---
_id: '9955'
abstract:
- lang: eng
  text: Neurons can change their classical neurotransmitters during ontogeny, sometimes
    going through stages of dual release. Here, we explored the development of the
    neurotransmitter identity of neurons of the avian nucleus isthmi parvocellularis
    (Ipc), whose axon terminals are retinotopically arranged in the optic tectum (TeO)
    and exert a focal gating effect upon the ascending transmission of retinal inputs.
    Although cholinergic and glutamatergic markers are both found in Ipc neurons and
    terminals of adult pigeons and chicks, the mRNA expression of the vesicular acetylcholine
    transporter, VAChT, is weak or absent. To explore how the Ipc neurotransmitter
    identity is established during ontogeny, we analyzed the expression of mRNAs coding
    for cholinergic (ChAT, VAChT, and CHT) and glutamatergic (VGluT2 and VGluT3) markers
    in chick embryos at different developmental stages. We found that between E12
    and E18, Ipc neurons expressed all cholinergic mRNAs and also VGluT2 mRNA; however,
    from E16 through posthatch stages, VAChT mRNA expression was specifically diminished.
    Our ex vivo deposits of tracer crystals and intracellular filling experiments
    revealed that Ipc axons exhibit a mature paintbrush morphology late in development,
    experiencing marked morphological transformations during the period of presumptive
    dual vesicular transmitter release. Additionally, although ChAT protein immunoassays
    increasingly label the growing Ipc axon, this labeling was consistently restricted
    to sparse portions of the terminal branches. Combined, these results suggest that
    the synthesis of glutamate and acetylcholine, and their vesicular release, is
    complexly linked to the developmental processes of branching, growing and remodeling
    of these unique axons.
acknowledgement: 'This work was supported by FONDECYT grants 1151432 and 1210169 to
  Gonzalo J. Marín. FONDECYT grant 1210069 to Jorge Mpodozis. Spanish Ministry of
  Science, Innovation and Universities (MCIU), State Research Agency (AEI) and European
  Regional Development Fund (FEDER), PGC2018-098229-B-100 to José L Ferrán. Spanish
  Ministry of Economy and Competitiveness Excellency Grant BFU2014-57516P (with European
  Community FEDER support), and a Seneca Foundation (Autonomous Community of Murcia)
  Excellency Research contract, ref: 19904/ GERM/15; project name: Genoarchitectonic
  Brain Development and Applications to Neurodegenerative Diseases and Cancer (5672
  Fundación Séneca) to Luis Puelles. The authors gratefully acknowledge the valuable
  editorial help provided by Sara Fernández-Collemann. The authors also thank Elisa
  Sentis and Solano Henríquez for expert technical help.'
article_processing_charge: No
article_type: original
author:
- first_name: Rosana
  full_name: Reyes‐Pinto, Rosana
  last_name: Reyes‐Pinto
- first_name: José L.
  full_name: Ferrán, José L.
  last_name: Ferrán
- first_name: Tomas A
  full_name: Vega Zuniga, Tomas A
  id: 2E7C4E78-F248-11E8-B48F-1D18A9856A87
  last_name: Vega Zuniga
- first_name: Cristian
  full_name: González‐Cabrera, Cristian
  last_name: González‐Cabrera
- first_name: Harald
  full_name: Luksch, Harald
  last_name: Luksch
- first_name: Jorge
  full_name: Mpodozis, Jorge
  last_name: Mpodozis
- first_name: Luis
  full_name: Puelles, Luis
  last_name: Puelles
- first_name: Gonzalo J.
  full_name: Marín, Gonzalo J.
  last_name: Marín
citation:
  ama: Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, et al. Change in the neurochemical
    signature and morphological development of the parvocellular isthmic projection
    to the avian tectum. <i>Journal of Comparative Neurology</i>. 2022;530(2):553-573.
    doi:<a href="https://doi.org/10.1002/cne.25229">10.1002/cne.25229</a>
  apa: Reyes‐Pinto, R., Ferrán, J. L., Vega Zuniga, T. A., González‐Cabrera, C., Luksch,
    H., Mpodozis, J., … Marín, G. J. (2022). Change in the neurochemical signature
    and morphological development of the parvocellular isthmic projection to the avian
    tectum. <i>Journal of Comparative Neurology</i>. Wiley. <a href="https://doi.org/10.1002/cne.25229">https://doi.org/10.1002/cne.25229</a>
  chicago: Reyes‐Pinto, Rosana, José L. Ferrán, Tomas A Vega Zuniga, Cristian González‐Cabrera,
    Harald Luksch, Jorge Mpodozis, Luis Puelles, and Gonzalo J. Marín. “Change in
    the Neurochemical Signature and Morphological Development of the Parvocellular
    Isthmic Projection to the Avian Tectum.” <i>Journal of Comparative Neurology</i>.
