---
_id: '2528'
abstract:
- lang: eng
  text: We previously reported a novel rat membrane protein that exhibits a voltage-dependent
    potassium channel activity on the basis of molecular cloning combined with an
    electrophysiological assay. This protein, termed I(sK) protein, is small and different
    from the conventional potassium channel proteins but induces selective permeation
    of potassium ions on its expression in Xenopus oocytes. In this investigation,
    we examined cellular localization of rat I(sK) protein by preparing three different
    types of antibody that specifically reacts with a distinct part of rat I(sK) protein.
    Immunohistochemical analysis using these antibody preparations demonstrated that
    rat I(sK) protein is confined to the apical membrane portion of epithelial cells
    in the proximal tubule of the kidney, the submandibular duct and the uterine endometrium.
    The observed tissue distribution of rat I(sK) protein was consistent with that
    of the I(sK) protein mRNA determined by blot hybridization analysis. In epithelial
    cells, the sodium, potassium-ATPase pump in the basolateral membrane generates
    a sodium gradient across the epithelial cell and allows sodium ions to enter the
    cell through the apical membrane. Thus, taking into account the cellular localization
    of the I(sK) protein, together with its electrophysiological properties, we discussed
    a possible function of the I(sK) protein, namely that this protein is involved
    in potassium permeation in the apical membrane of epithelial cells through the
    depolarizing effect of sodium entry.
article_processing_charge: No
article_type: original
author:
- first_name: Tetsuo
  full_name: Sugimoto, Tetsuo
  last_name: Sugimoto
- first_name: Yasuto
  full_name: Tanabe, Yasuto
  last_name: Tanabe
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Masazumi
  full_name: Iwai, Masazumi
  last_name: Iwai
- first_name: Toru
  full_name: Takumi, Toru
  last_name: Takumi
- first_name: Hiroaki
  full_name: Ohkubo, Hiroaki
  last_name: Ohkubo
- first_name: Shigetada
  full_name: Nakanishi, Shigetada
  last_name: Nakanishi
citation:
  ama: 'Sugimoto T, Tanabe Y, Shigemoto R, et al. Immunohistochemical study of a rat
    membrane protein which induces a selective potassium permeation: Its localization
    in the apical membrane portion of epithelial cells. <i>Journal of Membrane Biology</i>.
    1990;113(1):39-47. doi:<a href="https://doi.org/10.1007/BF01869604">10.1007/BF01869604</a>'
  apa: 'Sugimoto, T., Tanabe, Y., Shigemoto, R., Iwai, M., Takumi, T., Ohkubo, H.,
    &#38; Nakanishi, S. (1990). Immunohistochemical study of a rat membrane protein
    which induces a selective potassium permeation: Its localization in the apical
    membrane portion of epithelial cells. <i>Journal of Membrane Biology</i>. Springer.
    <a href="https://doi.org/10.1007/BF01869604">https://doi.org/10.1007/BF01869604</a>'
  chicago: 'Sugimoto, Tetsuo, Yasuto Tanabe, Ryuichi Shigemoto, Masazumi Iwai, Toru
    Takumi, Hiroaki Ohkubo, and Shigetada Nakanishi. “Immunohistochemical Study of
    a Rat Membrane Protein Which Induces a Selective Potassium Permeation: Its Localization
    in the Apical Membrane Portion of Epithelial Cells.” <i>Journal of Membrane Biology</i>.
    Springer, 1990. <a href="https://doi.org/10.1007/BF01869604">https://doi.org/10.1007/BF01869604</a>.'
  ieee: 'T. Sugimoto <i>et al.</i>, “Immunohistochemical study of a rat membrane protein
    which induces a selective potassium permeation: Its localization in the apical
    membrane portion of epithelial cells,” <i>Journal of Membrane Biology</i>, vol.
    113, no. 1. Springer, pp. 39–47, 1990.'
  ista: 'Sugimoto T, Tanabe Y, Shigemoto R, Iwai M, Takumi T, Ohkubo H, Nakanishi
    S. 1990. Immunohistochemical study of a rat membrane protein which induces a selective
    potassium permeation: Its localization in the apical membrane portion of epithelial
    cells. Journal of Membrane Biology. 113(1), 39–47.'
  mla: 'Sugimoto, Tetsuo, et al. “Immunohistochemical Study of a Rat Membrane Protein
    Which Induces a Selective Potassium Permeation: Its Localization in the Apical
    Membrane Portion of Epithelial Cells.” <i>Journal of Membrane Biology</i>, vol.
