---
_id: '14726'
abstract:
- lang: eng
  text: Autocrine signaling pathways regulated by RAPID ALKALINIZATION FACTORs (RALFs)
    control cell wall integrity during pollen tube germination and growth in Arabidopsis
    (Arabidopsis thaliana). To investigate the role of pollen-specific RALFs in another
    plant species, we combined gene expression data with phylogenetic and biochemical
    studies to identify candidate orthologs in maize (Zea mays). We show that Clade
    IB ZmRALF2/3 mutations, but not Clade III ZmRALF1/5 mutations, cause cell wall
    instability in the sub-apical region of the growing pollen tube. ZmRALF2/3 are
    mainly located in the cell wall and are partially able to complement the pollen
    germination defect of their Arabidopsis orthologs AtRALF4/19. Mutations in ZmRALF2/3
    compromise pectin distribution patterns leading to altered cell wall organization
    and thickness culminating in pollen tube burst. Clade IB, but not Clade III ZmRALFs,
    strongly interact as ligands with the pollen-specific Catharanthus roseus RLK1-like
    (CrRLK1L) receptor kinases Zea mays FERONIA-like (ZmFERL) 4/7/9, LORELEI-like
    glycosylphosphatidylinositol-anchor (LLG) proteins Zea mays LLG 1 and 2 (ZmLLG1/2)
    and Zea mays pollen extension-like (PEX) cell wall proteins ZmPEX2/4. Notably,
    ZmFERL4 outcompetes ZmLLG2 and ZmPEX2 outcompetes ZmFERL4 for ZmRALF2 binding.
    Based on these data, we suggest that Clade IB RALFs act in a dual role as cell
    wall components and extracellular sensors to regulate cell wall integrity and
    thickness during pollen tube growth in maize and probably other plants.
article_number: koad324
article_processing_charge: No
article_type: original
author:
- first_name: Liang-Zi
  full_name: Zhou, Liang-Zi
  last_name: Zhou
- first_name: Lele
  full_name: Wang, Lele
  last_name: Wang
- first_name: Xia
  full_name: Chen, Xia
  last_name: Chen
- first_name: Zengxiang
  full_name: Ge, Zengxiang
  id: f43371a3-09ff-11eb-8013-bd0c6a2f6de8
  last_name: Ge
  orcid: 0000-0001-9381-3577
- first_name: Julia
  full_name: Mergner, Julia
  last_name: Mergner
- first_name: Xingli
  full_name: Li, Xingli
  last_name: Li
- first_name: Bernhard
  full_name: Küster, Bernhard
  last_name: Küster
- first_name: Gernot
  full_name: Längst, Gernot
  last_name: Längst
- first_name: Li-Jia
  full_name: Qu, Li-Jia
  last_name: Qu
- first_name: Thomas
  full_name: Dresselhaus, Thomas
  last_name: Dresselhaus
citation:
  ama: Zhou L-Z, Wang L, Chen X, et al. The RALF signaling pathway regulates cell
    wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. 2023.
    doi:<a href="https://doi.org/10.1093/plcell/koad324">10.1093/plcell/koad324</a>
  apa: Zhou, L.-Z., Wang, L., Chen, X., Ge, Z., Mergner, J., Li, X., … Dresselhaus,
    T. (2023). The RALF signaling pathway regulates cell wall integrity during pollen
    tube growth in maize. <i>The Plant Cell</i>. Oxford University Press. <a href="https://doi.org/10.1093/plcell/koad324">https://doi.org/10.1093/plcell/koad324</a>
  chicago: Zhou, Liang-Zi, Lele Wang, Xia Chen, Zengxiang Ge, Julia Mergner, Xingli
    Li, Bernhard Küster, Gernot Längst, Li-Jia Qu, and Thomas Dresselhaus. “The RALF
    Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.”
    <i>The Plant Cell</i>. Oxford University Press, 2023. <a href="https://doi.org/10.1093/plcell/koad324">https://doi.org/10.1093/plcell/koad324</a>.
  ieee: L.-Z. Zhou <i>et al.</i>, “The RALF signaling pathway regulates cell wall
    integrity during pollen tube growth in maize,” <i>The Plant Cell</i>. Oxford University
    Press, 2023.
  ista: Zhou L-Z, Wang L, Chen X, Ge Z, Mergner J, Li X, Küster B, Längst G, Qu L-J,
    Dresselhaus T. 2023. The RALF signaling pathway regulates cell wall integrity
    during pollen tube growth in maize. The Plant Cell., koad324.
