[{"author":[{"full_name":"Dahhan, DA","last_name":"Dahhan","first_name":"DA"},{"full_name":"Reynolds, GD","first_name":"GD","last_name":"Reynolds"},{"full_name":"Cárdenas, JJ","first_name":"JJ","last_name":"Cárdenas"},{"last_name":"Eeckhout","first_name":"D","full_name":"Eeckhout, D"},{"id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","first_name":"Alexander J","last_name":"Johnson"},{"first_name":"K","last_name":"Yperman","full_name":"Yperman, K"},{"last_name":"Kaufmann","first_name":"Walter","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9735-5315"},{"last_name":"Vang","first_name":"N","full_name":"Vang, N"},{"full_name":"Yan, X","last_name":"Yan","first_name":"X"},{"full_name":"Hwang, I","first_name":"I","last_name":"Hwang"},{"full_name":"Heese, A","first_name":"A","last_name":"Heese"},{"full_name":"De Jaeger, G","last_name":"De Jaeger","first_name":"G"},{"first_name":"Jiří","last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"},{"full_name":"Van Damme, D","last_name":"Van Damme","first_name":"D"},{"last_name":"Pan","first_name":"J","full_name":"Pan, J"},{"full_name":"Bednarek, SY","first_name":"SY","last_name":"Bednarek"}],"day":"01","title":"Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components","publisher":"Oxford Academic","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"JiFr"},{"_id":"EM-Fac"}],"pmid":1,"publication":"Plant Cell","article_type":"original","scopus_import":"1","article_processing_charge":"No","publication_identifier":{"eissn":["1532-298x"],"issn":["1040-4651"]},"doi":"10.1093/plcell/koac071","quality_controlled":"1","project":[{"grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"isi":1,"issue":"6","language":[{"iso":"eng"}],"page":"2150-2173","month":"06","type":"journal_article","oa_version":"Preprint","date_updated":"2023-08-02T14:46:48Z","abstract":[{"text":"In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor-protein assembly polypeptide-1 (AP-1) complex operates as part of the secretory pathway at the trans-Golgi network (TGN), while the AP-2 complex and the TPLATE complex jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant-specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched TGN/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis (Arabidopsis thaliana) cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data.","lang":"eng"}],"volume":34,"date_created":"2022-03-08T13:47:51Z","acknowledgement":"The authors would like to acknowledge the VIB Proteomics Core Facility (VIB-UGent Center for Medical Biotechnology in Ghent, Belgium) and the Research Technology Support Facility Proteomics Core (Michigan State University in East Lansing, Michigan) for sample analysis, as well as the University of Wisconsin Biotechnology Center Mass Spectrometry Core Facility (Madison, WI) for help with data processing. Additionally, we are grateful to Sue Weintraub (UT Health San Antonio) and Sydney Thomas (UW- Madison) for assistance with data analysis. This research was supported by grants to S.Y.B. from the National Science Foundation (Nos. 1121998 and 1614915) and a Vilas Associate Award (University of Wisconsin, Madison, Graduate School); to J.P. from the National Natural Science Foundation of China (Nos. 91754104, 31820103008, and 31670283); to I.H. from the National Research Foundation of Korea (No. 2019R1A2B5B03099982). This research was also supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Electron microscopy Facility (EMF). A.J. is supported by funding from the Austrian Science Fund (FWF): I3630B25 to J.F. A.H. is supported by funding from the National Science Foundation (NSF IOS Nos. 1025837 and 