[{"oa":1,"publication_status":"published","main_file_link":[{"url":"https://doi.org/10.1101/2023.03.27.534325","open_access":"1"}],"date_published":"2023-09-01T00:00:00Z","status":"public","external_id":{"pmid":["37539494"]},"citation":{"ieee":"M. Nagano, K. Aoshima, H. Shimamura, D. E. Siekhaus, J. Y. Toshima, and J. Toshima, “Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway,” <i>Journal of Cell Science</i>, vol. 136, no. 17. The Company of Biologists, 2023.","chicago":"Nagano, Makoto, Kaito Aoshima, Hiroki Shimamura, Daria E Siekhaus, Junko Y. Toshima, and Jiro Toshima. “Distinct Role of TGN-Resident Clathrin Adaptors for Vps21p Activation in the TGN-Endosome Trafficking Pathway.” <i>Journal of Cell Science</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/jcs.261448\">https://doi.org/10.1242/jcs.261448</a>.","short":"M. Nagano, K. Aoshima, H. Shimamura, D.E. Siekhaus, J.Y. Toshima, J. Toshima, Journal of Cell Science 136 (2023).","ama":"Nagano M, Aoshima K, Shimamura H, Siekhaus DE, Toshima JY, Toshima J. Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway. <i>Journal of Cell Science</i>. 2023;136(17). doi:<a href=\"https://doi.org/10.1242/jcs.261448\">10.1242/jcs.261448</a>","ista":"Nagano M, Aoshima K, Shimamura H, Siekhaus DE, Toshima JY, Toshima J. 2023. Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway. Journal of Cell Science. 136(17), jcs261448.","mla":"Nagano, Makoto, et al. “Distinct Role of TGN-Resident Clathrin Adaptors for Vps21p Activation in the TGN-Endosome Trafficking Pathway.” <i>Journal of Cell Science</i>, vol. 136, no. 17, jcs261448, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/jcs.261448\">10.1242/jcs.261448</a>.","apa":"Nagano, M., Aoshima, K., Shimamura, H., Siekhaus, D. E., Toshima, J. Y., &#38; Toshima, J. (2023). Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.261448\">https://doi.org/10.1242/jcs.261448</a>"},"intvolume":"       136","abstract":[{"lang":"eng","text":"Clathrin-mediated vesicle trafficking plays central roles in post-Golgi transport. In yeast (Saccharomyces cerevisiae), the AP-1 complex and GGA adaptors are predicted to generate distinct transport vesicles at the trans-Golgi network (TGN), and the epsin-related proteins Ent3p and Ent5p (collectively Ent3p/5p) act as accessories for these adaptors. Recently, we showed that vesicle transport from the TGN is crucial for yeast Rab5 (Vps21p)-mediated endosome formation, and that Ent3p/5p are crucial for this process, whereas AP-1 and GGA adaptors are dispensable. However, these observations were incompatible with previous studies showing that these adaptors are required for Ent3p/5p recruitment to the TGN, and thus the overall mechanism responsible for regulation of Vps21p activity remains ambiguous. Here, we investigated the functional relationships between clathrin adaptors in post-Golgi-mediated Vps21p activation. We show that AP-1 disruption in the ent3Δ5Δ mutant impaired transport of the Vps21p guanine nucleotide exchange factor Vps9p transport to the Vps21p compartment and severely reduced Vps21p activity. Additionally, GGA adaptors, the phosphatidylinositol-4-kinase Pik1p and Rab11 GTPases Ypt31p and Ypt32p were found to have partially overlapping functions for recruitment of AP-1 and Ent3p/5p to the TGN. These findings suggest a distinct role of clathrin adaptors for Vps21p activation in the TGN–endosome trafficking pathway."}],"date_updated":"2023-09-20T09:14:15Z","oa_version":"Preprint","type":"journal_article","month":"09","date_created":"2023-09-10T22:01:12Z","volume":136,"year":"2023","_id":"14316","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"quality_controlled":"1","doi":"10.1242/jcs.261448","language":[{"iso":"eng"}],"issue":"17","day":"01","author":[{"full_name":"Nagano, Makoto","first_name":"Makoto","last_name":"Nagano"},{"last_name":"Aoshima","first_name":"Kaito","full_name":"Aoshima, Kaito"},{"last_name":"Shimamura","first_name":"Hiroki","full_name":"Shimamura, Hiroki"},{"orcid":"0000-0001-8323-8353","id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","full_name":"Siekhaus, Daria E","first_name":"Daria E","last_name":"Siekhaus"},{"first_name":"Junko Y.","last_name":"Toshima","full_name":"Toshima, Junko Y."},{"first_name":"Jiro","last_name":"Toshima","full_name":"Toshima, Jiro"}],"title":"Distinct role of TGN-resident clathrin adaptors for Vps21p activation in the TGN-endosome trafficking pathway","article_number":"jcs261448","pmid":1,"department":[{"_id":"DaSi"}],"publisher":"The Company of Biologists","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_processing_charge":"No","scopus_import":"1","article_type":"original","publication":"Journal of Cell Science"},{"author":[{"first_name":"Cornelia","last_name":"Schwayer","id":"3436488C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5130-2226","full_name":"Schwayer, Cornelia"},{"last_name":"Brückner","first_name":"David","full_name":"Brückner, David","orcid":"0000-0001-7205-2975","id":"e1e86031-6537-11eb-953a-f7ab92be508d"}],"day":"27","title":"Connecting theory and experiment in cell and tissue mechanics","article_number":"jcs.261515","publisher":"The Company of Biologists","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","pmid":1,"department":[{"_id":"EdHa"},{"_id":"CaHe"}],"publication":"Journal of Cell