---
_id: '14299'
abstract:
- lang: eng
  text: DNA origami nano-objects are usually designed around generic single-stranded
    “scaffolds”. Many properties of the target object are determined by details of
    those generic scaffold sequences. Here, we enable designers to fully specify the
    target structure not only in terms of desired 3D shape but also in terms of the
    sequences used. To this end, we built design tools to construct scaffold sequences
    de novo based on strand diagrams, and we developed scalable production methods
    for creating design-specific scaffold strands with fully user-defined sequences.
    We used 17 custom scaffolds having different lengths and sequence properties to
    study the influence of sequence redundancy and sequence composition on multilayer
    DNA origami assembly and to realize efficient one-pot assembly of multiscaffold
    DNA origami objects. Furthermore, as examples for functionalized scaffolds, we
    created a scaffold that enables direct, covalent cross-linking of DNA origami
    via UV irradiation, and we built DNAzyme-containing scaffolds that allow postfolding
    DNA origami domain separation.
article_processing_charge: No
article_type: original
author:
- first_name: Engelhardt
  full_name: FAS, Engelhardt
  last_name: FAS
- first_name: Florian M
  full_name: Praetorius, Florian M
  id: dfec9381-4341-11ee-8fd8-faa02bba7d62
  last_name: Praetorius
- first_name: CH
  full_name: Wachauf, CH
  last_name: Wachauf
- first_name: G
  full_name: Brüggenthies, G
  last_name: Brüggenthies
- first_name: F
  full_name: Kohler, F
  last_name: Kohler
- first_name: B
  full_name: Kick, B
  last_name: Kick
- first_name: KL
  full_name: Kadletz, KL
  last_name: Kadletz
- first_name: PN
  full_name: Pham, PN
  last_name: Pham
- first_name: KL
  full_name: Behler, KL
  last_name: Behler
- first_name: T
  full_name: Gerling, T
  last_name: Gerling
- first_name: H
  full_name: Dietz, H
  last_name: Dietz
citation:
  ama: FAS E, Praetorius FM, Wachauf C, et al. Custom-size, functional, and durable
    DNA origami with design-specific scaffolds. <i>ACS Nano</i>. 2019;13(5):5015-5027.
    doi:<a href="https://doi.org/10.1021/acsnano.9b01025">10.1021/acsnano.9b01025</a>
  apa: FAS, E., Praetorius, F. M., Wachauf, C., Brüggenthies, G., Kohler, F., Kick,
    B., … Dietz, H. (2019). Custom-size, functional, and durable DNA origami with
    design-specific scaffolds. <i>ACS Nano</i>. ACS Publications. <a href="https://doi.org/10.1021/acsnano.9b01025">https://doi.org/10.1021/acsnano.9b01025</a>
  chicago: FAS, Engelhardt, Florian M Praetorius, CH Wachauf, G Brüggenthies, F Kohler,
    B Kick, KL Kadletz, et al. “Custom-Size, Functional, and Durable DNA Origami with
    Design-Specific Scaffolds.” <i>ACS Nano</i>. ACS Publications, 2019. <a href="https://doi.org/10.1021/acsnano.9b01025">https://doi.org/10.1021/acsnano.9b01025</a>.
  ieee: E. FAS <i>et al.</i>, “Custom-size, functional, and durable DNA origami with
    design-specific scaffolds,” <i>ACS Nano</i>, vol. 13, no. 5. ACS Publications,
    pp. 5015–5027, 2019.
  ista: FAS E, Praetorius FM, Wachauf C, Brüggenthies G, Kohler F, Kick B, Kadletz
    K, Pham P, Behler K, Gerling T, Dietz H. 2019. Custom-size, functional, and durable
    DNA origami with design-specific scaffolds. ACS Nano. 13(5), 5015–5027.
  mla: FAS, Engelhardt, et al. “Custom-Size, Functional, and Durable DNA Origami with
    Design-Specific Scaffolds.” <i>ACS Nano</i>, vol. 13, no. 5, ACS Publications,
    2019, pp. 5015–27, doi:<a href="https://doi.org/10.1021/acsnano.9b01025">10.1021/acsnano.9b01025</a>.
  short: E. FAS, F.M. Praetorius, C. Wachauf, G. Brüggenthies, F. Kohler, B. Kick,
    K. Kadletz, P. Pham, K. Behler, T. Gerling, H. Dietz, ACS Nano 13 (2019) 5015–5027.
date_created: 2023-09-06T12:48:47Z
date_published: 2019-04-16T00:00:00Z
date_updated: 2023-11-07T12:17:31Z
day: '16'
doi: 10.1021/acsnano.9b01025
extern: '1'
external_id:
  pmid:
  - '30990672'
intvolume: '        13'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1021/acsnano.9b01025
month: '04'
oa: 1
oa_version: Published Version
page: 5015-5027
pmid: 1
publication: ACS Nano
publication_identifier:
  eissn:
  - 1936-086x
  issn:
  - 1936-0851
publication_status: published
publisher: ACS Publications
quality_controlled: '1'
scopus_import: '1'
status: public
title: Custom-size, functional, and durable DNA origami with design-specific scaffolds
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2019'
...