---
_id: '12567'
abstract:
- lang: eng
  text: Single-molecule localization microscopy (SMLM) greatly advances structural
    studies of diverse biological tissues. For example, presynaptic active zone (AZ)
    nanotopology is resolved in increasing detail. Immunofluorescence imaging of AZ
    proteins usually relies on epitope preservation using aldehyde-based immunocompetent
    fixation. Cryofixation techniques, such as high-pressure freezing (HPF) and freeze
    substitution (FS), are widely used for ultrastructural studies of presynaptic
    architecture in electron microscopy (EM). HPF/FS demonstrated nearer-to-native
    preservation of AZ ultrastructure, e.g., by facilitating single filamentous structures.
    Here, we present a protocol combining the advantages of HPF/FS and direct stochastic
    optical reconstruction microscopy (dSTORM) to quantify nanotopology of the AZ
    scaffold protein Bruchpilot (Brp) at neuromuscular junctions (NMJs) of Drosophila
    melanogaster. Using this standardized model, we tested for preservation of Brp
    clusters in different FS protocols compared to classical aldehyde fixation. In
    HPF/FS samples, presynaptic boutons were structurally well preserved with ~22%
    smaller Brp clusters that allowed quantification of subcluster topology. In summary,
    we established a standardized near-to-native preparation and immunohistochemistry
    protocol for SMLM analyses of AZ protein clusters in a defined model synapse.
    Our protocol could be adapted to study protein arrangements at single-molecule
    resolution in other intact tissue preparations.
acknowledgement: This work has been supported by funding of the German Research Foundation
  (Deutsche Forschungsgemeinschaft [DFG], CRC 166, Project B06 to M.H. and A.-L.S.,
  FOR 3004 SYNABS P1 to M.H.) and by the Interdisciplinary Clinical Research Center
  (IZKF) Würzburg (Z-3/69 to M.M.P., N-229 to M.H. and A.-L.S.). A.M. is funded by
  the University of Leipzig Clinician Scientist Program.
article_number: '2128'
article_processing_charge: No
article_type: original
author:
- first_name: Achmed
  full_name: Mrestani, Achmed
  last_name: Mrestani
- first_name: Katharina
  full_name: Lichter, Katharina
  id: 39302e62-fcfc-11ec-8196-8b01447dbd3d
  last_name: Lichter
- first_name: Anna Leena
  full_name: Sirén, Anna Leena
  last_name: Sirén
- first_name: Manfred
  full_name: Heckmann, Manfred
  last_name: Heckmann
- first_name: Mila M.
  full_name: Paul, Mila M.
  last_name: Paul
- first_name: Martin
  full_name: Pauli, Martin
  last_name: Pauli
citation:
  ama: Mrestani A, Lichter K, Sirén AL, Heckmann M, Paul MM, Pauli M. Single-molecule
    localization microscopy of presynaptic active zones in Drosophila melanogaster
    after rapid cryofixation. <i>International Journal of Molecular Sciences</i>.
    2023;24(3). doi:<a href="https://doi.org/10.3390/ijms24032128">10.3390/ijms24032128</a>
  apa: Mrestani, A., Lichter, K., Sirén, A. L., Heckmann, M., Paul, M. M., &#38; Pauli,
    M. (2023). Single-molecule localization microscopy of presynaptic active zones
    in Drosophila melanogaster after rapid cryofixation. <i>International Journal
    of Molecular Sciences</i>. MDPI. <a href="https://doi.org/10.3390/ijms24032128">https://doi.org/10.3390/ijms24032128</a>
  chicago: Mrestani, Achmed, Katharina Lichter, Anna Leena Sirén, Manfred Heckmann,
    Mila M. Paul, and Martin Pauli. “Single-Molecule Localization Microscopy of Presynaptic
    Active Zones in Drosophila Melanogaster after Rapid Cryofixation.” <i>International
    Journal of Molecular Sciences</i>. MDPI, 2023. <a href="https://doi.org/10.3390/ijms24032128">https://doi.org/10.3390/ijms24032128</a>.
  ieee: A. Mrestani, K. Lichter, A. L. Sirén, M. Heckmann, M. M. Paul, and M. Pauli,
    “Single-molecule localization microscopy of presynaptic active zones in Drosophila
    melanogaster after rapid cryofixation,” <i>International Journal of Molecular
    Sciences</i>, vol. 24, no. 3. MDPI, 2023.
