---
_id: '9794'
abstract:
- lang: eng
  text: 'Lymph nodes (LNs) comprise two main structural elements: fibroblastic reticular
    cells that form dedicated niches for immune cell interaction and capsular fibroblasts
    that build a shell around the organ. Immunological challenge causes LNs to increase
    more than tenfold in size within a few days. Here, we characterized the biomechanics
    of LN swelling on the cellular and organ scale. We identified lymphocyte trapping
    by influx and proliferation as drivers of an outward pressure force, causing fibroblastic
    reticular cells of the T-zone (TRCs) and their associated conduits to stretch.
    After an initial phase of relaxation, TRCs sensed the resulting strain through
    cell matrix adhesions, which coordinated local growth and remodeling of the stromal
    network. While the expanded TRC network readopted its typical configuration, a
    massive fibrotic reaction of the organ capsule set in and countered further organ
    expansion. Thus, different fibroblast populations mechanically control LN swelling
    in a multitier fashion.'
acknowledged_ssus:
- _id: Bio
- _id: EM-Fac
- _id: PreCl
- _id: LifeSc
acknowledgement: This research was supported by the Scientific Service Units of IST
  Austria through resources provided by the Imaging and Optics, Electron Microscopy,
  Preclinical and Life Science Facilities. We thank C. Moussion for providing anti-PNAd
  antibody and D. Critchley for Talin1-floxed mice, and E. Papusheva for providing
  a custom 3D channel alignment script. This work was supported by a European Research
  Council grant ERC-CoG-72437 to M.S. M.H. was supported by Czech Sciencundation GACR
  20-24603Y and Charles University PRIMUS/20/MED/013.
article_processing_charge: No
article_type: original
author:
- first_name: Frank P
  full_name: Assen, Frank P
  id: 3A8E7F24-F248-11E8-B48F-1D18A9856A87
  last_name: Assen
  orcid: 0000-0003-3470-6119
- first_name: Jun
  full_name: Abe, Jun
  last_name: Abe
- first_name: Miroslav
  full_name: Hons, Miroslav
  id: 4167FE56-F248-11E8-B48F-1D18A9856A87
  last_name: Hons
  orcid: 0000-0002-6625-3348
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Shayan
  full_name: Shamipour, Shayan
  id: 40B34FE2-F248-11E8-B48F-1D18A9856A87
  last_name: Shamipour
- first_name: Walter
  full_name: Kaufmann, Walter
  id: 3F99E422-F248-11E8-B48F-1D18A9856A87
  last_name: Kaufmann
  orcid: 0000-0001-9735-5315
- first_name: Tommaso
  full_name: Costanzo, Tommaso
  id: D93824F4-D9BA-11E9-BB12-F207E6697425
  last_name: Costanzo
  orcid: 0000-0001-9732-3815
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Markus
  full_name: Brown, Markus
  id: 3DAB9AFC-F248-11E8-B48F-1D18A9856A87
  last_name: Brown
- first_name: Burkhard
  full_name: Ludewig, Burkhard
  last_name: Ludewig
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Wolfgang
  full_name: Weninger, Wolfgang
  last_name: Weninger
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Sanjiv A.
  full_name: Luther, Sanjiv A.
  last_name: Luther
- first_name: Jens V.
  full_name: Stein, Jens V.
  last_name: Stein
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-4561-241X
citation:
  ama: Assen FP, Abe J, Hons M, et al. Multitier mechanics control stromal adaptations
    in swelling lymph nodes. <i>Nature Immunology</i>. 2022;23:1246-1255. doi:<a href="https://doi.org/10.1038/s41590-022-01257-4">10.1038/s41590-022-01257-4</a>
  apa: Assen, F. P., Abe, J., Hons, M., Hauschild, R., Shamipour, S., Kaufmann, W.,
    … Sixt, M. K. (2022). Multitier mechanics control stromal adaptations in swelling
    lymph nodes. <i>Nature Immunology</i>. Springer Nature. <a href="https://doi.org/10.1038/s41590-022-01257-4">https://doi.org/10.1038/s41590-022-01257-4</a>
  chicago: Assen, Frank P, Jun Abe, Miroslav Hons, Robert Hauschild, Shayan Shamipour,
    Walter Kaufmann, Tommaso Costanzo, et al. “Multitier Mechanics Control Stromal
    Adaptations in Swelling Lymph Nodes.” <i>Nature Immunology</i>. Springer Nature,
    2022. <a href="https://doi.org/10.1038/s41590-022-01257-4">https://doi.org/10.1038/s41590-022-01257-4</a>.
  ieee: F. P. Assen <i>et al.</i>, “Multitier mechanics control stromal adaptations
    in swelling lymph nodes,” <i>Nature Immunology</i>, vol. 23. Springer Nature,
    pp. 1246–1255, 2022.
  ista: Assen FP, Abe J, Hons M, Hauschild R, Shamipour S, Kaufmann W, Costanzo T,
    Krens G, Brown M, Ludewig B, Hippenmeyer S, Heisenberg C-PJ, Weninger W, Hannezo
    EB, Luther SA, Stein JV, Sixt MK. 2022. Multitier mechanics control stromal adaptations
    in swelling lymph nodes. Nature Immunology. 23, 1246–1255.
  mla: Assen, Frank P., et al. “Multitier Mechanics Control Stromal Adaptations in
    Swelling Lymph Nodes.” <i>Nature Immunology</i>, vol. 23, Springer Nature, 2022,
    pp. 1246–55, doi:<a href="https://doi.org/10.1038/s41590-022-01257-4">10.1038/s41590-022-01257-4</a>.
  short: F.P. Assen, J. Abe, M. Hons, R. Hauschild, S. Shamipour, W. Kaufmann, T.
    Costanzo, G. Krens, M. Brown, B. Ludewig, S. Hippenmeyer, C.-P.J. Heisenberg,
    W. Weninger, E.B. Hannezo, S.A. Luther, J.V. Stein, M.K. Sixt, Nature Immunology
    23 (2022) 1246–1255.
date_created: 2021-08-06T09:09:11Z
date_published: 2022-07-11T00:00:00Z
date_updated: 2023-08-02T06:53:07Z
day: '11'
ddc:
- '570'
department:
- _id: SiHi
- _id: CaHe
- _id: EdHa
- _id: EM-Fac
- _id: Bio
- _id: MiSi
doi: 10.1038/s41590-022-01257-4
ec_funded: 1
external_id:
  isi:
  - '000822975900002'
file:
- access_level: open_access
  checksum: 628e7b49809f22c75b428842efe70c68
  content_type: application/pdf
  creator: dernst
  date_created: 2022-07-25T07:11:32Z
  date_updated: 2022-07-25T07:11:32Z
  file_id: '11642'
  file_name: 2022_NatureImmunology_Assen.pdf
  file_size: 11475325
  relation: main_file
  success: 1
file_date_updated: 2022-07-25T07:11:32Z
has_accepted_license: '1'
intvolume: '        23'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '07'
oa: 1
oa_version: Published Version
page: 1246-1255
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '724373'
  name: Cellular navigation along spatial gradients
publication: Nature Immunology
publication_identifier:
  eissn:
  - 1529-2916
  issn:
  - 1529-2908
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: Multitier mechanics control stromal adaptations in swelling lymph nodes
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 23
year: '2022'
...