[{"publication":"Proceedings of the Royal Society of London Series B Biological Sciences","publist_id":"6060","intvolume":"       283","scopus_import":1,"related_material":{"record":[{"status":"public","relation":"research_data","id":"9704"}]},"doi":"10.1098/rspb.2016.0811","year":"2016","day":"29","acknowledgement":"This work was supported by the Federal Ministry of Food, Agriculture and Consumer Protection (Germany): Fit Bee project (grant 511-06.01-28-1-71.007-10), the EU: BeeDoc (grant 244956), iDiv (2013 NGS-Fast Track grant W47004118) and the Insect Pollinators Initiative (IPI grant BB/I000100/1 and BB/I000151/1). The IPI is funded jointly by the Biotechnology and Biological Sciences Research Council, the Department for Environment, Food and Rural Affairs, the Natural Environment Research Council, the Scottish Government and the Wellcome Trust, under the Living with Environmental Change Partnership. We thank A. Abrahams, M. Husemann and A. Soro\r\nfor support in obtaining\r\nV.  destructor\r\n-free honeybees; and BBKA\r\nPresident D. Aston for access to records of colony overwinter\r\n2011–2012 mortality in the UK. We also thank the anonymous refe-\r\nrees and Stephen Martin for comments that led to substantial\r\nimprovement of the manuscript.","oa":1,"title":"Elevated virulence of an emerging viral genotype as a driver of honeybee loss","publisher":"Royal Society, The","file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-06-29T00:00:00Z","ddc":["576","592"],"publication_status":"published","abstract":[{"text":"Emerging infectious diseases (EIDs) have contributed significantly to the current biodiversity crisis, leading to widespread epidemics and population loss. Owing to genetic variation in pathogen virulence, a complete understanding of species decline requires the accurate identification and characterization of EIDs. We explore this issue in the Western honeybee, where increasing mortality of populations in the Northern Hemisphere has caused major concern. Specifically, we investigate the importance of genetic identity of the main suspect in mortality, deformed wing virus (DWV), in driving honeybee loss. Using laboratory experiments and a systematic field survey, we demonstrate that an emerging DWV genotype (DWV-B) is more virulent than the established DWV genotype (DWV-A) and is widespread in the landscape. Furthermore, we show in a simple model that colonies infected with DWV-B collapse sooner than colonies infected with DWV-A. We also identify potential for rapid DWV evolution by revealing extensive genome-wide recombination in vivo. The emergence of DWV-B in naive honeybee populations, including via recombination with DWV-A, could be of significant ecological and economic importance. Our findings emphasize that knowledge of pathogen genetic identity and diversity is critical to understanding drivers of species decline.","lang":"eng"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"mla":"Mcmahon, Dino, et al. “Elevated Virulence of an Emerging Viral Genotype as a Driver of Honeybee Loss.” <i>Proceedings of the Royal Society of London Series B Biological Sciences</i>, vol. 283, no. 1833, 20160811, Royal Society, The, 2016, doi:<a href=\"https://doi.org/10.1098/rspb.2016.0811\">10.1098/rspb.2016.0811</a>.","short":"D. Mcmahon, M. Natsopoulou, V. Doublet, M. Fürst, S. Weging, M. Brown, A. Gogol Döring, R. Paxton, Proceedings of the Royal Society of London Series B Biological Sciences 283 (2016).","ieee":"D. Mcmahon <i>et al.</i>, “Elevated virulence of an emerging viral genotype as a driver of honeybee loss,” <i>Proceedings of the Royal Society of London Series B Biological Sciences</i>, vol. 283, no. 1833. Royal Society, The, 2016.","apa":"Mcmahon, D., Natsopoulou, M., Doublet, V., Fürst, M., Weging, S., Brown, M., … Paxton, R. (2016). Elevated virulence of an emerging viral genotype as a driver of honeybee loss. <i>Proceedings of the Royal Society of London Series B Biological Sciences</i>. Royal Society, The. <a href=\"https://doi.org/10.1098/rspb.2016.0811\">https://doi.org/10.1098/rspb.2016.0811</a>","ista":"Mcmahon D, Natsopoulou M, Doublet V, Fürst M, Weging S, Brown M, Gogol Döring A, Paxton R. 2016. Elevated virulence of an emerging viral genotype as a driver of honeybee loss. Proceedings of the Royal Society of London Series B Biological Sciences. 283(1833), 20160811.","ama":"Mcmahon D, Natsopoulou M, Doublet V, et al. Elevated virulence of an emerging viral genotype as a driver of honeybee loss. <i>Proceedings of the Royal Society of London Series B Biological Sciences</i>. 2016;283(1833). doi:<a href=\"https://doi.org/10.1098/rspb.2016.0811\">10.1098/rspb.2016.0811</a>","chicago":"Mcmahon, Dino, Myrsini Natsopoulou, Vincent Doublet, Matthias Fürst, Silvio Weging, Mark Brown, Andreas Gogol Döring, and Robert Paxton. “Elevated Virulence of an Emerging Viral Genotype as a Driver of Honeybee Loss.” <i>Proceedings of the Royal Society of London Series B Biological Sciences</i>. Royal Society, The, 2016. <a href=\"https://doi.org/10.1098/rspb.2016.0811\">https://doi.org/10.1098/rspb.2016.0811</a>."},"department":[{"_id":"SyCr"}],"date_updated":"2023-02-23T14:05:30Z","pubrep_id":"701","status":"public","license":"https://creativecommons.org/licenses/by/4.0/","_id":"1262","type":"journal_article","date_created":"2018-12-11T11:51:00Z","volume":283,"language":[{"iso":"eng"}],"file":[{"relation":"main_file","creator":"system","file_id":"4708","checksum":"0b0d1be38b497d004064650acb3baced","file_size":796872,"access_level":"open_access","content_type":"application/pdf","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:08:46Z","file_name":"IST-2016-701-v1+1_20160811.full.pdf"}],"article_number":"20160811","issue":"1833","has_accepted_license":"1","month":"06","author":[{"first_name":"Dino","last_name":"Mcmahon","full_name":"Mcmahon, Dino"},{"first_name":"Myrsini","last_name":"Natsopoulou","full_name":"Natsopoulou, Myrsini"},{"first_name":"Vincent","last_name":"Doublet","full_name":"Doublet, Vincent"},{"orcid":"0000-0002-3712-925X","first_name":"Matthias","last_name":"Fürst","full_name":"Fürst, Matthias","id":"393B1196-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Silvio","last_name":"Weging","full_name":"Weging, Silvio"},{"first_name":"Mark","last_name":"Brown","full_name":"Brown, Mark"},{"last_name":"Gogol Döring","first_name":"Andreas","full_name":"Gogol Döring, Andreas"},{"first_name":"Robert","last_name":"Paxton","full_name":"Paxton, Robert"}],"oa_version":"Published Version","quality_controlled":"1"},{"publisher":"Optica Publishing Group","date_published":"2016-06-20T00:00:00Z","title":"Efficient microwave to optical photon conversion: An electro-optical realization","publication_status":"published","abstract":[{"text":"Linking classical microwave electrical circuits to the optical telecommunication band is at the core of modern communication. Future quantum information networks will require coherent microwave-to-optical conversion to link electronic quantum processors and memories via low-loss optical telecommunication networks. Efficient conversion can be achieved with electro-optical modulators operating at the single microwave photon level. In the standard electro-optic modulation scheme, this is impossible because both up- and down-converted sidebands are necessarily present. Here, we demonstrate true single-sideband up- or down-conversion in a triply resonant whispering gallery mode resonator by explicitly addressing modes with asymmetric free spectral range. Compared to previous experiments, we show a 3 orders of magnitude improvement of the electro-optical conversion efficiency, reaching 0.1% photon number conversion for a 10 GHz microwave tone at 0.42 mW of optical pump power. The presented scheme is fully compatible with existing superconducting 3D circuit quantum electrodynamics technology and can be used for nonclassical state conversion and communication. Our conversion bandwidth is larger than 1 MHz and is not fundamentally limited.","lang":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JoFi"}],"date_updated":"2023-10-17T12:17:15Z","citation":{"ista":"Rueda A, Sedlmeir F, Collodo M, Vogl U, Stiller B, Schunk G, Strekalov D, Marquardt C, Fink JM, Painter O, Leuchs G, Schwefel H. 2016. Efficient microwave to optical photon conversion: An electro-optical realization. Optica. 3(6), 597–604.","ama":"Rueda A, Sedlmeir F, Collodo M, et al. Efficient microwave to optical photon conversion: An electro-optical realization. <i>Optica</i>. 2016;3(6):597-604. doi:<a href=\"https://doi.org/10.1364/OPTICA.3.000597\">10.1364/OPTICA.3.000597</a>","chicago":"Rueda, Alfredo, Florian Sedlmeir, Michele Collodo, Ulrich Vogl, Birgit Stiller, Gerhard Schunk, Dmitry Strekalov, et al. “Efficient Microwave to Optical Photon Conversion: An Electro-Optical Realization.” <i>Optica</i>. Optica Publishing Group, 2016. <a href=\"https://doi.org/10.1364/OPTICA.3.000597\">https://doi.org/10.1364/OPTICA.3.000597</a>.","apa":"Rueda, A., Sedlmeir, F., Collodo, M., Vogl, U., Stiller, B., Schunk, G., … Schwefel, H. (2016). Efficient microwave to optical photon conversion: An electro-optical realization. <i>Optica</i>. Optica Publishing Group. <a href=\"https://doi.org/10.1364/OPTICA.3.000597\">https://doi.org/10.1364/OPTICA.3.000597</a>","ieee":"A. Rueda <i>et al.</i>, “Efficient microwave to optical photon conversion: An electro-optical realization,” <i>Optica</i>, vol. 3, no. 6. Optica Publishing Group, pp. 597–604, 2016.","short":"A. Rueda, F. Sedlmeir, M. Collodo, U. Vogl, B. Stiller, G. Schunk, D. Strekalov, C. Marquardt, J.M. Fink, O. Painter, G. Leuchs, H. Schwefel, Optica 3 (2016) 597–604.","mla":"Rueda, Alfredo, et al. “Efficient Microwave to Optical Photon Conversion: An Electro-Optical Realization.” <i>Optica</i>, vol. 3, no. 6, Optica Publishing Group, 2016, pp. 597–604, doi:<a href=\"https://doi.org/10.1364/OPTICA.3.000597\">10.1364/OPTICA.3.000597</a>."},"publist_id":"6061","intvolume":"         3","publication":"Optica","scopus_import":"1","acknowledgement":"Alexander von Humboldt Foundation; Studienstiftung des Deutschen Volkes. We would like to acknowledge our stimulating discussions with Konrad Lehnert and Alessandro Pitanti.","day":"20","year":"2016","doi":"10.1364/OPTICA.3.000597","page":"597 - 604","oa":1,"language":[{"iso":"eng"}],"volume":3,"article_processing_charge":"No","month":"06","issue":"6","author":[{"last_name":"Rueda","first_name":"Alfredo","full_name":"Rueda, Alfredo"},{"full_name":"Sedlmeir, Florian","first_name":"Florian","last_name":"Sedlmeir"},{"full_name":"Collodo, Michele","last_name":"Collodo","first_name":"Michele"},{"full_name":"Vogl, Ulrich","last_name":"Vogl","first_name":"Ulrich"},{"full_name":"Stiller, Birgit","first_name":"Birgit","last_name":"Stiller"},{"last_name":"Schunk","first_name":"Gerhard","full_name":"Schunk, Gerhard"},{"full_name":"Strekalov, Dmitry","first_name":"Dmitry","last_name":"Strekalov"},{"first_name":"Christoph","last_name":"Marquardt","full_name":"Marquardt, Christoph"},{"full_name":"Fink, Johannes M","id":"4B591CBA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8112-028X","first_name":"Johannes M","last_name":"Fink"},{"first_name":"Oskar","last_name":"Painter","full_name":"Painter, Oskar"},{"first_name":"Gerd","last_name":"Leuchs","full_name":"Leuchs, Gerd"},{"first_name":"Harald","last_name":"Schwefel","full_name":"Schwefel, Harald"}],"quality_controlled":"1","oa_version":"Published Version","main_file_link":[{"url":"https://doi.org/10.1364/OPTICA.3.000597","open_access":"1"}],"status":"public","date_created":"2018-12-11T11:51:01Z","_id":"1263","type":"journal_article"},{"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"date_updated":"2021-01-12T06:49:29Z","citation":{"ieee":"G. Sancho Andrés <i>et al.</i>, “Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier,” <i>Plant Physiology</i>, vol. 171, no. 3. American Society of Plant Biologists, pp. 1965–1982, 2016.","ista":"Sancho Andrés G, Soriano Ortega E, Gao C, Bernabé Orts J, Narasimhan M, Müller A, Tejos R, Jiang L, Friml J, Aniento F, Marcote M. 2016. Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier. Plant Physiology. 