---
_id: '7949'
abstract:
- lang: eng
text: Peptides derived from non-functional precursors play important roles in various
developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide
analyses of C-terminally encoded peptide 5 (CEP5)-mediated changes revealed an
impact on abiotic stress-related processes. Drought has a dramatic impact on plant
growth, development and reproduction, and the plant hormone auxin plays a role
in drought responses. Our genetic, physiological, biochemical and pharmacological
results demonstrated that CEP5-mediated signaling is relevant for osmotic and
drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts
auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional
repressors, suggesting the existence of a novel peptide-dependent control mechanism
that tunes auxin signaling. These observations align with the recently described
role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic
and drought stress tolerance.
acknowledgement: We thank Maria Njo, Sarah De Cokere, Marieke Mispelaere and Darren
Wells, for practical assistance, Daniël Van Damme for assistance with image analysis,
Marnik Vuylsteke for advice on statistics, Catherine Perrot-Rechenmann for useful
discussions, Steffen Lau for critical reading oft he manuscript, and Philip Benfey,
Gerd Jürgens, Philippe Nacry, Frederik Börnke, and Frans Tax for sharing materials.
article_processing_charge: No
article_type: original
author:
- first_name: S
full_name: Smith, S
last_name: Smith
- first_name: S
full_name: Zhu, S
last_name: Zhu
- first_name: L
full_name: Joos, L
last_name: Joos
- first_name: I
full_name: Roberts, I
last_name: Roberts
- first_name: N
full_name: Nikonorova, N
last_name: Nikonorova
- first_name: LD
full_name: Vu, LD
last_name: Vu
- first_name: E
full_name: Stes, E
last_name: Stes
- first_name: H
full_name: Cho, H
last_name: Cho
- first_name: A
full_name: Larrieu, A
last_name: Larrieu
- first_name: W
full_name: Xuan, W
last_name: Xuan
- first_name: B
full_name: Goodall, B
last_name: Goodall
- first_name: B
full_name: van de Cotte, B
last_name: van de Cotte
- first_name: JM
full_name: Waite, JM
last_name: Waite
- first_name: A
full_name: Rigal, A
last_name: Rigal
- first_name: SR
full_name: R Harborough, SR
last_name: R Harborough
- first_name: G
full_name: Persiau, G
last_name: Persiau
- first_name: S
full_name: Vanneste, S
last_name: Vanneste
- first_name: GK
full_name: Kirschner, GK
last_name: Kirschner
- first_name: E
full_name: Vandermarliere, E
last_name: Vandermarliere
- first_name: L
full_name: Martens, L
last_name: Martens
- first_name: Y
full_name: Stahl, Y
last_name: Stahl
- first_name: D
full_name: Audenaert, D
last_name: Audenaert
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: G
full_name: Felix, G
last_name: Felix
- first_name: R
full_name: Simon, R
last_name: Simon
- first_name: M
full_name: Bennett, M
last_name: Bennett
- first_name: A
full_name: Bishopp, A
last_name: Bishopp
- first_name: G
full_name: De Jaeger, G
last_name: De Jaeger
- first_name: K
full_name: Ljung, K
last_name: Ljung
- first_name: S
full_name: Kepinski, S
last_name: Kepinski
- first_name: S
full_name: Robert, S
last_name: Robert
- first_name: J
full_name: Nemhauser, J
last_name: Nemhauser
- first_name: I
full_name: Hwang, I
last_name: Hwang
- first_name: K
full_name: Gevaert, K
last_name: Gevaert
- first_name: T
full_name: Beeckman, T
last_name: Beeckman
- first_name: I
full_name: De Smet, I
last_name: De Smet
citation:
ama: Smith S, Zhu S, Joos L, et al. The CEP5 peptide promotes abiotic stress tolerance,
as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium
in Arabidopsis. Molecular & Cellular Proteomics. 2020;19(8):1248-1262.
doi:10.1074/mcp.ra119.001826
apa: Smith, S., Zhu, S., Joos, L., Roberts, I., Nikonorova, N., Vu, L., … De Smet,
I. (2020). The CEP5 peptide promotes abiotic stress tolerance, as revealed by
quantitative proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis.
