---
_id: '9306'
abstract:
- lang: eng
  text: Assemblies of actin and its regulators underlie the dynamic morphology of
    all eukaryotic cells. To understand how actin regulatory proteins work together
    to generate actin-rich structures such as filopodia, we analyzed the localization
    of diverse actin regulators within filopodia in Drosophila embryos and in a complementary
    in vitro system of filopodia-like structures (FLSs). We found that the composition
    of the regulatory protein complex where actin is incorporated (the filopodial
    tip complex) is remarkably heterogeneous both in vivo and in vitro. Our data reveal
    that different pairs of proteins correlate with each other and with actin bundle
    length, suggesting the presence of functional subcomplexes. This is consistent
    with a theoretical framework where three or more redundant subcomplexes join the
    tip complex stochastically, with any two being sufficient to drive filopodia formation.
    We provide an explanation for the observed heterogeneity and suggest that a mechanism
    based on multiple components allows stereotypical filopodial dynamics to arise
    from diverse upstream signaling pathways.
acknowledgement: "This work was supported by European Research Council grant 281971,
  Wellcome Trust Research Career Development Fellowship WT095829AIA and Wellcome Trust
  Senior Research\r\nFellowship 219482/Z/19/Z to J.L. Gallop, a Wellcome Trust Senior
  Investigator Award 098357 to B.D. Simons, and an Austrian Science Fund grant (P31639)
  to E. Hannezo. We acknowledge\r\ncore funding by the Wellcome Trust (092096) and
  Cancer Research UK (C6946/A14492). U. Dobramysl was supported by a Wellcome Trust
  Junior Interdisciplinary Fellowship grant\r\n(105602/Z/14/Z) and a Herchel Smith
  Postdoctoral Fellowship. H. Shimo was supported by a Funai Foundation Overseas scholarship."
article_number: e202003052
article_processing_charge: No
article_type: original
author:
- first_name: Ulrich
  full_name: Dobramysl, Ulrich
  last_name: Dobramysl
- first_name: Iris Katharina
  full_name: Jarsch, Iris Katharina
  last_name: Jarsch
- first_name: Yoshiko
  full_name: Inoue, Yoshiko
  last_name: Inoue
- first_name: Hanae
  full_name: Shimo, Hanae
  last_name: Shimo
- first_name: Benjamin
  full_name: Richier, Benjamin
  last_name: Richier
- first_name: Jonathan R.
  full_name: Gadsby, Jonathan R.
  last_name: Gadsby
- first_name: Julia
  full_name: Mason, Julia
  last_name: Mason
- first_name: Alicja
  full_name: Szałapak, Alicja
  last_name: Szałapak
- first_name: Pantelis Savvas
  full_name: Ioannou, Pantelis Savvas
  last_name: Ioannou
- first_name: Guilherme Pereira
  full_name: Correia, Guilherme Pereira
  last_name: Correia
- first_name: Astrid
  full_name: Walrant, Astrid
  last_name: Walrant
- first_name: Richard
  full_name: Butler, Richard
  last_name: Butler
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Benjamin D.
  full_name: Simons, Benjamin D.
  last_name: Simons
- first_name: Jennifer L.
  full_name: Gallop, Jennifer L.
  last_name: Gallop
citation:
  ama: Dobramysl U, Jarsch IK, Inoue Y, et al. Stochastic combinations of actin regulatory
    proteins are sufficient to drive filopodia formation. <i>Journal of Cell Biology</i>.
    2021;220(4). doi:<a href="https://doi.org/10.1083/jcb.202003052">10.1083/jcb.202003052</a>
  apa: Dobramysl, U., Jarsch, I. K., Inoue, Y., Shimo, H., Richier, B., Gadsby, J.
    R., … Gallop, J. L. (2021). Stochastic combinations of actin regulatory proteins
    are sufficient to drive filopodia formation. <i>Journal of Cell Biology</i>. Rockefeller
    University Press. <a href="https://doi.org/10.1083/jcb.202003052">https://doi.org/10.1083/jcb.202003052</a>
  chicago: Dobramysl, Ulrich, Iris Katharina Jarsch, Yoshiko Inoue, Hanae Shimo, Benjamin
    Richier, Jonathan R. Gadsby, Julia Mason, et al. “Stochastic Combinations of Actin
    Regulatory Proteins Are Sufficient to Drive Filopodia Formation.” <i>Journal of
    Cell Biology</i>. Rockefeller University Press, 2021. <a href="https://doi.org/10.1083/jcb.202003052">https://doi.org/10.1083/jcb.202003052</a>.
  ieee: U. Dobramysl <i>et al.</i>, “Stochastic combinations of actin regulatory proteins
    are sufficient to drive filopodia formation,” <i>Journal of Cell Biology</i>,
    vol. 220, no. 4. Rockefeller University Press, 2021.
  ista: Dobramysl U, Jarsch IK, Inoue Y, Shimo H, Richier B, Gadsby JR, Mason J, Szałapak
    A, Ioannou PS, Correia GP, Walrant A, Butler R, Hannezo EB, Simons BD, Gallop
    JL. 2021. Stochastic combinations of actin regulatory proteins are sufficient
    to drive filopodia formation. Journal of Cell Biology. 220(4), e202003052.
  mla: Dobramysl, Ulrich, et al. “Stochastic Combinations of Actin Regulatory Proteins
    Are Sufficient to Drive Filopodia Formation.” <i>Journal of Cell Biology</i>,
    vol. 220, no. 4, e202003052, Rockefeller University Press, 2021, doi:<a href="https://doi.org/10.1083/jcb.202003052">10.1083/jcb.202003052</a>.
  short: U. Dobramysl, I.K. Jarsch, Y. Inoue, H. Shimo, B. Richier, J.R. Gadsby, J.
    Mason, A. Szałapak, P.S. Ioannou, G.P. Correia, A. Walrant, R. Butler, E.B. Hannezo,
    B.D. Simons, J.L. Gallop, Journal of Cell Biology 220 (2021).
date_created: 2021-04-04T22:01:21Z
date_published: 2021-03-19T00:00:00Z
date_updated: 2023-08-07T14:32:28Z
day: '19'
ddc:
- '576'
department:
- _id: EdHa
doi: 10.1083/jcb.202003052
external_id:
  isi:
  - '000663160600002'
  pmid:
  - '33740033'
file:
- access_level: open_access
  checksum: 4739ffd90f2c7e05ac5b00f057c58aa2
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  creator: dernst
  date_created: 2021-04-06T10:39:08Z
  date_updated: 2021-04-06T10:39:08Z
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has_accepted_license: '1'
intvolume: '       220'
isi: 1
issue: '4'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 268294B6-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P31639
  name: Active mechano-chemical description of the cell cytoskeleton
publication: Journal of Cell Biology
publication_identifier:
  eissn:
  - '15408140'
publication_status: published
publisher: Rockefeller University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Stochastic combinations of actin regulatory proteins are sufficient to drive
  filopodia formation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 220
year: '2021'
...