  ista: Mrestani A, Lichter K, Sirén AL, Heckmann M, Paul MM, Pauli M. 2023. Single-molecule
    localization microscopy of presynaptic active zones in Drosophila melanogaster
    after rapid cryofixation. International Journal of Molecular Sciences. 24(3),
    2128.
  mla: Mrestani, Achmed, et al. “Single-Molecule Localization Microscopy of Presynaptic
    Active Zones in Drosophila Melanogaster after Rapid Cryofixation.” <i>International
    Journal of Molecular Sciences</i>, vol. 24, no. 3, 2128, MDPI, 2023, doi:<a href="https://doi.org/10.3390/ijms24032128">10.3390/ijms24032128</a>.
  short: A. Mrestani, K. Lichter, A.L. Sirén, M. Heckmann, M.M. Paul, M. Pauli, International
    Journal of Molecular Sciences 24 (2023).
date_created: 2023-02-19T23:00:56Z
date_published: 2023-01-21T00:00:00Z
date_updated: 2023-08-01T13:16:36Z
day: '21'
ddc:
- '570'
department:
- _id: PeJo
doi: 10.3390/ijms24032128
external_id:
  isi:
  - '000930324700001'
file:
- access_level: open_access
  checksum: 69a35dcd3e0249f902ab881b06ee2e58
  content_type: application/pdf
  creator: dernst
  date_created: 2023-02-20T07:09:27Z
  date_updated: 2023-02-20T07:09:27Z
  file_id: '12569'
  file_name: 2023_IJMS_Mrestani.pdf
  file_size: 2823025
  relation: main_file
  success: 1
file_date_updated: 2023-02-20T07:09:27Z
has_accepted_license: '1'
intvolume: '        24'
isi: 1
issue: '3'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Single-molecule localization microscopy of presynaptic active zones in Drosophila
  melanogaster after rapid cryofixation
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 24
year: '2023'
...
---
_id: '9332'
abstract:
- lang: eng
  text: Lateral root (LR) formation is an example of a plant post-embryonic organogenesis
    event. LRs are issued from non-dividing cells entering consecutive steps of formative
    divisions, proliferation and elongation. The chromatin remodeling protein PICKLE
    (PKL) negatively regulates auxin-mediated LR formation through a mechanism that
    is not yet known. Here we show that PKL interacts with RETINOBLASTOMA-RELATED
    1 (RBR1) to repress the LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16) promoter activity.
    Since LBD16 function is required for the formative division of LR founder cells,
    repression mediated by the PKL–RBR1 complex negatively regulates formative division
    and LR formation. Inhibition of LR formation by PKL–RBR1 is counteracted by auxin,
    indicating that, in addition to auxin-mediated transcriptional responses, the
    fine-tuned process of LR formation is also controlled at the chromatin level in
    an auxin-signaling dependent manner.
acknowledgement: "This research was supported by a postdoctoral fellowship of the
  Carl Tryggers Foundation (to K.Ö.) and by grants from Vetenskapsrådet (Nr.: 621-2004-2921
  to L.B.) and VINNOVA (to L.B. and S.R.).\r\nWe thank Frederic Berger, Hidehiro Fukaki,
  Malcolm Bennett, Claudia Köhler, Jiri Friml for providing pRBR1::RBR1-RFP, ssl2-1,
  slr-1, pPKL::PKL-GFP seeds and the DR5 expressing vector, respectively. Authors
  are grateful to Hayashi Kenichiro for providing the auxinol compound and to Rishi
  Bhalerao for stimulating discussions. The technical help of Adeline Rigal and Thomas
  Vain with the auxinol experiments is much appreciated."
