[{"file":[{"relation":"main_file","access_level":"open_access","file_id":"4915","creator":"system","date_created":"2018-12-12T10:11:58Z","checksum":"bae12e86be2adb28253f890b8bba8315","file_size":4530215,"date_updated":"2020-07-14T12:45:02Z","file_name":"IST-2016-476-v1+1_ncomms8526.pdf","content_type":"application/pdf"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"type":"journal_article","date_published":"2015-06-25T00:00:00Z","publist_id":"5596","oa":1,"language":[{"iso":"eng"}],"has_accepted_license":"1","publication":"Nature Communications","oa_version":"Published Version","article_number":"7526","month":"06","volume":6,"acknowledgement":"M.C. and M.L.H. were supported by fellowships from the Fondation pour la Recherche Médicale and the Association pour la Recherche contre le Cancer, respectively. This work was funded by grants from the City of Paris and the European Research Council to A.-M.L.-D. (Strapacemi 243103), the Association Nationale pour la Recherche (ANR-09-PIRI-0027-PCVI) and the InnaBiosanté foundation (Micemico) to A.-M.L.-D., M.P. and R.V., and the DCBIOL Labex from the French Government (ANR-10-IDEX-0001-02-PSL* and ANR-11-LABX-0043). The super-resolution SIM microscope was funded through an ERC Advanced Investigator Grant (250367) to Edith Heard (CNRS UMR3215/Inserm U934, Institut Curie).","ddc":["570"],"citation":{"mla":"Chabaud, Mélanie, et al. “Cell Migration and Antigen Capture Are Antagonistic Processes Coupled by Myosin II in Dendritic Cells.” <i>Nature Communications</i>, vol. 6, 7526, Nature Publishing Group, 2015, doi:<a href=\"https://doi.org/10.1038/ncomms8526\">10.1038/ncomms8526</a>.","short":"M. Chabaud, M. Heuzé, M. Bretou, P. Vargas, P. Maiuri, P. Solanes, M. Maurin, E. Terriac, M. Le Berre, D. Lankar, T. Piolot, R. Adelstein, Y. Zhang, M.K. Sixt, J. Jacobelli, O. Bénichou, R. Voituriez, M. Piel, A. Lennon Duménil, Nature Communications 6 (2015).","ista":"Chabaud M, Heuzé M, Bretou M, Vargas P, Maiuri P, Solanes P, Maurin M, Terriac E, Le Berre M, Lankar D, Piolot T, Adelstein R, Zhang Y, Sixt MK, Jacobelli J, Bénichou O, Voituriez R, Piel M, Lennon Duménil A. 2015. Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. Nature Communications. 6, 7526.","apa":"Chabaud, M., Heuzé, M., Bretou, M., Vargas, P., Maiuri, P., Solanes, P., … Lennon Duménil, A. (2015). Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. <i>Nature Communications</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncomms8526\">https://doi.org/10.1038/ncomms8526</a>","ama":"Chabaud M, Heuzé M, Bretou M, et al. Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells. <i>Nature Communications</i>. 2015;6. doi:<a href=\"https://doi.org/10.1038/ncomms8526\">10.1038/ncomms8526</a>","ieee":"M. Chabaud <i>et al.</i>, “Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells,” <i>Nature Communications</i>, vol. 6. Nature Publishing Group, 2015.","chicago":"Chabaud, Mélanie, Mélina Heuzé, Marine Bretou, Pablo Vargas, Paolo Maiuri, Paola Solanes, Mathieu Maurin, et al. “Cell Migration and Antigen Capture Are Antagonistic Processes Coupled by Myosin II in Dendritic Cells.” <i>Nature Communications</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/ncomms8526\">https://doi.org/10.1038/ncomms8526</a>."