171(3), 1965–1982.","chicago":"Sancho Andrés, Gloria, Esther Soriano Ortega, Caiji Gao, Joan Bernabé Orts, Madhumitha Narasimhan, Anna Müller, Ricardo Tejos, et al. “Sorting Motifs Involved in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1104/pp.16.00373\">https://doi.org/10.1104/pp.16.00373</a>.","ama":"Sancho Andrés G, Soriano Ortega E, Gao C, et al. Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier. <i>Plant Physiology</i>. 2016;171(3):1965-1982. doi:<a href=\"https://doi.org/10.1104/pp.16.00373\">10.1104/pp.16.00373</a>","apa":"Sancho Andrés, G., Soriano Ortega, E., Gao, C., Bernabé Orts, J., Narasimhan, M., Müller, A., … Marcote, M. (2016). Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.00373\">https://doi.org/10.1104/pp.16.00373</a>","short":"G. Sancho Andrés, E. Soriano Ortega, C. Gao, J. Bernabé Orts, M. Narasimhan, A. Müller, R. Tejos, L. Jiang, J. Friml, F. Aniento, M. Marcote, Plant Physiology 171 (2016) 1965–1982.","mla":"Sancho Andrés, Gloria, et al. “Sorting Motifs Involved in the Trafficking and Localization of the PIN1 Auxin Efflux Carrier.” <i>Plant Physiology</i>, vol. 171, no. 3, American Society of Plant Biologists, 2016, pp. 1965–82, doi:<a href=\"https://doi.org/10.1104/pp.16.00373\">10.1104/pp.16.00373</a>."},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication_status":"published","abstract":[{"text":"n contrast with the wealth of recent reports about the function of μ-adaptins and clathrin adaptor protein (AP) complexes, there is very little information about the motifs that determine the sorting of membrane proteins within clathrin-coated vesicles in plants. Here, we investigated putative sorting signals in the large cytosolic loop of the Arabidopsis (Arabidopsis thaliana) PIN-FORMED1 (PIN1) auxin transporter, which are involved in binding μ-adaptins and thus in PIN1 trafficking and localization. We found that Phe-165 and Tyr-280, Tyr-328, and Tyr-394 are involved in the binding of different μ-adaptins in vitro. However, only Phe-165, which binds μA(μ2)- and μD(μ3)-adaptin, was found to be essential for PIN1 trafficking and localization in vivo. The PIN1:GFP-F165A mutant showed reduced endocytosis but also localized to intracellular structures containing several layers of membranes and endoplasmic reticulum (ER) markers, suggesting that they correspond to ER or ER-derived membranes. While PIN1:GFP localized normally in a μA (μ2)-adaptin mutant, it accumulated in big intracellular structures containing LysoTracker in a μD (μ3)-adaptin mutant, consistent with previous results obtained with mutants of other subunits of the AP-3 complex. Our data suggest that Phe-165, through the binding of μA (μ2)- and μD (μ3)-adaptin, is important for PIN1 endocytosis and for PIN1 trafficking along the secretory pathway, respectively.","lang":"eng"}],"date_published":"2016-07-01T00:00:00Z","publisher":"American Society of Plant Biologists","title":"Sorting motifs involved in the trafficking and localization of the PIN1 auxin efflux carrier","oa":1,"acknowledgement":"We thank Dr. R. Offringa (Leiden University) for providing the GST-\r\nPIN-CL construct; Sandra Richter and Gerd Jurgens (University of Tübin-\r\ngen) for providing the estradiol-inducible PIN1-RFP construct and the\r\ngnl1 mutant expressing BFA-sensitive GNL1; F.J. Santonja (University of Valencia)\r\nfor help with the statistical analysis; Jurgen Kleine-Vehn, Elke Barbez, and\r\nEva Benkova for helpful discussions; the Salk Institute Genomic Analysis\r\nLaboratory for providing the sequence-indexed Arabidopsis T-DNA in-\r\nsertion mutants; and the greenhouse section and the microscopy section\r\nof SCSIE (University of Valencia) and Pilar Selvi for excellent technical\r\nassistance.","day":"01","doi":"10.1104/pp.16.00373","year":"2016","page":"1965 - 1982","scopus_import":1,"publist_id":"6059","intvolume":"       171","publication":"Plant Physiology","quality_controlled":"1","project":[{"call_identifier":"FP7","name":"Polarity and subcellular dynamics in plants","grant_number":"282300","_id":"25716A02-B435-11E9-9278-68D0E5697425"}],"oa_version":"Submitted Version","author":[{"full_name":"Sancho Andrés, Gloria","first_name":"Gloria","last_name":"Sancho Andrés"},{"full_name":"Soriano Ortega, Esther","last_name":"Soriano Ortega","first_name":"Esther"},{"full_name":"Gao, Caiji","first_name":"Caiji","last_name":"Gao"},{"full_name":"Bernabé Orts, Joan","first_name":"Joan","last_name":"Bernabé Orts"},{"orcid":"0000-0002-8600-0671","last_name":"Narasimhan","first_name":"Madhumitha","full_name":"Narasimhan, Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Anna","last_name":"Müller","id":"420AB15A-F248-11E8-B48F-1D18A9856A87","full_name":"Müller, Anna"},{"full_name":"Tejos, Ricardo","last_name":"Tejos","first_name":"Ricardo"},{"last_name":"Jiang","first_name":"Liwen","full_name":"Jiang, Liwen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596"},{"full_name":"Aniento, Fernando","first_name":"Fernando","last_name":"Aniento"},{"full_name":"Marcote, Maria","last_name":"Marcote","first_name":"Maria"}],"month":"07","issue":"3","language":[{"iso":"eng"}],"volume":171,"date_created":"2018-12-11T11:51:01Z","_id":"1264","type":"journal_article","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4936568/","open_access":"1"}],"status":"public","ec_funded":1},{"date_updated":"2021-01-12T06:49:29Z","department":[{"_id":"EvBe"}],"quality_controlled":"1","citation":{"ieee":"K. Elsayad <i>et al.</i>, “Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging,” <i>Science Signaling</i>, vol. 9, no. 435. American Association for the Advancement of Science, 2016.","apa":"Elsayad, K., Werner, S., Gallemi, M., Kong, J., Guajardo, E., Zhang, L., … Belkhadir, Y. (2016). Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. <i>Science Signaling</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/scisignal.aaf6326\">https://doi.org/10.1126/scisignal.aaf6326</a>","ista":"Elsayad K, Werner S, Gallemi M, Kong J, Guajardo E, Zhang L, Jaillais Y, Greb T, Belkhadir Y. 2016. Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. Science Signaling. 9(435), rs5.","chicago":"Elsayad, Kareem, Stephanie Werner, Marçal Gallemi, Jixiang Kong, Edmundo Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb, and Youssef Belkhadir. “Mapping the Subcellular Mechanical Properties of Live Cells in Tissues with Fluorescence Emission-Brillouin Imaging.” <i>Science Signaling</i>. American Association for the Advancement of Science, 2016. <a href=\"https://doi.org/10.1126/scisignal.aaf6326\">https://doi.org/10.1126/scisignal.aaf6326</a>.","ama":"Elsayad K, Werner S, Gallemi M, et al. Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging. <i>Science Signaling</i>. 2016;9(435). doi:<a href=\"https://doi.org/10.1126/scisignal.aaf6326\">10.1126/scisignal.aaf6326</a>","mla":"Elsayad, Kareem, et al. “Mapping the Subcellular Mechanical Properties of Live Cells in Tissues with Fluorescence Emission-Brillouin Imaging.” <i>Science Signaling</i>, vol. 9, no. 435, rs5, American Association for the Advancement of Science, 2016, doi:<a href=\"https://doi.org/10.1126/scisignal.aaf6326\">10.1126/scisignal.aaf6326</a>.","short":"K. Elsayad, S. Werner, M. Gallemi, J. Kong, E. Guajardo, L. Zhang, Y. Jaillais, T. Greb, Y. Belkhadir, Science Signaling 9 (2016)."},"oa_version":"None","author":[{"first_name":"Kareem","last_name":"Elsayad","full_name":"Elsayad, Kareem"},{"last_name":"Werner","first_name":"Stephanie","full_name":"Werner, Stephanie"},{"first_name":"Marcal","last_name":"Gallemi Rovira","full_name":"Gallemi Rovira, Marcal","id":"460C6802-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Kong","first_name":"Jixiang","full_name":"Kong, Jixiang"},{"last_name":"Guajardo","first_name":"Edmundo","full_name":"Guajardo, Edmundo"},{"first_name":"Lijuan","last_name":"Zhang","full_name":"Zhang, Lijuan"},{"first_name":"Yvon","last_name":"Jaillais","full_name":"Jaillais, Yvon"},{"full_name":"Greb, Thomas","last_name":"Greb","first_name":"Thomas"},{"full_name":"Belkhadir, Youssef","first_name":"Youssef","last_name":"Belkhadir"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"Extracellular matrices (ECMs) are central to the advent of multicellular life, and their mechanical propertiesare modulated by and impinge on intracellular signaling pathways that regulate vital cellular functions. High spatial-resolution mapping of mechanical properties in live cells is, however, extremely challenging. Thus, our understanding of how signaling pathways process physiological signals to generate appropriate mechanical responses is limited. We introduce fluorescence emission-Brillouin scattering imaging (FBi), a method for the parallel and all-optical measurements of mechanical properties and fluorescence at the submicrometer scale in living organisms. Using FBi, we showed thatchanges in cellular hydrostatic pressure and cytoplasm viscoelasticity modulate the mechanical signatures of plant ECMs. We further established that the measured &quot;stiffness&quot; of plant ECMs is symmetrically patternedin hypocotyl cells undergoing directional growth. Finally, application of this method to Arabidopsis thaliana with photoreceptor mutants revealed that red and far-red light signals are essential modulators of ECM viscoelasticity. By mapping the viscoelastic signatures of a complex ECM, we provide proof of principlefor the organism-wide applicability of FBi for measuring the mechanical outputs of intracellular signaling pathways. As such, our work has implications for investigations of mechanosignaling pathways and developmental biology."}],"publication_status":"published","month":"07","issue":"435","article_number":"rs5","language":[{"iso":"eng"}],"date_published":"2016-07-05T00:00:00Z","publisher":"American Association for the Advancement of Science","volume":9,"title":"Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission-Brillouin imaging","date_created":"2018-12-11T11:51:02Z","_id":"1265","type":"journal_article","year":"2016","doi":"10.1126/scisignal.aaf6326","status":"public","day":"05","scopus_import":1,"intvolume":"         9","publist_id":"6057","publication":"Science Signaling"},{"scopus_import":1,"publication":"eLife","intvolume":"         5","publist_id":"6056","oa":1,"day":"01","doi":"10.7554/eLife.13824","year":"2016","acknowledgement":"Boris Gutkin acknowledges funding by the Russian Academic Excellence Project '5-100’.","ddc":["571"],"abstract":[{"text":"Cortical networks exhibit ‘global oscillations’, in which neural spike times are entrained to an underlying oscillatory rhythm, but where individual neurons fire irregularly, on only a fraction of cycles. While the network dynamics underlying global oscillations have been well characterised, their function is debated. Here, we show that such global oscillations are a direct consequence of optimal efficient coding in spiking networks with synaptic delays and noise. To avoid firing unnecessary spikes, neurons need to share information about the network state. Ideally, membrane potentials should be strongly correlated and reflect a ‘prediction error’ while the spikes themselves are uncorrelated and occur rarely. We show that the most efficient representation is when: (i) spike times are entrained to a global Gamma rhythm (implying a consistent representation of the error); but (ii) few neurons fire on each cycle (implying high efficiency), while (iii) excitation and inhibition are tightly balanced. This suggests that cortical networks exhibiting such dynamics are tuned to achieve a maximally efficient population code.","lang":"eng"}],"publication_status":"published","title":"Neural oscillations as a signature of efficient coding in the presence of synaptic delays","publisher":"eLife Sciences Publications","date_published":"2016-07-01T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","citation":{"ieee":"M. J. Chalk, B. Gutkin, and S. Denève, “Neural oscillations as a signature of efficient coding in the presence of synaptic delays,” <i>eLife</i>, vol. 5, no. 2016JULY. eLife Sciences Publications, 2016.","apa":"Chalk, M. J., Gutkin, B., &#38; Denève, S. (2016). Neural oscillations as a signature of efficient coding in the presence of synaptic delays. