---
_id: '8274'
abstract:
- lang: eng
  text: 'Background/Aim: Our aim was to investigate the crosstalk between tumor and
    immune cells (M2 macrophages) and its effects on cyclo-oxygenase-2 (COX2) regulation
    in canine mammary tumors (CMT). Materials and Methods: Sh1b CMT cells and human
    BT474 mammary or HT29 colon cancer cells were co-cultured with canine peripheral
    blood mononuclear cells (PBMCs) or with macrophage-like differentiated THP1 monocytes
    (dTHP1). Intracellular COX2 expression by PBMCs, dTHP1 and cancer cells was evaluated
    by flow cytometry. Results: Co-culturing of Sh1b and canine PBMCs induced COX2
    overexpression in CMT cells. In turn, COX2 expression by PBMCs, mostly CD68+ macrophages,
    was attenuated by co-culture with Sh1b (p=0.0001). In accordance, co-culture with
    dTHP1 prompted intracellular production of COX2 in both Sh1b CMT cells and HT29
    human colon cancer cells and reduced production of COX2 in BT474 human mammary
    cancer cells. The intracellular COX2 expression from dTHP1 decreased when treated
    with conditioned medium from cultured Sh1b and HT29 cancer cells. Conclusion:
    Bidirectional COX2 regulation between cancer and monocytes/macrophages might shape
    a tolerogenic tumor microenvironment in CMT.'
article_processing_charge: No
article_type: original
author:
- first_name: Maria Isabel
  full_name: Carvalho, Maria Isabel
  last_name: Carvalho
- first_name: Rodolfo
  full_name: Bianchini, Rodolfo
  last_name: Bianchini
- first_name: Judit
  full_name: Fazekas-Singer, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Fazekas-Singer
  orcid: 0000-0002-8777-3502
- first_name: Ina
  full_name: Herrmann, Ina
  last_name: Herrmann
- first_name: Irene
  full_name: Flickinger, Irene
  last_name: Flickinger
- first_name: Johann G.
  full_name: Thalhammer, Johann G.
  last_name: Thalhammer
- first_name: Isabel
  full_name: Pires, Isabel
  last_name: Pires
- first_name: Erika
  full_name: Jensen-Jarolim, Erika
  last_name: Jensen-Jarolim
- first_name: Felisbina L.
  full_name: Queiroga, Felisbina L.
  last_name: Queiroga
citation:
  ama: Carvalho MI, Bianchini R, Singer J, et al. Bidirectional regulation of COX-2
    expression between cancer cells and macrophages. <i>Anticancer Research</i>. 2018;38(5):2811-2817.
    doi:<a href="https://doi.org/10.21873/anticanres.12525">10.21873/anticanres.12525</a>
  apa: Carvalho, M. I., Bianchini, R., Singer, J., Herrmann, I., Flickinger, I., Thalhammer,
    J. G., … Queiroga, F. L. (2018). Bidirectional regulation of COX-2 expression
    between cancer cells and macrophages. <i>Anticancer Research</i>. International
    Institute of Anticancer Research. <a href="https://doi.org/10.21873/anticanres.12525">https://doi.org/10.21873/anticanres.12525</a>
  chicago: Carvalho, Maria Isabel, Rodolfo Bianchini, Judit Singer, Ina Herrmann,
    Irene Flickinger, Johann G. Thalhammer, Isabel Pires, Erika Jensen-Jarolim, and
    Felisbina L. Queiroga. “Bidirectional Regulation of COX-2 Expression between Cancer
    Cells and Macrophages.” <i>Anticancer Research</i>. International Institute of
    Anticancer Research, 2018. <a href="https://doi.org/10.21873/anticanres.12525">https://doi.org/10.21873/anticanres.12525</a>.
  ieee: M. I. Carvalho <i>et al.</i>, “Bidirectional regulation of COX-2 expression
    between cancer cells and macrophages,” <i>Anticancer Research</i>, vol. 38, no.
    5. International Institute of Anticancer Research, pp. 2811–2817, 2018.
  ista: Carvalho MI, Bianchini R, Singer J, Herrmann I, Flickinger I, Thalhammer JG,
    Pires I, Jensen-Jarolim E, Queiroga FL. 2018. Bidirectional regulation of COX-2
    expression between cancer cells and macrophages. Anticancer Research. 38(5), 2811–2817.
  mla: Carvalho, Maria Isabel, et al. “Bidirectional Regulation of COX-2 Expression
    between Cancer Cells and Macrophages.” <i>Anticancer Research</i>, vol. 38, no.
    5, International Institute of Anticancer Research, 2018, pp. 2811–17, doi:<a href="https://doi.org/10.21873/anticanres.12525">10.21873/anticanres.12525</a>.
  short: M.I. Carvalho, R. Bianchini, J. Singer, I. Herrmann, I. Flickinger, J.G.
    Thalhammer, I. Pires, E. Jensen-Jarolim, F.L. Queiroga, Anticancer Research 38
    (2018) 2811–2817.
date_created: 2020-08-17T07:13:55Z
date_published: 2018-05-01T00:00:00Z
date_updated: 2021-01-12T08:17:52Z
day: '01'
doi: 10.21873/anticanres.12525
extern: '1'
intvolume: '        38'
issue: '5'
language:
- iso: eng
month: '05'
oa_version: None
page: 2811-2817
publication: Anticancer Research
publication_identifier:
  eissn:
  - 1791-7530
  issn:
  - 0250-7005
publication_status: published
publisher: International Institute of Anticancer Research
quality_controlled: '1'
status: public
title: Bidirectional regulation of COX-2 expression between cancer cells and macrophages
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 38
year: '2018'
...