},"year":"2015","date_updated":"2021-01-12T06:51:42Z","day":"25","doi":"10.1038/ncomms8526","abstract":[{"text":"The immune response relies on the migration of leukocytes and on their ability to stop in precise anatomical locations to fulfil their task. How leukocyte migration and function are coordinated is unknown. Here we show that in immature dendritic cells, which patrol their environment by engulfing extracellular material, cell migration and antigen capture are antagonistic. This antagonism results from transient enrichment of myosin IIA at the cell front, which disrupts the back-to-front gradient of the motor protein, slowing down locomotion but promoting antigen capture. We further highlight that myosin IIA enrichment at the cell front requires the MHC class II-associated invariant chain (Ii). Thus, by controlling myosin IIA localization, Ii imposes on dendritic cells an intermittent antigen capture behaviour that might facilitate environment patrolling. We propose that the requirement for myosin II in both cell migration and specific cell functions may provide a general mechanism for their coordination in time and space.","lang":"eng"}],"quality_controlled":"1","file_date_updated":"2020-07-14T12:45:02Z","publisher":"Nature Publishing Group","scopus_import":1,"_id":"1575","author":[{"first_name":"Mélanie","last_name":"Chabaud","full_name":"Chabaud, Mélanie"},{"full_name":"Heuzé, Mélina","first_name":"Mélina","last_name":"Heuzé"},{"last_name":"Bretou","first_name":"Marine","full_name":"Bretou, Marine"},{"last_name":"Vargas","first_name":"Pablo","full_name":"Vargas, Pablo"},{"last_name":"Maiuri","first_name":"Paolo","full_name":"Maiuri, Paolo"},{"first_name":"Paola","last_name":"Solanes","full_name":"Solanes, Paola"},{"last_name":"Maurin","first_name":"Mathieu","full_name":"Maurin, Mathieu"},{"full_name":"Terriac, Emmanuel","last_name":"Terriac","first_name":"Emmanuel"},{"last_name":"Le Berre","first_name":"Maël","full_name":"Le Berre, Maël"},{"first_name":"Danielle","last_name":"Lankar","full_name":"Lankar, Danielle"},{"last_name":"Piolot","first_name":"Tristan","full_name":"Piolot, Tristan"},{"first_name":"Robert","last_name":"Adelstein","full_name":"Adelstein, Robert"},{"first_name":"Yingfan","last_name":"Zhang","full_name":"Zhang, Yingfan"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"},{"full_name":"Jacobelli, Jordan","last_name":"Jacobelli","first_name":"Jordan"},{"full_name":"Bénichou, Olivier","first_name":"Olivier","last_name":"Bénichou"},{"full_name":"Voituriez, Raphaël","last_name":"Voituriez","first_name":"Raphaël"},{"full_name":"Piel, Matthieu","last_name":"Piel","first_name":"Matthieu"},{"last_name":"Lennon Duménil","first_name":"Ana","full_name":"Lennon Duménil, Ana"}],"date_created":"2018-12-11T11:52:48Z","department":[{"_id":"MiSi"}],"publication_status":"published","intvolume":"         6","title":"Cell migration and antigen capture are antagonistic processes coupled by myosin II in dendritic cells","pubrep_id":"476"},{"abstract":[{"text":"During inflammation, lymph nodes swell with an influx of immune cells. New findings identify a signalling pathway that induces relaxation in the contractile cells that give structure to these organs.","lang":"eng"}],"publist_id":"5219","doi":"10.1038/514441a","day":"23","date_published":"2014-10-23T00:00:00Z","type":"journal_article","date_updated":"2021-01-12T06:53:47Z","year":"2014","citation":{"mla":"Sixt, Michael K., and Kari Vaahtomeri. “Physiology: Relax and Come In.” <i>Nature</i>, vol. 514, no. 7523, Springer Nature, 2014, pp. 441–42, doi:<a href=\"https://doi.org/10.1038/514441a\">10.1038/514441a</a>.","short":"M.K. Sixt, K. Vaahtomeri, Nature 514 (2014) 441–442.","ista":"Sixt MK, Vaahtomeri K. 2014. Physiology: Relax and come in. Nature. 514(7523), 441–442.","ama":"Sixt MK, Vaahtomeri K. Physiology: Relax and come in. <i>Nature</i>. 2014;514(7523):441-442. doi:<a href=\"https://doi.org/10.1038/514441a\">10.1038/514441a</a>","apa":"Sixt, M. K., &#38; Vaahtomeri, K. (2014). Physiology: Relax and come in. <i>Nature</i>. Springer Nature. <a href=\"https://doi.org/10.1038/514441a\">https://doi.org/10.1038/514441a</a>","ieee":"M. K. Sixt and K. Vaahtomeri, “Physiology: Relax and come in,” <i>Nature</i>, vol. 514, no. 7523. Springer Nature, pp. 441–442, 2014.","chicago":"Sixt, Michael K, and Kari Vaahtomeri. “Physiology: Relax and Come In.” <i>Nature</i>. Springer Nature, 2014. <a href=\"https://doi.org/10.1038/514441a\">https://doi.org/10.1038/514441a</a>."