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.13824\">https://doi.org/10.7554/eLife.13824</a>","ista":"Chalk MJ, Gutkin B, Denève S. 2016. Neural oscillations as a signature of efficient coding in the presence of synaptic delays. eLife. 5(2016JULY), e13824.","chicago":"Chalk, Matthew J, Boris Gutkin, and Sophie Denève. “Neural Oscillations as a Signature of Efficient Coding in the Presence of Synaptic Delays.” <i>ELife</i>. eLife Sciences Publications, 2016. <a href=\"https://doi.org/10.7554/eLife.13824\">https://doi.org/10.7554/eLife.13824</a>.","ama":"Chalk MJ, Gutkin B, Denève S. Neural oscillations as a signature of efficient coding in the presence of synaptic delays. <i>eLife</i>. 2016;5(2016JULY). doi:<a href=\"https://doi.org/10.7554/eLife.13824\">10.7554/eLife.13824</a>","short":"M.J. Chalk, B. Gutkin, S. Denève, ELife 5 (2016).","mla":"Chalk, Matthew J., et al. “Neural Oscillations as a Signature of Efficient Coding in the Presence of Synaptic Delays.” <i>ELife</i>, vol. 5, no. 2016JULY, e13824, eLife Sciences Publications, 2016, doi:<a href=\"https://doi.org/10.7554/eLife.13824\">10.7554/eLife.13824</a>."},"date_updated":"2021-01-12T06:49:30Z","department":[{"_id":"GaTk"}],"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","pubrep_id":"700","_id":"1266","type":"journal_article","date_created":"2018-12-11T11:51:02Z","status":"public","issue":"2016JULY","file":[{"relation":"main_file","access_level":"open_access","file_size":2819055,"file_id":"4874","checksum":"dc52d967dc76174477bb258d84be2899","creator":"system","date_created":"2018-12-12T10:11:20Z","date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf","file_name":"IST-2016-700-v1+1_e13824-download.pdf"}],"article_number":"e13824","has_accepted_license":"1","month":"07","volume":5,"language":[{"iso":"eng"}],"oa_version":"Published Version","quality_controlled":"1","author":[{"first_name":"Matthew J","last_name":"Chalk","orcid":"0000-0001-7782-4436","id":"2BAAC544-F248-11E8-B48F-1D18A9856A87","full_name":"Chalk, Matthew J"},{"first_name":"Boris","last_name":"Gutkin","full_name":"Gutkin, Boris"},{"last_name":"Denève","first_name":"Sophie","full_name":"Denève, Sophie"}]},{"project":[{"call_identifier":"FWF","name":"Structure of the Excitation Spectrum for Many-Body Quantum Systems","grant_number":"P27533_N27","_id":"25C878CE-B435-11E9-9278-68D0E5697425"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"quality_controlled":"1","oa_version":"Published Version","author":[{"first_name":"Rupert","last_name":"Frank","full_name":"Frank, Rupert"},{"first_name":"Rowan","last_name":"Killip","full_name":"Killip, Rowan"},{"first_name":"Phan","last_name":"Nam","id":"404092F4-F248-11E8-B48F-1D18A9856A87","full_name":"Nam, Phan"}],"month":"08","has_accepted_license":"1","issue":"8","file":[{"file_name":"IST-2016-698-v1+1_s11005-016-0860-8.pdf","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:11:09Z","content_type":"application/pdf","file_size":349464,"access_level":"open_access","creator":"system","file_id":"4863","checksum":"d740a6a226e0f5f864f40e3e269d3cc0","relation":"main_file"}],"language":[{"iso":"eng"}],"volume":106,"date_created":"2018-12-11T11:51:02Z","_id":"1267","type":"journal_article","status":"public","pubrep_id":"698","date_updated":"2021-01-12T06:49:30Z","department":[{"_id":"RoSe"}],"citation":{"short":"R. Frank, R. Killip, P. Nam, Letters in Mathematical Physics 106 (2016) 1033–1036.","mla":"Frank, Rupert, et al. “Nonexistence of Large Nuclei in the Liquid Drop Model.” <i>Letters in Mathematical Physics</i>, vol. 106, no. 8, Springer, 2016, pp. 1033–36, doi:<a href=\"https://doi.org/10.1007/s11005-016-0860-8\">10.1007/s11005-016-0860-8</a>.","ama":"Frank R, Killip R, Nam P. Nonexistence of large nuclei in the liquid drop model. <i>Letters in Mathematical Physics</i>. 2016;106(8):1033-1036. doi:<a href=\"https://doi.org/10.1007/s11005-016-0860-8\">10.1007/s11005-016-0860-8</a>","chicago":"Frank, Rupert, Rowan Killip, and Phan Nam. “Nonexistence of Large Nuclei in the Liquid Drop Model.” <i>Letters in Mathematical Physics</i>. Springer, 2016. <a href=\"https://doi.org/10.1007/s11005-016-0860-8\">https://doi.org/10.1007/s11005-016-0860-8</a>.","ista":"Frank R, Killip R, Nam P. 2016. Nonexistence of large nuclei in the liquid drop model. Letters in Mathematical Physics. 106(8), 1033–1036.","apa":"Frank, R., Killip, R., &#38; Nam, P. (2016). Nonexistence of large nuclei in the liquid drop model. <i>Letters in Mathematical Physics</i>. Springer. <a href=\"https://doi.org/10.1007/s11005-016-0860-8\">https://doi.org/10.1007/s11005-016-0860-8</a>","ieee":"R. Frank, R. Killip, and P. Nam, “Nonexistence of large nuclei in the liquid drop model,” <i>Letters in Mathematical Physics</i>, vol. 106, no. 8. Springer, pp. 1033–1036, 2016."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"abstract":[{"lang":"eng","text":"We give a simplified proof of the nonexistence of large nuclei in the liquid drop model and provide an explicit bound. Our bound is within a factor of 2.3 of the conjectured value and seems to be the first quantitative result."}],"publication_status":"published","ddc":["510","539"],"date_published":"2016-08-01T00:00:00Z","publisher":"Springer","file_date_updated":"2020-07-14T12:44:42Z","title":"Nonexistence of large nuclei in the liquid drop model","oa":1,"acknowledgement":"Open access funding provided by Institute of Science and Technology Austria.\r\n","day":"01","doi":"10.1007/s11005-016-0860-8","page":"1033 - 1036","year":"2016","scopus_import":1,"intvolume":"       106","publist_id":"6054","publication":"Letters in Mathematical Physics"},{"issue":"4","month":"08","publication_status":"published","title":"Immune memory in invertebrates","volume":28,"date_published":"2016-08-01T00:00:00Z","publisher":"Academic Press","language":[{"iso":"eng"}],"citation":{"mla":"Milutinovic, Barbara, and Joachim Kurtz. “Immune Memory in Invertebrates.” <i>Seminars in Immunology</i>, vol. 28, no. 4, Academic Press, 2016, pp. 328–42, doi:<a href=\"https://doi.org/10.1016/j.smim.2016.05.004\">10.1016/j.smim.2016.05.004</a>.","short":"B. Milutinovic, J. Kurtz, Seminars in Immunology 28 (2016) 328–342.","chicago":"Milutinovic, Barbara, and Joachim Kurtz. “Immune Memory in Invertebrates.” <i>Seminars in Immunology</i>. Academic Press, 2016. <a href=\"https://doi.org/10.1016/j.smim.2016.05.004\">https://doi.org/10.1016/j.smim.2016.05.004</a>.","ama":"Milutinovic B, Kurtz J. Immune memory in invertebrates. <i>Seminars in Immunology</i>. 2016;28(4):328-342. doi:<a href=\"https://doi.org/10.1016/j.smim.2016.05.004\">10.1016/j.smim.2016.05.004</a>","ista":"Milutinovic B, Kurtz J. 2016. Immune memory in invertebrates. Seminars in Immunology. 28(4), 328–342.","apa":"Milutinovic, B., &#38; Kurtz, J. (2016). Immune memory in invertebrates. <i>Seminars in Immunology</i>. Academic Press. <a href=\"https://doi.org/10.1016/j.smim.2016.05.004\">https://doi.org/10.1016/j.smim.2016.05.004</a>","ieee":"B. Milutinovic and J. Kurtz, “Immune memory in invertebrates,” <i>Seminars in Immunology</i>, vol. 28, no. 4. Academic Press, pp. 328–342, 2016."},"oa_version":"None","quality_controlled":"1","department":[{"_id":"SyCr"}],"date_updated":"2021-01-12T06:49:30Z","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","author":[{"orcid":"0000-0002-8214-4758","last_name":"Milutinovic","first_name":"Barbara","full_name":"Milutinovic, Barbara","id":"2CDC32B8-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Joachim","last_name":"Kurtz","full_name":"Kurtz, Joachim"}],"scopus_import":1,"publication":"Seminars in Immunology","publist_id":"6053","intvolume":"        28","type":"journal_article","_id":"1268","date_created":"2018-12-11T11:51:03Z","day":"01","page":"328 - 342","doi":"10.1016/j.smim.2016.05.004","year":"2016","status":"public","acknowledgement":"We would like to thank Mihai Netea for inviting us to contribute to this Theme Issue."},{"author":[{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739"}],"quality_controlled":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"volume":91,"has_accepted_license":"1","month":"08","file":[{"date_created":"2018-12-12T10:18:28Z","date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf","file_name":"IST-2016-697-v1+1_s11103-016-0501-8.pdf","relation":"main_file","access_level":"open_access","file_size":297282,"file_id":"5349","checksum":"0ffb7a15c5336b3a55248cc67021a825","creator":"system"}],"issue":"6","status":"public","date_created":"2018-12-11T11:51:03Z","_id":"1269","type":"journal_article","pubrep_id":"697","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"EvBe"}],"date_updated":"2021-01-12T06:49:31Z","citation":{"apa":"Benková, E. (2016). Plant hormones in interactions with the environment. <i>Plant Molecular Biology</i>. Springer. <a href=\"https://doi.org/10.1007/s11103-016-0501-8\">https://doi.org/10.1007/s11103-016-0501-8</a>","ama":"Benková E. Plant hormones in interactions with the environment. <i>Plant Molecular Biology</i>. 2016;91(6):597. doi:<a href=\"https://doi.org/10.1007/s11103-016-0501-8\">10.1007/s11103-016-0501-8</a>","chicago":"Benková, Eva. “Plant Hormones in Interactions with the Environment.” <i>Plant Molecular Biology</i>. Springer, 2016. <a href=\"https://doi.org/10.1007/s11103-016-0501-8\">https://doi.org/10.1007/s11103-016-0501-8</a>.","ista":"Benková E. 2016. Plant hormones in interactions with the environment. Plant Molecular Biology. 91(6), 597.","ieee":"E. Benková, “Plant hormones in interactions with the environment,” <i>Plant Molecular Biology</i>, vol. 91, no. 6. Springer, p. 597, 2016.","mla":"Benková, Eva. “Plant Hormones in Interactions with the Environment.” <i>Plant Molecular Biology</i>, vol. 91, no. 6, Springer, 2016, p. 597, doi:<a href=\"https://doi.org/10.1007/s11103-016-0501-8\">10.1007/s11103-016-0501-8</a>.","short":"E. Benková, Plant Molecular Biology 91 (2016) 597."},"date_published":"2016-08-01T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","publisher":"Springer","title":"Plant hormones in interactions with the environment","publication_status":"published","abstract":[{"text":"Plants are continuously exposed to a myriad of external signals such as fluctuating nutrients availability, drought, heat, cold, high salinity, or pathogen/pest attacks that can severely affect their development, growth, and fertility. As sessile organisms, plants must therefore be able to sense and rapidly react to these external inputs, activate efficient responses, and adjust development to changing conditions. In recent years, significant progress has been made towards understanding the molecular mechanisms underlying the intricate and complex communication between plants and the environment. It is now becoming increasingly evident that hormones have an important regulatory role in plant adaptation and defense mechanisms.","lang":"eng"}],"ddc":["581"],"year":"2016","page":"597","doi":"10.1007/s11103-016-0501-8","day":"01","oa":1,"publist_id":"6052","intvolume":"        91","publication":"Plant Molecular Biology","scopus_import":1},{"status":"public","_id":"1270","type":"journal_article","date_created":"2018-12-11T11:51:03Z","pubrep_id":"696","author":[{"full_name":"Hillenbrand, Patrick","first_name":"Patrick","last_name":"Hillenbrand"},{"first_name":"Ulrich","last_name":"Gerland","full_name":"Gerland, Ulrich"},{"last_name":"Tkacik","first_name":"Gasper","orcid":"0000-0002-6699-1455","id":"3D494DCA-F248-11E8-B48F-1D18A9856A87","full_name":"Tkacik, Gasper"}],"oa_version":"Published Version","project":[{"call_identifier":"FWF","name":"Biophysics of information processing in gene regulation","_id":"254E9036-B435-11E9-9278-68D0E5697425","grant_number":"P28844-B27"}],"quality_controlled":"1","volume":11,"language":[{"iso":"eng"}],"issue":"9","file":[{"file_name":"IST-2016-696-v1+1_journal.pone.0163628.PDF","date_created":"2018-12-12T10:10:47Z","date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf","access_level":"open_access","file_size":4950415,"file_id":"4837","checksum":"3d0d55d373096a033bd9cf79288c8586","creator":"system","relation":"main_file"}],"article_number":"e0163628","has_accepted_license":"1","month":"09","year":"2016","day":"27","doi":"10.1371/journal.pone.0163628","acknowledgement":"The