---
_id: '12163'
abstract:
- lang: eng
  text: Small GTPases play essential roles in the organization of eukaryotic cells.
    In recent years, it has become clear that their intracellular functions result
    from intricate biochemical networks of the GTPase and their regulators that dynamically
    bind to a membrane surface. Due to the inherent complexities of their interactions,
    however, revealing the underlying mechanisms of action is often difficult to achieve
    from in vivo studies. This review summarizes in vitro reconstitution approaches
    developed to obtain a better mechanistic understanding of how small GTPase activities
    are regulated in space and time.
acknowledgement: The authors acknowledge support from IST Austria and helpful comments
  from the anonymous reviewers that helped to improve this manuscript. We apologize
  to the authors of primary literature and outstanding research not cited here due
  to space restraints.
article_processing_charge: Yes (via OA deal)
article_type: review
author:
- first_name: Martin
  full_name: Loose, Martin
  id: 462D4284-F248-11E8-B48F-1D18A9856A87
  last_name: Loose
  orcid: 0000-0001-7309-9724
- first_name: Albert
  full_name: Auer, Albert
  id: 3018E8C2-F248-11E8-B48F-1D18A9856A87
  last_name: Auer
  orcid: 0000-0002-3580-2906
- first_name: Gabriel
  full_name: Brognara, Gabriel
  id: D96FFDA0-A884-11E9-9968-DC26E6697425
  last_name: Brognara
- first_name: Hanifatul R
  full_name: Budiman, Hanifatul R
  id: 55380f95-15b2-11ec-abd3-aff8e230696b
  last_name: Budiman
- first_name: Lukasz M
  full_name: Kowalski, Lukasz M
  id: e3a512e2-4bbe-11eb-a68a-e3857a7844c2
  last_name: Kowalski
- first_name: Ivana
  full_name: Matijevic, Ivana
  id: 83c17ce3-15b2-11ec-abd3-f486545870bd
  last_name: Matijevic
citation:
  ama: Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. In vitro
    reconstitution of small GTPase regulation. <i>FEBS Letters</i>. 2023;597(6):762-777.
    doi:<a href="https://doi.org/10.1002/1873-3468.14540">10.1002/1873-3468.14540</a>
  apa: Loose, M., Auer, A., Brognara, G., Budiman, H. R., Kowalski, L. M., &#38; Matijevic,
    I. (2023). In vitro reconstitution of small GTPase regulation. <i>FEBS Letters</i>.
    Wiley. <a href="https://doi.org/10.1002/1873-3468.14540">https://doi.org/10.1002/1873-3468.14540</a>
  chicago: Loose, Martin, Albert Auer, Gabriel Brognara, Hanifatul R Budiman, Lukasz
    M Kowalski, and Ivana Matijevic. “In Vitro Reconstitution of Small GTPase Regulation.”
    <i>FEBS Letters</i>. Wiley, 2023. <a href="https://doi.org/10.1002/1873-3468.14540">https://doi.org/10.1002/1873-3468.14540</a>.
  ieee: M. Loose, A. Auer, G. Brognara, H. R. Budiman, L. M. Kowalski, and I. Matijevic,
    “In vitro reconstitution of small GTPase regulation,” <i>FEBS Letters</i>, vol.
    597, no. 6. Wiley, pp. 762–777, 2023.
  ista: Loose M, Auer A, Brognara G, Budiman HR, Kowalski LM, Matijevic I. 2023. In
    vitro reconstitution of small GTPase regulation. FEBS Letters. 597(6), 762–777.
  mla: Loose, Martin, et al. “In Vitro Reconstitution of Small GTPase Regulation.”
    <i>FEBS Letters</i>, vol. 597, no. 6, Wiley, 2023, pp. 762–77, doi:<a href="https://doi.org/10.1002/1873-3468.14540">10.1002/1873-3468.14540</a>.
  short: M. Loose, A. Auer, G. Brognara, H.R. Budiman, L.M. Kowalski, I. Matijevic,
    FEBS Letters 597 (2023) 762–777.
date_created: 2023-01-12T12:09:58Z
date_published: 2023-03-01T00:00:00Z
date_updated: 2023-08-16T08:32:29Z
day: '01'
ddc:
- '570'
department:
- _id: MaLo
doi: 10.1002/1873-3468.14540
external_id:
  isi:
  - '000891573000001'
  pmid:
  - '36448231'
file:
- access_level: open_access
  checksum: 7492244d3f9c5faa1347ef03f6e5bc84
  content_type: application/pdf
  creator: dernst
  date_created: 2023-08-16T08:31:04Z
  date_updated: 2023-08-16T08:31:04Z
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  file_name: 2023_FEBSLetters_Loose.pdf
  file_size: 3148143
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file_date_updated: 2023-08-16T08:31:04Z
has_accepted_license: '1'
intvolume: '       597'
isi: 1
issue: '6'
keyword:
- Cell Biology
- Genetics
- Molecular Biology
- Biochemistry
- Structural Biology
- Biophysics
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
page: 762-777
pmid: 1
publication: FEBS Letters
publication_identifier:
  eissn:
  - 1873-3468
  issn:
  - 0014-5793
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro reconstitution of small GTPase regulation
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 597
year: '2023'
...