article_number: '3862'
article_processing_charge: No
article_type: original
author:
- first_name: Krisztina
  full_name: Ötvös, Krisztina
  id: 29B901B0-F248-11E8-B48F-1D18A9856A87
  last_name: Ötvös
  orcid: 0000-0002-5503-4983
- first_name: Pál
  full_name: Miskolczi, Pál
  last_name: Miskolczi
- first_name: Peter
  full_name: Marhavý, Peter
  id: 3F45B078-F248-11E8-B48F-1D18A9856A87
  last_name: Marhavý
  orcid: 0000-0001-5227-5741
- first_name: Alfredo
  full_name: Cruz-Ramírez, Alfredo
  last_name: Cruz-Ramírez
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Stéphanie
  full_name: Robert, Stéphanie
  last_name: Robert
- first_name: László
  full_name: Bakó, László
  last_name: Bakó
citation:
  ama: Ötvös K, Miskolczi P, Marhavý P, et al. Pickle recruits retinoblastoma related
    1 to control lateral root formation in arabidopsis. <i>International Journal of
    Molecular Sciences</i>. 2021;22(8). doi:<a href="https://doi.org/10.3390/ijms22083862">10.3390/ijms22083862</a>
  apa: Ötvös, K., Miskolczi, P., Marhavý, P., Cruz-Ramírez, A., Benková, E., Robert,
    S., &#38; Bakó, L. (2021). Pickle recruits retinoblastoma related 1 to control
    lateral root formation in arabidopsis. <i>International Journal of Molecular Sciences</i>.
    MDPI. <a href="https://doi.org/10.3390/ijms22083862">https://doi.org/10.3390/ijms22083862</a>
  chicago: Ötvös, Krisztina, Pál Miskolczi, Peter Marhavý, Alfredo Cruz-Ramírez, Eva
    Benková, Stéphanie Robert, and László Bakó. “Pickle Recruits Retinoblastoma Related
    1 to Control Lateral Root Formation in Arabidopsis.” <i>International Journal
    of Molecular Sciences</i>. MDPI, 2021. <a href="https://doi.org/10.3390/ijms22083862">https://doi.org/10.3390/ijms22083862</a>.
  ieee: K. Ötvös <i>et al.</i>, “Pickle recruits retinoblastoma related 1 to control
    lateral root formation in arabidopsis,” <i>International Journal of Molecular
    Sciences</i>, vol. 22, no. 8. MDPI, 2021.
  ista: Ötvös K, Miskolczi P, Marhavý P, Cruz-Ramírez A, Benková E, Robert S, Bakó
    L. 2021. Pickle recruits retinoblastoma related 1 to control lateral root formation
    in arabidopsis. International Journal of Molecular Sciences. 22(8), 3862.
  mla: Ötvös, Krisztina, et al. “Pickle Recruits Retinoblastoma Related 1 to Control
    Lateral Root Formation in Arabidopsis.” <i>International Journal of Molecular
    Sciences</i>, vol. 22, no. 8, 3862, MDPI, 2021, doi:<a href="https://doi.org/10.3390/ijms22083862">10.3390/ijms22083862</a>.
  short: K. Ötvös, P. Miskolczi, P. Marhavý, A. Cruz-Ramírez, E. Benková, S. Robert,
    L. Bakó, International Journal of Molecular Sciences 22 (2021).
date_created: 2021-04-18T22:01:41Z
date_published: 2021-04-08T00:00:00Z
date_updated: 2023-08-08T13:09:58Z
day: '08'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.3390/ijms22083862
external_id:
  isi:
  - '000644394800001'
file:
- access_level: open_access
  checksum: 26ada2531ad1f9c01a1664de0431f1fe
  content_type: application/pdf
  creator: dernst
  date_created: 2021-04-19T10:54:55Z
  date_updated: 2021-04-19T10:54:55Z
  file_id: '9342'
  file_name: 2021_JourMolecularScience_Oetvoes.pdf
  file_size: 2769717
  relation: main_file
  success: 1
file_date_updated: 2021-04-19T10:54:55Z
has_accepted_license: '1'
intvolume: '        22'
isi: 1
issue: '8'
language:
- iso: eng
month: '04'
oa: 1
oa_version: Published Version
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
  issn:
  - 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Pickle recruits retinoblastoma related 1 to control lateral root formation
  in arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 22
year: '2021'
...
---
_id: '9986'
abstract:
- lang: eng
  text: Size control is a fundamental question in biology, showing incremental complexity
    in plants, whose cells possess a rigid cell wall. The phytohormone auxin is a
    vital growth regulator with central importance for differential growth control.