Science","article_processing_charge":"No","scopus_import":"1","article_type":"original","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"doi":"10.1242/jcs.261515","quality_controlled":"1","project":[{"grant_number":"343-2022","_id":"34e2a5b5-11ca-11ed-8bc3-b2265616ef0b","name":"A mechano-chemical theory for stem cell fate decisions in organoid development"}],"keyword":["Cell Biology"],"language":[{"iso":"eng"}],"issue":"24","date_updated":"2024-01-22T13:35:48Z","abstract":[{"text":"Understanding complex living systems, which are fundamentally constrained by physical phenomena, requires combining experimental data with theoretical physical and mathematical models. To develop such models, collaborations between experimental cell biologists and theoreticians are increasingly important but these two groups often face challenges achieving mutual understanding. To help navigate these challenges, this Perspective discusses different modelling approaches, including bottom-up hypothesis-driven and top-down data-driven models, and highlights their strengths and applications. Using cell mechanics as an example, we explore the integration of specific physical models with experimental data from the molecular, cellular and tissue level up to multiscale input. We also emphasize the importance of constraining model complexity and outline strategies for crosstalk between experimental design and model development. Furthermore, we highlight how physical models can provide conceptual insights and produce unifying and generalizable frameworks for biological phenomena. Overall, this Perspective aims to promote fruitful collaborations that advance our understanding of complex biological systems.","lang":"eng"}],"oa_version":"None","month":"12","type":"journal_article","volume":136,"date_created":"2024-01-17T12:46:55Z","acknowledgement":"We thank Prisca Liberali and Edouard Hannezo for many inspiring discussions; Mehmet Can Uçar, Nicoletta I Petridou and Qiutan Yang for a critical reading of the manuscript, and Claudia Flandoli for the artwork in Figs 2 and 3. We would also like to thank The Company of Biologists for the opportunity to attend the 2023 workshop on Collective Cell Migration, and all workshop participants for discussions.\r\nC.S. was supported by a European Molecular Biology Organization (EMBO) Postdoctoral Fellowship (ALTF 660-2020) and Human Frontier Science Program (HFSP) Postdoctoral fellowship (LT000746/2021-L). D.B.B. was supported by the NOMIS Foundation as a NOMIS Fellow and by an EMBO Postdoctoral Fellowship (ALTF 343-2022).","year":"2023","_id":"14827","publication_status":"published","date_published":"2023-12-27T00:00:00Z","external_id":{"pmid":["38149871"]},"status":"public","intvolume":"       136","citation":{"chicago":"Schwayer, Cornelia, and David Brückner. “Connecting Theory and Experiment in Cell and Tissue Mechanics.” <i>Journal of Cell Science</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/jcs.261515\">https://doi.org/10.1242/jcs.261515</a>.","ieee":"C. Schwayer and D. Brückner, “Connecting theory and experiment in cell and tissue mechanics,” <i>Journal of Cell Science</i>, vol. 136, no. 24. The Company of Biologists, 2023.","short":"C. Schwayer, D. Brückner, Journal of Cell Science 136 (2023).","ama":"Schwayer C, Brückner D. Connecting theory and experiment in cell and tissue mechanics. <i>Journal of Cell Science</i>. 2023;136(24). doi:<a href=\"https://doi.org/10.1242/jcs.261515\">10.1242/jcs.261515</a>","apa":"Schwayer, C., &#38; Brückner, D. (2023). Connecting theory and experiment in cell and tissue mechanics. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.261515\">https://doi.org/10.1242/jcs.261515</a>","ista":"Schwayer C, Brückner D. 2023. Connecting theory and experiment in cell and tissue mechanics. Journal of Cell Science. 136(24), jcs. 261515.","mla":"Schwayer, Cornelia, and David Brückner. “Connecting Theory and Experiment in Cell and Tissue Mechanics.” <i>Journal of Cell Science</i>, vol. 136, no. 24, jcs. 261515, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/jcs.261515\">10.1242/jcs.261515</a>."}},{"volume":136,"date_created":"2023-08-20T22:01:13Z","file_date_updated":"2023-08-21T07:37:54Z","type":"journal_article","oa_version":"None","month":"08","date_updated":"2023-12-13T12:11:18Z","abstract":[{"text":"Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision.","lang":"eng"}],"_id":"14082","acknowledgement":"The authors thank their respective lab members for feedback and helpful discussions. We thank the bioimaging and zebrafish facilities of IST Austria for their support.\r\nThis work was supported by the National Institutes of Health [R01GM112794 to A.L.M.], by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [21K06156 to T.H.], by the Grant Program for Biomedical Engineering Research from the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering [to T.H.] and by funding from the European Research Council [advanced grant 742573 to C.-P.H.]. ","year":"2023","ddc":["570"],"date_published":"2023-08-01T00:00:00Z","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"publication_status":"published","has_accepted_license":"1","intvolume":"       136","citation":{"ieee":"T. Higashi <i>et al.</i>, “ZnUMBA - a live imaging method to detect local barrier breaches,” <i>Journal of Cell Science</i>, vol. 136, no. 15. The Company of Biologists, 2023.","chicago":"Higashi, Tomohito, Rachel E. Stephenson, Cornelia Schwayer, Karla Huljev, Atsuko Y. Higashi, Carl-Philipp J Heisenberg, Hideki Chiba, and Ann L. Miller. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal of Cell Science</i>. The Company of Biologists, 2023. <a href=\"https://doi.org/10.1242/jcs.260668\">https://doi.org/10.1242/jcs.260668</a>.","short":"T. Higashi, R.E. Stephenson, C. Schwayer, K. Huljev, A.Y. Higashi, C.-P.J. Heisenberg, H. Chiba, A.L. Miller, Journal of Cell Science 136 (2023).","ama":"Higashi T, Stephenson RE, Schwayer C, et al. ZnUMBA - a live imaging method to detect local barrier breaches. <i>Journal of Cell Science</i>. 2023;136(15). doi:<a href=\"https://doi.org/10.1242/jcs.260668\">10.1242/jcs.260668</a>","mla":"Higashi, Tomohito, et al. “ZnUMBA - a Live Imaging Method to Detect Local Barrier Breaches.” <i>Journal of Cell Science</i>, vol. 136, no. 15, jcs260668, The Company of Biologists, 2023, doi:<a href=\"https://doi.org/10.1242/jcs.260668\">10.1242/jcs.260668</a>.","ista":"Higashi T, Stephenson RE, Schwayer C, Huljev K, Higashi AY, Heisenberg C-PJ, Chiba H, Miller AL. 2023. ZnUMBA - a live imaging method to detect local barrier breaches. Journal of Cell Science. 136(15), jcs260668.","apa":"Higashi, T., Stephenson, R. E., Schwayer, C., Huljev, K., Higashi, A. Y., Heisenberg, C.-P. J., … Miller, A. L. (2023). ZnUMBA - a live imaging method to detect local barrier breaches. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.260668\">https://doi.org/10.1242/jcs.260668</a>"},"status":"public","external_id":{"isi":["001070149000001"]},"article_number":"jcs260668","title":"ZnUMBA - a live imaging method to detect local barrier breaches","author":[{"full_name":"Higashi, Tomohito","first_name":"Tomohito","last_name":"Higashi"},{"full_name":"Stephenson, Rachel E.","first_name":"Rachel E.","last_name":"Stephenson"},{"last_name":"Schwayer","first_name":"Cornelia","full_name":"Schwayer, Cornelia","orcid":"0000-0001-5130-2226","id":"3436488C-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Huljev","first_name":"Karla","full_name":"Huljev, Karla","id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Higashi, Atsuko Y.","last_name":"Higashi","first_name":"Atsuko Y."},{"first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Chiba, Hideki","last_name":"Chiba","first_name":"Hideki"},{"full_name":"Miller, Ann L.","last_name":"Miller","first_name":"Ann L."}],"day":"01","file":[{"embargo_to":"open_access","creator":"dernst","embargo":"2024-08-10","content_type":"application/pdf","relation":"main_file","file_size":18665315,"file_name":"2023_JourCellScience_Higashi.pdf","access_level":"closed","date_created":"2023-08-21T07:37:54Z","checksum":"a399389b7e3d072f1788b63e612a10b3","date_updated":"2023-08-21T07:37:54Z","file_id":"14092"}],"publication":"Journal of Cell Science","article_type":"original","article_processing_charge":"No","ec_funded":1,"scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publisher":"The Company of Biologists","department":[{"_id":"CaHe"},{"_id":"EvBe"}],"doi":"10.1242/jcs.260668","quality_controlled":"1","publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"isi":1,"issue":"15","language":[{"iso":"eng"}],"project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742573"}]},{"acknowledgement":"The authors want to thank Professors Carrie Bernecky, Tom Henzinger, Martin Loose and Gaia Novarino for accepting to be interviewed, thus giving significant contribution to the discussion that lead to this article.","year":"2022","_id":"12282","abstract":[{"text":"From a simple thought to a multicellular movement","lang":"eng"}],"date_updated":"2023-08-04T10:28:04Z","type":"journal_article","oa_version":"None","month":"04","volume":135,"date_created":"2023-01-16T10:03:14Z","status":"public","external_id":{"isi":["000798123600015"],"pmid":["35438168"]},"intvolume":"       135","citation":{"mla":"Amberg, Nicole, et al. “Operation STEM Fatale – How an Equity, Diversity and Inclusion Initiative Has Brought Us to Reflect on the Current Challenges in Cell Biology and Science as a Whole.” <i>Journal of Cell Science</i>, vol. 135, no. 8, 260017, The Company of Biologists, 2022, doi:<a href=\"https://doi.org/10.1242/jcs.260017\">10.1242/jcs.260017</a>.","ista":"Amberg N, Stouffer MA, Vercellino I. 2022. Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole. Journal of Cell Science. 135(8), 260017.","apa":"Amberg, N., Stouffer, M. A., &#38; Vercellino, I. (2022). Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.260017\">https://doi.org/10.1242/jcs.260017</a>","ama":"Amberg N, Stouffer MA, Vercellino I. Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole. <i>Journal of Cell Science</i>. 2022;135(8). doi:<a href=\"https://doi.org/10.1242/jcs.260017\">10.1242/jcs.260017</a>","short":"N. Amberg, M.A. Stouffer, I. Vercellino, Journal of Cell Science 135 (2022).","ieee":"N. Amberg, M. A. Stouffer, and I. Vercellino, “Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole,” <i>Journal of Cell Science</i>, vol. 135, no. 8. The Company of Biologists, 2022.","chicago":"Amberg, Nicole, Melissa A Stouffer, and Irene Vercellino. “Operation STEM Fatale – How an Equity, Diversity and Inclusion Initiative Has Brought Us to Reflect on the Current Challenges in Cell Biology and Science as a Whole.” <i>Journal of Cell Science</i>. The Company of Biologists, 2022. <a href=\"https://doi.org/10.1242/jcs.260017\">https://doi.org/10.1242/jcs.260017</a>."