authors would like to thank Thomas Sokolowski and Filipe Tostevin for helpful discussions. PH and UG were funded by the German Excellence Initiative via the program \"Nanosystems Initiative Munich\" (https://www.nano-initiative-munich.de) and the German Research Foundation via the SFB 1032 \"Nanoagents for Spatiotemporal Control of Molecular and Cellular Reactions\" (http://www.sfb1032.physik.uni-muenchen.de). GT was funded by the Austrian Science Fund (FWF P 28844) (http://www.fwf.ac.at).","oa":1,"publication":"PLoS One","intvolume":"        11","publist_id":"6050","related_material":{"record":[{"relation":"research_data","id":"9869","status":"public"},{"relation":"research_data","id":"9870","status":"public"},{"status":"public","relation":"research_data","id":"9871"}]},"scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"citation":{"mla":"Hillenbrand, Patrick, et al. “Beyond the French Flag Model: Exploiting Spatial and Gene Regulatory Interactions for Positional Information.” <i>PLoS One</i>, vol. 11, no. 9, e0163628, Public Library of Science, 2016, doi:<a href=\"https://doi.org/10.1371/journal.pone.0163628\">10.1371/journal.pone.0163628</a>.","short":"P. Hillenbrand, U. Gerland, G. Tkačik, PLoS One 11 (2016).","ieee":"P. Hillenbrand, U. Gerland, and G. Tkačik, “Beyond the French flag model: Exploiting spatial and gene regulatory interactions for positional information,” <i>PLoS One</i>, vol. 11, no. 9. Public Library of Science, 2016.","ista":"Hillenbrand P, Gerland U, Tkačik G. 2016. Beyond the French flag model: Exploiting spatial and gene regulatory interactions for positional information. PLoS One. 11(9), e0163628.","ama":"Hillenbrand P, Gerland U, Tkačik G. Beyond the French flag model: Exploiting spatial and gene regulatory interactions for positional information. <i>PLoS One</i>. 2016;11(9). doi:<a href=\"https://doi.org/10.1371/journal.pone.0163628\">10.1371/journal.pone.0163628</a>","chicago":"Hillenbrand, Patrick, Ulrich Gerland, and Gašper Tkačik. “Beyond the French Flag Model: Exploiting Spatial and Gene Regulatory Interactions for Positional Information.” <i>PLoS One</i>. Public Library of Science, 2016. <a href=\"https://doi.org/10.1371/journal.pone.0163628\">https://doi.org/10.1371/journal.pone.0163628</a>.","apa":"Hillenbrand, P., Gerland, U., &#38; Tkačik, G. (2016). Beyond the French flag model: Exploiting spatial and gene regulatory interactions for positional information. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0163628\">https://doi.org/10.1371/journal.pone.0163628</a>"},"date_updated":"2023-02-23T14:11:37Z","department":[{"_id":"GaTk"}],"title":"Beyond the French flag model: Exploiting spatial and gene regulatory interactions for positional information","file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-09-27T00:00:00Z","publisher":"Public Library of Science","ddc":["571"],"abstract":[{"lang":"eng","text":"A crucial step in the early development of multicellular organisms involves the establishment of spatial patterns of gene expression which later direct proliferating cells to take on different cell fates. These patterns enable the cells to infer their global position within a tissue or an organism by reading out local gene expression levels. The patterning system is thus said to encode positional information, a concept that was formalized recently in the framework of information theory. Here we introduce a toy model of patterning in one spatial dimension, which can be seen as an extension of Wolpert's paradigmatic &quot;French Flag&quot; model, to patterning by several interacting, spatially coupled genes subject to intrinsic and extrinsic noise. Our model, a variant of an Ising spin system, allows us to systematically explore expression patterns that optimally encode positional information. We find that optimal patterning systems use positional cues, as in the French Flag model, together with gene-gene interactions to generate combinatorial codes for position which we call &quot;Counter&quot; patterns. Counter patterns can also be stabilized against noise and variations in system size or morphogen dosage by longer-range spatial interactions of the type invoked in the Turing model. The simple setup proposed here qualitatively captures many of the experimentally observed properties of biological patterning systems and allows them to be studied in a single, theoretically consistent framework."}],"publication_status":"published"},{"acknowledgement":"We thank K. Lee, C. Norden, A. Webb, and the members of the Paluch lab for\r\ncomments on the manuscript. We are grateful to P. Rørth and Peter Dieterich\r\nfor discussions, S. Ares, Y. Arboleda-Estudillo and S. Schneider for technical help,\r\nM. Biro for help with programming, and the BIOTEC/MPI-CBG and IST zebrafish\r\nand imaging facilities for help and advice at various stages of this project. This work was supported by the Max Planck Society, the Medical Research Council UK (core funding to the MRC LMCB), and by grants from the Polish Ministry of Science and Higher Education (454/N-MPG/2009/0) to EKP, the Deutsche Forschungsgemeinschaft (HE 3231/6-1 and PA 1590/1-1) to CPH and EKP, a A*Star JCO career development award (12302FG010) to WY and a Damon Runyon fellowship award to ADM (DRG 2157-12). This work was also supported by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001317), the UK Medical Research Council (FC001317), and the Wellcome Trust (FC001317) to GS.","doi":"10.1186/s12915-016-0294-x","day":"02","year":"2016","oa":1,"intvolume":"        14","publist_id":"6049","publication":"BMC Biology","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_updated":"2021-01-12T06:49:32Z","department":[{"_id":"CaHe"}],"citation":{"ista":"Diz Muñoz A, Romanczuk P, Yu W, Bergert M, Ivanovitch K, Salbreux G, Heisenberg C-PJ, Paluch E. 2016. Steering cell migration by alternating blebs and actin-rich protrusions. BMC Biology. 14(1), 74.","chicago":"Diz Muñoz, Alba, Pawel Romanczuk, Weimiao Yu, Martin Bergert, Kenzo Ivanovitch, Guillame Salbreux, Carl-Philipp J Heisenberg, and Ewa Paluch. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” <i>BMC Biology</i>. BioMed Central, 2016. <a href=\"https://doi.org/10.1186/s12915-016-0294-x\">https://doi.org/10.1186/s12915-016-0294-x</a>.","ama":"Diz Muñoz A, Romanczuk P, Yu W, et al. Steering cell migration by alternating blebs and actin-rich protrusions. <i>BMC Biology</i>. 2016;14(1). doi:<a href=\"https://doi.org/10.1186/s12915-016-0294-x\">10.1186/s12915-016-0294-x</a>","apa":"Diz Muñoz, A., Romanczuk, P., Yu, W., Bergert, M., Ivanovitch, K., Salbreux, G., … Paluch, E. (2016). Steering cell migration by alternating blebs and actin-rich protrusions. <i>BMC Biology</i>. BioMed Central. <a href=\"https://doi.org/10.1186/s12915-016-0294-x\">https://doi.org/10.1186/s12915-016-0294-x</a>","ieee":"A. Diz Muñoz <i>et al.</i>, “Steering cell migration by alternating blebs and actin-rich protrusions,” <i>BMC Biology</i>, vol. 14, no. 1. BioMed Central, 2016.","mla":"Diz Muñoz, Alba, et al. “Steering Cell Migration by Alternating Blebs and Actin-Rich Protrusions.” <i>BMC Biology</i>, vol. 14, no. 1, 74, BioMed Central, 2016, doi:<a href=\"https://doi.org/10.1186/s12915-016-0294-x\">10.1186/s12915-016-0294-x</a>.","short":"A. Diz Muñoz, P. Romanczuk, W. Yu, M. Bergert, K. Ivanovitch, G. Salbreux, C.-P.J. Heisenberg, E. Paluch, BMC Biology 14 (2016)."},"date_published":"2016-09-02T00:00:00Z","publisher":"BioMed Central","file_date_updated":"2020-07-14T12:44:42Z","title":"Steering cell migration by alternating blebs and actin-rich protrusions","abstract":[{"text":"Background: High directional persistence is often assumed to enhance the efficiency of chemotactic migration. Yet, cells in vivo usually display meandering trajectories with relatively low directional persistence, and the control and function of directional persistence during cell migration in three-dimensional environments are poorly understood. Results: Here, we use mesendoderm progenitors migrating during zebrafish gastrulation as a model system to investigate the control of directional persistence during migration in vivo. We show that progenitor cells alternate persistent run phases with tumble phases that result in cell reorientation. Runs are characterized by the formation of directed actin-rich protrusions and tumbles by enhanced blebbing. Increasing the proportion of actin-rich protrusions or blebs leads to longer or shorter run phases, respectively. Importantly, both reducing and increasing run phases result in larger spatial dispersion of the cells, indicative of reduced migration precision. A physical model quantitatively recapitulating the migratory behavior of mesendoderm progenitors indicates that the ratio of tumbling to run times, and thus the specific degree of directional persistence of migration, are critical for optimizing migration precision. Conclusions: Together, our experiments and model provide mechanistic insight into the control of migration directionality for cells moving in three-dimensional environments that combine different protrusion types, whereby the proportion of blebs to actin-rich protrusions determines the directional persistence and precision of movement by regulating the ratio of tumbling to run times.","lang":"eng"}],"publication_status":"published","ddc":["572","576"],"status":"public","date_created":"2018-12-11T11:51:04Z","_id":"1271","type":"journal_article","pubrep_id":"695","author":[{"full_name":"Diz Muñoz, Alba","last_name":"Diz Muñoz","first_name":"Alba"},{"full_name":"Romanczuk, Pawel","last_name":"Romanczuk","first_name":"Pawel"},{"full_name":"Yu, Weimiao","last_name":"Yu","first_name":"Weimiao"},{"full_name":"Bergert, Martin","first_name":"Martin","last_name":"Bergert"},{"first_name":"Kenzo","last_name":"Ivanovitch","full_name":"Ivanovitch, Kenzo"},{"first_name":"Guillame","last_name":"Salbreux","full_name":"Salbreux, Guillame"},{"orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ewa","last_name":"Paluch","full_name":"Paluch, Ewa"}],"quality_controlled":"1","project":[{"name":"Analysis of the Formation and Function of Different Cell Protusion Types During Cell Migration in Vivo","_id":"252064B8-B435-11E9-9278-68D0E5697425","grant_number":"HE_3231/6-1"}],"oa_version":"Published Version","language":[{"iso":"eng"}],"acknowledged_ssus":[{"_id":"LifeSc"}],"volume":14,"has_accepted_license":"1","month":"09","issue":"1","article_number":"74","file":[{"file_name":"IST-2016-695-v1+1_s12915-016-0294-x.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:13:20Z","date_updated":"2020-07-14T12:44:42Z","file_id":"5002","checksum":"0bfa484ac69a0a560fb9a4589aeda7f6","creator":"system","access_level":"open_access","file_size":1875695,"relation":"main_file"}]},{"oa":1,"acknowledgement":"This work was supported by Austrian Science Fund (FWF): P25816-N15.","page":"712 - 721","year":"2016","doi":"10.1080/16864360.2016.1150718","day":"02","scopus_import":1,"intvolume":"        13","publist_id":"6048","publication":"Computer-Aided Design and Applications","date_updated":"2021-01-12T06:49:32Z","department":[{"_id":"HeEd"}],"citation":{"ista":"Held M, Huber S, Palfrader P. 2016. Generalized offsetting of planar structures using skeletons. Computer-Aided Design and Applications. 13(5), 712–721.","chicago":"Held, Martin, Stefan Huber, and Peter Palfrader. “Generalized Offsetting of Planar Structures Using Skeletons.” <i>Computer-Aided Design and Applications</i>. Taylor and Francis, 2016. <a href=\"https://doi.org/10.1080/16864360.2016.1150718\">https://doi.org/10.1080/16864360.2016.1150718</a>.","ama":"Held M, Huber S, Palfrader P. Generalized offsetting of planar structures using skeletons. <i>Computer-Aided Design and Applications</i>. 