1147032).","year":"2022","_id":"10841","oa":1,"publication_status":"published","date_published":"2022-06-01T00:00:00Z","acknowledged_ssus":[{"_id":"EM-Fac"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2021.09.16.460678"}],"status":"public","external_id":{"pmid":["35218346"],"isi":["000767438800001"]},"intvolume":"        34","citation":{"chicago":"Dahhan, DA, GD Reynolds, JJ Cárdenas, D Eeckhout, Alexander J Johnson, K Yperman, Walter Kaufmann, et al. “Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.” <i>Plant Cell</i>. Oxford Academic, 2022. <a href=\"https://doi.org/10.1093/plcell/koac071\">https://doi.org/10.1093/plcell/koac071</a>.","ieee":"D. Dahhan <i>et al.</i>, “Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components,” <i>Plant Cell</i>, vol. 34, no. 6. Oxford Academic, pp. 2150–2173, 2022.","short":"D. Dahhan, G. Reynolds, J. Cárdenas, D. Eeckhout, A.J. Johnson, K. Yperman, W. Kaufmann, N. Vang, X. Yan, I. Hwang, A. Heese, G. De Jaeger, J. Friml, D. Van Damme, J. Pan, S. Bednarek, Plant Cell 34 (2022) 2150–2173.","ama":"Dahhan D, Reynolds G, Cárdenas J, et al. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. <i>Plant Cell</i>. 2022;34(6):2150-2173. doi:<a href=\"https://doi.org/10.1093/plcell/koac071\">10.1093/plcell/koac071</a>","apa":"Dahhan, D., Reynolds, G., Cárdenas, J., Eeckhout, D., Johnson, A. J., Yperman, K., … Bednarek, S. (2022). Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. <i>Plant Cell</i>. Oxford Academic. <a href=\"https://doi.org/10.1093/plcell/koac071\">https://doi.org/10.1093/plcell/koac071</a>","mla":"Dahhan, DA, et al. “Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.” <i>Plant Cell</i>, vol. 34, no. 6, Oxford Academic, 2022, pp. 2150–73, doi:<a href=\"https://doi.org/10.1093/plcell/koac071\">10.1093/plcell/koac071</a>.","ista":"Dahhan D, Reynolds G, Cárdenas J, Eeckhout D, Johnson AJ, Yperman K, Kaufmann W, Vang N, Yan X, Hwang I, Heese A, De Jaeger G, Friml J, Van Damme D, Pan J, Bednarek S. 2022. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. Plant Cell. 34(6), 2150–2173."}},{"date_created":"2021-06-02T13:13:58Z","file_date_updated":"2021-10-14T13:36:38Z","volume":33,"type":"journal_article","month":"07","oa_version":"Published Version","date_updated":"2023-08-08T13:54:32Z","abstract":[{"lang":"eng","text":"Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER–PM tether that also functions in maintaining PM integrity. The ER–PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild-type while the levels of most glycerolipid species remain unchanged. In addition, the SYT1-green fluorescent protein fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress."}],"page":"2431-2453","_id":"9443","year":"2021","acknowledgement":"We would also like to thank Lothar Willmitzer for the lipidomic analysis at the Max Planck Institute of Molecular Plant Physiology (Potsdam, Germany). We thank Manuela Vega from SCI for her technical assistance in image analysis. We thank John R. Pearson and the Bionand Nanoimaging Unit, F. David Navas Fernández and the SCAI Imaging Facility and The Plant Cell Biology facility at the Shanghai Center for Plant Stress Biology for assistance with confocal microscopy. The FaFAH1 clone was a gift from Iraida Amaya Saavedra (IFAPA-Centro de Churriana, Málaga, Spain). The AHA3 antibody against the H+-ATPase was a gift from Ramón Serrano Salom (Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain). The MAP-mTU2-SAC1 construct was provided by Yvon Jaillais (Laboratoire Reproduction et Développement des Plantes, Univ Lyon, France). The pGWB5 from the pGWB vector series, was provided by Tsuyoshi Nakagawa (Department of Molecular and Functional Genomics, Shimane University). We thank Plan Propio from the University of Málaga for financial support.