},"publication_status":"published","date_published":"2022-04-19T00:00:00Z","publisher":"The Company of Biologists","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","pmid":1,"department":[{"_id":"SiHi"},{"_id":"LeSa"}],"publication":"Journal of Cell Science","article_processing_charge":"No","scopus_import":"1","article_type":"letter_note","author":[{"orcid":"0000-0002-3183-8207","id":"4CD6AAC6-F248-11E8-B48F-1D18A9856A87","full_name":"Amberg, Nicole","first_name":"Nicole","last_name":"Amberg"},{"full_name":"Stouffer, Melissa A","id":"4C9372C4-F248-11E8-B48F-1D18A9856A87","last_name":"Stouffer","first_name":"Melissa A"},{"first_name":"Irene","last_name":"Vercellino","full_name":"Vercellino, Irene","orcid":"0000-0001-5618-3449","id":"3ED6AF16-F248-11E8-B48F-1D18A9856A87"}],"day":"19","title":"Operation STEM fatale – how an equity, diversity and inclusion initiative has brought us to reflect on the current challenges in cell biology and science as a whole","article_number":"260017","isi":1,"language":[{"iso":"eng"}],"issue":"8","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"doi":"10.1242/jcs.260017","quality_controlled":"1"},{"abstract":[{"lang":"eng","text":"Neurons extend axons to form the complex circuitry of the mature brain. This depends on the coordinated response and continuous remodelling of the microtubule and F-actin networks in the axonal growth cone. Growth cone architecture remains poorly understood at nanoscales. We therefore investigated mouse hippocampal neuron growth cones using cryo-electron tomography to directly visualise their three-dimensional subcellular architecture with molecular detail. Our data showed that the hexagonal arrays of actin bundles that form filopodia penetrate and terminate deep within the growth cone interior. We directly observed the modulation of these and other growth cone actin bundles by alteration of individual F-actin helical structures. Microtubules with blunt, slightly flared or gently curved ends predominated in the growth cone, frequently contained lumenal particles and exhibited lattice defects. Investigation of the effect of absence of doublecortin, a neurodevelopmental cytoskeleton regulator, on growth cone cytoskeleton showed no major anomalies in overall growth cone organisation or in F-actin subpopulations. However, our data suggested that microtubules sustained more structural defects, highlighting the importance of microtubule integrity during growth cone migration."}],"date_updated":"2023-08-04T10:28:34Z","oa_version":"Published Version","type":"journal_article","month":"04","date_created":"2023-01-16T10:03:24Z","file_date_updated":"2023-01-30T11:41:01Z","volume":135,"year":"2022","acknowledgement":"J.A. was supported by a grant from the Medical Research Council (MRC), UK (MR/R000352/1) to C.A.M. Cryo-EM data were collected on equipment funded by the Wellcome Trust, UK (079605/Z/06/Z) and the Biotechnology and Biological Sciences Research Council (BBSRC) UK (BB/L014211/1). F.F.’s salary and institute were supported by Inserm (Institut National de la Santé et de la Recherche Médicale), CNRS (Centre National de la Recherche Scientifique) and Sorbonne Université. F.F.’s group was particularly supported by Agence Nationale de la\r\nRecherche (ANR-16-CE16-0011-03) and Seventh Framework Programme (EUHEALTH-\r\n2013, DESIRE, N° 60253; also funding M.S.’s salary) and the European Cooperation in Science and Technology (COST Action CA16118). Open Access funding provided by Birkbeck College: Birkbeck University of London. Deposited in PMC for immediate release.","_id":"12283","has_accepted_license":"1","publication_status":"published","oa":1,"ddc":["570"],"date_published":"2022-04-01T00:00:00Z","external_id":{"pmid":["35383828"],"isi":["000783840400010"]},"status":"public","citation":{"ieee":"J. Atherton, M. A. Stouffer, F. Francis, and C. A. Moores, “Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography,” <i>Journal of Cell Science</i>, vol. 135, no. 7. The Company of Biologists, 2022.","chicago":"Atherton, Joseph, Melissa A Stouffer, Fiona Francis, and Carolyn A. Moores. “Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron Tomography.” <i>Journal of Cell Science</i>. The Company of Biologists, 2022. <a href=\"https://doi.org/10.1242/jcs.259234\">https://doi.org/10.1242/jcs.259234</a>.","short":"J. Atherton, M.A. Stouffer, F. Francis, C.A. Moores, Journal of Cell Science 135 (2022).","ama":"Atherton J, Stouffer MA, Francis F, Moores CA. Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. <i>Journal of Cell Science</i>. 2022;135(7). doi:<a href=\"https://doi.org/10.1242/jcs.259234\">10.1242/jcs.259234</a>","mla":"Atherton, Joseph, et al. “Visualising the Cytoskeletal Machinery in Neuronal Growth Cones Using Cryo-Electron Tomography.” <i>Journal of Cell Science</i>, vol. 135, no. 7, 259234, The Company of Biologists, 2022, doi:<a href=\"https://doi.org/10.1242/jcs.259234\">10.1242/jcs.259234</a>.","ista":"Atherton J, Stouffer MA, Francis F, Moores CA. 2022. Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. Journal of Cell Science. 135(7), 259234.","apa":"Atherton, J., Stouffer, M. A., Francis, F., &#38; Moores, C. A. (2022). Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.259234\">https://doi.org/10.1242/jcs.259234</a>"},"intvolume":"       135","file":[{"creator":"dernst","content_type":"application/pdf","relation":"main_file","file_size":13868733,"success":1,"file_name":"2022_JourCellBiology_Atherton.pdf","access_level":"open_access","date_created":"2023-01-30T11:41:01Z","checksum":"4346ed32cb7c89a8ca051c7da68a9a1c","date_updated":"2023-01-30T11:41:01Z","file_id":"12461"}],"day":"01","author":[{"first_name":"Joseph","last_name":"Atherton","full_name":"Atherton, Joseph"},{"first_name":"Melissa A","last_name":"Stouffer","full_name":"Stouffer, Melissa A","id":"4C9372C4-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Fiona","last_name":"Francis","full_name":"Francis, Fiona"},{"full_name":"Moores, Carolyn A.","first_name":"Carolyn A.","last_name":"Moores"}],"title":"Visualising the cytoskeletal machinery in neuronal growth cones using cryo-electron tomography","article_number":"259234","pmid":1,"department":[{"_id":"SiHi"}],"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publisher":"The Company of Biologists","scopus_import":"1","article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)"},"article_type":"original","publication":"Journal of Cell Science","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"quality_controlled":"1","doi":"10.1242/jcs.259234","language":[{"iso":"eng"}],"issue":"7","isi":1,"keyword":["Cell Biology"]},{"oa":1,"publication_status":"published","has_accepted_license":"1","date_published":"2020-08-06T00:00:00Z","ddc":["575"],"acknowledged_ssus":[{"_id":"EM-Fac"},{"_id":"Bio"}],"status":"public","external_id":{"pmid":["32616560"],"isi":["000561047900021"]},"intvolume":"       133","related_material":{"record":[{"relation":"dissertation_contains","id":"14510","status":"public"}]},"citation":{"chicago":"Johnson, Alexander J, Nataliia Gnyliukh, Walter Kaufmann, Madhumitha Narasimhan, G Vert, SY Bednarek, and Jiří Friml. “Experimental Toolbox for Quantitative Evaluation of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” <i>Journal of Cell Science</i>. The Company of Biologists, 2020. <a href=\"https://doi.org/10.1242/jcs.248062\">https://doi.org/10.1242/jcs.248062</a>.","ieee":"A. J. Johnson <i>et al.</i>, “Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis,” <i>Journal of Cell Science</i>, vol. 133, no. 15. The Company of Biologists, 2020.","short":"A.J. Johnson, N. Gnyliukh, W. Kaufmann, M. Narasimhan, G. Vert, S. Bednarek, J. Friml, Journal of Cell Science 133 (2020).","ama":"Johnson AJ, Gnyliukh N, Kaufmann W, et al. Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. <i>Journal of Cell Science</i>. 2020;133(15). doi:<a href=\"https://doi.org/10.1242/jcs.248062\">10.1242/jcs.248062</a>","apa":"Johnson, A. J., Gnyliukh, N., Kaufmann, W., Narasimhan, M., Vert, G., Bednarek, S., &#38; Friml, J. (2020). Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.248062\">https://doi.org/10.1242/jcs.248062</a>","mla":"Johnson, Alexander J., et al. “Experimental Toolbox for Quantitative Evaluation of Clathrin-Mediated Endocytosis in the Plant Model Arabidopsis.” <i>Journal of Cell Science</i>, vol. 133, no. 15, jcs248062, The Company of Biologists, 2020, doi:<a href=\"https://doi.org/10.1242/jcs.248062\">10.1242/jcs.248062</a>.","ista":"Johnson AJ, Gnyliukh N, Kaufmann W, Narasimhan M, Vert G, Bednarek S, Friml J. 2020. Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis. Journal of Cell Science. 133(15), jcs248062."},"abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) is a crucial cellular process implicated in many aspects of plant growth, development, intra- and inter-cellular signaling, nutrient uptake and pathogen defense. Despite these significant roles, little is known about the precise molecular details of how it functions in planta. In order to facilitate the direct quantitative study of plant CME, here we review current routinely used methods and present refined, standardized quantitative imaging protocols which allow the detailed characterization of CME at multiple scales in plant tissues. These include: (i) an efficient electron microscopy protocol for the imaging of Arabidopsis CME vesicles in situ, thus providing a method for the detailed characterization of the ultra-structure of clathrin-coated vesicles; (ii) a detailed protocol and analysis for quantitative live-cell fluorescence microscopy to precisely examine the temporal interplay of endocytosis components during single CME events; (iii) a semi-automated analysis to allow the quantitative characterization of global internalization of cargos in whole plant tissues; and (iv) an overview and validation of useful genetic and pharmacological tools to interrogate the molecular mechanisms and function of CME in intact plant samples."}],"date_updated":"2023-12-01T13:51:07Z","oa_version":"Published Version","type":"journal_article","month":"08","volume":133,"date_created":"2020-07-21T08:58:19Z","file_date_updated":"2021-08-08T22:30:03Z","acknowledgement":"This paper is dedicated to the memory of Christien Merrifield. He pioneered quantitative\r\nimaging approaches in mammalian CME and his mentorship inspired the development of all\r\nthe analysis methods presented here. His joy in research, pure scientific curiosity and\r\nmicroscopy excellence remain a constant inspiration. We thank Daniel Van Damme for gifting\r\nus the CLC2-GFP x TPLATE-TagRFP plants used in this manuscript. We further thank the\r\nScientific Service Units at IST Austria; specifically, the Electron Microscopy Facility for\r\ntechnical assistance (in particular Vanessa Zheden) and the BioImaging Facility BioImaging\r\nFacility for access to