},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","volume":514,"month":"10","title":"Physiology: Relax and come in","intvolume":"       514","oa_version":"None","publication_status":"published","date_created":"2018-12-11T11:54:30Z","department":[{"_id":"MiSi"}],"author":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"},{"id":"368EE576-F248-11E8-B48F-1D18A9856A87","full_name":"Vaahtomeri, Kari","orcid":"0000-0001-7829-3518","last_name":"Vaahtomeri","first_name":"Kari"}],"issue":"7523","_id":"1877","publication":"Nature","scopus_import":1,"article_type":"letter_note","publisher":"Springer Nature","language":[{"iso":"eng"}],"page":"441 - 442","quality_controlled":"1"},{"type":"journal_article","date_published":"2014-02-01T00:00:00Z","citation":{"chicago":"Konradi, Sabine, Nighat Yasmin, Denise Haslwanter, Michele Weber, Bernd Gesslbauer, Michael K Sixt, and Herbert Strobl. “Langerhans Cell Maturation Is Accompanied by Induction of N-Cadherin and the Transcriptional Regulators of Epithelial-Mesenchymal Transition ZEB1/2.” <i>European Journal of Immunology</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/eji.201343681\">https://doi.org/10.1002/eji.201343681</a>.","ieee":"S. Konradi <i>et al.</i>, “Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2,” <i>European Journal of Immunology</i>, vol. 44, no. 2. Wiley-Blackwell, pp. 553–560, 2014.","apa":"Konradi, S., Yasmin, N., Haslwanter, D., Weber, M., Gesslbauer, B., Sixt, M. K., &#38; Strobl, H. (2014). Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2. <i>European Journal of Immunology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/eji.201343681\">https://doi.org/10.1002/eji.201343681</a>","ama":"Konradi S, Yasmin N, Haslwanter D, et al. Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2. <i>European Journal of Immunology</i>. 2014;44(2):553-560. doi:<a href=\"https://doi.org/10.1002/eji.201343681\">10.1002/eji.201343681</a>","ista":"Konradi S, Yasmin N, Haslwanter D, Weber M, Gesslbauer B, Sixt MK, Strobl H. 2014. Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2. European Journal of Immunology. 44(2), 553–560.","mla":"Konradi, Sabine, et al. “Langerhans Cell Maturation Is Accompanied by Induction of N-Cadherin and the Transcriptional Regulators of Epithelial-Mesenchymal Transition ZEB1/2.” <i>European Journal of Immunology</i>, vol. 44, no. 2, Wiley-Blackwell, 2014, pp. 553–60, doi:<a href=\"https://doi.org/10.1002/eji.201343681\">10.1002/eji.201343681</a>.","short":"S. Konradi, N. Yasmin, D. Haslwanter, M. Weber, B. Gesslbauer, M.K. Sixt, H. Strobl, European Journal of Immunology 44 (2014) 553–560."},"year":"2014","date_updated":"2021-01-12T06:54:01Z","publist_id":"5185","abstract":[{"text":"angerhans cells (LCs) are a unique subset of dendritic cells (DCs) that express epithelial adhesion molecules, allowing them to form contacts with epithelial cells and reside in epidermal/epithelial tissues. The dynamic regulation of epithelial adhesion plays a decisive role in the life cycle of LCs. It controls whether LCs remain immature and sessile within the epidermis or mature and egress to initiate immune responses. So far, the molecular machinery regulating epithelial adhesion molecules during LC maturation remains elusive. Here, we generated pure populations of immature human LCs in vitro to systematically probe for gene-expression changes during LC maturation. LCs down-regulate a set of epithelial genes including E-cadherin, while they upregulate the mesenchymal