    113, no. 1, Springer, 1990, pp. 39–47, doi:<a href="https://doi.org/10.1007/BF01869604">10.1007/BF01869604</a>.'
  short: T. Sugimoto, Y. Tanabe, R. Shigemoto, M. Iwai, T. Takumi, H. Ohkubo, S. Nakanishi,
    Journal of Membrane Biology 113 (1990) 39–47.
date_created: 2018-12-11T11:58:12Z
date_published: 1990-01-01T00:00:00Z
date_updated: 2022-02-24T09:55:39Z
day: '01'
doi: 10.1007/BF01869604
extern: '1'
external_id:
  pmid:
  - '2154581'
intvolume: '       113'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://link.springer.com/article/10.1007/BF01869604
month: '01'
oa_version: None
page: 39 - 47
pmid: 1
publication: Journal of Membrane Biology
publication_identifier:
  eissn:
  - 1432-1424
  issn:
  - 0022-2631
publication_status: published
publisher: Springer
publist_id: '4371'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Immunohistochemical study of a rat membrane protein which induces a selective
  potassium permeation: Its localization in the apical membrane portion of epithelial
  cells'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 113
year: '1990'
...
---
_id: '3465'
abstract:
- lang: eng
  text: Asymmetrical displacement currents and Na currents of single myelinated nerve
    fibers of Xenopus laevis were studied in the temperature range from 5 to 24 degrees
    C. The time constant of the on-response at E = 4 mV, tau on, was strongly temperature
    dependent, whereas the amount of displaced charge at E = 39 mV, Qon, was only
    slightly temperature dependent. The mean Q10 for tau on-1 was 2.54, the mean Q10
    for Qon was 1.07. The time constant of charge immobilization, tau i, at E = 4
    mV varied significantly (alpha = 0.001) with temperature. The mean Q10 for tau
    i-1 was 2.71 +/- 0.38. The time constants of immobilization of gating charge and
    of fast inactivation of Na permeability were similar in the temperature range
    from 6 to 22 degrees C. The Qoff/Qon ratio for E = 4 mV pulses of 0.5 msec duration
    decreased with increasing temperature. The temperature dependence of the time
    constant of the off-response could not be described by a single Q10 value, since
    the Q10 depended on the duration of the test pulse. Increasing temperature shifted
    Qon (E) curves to more negative potentials by 0.51 mV K-1, but shifted PNa (E)
    curves and h infinity (E) curves to more positive potentials by 0.43 and 0.57
    mV K-1, respectively. h infinity (E = -70 mV) increased monotonously with increasing
    temperature. The present data indicate that considerable entropy changes may occur
    when the Na channel molecule passes from closed through open to inactivated states.
article_processing_charge: No
article_type: original
author:
- first_name: Peter M
  full_name: Jonas, Peter M
  id: 353C1B58-F248-11E8-B48F-1D18A9856A87
  last_name: Jonas
  orcid: 0000-0001-5001-4804
citation:
  ama: Jonas PM. Temperature dependence of gating current in myelinated nerve fibers.
    <i>Journal of Membrane Biology</i>. 1989;112(3):277-289. doi:<a href="https://doi.org/10.1007/BF01870958">10.1007/BF01870958</a>
  apa: Jonas, P. M. (1989). Temperature dependence of gating current in myelinated
    nerve fibers. <i>Journal of Membrane Biology</i>. Springer. <a href="https://doi.org/10.1007/BF01870958">https://doi.org/10.1007/BF01870958</a>
  chicago: Jonas, Peter M. “Temperature Dependence of Gating Current in Myelinated
    Nerve Fibers.” <i>Journal of Membrane Biology</i>. Springer, 1989. <a href="https://doi.org/10.1007/BF01870958">https://doi.org/10.1007/BF01870958</a>.
  ieee: P. M. Jonas, “Temperature dependence of gating current in myelinated nerve
    fibers,” <i>Journal of Membrane Biology</i>, vol. 112, no. 3. Springer, pp. 277–289,
    1989.
  ista: Jonas PM. 1989. Temperature dependence of gating current in myelinated nerve
    fibers. Journal of Membrane Biology. 112(3), 277–289.
  mla: Jonas, Peter M. “Temperature Dependence of Gating Current in Myelinated Nerve
    Fibers.” <i>Journal of Membrane Biology</i>, vol. 112, no. 3, Springer, 1989,
    pp. 277–89, doi:<a href="https://doi.org/10.1007/BF01870958">10.1007/BF01870958</a>.
  short: P.M. Jonas, Journal of Membrane Biology 112 (1989) 277–289.
date_created: 2018-12-11T12:03:28Z
date_published: 1989-12-01T00:00:00Z
date_updated: 2022-02-15T08:59:02Z
day: '01'
doi: 10.1007/BF01870958
extern: '1'
external_id:
  pmid:
  - '2559205 '
intvolume: '       112'
issue: '3'
language:
- iso: eng
main_file_link:
- url: https://link.springer.com/article/10.1007/BF01870958
month: '12'
oa_version: None
page: 277 - 289
pmid: 1
publication: Journal of Membrane Biology
publication_identifier:
  eissn:
  - 1432-1424
  issn:
  - 0022-2631
publication_status: published
publisher: Springer
publist_id: '2922'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Temperature dependence of gating current in myelinated nerve fibers
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 112
year: '1989'
...