    Our results indicate that auxin-reliant growth programs affect the molecular complexity
    of xyloglucans, the major type of cell wall hemicellulose in eudicots. Auxin-dependent
    induction and repression of growth coincide with reduced and enhanced molecular
    complexity of xyloglucans, respectively. In agreement with a proposed function
    in growth control, genetic interference with xyloglucan side decorations distinctly
    modulates auxin-dependent differential growth rates. Our work proposes that auxin-dependent
    growth programs have a spatially defined effect on xyloglucan’s molecular structure,
    which in turn affects cell wall mechanics and specifies differential, gravitropic
    hypocotyl growth.
acknowledgement: "We are grateful to Paul Knox, Markus Pauly, Malcom O’Neill, and
  Ignacio Zarra for providing published material; the BOKU-VIBT Imaging Center for
  access and M. Debreczeny for expertise; J.I. Thaker and Georg Seifert for critical
  reading.\r\n"
article_number: '9222'
article_processing_charge: Yes
article_type: original
author:
- first_name: Silvia Melina
  full_name: Velasquez, Silvia Melina
  last_name: Velasquez
- first_name: Xiaoyuan
  full_name: Guo, Xiaoyuan
  last_name: Guo
- first_name: Marçal
  full_name: Gallemi, Marçal
  id: 460C6802-F248-11E8-B48F-1D18A9856A87
  last_name: Gallemi
  orcid: 0000-0003-4675-6893
- first_name: Bibek
  full_name: Aryal, Bibek
  last_name: Aryal
- first_name: Peter
  full_name: Venhuizen, Peter
  last_name: Venhuizen
- first_name: Elke
  full_name: Barbez, Elke
  last_name: Barbez
- first_name: Kai Alexander
  full_name: Dünser, Kai Alexander
  last_name: Dünser
- first_name: Martin
  full_name: Darino, Martin
  last_name: Darino
- first_name: Aleš
  full_name: Pӗnčík, Aleš
  last_name: Pӗnčík
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Maria
  full_name: Kalyna, Maria
  last_name: Kalyna
- first_name: Gregory
  full_name: Mouille, Gregory
  last_name: Mouille
- first_name: Eva
  full_name: Benková, Eva
  id: 38F4F166-F248-11E8-B48F-1D18A9856A87
  last_name: Benková
  orcid: 0000-0002-8510-9739
- first_name: Rishikesh P.
  full_name: Bhalerao, Rishikesh P.
  last_name: Bhalerao
- first_name: Jozef
  full_name: Mravec, Jozef
  last_name: Mravec
- first_name: Jürgen
  full_name: Kleine-Vehn, Jürgen
  last_name: Kleine-Vehn
citation:
  ama: Velasquez SM, Guo X, Gallemi M, et al. Xyloglucan remodeling defines auxin-dependent
    differential tissue expansion in plants. <i>International Journal of Molecular
    Sciences</i>. 2021;22(17). doi:<a href="https://doi.org/10.3390/ijms22179222">10.3390/ijms22179222</a>
  apa: Velasquez, S. M., Guo, X., Gallemi, M., Aryal, B., Venhuizen, P., Barbez, E.,
    … Kleine-Vehn, J. (2021). Xyloglucan remodeling defines auxin-dependent differential
    tissue expansion in plants. <i>International Journal of Molecular Sciences</i>.
    MDPI. <a href="https://doi.org/10.3390/ijms22179222">https://doi.org/10.3390/ijms22179222</a>
  chicago: Velasquez, Silvia Melina, Xiaoyuan Guo, Marçal Gallemi, Bibek Aryal, Peter
    Venhuizen, Elke Barbez, Kai Alexander Dünser, et al. “Xyloglucan Remodeling Defines
    Auxin-Dependent Differential Tissue Expansion in Plants.” <i>International Journal
    of Molecular Sciences</i>. MDPI, 2021. <a href="https://doi.org/10.3390/ijms22179222">https://doi.org/10.3390/ijms22179222</a>.
  ieee: S. M. Velasquez <i>et al.</i>, “Xyloglucan remodeling defines auxin-dependent
    differential tissue expansion in plants,” <i>International Journal of Molecular
    Sciences</i>, vol. 22, no. 17. MDPI, 2021.