equipment. ","year":"2020","_id":"8139","publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"doi":"10.1242/jcs.248062","quality_controlled":"1","project":[{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630"},{"name":"International IST Doctoral Program","call_identifier":"H2020","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385"}],"isi":1,"language":[{"iso":"eng"}],"issue":"15","author":[{"full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson"},{"last_name":"Gnyliukh","first_name":"Nataliia","full_name":"Gnyliukh, Nataliia","id":"390C1120-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2198-0509"},{"first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Narasimhan, Madhumitha","orcid":"0000-0002-8600-0671","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","last_name":"Narasimhan","first_name":"Madhumitha"},{"last_name":"Vert","first_name":"G","full_name":"Vert, G"},{"full_name":"Bednarek, SY","first_name":"SY","last_name":"Bednarek"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"}],"file":[{"content_type":"application/pdf","relation":"main_file","embargo":"2021-08-07","file_size":15150403,"creator":"ajohnson","file_name":"2020 - Johnson - JSC - plant CME toolbox.pdf","date_created":"2020-11-26T17:12:51Z","access_level":"open_access","date_updated":"2021-08-08T22:30:03Z","file_id":"8815","checksum":"2d11f79a0b4e0a380fb002b933da331a"}],"day":"06","title":"Experimental toolbox for quantitative evaluation of clathrin-mediated endocytosis in the plant model Arabidopsis","article_number":"jcs248062","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"The Company of Biologists","pmid":1,"department":[{"_id":"JiFr"},{"_id":"EM-Fac"}],"publication":"Journal of Cell Science","article_processing_charge":"No","scopus_import":"1","ec_funded":1,"article_type":"original"},{"publisher":"The Company of Biologists","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","department":[{"_id":"FlSc"}],"pmid":1,"publication":"Journal of Cell Science","article_type":"original","article_processing_charge":"No","author":[{"first_name":"Georgi A","last_name":"Dimchev","full_name":"Dimchev, Georgi A","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161"},{"full_name":"Amiri, Behnam","first_name":"Behnam","last_name":"Amiri"},{"first_name":"Ashley C.","last_name":"Humphries","full_name":"Humphries, Ashley C."},{"full_name":"Schaks, Matthias","last_name":"Schaks","first_name":"Matthias"},{"full_name":"Dimchev, Vanessa","first_name":"Vanessa","last_name":"Dimchev"},{"last_name":"Stradal","first_name":"Theresia E. B.","full_name":"Stradal, Theresia E. B."},{"last_name":"Faix","first_name":"Jan","full_name":"Faix, Jan"},{"first_name":"Matthias","last_name":"Krause","full_name":"Krause, Matthias"},{"first_name":"Michael","last_name":"Way","full_name":"Way, Michael"},{"last_name":"Falcke","first_name":"Martin","full_name":"Falcke, Martin"},{"full_name":"Rottner, Klemens","first_name":"Klemens","last_name":"Rottner"}],"file":[{"checksum":"ba917e551acc4ece2884b751434df9ae","date_updated":"2020-10-11T22:30:02Z","file_id":"8435","access_level":"open_access","date_created":"2020-09-17T14:07:51Z","file_name":"2020_JournalCellScience_Dimchev.pdf","creator":"dernst","content_type":"application/pdf","relation":"main_file","embargo":"2020-10-10","file_size":13493302}],"day":"09","article_number":"jcs239020","title":"Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation","project":[{"_id":"2674F658-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Protein structure and function in filopodia across scales","grant_number":"M02495"}],"keyword":["Cell Biology"],"isi":1,"issue":"7","language":[{"iso":"eng"}],"publication_identifier":{"eissn":["1477-9137"],"issn":["0021-9533"]},"doi":"10.1242/jcs.239020","quality_controlled":"1","acknowledgement":"This work was supported in part by Deutsche Forschungsgemeinschaft (DFG)[GRK2223/1, RO2414/5-1 (to K.R.), FA350/11-1 (to M.F.) and FA330/11-1 (to J.F.)],as well as by intramural funding from the Helmholtz Association (to T.E.B.S. andK.R.). G.D. was additionally funded by the Austrian Science Fund (FWF) LiseMeitner Program [M-2495]. A.C.H. and M.W. are supported by the Francis CrickInstitute, which receives its core funding from Cancer Research UK [FC001209], theMedical Research Council [FC001209] and the Wellcome Trust [FC001209]. M.K. issupported by the Biotechnology and Biological Sciences Research Council [BB/F011431/1, BB/J000590/1, BB/N000226/1]. Deposited in PMC for release after 6months.","year":"2020","_id":"8434","type":"journal_article","month":"04","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Efficient migration on adhesive surfaces involves the protrusion of lamellipodial actin networks and their subsequent stabilization by nascent adhesions. The actin-binding protein lamellipodin (Lpd) is thought to play a critical role in lamellipodium protrusion, by delivering Ena/VASP proteins onto the growing plus ends of actin filaments and by interacting with the WAVE regulatory complex, an activator of the Arp2/3 complex, at the leading edge. Using B16-F1 melanoma cell lines, we demonstrate that genetic ablation of Lpd compromises protrusion efficiency and coincident cell migration without altering essential parameters of lamellipodia, including their maximal rate of forward advancement and actin polymerization. We also confirmed lamellipodia and migration phenotypes with CRISPR/Cas9-mediated Lpd knockout Rat2 fibroblasts, excluding cell type-specific effects. Moreover, computer-aided analysis of cell-edge morphodynamics on B16-F1 cell lamellipodia revealed that loss of Lpd correlates with reduced temporal protrusion maintenance as a prerequisite of nascent adhesion formation. We conclude that Lpd optimizes protrusion and nascent adhesion formation by counteracting frequent, chaotic retraction and membrane ruffling.This article has an associated First Person interview with the first author of the paper. "}],"date_updated":"2023-09-05T15:41:48Z","volume":133,"file_date_updated":"2020-10-11T22:30:02Z","date_created":"2020-09-17T14:00:33Z","external_id":{"pmid":[" 32094266"],"isi":["000534387800005"]},"status":"public","intvolume":"       133","citation":{"ieee":"G. A. Dimchev <i>et al.