marker N-cadherin known to facilitate cell migration. In addition, N-cadherin is constitutively expressed by monocyte-derived DCs known to exhibit characteristics of both inflammatory-type and interstitial/dermal DCs. Moreover, the transcription factors ZEB1 and ZEB2 (ZEB is zinc-finger E-box-binding homeobox) are upregulated in migratory LCs. ZEB1 and ZEB2 have been shown to induce epithelial-to-mesenchymal transition (EMT) and invasive behavior in cancer cells undergoing metastasis. Our results provide the first hint that the molecular EMT machinery might facilitate LC mobilization. Moreover, our study suggests that N-cadherin plays a role during DC migration.","lang":"eng"}],"day":"01","doi":"10.1002/eji.201343681","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","acknowledgement":"FWF. Grant Number: P22058-B20","volume":44,"issue":"2","author":[{"last_name":"Konradi","first_name":"Sabine","full_name":"Konradi, Sabine"},{"first_name":"Nighat","last_name":"Yasmin","full_name":"Yasmin, Nighat"},{"last_name":"Haslwanter","first_name":"Denise","full_name":"Haslwanter, Denise"},{"full_name":"Weber, Michele","first_name":"Michele","last_name":"Weber","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Bernd","last_name":"Gesslbauer","full_name":"Gesslbauer, Bernd"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K"},{"full_name":"Strobl, Herbert","first_name":"Herbert","last_name":"Strobl"}],"scopus_import":1,"publication":"European Journal of Immunology","_id":"1910","intvolume":"        44","month":"02","title":"Langerhans cell maturation is accompanied by induction of N-cadherin and the transcriptional regulators of epithelial-mesenchymal transition ZEB1/2","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:54:40Z","oa_version":"None","publication_status":"published","language":[{"iso":"eng"}],"page":"553 - 560","publisher":"Wiley-Blackwell"},{"file_date_updated":"2020-07-14T12:45:21Z","publisher":"IOP Publishing","article_type":"original","_id":"1925","scopus_import":1,"author":[{"first_name":"Constanze","last_name":"Lamprecht","full_name":"Lamprecht, Constanze"},{"full_name":"Plochberger, Birgit","last_name":"Plochberger","first_name":"Birgit"},{"first_name":"Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","full_name":"Wieser, Stefan","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Christian","last_name":"Rankl","full_name":"Rankl, Christian"},{"last_name":"Heister","first_name":"Elena","full_name":"Heister, Elena"},{"full_name":"Unterauer, Barbara","first_name":"Barbara","last_name":"Unterauer"},{"full_name":"Brameshuber, Mario","first_name":"Mario","last_name":"Brameshuber"},{"last_name":"Danzberger","first_name":"Jürgen","full_name":"Danzberger, Jürgen"},{"first_name":"Petar","last_name":"Lukanov","full_name":"Lukanov, Petar"},{"full_name":"Flahaut, Emmanuel","first_name":"Emmanuel","last_name":"Flahaut"},{"full_name":"Schütz, Gerhard","first_name":"Gerhard","last_name":"Schütz"},{"full_name":"Hinterdorfer, Peter","last_name":"Hinterdorfer","first_name":"Peter"},{"full_name":"Ebner, Andreas","last_name":"Ebner","first_name":"Andreas"}],"issue":"12","publication_status":"published","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"date_created":"2018-12-11T11:54:45Z","article_processing_charge":"No","title":"A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes","intvolume":"        25","acknowledgement":"This work was supported by EC grant Marie Curie RTN-CT-2006-035616, CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net 823980, 'IntelliTip'.\r\n","volume":25,"ddc":["570"],"date_updated":"2021-01-12T06:54:07Z","year":"2014","citation":{"ista":"Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P, Ebner A. 2014. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.","short":"C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz, P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).","mla":"Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>, vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>.","chicago":"Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser, Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>. IOP Publishing, 2014. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>.","ieee":"C. Lamprecht <i>et al.</i>, “A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes,” <i>Nanotechnology</i>, vol. 25, no. 12. IOP Publishing, 2014.","apa":"Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister, E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>","ama":"Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. 2014;25(12). doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>"},"doi":"10.1088/0957-4484/25/12/125704","day":"28","abstract":[{"text":"In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity.","lang":"eng"}],"language":[{"iso":"eng"}],"publication":"Nanotechnology","has_accepted_license":"1","oa_version":"Submitted Version","month":"03","article_number":"125704","file":[{"file_id":"7856","creator":"dernst","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:45:21Z","content_type":"application/pdf","file_name":"2014_Nanotechnology_Lamprecht.pdf","date_created":"2020-05-15T09:21:19Z","checksum":"df4e03d225a19179e7790f6d87a12332","file_size":3804152}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2014-03-28T00:00:00Z","type":"journal_article","oa":1,"publist_id":"5169"},{"oa":1,"publist_id":"4848","date_published":"2014-10-01T00:00:00Z","type":"journal_article","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4177954/","open_access":"1"}],"status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","oa_version":"Submitted Version","month":"10","publication":"Current Opinion in Cell Biology","language":[{"iso":"eng"}],"doi":"10.1016/j.ceb.2014.05.010","day":"01","abstract":[{"text":"Directional guidance of migrating cells is relatively well explored in the reductionist setting of cell culture experiments. Here spatial gradients of chemical cues as well as gradients of mechanical substrate characteristics prove sufficient to attract single cells as well as their collectives. How such gradients present and act in the context of an organism is far less clear. Here we review recent advances in understanding how guidance cues emerge and operate in the physiological context.","lang":"eng"}],"date_updated":"2021-01-12T06:55:40Z","citation":{"chicago":"Majumdar, Ritankar, Michael K Sixt, and Carole Parent. “New Paradigms in the Establishment and Maintenance of Gradients during Directed Cell Migration.” <i>Current Opinion in Cell Biology</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.ceb.2014.05.010\">https://doi.org/10.1016/j.ceb.2014.05.010</a>.","ieee":"R. Majumdar, M. K. Sixt, and C. Parent, “New paradigms in the establishment and maintenance of gradients during directed cell migration,” <i>Current Opinion in Cell Biology</i>, vol. 30, no. 1. Elsevier, pp. 33–40, 2014.","ama":"Majumdar R, Sixt MK, Parent C. New paradigms in the establishment and maintenance of gradients during directed cell migration. <i>Current Opinion in Cell Biology</i>. 2014;30(1):33-40. doi:<a href=\"https://doi.org/10.1016/j.ceb.2014.05.010\">10.1016/j.ceb.2014.05.010</a>","apa":"Majumdar, R., Sixt, M. K., &#38; Parent, C. (2014). New paradigms in the establishment and maintenance of gradients during directed cell migration. <i>Current Opinion in Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ceb.2014.05.010\">https://doi.org/10.1016/j.ceb.2014.05.010</a>","ista":"Majumdar R, Sixt MK, Parent C. 2014. New paradigms in the establishment and maintenance of gradients during directed cell migration. Current Opinion in Cell Biology. 