Molecular & Cellular Proteomics. American Society for Biochemistry
and Molecular Biology. https://doi.org/10.1074/mcp.ra119.001826
chicago: Smith, S, S Zhu, L Joos, I Roberts, N Nikonorova, LD Vu, E Stes, et al.
“The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed by Quantitative
Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.” Molecular
& Cellular Proteomics. American Society for Biochemistry and Molecular
Biology, 2020. https://doi.org/10.1074/mcp.ra119.001826.
ieee: S. Smith et al., “The CEP5 peptide promotes abiotic stress tolerance,
as revealed by quantitative proteomics, and attenuates the AUX/IAA equilibrium
in Arabidopsis,” Molecular & Cellular Proteomics, vol. 19, no. 8. American
Society for Biochemistry and Molecular Biology, pp. 1248–1262, 2020.
ista: Smith S, Zhu S, Joos L, Roberts I, Nikonorova N, Vu L, Stes E, Cho H, Larrieu
A, Xuan W, Goodall B, van de Cotte B, Waite J, Rigal A, R Harborough S, Persiau
G, Vanneste S, Kirschner G, Vandermarliere E, Martens L, Stahl Y, Audenaert D,
Friml J, Felix G, Simon R, Bennett M, Bishopp A, De Jaeger G, Ljung K, Kepinski
S, Robert S, Nemhauser J, Hwang I, Gevaert K, Beeckman T, De Smet I. 2020. The
CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative proteomics,
and attenuates the AUX/IAA equilibrium in Arabidopsis. Molecular & Cellular
Proteomics. 19(8), 1248–1262.
mla: Smith, S., et al. “The CEP5 Peptide Promotes Abiotic Stress Tolerance, as Revealed
by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis.”
Molecular & Cellular Proteomics, vol. 19, no. 8, American Society for
Biochemistry and Molecular Biology, 2020, pp. 1248–62, doi:10.1074/mcp.ra119.001826.
short: S. Smith, S. Zhu, L. Joos, I. Roberts, N. Nikonorova, L. Vu, E. Stes, H.
Cho, A. Larrieu, W. Xuan, B. Goodall, B. van de Cotte, J. Waite, A. Rigal, S.
R Harborough, G. Persiau, S. Vanneste, G. Kirschner, E. Vandermarliere, L. Martens,
Y. Stahl, D. Audenaert, J. Friml, G. Felix, R. Simon, M. Bennett, A. Bishopp,
G. De Jaeger, K. Ljung, S. Kepinski, S. Robert, J. Nemhauser, I. Hwang, K. Gevaert,
T. Beeckman, I. De Smet, Molecular & Cellular Proteomics 19 (2020) 1248–1262.
date_created: 2020-06-08T10:10:53Z
date_published: 2020-08-01T00:00:00Z
date_updated: 2023-09-05T12:17:46Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1074/mcp.ra119.001826
external_id:
isi:
- '000561114000001'
pmid:
- '32404488'
file:
- access_level: open_access
checksum: 3f3f37b4a1ba2cfd270fc7733dd89680
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creator: kschuh
date_created: 2021-05-05T10:10:14Z
date_updated: 2021-05-05T10:10:14Z
file_id: '9373'
file_name: 2020_MCP_Smith.pdf
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relation: main_file
success: 1
file_date_updated: 2021-05-05T10:10:14Z
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intvolume: ' 19'
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issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
page: 1248-1262
pmid: 1
publication: Molecular & Cellular Proteomics
publication_identifier:
eissn:
- 1535-9484
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
quality_controlled: '1'
scopus_import: '1'
status: public
title: The CEP5 peptide promotes abiotic stress tolerance, as revealed by quantitative
proteomics, and attenuates the AUX/IAA equilibrium in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2020'
...