---
_id: '3392'
abstract:
- lang: eng
  text: Migrating lymphocytes acquire a polarized phenotype with a leading and a trailing
    edge, or uropod. Although in vitro experiments in cell lines or activated primary
    cell cultures have established that Rho-p160 coiled-coil kinase (ROCK)-myosin
    II-mediated uropod contractility is required for integrin de-adhesion on two-dimensional
    surfaces and nuclear propulsion through narrow pores in three-dimensional matrices,
    less is known about the role of these two events during the recirculation of primary,
    nonactivated lymphocytes. Using pharmacological antagonists of ROCK and myosin
    II, we report that inhibition of uropod contractility blocked integrin-independent
    mouse T cell migration through narrow, but not large, pores in vitro. T cell crawling
    on chemokine-coated endothelial cells under shear was severely impaired by ROCK
    inhibition, whereas transendothelial migration was only reduced through endothelial
    cells with high, but not low, barrier properties. Using three-dimensional thick-tissue
    imaging and dynamic two-photon microscopy of T cell motility in lymphoid tissue,
    we demonstrated a significant role for uropod contractility in intraluminal crawling
    and transendothelial migration through lymph node, but not bone marrow, endothelial
    cells. Finally, we demonstrated that ICAM-1, but not anatomical constraints or
    integrin-independent interactions, reduced parenchymal motility of inhibitor-treated
    T cells within the dense lymphoid microenvironment, thus assigning context-dependent
    roles for uropod contraction during lymphocyte recirculation.
article_processing_charge: No
article_type: original
author:
- first_name: Silvia
  full_name: Soriano, Silvia
  last_name: Soriano
- first_name: Miroslav
  full_name: Hons, Miroslav
  last_name: Hons
  orcid: 0000-0002-6625-3348
- first_name: Kathrin
  full_name: Schumann, Kathrin
  last_name: Schumann
- first_name: Varsha
  full_name: Kumar, Varsha
  last_name: Kumar
- first_name: Timo
  full_name: Dennier, Timo
  last_name: Dennier
- first_name: Ruth
  full_name: Lyck, Ruth
  last_name: Lyck
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Jens
  full_name: Stein, Jens
  last_name: Stein
citation:
  ama: Soriano S, Hons M, Schumann K, et al. In vivo analysis of uropod function during
    physiological T cell trafficking. <i>Journal of Immunology</i>. 2011;187(5):2356-2364.
    doi:<a href="https://doi.org/10.4049/jimmunol.1100935">10.4049/jimmunol.1100935</a>
  apa: Soriano, S., Hons, M., Schumann, K., Kumar, V., Dennier, T., Lyck, R., … Stein,
    J. (2011). In vivo analysis of uropod function during physiological T cell trafficking.
    <i>Journal of Immunology</i>. American Association of Immunologists. <a href="https://doi.org/10.4049/jimmunol.1100935">https://doi.org/10.4049/jimmunol.1100935</a>
  chicago: Soriano, Silvia, Miroslav Hons, Kathrin Schumann, Varsha Kumar, Timo Dennier,
    Ruth Lyck, Michael K Sixt, and Jens Stein. “In Vivo Analysis of Uropod Function
    during Physiological T Cell Trafficking.” <i>Journal of Immunology</i>. American
    Association of Immunologists, 2011. <a href="https://doi.org/10.4049/jimmunol.1100935">https://doi.org/10.4049/jimmunol.1100935</a>.
  ieee: S. Soriano <i>et al.</i>, “In vivo analysis of uropod function during physiological
    T cell trafficking,” <i>Journal of Immunology</i>, vol. 187, no. 5. American Association
    of Immunologists, pp. 2356–2364, 2011.
  ista: Soriano S, Hons M, Schumann K, Kumar V, Dennier T, Lyck R, Sixt MK, Stein
    J. 2011. In vivo analysis of uropod function during physiological T cell trafficking.
    Journal of Immunology. 187(5), 2356–2364.
  mla: Soriano, Silvia, et al. “In Vivo Analysis of Uropod Function during Physiological
    T Cell Trafficking.” <i>Journal of Immunology</i>, vol. 187, no. 5, American Association
    of Immunologists, 2011, pp. 2356–64, doi:<a href="https://doi.org/10.4049/jimmunol.1100935">10.4049/jimmunol.1100935</a>.
  short: S. Soriano, M. Hons, K. Schumann, V. Kumar, T. Dennier, R. Lyck, M.K. Sixt,
    J. Stein, Journal of Immunology 187 (2011) 2356–2364.
date_created: 2018-12-11T12:03:04Z
date_published: 2011-09-01T00:00:00Z
date_updated: 2023-10-10T13:14:59Z
day: '01'
department:
- _id: MiSi
doi: 10.4049/jimmunol.1100935
intvolume: '       187'
issue: '5'
language:
- iso: eng
month: '09'
oa_version: None
page: 2356 - 2364
publication: Journal of Immunology
publication_identifier:
  eissn:
  - 1550-6606
  issn:
  - 0022-1767
publication_status: published
publisher: American Association of Immunologists
publist_id: '3215'
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vivo analysis of uropod function during physiological T cell trafficking
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 187
year: '2011'
...