[{"citation":{"short":"A.F. Leithner, L. Altenburger, R. Hauschild, F.P. Assen, K. Rottner, S. TEB, A. Diz-Muñoz, J. Stein, M.K. Sixt, Journal of Cell Biology 220 (2021).","ama":"Leithner AF, Altenburger L, Hauschild R, et al. Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse. <i>Journal of Cell Biology</i>. 2021;220(4). doi:<a href=\"https://doi.org/10.1083/jcb.202006081\">10.1083/jcb.202006081</a>","ista":"Leithner AF, Altenburger L, Hauschild R, Assen FP, Rottner K, TEB S, Diz-Muñoz A, Stein J, Sixt MK. 2021. Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse. Journal of Cell Biology. 220(4), e202006081.","apa":"Leithner, A. F., Altenburger, L., Hauschild, R., Assen, F. P., Rottner, K., TEB, S., … Sixt, M. K. (2021). Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202006081\">https://doi.org/10.1083/jcb.202006081</a>","chicago":"Leithner, Alexander F, LM Altenburger, R Hauschild, Frank P Assen, K Rottner, Stradal TEB, A Diz-Muñoz, JV Stein, and Michael K Sixt. “Dendritic Cell Actin Dynamics Control Contact Duration and Priming Efficiency at the Immunological Synapse.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2021. <a href=\"https://doi.org/10.1083/jcb.202006081\">https://doi.org/10.1083/jcb.202006081</a>.","ieee":"A. F. Leithner <i>et al.</i>, “Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse,” <i>Journal of Cell Biology</i>, vol. 220, no. 4. Rockefeller University Press, 2021.","mla":"Leithner, Alexander F., et al. “Dendritic Cell Actin Dynamics Control Contact Duration and Priming Efficiency at the Immunological Synapse.” <i>Journal of Cell Biology</i>, vol. 220, no. 4, e202006081, Rockefeller University Press, 2021, doi:<a href=\"https://doi.org/10.1083/jcb.202006081\">10.1083/jcb.202006081</a>."},"intvolume":"       220","day":"05","has_accepted_license":"1","article_number":"e202006081","status":"public","abstract":[{"text":"Dendritic cells (DCs) are crucial for the priming of naive T cells and the initiation of adaptive immunity. Priming is initiated at a heterologous cell–cell contact, the immunological synapse (IS). While it is established that F-actin dynamics regulates signaling at the T cell side of the contact, little is known about the cytoskeletal contribution on the DC side. Here, we show that the DC actin cytoskeleton is decisive for the formation of a multifocal synaptic structure, which correlates with T cell priming efficiency. DC actin at the IS appears in transient foci that are dynamized by the WAVE regulatory complex (WRC). The absence of the WRC in DCs leads to stabilized contacts with T cells, caused by an increase in ICAM1-integrin–mediated cell–cell adhesion. This results in lower numbers of activated and proliferating T cells, demonstrating an important role for DC actin in the regulation of immune synapse functionality.","lang":"eng"}],"department":[{"_id":"MiSi"}],"title":"Dendritic cell actin dynamics control contact duration and priming efficiency at the immunological synapse","oa":1,"publication_status":"published","article_type":"original","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","_id":"9094","month":"04","publication_identifier":{"eissn":["1540-8140"],"issn":["0021-9525"]},"scopus_import":"1","pmid":1,"article_processing_charge":"No","tmp":{"short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)"},"volume":220,"file":[{"file_size":5102328,"creator":"dernst","relation":"main_file","checksum":"843ebc153847c8626e13c9c5ce71d533","date_created":"2022-05-12T14:16:21Z","content_type":"application/pdf","file_id":"11367","success":1,"date_updated":"2022-05-12T14:16:21Z","access_level":"open_access","file_name":"2021_JournCellBiology_Leithner.pdf"}],"isi":1,"quality_controlled":"1","year":"2021","date_created":"2021-02-05T10:08:04Z","date_updated":"2023-09-05T13:57:53Z","publication":"Journal of Cell Biology","issue":"4","ddc":["570"],"author":[{"id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-1073-744X","last_name":"Leithner","first_name":"Alexander F","full_name":"Leithner, Alexander F"},{"last_name":"Altenburger","full_name":"Altenburger, LM","first_name":"LM"},{"last_name":"Hauschild","first_name":"R","full_name":"Hauschild, R"},{"orcid":"0000-0003-3470-6119","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","full_name":"Assen, Frank P","first_name":"Frank P","last_name":"Assen"},{"full_name":"Rottner, K","first_name":"K","last_name":"Rottner"},{"full_name":"TEB, Stradal","first_name":"Stradal","last_name":"TEB"},{"last_name":"Diz-Muñoz","first_name":"A","full_name":"Diz-Muñoz, A"},{"first_name":"JV","full_name":"Stein, JV","last_name":"Stein"},{"last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"external_id":{"pmid":["33533935"],"isi":["000626365700001"]},"license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","oa_version":"Published Version","date_published":"2021-04-05T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2022-05-12T14:16:21Z","type":"journal_article","doi":"10.1083/jcb.202006081","publisher":"Rockefeller University Press"},{"year":"2021","date_created":"2021-03-21T23:01:20Z","quality_controlled":"1","project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373","name":"Cellular navigation along spatial gradients","call_identifier":"H2020"},{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"Y 564-B12","_id":"25A8E5EA-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"}],"date_updated":"2023-08-07T14:18:26Z","file":[{"date_updated":"2021-03-22T12:08:26Z","access_level":"open_access","success":1,"date_created":"2021-03-22T12:08:26Z","file_id":"9277","content_type":"application/pdf","file_name":"2021_FrontiersImmumo_Vaahtomeri.pdf","creator":"dernst","file_size":3740146,"checksum":"663f5a48375e42afa4bfef58d42ec186","relation":"main_file"}],"isi":1,"volume":12,"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"publication_identifier":{"eissn":["1664-3224"]},"pmid":1,"scopus_import":"1","doi":"10.3389/fimmu.2021.630002","publisher":"Frontiers","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2021-03-22T12:08:26Z","oa_version":"Published Version","license":"https://creativecommons.org/licenses/by/4.0/","external_id":{"isi":["000627134400001"],"pmid":["33717158"]},"date_published":"2021-02-25T00:00:00Z","publication":"Frontiers in Immunology","author":[{"last_name":"Vaahtomeri","first_name":"Kari","full_name":"Vaahtomeri, Kari","orcid":"0000-0001-7829-3518","id":"368EE576-F248-11E8-B48F-1D18A9856A87"},{"id":"3356F664-F248-11E8-B48F-1D18A9856A87","first_name":"Christine","full_name":"Moussion, Christine","last_name":"Moussion"},{"last_name":"Hauschild","full_name":"Hauschild, Robert","first_name":"Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt"}],"ddc":["570"],"ec_funded":1,"status":"public","article_number":"630002","abstract":[{"text":"Gradients of chemokines and growth factors guide migrating cells and morphogenetic processes. Migration of antigen-presenting dendritic cells from the interstitium into the lymphatic system is dependent on chemokine CCL21, which is secreted by endothelial cells of the lymphatic capillary, binds heparan sulfates and forms gradients decaying into the interstitium. Despite the importance of CCL21 gradients, and chemokine gradients in general, the mechanisms of gradient formation are unclear. Studies on fibroblast growth factors have shown that limited diffusion is crucial for gradient formation. Here, we used the mouse dermis as a model tissue to address the necessity of CCL21 anchoring to lymphatic capillary heparan sulfates in the formation of interstitial CCL21 gradients. Surprisingly, the absence of lymphatic endothelial heparan sulfates resulted only in a modest decrease of CCL21 levels at the lymphatic capillaries and did neither affect interstitial CCL21 gradient shape nor dendritic cell migration toward lymphatic capillaries. Thus, heparan sulfates at the level of the lymphatic endothelium are dispensable for the formation of a functional CCL21 gradient.","lang":"eng"}],"day":"25","has_accepted_license":"1","citation":{"apa":"Vaahtomeri, K., Moussion, C., Hauschild, R., &#38; Sixt, M. K. (2021). Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. <i>Frontiers in Immunology</i>. Frontiers. <a href=\"https://doi.org/10.3389/fimmu.2021.630002\">https://doi.org/10.3389/fimmu.2021.630002</a>","ista":"Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. 2021. Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. Frontiers in Immunology. 12, 630002.","chicago":"Vaahtomeri, Kari, Christine Moussion, Robert Hauschild, and Michael K Sixt. “Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.” <i>Frontiers in Immunology</i>. Frontiers, 2021. <a href=\"https://doi.org/10.3389/fimmu.2021.630002\">https://doi.org/10.3389/fimmu.2021.630002</a>.","mla":"Vaahtomeri, Kari, et al. “Shape and Function of Interstitial Chemokine CCL21 Gradients Are Independent of Heparan Sulfates Produced by Lymphatic Endothelium.” <i>Frontiers in Immunology</i>, vol. 12, 630002, Frontiers, 2021, doi:<a href=\"https://doi.org/10.3389/fimmu.2021.630002\">10.3389/fimmu.2021.630002</a>.","ieee":"K. Vaahtomeri, C. Moussion, R. Hauschild, and M. K. Sixt, “Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium,” <i>Frontiers in Immunology</i>, vol. 12. Frontiers, 2021.","short":"K. Vaahtomeri, C. Moussion, R. Hauschild, M.K. Sixt, Frontiers in Immunology 12 (2021).","ama":"Vaahtomeri K, Moussion C, Hauschild R, Sixt MK. Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium. <i>Frontiers in Immunology</i>. 2021;12. doi:<a href=\"https://doi.org/10.3389/fimmu.2021.630002\">10.3389/fimmu.2021.630002</a>"},"intvolume":"        12","_id":"9259","month":"02","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","title":"Shape and function of interstitial chemokine CCL21 gradients are independent of heparan sulfates produced by lymphatic endothelium","oa":1,"article_type":"original","department":[{"_id":"MiSi"},{"_id":"Bio"}],"acknowledgement":"This work was supported by Sigrid Juselius fellowship (KV), University of Helsinki 3-year research grant (KV), Academy of Finland Research fellow funding (315710, to KV), the European Research Council (ERC CoG 724373 to MS), and by the Austrian Science foundation (FWF) (Y564-B12 START award to MS).\r\nTaija Mäkinen is acknowledged for providing Prox1CreERT2 transgenic mice and Yu Yamaguchi for providing the conditional Ext1 mouse strain."},{"citation":{"mla":"Gärtner, Florian R., and Michael K. Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>, vol. 56, no. 6, Elsevier, 2021, pp. 723–25, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>.","ieee":"F. R. Gärtner and M. K. Sixt, “Engaging the front wheels to drive through fibrous terrain,” <i>Developmental Cell</i>, vol. 56, no. 6. Elsevier, pp. 723–725, 2021.","ista":"Gärtner FR, Sixt MK. 2021. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 56(6), 723–725.","apa":"Gärtner, F. R., &#38; Sixt, M. K. (2021). Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>","chicago":"Gärtner, Florian R, and Michael K Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>.","ama":"Gärtner FR, Sixt MK. Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. 2021;56(6):723-725. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>","short":"F.R. Gärtner, M.K. Sixt, Developmental Cell 56 (2021) 723–725."},"intvolume":"        56","day":"22","abstract":[{"text":"In this issue of Developmental Cell, Doyle and colleagues identify periodic anterior contraction as a characteristic feature of fibroblasts and mesenchymal cancer cells embedded in 3D collagen gels. This contractile mechanism generates a matrix prestrain required for crawling in fibrous 3D environments.","lang":"eng"}],"status":"public","department":[{"_id":"MiSi"}],"article_type":"original","publication_status":"published","title":"Engaging the front wheels to drive through fibrous terrain","oa":1,"month":"03","_id":"9294","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","pmid":1,"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2021.03.002","open_access":"1"}],"scopus_import":"1","publication_identifier":{"eissn":["18781551"],"issn":["15345807"]},"article_processing_charge":"No","isi":1,"volume":56,"date_updated":"2023-08-07T14:26:47Z","date_created":"2021-03-28T22:01:41Z","year":"2021","page":"723-725","quality_controlled":"1","author":[{"orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87","full_name":"Gärtner, Florian R","first_name":"Florian R","last_name":"Gärtner"},{"last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"publication":"Developmental Cell","issue":"6","date_published":"2021-03-22T00:00:00Z","oa_version":"Published Version","external_id":{"pmid":["33756118"],"isi":["000631681200004"]},"type":"journal_article","language":[{"iso":"eng"}],"publisher":"Elsevier","doi":"10.1016/j.devcel.2021.03.002"},{"author":[{"last_name":"Tomasek","full_name":"Tomasek, Kathrin","first_name":"Kathrin","id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3768-877X"}],"ddc":["570"],"oa_version":"Published Version","date_published":"2021-11-18T00:00:00Z","language":[{"iso":"eng"}],"type":"dissertation","file_date_updated":"2022-12-20T23:30:05Z","doi":"10.15479/at:ista:10307","publisher":"Institute of Science and Technology Austria","publication_identifier":{"issn":["2663-337X"]},"article_processing_charge":"No","file":[{"creator":"ktomasek","file_size":13266088,"checksum":"b39c9e0ef18d0484d537a67551effd02","relation":"main_file","access_level":"open_access","date_updated":"2022-12-20T23:30:05Z","content_type":"application/pdf","file_id":"10308","date_created":"2021-11-18T15:07:31Z","embargo":"2022-11-18","file_name":"ThesisTomasekKathrin.pdf"},{"file_name":"ThesisTomasekKathrin.docx","file_id":"10309","date_created":"2021-11-18T15:07:46Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","embargo_to":"open_access","date_updated":"2022-12-20T23:30:05Z","access_level":"closed","relation":"source_file","checksum":"c0c440ee9e5ef1102a518a4f9f023e7c","file_size":7539509,"creator":"ktomasek"}],"date_created":"2021-11-18T15:05:06Z","year":"2021","page":"73","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"date_updated":"2023-09-07T13:34:38Z","alternative_title":["ISTA Thesis"],"department":[{"_id":"MiSi"},{"_id":"CaGu"},{"_id":"GradSch"}],"publication_status":"published","title":"Pathogenic Escherichia coli hijack the host immune response","related_material":{"record":[{"id":"10316","relation":"part_of_dissertation","status":"public"}]},"oa":1,"_id":"10307","month":"11","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","supervisor":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K"},{"full_name":"Guet, Calin C","first_name":"Calin C","last_name":"Guet","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87"}],"citation":{"short":"K. Tomasek, Pathogenic Escherichia Coli Hijack the Host Immune Response, Institute of Science and Technology Austria, 2021.","ama":"Tomasek K. Pathogenic Escherichia coli hijack the host immune response. 