30(1), 33–40.","mla":"Majumdar, Ritankar, et al. “New Paradigms in the Establishment and Maintenance of Gradients during Directed Cell Migration.” <i>Current Opinion in Cell Biology</i>, vol. 30, no. 1, Elsevier, 2014, pp. 33–40, doi:<a href=\"https://doi.org/10.1016/j.ceb.2014.05.010\">10.1016/j.ceb.2014.05.010</a>.","short":"R. Majumdar, M.K. Sixt, C. Parent, Current Opinion in Cell Biology 30 (2014) 33–40."},"year":"2014","external_id":{"pmid":["24959970"]},"volume":30,"acknowledgement":"This effort was supported by the Intramural Research Program of the Center for Cancer Research, NCI, National Institutes of Health and the European Research Council (ERC).","publication_status":"published","date_created":"2018-12-11T11:56:03Z","department":[{"_id":"MiSi"}],"title":"New paradigms in the establishment and maintenance of gradients during directed cell migration","intvolume":"        30","_id":"2158","pmid":1,"scopus_import":1,"author":[{"first_name":"Ritankar","last_name":"Majumdar","full_name":"Majumdar, Ritankar"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"},{"last_name":"Parent","first_name":"Carole","full_name":"Parent, Carole"}],"issue":"1","publisher":"Elsevier","page":"33 - 40","quality_controlled":"1"},{"title":"Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects","pubrep_id":"433","intvolume":"         9","publication_status":"published","date_created":"2018-12-11T11:56:22Z","department":[{"_id":"MiSi"}],"author":[{"full_name":"Stoler Barak, Liat","first_name":"Liat","last_name":"Stoler Barak"},{"full_name":"Moussion, Christine","last_name":"Moussion","first_name":"Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Elias","last_name":"Shezen","full_name":"Shezen, Elias"},{"full_name":"Hatzav, Miki","last_name":"Hatzav","first_name":"Miki"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Ronen","last_name":"Alon","full_name":"Alon, Ronen"}],"issue":"1","_id":"2214","scopus_import":1,"publisher":"Public Library of Science","file_date_updated":"2020-07-14T12:45:33Z","ec_funded":1,"quality_controlled":"1","abstract":[{"lang":"eng","text":"A hallmark of immune cell trafficking is directional guidance via gradients of soluble or surface bound chemokines. Vascular endothelial cells produce, transport and deposit either their own chemokines or chemokines produced by the underlying stroma. Endothelial heparan sulfate (HS) was suggested to be a critical scaffold for these chemokine pools, but it is unclear how steep chemokine gradients are sustained between the lumenal and ablumenal aspects of blood vessels. Addressing this question by semi-quantitative immunostaining of HS moieties around blood vessels with a pan anti-HS IgM mAb, we found a striking HS enrichment in the basal lamina of resting and inflamed post capillary skin venules, as well as in high endothelial venules (HEVs) of lymph nodes. Staining of skin vessels with a glycocalyx probe further suggested that their lumenal glycocalyx contains much lower HS density than their basolateral extracellular matrix (ECM). This polarized HS pattern was observed also in isolated resting and inflamed microvascular dermal cells. Notably, progressive skin inflammation resulted in massive ECM deposition and in further HS enrichment around skin post capillary venules and their associated pericytes. Inflammation-dependent HS enrichment was not compromised in mice deficient in the main HS degrading enzyme, heparanase. Our results suggest that the blood vasculature patterns steep gradients of HS scaffolds between their lumenal and basolateral endothelial aspects, and that inflammatory processes can further enrich the HS content nearby inflamed vessels. We propose that chemokine gradients between the lumenal and ablumenal sides of vessels could be favored by these sharp HS scaffold gradients."