  ista: Velasquez SM, Guo X, Gallemi M, Aryal B, Venhuizen P, Barbez E, Dünser KA,
    Darino M, Pӗnčík A, Novák O, Kalyna M, Mouille G, Benková E, Bhalerao RP, Mravec
    J, Kleine-Vehn J. 2021. Xyloglucan remodeling defines auxin-dependent differential
    tissue expansion in plants. International Journal of Molecular Sciences. 22(17),
    9222.
  mla: Velasquez, Silvia Melina, et al. “Xyloglucan Remodeling Defines Auxin-Dependent
    Differential Tissue Expansion in Plants.” <i>International Journal of Molecular
    Sciences</i>, vol. 22, no. 17, 9222, MDPI, 2021, doi:<a href="https://doi.org/10.3390/ijms22179222">10.3390/ijms22179222</a>.
  short: S.M. Velasquez, X. Guo, M. Gallemi, B. Aryal, P. Venhuizen, E. Barbez, K.A.
    Dünser, M. Darino, A. Pӗnčík, O. Novák, M. Kalyna, G. Mouille, E. Benková, R.P.
    Bhalerao, J. Mravec, J. Kleine-Vehn, International Journal of Molecular Sciences
    22 (2021).
date_created: 2021-09-05T22:01:24Z
date_published: 2021-08-26T00:00:00Z
date_updated: 2023-10-31T19:29:38Z
day: '26'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.3390/ijms22179222
external_id:
  isi:
  - '000694347100001'
  pmid:
  - '34502129'
file:
- access_level: open_access
  checksum: 6b7055cf89f1b7ed8594c3fdf56f000b
  content_type: application/pdf
  creator: cchlebak
  date_created: 2021-09-06T12:50:19Z
  date_updated: 2021-09-07T09:04:53Z
  file_id: '9988'
  file_name: 2021_IntJMolecularSciences_Velasquez.pdf
  file_size: 2162247
  relation: main_file
file_date_updated: 2021-09-07T09:04:53Z
has_accepted_license: '1'
intvolume: '        22'
isi: 1
issue: '17'
keyword:
- auxin
- growth
- cell wall
- xyloglucans
- hypocotyls
- gravitropism
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
  issn:
  - 1661-6596
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Xyloglucan remodeling defines auxin-dependent differential tissue expansion
  in plants
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 22
year: '2021'
...
---
_id: '6627'
abstract:
- lang: eng
  text: Cortical microtubule arrays in elongating epidermal cells in both the root
    and stem of plants have the propensity of dynamic reorientations that are correlated
    with the activation or inhibition of growth. Factors regulating plant growth,
    among them the hormone auxin, have been recognized as regulators of microtubule
    array orientations. Some previous work in the field has aimed at elucidating the
    causal relationship between cell growth, the signaling of auxin or other growth-regulating
    factors, and microtubule array reorientations, with various conclusions. Here,
    we revisit this problem of causality with a comprehensive set of experiments in
    Arabidopsis thaliana, using the now available pharmacological and genetic tools.
    We use isolated, auxin-depleted hypocotyls, an experimental system allowing for
    full control of both growth and auxin signaling. We demonstrate that reorientation
    of microtubules is not directly triggered by an auxin signal during growth activation.
    Instead, reorientation is triggered by the activation of the growth process itself
    and is auxin-independent in its nature. We discuss these findings in the context
    of previous relevant work, including that on the mechanical regulation of microtubule
    array orientation.
article_number: '3337'
article_processing_charge: Yes
article_type: original
author:
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Adamowski M, Li L, Friml J. Reorientation of cortical microtubule arrays in
    the hypocotyl of arabidopsis thaliana is induced by the cell growth process and
    independent of auxin signaling. <i>International Journal of Molecular Sciences</i>.
