---
_id: '11589'
abstract:
- lang: eng
  text: Calcium-dependent protein kinases (CPK) are key components of a wide array
    of signaling pathways, translating stress and nutrient signaling into the modulation
    of cellular processes such as ion transport and transcription. However, not much
    is known about CPKs in endomembrane trafficking. Here, we screened for CPKs that
    impact on root growth and gravitropism, by overexpressing constitutively active
    forms of CPKs under the control of an inducible promoter in Arabidopsis thaliana.
    We found that inducible overexpression of an constitutive active CPK30 (CA-CPK30)
    resulted in a loss of root gravitropism and ectopic auxin accumulation in the
    root tip. Immunolocalization revealed that CA-CPK30 roots have reduced PIN protein
    levels, PIN1 polarity defects and impaired Brefeldin A (BFA)-sensitive trafficking.
    Moreover, FM4-64 uptake was reduced, indicative of a defect in endocytosis. The
    effects on BFA-sensitive trafficking were not specific to PINs, as BFA could not
    induce aggregation of ARF1- and CHC-labeled endosomes in CA-CPK30. Interestingly,
    the interference with BFA-body formation, could be reverted by increasing the
    extracellular pH, indicating a pH-dependence of this CA-CPK30 effect. Altogether,
    our data reveal an important role for CPK30 in root growth regulation and endomembrane
    trafficking in Arabidopsis thaliana.
acknowledgement: "RW and JC predoctoral fellows that were supported by the Chinese
  Science Counsil. The IPS2 benefits from the support of the LabEx Saclay Plant Sciences-SPS
  (ANR-10-LABX-0040-SPS).\r\nWe thank Jen Sheen for establishing and generously sharing
  the CKP family clone sets, and for providing useful feedback on the manuscript."
article_number: '862398'
article_processing_charge: No
article_type: original
author:
- first_name: Ren
  full_name: Wang, Ren
  last_name: Wang
- first_name: Ellie
  full_name: Himschoot, Ellie
  last_name: Himschoot
- first_name: Jian
  full_name: Chen, Jian
  last_name: Chen
- first_name: Marie
  full_name: Boudsocq, Marie
  last_name: Boudsocq
- first_name: Danny
  full_name: Geelen, Danny
  last_name: Geelen
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Tom
  full_name: Beeckman, Tom
  last_name: Beeckman
- first_name: Steffen
  full_name: Vanneste, Steffen
  last_name: Vanneste
citation:
  ama: Wang R, Himschoot E, Chen J, et al. Constitutive active CPK30 interferes with
    root growth and endomembrane trafficking in Arabidopsis thaliana. <i>Frontiers
    in Plant Science</i>. 2022;13. doi:<a href="https://doi.org/10.3389/fpls.2022.862398">10.3389/fpls.2022.862398</a>
  apa: Wang, R., Himschoot, E., Chen, J., Boudsocq, M., Geelen, D., Friml, J., … Vanneste,
    S. (2022). Constitutive active CPK30 interferes with root growth and endomembrane
    trafficking in Arabidopsis thaliana. <i>Frontiers in Plant Science</i>. Frontiers.
    <a href="https://doi.org/10.3389/fpls.2022.862398">https://doi.org/10.3389/fpls.2022.862398</a>
  chicago: Wang, Ren, Ellie Himschoot, Jian Chen, Marie Boudsocq, Danny Geelen, Jiří
    Friml, Tom Beeckman, and Steffen Vanneste. “Constitutive Active CPK30 Interferes
    with Root Growth and Endomembrane Trafficking in Arabidopsis Thaliana.” <i>Frontiers
    in Plant Science</i>. Frontiers, 2022. <a href="https://doi.org/10.3389/fpls.2022.862398">https://doi.org/10.3389/fpls.2022.862398</a>.
  ieee: R. Wang <i>et al.</i>, “Constitutive active CPK30 interferes with root growth
    and endomembrane trafficking in Arabidopsis thaliana,” <i>Frontiers in Plant Science</i>,
    vol. 13. Frontiers, 2022.
  ista: Wang R, Himschoot E, Chen J, Boudsocq M, Geelen D, Friml J, Beeckman T, Vanneste
    S. 2022. Constitutive active CPK30 interferes with root growth and endomembrane
    trafficking in Arabidopsis thaliana. Frontiers in Plant Science. 13, 862398.
  mla: Wang, Ren, et al. “Constitutive Active CPK30 Interferes with Root Growth and
    Endomembrane Trafficking in Arabidopsis Thaliana.” <i>Frontiers in Plant Science</i>,
    vol. 13, 862398, Frontiers, 2022, doi:<a href="https://doi.org/10.3389/fpls.2022.862398">10.3389/fpls.2022.862398</a>.
  short: R. Wang, E. Himschoot, J. Chen, M. Boudsocq, D. Geelen, J. Friml, T. Beeckman,
    S. Vanneste, Frontiers in Plant Science 13 (2022).
date_created: 2022-07-17T22:01:54Z
date_published: 2022-06-16T00:00:00Z
date_updated: 2023-08-03T12:01:47Z
day: '16'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3389/fpls.2022.862398
external_id:
  isi:
  - '000819250500001'
  pmid:
  - '35783951'
file:
- access_level: open_access
  checksum: 95313515637c0f84de591d204375d764
  content_type: application/pdf
  creator: dernst
  date_created: 2022-07-18T08:05:15Z
  date_updated: 2022-07-18T08:05:15Z
  file_id: '11596'
  file_name: 2022_FrontiersPlantScience_Wang.pdf
  file_size: 5040638
  relation: main_file
  success: 1
file_date_updated: 2022-07-18T08:05:15Z
has_accepted_license: '1'
intvolume: '        13'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: Frontiers in Plant Science
publication_identifier:
  eissn:
  - 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
related_material:
  link:
  - relation: erratum
    url: https://doi.org/10.3389/fpls.2022.1100792
scopus_import: '1'
status: public
title: Constitutive active CPK30 interferes with root growth and endomembrane trafficking
  in Arabidopsis thaliana
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 13
year: '2022'
...