2021. doi:<a href=\"https://doi.org/10.15479/at:ista:10307\">10.15479/at:ista:10307</a>","ista":"Tomasek K. 2021. Pathogenic Escherichia coli hijack the host immune response. Institute of Science and Technology Austria.","chicago":"Tomasek, Kathrin. “Pathogenic Escherichia Coli Hijack the Host Immune Response.” Institute of Science and Technology Austria, 2021. <a href=\"https://doi.org/10.15479/at:ista:10307\">https://doi.org/10.15479/at:ista:10307</a>.","apa":"Tomasek, K. (2021). <i>Pathogenic Escherichia coli hijack the host immune response</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/at:ista:10307\">https://doi.org/10.15479/at:ista:10307</a>","mla":"Tomasek, Kathrin. <i>Pathogenic Escherichia Coli Hijack the Host Immune Response</i>. Institute of Science and Technology Austria, 2021, doi:<a href=\"https://doi.org/10.15479/at:ista:10307\">10.15479/at:ista:10307</a>.","ieee":"K. Tomasek, “Pathogenic Escherichia coli hijack the host immune response,” Institute of Science and Technology Austria, 2021."},"day":"18","has_accepted_license":"1","status":"public","abstract":[{"lang":"eng","text":"Bacteria-host interactions represent a continuous trade-off between benefit and risk. Thus, the host immune response is faced with a non-trivial problem – accommodate beneficial commensals and remove harmful pathogens. This is especially difficult as molecular patterns, such as lipopolysaccharide or specific surface organelles such as pili, are conserved in both, commensal and pathogenic bacteria. Type 1 pili, tightly regulated by phase variation, are considered an important virulence factor of pathogenic bacteria as they facilitate invasion into host cells. While invasion represents a de facto passive mechanism for pathogens to escape the host immune response, we demonstrate a fundamental role of type 1 pili as active modulators of the innate and adaptive immune response."}],"degree_awarded":"PhD"},{"author":[{"id":"3AEC8556-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3768-877X","last_name":"Tomasek","full_name":"Tomasek, Kathrin","first_name":"Kathrin"},{"orcid":"0000-0002-1073-744X","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander F","full_name":"Leithner, Alexander F","last_name":"Leithner"},{"id":"727b3c7d-4939-11ec-89b3-b9b0750ab74d","full_name":"Glatzová, Ivana","first_name":"Ivana","last_name":"Glatzová"},{"first_name":"Michael S.","full_name":"Lukesch, Michael S.","last_name":"Lukesch"},{"id":"47F8433E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6220-2052","last_name":"Guet","full_name":"Guet, Calin C","first_name":"Calin C"},{"first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt","orcid":"0000-0002-4561-241X","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"publication":"bioRxiv","date_published":"2021-10-18T00:00:00Z","department":[{"_id":"CaGu"},{"_id":"MiSi"}],"acknowledgement":"We thank Ulrich Dobrindt for providing UPEC strain CFT073, Vlad Gavra and Maximilian Götz, Bor Kavčič, Jonna Alanko and Eva Kiermaier for help with experiments and Robert Hauschild, Julian Stopp and Saren Tasciyan for help with data analysis. We thank the IST Austria Scientific Service Units, especially the Bioimaging facility, the Preclinical facility and the Electron microscopy facility for technical support, Jakob Wallner and all members of the Guet and Sixt lab for fruitful discussions and Daria Siekhaus for critically reading the manuscript. This work was supported by grants from the Austrian Research Promotion Agency (FEMtech 868984) to I.G., the European Research Council (CoG 724373) and the Austrian Science Fund (FWF P29911) to M.S.","oa_version":"Preprint","type":"preprint","related_material":{"record":[{"relation":"later_version","status":"public","id":"11843"},{"relation":"dissertation_contains","status":"public","id":"10307"}]},"title":"Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14","oa":1,"publication_status":"submitted","language":[{"iso":"eng"}],"publisher":"Cold Spring Harbor Laboratory","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","month":"10","_id":"10316","doi":"10.1101/2021.10.18.464770","main_file_link":[{"url":"https://www.biorxiv.org/content/10.1101/2021.10.18.464770v1","open_access":"1"}],"citation":{"ista":"Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. bioRxiv, <a href=\"https://doi.org/10.1101/2021.10.18.464770\">10.1101/2021.10.18.464770</a>.","apa":"Tomasek, K., Leithner, A. F., Glatzová, I., Lukesch, M. S., Guet, C. C., &#38; Sixt, M. K. (n.d.). Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. <i>bioRxiv</i>. Cold Spring Harbor Laboratory. <a href=\"https://doi.org/10.1101/2021.10.18.464770\">https://doi.org/10.1101/2021.10.18.464770</a>","chicago":"Tomasek, Kathrin, Alexander F Leithner, Ivana Glatzová, Michael S. Lukesch, Calin C Guet, and Michael K Sixt. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack the Host Immune Response by Binding to CD14.” <i>BioRxiv</i>. Cold Spring Harbor Laboratory, n.d. <a href=\"https://doi.org/10.1101/2021.10.18.464770\">https://doi.org/10.1101/2021.10.18.464770</a>.","ieee":"K. Tomasek, A. F. Leithner, I. Glatzová, M. S. Lukesch, C. C. Guet, and M. K. Sixt, “Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14,” <i>bioRxiv</i>. Cold Spring Harbor Laboratory.","mla":"Tomasek, Kathrin, et al. “Type 1 Piliated Uropathogenic Escherichia Coli Hijack the Host Immune Response by Binding to CD14.” <i>BioRxiv</i>, Cold Spring Harbor Laboratory, doi:<a href=\"https://doi.org/10.1101/2021.10.18.464770\">10.1101/2021.10.18.464770</a>.","short":"K. Tomasek, A.F. Leithner, I. Glatzová, M.S. Lukesch, C.C. Guet, M.K. Sixt, BioRxiv (n.d.).","ama":"Tomasek K, Leithner AF, Glatzová I, Lukesch MS, Guet CC, Sixt MK. Type 1 piliated uropathogenic Escherichia coli hijack the host immune response by binding to CD14. <i>bioRxiv</i>. doi:<a href=\"https://doi.org/10.1101/2021.10.18.464770\">10.1101/2021.10.18.464770</a>"},"article_processing_charge":"No","day":"18","abstract":[{"lang":"eng","text":"A key attribute of persistent or recurring bacterial infections is the ability of the pathogen to evade the host’s immune response. Many Enterobacteriaceae express type 1 pili, a pre-adapted virulence trait, to invade host epithelial cells and establish persistent infections. However, the molecular mechanisms and strategies by which bacteria actively circumvent the immune response of the host remain poorly understood. Here, we identified CD14, the major co-receptor for lipopolysaccharide detection, on dendritic cells as a previously undescribed binding partner of FimH, the protein located at the tip of the type 1 pilus of Escherichia coli. The FimH amino acids involved in CD14 binding are highly conserved across pathogenic and non-pathogenic strains. Binding of pathogenic bacteria to CD14 lead to reduced dendritic cell migration and blunted expression of co-stimulatory molecules, both rate-limiting factors of T cell activation. While defining an active molecular mechanism of immune evasion by pathogens, the interaction between FimH and CD14 represents a potential target to interfere with persistent and recurrent infections, such as urinary tract infections or Crohn’s disease."}],"status":"public","date_updated":"2024-03-25T23:30:19Z","ec_funded":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"project":[{"call_identifier":"H2020","name":"Cellular navigation along spatial gradients","grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425"},{"call_identifier":"FWF","name":"Mechanical adaptation of lamellipodial actin","grant_number":"P29911","_id":"26018E70-B435-11E9-9278-68D0E5697425"}],"year":"2021","date_created":"2021-11-19T12:24:16Z"},{"language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2021-05-28T12:39:43Z","doi":"10.1038/s41467-021-23123-x","publisher":"Springer Nature","issue":"1","publication":"Nature Communications","author":[{"full_name":"Morandell, Jasmin","first_name":"Jasmin","last_name":"Morandell","id":"4739D480-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Lena A","full_name":"Schwarz, Lena A","last_name":"Schwarz","id":"29A8453C-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-1843-3173","id":"36035796-5ACA-11E9-A75E-7AF2E5697425","last_name":"Basilico","full_name":"Basilico, Bernadette","first_name":"Bernadette"},{"id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X","full_name":"Tasciyan, Saren","first_name":"Saren","last_name":"Tasciyan"},{"full_name":"Dimchev, Georgi A","first_name":"Georgi A","last_name":"Dimchev","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161"},{"full_name":"Nicolas, Armel","first_name":"Armel","last_name":"Nicolas","id":"2A103192-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-1216-9105","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","first_name":"Christoph M","full_name":"Sommer, Christoph M"},{"last_name":"Kreuzinger","first_name":"Caroline","full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-9033-9096","id":"4C66542E-F248-11E8-B48F-1D18A9856A87","last_name":"Dotter","first_name":"Christoph","full_name":"Dotter, Christoph"},{"last_name":"Knaus","full_name":"Knaus, Lisa","first_name":"Lisa","id":"3B2ABCF4-F248-11E8-B48F-1D18A9856A87"},{"id":"D23090A2-9057-11EA-883A-A8396FC7A38F","last_name":"Dobler","full_name":"Dobler, Zoe","first_name":"Zoe"},{"first_name":"Emanuele","full_name":"Cacci, Emanuele","last_name":"Cacci"},{"first_name":"Florian KM","full_name":"Schur, Florian KM","last_name":"Schur","id":"48AD8942-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4790-8078"},{"last_name":"Danzl","full_name":"Danzl, Johann G","first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973"},{"last_name":"Novarino","first_name":"Gaia","full_name":"Novarino, Gaia","orcid":"0000-0002-7673-7178","id":"3E57A680-F248-11E8-B48F-1D18A9856A87"}],"ddc":["572"],"external_id":{"isi":["000658769900010"]},"oa_version":"Published Version","date_published":"2021-05-24T00:00:00Z","isi":1,"file":[{"date_created":"2021-05-28T12:39:43Z","content_type":"application/pdf","file_id":"9430","success":1,"access_level":"open_access","date_updated":"2021-05-28T12:39:43Z","file_name":"2021_NatureCommunications_Morandell.pdf","file_size":9358599,"creator":"kschuh","relation":"main_file","checksum":"337e0f7959c35ec959984cacdcb472ba"}],"volume":12,"date_created":"2021-05-28T11:49:46Z","year":"2021","quality_controlled":"1","acknowledged_ssus":[{"_id":"PreCl"}],"project":[{"name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","_id":"260C2330-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"},{"_id":"25444568-B435-11E9-9278-68D0E5697425","name":"Probing the Reversibility of Autism Spectrum Disorders by Employing in vivo and in vitro Models","grant_number":"715508","call_identifier":"H2020"},{"call_identifier":"FWF","_id":"2548AE96-B435-11E9-9278-68D0E5697425","grant_number":"W1232-B24","name":"Molecular Drug Targets"},{"_id":"05A0D778-7A3F-11EA-A408-12923DDC885E","name":"Neural stem cells in autism and epilepsy","grant_number":"F07807"},{"call_identifier":"FWF","grant_number":"I03600","name":"Optical control of synaptic function via adhesion molecules","_id":"265CB4D0-B435-11E9-9278-68D0E5697425"}],"date_updated":"2024-09-10T12:04:26Z","publication_identifier":{"eissn":["2041-1723"]},"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","publication_status":"published","oa":1,"related_material":{"record":[{"relation":"earlier_version","status":"public","id":"7800"},{"status":"public","relation":"dissertation_contains","id":"12401"}],"link":[{"url":"https://ist.ac.at/en/news/defective-gene-slows-down-brain-cells/","relation":"press_release"}]},"title":"Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development","article_type":"original","month":"05","_id":"9429","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"GaNo"},{"_id":"JoDa"},{"_id":"FlSc"},{"_id":"MiSi"},{"_id":"LifeSc"},{"_id":"Bio"}],"acknowledgement":"We thank A. Coll Manzano, F. Freeman, M. Ladron de Guevara, and A. Ç. Yahya for technical assistance, S. Deixler, A. Lepold, and A. Schlerka for the management of our animal colony, as well as M. Schunn and the Preclinical Facility team for technical assistance. We thank K. Heesom and her team at the University of Bristol Proteomics Facility for the proteomics sample preparation, data generation, and analysis support. We thank Y. B. Simon for kindly providing the plasmid for lentiviral labeling. Further, we thank M. Sixt for his advice regarding cell migration and the fruitful discussions. This work was supported by the ISTPlus postdoctoral fellowship (Grant Agreement No. 754411) to B.B., by the European Union’s Horizon 2020 research and innovation program (ERC) grant 715508 (REVERSEAUTISM), and by the Austrian Science Fund (FWF) to G.N. (DK W1232-B24 and SFB F7807-B) and to J.G.D (I3600-B27).","status":"public","article_number":"3058","abstract":[{"text":"De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 lead to autism spectrum disorder (ASD). In mouse, constitutive haploinsufficiency leads to motor coordination deficits as well as ASD-relevant social and cognitive impairments. However, induction of Cul3 haploinsufficiency later in life does not lead to ASD-relevant behaviors, pointing to an important role of Cul3 during a critical developmental window. Here we show that Cul3 is essential to regulate neuronal migration and, therefore, constitutive Cul3 heterozygous mutant mice display cortical lamination abnormalities. At the molecular level, we found that Cul3 controls neuronal migration by tightly regulating the amount of Plastin3 (Pls3), a previously unrecognized player of neural migration. Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. Finally, we provide evidence that cellular phenotypes associated with autism-linked gene haploinsufficiency can be rescued by transcriptional activation of the intact allele in vitro, offering a proof of concept for a potential therapeutic approach for ASDs.","lang":"eng"}],"ec_funded":1,"keyword":["General Biochemistry","Genetics and Molecular Biology"],"intvolume":"        12","citation":{"ama":"Morandell J, Schwarz LA, Basilico B, et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. 2021;12(1). doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>","short":"J. Morandell, L.A. Schwarz, B. Basilico, S. Tasciyan, G.A. Dimchev, A. Nicolas, C.M. Sommer, C. Kreuzinger, C. Dotter, L. Knaus, Z. Dobler, E. Cacci, F.K. Schur, J.G. Danzl, G. Novarino, Nature Communications 12 (2021).","ieee":"J. Morandell <i>et al.</i>, “Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development,” <i>Nature Communications</i>, vol. 12, no. 1. Springer Nature, 2021.","mla":"Morandell, Jasmin, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>, vol. 12, no. 1, 3058, Springer Nature, 2021, doi:<a href=\"https://doi.org/10.1038/s41467-021-23123-x\">10.1038/s41467-021-23123-x</a>.","apa":"Morandell, J., Schwarz, L. A., Basilico, B., Tasciyan, S., Dimchev, G. A., Nicolas, A., … Novarino, G. (2021). Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>","ista":"Morandell J, Schwarz LA, Basilico B, Tasciyan S, Dimchev GA, Nicolas A, Sommer CM, Kreuzinger C, Dotter C, Knaus L, Dobler Z, Cacci E, Schur FK, Danzl JG, Novarino G. 2021. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nature Communications. 12(1), 3058.","chicago":"Morandell, Jasmin, Lena A Schwarz, Bernadette Basilico, Saren Tasciyan, Georgi A Dimchev, Armel Nicolas, Christoph M Sommer, et al. “Cul3 Regulates Cytoskeleton Protein Homeostasis and Cell Migration during a Critical Window of Brain Development.” <i>Nature Communications</i>. Springer Nature, 2021. <a href=\"https://doi.org/10.1038/s41467-021-23123-x\">https://doi.org/10.1038/s41467-021-23123-x</a>."},"day":"24","has_accepted_license":"1"},{"article_processing_charge":"Yes (in subscription journal)","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","image":"/images/cc_by_nc_nd.png","short":"CC BY-NC-ND (4.0)"},"pmid":1,"scopus_import":"1","publication_identifier":{"eissn":["19448252"],"issn":["19448244"]},"project":[{"call_identifier":"H2020","_id":"25FE9508-B435-11E9-9278-68D0E5697425","grant_number":"724373","name":"Cellular navigation along spatial gradients"}],"date_updated":"2023-08-10T14:22:48Z","year":"2021","date_created":"2021-08-08T22:01:28Z","quality_controlled":"1","page":"35545–35560","isi":1,"file":[{"file_id":"9833","date_created":"2021-08-09T09:44:03Z","content_type":"application/pdf","success":1,"date_updated":"2021-08-09T09:44:03Z","access_level":"open_access","file_name":"2021_ACSAppliedMaterialsAndInterfaces_Zisis.pdf","file_size":7123293,"creator":"asandaue","relation":"main_file","checksum":"b043a91d9f9200e467b970b692687ed3"}],"volume":13,"date_published":"2021-08-04T00:00:00Z","external_id":{"pmid":["34283577"],"isi":["000683741400026"]},"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/","oa_version":"Published Version","author":[{"full_name":"Zisis, Themistoklis","first_name":"Themistoklis","last_name":"Zisis"},{"first_name":"Jan","full_name":"Schwarz, Jan","last_name":"Schwarz","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Miriam","full_name":"Balles, Miriam","last_name":"Balles"},{"last_name":"Kretschmer","full_name":"Kretschmer, Maibritt","first_name":"Maibritt"},{"id":"34E27F1C-F248-11E8-B48F-1D18A9856A87","first_name":"Maria","full_name":"Nemethova, Maria","last_name":"Nemethova"},{"id":"3464AE84-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0876-3187","full_name":"Chait, Remy P","first_name":"Remy P","last_name":"Chait"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert"},{"last_name":"Lange","first_name":"Janina","full_name":"Lange, Janina"},{"first_name":"Calin C","full_name":"Guet, Calin C","last_name":"Guet","orcid":"0000-0001-6220-2052","id":"47F8433E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-4561-241X"},{"full_name":"Zahler, Stefan","first_name":"Stefan","last_name":"Zahler"}],"ddc":["620","570"],"issue":"30","publication":"ACS Applied Materials and Interfaces","publisher":"American Chemical Society","doi":"10.1021/acsami.1c09850","type":"journal_article","file_date_updated":"2021-08-09T09:44:03Z","language":[{"iso":"eng"}],"has_accepted_license":"1","day":"04","citation":{"ieee":"T. Zisis <i>et al.</i>, “Sequential and switchable patterning for studying cellular processes under spatiotemporal control,” <i>ACS Applied Materials and Interfaces</i>, vol. 13, no. 30. American Chemical Society, pp. 35545–35560, 2021.","mla":"Zisis, Themistoklis, et al. “Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.” <i>ACS Applied Materials and Interfaces</i>, vol. 13, no. 30, American Chemical Society, 2021, pp. 35545–35560, doi:<a href=\"https://doi.org/10.1021/acsami.1c09850\">10.1021/acsami.1c09850</a>.","ista":"Zisis T, Schwarz J, Balles M, Kretschmer M, Nemethova M, Chait RP, Hauschild R, Lange J, Guet CC, Sixt MK, Zahler S. 2021. Sequential and switchable patterning for studying cellular processes under spatiotemporal control. ACS Applied Materials and Interfaces. 13(30), 35545–35560.","apa":"Zisis, T., Schwarz, J., Balles, M., Kretschmer, M., Nemethova, M., Chait, R. P., … Zahler, S. (2021). Sequential and switchable patterning for studying cellular processes under spatiotemporal control. <i>ACS Applied Materials and Interfaces</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsami.1c09850\">https://doi.org/10.1021/acsami.1c09850</a>","chicago":"Zisis, Themistoklis, Jan Schwarz, Miriam Balles, Maibritt Kretschmer, Maria Nemethova, Remy P Chait, Robert Hauschild, et al. “Sequential and Switchable Patterning for Studying Cellular Processes under Spatiotemporal Control.” <i>ACS Applied Materials and Interfaces</i>. American Chemical Society, 2021. <a href=\"https://doi.org/10.1021/acsami.1c09850\">https://doi.org/10.1021/acsami.1c09850</a>.","ama":"Zisis T, Schwarz J, Balles M, et al. Sequential and switchable patterning for studying cellular processes under spatiotemporal control. <i>ACS Applied Materials and Interfaces</i>. 2021;13(30):35545–35560. doi:<a href=\"https://doi.org/10.1021/acsami.1c09850\">10.1021/acsami.1c09850</a>","short":"T. Zisis, J. Schwarz, M. Balles, M. Kretschmer, M. Nemethova, R.P. Chait, R. Hauschild, J. Lange, C.C. Guet, M.K. Sixt, S. Zahler, ACS Applied Materials and Interfaces 13 (2021) 35545–35560."},"intvolume":"        13","ec_funded":1,"abstract":[{"text":"Attachment of adhesive molecules on cell culture surfaces to restrict cell adhesion to defined areas and shapes has been vital for the progress of in vitro research. In currently existing patterning methods, a combination of pattern properties such as stability, precision, specificity, high-throughput outcome, and spatiotemporal control is highly desirable but challenging to achieve. Here, we introduce a versatile and high-throughput covalent photoimmobilization technique, comprising a light-dose-dependent patterning step and a subsequent functionalization of the pattern via click chemistry. This two-step process is feasible on arbitrary surfaces and allows for generation of sustainable patterns and gradients. The method is validated in different biological systems by patterning adhesive ligands on cell-repellent surfaces, thereby constraining the growth and migration of cells to the designated areas. We then implement a sequential photopatterning approach by adding a second switchable patterning step, allowing for spatiotemporal control over two distinct surface patterns. As a proof of concept, we reconstruct the dynamics of the tip/stalk cell switch during angiogenesis. Our results show that the spatiotemporal control provided by our “sequential photopatterning” system is essential for mimicking dynamic biological processes and that our innovative approach has great potential for further applications in cell science.","lang":"eng"}],"status":"public","department":[{"_id":"MiSi"},{"_id":"GaTk"},{"_id":"Bio"},{"_id":"CaGu"}],"acknowledgement":"We would like to thank Charlott Leu for the production of our chromium wafers, Louise Ritter for her contribution of the IF stainings in Figure 4, Shokoufeh Teymouri for her help with the Bioinert coated slides, and finally Prof. Dr. Joachim Rädler for his valuable scientific guidance.","_id":"9822","month":"08","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_type":"original","publication_status":"published","oa":1,"title":"Sequential and switchable patterning for studying cellular processes under spatiotemporal control"},{"article_processing_charge":"No","pmid":1,"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2020.03.24.005835"}],"scopus_import":"1","publication_identifier":{"issn":["0960-9822"]},"date_updated":"2023-08-17T07:01:14Z","year":"2021","date_created":"2022-03-08T07:51:04Z","page":"2051-2064.e8","quality_controlled":"1","isi":1,"volume":31,"date_published":"2021-05-24T00:00:00Z","oa_version":"Preprint","external_id":{"isi":["000654652200002"],"pmid":["33711252"]},"author":[{"last_name":"Stahnke","first_name":"Stephanie","full_name":"Stahnke, Stephanie"},{"full_name":"Döring, Hermann","first_name":"Hermann","last_name":"Döring"},{"full_name":"Kusch, Charly","first_name":"Charly","last_name":"Kusch"},{"full_name":"de Gorter, David J.J.","first_name":"David J.J.","last_name":"de Gorter"},{"last_name":"Dütting","first_name":"Sebastian","full_name":"Dütting, Sebastian"},{"full_name":"Guledani, Aleks","first_name":"Aleks","last_name":"Guledani"},{"last_name":"Pleines","first_name":"Irina","full_name":"Pleines, Irina"},{"last_name":"Schnoor","full_name":"Schnoor, Michael","first_name":"Michael"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Geffers","first_name":"Robert","full_name":"Geffers, Robert"},{"last_name":"Rohde","full_name":"Rohde, Manfred","first_name":"Manfred"},{"last_name":"Müsken","first_name":"Mathias","full_name":"Müsken, Mathias"},{"first_name":"Frieda","full_name":"Kage, Frieda","last_name":"Kage"},{"first_name":"Anika","full_name":"Steffen, Anika","last_name":"Steffen"},{"full_name":"Faix, Jan","first_name":"Jan","last_name":"Faix"},{"last_name":"Nieswandt","first_name":"Bernhard","full_name":"Nieswandt, Bernhard"},{"full_name":"Rottner, Klemens","first_name":"Klemens","last_name":"Rottner"},{"last_name":"Stradal","first_name":"Theresia E.B.","full_name":"Stradal, Theresia E.B."}],"publication":"Current Biology","issue":"10","publisher":"Elsevier","doi":"10.1016/j.cub.2021.02.043","type":"journal_article","language":[{"iso":"eng"}],"day":"24","citation":{"ista":"Stahnke S, Döring H, Kusch C, de Gorter DJJ, Dütting S, Guledani A, Pleines I, Schnoor M, Sixt MK, Geffers R, Rohde M, Müsken M, Kage F, Steffen A, Faix J, Nieswandt B, Rottner K, Stradal TEB. 2021. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. Current Biology. 31(10), 2051–2064.e8.","chicago":"Stahnke, Stephanie, Hermann Döring, Charly Kusch, David J.J. de Gorter, Sebastian Dütting, Aleks Guledani, Irina Pleines, et al. “Loss of Hem1 Disrupts Macrophage Function and Impacts Migration, Phagocytosis, and Integrin-Mediated Adhesion.” <i>Current Biology</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">https://doi.org/10.1016/j.cub.2021.02.043</a>.","apa":"Stahnke, S., Döring, H., Kusch, C., de Gorter, D. J. J., Dütting, S., Guledani, A., … Stradal, T. E. B. (2021). Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. <i>Current Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">https://doi.org/10.1016/j.cub.2021.02.043</a>","mla":"Stahnke, Stephanie, et al. “Loss of Hem1 Disrupts Macrophage Function and Impacts Migration, Phagocytosis, and Integrin-Mediated Adhesion.” <i>Current Biology</i>, vol. 31, no. 10, Elsevier, 2021, p. 2051–2064.e8, doi:<a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">10.1016/j.cub.2021.02.043</a>.","ieee":"S. Stahnke <i>et al.