  mla: Zhou, Liang-Zi, et al. “The RALF Signaling Pathway Regulates Cell Wall Integrity
    during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>, koad324, Oxford University
    Press, 2023, doi:<a href="https://doi.org/10.1093/plcell/koad324">10.1093/plcell/koad324</a>.
  short: L.-Z. Zhou, L. Wang, X. Chen, Z. Ge, J. Mergner, X. Li, B. Küster, G. Längst,
    L.-J. Qu, T. Dresselhaus, The Plant Cell (2023).
date_created: 2024-01-02T11:19:37Z
date_published: 2023-12-23T00:00:00Z
date_updated: 2024-01-03T12:43:41Z
day: '23'
ddc:
- '580'
doi: 10.1093/plcell/koad324
extern: '1'
has_accepted_license: '1'
keyword:
- Cell Biology
- Plant Science
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1093/plcell/koad324
month: '12'
oa: 1
oa_version: Published Version
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: epub_ahead
publisher: Oxford University Press
quality_controlled: '1'
status: public
title: The RALF signaling pathway regulates cell wall integrity during pollen tube
  growth in maize
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2023'
...
---
_id: '12669'
abstract:
- lang: eng
  text: The study of RNAs has become one of the most influential research fields in
    contemporary biology and biomedicine. In the last few years, new sequencing technologies
    have produced an explosion of new and exciting discoveries in the field but have
    also given rise to many open questions. Defining these questions, together with
    old, long-standing gaps in our knowledge, is the spirit of this article. The breadth
    of topics within RNA biology research is vast, and every aspect of the biology
    of these molecules contains countless exciting open questions. Here, we asked
    12 groups to discuss their most compelling question among some plant RNA biology
    topics. The following vignettes cover RNA alternative splicing; RNA dynamics;
    RNA translation; RNA structures; R-loops; epitranscriptomics; long non-coding
    RNAs; small RNA production and their functions in crops; small RNAs during gametogenesis
    and in cross-kingdom RNA interference; and RNA-directed DNA methylation. In each
    section, we will present the current state-of-the-art in plant RNA biology research
    before asking the questions that will surely motivate future discoveries in the
    field. We hope this article will spark a debate about the future perspective on
    RNA biology and provoke novel reflections in the reader.
article_number: koac346
article_processing_charge: No
article_type: original
author:
- first_name: Pablo A
  full_name: Manavella, Pablo A
  last_name: Manavella
- first_name: Micaela A
  full_name: Godoy Herz, Micaela A
  last_name: Godoy Herz
- first_name: Alberto R
  full_name: Kornblihtt, Alberto R
  last_name: Kornblihtt
- first_name: Reed
  full_name: Sorenson, Reed
  last_name: Sorenson
- first_name: Leslie E
  full_name: Sieburth, Leslie E
  last_name: Sieburth
- first_name: Kentaro
  full_name: Nakaminami, Kentaro
  last_name: Nakaminami
- first_name: Motoaki
  full_name: Seki, Motoaki
  last_name: Seki
- first_name: Yiliang
  full_name: Ding, Yiliang
  last_name: Ding
- first_name: Qianwen
  full_name: Sun, Qianwen
  last_name: Sun
- first_name: Hunseung
  full_name: Kang, Hunseung
  last_name: Kang
- first_name: Federico D
  full_name: Ariel, Federico D
  last_name: Ariel
- first_name: Martin
  full_name: Crespi, Martin
  last_name: Crespi
- first_name: Axel J
  full_name: Giudicatti, Axel J
  last_name: Giudicatti
- first_name: Qiang
  full_name: Cai, Qiang
  last_name: Cai
- first_name: Hailing
  full_name: Jin, Hailing
  last_name: Jin
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
- first_name: Yijun
  full_name: Qi, Yijun
  last_name: Qi
- first_name: Craig S
  full_name: Pikaard, Craig S
  last_name: Pikaard
citation:
  ama: 'Manavella PA, Godoy Herz MA, Kornblihtt AR, et al. Beyond transcription: compelling
    open questions in plant RNA biology. <i>The Plant Cell</i>. 2023;35(6). doi:<a
    href="https://doi.org/10.1093/plcell/koac346">10.1093/plcell/koac346</a>'
  apa: 'Manavella, P. A., Godoy Herz, M. A., Kornblihtt, A. R., Sorenson, R., Sieburth,
    L. E., Nakaminami, K., … Pikaard, C. S. (2023). Beyond transcription: compelling
    open questions in plant RNA biology. <i>The Plant Cell</i>. Oxford University
    Press. <a href="https://doi.org/10.1093/plcell/koac346">https://doi.org/10.1093/plcell/koac346</a>'
  chicago: 'Manavella, Pablo A, Micaela A Godoy Herz, Alberto R Kornblihtt, Reed Sorenson,
    Leslie E Sieburth, Kentaro Nakaminami, Motoaki Seki, et al. “Beyond Transcription:
    Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>. Oxford
    University Press, 2023. <a href="https://doi.org/10.1093/plcell/koac346">https://doi.org/10.1093/plcell/koac346</a>.'