    2019;20(13). doi:<a href="https://doi.org/10.3390/ijms20133337">10.3390/ijms20133337</a>
  apa: Adamowski, M., Li, L., &#38; Friml, J. (2019). Reorientation of cortical microtubule
    arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth
    process and independent of auxin signaling. <i>International Journal of Molecular
    Sciences</i>. MDPI. <a href="https://doi.org/10.3390/ijms20133337">https://doi.org/10.3390/ijms20133337</a>
  chicago: Adamowski, Maciek, Lanxin Li, and Jiří Friml. “Reorientation of Cortical
    Microtubule Arrays in the Hypocotyl of Arabidopsis Thaliana Is Induced by the
    Cell Growth Process and Independent of Auxin Signaling.” <i>International Journal
    of Molecular Sciences</i>. MDPI, 2019. <a href="https://doi.org/10.3390/ijms20133337">https://doi.org/10.3390/ijms20133337</a>.
  ieee: M. Adamowski, L. Li, and J. Friml, “Reorientation of cortical microtubule
    arrays in the hypocotyl of arabidopsis thaliana is induced by the cell growth
    process and independent of auxin signaling,” <i>International Journal of Molecular
    Sciences</i>, vol. 20, no. 13. MDPI, 2019.
  ista: Adamowski M, Li L, Friml J. 2019. Reorientation of cortical microtubule arrays
    in the hypocotyl of arabidopsis thaliana is induced by the cell growth process
    and independent of auxin signaling. International Journal of Molecular Sciences.
    20(13), 3337.
  mla: Adamowski, Maciek, et al. “Reorientation of Cortical Microtubule Arrays in
    the Hypocotyl of Arabidopsis Thaliana Is Induced by the Cell Growth Process and
    Independent of Auxin Signaling.” <i>International Journal of Molecular Sciences</i>,
    vol. 20, no. 13, 3337, MDPI, 2019, doi:<a href="https://doi.org/10.3390/ijms20133337">10.3390/ijms20133337</a>.
  short: M. Adamowski, L. Li, J. Friml, International Journal of Molecular Sciences
    20 (2019).
date_created: 2019-07-11T12:00:32Z
date_published: 2019-07-07T00:00:00Z
date_updated: 2025-05-07T11:12:33Z
day: '07'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/ijms20133337
ec_funded: 1
external_id:
  isi:
  - '000477041100221'
  pmid:
  - '31284661'
file:
- access_level: open_access
  checksum: dd9d1cbb933a72ceb666c9667890ac51
  content_type: application/pdf
  creator: dernst
  date_created: 2019-07-17T06:17:15Z
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has_accepted_license: '1'
intvolume: '        20'
isi: 1
issue: '13'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
  name: IST Austria Open Access Fund
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
publication_status: published
publisher: MDPI
quality_controlled: '1'
related_material:
  record:
  - id: '10083'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Reorientation of cortical microtubule arrays in the hypocotyl of arabidopsis
  thaliana is induced by the cell growth process and independent of auxin signaling
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 20
year: '2019'
...
---
_id: '14'
abstract:
- lang: eng
  text: The intercellular transport of auxin is driven by PIN-formed (PIN) auxin efflux
    carriers. PINs are localized at the plasma membrane (PM) and on constitutively
    recycling endomembrane vesicles. Therefore, PINs can mediate auxin transport either
    by direct translocation across the PM or by pumping auxin into secretory vesicles
    (SVs), leading to its secretory release upon fusion with the PM. Which of these
    two mechanisms dominates is a matter of debate. Here, we addressed the issue with
    a mathematical modeling approach. We demonstrate that the efficiency of secretory
    transport depends on SV size, half-life of PINs on the PM, pH, exocytosis frequency
    and PIN density. 3D structured illumination microscopy (SIM) was used to determine
    PIN density on the PM. Combining this data with published values of the other
    parameters, we show that the transport activity of PINs in SVs would have to be
    at least 1000× greater than on the PM in order to produce a comparable macroscopic
    auxin transport. If both transport mechanisms operated simultaneously and PINs
    were equally active on SVs and PM, the contribution of secretion to the total
    auxin flux would be negligible. In conclusion, while secretory vesicle-mediated
    transport of auxin is an intriguing and theoretically possible model, it is unlikely
    to be a major mechanism of auxin transport inplanta.
acknowledgement: 'European Research Council (ERC): 742985 to Jiri Friml; M.A. was
  supported by the Austrian Science Fund (FWF) (M2379-B28); AJ was supported by the
  Austria Science Fund (FWF): I03630 to Jiri Friml.'
