---
_id: '6262'
abstract:
- lang: eng
  text: "Gravitropism is an adaptive response that orients plant growth parallel to
    the gravity vector. Asymmetric\r\ndistribution of the phytohormone auxin is a
    necessary prerequisite to the tropic bending both in roots and\r\nshoots. During
    hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing\r\ncells
    to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization
    to the bottom cell mem-\r\nbranes leads to the auxin accumulation at the lower
    side of the organ, initiating bending and, later, auxin\r\nfeedback-mediated repolarization
    restores symmetric auxin distribution to terminate bending. Here, we per-\r\nformed
    a forward genetic screen to identify regulators of both PIN3 polarization events
    during gravitropic\r\nresponse. We searched for mutants with defective PIN3 polarizations
    based on easy-to-score morphological\r\noutputs of decreased or increased gravity-induced
    hypocotyl bending. We identified the number of\r\nhypocotyl reduced bending (hrb)
    and hypocotyl hyperbending (hhb) mutants, revealing that reduced bending corre-\r\nlated
    typically with defective gravity-induced PIN3 relocation whereas all analyzed
    hhb mutants showed\r\ndefects in the second, auxin-mediated PIN3 relocation. Next-generation
    sequencing-aided mutation map-\r\nping identified several candidate genes, including
    SCARECROW and ACTIN2, revealing roles of endodermis\r\nspecification and actin
    cytoskeleton in the respective gravity- and auxin-induced PIN polarization events.\r\nThe
    hypocotyl gravitropism screen thus promises to provide novel insights into mechanisms
    underlying cell\r\npolarity and plant adaptive development."
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Hana
  full_name: Rakusová, Hana
  last_name: Rakusová
- first_name: Huibin
  full_name: Han, Huibin
  id: 31435098-F248-11E8-B48F-1D18A9856A87
  last_name: Han
- first_name: Petr
  full_name: Valošek, Petr
  id: 3CDB6F94-F248-11E8-B48F-1D18A9856A87
  last_name: Valošek
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Rakusová H, Han H, Valošek P, Friml J. Genetic screen for factors mediating
    PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. <i>The Plant
    Journal</i>. 2019;98(6):1048-1059. doi:<a href="https://doi.org/10.1111/tpj.14301">10.1111/tpj.14301</a>
  apa: Rakusová, H., Han, H., Valošek, P., &#38; Friml, J. (2019). Genetic screen
    for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana
    hypocotyls. <i>The Plant Journal</i>. Wiley. <a href="https://doi.org/10.1111/tpj.14301">https://doi.org/10.1111/tpj.14301</a>
  chicago: Rakusová, Hana, Huibin Han, Petr Valošek, and Jiří Friml. “Genetic Screen
    for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana
    Hypocotyls.” <i>The Plant Journal</i>. Wiley, 2019. <a href="https://doi.org/10.1111/tpj.14301">https://doi.org/10.1111/tpj.14301</a>.
  ieee: H. Rakusová, H. Han, P. Valošek, and J. Friml, “Genetic screen for factors
    mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls,”
    <i>The Plant Journal</i>, vol. 98, no. 6. Wiley, pp. 1048–1059, 2019.
  ista: Rakusová H, Han H, Valošek P, Friml J. 2019. Genetic screen for factors mediating
    PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant
    Journal. 98(6), 1048–1059.
  mla: Rakusová, Hana, et al. “Genetic Screen for Factors Mediating PIN Polarization
    in Gravistimulated Arabidopsis Thaliana Hypocotyls.” <i>The Plant Journal</i>,
    vol. 98, no. 6, Wiley, 2019, pp. 1048–59, doi:<a href="https://doi.org/10.1111/tpj.14301">10.1111/tpj.14301</a>.
  short: H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal 98 (2019) 1048–1059.
date_created: 2019-04-09T08:46:44Z
date_published: 2019-06-01T00:00:00Z
date_updated: 2025-05-07T11:12:30Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1111/tpj.14301
ec_funded: 1
external_id:
  isi:
  - '000473644100008'
  pmid:
  - '30821050'
file:
- access_level: open_access
  checksum: ad3b5e270b67ba2a45f894ce3be27920
  content_type: application/pdf
  creator: dernst
  date_created: 2019-04-15T09:38:43Z
  date_updated: 2020-07-14T12:47:25Z
  file_id: '6304'
  file_name: 2019_PlantJournal_Rakusov.pdf
  file_size: 1383100
  relation: main_file
file_date_updated: 2020-07-14T12:47:25Z
has_accepted_license: '1'
intvolume: '        98'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1048-1059
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: The Plant Journal
publication_identifier:
  eissn:
  - 1365-313x
  issn:
  - 0960-7412
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis
  thaliana hypocotyls
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 98
year: '2019'
...