  ieee: 'P. A. Manavella <i>et al.</i>, “Beyond transcription: compelling open questions
    in plant RNA biology,” <i>The Plant Cell</i>, vol. 35, no. 6. Oxford University
    Press, 2023.'
  ista: 'Manavella PA, Godoy Herz MA, Kornblihtt AR, Sorenson R, Sieburth LE, Nakaminami
    K, Seki M, Ding Y, Sun Q, Kang H, Ariel FD, Crespi M, Giudicatti AJ, Cai Q, Jin
    H, Feng X, Qi Y, Pikaard CS. 2023. Beyond transcription: compelling open questions
    in plant RNA biology. The Plant Cell. 35(6), koac346.'
  mla: 'Manavella, Pablo A., et al. “Beyond Transcription: Compelling Open Questions
    in Plant RNA Biology.” <i>The Plant Cell</i>, vol. 35, no. 6, koac346, Oxford
    University Press, 2023, doi:<a href="https://doi.org/10.1093/plcell/koac346">10.1093/plcell/koac346</a>.'
  short: P.A. Manavella, M.A. Godoy Herz, A.R. Kornblihtt, R. Sorenson, L.E. Sieburth,
    K. Nakaminami, M. Seki, Y. Ding, Q. Sun, H. Kang, F.D. Ariel, M. Crespi, A.J.
    Giudicatti, Q. Cai, H. Jin, X. Feng, Y. Qi, C.S. Pikaard, The Plant Cell 35 (2023).
date_created: 2023-02-23T09:14:59Z
date_published: 2023-06-01T00:00:00Z
date_updated: 2023-10-04T09:48:43Z
day: '01'
department:
- _id: XiFe
doi: 10.1093/plcell/koac346
extern: '1'
external_id:
  pmid:
  - '36477566'
intvolume: '        35'
issue: '6'
keyword:
- Cell Biology
- Plant Science
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1093/plcell/koac346
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Beyond transcription: compelling open questions in plant RNA biology'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 35
year: '2023'
...
---
_id: '12053'
abstract:
- lang: eng
  text: Strigolactones (SLs) are a class of phytohormones that regulate plant shoot
    branching and adventitious root development. However, little is known regarding
    the role of SLs in controlling the behavior of the smallest unit of the organism,
    the single cell. Here, taking advantage of a classic single-cell model offered
    by the cotton (Gossypium hirsutum) fiber cell, we show that SLs, whose biosynthesis
    is fine-tuned by gibberellins (GAs), positively regulate cell elongation and cell
    wall thickness by promoting the biosynthesis of very-long-chain fatty acids (VLCFAs)
    and cellulose, respectively. Furthermore, we identified two layers of transcription
    factors (TFs) involved in the hierarchical regulation of this GA-SL crosstalk.
    The top-layer TF GROWTH-REGULATING FACTOR 4 (GhGRF4) directly activates expression
    of the SL biosynthetic gene DWARF27 (D27) to increase SL accumulation in fiber
    cells and GAs induce GhGRF4 expression. SLs induce the expression of four second-layer
    TF genes (GhNAC100-2, GhBLH51, GhGT2, and GhB9SHZ1), which transmit SL signals
    downstream to two ketoacyl-CoA synthase genes (KCS) and three cellulose synthase
    (CesA) genes by directly activating their transcription. Finally, the KCS and
    CesA enzymes catalyze the biosynthesis of very long chain fatty acids and cellulose,
    respectively, to regulate development of high-grade cotton fibers. In addition
    to providing a theoretical basis for cotton fiber improvement, our results shed
    light on SL signaling in plant development at the single-cell level.
acknowledgement: This work was supported by the National Natural Science Foundation
  of China (32070549), Shaanxi Youth Entrusted Talent Program (20190205), Fundamental
  Research Funds for the Central Universities (GK202002005 and GK202201017), Young
  Elite Scientists Sponsorship Program by China Association for Science and Technology
  (CAST) (2019-2021QNRC001), State Key Laboratory of Cotton Biology Open Fund (CB2020A12
  and CB2021A21) and FWF Stand-alone Project (P29988).