</i>, “Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation,” <i>Journal of Cell Science</i>, vol. 133, no. 7. The Company of Biologists, 2020.","chicago":"Dimchev, Georgi A, Behnam Amiri, Ashley C. Humphries, Matthias Schaks, Vanessa Dimchev, Theresia E. B. Stradal, Jan Faix, et al. “Lamellipodin Tunes Cell Migration by Stabilizing Protrusions and Promoting Adhesion Formation.” <i>Journal of Cell Science</i>. The Company of Biologists, 2020. <a href=\"https://doi.org/10.1242/jcs.239020\">https://doi.org/10.1242/jcs.239020</a>.","short":"G.A. Dimchev, B. Amiri, A.C. Humphries, M. Schaks, V. Dimchev, T.E.B. Stradal, J. Faix, M. Krause, M. Way, M. Falcke, K. Rottner, Journal of Cell Science 133 (2020).","ama":"Dimchev GA, Amiri B, Humphries AC, et al. Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. <i>Journal of Cell Science</i>. 2020;133(7). doi:<a href=\"https://doi.org/10.1242/jcs.239020\">10.1242/jcs.239020</a>","mla":"Dimchev, Georgi A., et al. “Lamellipodin Tunes Cell Migration by Stabilizing Protrusions and Promoting Adhesion Formation.” <i>Journal of Cell Science</i>, vol. 133, no. 7, jcs239020, The Company of Biologists, 2020, doi:<a href=\"https://doi.org/10.1242/jcs.239020\">10.1242/jcs.239020</a>.","ista":"Dimchev GA, Amiri B, Humphries AC, Schaks M, Dimchev V, Stradal TEB, Faix J, Krause M, Way M, Falcke M, Rottner K. 2020. Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. Journal of Cell Science. 133(7), jcs239020.","apa":"Dimchev, G. A., Amiri, B., Humphries, A. C., Schaks, M., Dimchev, V., Stradal, T. E. B., … Rottner, K. (2020). Lamellipodin tunes cell migration by stabilizing protrusions and promoting adhesion formation. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.239020\">https://doi.org/10.1242/jcs.239020</a>"},"oa":1,"publication_status":"published","has_accepted_license":"1","date_published":"2020-04-09T00:00:00Z","ddc":["570"]},{"year":"2019","_id":"7420","type":"journal_article","month":"06","oa_version":"Published Version","date_updated":"2023-09-06T15:01:00Z","abstract":[{"text":"β1-integrins mediate cell–matrix interactions and their trafficking is important in the dynamic regulation of cell adhesion, migration and malignant processes, including cancer cell invasion. Here, we employ an RNAi screen to characterize regulators of integrin traffic and identify the association of Golgi-localized gamma ear-containing Arf-binding protein 2 (GGA2) with β1-integrin, and its role in recycling of active but not inactive β1-integrin receptors. Silencing of GGA2 limits active β1-integrin levels in focal adhesions and decreases cancer cell migration and invasion, which is in agreement with its ability to regulate the dynamics of active integrins. By using the proximity-dependent biotin identification (BioID) method, we identified two RAB family small GTPases, i.e. RAB13 and RAB10, as novel interactors of GGA2. Functionally, RAB13 silencing triggers the intracellular accumulation of active β1-integrin, and reduces integrin activity in focal adhesions and cell migration similarly to GGA2 depletion, indicating that both facilitate active β1-integrin recycling to the plasma membrane. Thus, GGA2 and RAB13 are important specificity determinants for integrin activity-dependent traffic.","lang":"eng"}],"date_created":"2020-01-30T10:31:42Z","volume":132,"external_id":{"pmid":["31076515"],"isi":["000473327900017"]},"status":"public","citation":{"ama":"Sahgal P, Alanko JH, Icha J, et al. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. <i>Journal of Cell Science</i>. 2019;132(11). doi:<a href=\"https://doi.org/10.1242/jcs.233387\">10.1242/jcs.233387</a>","apa":"Sahgal, P., Alanko, J. H., Icha, J., Paatero, I., Hamidi, H., Arjonen, A., … Ivaska, J. (2019). GGA2 and RAB13 promote activity-dependent β1-integrin recycling. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.233387\">https://doi.org/10.1242/jcs.233387</a>","ista":"Sahgal P, Alanko JH, Icha J, Paatero I, Hamidi H, Arjonen A, Pietilä M, Rokka A, Ivaska J. 2019. GGA2 and RAB13 promote activity-dependent β1-integrin recycling. Journal of Cell Science. 132(11), jcs233387.","mla":"Sahgal, Pranshu, et al. “GGA2 and RAB13 Promote Activity-Dependent Β1-Integrin Recycling.” <i>Journal of Cell Science</i>, vol. 132, no. 11, jcs233387, The Company of Biologists, 2019, doi:<a href=\"https://doi.org/10.1242/jcs.233387\">10.1242/jcs.233387</a>.","chicago":"Sahgal, Pranshu, Jonna H Alanko, Jaroslav Icha, Ilkka Paatero, Hellyeh Hamidi, Antti Arjonen, Mika Pietilä, Anne Rokka, and Johanna Ivaska. “GGA2 and RAB13 Promote Activity-Dependent Β1-Integrin Recycling.” <i>Journal of Cell Science</i>. The Company of Biologists, 2019. <a href=\"https://doi.org/10.1242/jcs.233387\">https://doi.org/10.1242/jcs.233387</a>.","ieee":"P. Sahgal <i>et al.