---
_id: '7207'
abstract:
- lang: eng
  text: The hippocampus plays key roles in learning and memory and is a main target
    of Alzheimer's disease (AD), which causes progressive memory impairments. Despite
    numerous investigations about the processes required for the normal hippocampal
    functions, the neurotransmitter receptors involved in the synaptic deficits by
    which AD disables the hippocampus are not yet characterized. By combining histoblots,
    western blots, immunohistochemistry and high‐resolution immunoelectron microscopic
    methods for GABAB receptors, this study provides a quantitative description of
    the expression and the subcellular localization of GABAB1 in the hippocampus in
    a mouse model of AD at 1, 6 and 12 months of age. Western blots and histoblots
    showed that the total amount of protein and the laminar expression pattern of
    GABAB1 were similar in APP/PS1 mice and in age‐matched wild‐type mice. In contrast,
    immunoelectron microscopic techniques showed that the subcellular localization
    of GABAB1 subunit did not change significantly in APP/PS1 mice at 1 month of age,
    was significantly reduced in the stratum lacunosum‐moleculare of CA1 pyramidal
    cells at 6 months of age and significantly reduced at the membrane surface of
    CA1 pyramidal cells at 12 months of age. This reduction of plasma membrane GABAB1
    was paralleled by a significant increase of the subunit at the intracellular sites.
    We further observed a decrease of membrane‐targeted GABAB receptors in axon terminals
    contacting CA1 pyramidal cells. Our data demonstrate compartment‐ and age‐dependent
    reduction of plasma membrane‐targeted GABAB receptors in the CA1 region of the
    hippocampus, suggesting that this decrease might be enough to alter the GABAB‐mediated
    synaptic transmission taking place in AD.
article_processing_charge: No
article_type: original
author:
- first_name: Alejandro
  full_name: Martín-Belmonte, Alejandro
  last_name: Martín-Belmonte
- first_name: Carolina
  full_name: Aguado, Carolina
  last_name: Aguado
- first_name: Rocío
  full_name: Alfaro-Ruíz, Rocío
  last_name: Alfaro-Ruíz
- first_name: Ana Esther
  full_name: Moreno-Martínez, Ana Esther
  last_name: Moreno-Martínez
- first_name: Luis
  full_name: De La Ossa, Luis
  last_name: De La Ossa
- first_name: José
  full_name: Martínez-Hernández, José
  last_name: Martínez-Hernández
- first_name: Alain
  full_name: Buisson, Alain
  last_name: Buisson
- first_name: Simon
  full_name: Früh, Simon
  last_name: Früh
- first_name: Bernhard
  full_name: Bettler, Bernhard
  last_name: Bettler
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Yugo
  full_name: Fukazawa, Yugo
  last_name: Fukazawa
- first_name: Rafael
  full_name: Luján, Rafael
  last_name: Luján
citation:
  ama: Martín-Belmonte A, Aguado C, Alfaro-Ruíz R, et al. Reduction in the neuronal
    surface of post and presynaptic GABA>B< receptors in the hippocampus in
    a mouse model of Alzheimer’s disease. <i>Brain Pathology</i>. 2020;30(3):554-575.
    doi:<a href="https://doi.org/10.1111/bpa.12802">10.1111/bpa.12802</a>
  apa: Martín-Belmonte, A., Aguado, C., Alfaro-Ruíz, R., Moreno-Martínez, A. E., De
    La Ossa, L., Martínez-Hernández, J., … Luján, R. (2020). Reduction in the neuronal
    surface of post and presynaptic GABA&#62;B&#60; receptors in the hippocampus in
    a mouse model of Alzheimer’s disease. <i>Brain Pathology</i>. Wiley. <a href="https://doi.org/10.1111/bpa.12802">https://doi.org/10.1111/bpa.12802</a>
  chicago: Martín-Belmonte, Alejandro, Carolina Aguado, Rocío Alfaro-Ruíz, Ana Esther
    Moreno-Martínez, Luis De La Ossa, José Martínez-Hernández, Alain Buisson, et al.
    “Reduction in the Neuronal Surface of Post and Presynaptic GABA&#62;B&#60; Receptors
    in the Hippocampus in a Mouse Model of Alzheimer’s Disease.” <i>Brain Pathology</i>.
    Wiley, 2020. <a href="https://doi.org/10.1111/bpa.12802">https://doi.org/10.1111/bpa.12802</a>.
  ieee: A. Martín-Belmonte <i>et al.</i>, “Reduction in the neuronal surface of post
    and presynaptic GABA&#62;B&#60; receptors in the hippocampus in a mouse model
    of Alzheimer’s disease,” <i>Brain Pathology</i>, vol. 30, no. 3. Wiley, pp. 554–575,
    2020.
  ista: Martín-Belmonte A, Aguado C, Alfaro-Ruíz R, Moreno-Martínez AE, De La Ossa
    L, Martínez-Hernández J, Buisson A, Früh S, Bettler B, Shigemoto R, Fukazawa Y,
    Luján R. 2020. Reduction in the neuronal surface of post and presynaptic GABA&#62;B&#60;
    receptors in the hippocampus in a mouse model of Alzheimer’s disease. Brain Pathology.
    30(3), 554–575.
  mla: Martín-Belmonte, Alejandro, et al. “Reduction in the Neuronal Surface of Post
    and Presynaptic GABA&#62;B&#60; Receptors in the Hippocampus in a Mouse Model
    of Alzheimer’s Disease.” <i>Brain Pathology</i>, vol. 30, no. 3, Wiley, 2020,
    pp. 554–75, doi:<a href="https://doi.org/10.1111/bpa.12802">10.1111/bpa.12802</a>.
  short: A. Martín-Belmonte, C. Aguado, R. Alfaro-Ruíz, A.E. Moreno-Martínez, L. De
    La Ossa, J. Martínez-Hernández, A. Buisson, S. Früh, B. Bettler, R. Shigemoto,
    Y. Fukazawa, R. Luján, Brain Pathology 30 (2020) 554–575.
date_created: 2019-12-22T23:00:43Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-09-06T14:48:01Z
day: '01'
ddc:
- '570'
department:
- _id: RySh
doi: 10.1111/bpa.12802
ec_funded: 1
external_id:
  isi:
  - '000502270900001'
  pmid:
  - '31729777'
file:
- access_level: open_access
  checksum: 549cc1b18f638a21d17a939ba5563fa9
  content_type: application/pdf
  creator: dernst
  date_created: 2020-09-22T09:47:19Z
  date_updated: 2020-09-22T09:47:19Z
  file_id: '8554'
  file_name: 2020_BrainPathology_MartinBelmonte.pdf
  file_size: 4220935
  relation: main_file
  success: 1
file_date_updated: 2020-09-22T09:47:19Z
has_accepted_license: '1'
intvolume: '        30'
isi: 1
issue: '3'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 554-575
pmid: 1
project:
- _id: 25CBA828-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '720270'
  name: Human Brain Project Specific Grant Agreement 1 (HBP SGA 1)
- _id: 26436750-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '785907'
  name: Human Brain Project Specific Grant Agreement 2 (HBP SGA 2)
publication: Brain Pathology
publication_identifier:
  eissn:
  - '17503639'
  issn:
  - '10156305'
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Reduction in the neuronal surface of post and presynaptic GABA>B< receptors
  in the hippocampus in a mouse model of Alzheimer's disease
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2020'
...