---
_id: '9530'
abstract:
- lang: eng
  text: "Background\r\nDNA methylation of active genes, also known as gene body methylation,
    is found in many animal and plant genomes. Despite this, the transcriptional and
    developmental role of such methylation remains poorly understood. Here, we explore
    the dynamic range of DNA methylation in honey bee, a model organism for gene body
    methylation.\r\n\r\nResults\r\nOur data show that CG methylation in gene bodies
    globally fluctuates during honey bee development. However, these changes cause
    no gene expression alterations. Intriguingly, despite the global alterations,
    tissue-specific CG methylation patterns of complete genes or exons are rare, implying
    robust maintenance of genic methylation during development. Additionally, we show
    that CG methylation maintenance fluctuates in somatic cells, while reaching maximum
    fidelity in sperm cells. Finally, unlike universally present CG methylation, we
    discovered non-CG methylation specifically in bee heads that resembles such methylation
    in mammalian brain tissue.\r\n\r\nConclusions\r\nBased on these results, we propose
    that gene body CG methylation can oscillate during development if it is kept to
    a level adequate to preserve function. Additionally, our data suggest that heightened
    non-CG methylation is a conserved regulator of animal nervous systems."
article_number: '62'
article_processing_charge: No
article_type: original
author:
- first_name: Keith D.
  full_name: Harris, Keith D.
  last_name: Harris
- first_name: James P. B.
  full_name: Lloyd, James P. B.
  last_name: Lloyd
- first_name: Katherine
  full_name: Domb, Katherine
  last_name: Domb
- first_name: Daniel
  full_name: Zilberman, Daniel
  id: 6973db13-dd5f-11ea-814e-b3e5455e9ed1
  last_name: Zilberman
  orcid: 0000-0002-0123-8649
- first_name: Assaf
  full_name: Zemach, Assaf
  last_name: Zemach
citation:
  ama: Harris KD, Lloyd JPB, Domb K, Zilberman D, Zemach A. DNA methylation is maintained
    with high fidelity in the honey bee germline and exhibits global non-functional
    fluctuations during somatic development. <i>Epigenetics and Chromatin</i>. 2019;12.
    doi:<a href="https://doi.org/10.1186/s13072-019-0307-4">10.1186/s13072-019-0307-4</a>
  apa: Harris, K. D., Lloyd, J. P. B., Domb, K., Zilberman, D., &#38; Zemach, A. (2019).
    DNA methylation is maintained with high fidelity in the honey bee germline and
    exhibits global non-functional fluctuations during somatic development. <i>Epigenetics
    and Chromatin</i>. Springer Nature. <a href="https://doi.org/10.1186/s13072-019-0307-4">https://doi.org/10.1186/s13072-019-0307-4</a>
  chicago: Harris, Keith D., James P. B. Lloyd, Katherine Domb, Daniel Zilberman,
    and Assaf Zemach. “DNA Methylation Is Maintained with High Fidelity in the Honey
    Bee Germline and Exhibits Global Non-Functional Fluctuations during Somatic Development.”
    <i>Epigenetics and Chromatin</i>. Springer Nature, 2019. <a href="https://doi.org/10.1186/s13072-019-0307-4">https://doi.org/10.1186/s13072-019-0307-4</a>.
  ieee: K. D. Harris, J. P. B. Lloyd, K. Domb, D. Zilberman, and A. Zemach, “DNA methylation
    is maintained with high fidelity in the honey bee germline and exhibits global
    non-functional fluctuations during somatic development,” <i>Epigenetics and Chromatin</i>,
    vol. 12. Springer Nature, 2019.
  ista: Harris KD, Lloyd JPB, Domb K, Zilberman D, Zemach A. 2019. DNA methylation
    is maintained with high fidelity in the honey bee germline and exhibits global
    non-functional fluctuations during somatic development. Epigenetics and Chromatin.
    12, 62.
  mla: Harris, Keith D., et al. “DNA Methylation Is Maintained with High Fidelity
    in the Honey Bee Germline and Exhibits Global Non-Functional Fluctuations during
    Somatic Development.” <i>Epigenetics and Chromatin</i>, vol. 12, 62, Springer
    Nature, 2019, doi:<a href="https://doi.org/10.1186/s13072-019-0307-4">10.1186/s13072-019-0307-4</a>.
  short: K.D. Harris, J.P.B. Lloyd, K. Domb, D. Zilberman, A. Zemach, Epigenetics
    and Chromatin 12 (2019).
date_created: 2021-06-08T09:21:51Z
date_published: 2019-10-10T00:00:00Z
date_updated: 2021-12-14T07:53:00Z
day: '10'
ddc:
- '570'
department:
- _id: DaZi
doi: 10.1186/s13072-019-0307-4
extern: '1'
external_id:
  pmid:
  - '31601251'
file:
- access_level: open_access
  checksum: 86ff50a7517891511af2733c76c81b67
  content_type: application/pdf
  creator: asandaue
  date_created: 2021-06-08T09:29:19Z
  date_updated: 2021-06-08T09:29:19Z
  file_id: '9531'
  file_name: 2019_EpigeneticsAndChromatin_Harris.pdf
  file_size: 3221067
  relation: main_file
  success: 1
file_date_updated: 2021-06-08T09:29:19Z
has_accepted_license: '1'
intvolume: '        12'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: Epigenetics and Chromatin
publication_identifier:
  eissn:
  - 1756-8935
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: DNA methylation is maintained with high fidelity in the honey bee germline
  and exhibits global non-functional fluctuations during somatic development
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 8b945eb4-e2f2-11eb-945a-df72226e66a9
volume: 12
year: '2019'
...