    Wiley, 2022. <a href="https://doi.org/10.1002/cne.25229">https://doi.org/10.1002/cne.25229</a>.
  ieee: R. Reyes‐Pinto <i>et al.</i>, “Change in the neurochemical signature and morphological
    development of the parvocellular isthmic projection to the avian tectum,” <i>Journal
    of Comparative Neurology</i>, vol. 530, no. 2. Wiley, pp. 553–573, 2022.
  ista: Reyes‐Pinto R, Ferrán JL, Vega Zuniga TA, González‐Cabrera C, Luksch H, Mpodozis
    J, Puelles L, Marín GJ. 2022. Change in the neurochemical signature and morphological
    development of the parvocellular isthmic projection to the avian tectum. Journal
    of Comparative Neurology. 530(2), 553–573.
  mla: Reyes‐Pinto, Rosana, et al. “Change in the Neurochemical Signature and Morphological
    Development of the Parvocellular Isthmic Projection to the Avian Tectum.” <i>Journal
    of Comparative Neurology</i>, vol. 530, no. 2, Wiley, 2022, pp. 553–73, doi:<a
    href="https://doi.org/10.1002/cne.25229">10.1002/cne.25229</a>.
  short: R. Reyes‐Pinto, J.L. Ferrán, T.A. Vega Zuniga, C. González‐Cabrera, H. Luksch,
    J. Mpodozis, L. Puelles, G.J. Marín, Journal of Comparative Neurology 530 (2022)
    553–573.
date_created: 2021-08-23T08:40:59Z
date_published: 2022-02-01T00:00:00Z
date_updated: 2023-08-11T10:58:17Z
day: '01'
department:
- _id: MaJö
doi: 10.1002/cne.25229
external_id:
  isi:
  - '000686420000001'
  pmid:
  - '34363623'
intvolume: '       530'
isi: 1
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
page: 553-573
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
  eissn:
  - 1096-9861
  issn:
  - 0021-9967
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Change in the neurochemical signature and morphological development of the
  parvocellular isthmic projection to the avian tectum
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 530
year: '2022'
...
---
_id: '7148'
abstract:
- lang: eng
  text: In the cerebellum, GluD2 is exclusively expressed in Purkinje cells, where
    it regulates synapse formation and regeneration, synaptic plasticity, and motor
    learning. Delayed cognitive development in humans with GluD2 gene mutations suggests
    extracerebellar functions of GluD2. However, extracerebellar expression of GluD2
    and its relationship with that of GluD1 are poorly understood. GluD2 mRNA and
    protein were widely detected, with relatively high levels observed in the olfactory
    glomerular layer, medial prefrontal cortex, cingulate cortex, retrosplenial granular
    cortex, olfactory tubercle, subiculum, striatum, lateral septum, anterodorsal
    thalamic nucleus, and arcuate hypothalamic nucleus. These regions were also enriched
    for GluD1, and many individual neurons coexpressed the two GluDs. In the retrosplenial
    granular cortex, GluD1 and GluD2 were selectively expressed at PSD‐95‐expressing
    glutamatergic synapses, and their coexpression on the same synapses was shown
    by SDS‐digested freeze‐fracture replica labeling. Biochemically, GluD1 and GluD2
    formed coimmunoprecipitable complex formation in HEK293T cells and in the cerebral
    cortex and hippocampus. We further estimated the relative protein amount by quantitative
    immunoblotting using GluA2/GluD2 and GluA2/GluD1 chimeric proteins as standards
    for titration of GluD1 and GluD2 antibodies. Intriguingly, the relative amount
    of GluD2 was almost comparable to that of GluD1 in the postsynaptic density fraction
    prepared from the cerebral cortex and hippocampus. In contrast, GluD2 was overwhelmingly
    predominant in the cerebellum. Thus, we have determined the relative extracerebellar
    expression of GluD1 and GluD2 at regional, neuronal, and synaptic levels. These
    data provide a molecular–anatomical basis for possible competitive and cooperative
    interactions of GluD family members at synapses in various brain regions.
acknowledgement: This study was supported by Grants-in-Aid for Scientific Research
  to K.K. (18K06813), Y.M. (17K08503, 17H0631319), and K.S. (16H04650) and a grant
  for Scientific Research on Innovative Areas to K.S (16H06276) from the Ministry
  of Education, Culture, Sports, Science and Technology of Japan (MEXT). We thank
  K. Akashi, I. Watanabe-Iida, Y. Suzuki, and H. Azechi for technical assistance and
  advice, and H. Uchida for valuable discussions. We thank E. Kushiya,I. Yabe, C.