</i>, “Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion,” <i>Current Biology</i>, vol. 31, no. 10. Elsevier, p. 2051–2064.e8, 2021.","short":"S. Stahnke, H. Döring, C. Kusch, D.J.J. de Gorter, S. Dütting, A. Guledani, I. Pleines, M. Schnoor, M.K. Sixt, R. Geffers, M. Rohde, M. Müsken, F. Kage, A. Steffen, J. Faix, B. Nieswandt, K. Rottner, T.E.B. Stradal, Current Biology 31 (2021) 2051–2064.e8.","ama":"Stahnke S, Döring H, Kusch C, et al. Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion. <i>Current Biology</i>. 2021;31(10):2051-2064.e8. doi:<a href=\"https://doi.org/10.1016/j.cub.2021.02.043\">10.1016/j.cub.2021.02.043</a>"},"intvolume":"        31","keyword":["General Agricultural and Biological Sciences","General Biochemistry","Genetics and Molecular Biology"],"abstract":[{"text":"Hematopoietic-specific protein 1 (Hem1) is an essential subunit of the WAVE regulatory complex (WRC) in immune cells. WRC is crucial for Arp2/3 complex activation and the protrusion of branched actin filament networks. Moreover, Hem1 loss of function in immune cells causes autoimmune diseases in humans. Here, we show that genetic removal of Hem1 in macrophages diminishes frequency and efficacy of phagocytosis as well as phagocytic cup formation in addition to defects in lamellipodial protrusion and migration. Moreover, Hem1-null macrophages displayed strong defects in cell adhesion despite unaltered podosome formation and concomitant extracellular matrix degradation. Specifically, dynamics of both adhesion and de-adhesion as well as concomitant phosphorylation of paxillin and focal adhesion kinase (FAK) were significantly compromised. Accordingly, disruption of WRC function in non-hematopoietic cells coincided with both defects in adhesion turnover and altered FAK and paxillin phosphorylation. Consistently, platelets exhibited reduced adhesion and diminished integrin αIIbβ3 activation upon WRC removal. Interestingly, adhesion phenotypes, but not lamellipodia formation, were partially rescued by small molecule activation of FAK. A full rescue of the phenotype, including lamellipodia formation, required not only the presence of WRCs but also their binding to and activation by Rac. Collectively, our results uncover that WRC impacts on integrin-dependent processes in a FAK-dependent manner, controlling formation and dismantling of adhesions, relevant for properly grabbing onto extracellular surfaces and particles during cell edge expansion, like in migration or phagocytosis.","lang":"eng"}],"status":"public","department":[{"_id":"MiSi"}],"acknowledgement":"We are grateful to Silvia Prettin, Ina Schleicher, and Petra Hagendorff for expert technical assistance; David Dettbarn for animal keeping and breeding; and Lothar Gröbe and Maria Höxter for cell sorting. We also thank Werner Tegge for peptides and Giorgio Scita for antibodies. This work was supported, in part, by the Deutsche Forschungsgemeinschaft (DFG), Priority Programm SPP1150 (to T.E.B.S., K.R., and M. Sixt), and by DFG grant GRK2223/1 (to K.R.). T.E.B.S. acknowledges support by the Helmholtz Society through HGF impulse fund W2/W3-066 and M. Schnoor by the Mexican Council for Science and Technology (CONACyT, 284292 ), Fund SEP-Cinvestav ( 108 ), and the Royal Society, UK (Newton Advanced Fellowship, NAF/R1/180017 ).","month":"05","_id":"10834","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","article_type":"original","publication_status":"published","oa":1,"title":"Loss of Hem1 disrupts macrophage function and impacts migration, phagocytosis, and integrin-mediated adhesion"},{"volume":11,"isi":1,"file":[{"relation":"main_file","checksum":"485b7b6cf30198ba0ce126491a28f125","file_size":7035340,"creator":"dernst","file_name":"2020_NatureComm_Nicolai.pdf","file_id":"8798","date_created":"2020-11-23T13:29:49Z","content_type":"application/pdf","date_updated":"2020-11-23T13:29:49Z","success":1,"access_level":"open_access"}],"date_updated":"2023-08-22T13:26:26Z","project":[{"call_identifier":"H2020","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"}],"quality_controlled":"1","date_created":"2020-11-22T23:01:23Z","year":"2020","scopus_import":"1","pmid":1,"publication_identifier":{"eissn":["20411723"]},"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"file_date_updated":"2020-11-23T13:29:49Z","type":"journal_article","language":[{"iso":"eng"}],"publisher":"Springer Nature","doi":"10.1038/s41467-020-19515-0","ddc":["570"],"author":[{"last_name":"Nicolai","full_name":"Nicolai, Leo","first_name":"Leo"},{"last_name":"Schiefelbein","full_name":"Schiefelbein, Karin","first_name":"Karin"},{"full_name":"Lipsky, Silvia","first_name":"Silvia","last_name":"Lipsky"},{"first_name":"Alexander","full_name":"Leunig, Alexander","last_name":"Leunig"},{"first_name":"Marie","full_name":"Hoffknecht, Marie","last_name":"Hoffknecht"},{"full_name":"Pekayvaz, Kami","first_name":"Kami","last_name":"Pekayvaz"},{"last_name":"Raude","full_name":"Raude, Ben","first_name":"Ben"},{"first_name":"Charlotte","full_name":"Marx, Charlotte","last_name":"Marx"},{"full_name":"Ehrlich, Andreas","first_name":"Andreas","last_name":"Ehrlich"},{"full_name":"Pircher, Joachim","first_name":"Joachim","last_name":"Pircher"},{"full_name":"Zhang, Zhe","first_name":"Zhe","last_name":"Zhang"},{"last_name":"Saleh","first_name":"Inas","full_name":"Saleh, Inas"},{"last_name":"Marel","full_name":"Marel, Anna-Kristina","first_name":"Anna-Kristina"},{"last_name":"Löf","full_name":"Löf, Achim","first_name":"Achim"},{"last_name":"Petzold","full_name":"Petzold, Tobias","first_name":"Tobias"},{"last_name":"Lorenz","full_name":"Lorenz, Michael","first_name":"Michael"},{"last_name":"Stark","first_name":"Konstantin","full_name":"Stark, Konstantin"},{"last_name":"Pick","first_name":"Robert","full_name":"Pick, Robert"},{"full_name":"Rosenberger, Gerhild","first_name":"Gerhild","last_name":"Rosenberger"},{"full_name":"Weckbach, Ludwig","first_name":"Ludwig","last_name":"Weckbach"},{"first_name":"Bernd","full_name":"Uhl, Bernd","last_name":"Uhl"},{"first_name":"Sheng","full_name":"Xia, Sheng","last_name":"Xia"},{"full_name":"Reichel, Christoph Andreas","first_name":"Christoph Andreas","last_name":"Reichel"},{"last_name":"Walzog","first_name":"Barbara","full_name":"Walzog, Barbara"},{"last_name":"Schulz","first_name":"Christian","full_name":"Schulz, Christian"},{"last_name":"Zheden","full_name":"Zheden, Vanessa","first_name":"Vanessa","orcid":"0000-0002-9438-4783","id":"39C5A68A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Bender","full_name":"Bender, Markus","first_name":"Markus"},{"last_name":"Li","first_name":"Rong","full_name":"Li, Rong"},{"last_name":"Massberg","first_name":"Steffen","full_name":"Massberg, Steffen"},{"last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R","orcid":"0000-0001-6120-3723","id":"397A88EE-F248-11E8-B48F-1D18A9856A87"}],"publication":"Nature Communications","date_published":"2020-11-13T00:00:00Z","oa_version":"Published Version","external_id":{"isi":["000594648000014"],"pmid":["33188196"]},"abstract":[{"lang":"eng","text":"Breakdown of vascular barriers is a major complication of inflammatory diseases. Anucleate platelets form blood-clots during thrombosis, but also play a crucial role in inflammation. While spatio-temporal dynamics of clot formation are well characterized, the cell-biological mechanisms of platelet recruitment to inflammatory micro-environments remain incompletely understood. Here we identify Arp2/3-dependent lamellipodia formation as a prominent morphological feature of immune-responsive platelets. Platelets use lamellipodia to scan for fibrin(ogen) deposited on the inflamed vasculature and to directionally spread, to polarize and to govern haptotactic migration along gradients of the adhesive ligand. Platelet-specific abrogation of Arp2/3 interferes with haptotactic repositioning of platelets to microlesions, thus impairing vascular sealing and provoking inflammatory microbleeding. During infection, haptotaxis promotes capture of bacteria and prevents hematogenic dissemination, rendering platelets gate-keepers of the inflamed microvasculature. Consequently, these findings identify haptotaxis as a key effector function of immune-responsive platelets."}],"article_number":"5778","status":"public","ec_funded":1,"intvolume":"        11","citation":{"ama":"Nicolai L, Schiefelbein K, Lipsky S, et al. Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. 2020;11. doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>","short":"L. Nicolai, K. Schiefelbein, S. Lipsky, A. Leunig, M. Hoffknecht, K. Pekayvaz, B. Raude, C. Marx, A. Ehrlich, J. Pircher, Z. Zhang, I. Saleh, A.-K. Marel, A. Löf, T. Petzold, M. Lorenz, K. Stark, R. Pick, G. Rosenberger, L. Weckbach, B. Uhl, S. Xia, C.A. Reichel, B. Walzog, C. Schulz, V. Zheden, M. Bender, R. Li, S. Massberg, F.R. Gärtner, Nature Communications 11 (2020).","mla":"Nicolai, Leo, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>, vol. 11, 5778, Springer Nature, 2020, doi:<a href=\"https://doi.org/10.1038/s41467-020-19515-0\">10.1038/s41467-020-19515-0</a>.","ieee":"L. Nicolai <i>et al.</i>, “Vascular surveillance by haptotactic blood platelets in inflammation and infection,” <i>Nature Communications</i>, vol. 11. Springer Nature, 2020.","apa":"Nicolai, L., Schiefelbein, K., Lipsky, S., Leunig, A., Hoffknecht, M., Pekayvaz, K., … Gärtner, F. R. (2020). Vascular surveillance by haptotactic blood platelets in inflammation and infection. <i>Nature Communications</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>","ista":"Nicolai L, Schiefelbein K, Lipsky S, Leunig A, Hoffknecht M, Pekayvaz K, Raude B, Marx C, Ehrlich A, Pircher J, Zhang Z, Saleh I, Marel A-K, Löf A, Petzold T, Lorenz M, Stark K, Pick R, Rosenberger G, Weckbach L, Uhl B, Xia S, Reichel CA, Walzog B, Schulz C, Zheden V, Bender M, Li R, Massberg S, Gärtner FR. 2020. Vascular surveillance by haptotactic blood platelets in inflammation and infection. Nature Communications. 11, 5778.","chicago":"Nicolai, Leo, Karin Schiefelbein, Silvia Lipsky, Alexander Leunig, Marie Hoffknecht, Kami Pekayvaz, Ben Raude, et al. “Vascular Surveillance by Haptotactic Blood Platelets in Inflammation and Infection.” <i>Nature Communications</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41467-020-19515-0\">https://doi.org/10.1038/s41467-020-19515-0</a>."},"has_accepted_license":"1","day":"13","article_type":"original","related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-022-31310-7","relation":"erratum"}]},"title":"Vascular surveillance by haptotactic blood platelets in inflammation and infection","oa":1,"publication_status":"published","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"8787","month":"11","department":[{"_id":"MiSi"},{"_id":"EM-Fac"}],"acknowledgement":"We thank Sebastian Helmer, Nicole Blount, Christine Mann, and Beate Jantz for technical assistance; Hellen Ishikawa-Ankerhold for help and advice; Michael Sixt for critical\r\ndiscussions. This study was supported by the DFG SFB 914 (S.M. [B02 and Z01], K.Sch.\r\n[B02], B.W. [A02 and Z03], C.A.R. [B03], C.S. [A10], J.P. [Gerok position]), the DFG\r\nSFB 1123 (S.M. [B06]), the DFG FOR 2033 (S.M. and F.G.), the German Center for\r\nCardiovascular Research (DZHK) (Clinician Scientist Program [L.N.], MHA 1.4VD\r\n[S.M.], Postdoc Start-up Grant, 81×3600213 [F.G.]), FP7 program (project 260309,\r\nPRESTIGE [S.M.]), FöFoLe project 1015/1009 (L.N.), FöFoLe project 947 (F.G.), the\r\nFriedrich-Baur-Stiftung project 41/16 (F.G.), and LMUexcellence NFF (F.G.). This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no.\r\n833440) (S.M.). F.G. received funding from the European Union’s Horizon 2020 research\r\nand innovation program under the Marie Skłodowska-Curie grant agreement no.\r\n747687."},{"_id":"7234","month":"02","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","oa":1,"title":"Partial loss of actin nucleator actin-related protein 2/3 activity triggers blebbing in primary T lymphocytes","article_type":"original","department":[{"_id":"MiSi"}],"status":"public","abstract":[{"text":"T lymphocytes utilize amoeboid migration to navigate effectively within complex microenvironments. The precise rearrangement of the actin cytoskeleton required for cellular forward propulsion is mediated by actin regulators, including the actin‐related protein 2/3 (Arp2/3) complex, a macromolecular machine that nucleates branched actin filaments at the leading edge. The consequences of modulating Arp2/3 activity on the biophysical properties of the actomyosin cortex and downstream T cell function are incompletely understood. We report that even a moderate decrease of Arp3 levels in T cells profoundly affects actin cortex integrity. Reduction in total F‐actin content leads to reduced cortical tension and disrupted lamellipodia formation. Instead, in Arp3‐knockdown cells, the motility mode is dominated by blebbing migration characterized by transient, balloon‐like protrusions at the leading edge. Although this migration mode seems to be compatible with interstitial migration in three‐dimensional environments, diminished locomotion kinetics and impaired cytotoxicity interfere with optimal T cell function. These findings define the importance of finely tuned, Arp2/3‐dependent mechanophysical membrane integrity in cytotoxic effector T lymphocyte activities.","lang":"eng"}],"day":"01","has_accepted_license":"1","citation":{"chicago":"Obeidy, Peyman, Lining A. Ju, Stefan H. Oehlers, Nursafwana S. Zulkhernain, Quintin Lee, Jorge L. Galeano Niño, Rain Y.Q. Kwan, et al. “Partial Loss of Actin Nucleator Actin-Related Protein 2/3 Activity Triggers Blebbing in Primary T Lymphocytes.” <i>Immunology and Cell Biology</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/imcb.12304\">https://doi.org/10.1111/imcb.12304</a>.","ista":"Obeidy P, Ju LA, Oehlers SH, Zulkhernain NS, Lee Q, Galeano Niño JL, Kwan RYQ, Tikoo S, Cavanagh LL, Mrass P, Cook AJL, Jackson SP, Biro M, Roediger B, Sixt MK, Weninger W. 2020. Partial loss of actin nucleator actin-related protein 2/3 activity triggers blebbing in primary T lymphocytes. Immunology and Cell Biology. 