---
_id: '7234'
abstract:
- lang: eng
text: T lymphocytes utilize amoeboid migration to navigate effectively within complex
microenvironments. The precise rearrangement of the actin cytoskeleton required
for cellular forward propulsion is mediated by actin regulators, including the
actin‐related protein 2/3 (Arp2/3) complex, a macromolecular machine that nucleates
branched actin filaments at the leading edge. The consequences of modulating Arp2/3
activity on the biophysical properties of the actomyosin cortex and downstream
T cell function are incompletely understood. We report that even a moderate decrease
of Arp3 levels in T cells profoundly affects actin cortex integrity. Reduction
in total F‐actin content leads to reduced cortical tension and disrupted lamellipodia
formation. Instead, in Arp3‐knockdown cells, the motility mode is dominated by
blebbing migration characterized by transient, balloon‐like protrusions at the
leading edge. Although this migration mode seems to be compatible with interstitial
migration in three‐dimensional environments, diminished locomotion kinetics and
impaired cytotoxicity interfere with optimal T cell function. These findings define
the importance of finely tuned, Arp2/3‐dependent mechanophysical membrane integrity
in cytotoxic effector T lymphocyte activities.
article_processing_charge: No
article_type: original
author:
- first_name: Peyman
full_name: Obeidy, Peyman
last_name: Obeidy
- first_name: Lining A.
full_name: Ju, Lining A.
last_name: Ju
- first_name: Stefan H.
full_name: Oehlers, Stefan H.
last_name: Oehlers
- first_name: Nursafwana S.
full_name: Zulkhernain, Nursafwana S.
last_name: Zulkhernain
- first_name: Quintin
full_name: Lee, Quintin
last_name: Lee
- first_name: Jorge L.
full_name: Galeano Niño, Jorge L.
last_name: Galeano Niño
- first_name: Rain Y.Q.
full_name: Kwan, Rain Y.Q.
last_name: Kwan
- first_name: Shweta
full_name: Tikoo, Shweta
last_name: Tikoo
- first_name: Lois L.
full_name: Cavanagh, Lois L.
last_name: Cavanagh
- first_name: Paulus
full_name: Mrass, Paulus
last_name: Mrass
- first_name: Adam J.L.
full_name: Cook, Adam J.L.
last_name: Cook
- first_name: Shaun P.
full_name: Jackson, Shaun P.
last_name: Jackson
- first_name: Maté
full_name: Biro, Maté
last_name: Biro
- first_name: Ben
full_name: Roediger, Ben
last_name: Roediger
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Wolfgang
full_name: Weninger, Wolfgang
last_name: Weninger
citation:
ama: Obeidy P, Ju LA, Oehlers SH, et al. Partial loss of actin nucleator actin-related
protein 2/3 activity triggers blebbing in primary T lymphocytes. Immunology
and Cell Biology. 2020;98(2):93-113. doi:10.1111/imcb.12304
apa: Obeidy, P., Ju, L. A., Oehlers, S. H., Zulkhernain, N. S., Lee, Q., Galeano
Niño, J. L., … Weninger, W. (2020). Partial loss of actin nucleator actin-related
protein 2/3 activity triggers blebbing in primary T lymphocytes. Immunology
and Cell Biology. Wiley. https://doi.org/10.1111/imcb.12304
chicago: Obeidy, Peyman, Lining A. Ju, Stefan H. Oehlers, Nursafwana S. Zulkhernain,
Quintin Lee, Jorge L. Galeano Niño, Rain Y.Q. Kwan, et al. “Partial Loss of Actin
Nucleator Actin-Related Protein 2/3 Activity Triggers Blebbing in Primary T Lymphocytes.”
Immunology and Cell Biology. Wiley, 2020. https://doi.org/10.1111/imcb.12304.
ieee: P. Obeidy et al., “Partial loss of actin nucleator actin-related protein
2/3 activity triggers blebbing in primary T lymphocytes,” Immunology and Cell
Biology, vol. 98, no. 2. Wiley, pp. 93–113, 2020.
ista: Obeidy P, Ju LA, Oehlers SH, Zulkhernain NS, Lee Q, Galeano Niño JL, Kwan
RYQ, Tikoo S, Cavanagh LL, Mrass P, Cook AJL, Jackson SP, Biro M, Roediger B,
Sixt MK, Weninger W. 2020. Partial loss of actin nucleator actin-related protein
2/3 activity triggers blebbing in primary T lymphocytes. Immunology and Cell Biology.
98(2), 93–113.
mla: Obeidy, Peyman, et al. “Partial Loss of Actin Nucleator Actin-Related Protein
2/3 Activity Triggers Blebbing in Primary T Lymphocytes.” Immunology and Cell
Biology, vol. 98, no. 2, Wiley, 2020, pp. 93–113, doi:10.1111/imcb.12304.
short: P. Obeidy, L.A. Ju, S.H. Oehlers, N.S. Zulkhernain, Q. Lee, J.L. Galeano
Niño, R.Y.Q. Kwan, S. Tikoo, L.L. Cavanagh, P. Mrass, A.J.L. Cook, S.P. Jackson,
M. Biro, B. Roediger, M.K. Sixt, W. Weninger, Immunology and Cell Biology 98 (2020)
93–113.
date_created: 2020-01-05T23:00:48Z
date_published: 2020-02-01T00:00:00Z
date_updated: 2023-08-17T14:21:12Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1111/imcb.12304
external_id:
isi:
- '000503885600001'
pmid:
- '31698518'
file:
- access_level: open_access
checksum: c389477b4b52172ef76afff8a06c6775
content_type: application/pdf
creator: dernst
date_created: 2020-11-19T11:22:33Z
date_updated: 2020-11-19T11:22:33Z
file_id: '8775'
file_name: 2020_ImmunologyCellBio_Obeidy.pdf
file_size: 8569945
relation: main_file
success: 1
file_date_updated: 2020-11-19T11:22:33Z
has_accepted_license: '1'
intvolume: ' 98'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 93-113
pmid: 1
publication: Immunology and Cell Biology
publication_identifier:
eissn:
- '14401711'
issn:
- '08189641'
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Partial loss of actin nucleator actin-related protein 2/3 activity triggers
blebbing in primary T lymphocytes
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 98
year: '2020'
...