article_processing_charge: No
article_type: original
author:
- first_name: Sander
  full_name: Hille, Sander
  last_name: Hille
- first_name: Maria
  full_name: Akhmanova, Maria
  id: 3425EC26-F248-11E8-B48F-1D18A9856A87
  last_name: Akhmanova
  orcid: 0000-0003-1522-3162
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Alexander J
  full_name: Johnson, Alexander J
  id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
  last_name: Johnson
  orcid: 0000-0002-2739-8843
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. Relative contribution
    of PIN-containing secretory vesicles and plasma membrane PINs to the directed
    auxin transport: Theoretical estimation. <i>International Journal of Molecular
    Sciences</i>. 2018;19(11). doi:<a href="https://doi.org/10.3390/ijms19113566">10.3390/ijms19113566</a>'
  apa: 'Hille, S., Akhmanova, M., Glanc, M., Johnson, A. J., &#38; Friml, J. (2018).
    Relative contribution of PIN-containing secretory vesicles and plasma membrane
    PINs to the directed auxin transport: Theoretical estimation. <i>International
    Journal of Molecular Sciences</i>. MDPI. <a href="https://doi.org/10.3390/ijms19113566">https://doi.org/10.3390/ijms19113566</a>'
  chicago: 'Hille, Sander, Maria Akhmanova, Matous Glanc, Alexander J Johnson, and
    Jiří Friml. “Relative Contribution of PIN-Containing Secretory Vesicles and Plasma
    Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.” <i>International
    Journal of Molecular Sciences</i>. MDPI, 2018. <a href="https://doi.org/10.3390/ijms19113566">https://doi.org/10.3390/ijms19113566</a>.'
  ieee: 'S. Hille, M. Akhmanova, M. Glanc, A. J. Johnson, and J. Friml, “Relative
    contribution of PIN-containing secretory vesicles and plasma membrane PINs to
    the directed auxin transport: Theoretical estimation,” <i>International Journal
    of Molecular Sciences</i>, vol. 19, no. 11. MDPI, 2018.'
  ista: 'Hille S, Akhmanova M, Glanc M, Johnson AJ, Friml J. 2018. Relative contribution
    of PIN-containing secretory vesicles and plasma membrane PINs to the directed
    auxin transport: Theoretical estimation. International Journal of Molecular Sciences.
    19(11).'
  mla: 'Hille, Sander, et al. “Relative Contribution of PIN-Containing Secretory Vesicles
    and Plasma Membrane PINs to the Directed Auxin Transport: Theoretical Estimation.”
    <i>International Journal of Molecular Sciences</i>, vol. 19, no. 11, MDPI, 2018,
    doi:<a href="https://doi.org/10.3390/ijms19113566">10.3390/ijms19113566</a>.'
  short: S. Hille, M. Akhmanova, M. Glanc, A.J. Johnson, J. Friml, International Journal
    of Molecular Sciences 19 (2018).
date_created: 2018-12-11T11:44:09Z
date_published: 2018-11-12T00:00:00Z
date_updated: 2023-09-18T08:09:32Z
day: '12'
ddc:
- '580'
department:
- _id: DaSi
- _id: JiFr
doi: 10.3390/ijms19113566
ec_funded: 1
external_id:
  isi:
  - '000451528500282'
file:
- access_level: open_access
  checksum: e4b59c2599b0ca26ebf5b8434bcde94a
  content_type: application/pdf
  creator: dernst
  date_created: 2018-12-17T16:04:11Z
  date_updated: 2020-07-14T12:44:50Z
  file_id: '5719'
  file_name: 2018_IJMS_Hille.pdf
  file_size: 2200593
  relation: main_file
file_date_updated: 2020-07-14T12:44:50Z
has_accepted_license: '1'
intvolume: '        19'
isi: 1
issue: '11'
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742985'
  name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 26538374-B435-11E9-9278-68D0E5697425
  call_identifier: FWF
  grant_number: I03630
  name: Molecular mechanisms of endocytic cargo recognition in plants
publication: International Journal of Molecular Sciences
publication_identifier:
  eissn:
  - 1422-0067
publication_status: published
publisher: MDPI
publist_id: '8042'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Relative contribution of PIN-containing secretory vesicles and plasma membrane
  PINs to the directed auxin transport: Theoretical estimation'
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 19
year: '2018'
...