article_processing_charge: No
article_type: original
author:
- first_name: Z
  full_name: Tian, Z
  last_name: Tian
- first_name: Yuzhou
  full_name: Zhang, Yuzhou
  id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
  last_name: Zhang
  orcid: 0000-0003-2627-6956
- first_name: L
  full_name: Zhu, L
  last_name: Zhu
- first_name: B
  full_name: Jiang, B
  last_name: Jiang
- first_name: H
  full_name: Wang, H
  last_name: Wang
- first_name: R
  full_name: Gao, R
  last_name: Gao
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: G
  full_name: Xiao, G
  last_name: Xiao
citation:
  ama: Tian Z, Zhang Y, Zhu L, et al. Strigolactones act downstream of gibberellins
    to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium
    hirsutum). <i>The Plant Cell</i>. 2022;34(12):4816-4839. doi:<a href="https://doi.org/10.1093/plcell/koac270">10.1093/plcell/koac270</a>
  apa: Tian, Z., Zhang, Y., Zhu, L., Jiang, B., Wang, H., Gao, R., … Xiao, G. (2022).
    Strigolactones act downstream of gibberellins to regulate fiber cell elongation
    and cell wall thickness in cotton (Gossypium hirsutum). <i>The Plant Cell</i>.
    Oxford University Press. <a href="https://doi.org/10.1093/plcell/koac270">https://doi.org/10.1093/plcell/koac270</a>
  chicago: Tian, Z, Yuzhou Zhang, L Zhu, B Jiang, H Wang, R Gao, Jiří Friml, and G
    Xiao. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation
    and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” <i>The Plant Cell</i>.
    Oxford University Press, 2022. <a href="https://doi.org/10.1093/plcell/koac270">https://doi.org/10.1093/plcell/koac270</a>.
  ieee: Z. Tian <i>et al.</i>, “Strigolactones act downstream of gibberellins to regulate
    fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum),”
    <i>The Plant Cell</i>, vol. 34, no. 12. Oxford University Press, pp. 4816–4839,
    2022.
  ista: Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R, Friml J, Xiao G. 2022. Strigolactones
    act downstream of gibberellins to regulate fiber cell elongation and cell wall
    thickness in cotton (Gossypium hirsutum). The Plant Cell. 34(12), 4816–4839.
  mla: Tian, Z., et al. “Strigolactones Act Downstream of Gibberellins to Regulate
    Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).”
    <i>The Plant Cell</i>, vol. 34, no. 12, Oxford University Press, 2022, pp. 4816–39,
    doi:<a href="https://doi.org/10.1093/plcell/koac270">10.1093/plcell/koac270</a>.
  short: Z. Tian, Y. Zhang, L. Zhu, B. Jiang, H. Wang, R. Gao, J. Friml, G. Xiao,
    The Plant Cell 34 (2022) 4816–4839.
date_created: 2022-09-07T14:19:39Z
date_published: 2022-12-01T00:00:00Z
date_updated: 2023-08-03T13:41:06Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1093/plcell/koac270
external_id:
  isi:
  - '000852753000001'
  pmid:
  - '36040191'
file:
- access_level: open_access
  checksum: 1c606d9545f29dfca15235f69ad27b58
  content_type: application/pdf
  creator: dernst
  date_created: 2023-01-20T08:29:12Z
  date_updated: 2023-01-20T08:29:12Z
  file_id: '12318'
  file_name: 2022_PlantCell_Tian.pdf
  file_size: 3282540
  relation: main_file
  success: 1
file_date_updated: 2023-01-20T08:29:12Z
has_accepted_license: '1'
intvolume: '        34'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: 4816-4839
pmid: 1
project:
- _id: 262EF96E-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: P29988
  name: RNA-directed DNA methylation in plant development
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
  link:
  - relation: erratum
    url: https://doi.org/10.1093/plcell/koac342
scopus_import: '1'
status: public
title: Strigolactones act downstream of gibberellins to regulate fiber cell elongation
  and cell wall thickness in cotton (Gossypium hirsutum)
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 34
year: '2022'
...