</i>, “GGA2 and RAB13 promote activity-dependent β1-integrin recycling,” <i>Journal of Cell Science</i>, vol. 132, no. 11. The Company of Biologists, 2019.","short":"P. Sahgal, J.H. Alanko, J. Icha, I. Paatero, H. Hamidi, A. Arjonen, M. Pietilä, A. Rokka, J. Ivaska, Journal of Cell Science 132 (2019)."},"intvolume":"       132","oa":1,"publication_status":"published","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/jcs.233387"}],"date_published":"2019-06-07T00:00:00Z","department":[{"_id":"MiSi"}],"pmid":1,"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"The Company of Biologists","article_type":"original","article_processing_charge":"No","publication":"Journal of Cell Science","day":"07","author":[{"last_name":"Sahgal","first_name":"Pranshu","full_name":"Sahgal, Pranshu"},{"id":"2CC12E8C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7698-3061","full_name":"Alanko, Jonna H","last_name":"Alanko","first_name":"Jonna H"},{"first_name":"Jaroslav","last_name":"Icha","full_name":"Icha, Jaroslav"},{"full_name":"Paatero, Ilkka","first_name":"Ilkka","last_name":"Paatero"},{"first_name":"Hellyeh","last_name":"Hamidi","full_name":"Hamidi, Hellyeh"},{"last_name":"Arjonen","first_name":"Antti","full_name":"Arjonen, Antti"},{"full_name":"Pietilä, Mika","first_name":"Mika","last_name":"Pietilä"},{"full_name":"Rokka, Anne","first_name":"Anne","last_name":"Rokka"},{"first_name":"Johanna","last_name":"Ivaska","full_name":"Ivaska, Johanna"}],"article_number":"jcs233387","title":"GGA2 and RAB13 promote activity-dependent β1-integrin recycling","issue":"11","language":[{"iso":"eng"}],"isi":1,"publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"quality_controlled":"1","doi":"10.1242/jcs.233387"},{"language":[{"iso":"eng"}],"issue":"2","keyword":["Cell Biology"],"publication_identifier":{"issn":["0021-9533"],"eissn":["1477-9137"]},"quality_controlled":"1","doi":"10.1242/jcs.005777","pmid":1,"user_id":"72615eeb-f1f3-11ec-aa25-d4573ddc34fd","publisher":"The Company of Biologists","article_processing_charge":"No","scopus_import":"1","article_type":"letter_note","publication":"Journal of Cell Science","day":"15","author":[{"first_name":"Daniel J.","last_name":"Anderson","full_name":"Anderson, Daniel J."},{"full_name":"HETZER, Martin W","orcid":"0000-0002-2111-992X","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","first_name":"Martin W","last_name":"HETZER"}],"title":"Shaping the endoplasmic reticulum into the nuclear envelope","external_id":{"pmid":["18187447"]},"status":"public","citation":{"ama":"Anderson DJ, Hetzer M. Shaping the endoplasmic reticulum into the nuclear envelope. <i>Journal of Cell Science</i>. 2008;121(2):137-142. doi:<a href=\"https://doi.org/10.1242/jcs.005777\">10.1242/jcs.005777</a>","apa":"Anderson, D. J., &#38; Hetzer, M. (2008). Shaping the endoplasmic reticulum into the nuclear envelope. <i>Journal of Cell Science</i>. The Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.005777\">https://doi.org/10.1242/jcs.005777</a>","mla":"Anderson, Daniel J., and Martin Hetzer. “Shaping the Endoplasmic Reticulum into the Nuclear Envelope.” <i>Journal of Cell Science</i>, vol. 121, no. 2, The Company of Biologists, 2008, pp. 137–42, doi:<a href=\"https://doi.org/10.1242/jcs.005777\">10.1242/jcs.005777</a>.","ista":"Anderson DJ, Hetzer M. 2008. Shaping the endoplasmic reticulum into the nuclear envelope. Journal of Cell Science. 121(2), 137–142.","chicago":"Anderson, Daniel J., and Martin Hetzer. “Shaping the Endoplasmic Reticulum into the Nuclear Envelope.” <i>Journal of Cell Science</i>. The Company of Biologists, 2008. <a href=\"https://doi.org/10.1242/jcs.005777\">https://doi.org/10.1242/jcs.005777</a>.","ieee":"D. J. Anderson and M. Hetzer, “Shaping the endoplasmic reticulum into the nuclear envelope,” <i>Journal of Cell Science</i>, vol. 121, no. 2. The Company of Biologists, pp. 137–142, 2008.","short":"D.J. Anderson, M. Hetzer, Journal of Cell Science 121 (2008) 137–142."},"intvolume":"       121","extern":"1","publication_status":"published","oa":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1242/jcs.005777"}],"date_published":"2008-01-15T00:00:00Z","year":"2008","_id":"11113","abstract":[{"text":"The nuclear envelope (NE), a double membrane enclosing the nucleus of eukaryotic cells, controls the flow of information between the nucleoplasm and the cytoplasm and provides a scaffold for the organization of chromatin and the cytoskeleton. In dividing metazoan cells, the NE breaks down at the onset of mitosis and then reforms around segregated chromosomes to generate the daughter nuclei. Recent data from intact cells and cell-free nuclear assembly systems suggest that the endoplasmic reticulum (ER) is the source of membrane for NE assembly. At the end of mitosis, ER membrane tubules are targeted to chromatin via tubule ends and reorganized into flat nuclear membrane sheets by specific DNA-binding membrane proteins. In contrast to previous models, which proposed vesicle fusion to be the principal mechanism of NE formation, these new studies suggest that the nuclear membrane forms by the chromatin-mediated reshaping of the ER.","lang":"eng"}],"date_updated":"2022-07-18T08:56:10Z","month":"01","oa_version":"Published Version","type":"journal_article","page":"137-142","date_created":"2022-04-07T07:55:46Z","volume":121}]