---
_id: '11588'
abstract:
- lang: eng
  text: Visualizing cell behavior and effector function on a single cell level has
    been crucial for understanding key aspects of mammalian biology. Due to their
    small size, large number and rapid recruitment into thrombi, there is a lack of
    data on fate and behavior of individual platelets in thrombosis and hemostasis.
    Here we report the use of platelet lineage restricted multi-color reporter mouse
    strains to delineate platelet function on a single cell level. We show that genetic
    labeling allows for single platelet and megakaryocyte (MK) tracking and morphological
    analysis in vivo and in vitro, while not affecting lineage functions. Using Cre-driven
    Confetti expression, we provide insights into temporal gene expression patterns
    as well as spatial clustering of MK in the bone marrow. In the vasculature, shape
    analysis of activated platelets recruited to thrombi identifies ubiquitous filopodia
    formation with no evidence of lamellipodia formation. Single cell tracking in
    complex thrombi reveals prominent myosin-dependent motility of platelets and highlights
    thrombus formation as a highly dynamic process amenable to modification and intervention
    of the acto-myosin cytoskeleton. Platelet function assays combining flow cytrometry,
    as well as in vivo, ex vivo and in vitro imaging show unaltered platelet functions
    of multicolor reporter mice compared to wild-type controls. In conclusion, platelet
    lineage multicolor reporter mice prove useful in furthering our understanding
    of platelet and MK biology on a single cell level.
acknowledgement: "This study was supported by the Deutsche Forschungsgemeinschaft
  (DFG) SFB 914 ( to SM [B02 and Z01]), the DFG SFB 1123 (to SM [B06]), the DFG FOR
  2033 (to SM), the German\r\nCenter for Cardiovascular Research (DZHK) (Clinician
  Scientist Programme), MHA 1.4VD (to SM), Postdoc Start-up Grant, 81X3600213 (to
  FG), 81X3600222 (to LN), the FP7 program\r\n(project 260309, PRESTIGE [to SM]).
  This project has received funding from the European Research Council (ERC) under
  the European Union’s Horizon 2020 research and innovation programme (grant agreement
  No. 83344, ERC-2018-ADG “IMMUNOTHROMBOSIS” [to SM] and the Marie Skłodowska Curie
  Individual Fellowship (EU project 747687, LamelliActin [to FG]). "
article_processing_charge: No
article_type: original
author:
- first_name: Leo
  full_name: Nicolai, Leo
  last_name: Nicolai
- first_name: Rainer
  full_name: Kaiser, Rainer
  last_name: Kaiser
- first_name: Raphael
  full_name: Escaig, Raphael
  last_name: Escaig
- first_name: Marie Louise
  full_name: Hoffknecht, Marie Louise
  last_name: Hoffknecht
- first_name: Afra
  full_name: Anjum, Afra
  last_name: Anjum
- first_name: Alexander
  full_name: Leunig, Alexander
  last_name: Leunig
- first_name: Joachim
  full_name: Pircher, Joachim
  last_name: Pircher
- first_name: Andreas
  full_name: Ehrlich, Andreas
  last_name: Ehrlich
- first_name: Michael
  full_name: Lorenz, Michael
  last_name: Lorenz
- first_name: Hellen
  full_name: Ishikawa-Ankerhold, Hellen
  last_name: Ishikawa-Ankerhold
- first_name: William C.
  full_name: Aird, William C.
  last_name: Aird
- first_name: Steffen
  full_name: Massberg, Steffen
  last_name: Massberg
- first_name: Florian R
  full_name: Gärtner, Florian R
  id: 397A88EE-F248-11E8-B48F-1D18A9856A87
  last_name: Gärtner
  orcid: 0000-0001-6120-3723
citation:
  ama: Nicolai L, Kaiser R, Escaig R, et al. Single platelet and megakaryocyte morpho-dynamics
    uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>.
    2022;107(7):1669-1680. doi:<a href="https://doi.org/10.3324/haematol.2021.278896">10.3324/haematol.2021.278896</a>
  apa: Nicolai, L., Kaiser, R., Escaig, R., Hoffknecht, M. L., Anjum, A., Leunig,
    A., … Gärtner, F. R. (2022). Single platelet and megakaryocyte morpho-dynamics
    uncovered by multicolor reporter mouse strains in vitro and in vivo. <i>Haematologica</i>.
    Ferrata Storti Foundation. <a href="https://doi.org/10.3324/haematol.2021.278896">https://doi.org/10.3324/haematol.2021.278896</a>
  chicago: Nicolai, Leo, Rainer Kaiser, Raphael Escaig, Marie Louise Hoffknecht, Afra
    Anjum, Alexander Leunig, Joachim Pircher, et al. “Single Platelet and Megakaryocyte
    Morpho-Dynamics Uncovered by Multicolor Reporter Mouse Strains in Vitro and in
    Vivo.” <i>Haematologica</i>. Ferrata Storti Foundation, 2022. <a href="https://doi.org/10.3324/haematol.2021.278896">https://doi.org/10.3324/haematol.2021.278896</a>.
  ieee: L. Nicolai <i>et al.</i>, “Single platelet and megakaryocyte morpho-dynamics
    uncovered by multicolor reporter mouse strains in vitro and in vivo,” <i>Haematologica</i>,
    vol. 107, no. 7. Ferrata Storti Foundation, pp. 1669–1680, 2022.
  ista: Nicolai L, Kaiser R, Escaig R, Hoffknecht ML, Anjum A, Leunig A, Pircher J,
    Ehrlich A, Lorenz M, Ishikawa-Ankerhold H, Aird WC, Massberg S, Gärtner FR. 2022.
    Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter
    mouse strains in vitro and in vivo. Haematologica. 107(7), 1669–1680.
  mla: Nicolai, Leo, et al. “Single Platelet and Megakaryocyte Morpho-Dynamics Uncovered
    by Multicolor Reporter Mouse Strains in Vitro and in Vivo.” <i>Haematologica</i>,
    vol. 107, no. 7, Ferrata Storti Foundation, 2022, pp. 1669–80, doi:<a href="https://doi.org/10.3324/haematol.2021.278896">10.3324/haematol.2021.278896</a>.
  short: L. Nicolai, R. Kaiser, R. Escaig, M.L. Hoffknecht, A. Anjum, A. Leunig, J.
    Pircher, A. Ehrlich, M. Lorenz, H. Ishikawa-Ankerhold, W.C. Aird, S. Massberg,
    F.R. Gärtner, Haematologica 107 (2022) 1669–1680.
date_created: 2022-07-17T22:01:54Z
date_published: 2022-07-01T00:00:00Z
date_updated: 2023-08-03T12:01:01Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.3324/haematol.2021.278896
ec_funded: 1
external_id:
  isi:
  - '000823746100018'
file:
- access_level: open_access
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  creator: dernst
  date_created: 2022-07-18T07:51:55Z
  date_updated: 2022-07-18T07:51:55Z
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file_date_updated: 2022-07-18T07:51:55Z
has_accepted_license: '1'
intvolume: '       107'
isi: 1
issue: '7'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 1669-1680
project:
- _id: 260AA4E2-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '747687'
  name: Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells
publication: Haematologica
publication_identifier:
  eissn:
  - 1592-8721
  issn:
  - 0390-6078
publication_status: published
publisher: Ferrata Storti Foundation
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single platelet and megakaryocyte morpho-dynamics uncovered by multicolor reporter
  mouse strains in vitro and in vivo
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 107
year: '2022'
...