---
_id: '8924'
abstract:
- lang: eng
  text: 'Maintaining fertility in a fluctuating environment is key to the reproductive
    success of flowering plants. Meiosis and pollen formation are particularly sensitive
    to changes in growing conditions, especially temperature. We have previously identified
    cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent
    meiosis and this may involve the regulation of alternative splicing (AS), including
    of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing
    two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain
    which may be involved in co-factor interaction. In leaves, both isoforms have
    distinct temperature-dependent functions on target mRNA processing, but their
    role in pollen development is unknown. In the present study, we characterize the
    role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the
    long (L) form is necessary and sufficient to rescue the fertility defects of the
    cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the
    other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of
    the ATP binding pocket of the kinase indicates that kinase activity is necessary
    for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize
    to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to
    rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore,
    we show that there is partial functional overlap between CDKG1 and its paralog
    CDKG2 that could in part be explained by overlapping gene expression.'
acknowledgement: CN, DD, NF-F, and JD were funded by the BBSRC (grant number BB/M009459/1).
  NK and AM were funded through the ERASMUS+Program. NC was funded by the VIPS Program
  of the Austrian Federal Ministry of Science and Research and the City of Vienna.
article_number: '586870'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
  full_name: Nibau, Candida
  last_name: Nibau
- first_name: Despoina
  full_name: Dadarou, Despoina
  last_name: Dadarou
- first_name: Nestoras
  full_name: Kargios, Nestoras
  last_name: Kargios
- first_name: Areti
  full_name: Mallioura, Areti
  last_name: Mallioura
- first_name: Narcis
  full_name: Fernandez-Fuentes, Narcis
  last_name: Fernandez-Fuentes
- first_name: Nicola
  full_name: Cavallari, Nicola
  id: 457160E6-F248-11E8-B48F-1D18A9856A87
  last_name: Cavallari
- first_name: John H.
  full_name: Doonan, John H.
  last_name: Doonan
citation:
  ama: Nibau C, Dadarou D, Kargios N, et al. A functional kinase is necessary for
    cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
    in Arabidopsis. <i>Frontiers in Plant Science</i>. 2020;11. doi:<a href="https://doi.org/10.3389/fpls.2020.586870">10.3389/fpls.2020.586870</a>
  apa: Nibau, C., Dadarou, D., Kargios, N., Mallioura, A., Fernandez-Fuentes, N.,
    Cavallari, N., &#38; Doonan, J. H. (2020). A functional kinase is necessary for
    cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
    in Arabidopsis. <i>Frontiers in Plant Science</i>. Frontiers. <a href="https://doi.org/10.3389/fpls.2020.586870">https://doi.org/10.3389/fpls.2020.586870</a>
  chicago: Nibau, Candida, Despoina Dadarou, Nestoras Kargios, Areti Mallioura, Narcis
    Fernandez-Fuentes, Nicola Cavallari, and John H. Doonan. “A Functional Kinase
    Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High
    Ambient Temperature in Arabidopsis.” <i>Frontiers in Plant Science</i>. Frontiers,
    2020. <a href="https://doi.org/10.3389/fpls.2020.586870">https://doi.org/10.3389/fpls.2020.586870</a>.
  ieee: C. Nibau <i>et al.</i>, “A functional kinase is necessary for cyclin-dependent
    kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis,”
    <i>Frontiers in Plant Science</i>, vol. 11. Frontiers, 2020.
  ista: Nibau C, Dadarou D, Kargios N, Mallioura A, Fernandez-Fuentes N, Cavallari
    N, Doonan JH. 2020. A functional kinase is necessary for cyclin-dependent kinase
    G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. Frontiers
    in Plant Science. 11, 586870.
  mla: Nibau, Candida, et al. “A Functional Kinase Is Necessary for Cyclin-Dependent
    Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.”
    <i>Frontiers in Plant Science</i>, vol. 11, 586870, Frontiers, 2020, doi:<a href="https://doi.org/10.3389/fpls.2020.586870">10.3389/fpls.2020.586870</a>.
  short: C. Nibau, D. Dadarou, N. Kargios, A. Mallioura, N. Fernandez-Fuentes, N.
    Cavallari, J.H. Doonan, Frontiers in Plant Science 11 (2020).
date_created: 2020-12-06T23:01:14Z
date_published: 2020-11-10T00:00:00Z
date_updated: 2023-08-24T10:50:00Z
day: '10'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2020.586870
external_id:
  isi:
  - '000591637000001'
file:
- access_level: open_access
  checksum: 1c0ee6ce9950aa665d6a5cc64aa6b752
  content_type: application/pdf
  creator: dernst
  date_created: 2020-12-09T09:14:19Z
  date_updated: 2020-12-09T09:14:19Z
  file_id: '8929'
  file_name: 2020_Frontiers_Nibau.pdf
  file_size: 1833244
  relation: main_file
  success: 1
file_date_updated: 2020-12-09T09:14:19Z
has_accepted_license: '1'
intvolume: '        11'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_identifier:
  eissn:
  - 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to
  maintain fertility at high ambient temperature in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...