---
_id: '7619'
abstract:
- lang: eng
  text: Cell polarity is a fundamental feature of all multicellular organisms. In
    plants, prominent cell polarity markers are PIN auxin transporters crucial for
    plant development. To identify novel components involved in cell polarity establishment
    and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2
    Arabidopsis plants, which ectopically express predominantly basally localized
    PIN1 in the root epidermal cells leading to agravitropic root growth. From the
    screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which
    restored gravitropic root growth and caused PIN1-HA polarity switch from basal
    to apical side of root epidermal cells. Complementation experiments established
    the repp12 causative mutation as an amino acid substitution in Aminophospholipid
    ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation.
    ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric
    auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple
    mutants confirmed a crucial role of ALA proteins in regulating plant development
    and in PIN trafficking and polarity. Genetic and physical interaction studies
    revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together,
    our results identified ALA3 flippase as an important interactor and regulator
    of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development.
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
article_type: original
author:
- first_name: Xixi
  full_name: Zhang, Xixi
  id: 61A66458-47E9-11EA-85BA-8AEAAF14E49A
  last_name: Zhang
  orcid: 0000-0001-7048-4627
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Petra
  full_name: Marhavá, Petra
  id: 44E59624-F248-11E8-B48F-1D18A9856A87
  last_name: Marhavá
- first_name: Shutang
  full_name: Tan, Shutang
  id: 2DE75584-F248-11E8-B48F-1D18A9856A87
  last_name: Tan
  orcid: 0000-0002-0471-8285
- first_name: Yuzhou
  full_name: Zhang, Yuzhou
  id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
  last_name: Zhang
  orcid: 0000-0003-2627-6956
- first_name: Lesia
  full_name: Rodriguez Solovey, Lesia
  id: 3922B506-F248-11E8-B48F-1D18A9856A87
  last_name: Rodriguez Solovey
  orcid: 0000-0002-7244-7237
- first_name: Marta
  full_name: Zwiewka, Marta
  last_name: Zwiewka
- first_name: Vendula
  full_name: Pukyšová, Vendula
  last_name: Pukyšová
- first_name: Adrià Sans
  full_name: Sánchez, Adrià Sans
  last_name: Sánchez
- first_name: Vivek Kumar
  full_name: Raxwal, Vivek Kumar
  last_name: Raxwal
- first_name: Christian S.
  full_name: Hardtke, Christian S.
  last_name: Hardtke
- first_name: Tomasz
  full_name: Nodzynski, Tomasz
  last_name: Nodzynski
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Zhang X, Adamowski M, Marhavá P, et al. Arabidopsis flippases cooperate with
    ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin
    transporters. <i>The Plant Cell</i>. 2020;32(5):1644-1664. doi:<a href="https://doi.org/10.1105/tpc.19.00869">10.1105/tpc.19.00869</a>
  apa: Zhang, X., Adamowski, M., Marhavá, P., Tan, S., Zhang, Y., Rodriguez Solovey,
    L., … Friml, J. (2020). Arabidopsis flippases cooperate with ARF GTPase exchange
    factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The
    Plant Cell</i>. American Society of Plant Biologists. <a href="https://doi.org/10.1105/tpc.19.00869">https://doi.org/10.1105/tpc.19.00869</a>
  chicago: Zhang, Xixi, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang,
    Lesia Rodriguez Solovey, Marta Zwiewka, et al. “Arabidopsis Flippases Cooperate
    with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN
    Auxin Transporters.” <i>The Plant Cell</i>. American Society of Plant Biologists,
    2020. <a href="https://doi.org/10.1105/tpc.19.00869">https://doi.org/10.1105/tpc.19.00869</a>.
  ieee: X. Zhang <i>et al.</i>, “Arabidopsis flippases cooperate with ARF GTPase exchange
    factors to regulate the trafficking and polarity of PIN auxin transporters,” <i>The
    Plant Cell</i>, vol. 32, no. 5. American Society of Plant Biologists, pp. 1644–1664,
    2020.
  ista: Zhang X, Adamowski M, Marhavá P, Tan S, Zhang Y, Rodriguez Solovey L, Zwiewka
    M, Pukyšová V, Sánchez AS, Raxwal VK, Hardtke CS, Nodzynski T, Friml J. 2020.
    Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the
    trafficking and polarity of PIN auxin transporters. The Plant Cell. 32(5), 1644–1664.
  mla: Zhang, Xixi, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange
    Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The
    Plant Cell</i>, vol. 32, no. 5, American Society of Plant Biologists, 2020, pp.