98(2), 93–113.","apa":"Obeidy, P., Ju, L. A., Oehlers, S. H., Zulkhernain, N. S., Lee, Q., Galeano Niño, J. L., … Weninger, W. (2020). Partial loss of actin nucleator actin-related protein 2/3 activity triggers blebbing in primary T lymphocytes. <i>Immunology and Cell Biology</i>. Wiley. <a href=\"https://doi.org/10.1111/imcb.12304\">https://doi.org/10.1111/imcb.12304</a>","ieee":"P. Obeidy <i>et al.</i>, “Partial loss of actin nucleator actin-related protein 2/3 activity triggers blebbing in primary T lymphocytes,” <i>Immunology and Cell Biology</i>, vol. 98, no. 2. Wiley, pp. 93–113, 2020.","mla":"Obeidy, Peyman, et al. “Partial Loss of Actin Nucleator Actin-Related Protein 2/3 Activity Triggers Blebbing in Primary T Lymphocytes.” <i>Immunology and Cell Biology</i>, vol. 98, no. 2, Wiley, 2020, pp. 93–113, doi:<a href=\"https://doi.org/10.1111/imcb.12304\">10.1111/imcb.12304</a>.","short":"P. Obeidy, L.A. Ju, S.H. Oehlers, N.S. Zulkhernain, Q. Lee, J.L. Galeano Niño, R.Y.Q. Kwan, S. Tikoo, L.L. Cavanagh, P. Mrass, A.J.L. Cook, S.P. Jackson, M. Biro, B. Roediger, M.K. Sixt, W. Weninger, Immunology and Cell Biology 98 (2020) 93–113.","ama":"Obeidy P, Ju LA, Oehlers SH, et al. Partial loss of actin nucleator actin-related protein 2/3 activity triggers blebbing in primary T lymphocytes. <i>Immunology and Cell Biology</i>. 2020;98(2):93-113. doi:<a href=\"https://doi.org/10.1111/imcb.12304\">10.1111/imcb.12304</a>"},"intvolume":"        98","doi":"10.1111/imcb.12304","publisher":"Wiley","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2020-11-19T11:22:33Z","oa_version":"Published Version","external_id":{"isi":["000503885600001"],"pmid":["31698518"]},"date_published":"2020-02-01T00:00:00Z","issue":"2","publication":"Immunology and Cell Biology","author":[{"first_name":"Peyman","full_name":"Obeidy, Peyman","last_name":"Obeidy"},{"full_name":"Ju, Lining A.","first_name":"Lining A.","last_name":"Ju"},{"last_name":"Oehlers","first_name":"Stefan H.","full_name":"Oehlers, Stefan H."},{"last_name":"Zulkhernain","full_name":"Zulkhernain, Nursafwana S.","first_name":"Nursafwana S."},{"first_name":"Quintin","full_name":"Lee, Quintin","last_name":"Lee"},{"last_name":"Galeano Niño","full_name":"Galeano Niño, Jorge L.","first_name":"Jorge L."},{"last_name":"Kwan","first_name":"Rain Y.Q.","full_name":"Kwan, Rain Y.Q."},{"full_name":"Tikoo, Shweta","first_name":"Shweta","last_name":"Tikoo"},{"full_name":"Cavanagh, Lois L.","first_name":"Lois L.","last_name":"Cavanagh"},{"last_name":"Mrass","first_name":"Paulus","full_name":"Mrass, Paulus"},{"first_name":"Adam J.L.","full_name":"Cook, Adam J.L.","last_name":"Cook"},{"first_name":"Shaun P.","full_name":"Jackson, Shaun P.","last_name":"Jackson"},{"first_name":"Maté","full_name":"Biro, Maté","last_name":"Biro"},{"full_name":"Roediger, Ben","first_name":"Ben","last_name":"Roediger"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"},{"last_name":"Weninger","first_name":"Wolfgang","full_name":"Weninger, Wolfgang"}],"ddc":["570"],"year":"2020","date_created":"2020-01-05T23:00:48Z","page":"93-113","quality_controlled":"1","date_updated":"2023-08-17T14:21:12Z","isi":1,"file":[{"file_name":"2020_ImmunologyCellBio_Obeidy.pdf","content_type":"application/pdf","date_created":"2020-11-19T11:22:33Z","file_id":"8775","date_updated":"2020-11-19T11:22:33Z","access_level":"open_access","success":1,"relation":"main_file","checksum":"c389477b4b52172ef76afff8a06c6775","file_size":8569945,"creator":"dernst"}],"volume":98,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"No","publication_identifier":{"issn":["08189641"],"eissn":["14401711"]},"pmid":1,"scopus_import":"1"},{"acknowledgement":"This work has been supported by the Vienna Science and Technology Fund, Grant no. LS13-029. G.J. and C.S. also acknowledge support by the Austrian Science Fund, Grants no. W1245, F 65, and W1261, as well as by the Fondation Sciences Mathématiques de Paris, and by Paris-Sciences-et-Lettres.","department":[{"_id":"MiSi"}],"_id":"7623","month":"03","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","title":"Modeling adhesion-independent cell migration","oa":1,"article_type":"original","day":"18","intvolume":"        30","citation":{"ama":"Jankowiak G, Peurichard D, Reversat A, Schmeiser C, Sixt MK. Modeling adhesion-independent cell migration. <i>Mathematical Models and Methods in Applied Sciences</i>. 2020;30(3):513-537. doi:<a href=\"https://doi.org/10.1142/S021820252050013X\">10.1142/S021820252050013X</a>","short":"G. Jankowiak, D. Peurichard, A. Reversat, C. Schmeiser, M.K. Sixt, Mathematical Models and Methods in Applied Sciences 30 (2020) 513–537.","ieee":"G. Jankowiak, D. Peurichard, A. Reversat, C. Schmeiser, and M. K. Sixt, “Modeling adhesion-independent cell migration,” <i>Mathematical Models and Methods in Applied Sciences</i>, vol. 30, no. 3. World Scientific, pp. 513–537, 2020.","mla":"Jankowiak, Gaspard, et al. “Modeling Adhesion-Independent Cell Migration.” <i>Mathematical Models and Methods in Applied Sciences</i>, vol. 30, no. 3, World Scientific, 2020, pp. 513–37, doi:<a href=\"https://doi.org/10.1142/S021820252050013X\">10.1142/S021820252050013X</a>.","chicago":"Jankowiak, Gaspard, Diane Peurichard, Anne Reversat, Christian Schmeiser, and Michael K Sixt. “Modeling Adhesion-Independent Cell Migration.” <i>Mathematical Models and Methods in Applied Sciences</i>. World Scientific, 2020. <a href=\"https://doi.org/10.1142/S021820252050013X\">https://doi.org/10.1142/S021820252050013X</a>.","ista":"Jankowiak G, Peurichard D, Reversat A, Schmeiser C, Sixt MK. 2020. Modeling adhesion-independent cell migration. Mathematical Models and Methods in Applied Sciences. 30(3), 513–537.","apa":"Jankowiak, G., Peurichard, D., Reversat, A., Schmeiser, C., &#38; Sixt, M. K. (2020). Modeling adhesion-independent cell migration. <i>Mathematical Models and Methods in Applied Sciences</i>. World Scientific. <a href=\"https://doi.org/10.1142/S021820252050013X\">https://doi.org/10.1142/S021820252050013X</a>"},"status":"public","abstract":[{"text":"A two-dimensional mathematical model for cells migrating without adhesion capabilities is presented and analyzed. Cells are represented by their cortex, which is modeled as an elastic curve, subject to an internal pressure force. Net polymerization or depolymerization in the cortex is modeled via local addition or removal of material, driving a cortical flow. The model takes the form of a fully nonlinear degenerate parabolic system. An existence analysis is carried out by adapting ideas from the theory of gradient flows. Numerical simulations show that these simple rules can account for the behavior observed in experiments, suggesting a possible mechanical mechanism for adhesion-independent motility.","lang":"eng"}],"external_id":{"arxiv":["1903.09426"],"isi":["000525349900003"]},"oa_version":"Preprint","date_published":"2020-03-18T00:00:00Z","issue":"3","publication":"Mathematical Models and Methods in Applied Sciences","author":[{"last_name":"Jankowiak","first_name":"Gaspard","full_name":"Jankowiak, Gaspard"},{"last_name":"Peurichard","full_name":"Peurichard, Diane","first_name":"Diane"},{"orcid":"0000-0003-0666-8928","id":"35B76592-F248-11E8-B48F-1D18A9856A87","first_name":"Anne","full_name":"Reversat, Anne","last_name":"Reversat"},{"last_name":"Schmeiser","full_name":"Schmeiser, Christian","first_name":"Christian"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K"}],"doi":"10.1142/S021820252050013X","publisher":"World Scientific","language":[{"iso":"eng"}],"arxiv":1,"type":"journal_article","article_processing_charge":"No","publication_identifier":{"issn":["02182025"]},"main_file_link":[{"url":"https://arxiv.org/abs/1903.09426","open_access":"1"}],"scopus_import":"1","year":"2020","date_created":"2020-03-31T11:25:05Z","page":"513-537","quality_controlled":"1","project":[{"_id":"25AD6156-B435-11E9-9278-68D0E5697425","name":"Modeling of Polarization and Motility of Leukocytes in Three-Dimensional Environments","grant_number":"LS13-029"}],"date_updated":"2023-08-18T10:18:56Z","isi":1,"volume":30},{"doi":"10.1083/jcb.201907154","publisher":"Rockefeller University Press","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2020-11-24T13:25:13Z","oa_version":"Published Version","external_id":{"isi":["000538141100020"],"pmid":["32379884"]},"date_published":"2020-06-01T00:00:00Z","publication":"The Journal of Cell Biology","issue":"6","author":[{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","last_name":"Kopf","first_name":"Aglaja","full_name":"Kopf, Aglaja"},{"orcid":"0000-0003-2856-3369","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","full_name":"Renkawitz, Jörg","first_name":"Jörg","last_name":"Renkawitz"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert","full_name":"Hauschild, Robert","last_name":"Hauschild"},{"full_name":"Girkontaite, Irute","first_name":"Irute","last_name":"Girkontaite"},{"full_name":"Tedford, Kerry","first_name":"Kerry","last_name":"Tedford"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609","first_name":"Jack","full_name":"Merrin, Jack","last_name":"Merrin"},{"full_name":"Thorn-Seshold, Oliver","first_name":"Oliver","last_name":"Thorn-Seshold"},{"last_name":"Trauner","first_name":"Dirk","full_name":"Trauner, Dirk","id":"E8F27F48-3EBA-11E9-92A1-B709E6697425"},{"last_name":"Häcker","first_name":"Hans","full_name":"Häcker, Hans"},{"first_name":"Klaus Dieter","full_name":"Fischer, Klaus Dieter","last_name":"Fischer"},{"orcid":"0000-0001-6165-5738","id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","last_name":"Kiermaier","first_name":"Eva","full_name":"Kiermaier, Eva"},{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"ddc":["570"],"year":"2020","date_created":"2020-05-24T22:00:56Z","quality_controlled":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"PreCl"}],"project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"281556","call_identifier":"FP7"},{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373","call_identifier":"H2020"},{"_id":"26018E70-B435-11E9-9278-68D0E5697425","name":"Mechanical adaptation of lamellipodial actin","grant_number":"P29911","call_identifier":"FWF"},{"_id":"252C3B08-B435-11E9-9278-68D0E5697425","name":"Nano-Analytics of Cellular Systems","grant_number":"W 1250-B20","call_identifier":"FWF"},{"name":"International IST Postdoc Fellowship Programme","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"_id":"25A48D24-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 1396-2014","name":"Molecular and system level view of immune cell migration"}],"date_updated":"2023-08-21T06:28:17Z","isi":1,"file":[{"checksum":"cb0b9c77842ae1214caade7b77e4d82d","relation":"main_file","creator":"dernst","file_size":7536712,"file_name":"2020_JCellBiol_Kopf.pdf","success":1,"access_level":"open_access","date_updated":"2020-11-24T13:25:13Z","file_id":"8801","content_type":"application/pdf","date_created":"2020-11-24T13:25:13Z"}],"volume":219,"article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"publication_identifier":{"eissn":["1540-8140"]},"pmid":1,"scopus_import":"1","_id":"7875","month":"06","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","publication_status":"published","title":"Microtubules control cellular shape and coherence in amoeboid migrating cells","oa":1,"article_type":"original","acknowledgement":"The authors thank the Scientific Service Units (Life Sciences, Bioimaging, Preclinical) of the Institute of Science and Technology Austria for excellent support. This work was funded by the European Research Council (ERC StG 281556 and CoG 724373), two grants from the Austrian\r\nScience Fund (FWF; P29911 and DK Nanocell W1250-B20 to M. Sixt) and by the German Research Foundation (DFG SFB1032 project B09) to O. Thorn-Seshold and D. Trauner. J. Renkawitz was supported by ISTFELLOW funding from the People Program (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) under the Research Executive Agency grant agreement (291734) and a European Molecular Biology Organization long-term fellowship (ALTF 1396-2014) co-funded by the European Commission (LTFCOFUND2013, GA-2013-609409), E. Kiermaier by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy—EXC 2151—390873048, and H. Hacker by the American Lebanese Syrian Associated ¨Charities. K.