---
_id: '9952'
abstract:
- lang: eng
  text: Proper control of division orientation and symmetry, largely determined by
    spindle positioning, is essential to development and homeostasis. Spindle positioning
    has been extensively studied in cells dividing in two-dimensional (2D) environments
    and in epithelial tissues, where proteins such as NuMA (also known as NUMA1) orient
    division along the interphase long axis of the cell. However, little is known
    about how cells control spindle positioning in three-dimensional (3D) environments,
    such as early mammalian embryos and a variety of adult tissues. Here, we use mouse
    embryonic stem cells (ESCs), which grow in 3D colonies, as a model to investigate
    division in 3D. We observe that, at the periphery of 3D colonies, ESCs display
    high spindle mobility and divide asymmetrically. Our data suggest that enhanced
    spindle movements are due to unequal distribution of the cell–cell junction protein
    E-cadherin between future daughter cells. Interestingly, when cells progress towards
    differentiation, division becomes more symmetric, with more elongated shapes in
    metaphase and enhanced cortical NuMA recruitment in anaphase. Altogether, this
    study suggests that in 3D contexts, the geometry of the cell and its contacts
    with neighbors control division orientation and symmetry.
acknowledgement: We would like to thank the entire Paluch and Baum laboratories at
  the MRC-LMCB and the Chalut lab at the Cambridge SCI for discussions and feedback
  throughout the project, and the MRC-LMCB microscopy platform, in particular Andrew
  Vaughan, for technical support.
article_number: jcs255018
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Agathe
  full_name: Chaigne, Agathe
  last_name: Chaigne
- first_name: Matthew B.
  full_name: Smith, Matthew B.
  last_name: Smith
- first_name: R. L.
  full_name: Cavestany, R. L.
  last_name: Cavestany
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Kevin J.
  full_name: Chalut, Kevin J.
  last_name: Chalut
- first_name: Ewa K.
  full_name: Paluch, Ewa K.
  last_name: Paluch
citation:
  ama: Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. Three-dimensional
    geometry controls division symmetry in stem cell colonies. <i>Journal of Cell
    Science</i>. 2021;134(14). doi:<a href="https://doi.org/10.1242/jcs.255018">10.1242/jcs.255018</a>
  apa: Chaigne, A., Smith, M. B., Cavestany, R. L., Hannezo, E. B., Chalut, K. J.,
    &#38; Paluch, E. K. (2021). Three-dimensional geometry controls division symmetry
    in stem cell colonies. <i>Journal of Cell Science</i>. The Company of Biologists.
    <a href="https://doi.org/10.1242/jcs.255018">https://doi.org/10.1242/jcs.255018</a>
  chicago: Chaigne, Agathe, Matthew B. Smith, R. L. Cavestany, Edouard B Hannezo,
    Kevin J. Chalut, and Ewa K. Paluch. “Three-Dimensional Geometry Controls Division
    Symmetry in Stem Cell Colonies.” <i>Journal of Cell Science</i>. The Company of
    Biologists, 2021. <a href="https://doi.org/10.1242/jcs.255018">https://doi.org/10.1242/jcs.255018</a>.
  ieee: A. Chaigne, M. B. Smith, R. L. Cavestany, E. B. Hannezo, K. J. Chalut, and
    E. K. Paluch, “Three-dimensional geometry controls division symmetry in stem cell
    colonies,” <i>Journal of Cell Science</i>, vol. 134, no. 14. The Company of Biologists,
    2021.
  ista: Chaigne A, Smith MB, Cavestany RL, Hannezo EB, Chalut KJ, Paluch EK. 2021.
    Three-dimensional geometry controls division symmetry in stem cell colonies. Journal
    of Cell Science. 134(14), jcs255018.
  mla: Chaigne, Agathe, et al. “Three-Dimensional Geometry Controls Division Symmetry
    in Stem Cell Colonies.” <i>Journal of Cell Science</i>, vol. 134, no. 14, jcs255018,
    The Company of Biologists, 2021, doi:<a href="https://doi.org/10.1242/jcs.255018">10.1242/jcs.255018</a>.
  short: A. Chaigne, M.B. Smith, R.L. Cavestany, E.B. Hannezo, K.J. Chalut, E.K. Paluch,
    Journal of Cell Science 134 (2021).
date_created: 2021-08-22T22:01:20Z
date_published: 2021-07-01T00:00:00Z
date_updated: 2023-08-11T10:55:36Z
day: '01'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1242/jcs.255018
external_id:
  isi:
  - '000681395800008'
file:
- access_level: open_access
  checksum: f086f9d7cb63b2474c01921cb060c513
  content_type: application/pdf
  creator: asandaue
  date_created: 2021-08-23T07:32:20Z
  date_updated: 2021-08-23T07:32:20Z
  file_id: '9954'
  file_name: 2021_JournalOfCellScience_Chaigne.pdf
  file_size: 8651724
  relation: main_file
  success: 1
file_date_updated: 2021-08-23T07:32:20Z
has_accepted_license: '1'
intvolume: '       134'
isi: 1
issue: '14'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
publication: Journal of Cell Science
publication_identifier:
  eissn:
  - '14779137'
  issn:
  - '00219533'
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Three-dimensional geometry controls division symmetry in stem cell colonies
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 134
year: '2021'
...