2016;13(5):712-721. doi:<a href=\"https://doi.org/10.1080/16864360.2016.1150718\">10.1080/16864360.2016.1150718</a>","apa":"Held, M., Huber, S., &#38; Palfrader, P. (2016). Generalized offsetting of planar structures using skeletons. <i>Computer-Aided Design and Applications</i>. Taylor and Francis. <a href=\"https://doi.org/10.1080/16864360.2016.1150718\">https://doi.org/10.1080/16864360.2016.1150718</a>","ieee":"M. Held, S. Huber, and P. Palfrader, “Generalized offsetting of planar structures using skeletons,” <i>Computer-Aided Design and Applications</i>, vol. 13, no. 5. Taylor and Francis, pp. 712–721, 2016.","short":"M. Held, S. Huber, P. Palfrader, Computer-Aided Design and Applications 13 (2016) 712–721.","mla":"Held, Martin, et al. “Generalized Offsetting of Planar Structures Using Skeletons.” <i>Computer-Aided Design and Applications</i>, vol. 13, no. 5, Taylor and Francis, 2016, pp. 712–21, doi:<a href=\"https://doi.org/10.1080/16864360.2016.1150718\">10.1080/16864360.2016.1150718</a>."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"We study different means to extend offsetting based on skeletal structures beyond the well-known constant-radius and mitered offsets supported by Voronoi diagrams and straight skeletons, for which the orthogonal distance of offset elements to their respective input elements is constant and uniform over all input elements. Our main contribution is a new geometric structure, called variable-radius Voronoi diagram, which supports the computation of variable-radius offsets, i.e., offsets whose distance to the input is allowed to vary along the input. We discuss properties of this structure and sketch a prototype implementation that supports the computation of variable-radius offsets based on this new variant of Voronoi diagrams."}],"publication_status":"published","ddc":["004","516"],"publisher":"Taylor and Francis","file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-09-02T00:00:00Z","title":"Generalized offsetting of planar structures using skeletons","date_created":"2018-12-11T11:51:04Z","type":"journal_article","_id":"1272","status":"public","license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","pubrep_id":"694","quality_controlled":"1","oa_version":"Published Version","author":[{"first_name":"Martin","last_name":"Held","full_name":"Held, Martin"},{"orcid":"0000-0002-8871-5814","last_name":"Huber","first_name":"Stefan","full_name":"Huber, Stefan","id":"4700A070-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Peter","last_name":"Palfrader","full_name":"Palfrader, Peter"}],"has_accepted_license":"1","month":"09","issue":"5","file":[{"date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:16:20Z","content_type":"application/pdf","file_name":"IST-2016-694-v1+1_Generalized_offsetting_of_planar_structures_using_skeletons.pdf","relation":"main_file","file_size":1678369,"access_level":"open_access","creator":"system","checksum":"c746f3a48edb62b588d92ea5d0fd2c0e","file_id":"5206"}],"language":[{"iso":"eng"}],"volume":13},{"day":"13","year":"2016","page":"3340 - 3349","doi":"10.1242/dev.136283","acknowledgement":"We acknowledge the support of glasshouse technicians at the University of\r\nNottingham for help with plant growth and the Nottingham\r\nArabidopsis\r\nStock Centre\r\n(NASC) for providing\r\nArabidopsis\r\nlines. This research was supported by the Biotechnology and Biological Sciences Research Council (BBSRC) (to A.B. and M.J.B.); the European Research Council (ERC) Advanced Grant SysArc (to B.S.) and FUTUREROOTS (to M.J.B.); The Royal Society for University and Wolfson Research Fellowship awards (to A.B. and M.J.B.); a Federation of European Biochemical Societies (FEBS) Long-Term Fellowship (to B.P.); an Intra-European Fellowship for Career Development under the 7th framework of the European Commission [IEF-2008-220506 to B.P.]; a European Molecular Biology Organization (EMBO) Long-Term Fellowship (to B.P.); and a European Reintegration Grant under the 7th framework of the European Commission [ERG-2010-276662 to B.P.]; Interuniversity Attraction Poles Programme [initiated by the Belgian Science Policy Office (Federaal Wetenschapsbeleid)] (to M.J.B.); The Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan: Grants-in-Aid for Scientific Research on Innovative Areas [25110330 to H.F.] and a JSPS Research Fellowship for Young Scientists [12J02079 to T.G.]; funds for research performed by S.M.B. and A.G. were provided by University of California, Davis startup funds.","oa":1,"publication":"Development","publist_id":"6044","intvolume":"       143","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","citation":{"ama":"Porco S, Larrieu A, Du Y, et al. Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. <i>Development</i>. 2016;143(18):3340-3349. doi:<a href=\"https://doi.org/10.1242/dev.136283\">10.1242/dev.136283</a>","ista":"Porco S, Larrieu A, Du Y, Gaudinier A, Goh T, Swarup K, Swarup R, Kuempers B, Bishopp A, Lavenus J, Casimiro I, Hill K, Benková E, Fukaki H, Brady S, Scheres B, Peéet B, Bennett M. 2016. Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. Development. 143(18), 3340–3349.","chicago":"Porco, Silvana, Antoine Larrieu, Yujuan Du, Allison Gaudinier, Tatsuaki Goh, Kamal Swarup, Ranjan Swarup, et al. “Lateral Root Emergence in Arabidopsis Is Dependent on Transcription Factor LBD29 Regulation of Auxin Influx Carrier LAX3.” <i>Development</i>. Company of Biologists, 2016. <a href=\"https://doi.org/10.1242/dev.136283\">https://doi.org/10.1242/dev.136283</a>.","apa":"Porco, S., Larrieu, A., Du, Y., Gaudinier, A., Goh, T., Swarup, K., … Bennett, M. (2016). Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3. <i>Development</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/dev.136283\">https://doi.org/10.1242/dev.136283</a>","ieee":"S. Porco <i>et al.</i>, “Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3,” <i>Development</i>, vol. 143, no. 18. Company of Biologists, pp. 3340–3349, 2016.","short":"S. Porco, A. Larrieu, Y. Du, A. Gaudinier, T. Goh, K. Swarup, R. Swarup, B. Kuempers, A. Bishopp, J. Lavenus, I. Casimiro, K. Hill, E. Benková, H. Fukaki, S. Brady, B. Scheres, B. Peéet, M. Bennett, Development 143 (2016) 3340–3349.","mla":"Porco, Silvana, et al. “Lateral Root Emergence in Arabidopsis Is Dependent on Transcription Factor LBD29 Regulation of Auxin Influx Carrier LAX3.” <i>Development</i>, vol. 143, no. 18, Company of Biologists, 2016, pp. 3340–49, doi:<a href=\"https://doi.org/10.1242/dev.136283\">10.1242/dev.136283</a>."},"department":[{"_id":"EvBe"}],"date_updated":"2021-01-12T06:49:32Z","title":"Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulation of auxin influx carrier LAX3","publisher":"Company of Biologists","date_published":"2016-09-13T00:00:00Z","publication_status":"published","abstract":[{"lang":"eng","text":"Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin-inducible auxin influx carrier LAX3 plays a key role concentrating this signal in cells overlying LRP. Delimiting LAX3 expression to two adjacent cell files overlying new LRP is crucial to ensure that auxin-regulated cell separation occurs solely along their shared walls. Multiscale modeling has predicted that this highly focused pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN3 and LAX3, respectively. Consistent with model predictions, we report that auxin-inducible LAX3 expression is regulated indirectly by AUXIN RESPONSE FACTOR 7 (ARF7). Yeast one-hybrid screens revealed that the LAX3 promoter is bound by the transcription factor LBD29, which is a direct target for regulation by ARF7. Disrupting auxin-inducible LBD29 expression or expressing an LBD29-SRDX transcriptional repressor phenocopied the lax3 mutant, resulting in delayed lateral root emergence. We conclude that sequential LBD29 and LAX3 induction by auxin is required to coordinate cell separation and organ emergence."}],"status":"public","main_file_link":[{"url":"https://hal.archives-ouvertes.fr/hal-01595056/","open_access":"1"}],"_id":"1273","type":"journal_article","date_created":"2018-12-11T11:51:04Z","author":[{"last_name":"Porco","first_name":"Silvana","full_name":"Porco, Silvana"},{"first_name":"Antoine","last_name":"Larrieu","full_name":"Larrieu, Antoine"},{"first_name":"Yujuan","last_name":"Du","full_name":"Du, Yujuan"},{"last_name":"Gaudinier","first_name":"Allison","full_name":"Gaudinier, Allison"},{"full_name":"Goh, Tatsuaki","last_name":"Goh","first_name":"Tatsuaki"},{"full_name":"Swarup, Kamal","first_name":"Kamal","last_name":"Swarup"},{"full_name":"Swarup, Ranjan","last_name":"Swarup","first_name":"Ranjan"},{"full_name":"Kuempers, Britta","first_name":"Britta","last_name":"Kuempers"},{"full_name":"Bishopp, Anthony","last_name":"Bishopp","first_name":"Anthony"},{"last_name":"Lavenus","first_name":"Julien","full_name":"Lavenus, Julien"},{"last_name":"Casimiro","first_name":"Ilda","full_name":"Casimiro, Ilda"},{"last_name":"Hill","first_name":"Kristine","full_name":"Hill, Kristine"},{"first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"},{"first_name":"Hidehiro","last_name":"Fukaki","full_name":"Fukaki, Hidehiro"},{"full_name":"Brady, Siobhan","first_name":"Siobhan","last_name":"Brady"},{"full_name":"Scheres, Ben","first_name":"Ben","last_name":"Scheres"},{"full_name":"Peéet, Benjamin","first_name":"Benjamin","last_name":"Peéet"},{"last_name":"Bennett","first_name":"Malcolm","full_name":"Bennett, Malcolm"}],"oa_version":"Preprint","quality_controlled":"1","volume":143,"language":[{"iso":"eng"}],"issue":"18","month":"09"},{"pubrep_id":"692","status":"public","date_created":"2018-12-11T11:51:05Z","type":"journal_article","_id":"1274","language":[{"iso":"eng"}],"volume":6,"article_processing_charge":"No","month":"09","has_accepted_license":"1","article_number":"33754","file":[{"relation":"main_file","file_id":"5008","checksum":"ee371fbc9124ad93157a95829264e4fe","creator":"system","access_level":"open_access","file_size":2895147,"content_type":"application/pdf","date_created":"2018-12-12T10:13:25Z","date_updated":"2020-07-14T12:44:42Z","file_name":"IST-2016-692-v1+1_srep33754.pdf"}],"author":[{"full_name":"Mazur, Ewa","first_name":"Ewa","last_name":"Mazur"},{"last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596"}],"quality_controlled":"1","oa_version":"Published Version","publist_id":"6042","intvolume":"         6","publication":"Scientific Reports","scopus_import":"1","related_material":{"record":[{"status":"public","relation":"later_version","id":"545"}]},"acknowledgement":"We wish to thank Prof. Ewa U. Kurczyńska for initiation of this work and valuable advices. We thank Martine De Cock for help in preparing the manuscript. This work was supported by the European Research Council (project ERC-2011-StG-20101109-PSDP), the European Social Fund (CZ.1.07/2.3.00/20.0043), and the Czech Science Foundation GAČR (GA13-40637 S) to J.F., (GA 13-39982S) to E.B. and E.M. and in part by the European Regional Development Fund (project “CEITEC, Central European Institute of Technology”, CZ.1.05/1.1.00/02.0068).","doi":"10.1038/srep33754","day":"21","year":"2016","oa":1,"pmid":1,"file_date_updated":"2020-07-14T12:44:42Z","date_published":"2016-09-21T00:00:00Z","publisher":"Nature Publishing Group","title":"Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis","publication_status":"published","abstract":[{"lang":"eng","text":"Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis."}],"ddc":["581"],"external_id":{"pmid":["27649687"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"EvBe"},{"_id":"JiFr"}],"date_updated":"2025-05-07T11:12:28Z","citation":{"ista":"Mazur E, Benková E, Friml J. 2016. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific Reports. 6, 33754.","chicago":"Mazur, Ewa, Eva Benková, and Jiří Friml. “Vascular Cambium Regeneration and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep33754\">https://doi.org/10.1038/srep33754</a>.","ama":"Mazur E, Benková E, Friml J. Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep33754\">10.1038/srep33754</a>","apa":"Mazur, E., Benková, E., &#38; Friml, J. (2016). Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep33754\">https://doi.org/10.1038/srep33754</a>","ieee":"E. Mazur, E. Benková, and J. Friml, “Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016.","short":"E. Mazur, E. Benková, J. Friml, Scientific Reports 6 (2016).","mla":"Mazur, Ewa, et al. “Vascular Cambium Regeneration and Vessel Formation in Wounded Inflorescence Stems of Arabidopsis.” <i>Scientific Reports</i>, vol. 6, 33754, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep33754\">10.1038/srep33754</a>."