---
_id: '6819'
abstract:
- lang: eng
  text: Glyphosate (N-phosphonomethyl glycine) and its commercial herbicide formulations
    have been shown to exert toxicity via various mechanisms. It has been asserted
    that glyphosate substitutes for glycine in polypeptide chains leading to protein
    misfolding and toxicity. However, as no direct evidence exists for glycine to
    glyphosate substitution in proteins, including in mammalian organisms, we tested
    this claim by conducting a proteomics analysis of MDA-MB-231 human breast cancer
    cells grown in the presence of 100 mg/L glyphosate for 6 days. Protein extracts
    from three treated and three untreated cell cultures were analysed as one TMT-6plex
    labelled sample, to highlight a specific pattern (+/+/+/−/−/−) of reporter intensities
    for peptides bearing true glyphosate treatment induced-post translational modifications
    as well as allowing an investigation of the total proteome.
article_number: '494'
article_processing_charge: No
author:
- first_name: Michael N.
  full_name: Antoniou, Michael N.
  last_name: Antoniou
- first_name: Armel
  full_name: Nicolas, Armel
  id: 2A103192-F248-11E8-B48F-1D18A9856A87
  last_name: Nicolas
- first_name: Robin
  full_name: Mesnage, Robin
  last_name: Mesnage
- first_name: Martina
  full_name: Biserni, Martina
  last_name: Biserni
- first_name: Francesco V.
  full_name: Rao, Francesco V.
  last_name: Rao
- first_name: Cristina Vazquez
  full_name: Martin, Cristina Vazquez
  last_name: Martin
citation:
  ama: Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. Glyphosate
    does not substitute for glycine in proteins of actively dividing mammalian cells.
    <i>BMC Research Notes</i>. 2019;12. doi:<a href="https://doi.org/10.1186/s13104-019-4534-3">10.1186/s13104-019-4534-3</a>
  apa: Antoniou, M. N., Nicolas, A., Mesnage, R., Biserni, M., Rao, F. V., &#38; Martin,
    C. V. (2019). Glyphosate does not substitute for glycine in proteins of actively
    dividing mammalian cells. <i>BMC Research Notes</i>. BioMed Central. <a href="https://doi.org/10.1186/s13104-019-4534-3">https://doi.org/10.1186/s13104-019-4534-3</a>
  chicago: Antoniou, Michael N., Armel Nicolas, Robin Mesnage, Martina Biserni, Francesco
    V. Rao, and Cristina Vazquez Martin. “Glyphosate Does Not Substitute for Glycine
    in Proteins of Actively Dividing Mammalian Cells.” <i>BMC Research Notes</i>.
    BioMed Central, 2019. <a href="https://doi.org/10.1186/s13104-019-4534-3">https://doi.org/10.1186/s13104-019-4534-3</a>.
  ieee: M. N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F. V. Rao, and C. V. Martin,
    “Glyphosate does not substitute for glycine in proteins of actively dividing mammalian
    cells,” <i>BMC Research Notes</i>, vol. 12. BioMed Central, 2019.
  ista: Antoniou MN, Nicolas A, Mesnage R, Biserni M, Rao FV, Martin CV. 2019. Glyphosate
    does not substitute for glycine in proteins of actively dividing mammalian cells.
    BMC Research Notes. 12, 494.
  mla: Antoniou, Michael N., et al. “Glyphosate Does Not Substitute for Glycine in
    Proteins of Actively Dividing Mammalian Cells.” <i>BMC Research Notes</i>, vol.
    12, 494, BioMed Central, 2019, doi:<a href="https://doi.org/10.1186/s13104-019-4534-3">10.1186/s13104-019-4534-3</a>.
  short: M.N. Antoniou, A. Nicolas, R. Mesnage, M. Biserni, F.V. Rao, C.V. Martin,
    BMC Research Notes 12 (2019).
date_created: 2019-08-18T22:00:39Z
date_published: 2019-08-08T00:00:00Z
date_updated: 2023-02-23T14:08:14Z
day: '08'
ddc:
- '570'
department:
- _id: LifeSc
doi: 10.1186/s13104-019-4534-3
external_id:
  pmid:
  - '31395095'
file:
- access_level: open_access
  checksum: 4a2bb7994b7f2c432bf44f5127ea3102
  content_type: application/pdf
  creator: dernst
  date_created: 2019-08-23T11:10:35Z
  date_updated: 2020-07-14T12:47:40Z
  file_id: '6829'
  file_name: 2019_BMC_Antoniou.pdf
  file_size: 1177482
  relation: main_file
file_date_updated: 2020-07-14T12:47:40Z
has_accepted_license: '1'
intvolume: '        12'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: BMC Research Notes
publication_identifier:
  eissn:
  - 1756-0500
publication_status: published
publisher: BioMed Central
quality_controlled: '1'
related_material:
  record:
  - id: '9784'
    relation: research_data
    status: public
scopus_import: 1
status: public
title: Glyphosate does not substitute for glycine in proteins of actively dividing
  mammalian cells
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 12
year: '2019'
...