    1644–64, doi:<a href="https://doi.org/10.1105/tpc.19.00869">10.1105/tpc.19.00869</a>.
  short: X. Zhang, M. Adamowski, P. Marhavá, S. Tan, Y. Zhang, L. Rodriguez Solovey,
    M. Zwiewka, V. Pukyšová, A.S. Sánchez, V.K. Raxwal, C.S. Hardtke, T. Nodzynski,
    J. Friml, The Plant Cell 32 (2020) 1644–1664.
date_created: 2020-03-28T07:39:22Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2023-09-05T12:21:06Z
day: '01'
department:
- _id: JiFr
doi: 10.1105/tpc.19.00869
ec_funded: 1
external_id:
  isi:
  - '000545741500030'
  pmid:
  - '32193204'
intvolume: '        32'
isi: 1
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1105/tpc.19.00869
month: '05'
oa: 1
oa_version: Published Version
page: 1644-1664
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate
  the trafficking and polarity of PIN auxin transporters
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 32
year: '2020'
...
---
_id: '412'
abstract:
- lang: eng
  text: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which
    cargoes and lipids are internalized from the plasma membrane into vesicles coated
    with clathrin and adaptor proteins. CME is essential for many developmental and
    physiological processes in plants, but its underlying mechanism is not well characterised
    compared to that in yeast and animal systems. Here, we searched for new factors
    involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification
    of proteins that interact with clathrin light chain, a principal component of
    the clathrin coat. Among the confirmed interactors, we found two putative homologues
    of the clathrin-coat uncoating factor auxilin previously described in non-plant
    systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused
    an arrest of seedling growth and development. This was concomitant with inhibited
    endocytosis due to blocking of clathrin recruitment after the initial step of
    adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2)
    loss-of-function lines did not present endocytosis-related developmental or cellular
    phenotypes under normal growth conditions. This work contributes to the on-going
    characterization of the endocytotic machinery in plants and provides a robust
    tool for conditionally and specifically interfering with CME in A. thaliana.
acknowledgement: We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner
  at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9
  construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek,
  Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych,
  Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for
  help with correcting the manuscript. This work was supported by the European Research
  Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC
  Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project
  NPUI-LO1417.
article_processing_charge: No
article_type: original
author:
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Madhumitha
  full_name: Narasimhan, Madhumitha
  id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
  last_name: Narasimhan
  orcid: 0000-0002-8600-0671
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Geert
  full_name: De Jaeger, Geert
  last_name: De Jaeger
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional
    study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis.
    <i>The Plant Cell</i>. 2018;30(3):700-716. doi:<a href="https://doi.org/10.1105/tpc.17.00785">10.1105/tpc.17.00785</a>
  apa: Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., &#38; Friml,
    J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating
    factors in Arabidopsis. <i>The Plant Cell</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1105/tpc.17.00785">https://doi.org/10.1105/tpc.17.00785</a>
  chicago: Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc,
    Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two
    Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>. American
    Society of Plant Biologists, 2018. <a href="https://doi.org/10.1105/tpc.17.00785">https://doi.org/10.1105/tpc.17.00785</a>.
  ieee: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml,
    “A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
    in Arabidopsis,” <i>The Plant Cell</i>, vol. 30, no. 3. American Society of Plant
    Biologists, pp. 700–716, 2018.
  ista: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A
    functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
    in Arabidopsis. The Plant Cell. 30(3), 700–716.
  mla: Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative
    Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>, vol. 30, no.
    3, American Society of Plant Biologists, 2018, pp. 700–16, doi:<a href="https://doi.org/10.1105/tpc.17.00785">10.1105/tpc.17.00785</a>.
  short: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml,
    The Plant Cell 30 (2018) 700–716.
date_created: 2018-12-11T11:46:20Z
date_published: 2018-04-09T00:00:00Z
date_updated: 2025-05-07T11:12:27Z
day: '09'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.17.00785
ec_funded: 1
external_id:
  isi:
  - '000429441400018'
  pmid:
  - '29511054'
file:
- access_level: open_access
  checksum: 4e165e653b67d3f0684697f21aace5a1
  content_type: application/pdf
  creator: dernst
  date_created: 2022-05-23T09:12:38Z
  date_updated: 2022-05-23T09:12:38Z
  file_id: '11406'
  file_name: 2018_PlantCell_Adamowski.pdf
  file_size: 4407538
  relation: main_file
  success: 1
file_date_updated: 2022-05-23T09:12:38Z
has_accepted_license: '1'
intvolume: '        30'
isi: 1
issue: '3'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
page: 700 - 716
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7417'
quality_controlled: '1'
related_material:
  record:
  - id: '6269'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
  in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
year: '2018'
...