\r\nFunding","ddc":["580"],"date_published":"2021-07-01T00:00:00Z","has_accepted_license":"1","publication_status":"published","oa":1,"citation":{"short":"N. Ruiz-Lopez, J. Pérez-Sancho, A. Esteban Del Valle, R. Haslam, S. Vanneste, R. Catalá, C. Perea-Resa, D. Van Damme, S. García-Hernández, A. Albert, J. Vallarino, J. Lin, J. Friml, A. Macho, J. Salinas, A. Rosado, J. Napier, V. Amorim-Silva, M. Botella, Plant Cell 33 (2021) 2431–2453.","chicago":"Ruiz-Lopez, N, J Pérez-Sancho, A Esteban Del Valle, RP Haslam, S Vanneste, R Catalá, C Perea-Resa, et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.” <i>Plant Cell</i>. American Society of Plant Biologists, 2021. <a href=\"https://doi.org/10.1093/plcell/koab122\">https://doi.org/10.1093/plcell/koab122</a>.","ieee":"N. Ruiz-Lopez <i>et al.</i>, “Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress,” <i>Plant Cell</i>, vol. 33, no. 7. American Society of Plant Biologists, pp. 2431–2453, 2021.","apa":"Ruiz-Lopez, N., Pérez-Sancho, J., Esteban Del Valle, A., Haslam, R., Vanneste, S., Catalá, R., … Botella, M. (2021). Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plcell/koab122\">https://doi.org/10.1093/plcell/koab122</a>","ista":"Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, Haslam R, Vanneste S, Catalá R, Perea-Resa C, Van Damme D, García-Hernández S, Albert A, Vallarino J, Lin J, Friml J, Macho A, Salinas J, Rosado A, Napier J, Amorim-Silva V, Botella M. 2021. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. Plant Cell. 33(7), 2431–2453.","mla":"Ruiz-Lopez, N., et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.” <i>Plant Cell</i>, vol. 33, no. 7, American Society of Plant Biologists, 2021, pp. 2431–53, doi:<a href=\"https://doi.org/10.1093/plcell/koab122\">10.1093/plcell/koab122</a>.","ama":"Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, et al. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. <i>Plant Cell</i>. 2021;33(7):2431-2453. doi:<a href=\"https://doi.org/10.1093/plcell/koab122\">10.1093/plcell/koab122</a>"},"intvolume":"        33","status":"public","external_id":{"isi":["000703938100026"],"pmid":["33944955"]},"title":"Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress","file":[{"access_level":"open_access","date_created":"2021-10-14T13:36:38Z","checksum":"22d596678d00310d793611864a6d0fcd","file_id":"10141","date_updated":"2021-10-14T13:36:38Z","creator":"cchlebak","file_size":2952028,"relation":"main_file","content_type":"application/pdf","file_name":"2021_PlantCell_RuizLopez.pdf","success":1}],"day":"01","author":[{"full_name":"Ruiz-Lopez, N","first_name":"N","last_name":"Ruiz-Lopez"},{"last_name":"Pérez-Sancho","first_name":"J","full_name":"Pérez-Sancho, J"},{"last_name":"Esteban Del Valle","first_name":"A","full_name":"Esteban Del Valle, A"},{"full_name":"Haslam, RP","first_name":"RP","last_name":"Haslam"},{"full_name":"Vanneste, S","first_name":"S","last_name":"Vanneste"},{"first_name":"R","last_name":"Catalá","full_name":"Catalá, R"},{"last_name":"Perea-Resa","first_name":"C","full_name":"Perea-Resa, C"},{"full_name":"Van Damme, D","last_name":"Van