}},{"publication":"Physical Review Letters","publist_id":"6041","intvolume":"       117","scopus_import":1,"status":"public","doi":"10.1103/PhysRevLett.117.139802","day":"22","year":"2016","_id":"1275","type":"journal_article","date_created":"2018-12-11T11:51:05Z","title":"Callan-Jones et al. Reply","volume":117,"date_published":"2016-09-22T00:00:00Z","language":[{"iso":"eng"}],"publisher":"American Physical Society","article_number":"139802","issue":"13","month":"09","publication_status":"published","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","author":[{"last_name":"Callan Jones","first_name":"Andrew","full_name":"Callan Jones, Andrew"},{"full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","last_name":"Ruprecht","first_name":"Verena"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","full_name":"Wieser, Stefan","first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566"},{"last_name":"Voituriez","first_name":"Raphaël","full_name":"Voituriez, Raphaël"}],"citation":{"short":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P.J. Heisenberg, R. Voituriez, Physical Review Letters 117 (2016).","mla":"Callan Jones, Andrew, et al. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>, vol. 117, no. 13, 139802, American Physical Society, 2016, doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>.","apa":"Callan Jones, A., Ruprecht, V., Wieser, S., Heisenberg, C.-P. J., &#38; Voituriez, R. (2016). Callan-Jones et al. Reply. <i>Physical Review Letters</i>. American Physical Society. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>","ista":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. 2016. Callan-Jones et al. Reply. Physical Review Letters. 117(13), 139802.","ama":"Callan Jones A, Ruprecht V, Wieser S, Heisenberg C-PJ, Voituriez R. Callan-Jones et al. Reply. <i>Physical Review Letters</i>. 2016;117(13). doi:<a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">10.1103/PhysRevLett.117.139802</a>","chicago":"Callan Jones, Andrew, Verena Ruprecht, Stefan Wieser, Carl-Philipp J Heisenberg, and Raphaël Voituriez. “Callan-Jones et Al. Reply.” <i>Physical Review Letters</i>. American Physical Society, 2016. <a href=\"https://doi.org/10.1103/PhysRevLett.117.139802\">https://doi.org/10.1103/PhysRevLett.117.139802</a>.","ieee":"A. Callan Jones, V. Ruprecht, S. Wieser, C.-P. J. Heisenberg, and R. Voituriez, “Callan-Jones et al. Reply,” <i>Physical Review Letters</i>, vol. 117, no. 13. American Physical Society, 2016."},"oa_version":"None","department":[{"_id":"CaHe"}],"quality_controlled":"1","date_updated":"2021-01-12T06:49:33Z"},{"scopus_import":1,"intvolume":"         6","publist_id":"6040","publication":"Scientific Reports","oa":1,"acknowledgement":"We wish to thank CSC – IT Centre for Science (Espoo, Finland) for computational resources. For financial support, we wish to thank the Academy of Finland (TR, IV and PAP; Center of Excellence in Biomembrane Research (IV, TR)), the Finnish Doctoral Programme in Computational Sciences (KK), the Sigrid Juselius Foundation (IV), the Paulo Foundation (PAP), and the European Research Council (IV, TR; Advanced Grant project CROWDED-PRO-LIPIDS). AO acknowledges The Wellcome Trust International Senior Research Fellowship.","doi":"10.1038/srep33607","year":"2016","day":"26","abstract":[{"lang":"eng","text":"The cytochrome (cyt) bc 1 complex is an integral component of the respiratory electron transfer chain sustaining the energy needs of organisms ranging from humans to bacteria. Due to its ubiquitous role in the energy metabolism, both the oxidation and reduction of the enzyme's substrate co-enzyme Q has been studied vigorously. Here, this vast amount of data is reassessed after probing the substrate reduction steps at the Q i-site of the cyt bc 1 complex of Rhodobacter capsulatus using atomistic molecular dynamics simulations. The simulations suggest that the Lys251 side chain could rotate into the Q i-site to facilitate binding of half-protonated semiquinone-a reaction intermediate that is potentially formed during substrate reduction. At this bent pose, the Lys251 forms a salt bridge with the Asp252, thus making direct proton transfer possible. In the neutral state, the lysine side chain stays close to the conserved binding location of cardiolipin (CL). This back-and-forth motion between the CL and Asp252 indicates that Lys251 functions as a proton shuttle controlled by pH-dependent negative feedback. The CL/K/D switching, which represents a refinement to the previously described CL/K pathway, fine-tunes the proton transfer process. Lastly, the simulation data was used to formulate a mechanism for reducing the substrate at the Q i-site."}],"publication_status":"published","ddc":["576"],"date_published":"2016-09-26T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","publisher":"Nature Publishing Group","title":"Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex","date_updated":"2021-01-12T06:49:34Z","department":[{"_id":"LeSa"}],"citation":{"mla":"Postila, Pekka, et al. “Atomistic Determinants of Co-Enzyme Q Reduction at the Qi-Site of the Cytochrome Bc1 Complex.” <i>Scientific Reports</i>, vol. 6, 33607, Nature Publishing Group, 2016, doi:<a href=\"https://doi.org/10.1038/srep33607\">10.1038/srep33607</a>.","short":"P. Postila, K. Kaszuba, P. Kuleta, I. Vattulainen, M. Sarewicz, A. Osyczka, T. Róg, Scientific Reports 6 (2016).","chicago":"Postila, Pekka, Karol Kaszuba, Patryk Kuleta, Ilpo Vattulainen, Marcin Sarewicz, Artur Osyczka, and Tomasz Róg. “Atomistic Determinants of Co-Enzyme Q Reduction at the Qi-Site of the Cytochrome Bc1 Complex.” <i>Scientific Reports</i>. Nature Publishing Group, 2016. <a href=\"https://doi.org/10.1038/srep33607\">https://doi.org/10.1038/srep33607</a>.","ama":"Postila P, Kaszuba K, Kuleta P, et al. Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex. <i>Scientific Reports</i>. 2016;6. doi:<a href=\"https://doi.org/10.1038/srep33607\">10.1038/srep33607</a>","ista":"Postila P, Kaszuba K, Kuleta P, Vattulainen I, Sarewicz M, Osyczka A, Róg T. 2016. Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex. Scientific Reports. 6, 33607.","apa":"Postila, P., Kaszuba, K., Kuleta, P., Vattulainen, I., Sarewicz, M., Osyczka, A., &#38; Róg, T. (2016). Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex. <i>Scientific Reports</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/srep33607\">https://doi.org/10.1038/srep33607</a>","ieee":"P. Postila <i>et al.</i>, “Atomistic determinants of co-enzyme Q reduction at the Qi-site of the cytochrome bc1 complex,” <i>Scientific Reports</i>, vol. 6. Nature Publishing Group, 2016."},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","pubrep_id":"691","date_created":"2018-12-11T11:51:05Z","type":"journal_article","_id":"1276","status":"public","month":"09","has_accepted_license":"1","article_number":"33607","file":[{"file_name":"IST-2016-691-v1+1_srep33607.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:17:09Z","date_updated":"2020-07-14T12:44:42Z","checksum":"07c591c1250ebef266333cbc3228b4dd","file_id":"5261","creator":"system","access_level":"open_access","file_size":1960563,"relation":"main_file"}],"language":[{"iso":"eng"}],"volume":6,"quality_controlled":"1","oa_version":"Published Version","author":[{"full_name":"Postila, Pekka","last_name":"Postila","first_name":"Pekka"},{"last_name":"Kaszuba","first_name":"Karol","id":"3FDF9472-F248-11E8-B48F-1D18A9856A87","full_name":"Kaszuba, Karol"},{"first_name":"Patryk","last_name":"Kuleta","full_name":"Kuleta, Patryk"},{"full_name":"Vattulainen, Ilpo","last_name":"Vattulainen","first_name":"Ilpo"},{"full_name":"Sarewicz, Marcin","first_name":"Marcin","last_name":"Sarewicz"},{"first_name":"Artur","last_name":"Osyczka","full_name":"Osyczka, Artur"},{"full_name":"Róg, Tomasz","last_name":"Róg","first_name":"Tomasz"}]},{"status":"public","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047203/"}],"date_created":"2018-12-11T11:51:06Z","type":"journal_article","_id":"1277","author":[{"full_name":"Ortiz Morea, Fausto","last_name":"Ortiz Morea","first_name":"Fausto"},{"last_name":"Savatin","first_name":"Daniel","full_name":"Savatin, Daniel"},{"full_name":"Dejonghe, Wim","last_name":"Dejonghe","first_name":"Wim"},{"full_name":"Kumar, Rahul","first_name":"Rahul","last_name":"Kumar"},{"first_name":"Yu","last_name":"Luo","full_name":"Luo, Yu"},{"last_name":"Adamowski","first_name":"Maciek","orcid":"0000-0001-6463-5257","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek"},{"last_name":"Van Begin","first_name":"Jos","full_name":"Van Begin, Jos"},{"full_name":"Dressano, Keini","first_name":"Keini","last_name":"Dressano"},{"first_name":"Guilherme","last_name":"De Oliveira","full_name":"De Oliveira, Guilherme"},{"full_name":"Zhao, Xiuyang","first_name":"Xiuyang","last_name":"Zhao"},{"full_name":"Lu, Qing","first_name":"Qing","last_name":"Lu"},{"first_name":"Annemieke","last_name":"Madder","full_name":"Madder, Annemieke"},{"first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"},{"last_name":"De Moura","first_name":"Daniel","full_name":"De Moura, Daniel"},{"last_name":"Russinova","first_name":"Eugenia","full_name":"Russinova, Eugenia"}],"quality_controlled":"1","oa_version":"Preprint","language":[{"iso":"eng"}],"volume":113,"month":"09","issue":"39","acknowledgement":"F.A.O.-M. was supported by special\r\nresearch funding from the Flemish Government for a joint doctorate fellowship\r\nat Ghent University, and funding from the Student Program\r\n–\r\nGraduate Studies\r\nPlan Program from the Coordination for the Improvement of Higher Educa-\r\ntion Personnel, Brazil, for a doctorate fellowship at the University of São Paulo.\r\nX.Z. and Q.L. are indebted to the China Science Council and G.P.d.O. to the\r\n“\r\nCiência sem Fronteiras\r\n”\r\nfor predoctoral fellowships. R.K. and Y.L. have re-\r\nceived postdoctoral fellowships from the Belgian Science Policy Office. This\r\nresearch was supported by Flanders Research Foundation Grant G008416N\r\n(to E.R.) and by the São Paulo Research Foundation and the National Council\r\nfor Scientific and Technological Development (CNPq) (D.S.d.M.). D.S.d.M. is a\r\nresearch fellow of CNPq.\r\nWe thank D. Van Damme, E. Mylle, M. Castro Silva-Filho,\r\nand J. Goeman for providing usefu\r\nl advice and technical assistance;\r\nI. Hara-Nishimura, J. Lin, G. Jürgens, M. A. Johnson, and P. Bozhkov for sharing\r\npublished materials; and M. Nowack and M. Fendrych for kindly donating the\r\npUBQ10::ATG8-YFP\r\n-expressing marker line.","day":"27","page":"11028 - 11033","year":"2016","doi":"10.1073/pnas.1605588113","oa":1,"publist_id":"6039","intvolume":"       113","publication":"PNAS","scopus_import":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"JiFr"}],"date_updated":"2021-01-12T06:49:34Z","citation":{"mla":"Ortiz Morea, Fausto, et al. “Danger-Associated Peptide Signaling in Arabidopsis Requires Clathrin.” <i>PNAS</i>, vol. 113, no. 39, National Academy of Sciences, 2016, pp. 11028–33, doi:<a href=\"https://doi.org/10.1073/pnas.1605588113\">10.1073/pnas.1605588113</a>.","short":"F. Ortiz Morea, D. Savatin, W. Dejonghe, R. Kumar, Y. Luo, M. Adamowski, J. Van Begin, K. Dressano, G. De Oliveira, X. Zhao, Q. Lu, A. Madder, J. Friml, D. De Moura, E. Russinova, PNAS 113 (2016) 11028–11033.","ama":"Ortiz Morea F, Savatin D, Dejonghe W, et al. Danger-associated peptide signaling in Arabidopsis requires clathrin. <i>PNAS</i>. 2016;113(39):11028-11033. doi:<a href=\"https://doi.org/10.1073/pnas.1605588113\">10.1073/pnas.1605588113</a>","ista":"Ortiz Morea F, Savatin D, Dejonghe W, Kumar R, Luo Y, Adamowski M, Van Begin J, Dressano K, De Oliveira G, Zhao X, Lu Q, Madder A, Friml J, De Moura D, Russinova E. 2016. Danger-associated peptide signaling in Arabidopsis requires clathrin. PNAS. 113(39), 11028–11033.","chicago":"Ortiz Morea, Fausto, Daniel Savatin, Wim Dejonghe, Rahul Kumar, Yu Luo, Maciek Adamowski, Jos Van Begin, et al. “Danger-Associated Peptide Signaling in Arabidopsis Requires Clathrin.” <i>PNAS</i>. National Academy of Sciences, 2016. <a href=\"https://doi.org/10.1073/pnas.1605588113\">https://doi.org/10.1073/pnas.1605588113</a>.","apa":"Ortiz Morea, F., Savatin, D., Dejonghe, W., Kumar, R., Luo, Y., Adamowski, M., … Russinova, E. (2016). Danger-associated peptide signaling in Arabidopsis requires clathrin. <i>PNAS</i>. National Academy of Sciences. <a href=\"https://doi.org/10.1073/pnas.1605588113\">https://doi.org/10.1073/pnas.1605588113</a>","ieee":"F. Ortiz Morea <i>et al.