB) analysis, and easy to implement
    light stimulation hardware.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Stephanie
  full_name: Kainrath, Stephanie
  id: 32CFBA64-F248-11E8-B48F-1D18A9856A87
  last_name: Kainrath
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: 'Kainrath S, Janovjak HL. Design and application of light-regulated receptor
    tyrosine kinases. In: Niopek D, ed. <i>Photoswitching Proteins</i>. Vol 2173.
    MIMB. Springer Nature; 2020:233-246. doi:<a href="https://doi.org/10.1007/978-1-0716-0755-8_16">10.1007/978-1-0716-0755-8_16</a>'
  apa: Kainrath, S., &#38; Janovjak, H. L. (2020). Design and application of light-regulated
    receptor tyrosine kinases. In D. Niopek (Ed.), <i>Photoswitching Proteins</i>
    (Vol. 2173, pp. 233–246). Springer Nature. <a href="https://doi.org/10.1007/978-1-0716-0755-8_16">https://doi.org/10.1007/978-1-0716-0755-8_16</a>
  chicago: Kainrath, Stephanie, and Harald L Janovjak. “Design and Application of
    Light-Regulated Receptor Tyrosine Kinases.” In <i>Photoswitching Proteins</i>,
    edited by Dominik Niopek, 2173:233–46. MIMB. Springer Nature, 2020. <a href="https://doi.org/10.1007/978-1-0716-0755-8_16">https://doi.org/10.1007/978-1-0716-0755-8_16</a>.
  ieee: S. Kainrath and H. L. Janovjak, “Design and application of light-regulated
    receptor tyrosine kinases,” in <i>Photoswitching Proteins</i>, vol. 2173, D. Niopek,
    Ed. Springer Nature, 2020, pp. 233–246.
  ista: 'Kainrath S, Janovjak HL. 2020.Design and application of light-regulated receptor
    tyrosine kinases. In: Photoswitching Proteins. Methods in Molecular Biology, vol.
    2173, 233–246.'
  mla: Kainrath, Stephanie, and Harald L. Janovjak. “Design and Application of Light-Regulated
    Receptor Tyrosine Kinases.” <i>Photoswitching Proteins</i>, edited by Dominik
    Niopek, vol. 2173, Springer Nature, 2020, pp. 233–46, doi:<a href="https://doi.org/10.1007/978-1-0716-0755-8_16">10.1007/978-1-0716-0755-8_16</a>.
  short: S. Kainrath, H.L. Janovjak, in:, D. Niopek (Ed.), Photoswitching Proteins,
    Springer Nature, 2020, pp. 233–246.
date_created: 2020-07-26T22:01:03Z
date_published: 2020-07-11T00:00:00Z
date_updated: 2021-01-12T08:17:17Z
day: '11'
department:
- _id: CaGu
doi: 10.1007/978-1-0716-0755-8_16
editor:
- first_name: Dominik
  full_name: Niopek, Dominik
  last_name: Niopek
intvolume: '      2173'
language:
- iso: eng
month: '07'
oa_version: None
page: 233-246
publication: Photoswitching Proteins
publication_identifier:
  eisbn:
  - '9781071607558'
  eissn:
  - '19406029'
publication_status: published
publisher: Springer Nature
scopus_import: '1'
series_title: MIMB
status: public
title: Design and application of light-regulated receptor tyrosine kinases
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2173
year: '2020'
...