-D. Fischer was supported by the Analysis, Imaging and Modelling of Neuronal and Inflammatory Processes graduate school funded by the Ministry of Economics, Science, and Digitisation of the State Saxony-Anhalt and by the European Funds for Social and Regional Development.","department":[{"_id":"MiSi"},{"_id":"Bio"},{"_id":"NanoFab"}],"ec_funded":1,"status":"public","article_number":"e201907154","abstract":[{"lang":"eng","text":"Cells navigating through complex tissues face a fundamental challenge: while multiple protrusions explore different paths, the cell needs to avoid entanglement. How a cell surveys and then corrects its own shape is poorly understood. Here, we demonstrate that spatially distinct microtubule dynamics regulate amoeboid cell migration by locally promoting the retraction of protrusions. In migrating dendritic cells, local microtubule depolymerization within protrusions remote from the microtubule organizing center triggers actomyosin contractility controlled by RhoA and its exchange factor Lfc. Depletion of Lfc leads to aberrant myosin localization, thereby causing two effects that rate-limit locomotion: (1) impaired cell edge coordination during path finding and (2) defective adhesion resolution. Compromised shape control is particularly hindering in geometrically complex microenvironments, where it leads to entanglement and ultimately fragmentation of the cell body. We thus demonstrate that microtubules can act as a proprioceptive device: they sense cell shape and control actomyosin retraction to sustain cellular coherence."}],"day":"01","has_accepted_license":"1","citation":{"ista":"Kopf A, Renkawitz J, Hauschild R, Girkontaite I, Tedford K, Merrin J, Thorn-Seshold O, Trauner D, Häcker H, Fischer KD, Kiermaier E, Sixt MK. 2020. Microtubules control cellular shape and coherence in amoeboid migrating cells. The Journal of Cell Biology. 219(6), e201907154.","apa":"Kopf, A., Renkawitz, J., Hauschild, R., Girkontaite, I., Tedford, K., Merrin, J., … Sixt, M. K. (2020). Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>","chicago":"Kopf, Aglaja, Jörg Renkawitz, Robert Hauschild, Irute Girkontaite, Kerry Tedford, Jack Merrin, Oliver Thorn-Seshold, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>. Rockefeller University Press, 2020. <a href=\"https://doi.org/10.1083/jcb.201907154\">https://doi.org/10.1083/jcb.201907154</a>.","ieee":"A. Kopf <i>et al.</i>, “Microtubules control cellular shape and coherence in amoeboid migrating cells,” <i>The Journal of Cell Biology</i>, vol. 219, no. 6. Rockefeller University Press, 2020.","mla":"Kopf, Aglaja, et al. “Microtubules Control Cellular Shape and Coherence in Amoeboid Migrating Cells.” <i>The Journal of Cell Biology</i>, vol. 219, no. 6, e201907154, Rockefeller University Press, 2020, doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>.","short":"A. Kopf, J. Renkawitz, R. Hauschild, I. Girkontaite, K. Tedford, J. Merrin, O. Thorn-Seshold, D. Trauner, H. Häcker, K.D. Fischer, E. Kiermaier, M.K. Sixt, The Journal of Cell Biology 219 (2020).","ama":"Kopf A, Renkawitz J, Hauschild R, et al. Microtubules control cellular shape and coherence in amoeboid migrating cells. <i>The Journal of Cell Biology</i>. 2020;219(6). doi:<a href=\"https://doi.org/10.1083/jcb.201907154\">10.1083/jcb.201907154</a>"},"intvolume":"       219"},{"type":"journal_article","language":[{"iso":"eng"}],"publisher":"Elsevier","doi":"10.1016/j.immuni.2020.04.020","author":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K"},{"last_name":"Lämmermann","full_name":"Lämmermann, Tim","first_name":"Tim"}],"issue":"5","publication":"Immunity","date_published":"2020-05-19T00:00:00Z","external_id":{"isi":["000535371100002"]},"oa_version":"Published Version","volume":52,"isi":1,"date_updated":"2023-08-21T06:27:18Z","page":"721-723","quality_controlled":"1","date_created":"2020-05-24T22:00:57Z","year":"2020","scopus_import":"1","main_file_link":[{"url":"https://pure.mpg.de/pubman/item/item_3265599_2/component/file_3265620/Sixt%20et%20al..pdf","open_access":"1"}],"publication_identifier":{"issn":["10747613"],"eissn":["10974180"]},"article_processing_charge":"No","article_type":"original","title":"T cells: Bridge-and-channel commute to the white pulp","oa":1,"publication_status":"published","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"05","_id":"7876","department":[{"_id":"MiSi"}],"abstract":[{"lang":"eng","text":"In contrast to lymph nodes, the lymphoid regions of the spleen—the white pulp—are located deep within the organ, yielding the trafficking paths of T cells in the white pulp largely invisible. In an intravital microscopy tour de force reported in this issue of Immunity, Chauveau et al. show that T cells perform unidirectional, perivascular migration through the enigmatic marginal zone bridging channels. "}],"status":"public","citation":{"short":"M.K. Sixt, T. Lämmermann, Immunity 52 (2020) 721–723.","ama":"Sixt MK, Lämmermann T. T cells: Bridge-and-channel commute to the white pulp. <i>Immunity</i>. 2020;52(5):721-723. doi:<a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">10.1016/j.immuni.2020.04.020</a>","chicago":"Sixt, Michael K, and Tim Lämmermann. “T Cells: Bridge-and-Channel Commute to the White Pulp.” <i>Immunity</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">https://doi.org/10.1016/j.immuni.2020.04.020</a>.","apa":"Sixt, M. K., &#38; Lämmermann, T. (2020). T cells: Bridge-and-channel commute to the white pulp. <i>Immunity</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">https://doi.org/10.1016/j.immuni.2020.04.020</a>","ista":"Sixt MK, Lämmermann T. 2020. T cells: Bridge-and-channel commute to the white pulp. Immunity. 52(5), 721–723.","ieee":"M. K. Sixt and T. Lämmermann, “T cells: Bridge-and-channel commute to the white pulp,” <i>Immunity</i>, vol. 52, no. 5. Elsevier, pp. 721–723, 2020.","mla":"Sixt, Michael K., and Tim Lämmermann. “T Cells: Bridge-and-Channel Commute to the White Pulp.” <i>Immunity</i>, vol. 52, no. 5, Elsevier, 2020, pp. 721–23, doi:<a href=\"https://doi.org/10.1016/j.immuni.2020.04.020\">10.1016/j.immuni.2020.04.020</a>."},"intvolume":"        52","day":"19"},{"volume":582,"isi":1,"quality_controlled":"1","page":"582–585","year":"2020","date_created":"2020-05-24T22:01:01Z","date_updated":"2024-03-25T23:30:12Z","project":[{"name":"Cytoskeletal force generation and force transduction of migrating leukocytes","grant_number":"281556","_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7"},{"call_identifier":"H2020","grant_number":"724373","name":"Cellular navigation along spatial gradients","_id":"25FE9508-B435-11E9-9278-68D0E5697425"},{"grant_number":"P29911","name":"Mechanical adaptation of lamellipodial actin","_id":"26018E70-B435-11E9-9278-68D0E5697425","call_identifier":"FWF"},{"grant_number":"747687","name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","_id":"260AA4E2-B435-11E9-9278-68D0E5697425","call_identifier":"H2020"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"M-Shop"}],"publication_identifier":{"eissn":["14764687"],"issn":["00280836"]},"scopus_import":"1","article_processing_charge":"No","language":[{"iso":"eng"}],"type":"journal_article","doi":"10.1038/s41586-020-2283-z","publisher":"Springer Nature","publication":"Nature","author":[{"first_name":"Anne","full_name":"Reversat, Anne","last_name":"Reversat","id":"35B76592-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-0666-8928"},{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6120-3723","last_name":"Gärtner","full_name":"Gärtner, Florian R","first_name":"Florian R"},{"full_name":"Merrin, Jack","first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5145-4609"},{"id":"489E3F00-F248-11E8-B48F-1D18A9856A87","full_name":"Stopp, Julian A","first_name":"Julian A","last_name":"Stopp"},{"id":"4323B49C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1671-393X","last_name":"Tasciyan","first_name":"Saren","full_name":"Tasciyan, Saren"},{"id":"2A67C376-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2862-8372","last_name":"Aguilera Servin","full_name":"Aguilera Servin, Juan L","first_name":"Juan L"},{"last_name":"De Vries","full_name":"De Vries, Ingrid","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","last_name":"Hauschild","first_name":"Robert","full_name":"Hauschild, Robert"},{"id":"4167FE56-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6625-3348","last_name":"Hons","full_name":"Hons, Miroslav","first_name":"Miroslav"},{"full_name":"Piel, Matthieu","first_name":"Matthieu","last_name":"Piel"},{"first_name":"Andrew","full_name":"Callan-Jones, Andrew","last_name":"Callan-Jones"},{"full_name":"Voituriez, Raphael","first_name":"Raphael","last_name":"Voituriez"},{"last_name":"Sixt","full_name":"Sixt, Michael K","first_name":"Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"}],"oa_version":"None","external_id":{"isi":["000532688300008"]},"date_published":"2020-06-25T00:00:00Z","status":"public","abstract":[{"lang":"eng","text":"Eukaryotic cells migrate by coupling the intracellular force of the actin cytoskeleton to the environment. While force coupling is usually mediated by transmembrane adhesion receptors, especially those of the integrin family, amoeboid cells such as leukocytes can migrate extremely fast despite very low adhesive forces1. Here we show that leukocytes cannot only migrate under low adhesion but can also transmit forces in the complete absence of transmembrane force coupling. When confined within three-dimensional environments, they use the topographical features of the substrate to propel themselves. Here the retrograde flow of the actin cytoskeleton follows the texture of the substrate, creating retrograde shear forces that are sufficient to drive the cell body forwards. Notably, adhesion-dependent and adhesion-independent migration are not mutually exclusive, but rather are variants of the same principle of coupling retrograde actin flow to the environment and thus can potentially operate interchangeably and simultaneously. As adhesion-free migration is independent of the chemical composition of the environment, it renders cells completely autonomous in their locomotive behaviour."}],"ec_funded":1,"intvolume":"       582","citation":{"chicago":"Reversat, Anne, Florian R Gärtner, Jack Merrin, Julian A Stopp, Saren Tasciyan, Juan L Aguilera Servin, Ingrid de Vries, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>. Springer Nature, 2020. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>.","apa":"Reversat, A., Gärtner, F. R., Merrin, J., Stopp, J. A., Tasciyan, S., Aguilera Servin, J. L., … Sixt, M. K. (2020). Cellular locomotion using environmental topography. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/s41586-020-2283-z\">https://doi.org/10.1038/s41586-020-2283-z</a>","ista":"Reversat A, Gärtner FR, Merrin J, Stopp JA, Tasciyan S, Aguilera Servin JL, de Vries I, Hauschild R, Hons M, Piel M, Callan-Jones A, Voituriez R, Sixt MK. 2020. Cellular locomotion using environmental topography. Nature. 582, 582–585.","ieee":"A. Reversat <i>et al.</i>, “Cellular locomotion using environmental topography,” <i>Nature</i>, vol. 582. Springer Nature, pp. 582–585, 2020.","mla":"Reversat, Anne, et al. “Cellular Locomotion Using Environmental Topography.” <i>Nature</i>, vol. 582, Springer Nature, 2020, pp. 582–585, doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>.","short":"A. Reversat, F.R. Gärtner, J. Merrin, J.A. Stopp, S. Tasciyan, J.L. Aguilera Servin, I. de Vries, R. Hauschild, M. Hons, M. Piel, A. Callan-Jones, R. Voituriez, M.K. Sixt, Nature 582 (2020) 582–585.","ama":"Reversat A, Gärtner FR, Merrin J, et al. Cellular locomotion using environmental topography. <i>Nature</i>. 2020;582:582–585. doi:<a href=\"https://doi.org/10.1038/s41586-020-2283-z\">10.1038/s41586-020-2283-z</a>"},"day":"25","related_material":{"link":[{"description":"News on IST Homepage","url":"https://ist.ac.at/en/news/off-road-mode-enables-mobile-cells-to-move-freely/","relation":"press_release"}],"record":[{"status":"public","relation":"dissertation_contains","id":"14697"},{"id":"12401","status":"public","relation":"dissertation_contains"}]},"title":"Cellular locomotion using environmental topography","publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"7885","month":"06","acknowledgement":"We thank A. Leithner and J. Renkawitz for discussion and critical reading of the manuscript; J. Schwarz and M. Mehling for establishing the microfluidic setups; the Bioimaging Facility of IST Austria for excellent support, as well as the Life Science Facility and the Miba Machine Shop of IST Austria; and F. N. Arslan, L. E. Burnett and L. Li for their work during their rotation in the IST PhD programme. This work was supported by the European Research Council (ERC StG 281556 and CoG 724373) to M.S. and grants from the Austrian Science Fund (FWF P29911) and the WWTF to M.S. M.H. was supported by the European Regional Development Fund Project (CZ.02.1.01/0.0/0.0/15_003/0000476). F.G. received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 747687.","department":[{"_id":"NanoFab"},{"_id":"Bio"},{"_id":"MiSi"}]},{"publication_identifier":{"eissn":["2050084X"]},"scopus_import":"1","article_processing_charge":"No","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":9,"file":[{"file_size":10535713,"creator":"dernst","relation":"main_file","checksum":"d33bd4441b9a0195718ce1ba5d2c48a6","date_created":"2020-06-02T10:35:37Z","file_id":"7914","content_type":"application/pdf","date_updated":"2020-07-14T12:48:05Z","access_level":"open_access","file_name":"2020_eLife_Damiano_Guercio.pdf"}],"isi":1,"quality_controlled":"1","year":"2020","date_created":"2020-05-31T22:00:49Z","date_updated":"2023-08-21T06:32:25Z","project":[{"_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular navigation along spatial gradients","grant_number":"724373","call_identifier":"H2020"}],"publication":"eLife","ddc":["570"],"author":[{"last_name":"Damiano-Guercio","first_name":"Julia","full_name":"Damiano-Guercio, Julia"},{"first_name":"Laëtitia","full_name":"Kurzawa, Laëtitia","last_name":"Kurzawa"},{"first_name":"Jan","full_name":"Müller, Jan","last_name":"Müller","id":"AD07FDB4-0F61-11EA-8158-C4CC64CEAA8D"},{"last_name":"Dimchev","first_name":"Georgi A","full_name":"Dimchev, Georgi A","id":"38C393BE-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8370-6161"},{"full_name":"Schaks, Matthias","first_name":"Matthias","last_name":"Schaks"},{"id":"34E27F1C-F248-11E8-B48F-1D18A9856A87","last_name":"Nemethova","first_name":"Maria","full_name":"Nemethova, Maria"},{"last_name":"Pokrant","full_name":"Pokrant, Thomas","first_name":"Thomas"},{"first_name":"Stefan","full_name":"Brühmann, Stefan","last_name":"Brühmann"},{"last_name":"Linkner","full_name":"Linkner, Joern","first_name":"Joern"},{"full_name":"Blanchoin, Laurent","first_name":"Laurent","last_name":"Blanchoin"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt"},{"last_name":"Rottner","first_name":"Klemens","full_name":"Rottner, Klemens"},{"last_name":"Faix","full_name":"Faix, Jan","first_name":"Jan"}],"oa_version":"Published Version","external_id":{"isi":["000537208000001"]},"date_published":"2020-05-11T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:48:05Z","type":"journal_article","doi":"10.7554/eLife.55351","publisher":"eLife Sciences Publications","intvolume":"         9","citation":{"ama":"Damiano-Guercio J, Kurzawa L, Müller J, et al. Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. <i>eLife</i>. 2020;9. doi:<a href=\"https://doi.org/10.7554/eLife.55351\">10.7554/eLife.55351</a>","short":"J. Damiano-Guercio, L. Kurzawa, J. Müller, G.A. Dimchev, M. Schaks, M. Nemethova, T. Pokrant, S. Brühmann, J. Linkner, L. Blanchoin, M.K. Sixt, K. Rottner, J. Faix, ELife 9 (2020).","ieee":"J. Damiano-Guercio <i>et al.</i>, “Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion,” <i>eLife</i>, vol. 9. eLife Sciences Publications, 2020.","mla":"Damiano-Guercio, Julia, et al. “Loss of Ena/VASP Interferes with Lamellipodium Architecture, Motility and Integrin-Dependent Adhesion.” <i>ELife</i>, vol. 9, e55351, eLife Sciences Publications, 2020, doi:<a href=\"https://doi.org/10.7554/eLife.55351\">10.7554/eLife.55351</a>.","ista":"Damiano-Guercio J, Kurzawa L, Müller J, Dimchev GA, Schaks M, Nemethova M, Pokrant T, Brühmann S, Linkner J, Blanchoin L, Sixt MK, Rottner K, Faix J. 2020. Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. eLife. 9, e55351.","apa":"Damiano-Guercio, J., Kurzawa, L., Müller, J., Dimchev, G. A., Schaks, M., Nemethova, M., … Faix, J. (2020). Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion. <i>ELife</i>. eLife Sciences Publications. <a href=\"https://doi.org/10.7554/eLife.55351\">https://doi.org/10.7554/eLife.55351</a>","chicago":"Damiano-Guercio, Julia, Laëtitia Kurzawa, Jan Müller, Georgi A Dimchev, Matthias Schaks, Maria Nemethova, Thomas Pokrant, et al. “Loss of Ena/VASP Interferes with Lamellipodium Architecture, Motility and Integrin-Dependent Adhesion.” <i>ELife</i>. eLife Sciences Publications, 2020. <a href=\"https://doi.org/10.7554/eLife.55351\">https://doi.org/10.7554/eLife.55351</a>."},"day":"11","has_accepted_license":"1","article_number":"e55351","status":"public","abstract":[{"lang":"eng","text":"Cell migration entails networks and bundles of actin filaments termed lamellipodia and microspikes or filopodia, respectively, as well as focal adhesions, all of which recruit Ena/VASP family members hitherto thought to antagonize efficient cell motility. However, we find these proteins to act as positive regulators of migration in different murine cell lines. CRISPR/Cas9-mediated loss of Ena/VASP proteins reduced lamellipodial actin assembly and perturbed lamellipodial architecture, as evidenced by changed network geometry as well as reduction of filament length and number that was accompanied by abnormal Arp2/3 complex and heterodimeric capping protein accumulation. Loss of Ena/VASP function also abolished the formation of microspikes normally embedded in lamellipodia, but not of filopodia capable of emanating without lamellipodia. Ena/VASP-deficiency also impaired integrin-mediated adhesion accompanied by reduced traction forces exerted through these structures. Our data thus uncover novel Ena/VASP functions of these actin polymerases that are fully consistent with their promotion of cell migration."}],"ec_funded":1,"department":[{"_id":"MiSi"}],"oa":1,"title":"Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion","publication_status":"published","article_type":"original","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"7909","month":"05"},{"type":"journal_article","language":[{"iso":"eng"}],"publisher":"AAAS","doi":"10.1126/sciimmunol.abc3979","author":[{"full_name":"Salzer, Elisabeth","first_name":"Elisabeth","last_name":"Salzer"},{"last_name":"Zoghi","first_name":"Samaneh","full_name":"Zoghi, Samaneh"},{"full_name":"Kiss, Máté G.","first_name":"Máté G.","last_name":"Kiss"},{"last_name":"Kage","first_name":"Frieda","full_name":"Kage, Frieda"},{"first_name":"Christina","full_name":"Rashkova, Christina","last_name":"Rashkova"},{"first_name":"Stephanie","full_name":"Stahnke, Stephanie","last_name":"Stahnke"},{"full_name":"Haimel, Matthias","first_name":"Matthias","last_name":"Haimel"},{"last_name":"Platzer","first_name":"René","full_name":"Platzer, René"},{"last_name":"Caldera","full_name":"Caldera, Michael","first_name":"Michael"},{"first_name":"Rico Chandra","full_name":"Ardy, Rico Chandra","last_name":"Ardy"},{"full_name":"Hoeger, Birgit","first_name":"Birgit","last_name":"Hoeger"},{"last_name":"Block","first_name":"Jana","full_name":"Block, Jana"},{"last_name":"Medgyesi","first_name":"David","full_name":"Medgyesi, David"},{"full_name":"Sin, Celine","first_name":"Celine","last_name":"Sin"},{"last_name":"Shahkarami","first_name":"Sepideh","full_name":"Shahkarami, Sepideh"},{"first_name":"Renate","full_name":"Kain, Renate","last_name":"Kain"},{"full_name":"Ziaee, Vahid","first_name":"Vahid","last_name":"Ziaee"},{"last_name":"Hammerl","first_name":"Peter","full_name":"Hammerl, Peter"},{"full_name":"Bock, Christoph","first_name":"Christoph","last_name":"Bock"},{"last_name":"Menche","full_name":"Menche, Jörg","first_name":"Jörg"},{"last_name":"Dupré","full_name":"Dupré, Loïc","first_name":"Loïc"},{"first_name":"Johannes B.","full_name":"Huppa, Johannes B.","last_name":"Huppa"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt"},{"first_name":"Alexis","full_name":"Lomakin, Alexis","last_name":"Lomakin"},{"last_name":"Rottner","full_name":"Rottner, Klemens","first_name":"Klemens"},{"first_name":"Christoph J.","full_name":"Binder, Christoph J.","last_name":"Binder"},{"first_name":"Theresia E.B.","full_name":"Stradal, Theresia E.B.","last_name":"Stradal"},{"last_name":"Rezaei","first_name":"Nima","full_name":"Rezaei, Nima"},{"full_name":"Boztug, Kaan","first_name":"Kaan","last_name":"Boztug"}],"issue":"49","publication":"Science Immunology","date_published":"2020-07-10T00:00:00Z","external_id":{"isi":["000546994600004"],"pmid":["32646852"]},"oa_version":"None","isi":1,"volume":5,"date_updated":"2023-08-22T07:56:04Z","date_created":"2020-07-19T22:00:58Z","year":"2020","quality_controlled":"1","pmid":1,"scopus_import":"1","publication_identifier":{"eissn":["24709468"]},"article_processing_charge":"No","article_type":"original","publication_status":"published","title":"The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity","month":"07","_id":"8132","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","department":[{"_id":"MiSi"}],"abstract":[{"lang":"eng","text":"The WAVE regulatory complex (WRC) is crucial for assembly of the peripheral branched actin network constituting one of the main drivers of eukaryotic cell migration. Here, we uncover an essential role of the hematopoietic-specific WRC component HEM1 for immune cell development. Germline-encoded HEM1 deficiency underlies an inborn error of immunity with systemic autoimmunity, at cellular level marked by WRC destabilization, reduced filamentous actin, and failure to assemble lamellipodia. Hem1−/− mice display systemic autoimmunity, phenocopying the human disease. In the absence of Hem1, B cells become deprived of extracellular stimuli necessary to maintain the strength of B cell receptor signaling at a level permissive for survival of non-autoreactive B cells. This shifts the balance of B cell fate choices toward autoreactive B cells and thus autoimmunity."}],"status":"public","article_number":"eabc3979","intvolume":"         5","citation":{"mla":"Salzer, Elisabeth, et al. “The Cytoskeletal Regulator HEM1 Governs B Cell Development and Prevents Autoimmunity.” <i>Science Immunology</i>, vol. 5, no. 49, eabc3979, AAAS, 2020, doi:<a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">10.1126/sciimmunol.abc3979</a>.","ieee":"E. Salzer <i>et al.</i>, “The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity,” <i>Science Immunology</i>, vol. 5, no. 49. AAAS, 2020.","ista":"Salzer E, Zoghi S, Kiss MG, Kage F, Rashkova C, Stahnke S, Haimel M, Platzer R, Caldera M, Ardy RC, Hoeger B, Block J, Medgyesi D, Sin C, Shahkarami S, Kain R, Ziaee V, Hammerl P, Bock C, Menche J, Dupré L, Huppa JB, Sixt MK, Lomakin A, Rottner K, Binder CJ, Stradal TEB, Rezaei N, Boztug K. 2020. The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity. Science Immunology. 5(49), eabc3979.","apa":"Salzer, E., Zoghi, S., Kiss, M. G., Kage, F., Rashkova, C., Stahnke, S., … Boztug, K. (2020). The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity. <i>Science Immunology</i>. AAAS. <a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">https://doi.org/10.1126/sciimmunol.abc3979</a>","chicago":"Salzer, Elisabeth, Samaneh Zoghi, Máté G. Kiss, Frieda Kage, Christina Rashkova, Stephanie Stahnke, Matthias Haimel, et al. “The Cytoskeletal Regulator HEM1 Governs B Cell Development and Prevents Autoimmunity.” <i>Science Immunology</i>. AAAS, 2020. <a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">https://doi.org/10.1126/sciimmunol.abc3979</a>.","ama":"Salzer E, Zoghi S, Kiss MG, et al. The cytoskeletal regulator HEM1 governs B cell development and prevents autoimmunity. <i>Science Immunology</i>. 2020;5(49). doi:<a href=\"https://doi.org/10.1126/sciimmunol.abc3979\">10.1126/sciimmunol.abc3979</a>","short":"E. Salzer, S. Zoghi, M.G. Kiss, F. Kage, C. Rashkova, S. Stahnke, M. Haimel, R. Platzer, M. Caldera, R.C. Ardy, B. Hoeger, J. Block, D. Medgyesi, C. Sin, S. Shahkarami, R. Kain, V. Ziaee, P. Hammerl, C. Bock, J. Menche, L. Dupré, J.B. Huppa, M.K. Sixt, A. Lomakin, K. Rottner, C.J. Binder, T.E.B. Stradal, N. Rezaei, K. Boztug, Science Immunology 5 (2020)."},"day":"10"},{"language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2020-12-02T09:13:23Z","doi":"10.15252/embj.2019104238","publisher":"Embo Press","issue":"17","publication":"The Embo Journal","author":[{"orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","first_name":"Juan C","full_name":"Montesinos López, Juan C","last_name":"Montesinos López"},{"first_name":"A","full_name":"Abuzeineh, A","last_name":"Abuzeineh"},{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","first_name":"Aglaja","full_name":"Kopf, Aglaja","last_name":"Kopf"},{"orcid":"0000-0002-1009-9652","id":"40F05888-F248-11E8-B48F-1D18A9856A87","first_name":"Alba","full_name":"Juanes Garcia, Alba","last_name":"Juanes Garcia"},{"id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","last_name":"Ötvös","full_name":"Ötvös, Krisztina","first_name":"Krisztina"},{"full_name":"Petrášek, J","first_name":"J","last_name":"Petrášek"},{"first_name":"Michael K","full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","last_name":"Benková","full_name":"Benková, Eva","first_name":"Eva"}],"ddc":["580"],"external_id":{"isi":["000548311800001"],"pmid":["32667089"]},"oa_version":"Published Version","date_published":"2020-09-01T00:00:00Z","isi":1,"file":[{"success":1,"date_updated":"2020-12-02T09:13:23Z","access_level":"open_access","date_created":"2020-12-02T09:13:23Z","file_id":"8827","content_type":"application/pdf","file_name":"2020_EMBO_Montesinos.pdf","creator":"dernst","file_size":3497156,"checksum":"43d2b36598708e6ab05c69074e191d57","relation":"main_file"}],"volume":39,"year":"2020","date_created":"2020-07-21T09:08:38Z","quality_controlled":"1","project":[{"_id":"253E54C8-B435-11E9-9278-68D0E5697425","grant_number":"ALTF710-2016","name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants"},{"call_identifier":"FWF","_id":"2542D156-B435-11E9-9278-68D0E5697425","grant_number":"I 1774-B16","name":"Hormone cross-talk drives nutrient dependent plant development"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"date_updated":"2023-09-05T13:05:47Z","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"pmid":1,"scopus_import":"1","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"article_processing_charge":"Yes (via OA deal)","publication_status":"published","oa":1,"title":"Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage","article_type":"original","month":"09","_id":"8142","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","acknowledgement":"We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring discussions, and Martine De Cock for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship (ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738 to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.","department":[{"_id":"MiSi"},{"_id":"EvBe"}],"status":"public","article_number":"e104238","abstract":[{"lang":"eng","text":"Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells."}],"intvolume":"        39","citation":{"mla":"Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>.","ieee":"J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020.","apa":"Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>","chicago":"Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>.","ista":"Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238.","ama":"Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>","short":"J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020)."},"day":"01","has_accepted_license":"1"},{"publication":"The Journal of Cell Biology","issue":"8","author":[{"full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"first_name":"Anna","full_name":"Huttenlocher, Anna","last_name":"Huttenlocher"}],"ddc":["570"],"external_id":{"isi":["000573631000004"]},"oa_version":"Published Version","date_published":"2020-07-22T00:00:00Z","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2021-02-02T23:30:03Z","doi":"10.1083/jcb.202007029","publisher":"Rockefeller University Press","publication_identifier":{"eissn":["1540-8140"]},"scopus_import":"1","article_processing_charge":"No","tmp":{"short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)"},"file":[{"file_name":"2020_JCB_Sixt.pdf","embargo":"2021-02-01","file_id":"8200","date_created":"2020-08-04T13:11:52Z","content_type":"application/pdf","access_level":"open_access","date_updated":"2021-02-02T23:30:03Z","relation":"main_file","checksum":"30016d778d266b8e17d01094917873b8","file_size":830725,"creator":"dernst"}],"isi":1,"volume":219,"year":"2020","date_created":"2020-08-02T22:00:57Z","date_updated":"2023-10-17T10:04:49Z","department":[{"_id":"MiSi"}],"publication_status":"published","title":"Zena Werb (1945-2020): Cell biology in context","oa":1,"article_type":"letter_note","month":"07","_id":"8190","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"       219","citation":{"short":"M.K. Sixt, A. Huttenlocher, The Journal of Cell Biology 219 (2020).","ama":"Sixt MK, Huttenlocher A. Zena Werb (1945-2020): Cell biology in context. <i>The Journal of Cell Biology</i>. 2020;219(8). doi:<a href=\"https://doi.org/10.1083/jcb.202007029\">10.1083/jcb.202007029</a>","ista":"Sixt MK, Huttenlocher A. 2020. Zena Werb (1945-2020): Cell biology in context. The Journal of Cell Biology. 219(8), e202007029.","apa":"Sixt, M. K., &#38; Huttenlocher, A. (2020). Zena Werb (1945-2020): Cell biology in context. <i>The Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.202007029\">https://doi.org/10.1083/jcb.202007029</a>","chicago":"Sixt, Michael K, and Anna Huttenlocher. “Zena Werb (1945-2020): Cell Biology in Context.” <i>The Journal of Cell Biology</i>. Rockefeller University Press, 2020. <a href=\"https://doi.org/10.1083/jcb.202007029\">https://doi.org/10.1083/jcb.202007029</a>.","mla":"Sixt, Michael K., and Anna Huttenlocher. “Zena Werb (1945-2020): Cell Biology in Context.” <i>The Journal of Cell Biology</i>, vol. 219, no. 8, e202007029, Rockefeller University Press, 2020, doi:<a href=\"https://doi.org/10.1083/jcb.202007029\">10.1083/jcb.202007029</a>.","ieee":"M. K. Sixt and A. Huttenlocher, “Zena Werb (1945-2020): Cell biology in context,” <i>The Journal of Cell Biology</i>, vol. 219, no. 8. Rockefeller University Press, 2020."},"day":"22","has_accepted_license":"1","status":"public","article_number":"e202007029"},{"publication_identifier":{"issn":["2663-337X"]},"article_processing_charge":"No","file":[{"relation":"source_file","checksum":"53a739752a500f84d0f8ec953cbbd0b6","file_size":214172667,"creator":"fassen","file_name":"PhDthesis_FrankAssen_revised2.docx","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","embargo_to":"open_access","file_id":"6990","date_created":"2019-11-06T12:30:02Z","access_level":"closed","date_updated":"2020-11-07T23:30:03Z"},{"file_size":83637532,"creator":"fassen","relation":"main_file","checksum":"8c156b65d9347bb599623a4b09f15d15","content_type":"application/pdf","file_id":"6991","date_created":"2019-11-06T12:30:57Z","access_level":"open_access","date_updated":"2020-11-07T23:30:03Z","file_name":"PhDthesis_FrankAssen_revised2.pdf","embargo":"2020-11-06"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"},{"_id":"EM-Fac"}],"date_updated":"2023-09-13T08:50:57Z","year":"2019","date_created":"2019-10-14T16:54:52Z","page":"142","author":[{"full_name":"Assen, Frank P","first_name":"Frank P","last_name":"Assen","id":"3A8E7F24-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-3470-6119"}],"ddc":["570"],"date_published":"2019-10-09T00:00:00Z","oa_version":"Published Version","type":"dissertation","file_date_updated":"2020-11-07T23:30:03Z","language":[{"iso":"eng"}],"publisher":"Institute of Science and Technology Austria","doi":"10.15479/AT:ISTA:6947","citation":{"mla":"Assen, Frank P. <i>Lymph Node Mechanics: Deciphering the Interplay between Stroma Contractility, Morphology and Lymphocyte Trafficking</i>. Institute of Science and Technology Austria, 2019, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:6947\">10.15479/AT:ISTA:6947</a>.","ieee":"F. P. Assen, “Lymph node mechanics: Deciphering the interplay between stroma contractility, morphology and lymphocyte trafficking,” Institute of Science and Technology Austria, 2019.","ista":"Assen FP. 2019. Lymph node mechanics: Deciphering the interplay between stroma contractility, morphology and lymphocyte trafficking. Institute of Science and Technology Austria.","chicago":"Assen, Frank P. “Lymph Node Mechanics: Deciphering the Interplay between Stroma Contractility, Morphology and Lymphocyte Trafficking.” Institute of Science and Technology Austria, 2019. <a href=\"https://doi.org/10.15479/AT:ISTA:6947\">https://doi.org/10.15479/AT:ISTA:6947</a>.","apa":"Assen, F. P. (2019). <i>Lymph node mechanics: Deciphering the interplay between stroma contractility, morphology and lymphocyte trafficking</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:6947\">https://doi.org/10.15479/AT:ISTA:6947</a>","ama":"Assen FP. Lymph node mechanics: Deciphering the interplay between stroma contractility, morphology and lymphocyte trafficking. 2019. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:6947\">10.15479/AT:ISTA:6947</a>","short":"F.P. Assen, Lymph Node Mechanics: Deciphering the Interplay between Stroma Contractility, Morphology and Lymphocyte Trafficking, Institute of Science and Technology Austria, 2019."},"supervisor":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"}],"has_accepted_license":"1","day":"9","abstract":[{"text":"Lymph nodes  are es s ential organs  of the immune  s ys tem where adaptive immune responses originate, and consist of various leukocyte populations and a stromal backbone. Fibroblastic reticular  cells (FRCs) are  the  main  stromal  cells and  form  a sponge-like extracellular matrix network,   called  conduits ,  which  they   thems elves   enwrap   and  contract.  Lymph,  containing  s oluble  antigens ,  arrive in  lymph  nodes  via afferent lymphatic  vessels that  connect  to  the  s ubcaps ular  s inus   and  conduit  network.  According  to  the  current  paradigm,  the  conduit  network   dis tributes   afferent  lymph  through   lymph  nodes   and  thus   provides   acces s   for  immune  cells to lymph-borne  antigens. An  elas tic  caps ule  s urrounds   the  organ  and  confines   the immune  cells and  FRC  network.   Lymph   nodes   are  completely  packed  with  lymphocytes   and  lymphocyte  numbers  directly  dictates  the size  of  the  organ.  Although  lymphocytes   cons tantly  enter  and  leave  the  lymph  node,  its   s ize  remains   remarkedly   s table  under  homeostatic conditions. It is only partly known  how the cellularity and s ize of the lymph node is regulated and  how  the  lymph  node  is able to swell in inflammation.  The role of the FRC network   in  lymph  node   s welling  and  trans fer  of  fluids   are  inves tigated in  this   thes is.  Furthermore,   we  s tudied  what  trafficking  routes   are  us ed  by  cancer  cells   in  lymph  nodes   to  form  distal metastases.We examined the role of a mechanical feedback in regulation of lymph  node swelling. Using parallel plate compression  and UV-las er  cutting  experiments   we  dis s ected  the  mechanical  force dynamics  of the whole lymph  node, and individually for FRCs  and the  caps ule. Physical forces   generated  by  packed  lymphocytes   directly  affect  the  tens ion  on  the  FRC  network  and  capsule,  which  increases  its  resistance  to   swelling.  This  implies  a  feedback  mechanism  between   tis s ue   pres s ure   and   ability   of   lymphocytes    to   enter   the   organ.   Following   inflammation,  the  lymph  node  swells ∼10 fold in two weeks . Yet, what  is  the role  for tens ion on  the  FRC  network   and  caps ule,  and  how  are  lymphocytes   able  to  enter  in  conditions  that resist swelling remain open ques tions . We s how that tens ion on the FRC network is  important to  limit  the  swelling  rate  of  the  organ  so  that  the  FRC  network  can  grow  in  a  coordinated  fashion. This is illustrated by interfering with FRC contractility, which leads to faster swelling rates  and a dis organized FRC network  in the inflamed lymph  node. Growth  of the FRC network  in  turn  is   expected  to  releas e  tens ion  on  thes e  s tructures   and  lowers   the  res is tance  to  swelling, thereby allowing more lymphocytes to enter the organ and drive more swelling. Halt of  swelling coincides   with  a  thickening  of  the  caps ule,  which  forms   a  thick  res is tant  band  around  the organ and lowers  tens ion on the FRC network  to form a new force equilibrium.The  FRC  and  conduit   network   are  further   believed  to  be  a  privileged  s ite  of  s oluble  information  within  the  lymph  node,  although  many  details   remain  uns olved.  We  s how  by  3D  ultra-recons truction   that  FRCs   and  antigen  pres enting  cells   cover  the  s urface  of  conduit  s ys tem for more  than 99% and we dis cus s  the implications  for s oluble information  exchangeat the conduit level.Finally, there  is an ongoing debate in the cancer field whether and how cancer cells  in lymph nodes   s eed  dis tal  metas tas es .  We  s how  that  cancer  cells   infus ed  into  the  lymph  node  can  utilize trafficking routes of immune  cells and  rapidly  migrate  to  blood  vessels. Once  in  the  blood circulation,  these cells are able to form  metastases in distal tissues.","lang":"eng"}],"status":"public","degree_awarded":"PhD","alternative_title":["ISTA Thesis"],"department":[{"_id":"MiSi"}],"publication_status":"published","related_material":{"record":[{"status":"public","relation":"part_of_dissertation","id":"664"},{"id":"402","status":"public","relation":"part_of_dissertation"}]},"title":"Lymph node mechanics: Deciphering the interplay between stroma contractility, morphology and lymphocyte trafficking","oa":1,"_id":"6947","month":"10","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1"},{"status":"public","day":"21","citation":{"short":"A. Kopf, M.K. Sixt, Current Biology 29 (2019) R1091–R1093.","ama":"Kopf A, Sixt MK. Gut homeostasis: Active migration of intestinal epithelial cells in tissue renewal. <i>Current Biology</i>. 2019;29(20):R1091-R1093. doi:<a href=\"https://doi.org/10.1016/j.cub.2019.08.068\">10.1016/j.cub.2019.08.068</a>","chicago":"Kopf, Aglaja, and Michael K Sixt. “Gut Homeostasis: Active Migration of Intestinal Epithelial Cells in Tissue Renewal.” <i>Current Biology</i>. Cell Press, 2019. <a href=\"https://doi.org/10.1016/j.cub.2019.08.068\">https://doi.org/10.1016/j.cub.2019.08.068</a>.","apa":"Kopf, A., &#38; Sixt, M. K. (2019). Gut homeostasis: Active migration of intestinal epithelial cells in tissue renewal. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2019.08.068\">https://doi.org/10.1016/j.cub.2019.08.068</a>","ista":"Kopf A, Sixt MK. 2019. Gut homeostasis: Active migration of intestinal epithelial cells in tissue renewal. Current Biology. 29(20), R1091–R1093.","mla":"Kopf, Aglaja, and Michael K. Sixt. “Gut Homeostasis: Active Migration of Intestinal Epithelial Cells in Tissue Renewal.” <i>Current Biology</i>, vol. 29, no. 20, Cell Press, 2019, pp. R1091–93, doi:<a href=\"https://doi.org/10.1016/j.cub.2019.08.068\">10.1016/j.cub.2019.08.068</a>.","ieee":"A. Kopf and M. K. Sixt, “Gut homeostasis: Active migration of intestinal epithelial cells in tissue renewal,” <i>Current Biology</i>, vol. 29, no. 20. Cell Press, pp. R1091–R1093, 2019."},"intvolume":"        29","_id":"6979","month":"10","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","article_type":"original","publication_status":"published","title":"Gut homeostasis: Active migration of intestinal epithelial cells in tissue renewal","department":[{"_id":"MiSi"}],"date_updated":"2023-09-05T12:43:43Z","year":"2019","date_created":"2019-11-04T15:18:29Z","page":"R1091-R1093","quality_controlled":"1","isi":1,"volume":29,"article_processing_charge":"No","pmid":1,"scopus_import":"1","publication_identifier":{"eissn":["1879-0445"],"issn":["0960-9822"]},"publisher":"Cell Press","doi":"10.1016/j.cub.2019.08.068","type":"journal_article","language":[{"iso":"eng"}],"date_published":"2019-10-21T00:00:00Z","external_id":{"pmid":["31639357"],"isi":["000491286200016"]},"oa_version":"None","author":[{"id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","first_name":"Aglaja","last_name":"Kopf"},{"orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K"}],"issue":"20","publication":"Current Biology"}]