Damme","first_name":"D"},{"full_name":"García-Hernández, S","first_name":"S","last_name":"García-Hernández"},{"full_name":"Albert, A","first_name":"A","last_name":"Albert"},{"first_name":"J","last_name":"Vallarino","full_name":"Vallarino, J"},{"first_name":"J","last_name":"Lin","full_name":"Lin, J"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"first_name":"AP","last_name":"Macho","full_name":"Macho, AP"},{"last_name":"Salinas","first_name":"J","full_name":"Salinas, J"},{"last_name":"Rosado","first_name":"A","full_name":"Rosado, A"},{"full_name":"Napier, JA","first_name":"JA","last_name":"Napier"},{"full_name":"Amorim-Silva, V","last_name":"Amorim-Silva","first_name":"V"},{"first_name":"MA","last_name":"Botella","full_name":"Botella, MA"}],"tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"article_type":"original","ec_funded":1,"scopus_import":"1","article_processing_charge":"No","publication":"Plant Cell","department":[{"_id":"JiFr"}],"pmid":1,"publisher":"American Society of Plant Biologists","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","quality_controlled":"1","doi":"10.1093/plcell/koab122","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"issue":"7","language":[{"iso":"eng"}],"isi":1,"project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985"}]},{"date_updated":"2023-08-10T14:01:41Z","abstract":[{"lang":"eng","text":"To overcome nitrogen deficiency, legume roots establish symbiotic interactions with nitrogen-fixing rhizobia that is fostered in specialized organs (nodules). Similar to other organs, nodule formation is determined by a local maximum of the phytohormone auxin at the primordium site. However, how auxin regulates nodule development remains poorly understood. Here, we found that in soybean, (Glycine max), dynamic auxin transport driven by PIN-FORMED (PIN) transporter GmPIN1 is involved in nodule primordium formation. GmPIN1 was specifically expressed in nodule primordium cells and GmPIN1 was polarly localized in these cells. Two nodulation regulators, (iso)flavonoids trigger expanded distribution of GmPIN1b to root cortical cells, and cytokinin rearranges GmPIN1b polarity. Gmpin1abc triple mutants generated with CRISPR-Cas9 showed impaired establishment of auxin maxima in nodule meristems and aberrant divisions in the nodule primordium cells. Moreover, overexpression of GmPIN1 suppressed nodule primordium initiation. GmPIN9d, an ortholog of Arabidopsis thaliana PIN2, acts together with GmPIN1 later in nodule development to acropetally transport auxin in vascular bundles, fine-tuning the auxin supply for nodule enlargement. Our findings reveal how PIN-dependent auxin transport modulates different aspects of soybean nodule development and suggest that establishment of auxin gradient is a prerequisite for the proper interaction between legumes and rhizobia."}],"month":"07","oa_version":"Published Version","type":"journal_article","page":"2981–3003","file_date_updated":"2021-07-19T12:13:34Z","date_created":"2021-07-14T15:32:43Z","volume":33,"year":"2021","_id":"9657","has_accepted_license":"1","publication_status":"published","oa":1,"ddc":["580"],"date_published":"2021-07-07T00:00:00Z","status":"public","external_id":{"isi":["000702165300012"],"pmid":["34240197"]},"citation":{"ista":"Gao Z, Chen Z, Cui Y, Ke M, Xu H, Xu Q, Chen J, Li Y, Huang L, Zhao H, Huang D, Mai S, Xu T, Liu X, Li S, Guan Y, Yang W, Friml J, Petrášek J, Zhang J, Chen X. 2021. GmPIN-dependent polar auxin transport is involved in soybean nodule development. Plant Cell. 33(9), 2981–3003.","mla":"Gao, Z., et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean Nodule Development.” <i>Plant Cell</i>, vol. 33, no. 9, American Society of Plant Biologists, 2021, pp. 2981–3003, doi:<a href=\"https://doi.org/10.1093/plcell/koab183\">10.1093/plcell/koab183</a>.","apa":"Gao, Z., Chen, Z., Cui, Y., Ke, M., Xu, H., Xu, Q., … Chen, X. (2021). GmPIN-dependent polar auxin transport is involved in soybean nodule development. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plcell/koab183\">https://doi.org/10.1093/plcell/koab183</a>","ama":"Gao Z, Chen Z, Cui Y, et al. GmPIN-dependent polar auxin transport is involved in soybean nodule development. <i>Plant Cell</i>. 2021;33(9):2981–3003. doi:<a href=\"https://doi.org/10.1093/plcell/koab183\">10.1093/plcell/koab183</a>","short":"Z. Gao, Z. Chen, Y. Cui, M. Ke, H. Xu, Q. Xu, J. Chen, Y. Li, L. Huang, H. Zhao, D. Huang, S. Mai, T. Xu, X. Liu, S. Li, Y. Guan, W. Yang, J. Friml, J. Petrášek, J. Zhang, X. Chen, Plant Cell 33 (2021) 2981–3003.","ieee":"Z. Gao <i>et al.</i>, “GmPIN-dependent polar auxin transport is involved in soybean nodule development,” <i>Plant Cell</i>, vol. 33, no. 9. American Society of Plant Biologists, pp. 2981–3003, 2021.","chicago":"Gao, Z, Z Chen, Y Cui, M Ke, H Xu, Q Xu, J Chen, et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean Nodule Development.” <i>Plant Cell</i>. American Society of Plant Biologists, 2021. <a href=\"https://doi.org/10.1093/plcell/koab183\">https://doi.org/10.1093/plcell/koab183</a>."},"intvolume":"        33","day":"07","file":[{"access_level":"open_access","date_created":"2021-07-19T12:13:34Z","checksum":"6715712ec306c321f0204c817b7f8ae7","date_updated":"2021-07-19T12:13:34Z","file_id":"9691","creator":"cziletti","content_type":"application/pdf","relation":"main_file","file_size":10566921,"success":1,"file_name":"2021_PlantCell_Gao.pdf"}],"author":[{"full_name":"Gao, Z","first_name":"Z","last_name":"Gao"},{"full_name":"Chen, Z","last_name":"Chen","first_name":"Z"},{"last_name":"Cui","first_name":"Y","full_name":"Cui, Y"},{"first_name":"M","last_name":"Ke","full_name":"Ke, M"},{"last_name":"Xu","first_name":"H","full_name":"Xu, H"},{"last_name":"Xu","first_name":"Q","full_name":"Xu, Q"},{"full_name":"Chen, J","first_name":"J","last_name":"Chen"},{"full_name":"Li, Y","first_name":"Y","last_name":"Li"},{"full_name":"Huang, L","last_name":"Huang","first_name":"L"},{"full_name":"Zhao, H","first_name":"H","last_name":"Zhao"},{"full_name":"Huang, D","first_name":"D","last_name":"Huang"},{"first_name":"S","last_name":"Mai","full_name":"Mai, S"},{"first_name":"T","last_name":"Xu","full_name":"Xu, T"},{"full_name":"Liu, X","last_name":"Liu","first_name":"X"},{"full_name":"Li, S","first_name":"S","last_name":"Li"},{"first_name":"Y","last_name":"Guan","full_name":"Guan, Y"},{"first_name":"W","last_name":"Yang","full_name":"Yang, W"},{"last_name":"Friml","first_name":"Jiří","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596"},{"last_name":"Petrášek","first_name":"J","full_name":"Petrášek, J"},{"full_name":"Zhang, J","last_name":"Zhang","first_name":"J"},{"first_name":"X","last_name":"Chen","full_name":"Chen, X"}],"title":"GmPIN-dependent polar auxin transport is involved in soybean nodule development","pmid":1,"department":[{"_id":"JiFr"}],"publisher":"American Society of Plant Biologists","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_processing_charge":"No","article_type":"original","tmp":{"name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode"},"publication":"Plant Cell","publication_identifier":{"eissn":["1532-298x"],"issn":["1040-4651"]},"quality_controlled":"1","doi":"10.1093/plcell/koab183","language":[{"iso":"eng"}],"issue":"9","isi":1},{"project":[{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"grant_number":"742985","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"}],"issue":"11","language":[{"iso":"eng"}],"isi":1,"publication_identifier":{"eissn":["1532-298x"],"issn":["1040-4651"]},"quality_controlled":"1","doi":"10.1105/tpc.20.00384","department":[{"_id":"JiFr"}],"pmid":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"American Society of Plant