</i>, “Danger-associated peptide signaling in Arabidopsis requires clathrin,” <i>PNAS</i>, vol. 113, no. 39. National Academy of Sciences, pp. 11028–11033, 2016."},"date_published":"2016-09-27T00:00:00Z","publisher":"National Academy of Sciences","title":"Danger-associated peptide signaling in Arabidopsis requires clathrin","publication_status":"published","abstract":[{"text":"The Arabidopsis thaliana endogenous elicitor peptides (AtPeps) are released into the apoplast after cellular damage caused by pathogens or wounding to induce innate immunity by direct binding to the membrane-localized leucine-rich repeat receptor kinases, PEP RECEPTOR1 (PEPR1) and PEPR2. Although the PEPR-mediated signaling components and responses have been studied extensively, the contributions of the subcellular localization and dynamics of the active PEPRs remain largely unknown. We used live-cell imaging of the fluorescently labeled and bioactive pep1 to visualize the intracellular behavior of the PEPRs in the Arabidopsis root meristem. We found that AtPep1 decorated the plasma membrane (PM) in a receptor-dependent manner and cointernalized with PEPRs. Trafficking of the AtPep1-PEPR1 complexes to the vacuole required neither the trans-Golgi network/early endosome (TGN/EE)-localized vacuolar H+ -ATPase activity nor the function of the brefeldin A-sensitive ADP-ribosylation factor-guanine exchange factors (ARF-GEFs). In addition, AtPep1 and different TGN/EE markers colocalized only rarely, implying that the intracellular route of this receptor-ligand pair is largely independent of the TGN/EE. Inducible overexpression of the Arabidopsis clathrin coat disassembly factor, Auxilin2, which inhibits clathrin-mediated endocytosis (CME), impaired the AtPep1-PEPR1 internalization and compromised AtPep1-mediated responses. Our results show that clathrin function at the PM is required to induce plant defense responses, likely through CME of cell surface-located signaling components.\r\n","lang":"eng"}]},{"title":"Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies","publisher":"Public Library of Science","date_published":"2016-10-06T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","ddc":["570","571"],"publication_status":"published","abstract":[{"lang":"eng","text":"Adaptations of vestibulo-ocular and optokinetic response eye movements have been studied as an experimental model of cerebellum-dependent motor learning. Several previous physiological and pharmacological studies have consistently suggested that the cerebellar flocculus (FL) Purkinje cells (P-cells) and the medial vestibular nucleus (MVN) neurons targeted by FL (FL-targeted MVN neurons) may respectively maintain the memory traces of short- and long-term adaptation. To study the basic structures of the FL-MVN synapses by light microscopy (LM) and electron microscopy (EM), we injected green florescence protein (GFP)-expressing lentivirus into FL to anterogradely label the FL P-cell axons in C57BL/6J mice. The FL P-cell axonal boutons were distributed in the magnocellular MVN and in the border region of parvocellular MVN and prepositus hypoglossi (PrH). In the magnocellular MVN, the FL-P cell axons mainly terminated on somata and proximal dendrites. On the other hand, in the parvocellular MVN/PrH, the FL P-cell axonal synaptic boutons mainly terminated on the relatively small-diameter (&lt; 1 μm) distal dendrites of MVN neurons, forming symmetrical synapses. The majority of such parvocellular MVN/PrH neurons were determined to be glutamatergic by immunocytochemistry and in-situ hybridization of GFP expressing transgenic mice. To further examine the spatial relationship between the synapses of FL P-cells and those of vestibular nerve on the neurons of the parvocellular MVN/ PrH, we added injections of biotinylated dextran amine into the semicircular canal and anterogradely labeled vestibular nerve axons in some mice. The MVN dendrites receiving the FL P-cell axonal synaptic boutons often closely apposed vestibular nerve synaptic boutons in both LM and EM studies. Such a partial overlap of synaptic boutons of FL P-cell axons with those of vestibular nerve axons in the distal dendrites of MVN neurons suggests that inhibitory synapses of FL P-cells may influence the function of neighboring excitatory synapses of vestibular nerve in the parvocellular MVN/PrH neurons."}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"citation":{"mla":"Matsuno, Hitomi, et al. “Distribution and Structure of Synapses on Medial Vestibular Nuclear Neurons Targeted by Cerebellar Flocculus Purkinje Cells and Vestibular Nerve in Mice: Light and Electron Microscopy Studies.” <i>PLoS One</i>, vol. 11, no. 10, e0164037, Public Library of Science, 2016, doi:<a href=\"https://doi.org/10.1371/journal.pone.0164037\">10.1371/journal.pone.0164037</a>.","short":"H. Matsuno, M. Kudoh, A. Watakabe, T. Yamamori, R. Shigemoto, S. Nagao, PLoS One 11 (2016).","apa":"Matsuno, H., Kudoh, M., Watakabe, A., Yamamori, T., Shigemoto, R., &#38; Nagao, S. (2016). Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0164037\">https://doi.org/10.1371/journal.pone.0164037</a>","ama":"Matsuno H, Kudoh M, Watakabe A, Yamamori T, Shigemoto R, Nagao S. Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies. <i>PLoS One</i>. 2016;11(10). doi:<a href=\"https://doi.org/10.1371/journal.pone.0164037\">10.1371/journal.pone.0164037</a>","ista":"Matsuno H, Kudoh M, Watakabe A, Yamamori T, Shigemoto R, Nagao S. 2016. Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies. PLoS One. 11(10), e0164037.","chicago":"Matsuno, Hitomi, Moeko Kudoh, Akiya Watakabe, Tetsuo Yamamori, Ryuichi Shigemoto, and Soichi Nagao. “Distribution and Structure of Synapses on Medial Vestibular Nuclear Neurons Targeted by Cerebellar Flocculus Purkinje Cells and Vestibular Nerve in Mice: Light and Electron Microscopy Studies.” <i>PLoS One</i>. Public Library of Science, 2016. <a href=\"https://doi.org/10.1371/journal.pone.0164037\">https://doi.org/10.1371/journal.pone.0164037</a>.","ieee":"H. Matsuno, M. Kudoh, A. Watakabe, T. Yamamori, R. Shigemoto, and S. Nagao, “Distribution and structure of synapses on medial vestibular nuclear neurons targeted by cerebellar flocculus purkinje cells and vestibular nerve in mice: Light and electron microscopy studies,” <i>PLoS One</i>, vol. 11, no. 10. Public Library of Science, 2016."},"department":[{"_id":"RySh"}],"date_updated":"2021-01-12T06:49:34Z","publication":"PLoS One","publist_id":"6038","intvolume":"        11","scopus_import":1,"doi":"10.1371/journal.pone.0164037","day":"06","year":"2016","acknowledgement":"This work was supported by RIKEN [to SN]; Grant-in-Aid from the Japan Society for the Promotion of Science, https://www.jsps.go.jp/english/e-grants/ [22300112 to SN].","oa":1,"volume":11,"article_processing_charge":"No","language":[{"iso":"eng"}],"file":[{"file_size":3657084,"access_level":"open_access","creator":"system","checksum":"7c0ba0ca6d79844059158059d2a38d25","file_id":"5269","relation":"main_file","file_name":"IST-2016-689-v1+1_journal.pone.0164037.PDF","date_updated":"2020-07-14T12:44:42Z","date_created":"2018-12-12T10:17:16Z","content_type":"application/pdf"}],"article_number":"e0164037","issue":"10","has_accepted_license":"1","month":"10","author":[{"full_name":"Matsuno, Hitomi","last_name":"Matsuno","first_name":"Hitomi"},{"last_name":"Kudoh","first_name":"Moeko","full_name":"Kudoh, Moeko"},{"first_name":"Akiya","last_name":"Watakabe","full_name":"Watakabe, Akiya"},{"full_name":"Yamamori, Tetsuo","last_name":"Yamamori","first_name":"Tetsuo"},{"full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","last_name":"Shigemoto"},{"first_name":"Soichi","last_name":"Nagao","full_name":"Nagao, Soichi"}],"oa_version":"Published Version","quality_controlled":"1","pubrep_id":"689","status":"public","article_type":"original","type":"journal_article","_id":"1278","date_created":"2018-12-11T11:51:06Z"},{"pubrep_id":"690","ec_funded":1,"type":"journal_article","_id":"1279","date_created":"2018-12-11T11:51:06Z","status":"public","issue":"10","file":[{"date_created":"2018-12-12T10:13:26Z","date_updated":"2020-07-14T12:44:42Z","content_type":"application/pdf","file_name":"IST-2016-690-v1+1_journal.pone.0164675.PDF","relation":"main_file","access_level":"open_access","file_size":4353592,"checksum":"395895ecb2216e9c39135abaa56b28b3","file_id":"5009","creator":"system"}],"article_number":"e0164675","month":"10","has_accepted_license":"1","volume":11,"language":[{"iso":"eng"}],"oa_version":"Published Version","quality_controlled":"1","project":[{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"call_identifier":"FP7","grant_number":"281511","_id":"257A4776-B435-11E9-9278-68D0E5697425","name":"Memory-related information processing in neuronal circuits of the hippocampus and entorhinal cortex"}],"author":[{"first_name":"Krisztián","last_name":"Kovács","id":"2AB5821E-F248-11E8-B48F-1D18A9856A87","full_name":"Kovács, Krisztián"},{"id":"426376DC-F248-11E8-B48F-1D18A9856A87","full_name":"O'Neill, Joseph","first_name":"Joseph","last_name":"O'Neill"},{"full_name":"Schönenberger, Philipp","id":"3B9D816C-F248-11E8-B48F-1D18A9856A87","first_name":"Philipp","last_name":"Schönenberger"},{"full_name":"Penttonen, Markku","first_name":"Markku","last_name":"Penttonen"},{"full_name":"Rangel Guerrero, Dámaris K","id":"4871BCE6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8602-4374","first_name":"Dámaris K","last_name":"Rangel Guerrero"},{"orcid":"0000-0002-5193-4036","first_name":"Jozsef L","last_name":"Csicsvari","full_name":"Csicsvari, Jozsef L","id":"3FA14672-F248-11E8-B48F-1D18A9856A87"}],"scopus_import":1,"publication":"PLoS One","intvolume":"        11","publist_id":"6037","oa":1,"year":"2016","doi":"10.1371/journal.pone.0164675","day":"19","acknowledgement":"The research leading to these results has received funding from the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement n° [291734] via the IST FELLOWSHIP awarded to Dr. Krisztián A. Kovács and the European Research Council starting grant (acronym: HIPECMEM Project reference: 281511) awarded to Dr. Jozsef Csicsvari. We thank Lauri Viljanto for technical help in building the ripple detector.","ddc":["570","571"],"abstract":[{"lang":"eng","text":"During hippocampal sharp wave/ripple (SWR) events, previously occurring, sensory inputdriven neuronal firing patterns are replayed. Such replay is thought to be important for plasticity- related processes and consolidation of memory traces. It has previously been shown that the electrical stimulation-induced disruption of SWR events interferes with learning in rodents in different experimental paradigms. On the other hand, the cognitive map theory posits that the plastic changes of the firing of hippocampal place cells constitute the electrophysiological counterpart of the spatial learning, observable at the behavioral level. Therefore, we tested whether intact SWR events occurring during the sleep/rest session after the first exploration of a novel environment are needed for the stabilization of the CA1 code, which process requires plasticity. We found that the newly-formed representation in the CA1 has the same level of stability with optogenetic SWR blockade as with a control manipulation that delivered the same amount of light into the brain. Therefore our results suggest that at least in the case of passive exploratory behavior, SWR-related plasticity is dispensable for the stability of CA1 ensembles."