  Ohori, Y. Mochizuki, Y. Ishikawa, and N. Ishimoto for technical assistance in generating
  GluD1-KO mice.
article_processing_charge: No
article_type: original
author:
- first_name: Chihiro
  full_name: Nakamoto, Chihiro
  last_name: Nakamoto
- first_name: Kohtarou
  full_name: Konno, Kohtarou
  last_name: Konno
- first_name: Taisuke
  full_name: Miyazaki, Taisuke
  last_name: Miyazaki
- first_name: Ena
  full_name: Nakatsukasa, Ena
  last_name: Nakatsukasa
- first_name: Rie
  full_name: Natsume, Rie
  last_name: Natsume
- first_name: Manabu
  full_name: Abe, Manabu
  last_name: Abe
- first_name: Meiko
  full_name: Kawamura, Meiko
  last_name: Kawamura
- first_name: Yugo
  full_name: Fukazawa, Yugo
  last_name: Fukazawa
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Miwako
  full_name: Yamasaki, Miwako
  last_name: Yamasaki
- first_name: Kenji
  full_name: Sakimura, Kenji
  last_name: Sakimura
- first_name: Masahiko
  full_name: Watanabe, Masahiko
  last_name: Watanabe
citation:
  ama: Nakamoto C, Konno K, Miyazaki T, et al. Expression mapping, quantification,
    and complex formation of GluD1 and GluD2 glutamate receptors in adult mouse brain.
    <i>Journal of Comparative Neurology</i>. 2020;528(6):1003-1027. doi:<a href="https://doi.org/10.1002/cne.24792">10.1002/cne.24792</a>
  apa: Nakamoto, C., Konno, K., Miyazaki, T., Nakatsukasa, E., Natsume, R., Abe, M.,
    … Watanabe, M. (2020). Expression mapping, quantification, and complex formation
    of GluD1 and GluD2 glutamate receptors in adult mouse brain. <i>Journal of Comparative
    Neurology</i>. Wiley. <a href="https://doi.org/10.1002/cne.24792">https://doi.org/10.1002/cne.24792</a>
  chicago: Nakamoto, Chihiro, Kohtarou Konno, Taisuke Miyazaki, Ena Nakatsukasa, Rie
    Natsume, Manabu Abe, Meiko Kawamura, et al. “Expression Mapping, Quantification,
    and Complex Formation of GluD1 and GluD2 Glutamate Receptors in Adult Mouse Brain.”
    <i>Journal of Comparative Neurology</i>. Wiley, 2020. <a href="https://doi.org/10.1002/cne.24792">https://doi.org/10.1002/cne.24792</a>.
  ieee: C. Nakamoto <i>et al.</i>, “Expression mapping, quantification, and complex
    formation of GluD1 and GluD2 glutamate receptors in adult mouse brain,” <i>Journal
    of Comparative Neurology</i>, vol. 528, no. 6. Wiley, pp. 1003–1027, 2020.
  ista: Nakamoto C, Konno K, Miyazaki T, Nakatsukasa E, Natsume R, Abe M, Kawamura
    M, Fukazawa Y, Shigemoto R, Yamasaki M, Sakimura K, Watanabe M. 2020. Expression
    mapping, quantification, and complex formation of GluD1 and GluD2 glutamate receptors
    in adult mouse brain. Journal of Comparative Neurology. 528(6), 1003–1027.
  mla: Nakamoto, Chihiro, et al. “Expression Mapping, Quantification, and Complex
    Formation of GluD1 and GluD2 Glutamate Receptors in Adult Mouse Brain.” <i>Journal
    of Comparative Neurology</i>, vol. 528, no. 6, Wiley, 2020, pp. 1003–27, doi:<a
    href="https://doi.org/10.1002/cne.24792">10.1002/cne.24792</a>.
  short: C. Nakamoto, K. Konno, T. Miyazaki, E. Nakatsukasa, R. Natsume, M. Abe, M.
    Kawamura, Y. Fukazawa, R. Shigemoto, M. Yamasaki, K. Sakimura, M. Watanabe, Journal
    of Comparative Neurology 528 (2020) 1003–1027.
date_created: 2019-12-04T16:09:29Z
date_published: 2020-04-01T00:00:00Z
date_updated: 2023-08-17T14:06:50Z
day: '01'
ddc:
- '571'
- '599'
department:
- _id: RySh
doi: 10.1002/cne.24792
external_id:
  isi:
  - '000496410200001'
  pmid:
  - '31625608'
has_accepted_license: '1'
intvolume: '       528'
isi: 1
issue: '6'
language:
- iso: eng
month: '04'
oa_version: None
page: 1003-1027
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
  eissn:
  - 1096-9861
  issn:
  - 0021-9967
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Expression mapping, quantification, and complex formation of GluD1 and GluD2
  glutamate receptors in adult mouse brain
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 528
year: '2020'
...