Biologists","article_type":"original","article_processing_charge":"No","ec_funded":1,"scopus_import":"1","publication":"Plant Cell","day":"01","author":[{"first_name":"D","last_name":"Liu","full_name":"Liu, D"},{"first_name":"R","last_name":"Kumar","full_name":"Kumar, R"},{"full_name":"LAN, Claus","first_name":"Claus","last_name":"LAN"},{"id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","last_name":"Johnson","first_name":"Alexander J"},{"first_name":"W","last_name":"Siao","full_name":"Siao, W"},{"last_name":"Vanhoutte","first_name":"I","full_name":"Vanhoutte, I"},{"full_name":"Wang, P","last_name":"Wang","first_name":"P"},{"first_name":"KW","last_name":"Bender","full_name":"Bender, KW"},{"last_name":"Yperman","first_name":"K","full_name":"Yperman, K"},{"full_name":"Martins, S","first_name":"S","last_name":"Martins"},{"full_name":"Zhao, X","last_name":"Zhao","first_name":"X"},{"last_name":"Vert","first_name":"G","full_name":"Vert, G"},{"full_name":"Van Damme, D","first_name":"D","last_name":"Van Damme"},{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"full_name":"Russinova, E","last_name":"Russinova","first_name":"E"}],"title":"Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif","external_id":{"isi":["000600226800021"],"pmid":["32958564"]},"status":"public","citation":{"chicago":"Liu, D, R Kumar, Claus LAN, Alexander J Johnson, W Siao, I Vanhoutte, P Wang, et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>.","ieee":"D. Liu <i>et al.</i>, “Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif,” <i>Plant Cell</i>, vol. 32, no. 11. American Society of Plant Biologists, pp. 3598–3612, 2020.","short":"D. Liu, R. Kumar, C. LAN, A.J. Johnson, W. Siao, I. Vanhoutte, P. Wang, K. Bender, K. Yperman, S. Martins, X. Zhao, G. Vert, D. Van Damme, J. Friml, E. Russinova, Plant Cell 32 (2020) 3598–3612.","ama":"Liu D, Kumar R, LAN C, et al. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. 2020;32(11):3598-3612. doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>","apa":"Liu, D., Kumar, R., LAN, C., Johnson, A. J., Siao, W., Vanhoutte, I., … Russinova, E. (2020). Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>","ista":"Liu D, Kumar R, LAN C, Johnson AJ, Siao W, Vanhoutte I, Wang P, Bender K, Yperman K, Martins S, Zhao X, Vert G, Van Damme D, Friml J, Russinova E. 2020. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. Plant Cell. 32(11), 3598–3612.","mla":"Liu, D., et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>, vol. 32, no. 11, American Society of Plant Biologists, 2020, pp. 3598–612, doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>."},"intvolume":"        32","oa":1,"publication_status":"published","main_file_link":[{"url":"https://europepmc.org/article/MED/32958564","open_access":"1"}],"date_published":"2020-11-01T00:00:00Z","year":"2020","_id":"8607","month":"11","type":"journal_article","oa_version":"Published Version","date_updated":"2023-09-05T12:21:32Z","abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles through the recognition of motifs based on tyrosine or di-leucine in their cytoplasmic tails. However, in plants, very little is known on how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis thaliana, the brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), undergoes endocytosis that depends on clathrin and AP-2. Here we demonstrate that BRI1 binds directly to the medium AP-2 subunit, AP2M. The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed tyrosine-based endocytic motifs. The tyrosine-to-phenylalanine substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional tyrosine motifs also operates in plants."}],"page":"3598-3612","date_created":"2020-10-05T12:45:16Z","volume":32}]