}],"publication_status":"published","title":"Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus","publisher":"Public Library of Science","date_published":"2016-10-19T00:00:00Z","file_date_updated":"2020-07-14T12:44:42Z","citation":{"ieee":"K. Kovács, J. O’Neill, P. Schönenberger, M. Penttonen, D. K. Rangel Guerrero, and J. L. Csicsvari, “Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus,” <i>PLoS One</i>, vol. 11, no. 10. Public Library of Science, 2016.","apa":"Kovács, K., O’Neill, J., Schönenberger, P., Penttonen, M., Rangel Guerrero, D. K., &#38; Csicsvari, J. L. (2016). Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0164675\">https://doi.org/10.1371/journal.pone.0164675</a>","ama":"Kovács K, O’Neill J, Schönenberger P, Penttonen M, Rangel Guerrero DK, Csicsvari JL. Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus. <i>PLoS One</i>. 2016;11(10). doi:<a href=\"https://doi.org/10.1371/journal.pone.0164675\">10.1371/journal.pone.0164675</a>","ista":"Kovács K, O’Neill J, Schönenberger P, Penttonen M, Rangel Guerrero DK, Csicsvari JL. 2016. Optogenetically blocking sharp wave ripple events in sleep does not interfere with the formation of stable spatial representation in the CA1 area of the hippocampus. PLoS One. 11(10), e0164675.","chicago":"Kovács, Krisztián, Joseph O’Neill, Philipp Schönenberger, Markku Penttonen, Dámaris K Rangel Guerrero, and Jozsef L Csicsvari. “Optogenetically Blocking Sharp Wave Ripple Events in Sleep Does Not Interfere with the Formation of Stable Spatial Representation in the CA1 Area of the Hippocampus.” <i>PLoS One</i>. Public Library of Science, 2016. <a href=\"https://doi.org/10.1371/journal.pone.0164675\">https://doi.org/10.1371/journal.pone.0164675</a>.","mla":"Kovács, Krisztián, et al. “Optogenetically Blocking Sharp Wave Ripple Events in Sleep Does Not Interfere with the Formation of Stable Spatial Representation in the CA1 Area of the Hippocampus.” <i>PLoS One</i>, vol. 11, no. 10, e0164675, Public Library of Science, 2016, doi:<a href=\"https://doi.org/10.1371/journal.pone.0164675\">10.1371/journal.pone.0164675</a>.","short":"K. Kovács, J. O’Neill, P. Schönenberger, M. Penttonen, D.K. Rangel Guerrero, J.L. Csicsvari, PLoS One 11 (2016)."},"date_updated":"2021-01-12T06:49:35Z","department":[{"_id":"JoCs"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"citation":{"short":"P. Bourgade, L. Erdös, H. Yau, J. Yin, Communications on Pure and Applied Mathematics 69 (2016) 1815–1881.","mla":"Bourgade, Paul, et al. “Fixed Energy Universality for Generalized Wigner Matrices.” <i>Communications on Pure and Applied Mathematics</i>, vol. 69, no. 10, Wiley-Blackwell, 2016, pp. 1815–81, doi:<a href=\"https://doi.org/10.1002/cpa.21624\">10.1002/cpa.21624</a>.","chicago":"Bourgade, Paul, László Erdös, Horngtzer Yau, and Jun Yin. “Fixed Energy Universality for Generalized Wigner Matrices.” <i>Communications on Pure and Applied Mathematics</i>. Wiley-Blackwell, 2016. <a href=\"https://doi.org/10.1002/cpa.21624\">https://doi.org/10.1002/cpa.21624</a>.","ista":"Bourgade P, Erdös L, Yau H, Yin J. 2016. Fixed energy universality for generalized wigner matrices. Communications on Pure and Applied Mathematics. 69(10), 1815–1881.","ama":"Bourgade P, Erdös L, Yau H, Yin J. Fixed energy universality for generalized wigner matrices. <i>Communications on Pure and Applied Mathematics</i>. 2016;69(10):1815-1881. doi:<a href=\"https://doi.org/10.1002/cpa.21624\">10.1002/cpa.21624</a>","apa":"Bourgade, P., Erdös, L., Yau, H., &#38; Yin, J. (2016). Fixed energy universality for generalized wigner matrices. <i>Communications on Pure and Applied Mathematics</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/cpa.21624\">https://doi.org/10.1002/cpa.21624</a>","ieee":"P. Bourgade, L. Erdös, H. Yau, and J. Yin, “Fixed energy universality for generalized wigner matrices,” <i>Communications on Pure and Applied Mathematics</i>, vol. 69, no. 10. Wiley-Blackwell, pp. 1815–1881, 2016."},"department":[{"_id":"LaEr"}],"date_updated":"2021-01-12T06:49:35Z","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","publication_status":"published","abstract":[{"text":"We prove the Wigner-Dyson-Mehta conjecture at fixed energy in the bulk of the spectrum for generalized symmetric and Hermitian Wigner matrices. Previous results concerning the universality of random matrices either require an averaging in the energy parameter or they hold only for Hermitian matrices if the energy parameter is fixed. We develop a homogenization theory of the Dyson Brownian motion and show that microscopic universality follows from mesoscopic statistics.","lang":"eng"}],"title":"Fixed energy universality for generalized wigner matrices","date_published":"2016-10-01T00:00:00Z","publisher":"Wiley-Blackwell","oa":1,"page":"1815 - 1881","year":"2016","doi":"10.1002/cpa.21624","day":"01","acknowledgement":"The work of P.B. was partially supported by National Sci-\r\nence Foundation Grant DMS-1208859.  The work of L.E. was partially supported\r\nby ERC Advanced Grant RANMAT 338804.  The work of H.-T. Y. was partially\r\nsupported by National Science Foundation Grant DMS-1307444 and a Simons In-\r\nvestigator award.  The work of J.Y. was partially supported by National Science\r\nFoundation Grant DMS-1207961.  The major part of this research was conducted\r\nwhen all authors were visiting IAS and were also supported by National Science\r\nFoundation Grant DMS-1128255.","scopus_import":1,"publication":"Communications on Pure and Applied Mathematics","publist_id":"6036","intvolume":"        69","oa_version":"Preprint","project":[{"call_identifier":"FP7","grant_number":"338804","_id":"258DCDE6-B435-11E9-9278-68D0E5697425","name":"Random matrices, universality and disordered quantum systems"}],"author":[{"first_name":"Paul","last_name":"Bourgade","full_name":"Bourgade, Paul"},{"orcid":"0000-0001-5366-9603","first_name":"László","last_name":"Erdös","full_name":"Erdös, László","id":"4DBD5372-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Yau","first_name":"Horngtzer","full_name":"Yau, Horngtzer"},{"full_name":"Yin, Jun","last_name":"Yin","first_name":"Jun"}],"issue":"10","month":"10","volume":69,"language":[{"iso":"eng"}],"type":"journal_article","_id":"1280","date_created":"2018-12-11T11:51:07Z","main_file_link":[{"open_access":"1","url":"https://arxiv.org/abs/1407.5606"}],"status":"public","ec_funded":1},{"oa_version":"Preprint","quality_controlled":"1","author":[{"first_name":"Eléonore","last_name":"Bouguyon","full_name":"Bouguyon, Eléonore"},{"first_name":"Francine","last_name":"Perrine Walker","full_name":"Perrine Walker, Francine"},{"last_name":"Pervent","first_name":"Marjorie","full_name":"Pervent, Marjorie"},{"first_name":"Juliette","last_name":"Rochette","full_name":"Rochette, Juliette"},{"id":"33A3C818-F248-11E8-B48F-1D18A9856A87","full_name":"Cuesta, Candela","last_name":"Cuesta","first_name":"Candela","orcid":"0000-0003-1923-2410"},{"full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","last_name":"Benková"},{"full_name":"Martinière, Alexandre","last_name":"Martinière","first_name":"Alexandre"},{"full_name":"Bach, Lien","last_name":"Bach","first_name":"Lien"},{"first_name":"Gabriel","last_name":"Krouk","full_name":"Krouk, Gabriel"},{"full_name":"Gojon, Alain","first_name":"Alain","last_name":"Gojon"},{"first_name":"Philippe","last_name":"Nacry","full_name":"Nacry, Philippe"}],"issue":"2","month":"10","volume":172,"language":[{"iso":"eng"}],"type":"journal_article","_id":"1281","date_created":"2018-12-11T11:51:07Z","status":"public","main_file_link":[{"open_access":"1","url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5047109/"}],"citation":{"mla":"Bouguyon, Eléonore, et al. “Nitrate Controls Root Development through Posttranscriptional Regulation of the NRT1.1/NPF6.3 Transporter Sensor.” <i>Plant Physiology</i>, vol. 172, no. 2, American Society of Plant Biologists, 2016, pp. 1237–48, doi:<a href=\"https://doi.org/10.1104/pp.16.01047\">10.1104/pp.16.01047</a>.","short":"E. Bouguyon, F. Perrine Walker, M. Pervent, J. Rochette, C. Cuesta, E. Benková, A. Martinière, L. Bach, G. Krouk, A. Gojon, P. Nacry, Plant Physiology 172 (2016) 1237–1248.","ieee":"E. Bouguyon <i>et al.</i>, “Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor,” <i>Plant Physiology</i>, vol. 172, no. 2. American Society of Plant Biologists, pp. 1237–1248, 2016.","chicago":"Bouguyon, Eléonore, Francine Perrine Walker, Marjorie Pervent, Juliette Rochette, Candela Cuesta, Eva Benková, Alexandre Martinière, et al. “Nitrate Controls Root Development through Posttranscriptional Regulation of the NRT1.1/NPF6.3 Transporter Sensor.” <i>Plant Physiology</i>. American Society of Plant Biologists, 2016. <a href=\"https://doi.org/10.1104/pp.16.01047\">https://doi.org/10.1104/pp.16.01047</a>.","ama":"Bouguyon E, Perrine Walker F, Pervent M, et al. Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor. <i>Plant Physiology</i>. 2016;172(2):1237-1248. doi:<a href=\"https://doi.org/10.1104/pp.16.01047\">10.1104/pp.16.01047</a>","ista":"Bouguyon E, Perrine Walker F, Pervent M, Rochette J, Cuesta C, Benková E, Martinière A, Bach L, Krouk G, Gojon A, Nacry P. 2016. Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor. Plant Physiology. 172(2), 1237–1248.","apa":"Bouguyon, E., Perrine Walker, F., Pervent, M., Rochette, J., Cuesta, C., Benková, E., … Nacry, P. (2016). Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor. <i>Plant Physiology</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1104/pp.16.01047\">https://doi.org/10.1104/pp.16.01047</a>"},"date_updated":"2021-01-12T06:49:36Z","department":[{"_id":"EvBe"}],"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"Plants are able to modulate root growth and development to optimize their nitrogen nutrition. In Arabidopsis (Arabidopsis thaliana), the adaptive root response to nitrate (NO3 -) depends on the NRT1.1/NPF6.3 transporter/sensor. NRT1.1 represses emergence of lateral root primordia (LRPs) at low concentration or absence of NO3 - through its auxin transport activity that lowers auxin accumulation in LR. However, these functional data strongly contrast with the known transcriptional regulation of NRT1.1, which is markedly repressed in LRPs in the absence of NO3 -. To explain this discrepancy, we investigated in detail the spatiotemporal expression pattern of the NRT1.1 protein during LRP development and combined local transcript analysis with the use of transgenic lines expressing tagged NRT1.1 proteins. Our results show that although NO3 - stimulates NRT1.1 transcription and probably mRNA stability both in primary root tissues and in LRPs, it acts differentially on protein accumulation, depending on the tissues considered with stimulation in cortex and epidermis of the primary root and a strong repression in LRPs and to a lower extent at the primary root tip. This demonstrates that NRT1.1 is strongly regulated at the posttranscriptional level by tissue-specific mechanisms. These mechanisms are crucial for controlling the large palette of adaptive responses to NO3 - mediated by NRT1.1 as they ensure that the protein is present in the proper tissue under the specific conditions where it plays a signaling role in this particular tissue.","lang":"eng"}],"publication_status":"published","title":"Nitrate controls root development through posttranscriptional regulation of the NRT1.1/NPF6.3 transporter sensor","date_published":"2016-10-01T00:00:00Z","publisher":"American Society of Plant Biologists","oa":1,"day":"01","doi":"10.1104/pp.16.01047","page":"1237 - 1248","year":"2016","acknowledgement":"This work was supported by the Agropolis Foundation (RHIZOPOLIS project to A.G. and P.N., and RTRA 2009-2011 project to F.P.-W.), the Knowledge Biobase Economy European project (KBBE-005-002 Root enhancement for crop improvement to M.P. and P.N.), and the European EURoot project (FP7-KBBE-2011-5 to J.R., A.G., and P.N.). We thank Carine Alcon for the help with analysis of confocal images, Xavier\r\nDumont for assistance with Arabidopsis transformations, staff members of the\r\nInstitut de Biologie Intégrative des Plantes for technical assistance with biological\r\nmaterial culture, and students and trainees for assistance with laboratory work.\r\nConfocal observations were made at the Montpellier RIO Imaging facility.","scopus_import":1,"publication":"Plant Physiology","intvolume":"       172","publist_id":"6035"}]