---
_id: '6564'
abstract:
- lang: eng
  text: Optogenetics enables the spatio-temporally precise control of cell and animal
    behavior. Many optogenetic tools are driven by light-controlled protein–protein
    interactions (PPIs) that are repurposed from natural light-sensitive domains (LSDs).
    Applying light-controlled PPIs to new target proteins is challenging because it
    is difficult to predict which of the many available LSDs, if any, will yield robust
    light regulation. As a consequence, fusion protein libraries need to be prepared
    and tested, but methods and platforms to facilitate this process are currently
    not available. Here, we developed a genetic engineering strategy and vector library
    for the rapid generation of light-controlled PPIs. The strategy permits fusing
    a target protein to multiple LSDs efficiently and in two orientations. The public
    and expandable library contains 29 vectors with blue, green or red light-responsive
    LSDs, many of which have been previously applied ex vivo and in vivo. We demonstrate
    the versatility of the approach and the necessity for sampling LSDs by generating
    light-activated caspase-9 (casp9) enzymes. Collectively, this work provides a
    new resource for optical regulation of a broad range of target proteins in cell
    and developmental biology.
article_processing_charge: No
article_type: original
author:
- first_name: Alexandra-Madelaine
  full_name: Tichy, Alexandra-Madelaine
  id: 29D8BB2C-F248-11E8-B48F-1D18A9856A87
  last_name: Tichy
- first_name: Elliot J.
  full_name: Gerrard, Elliot J.
  last_name: Gerrard
- first_name: Julien M.D.
  full_name: Legrand, Julien M.D.
  last_name: Legrand
- first_name: Robin M.
  full_name: Hobbs, Robin M.
  last_name: Hobbs
- first_name: Harald L
  full_name: Janovjak, Harald L
  id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
  last_name: Janovjak
  orcid: 0000-0002-8023-9315
citation:
  ama: Tichy A-M, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak HL. Engineering strategy
    and vector library for the rapid generation of modular light-controlled protein–protein
    interactions. <i>Journal of Molecular Biology</i>. 2019;431(17):3046-3055. doi:<a
    href="https://doi.org/10.1016/j.jmb.2019.05.033">10.1016/j.jmb.2019.05.033</a>
  apa: Tichy, A.-M., Gerrard, E. J., Legrand, J. M. D., Hobbs, R. M., &#38; Janovjak,
    H. L. (2019). Engineering strategy and vector library for the rapid generation
    of modular light-controlled protein–protein interactions. <i>Journal of Molecular
    Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.jmb.2019.05.033">https://doi.org/10.1016/j.jmb.2019.05.033</a>
  chicago: Tichy, Alexandra-Madelaine, Elliot J. Gerrard, Julien M.D. Legrand, Robin
    M. Hobbs, and Harald L Janovjak. “Engineering Strategy and Vector Library for
    the Rapid Generation of Modular Light-Controlled Protein–Protein Interactions.”
    <i>Journal of Molecular Biology</i>. Elsevier, 2019. <a href="https://doi.org/10.1016/j.jmb.2019.05.033">https://doi.org/10.1016/j.jmb.2019.05.033</a>.
  ieee: A.-M. Tichy, E. J. Gerrard, J. M. D. Legrand, R. M. Hobbs, and H. L. Janovjak,
    “Engineering strategy and vector library for the rapid generation of modular light-controlled
    protein–protein interactions,” <i>Journal of Molecular Biology</i>, vol. 431,
    no. 17. Elsevier, pp. 3046–3055, 2019.
  ista: Tichy A-M, Gerrard EJ, Legrand JMD, Hobbs RM, Janovjak HL. 2019. Engineering
    strategy and vector library for the rapid generation of modular light-controlled
    protein–protein interactions. Journal of Molecular Biology. 431(17), 3046–3055.
  mla: Tichy, Alexandra-Madelaine, et al. “Engineering Strategy and Vector Library
    for the Rapid Generation of Modular Light-Controlled Protein–Protein Interactions.”
    <i>Journal of Molecular Biology</i>, vol. 431, no. 17, Elsevier, 2019, pp. 3046–55,
    doi:<a href="https://doi.org/10.1016/j.jmb.2019.05.033">10.1016/j.jmb.2019.05.033</a>.
  short: A.-M. Tichy, E.J. Gerrard, J.M.D. Legrand, R.M. Hobbs, H.L. Janovjak, Journal
    of Molecular Biology 431 (2019) 3046–3055.
date_created: 2019-06-16T21:59:14Z
date_published: 2019-08-09T00:00:00Z
date_updated: 2023-08-28T09:39:22Z
day: '09'
department:
- _id: HaJa
doi: 10.1016/j.jmb.2019.05.033
external_id:
  isi:
  - '000482872100002'
intvolume: '       431'
isi: 1
issue: '17'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: http://www.biorxiv.org/content/10.1101/583369v1
month: '08'
oa: 1
oa_version: Preprint
page: 3046-3055
publication: Journal of Molecular Biology
publication_identifier:
  eissn:
  - '10898638'
  issn:
  - '00222836'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Engineering strategy and vector library for the rapid generation of modular
  light-controlled protein–protein interactions
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 431
year: '2019'
...