}],"doi":"10.1371/journal.pone.0085699","day":"22","date_updated":"2021-01-12T06:56:03Z","citation":{"apa":"Stoler Barak, L., Moussion, C., Shezen, E., Hatzav, M., Sixt, M. K., &#38; Alon, R. (2014). Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects. <i>PLoS One</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pone.0085699\">https://doi.org/10.1371/journal.pone.0085699</a>","ama":"Stoler Barak L, Moussion C, Shezen E, Hatzav M, Sixt MK, Alon R. Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects. <i>PLoS One</i>. 2014;9(1). doi:<a href=\"https://doi.org/10.1371/journal.pone.0085699\">10.1371/journal.pone.0085699</a>","chicago":"Stoler Barak, Liat, Christine Moussion, Elias Shezen, Miki Hatzav, Michael K Sixt, and Ronen Alon. “Blood Vessels Pattern Heparan Sulfate Gradients between Their Apical and Basolateral Aspects.” <i>PLoS One</i>. Public Library of Science, 2014. <a href=\"https://doi.org/10.1371/journal.pone.0085699\">https://doi.org/10.1371/journal.pone.0085699</a>.","ieee":"L. Stoler Barak, C. Moussion, E. Shezen, M. Hatzav, M. K. Sixt, and R. Alon, “Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects,” <i>PLoS One</i>, vol. 9, no. 1. Public Library of Science, 2014.","short":"L. Stoler Barak, C. Moussion, E. Shezen, M. Hatzav, M.K. Sixt, R. Alon, PLoS One 9 (2014).","mla":"Stoler Barak, Liat, et al. “Blood Vessels Pattern Heparan Sulfate Gradients between Their Apical and Basolateral Aspects.” <i>PLoS One</i>, vol. 9, no. 1, e85699, Public Library of Science, 2014, doi:<a href=\"https://doi.org/10.1371/journal.pone.0085699\">10.1371/journal.pone.0085699</a>.","ista":"Stoler Barak L, Moussion C, Shezen E, Hatzav M, Sixt MK, Alon R. 2014. Blood vessels pattern heparan sulfate gradients between their apical and basolateral aspects. PLoS One. 9(1), e85699."},"year":"2014","ddc":["570"],"volume":9,"acknowledgement":"Michael Sixt's research is supported by the European Research Council (ERC Starting grant).","month":"01","article_number":"e85699","oa_version":"Published Version","project":[{"call_identifier":"FP7","_id":"25A76F58-B435-11E9-9278-68D0E5697425","grant_number":"289720","name":"Stromal Cell-immune Cell Interactions in Health and Disease"}],"publication":"PLoS One","has_accepted_license":"1","language":[{"iso":"eng"}],"oa":1,"publist_id":"4756","date_published":"2014-01-22T00:00:00Z","type":"journal_article","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","file":[{"date_created":"2018-12-12T10:07:48Z","checksum":"84a8033bda2e07e39405f5acc85f4eca","file_size":12634775,"date_updated":"2020-07-14T12:45:33Z","content_type":"application/pdf","file_name":"IST-2016-433-v1+1_journal.pone.0085699.pdf","relation":"main_file","access_level":"open_access","file_id":"4646","creator":"system"}]},{"language":[{"iso":"eng"}],"quality_controlled":"1","page":"369 - 383","publisher":"Nature Publishing Group","issue":"6","author":[{"last_name":"Renkawitz","first_name":"Jörg","full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Lademann, Claudio","last_name":"Lademann","first_name":"Claudio"},{"first_name":"Stefan","last_name":"Jentsch","full_name":"Jentsch, Stefan"}],"scopus_import":1,"publication":"Nature Reviews Molecular Cell Biology","_id":"2215","intvolume":"        15","month":"05","title":"Mechanisms and principles of homology search during recombination","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:56:22Z","publication_status":"published","oa_version":"None","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","volume":15,"acknowledgement":"J.R. was supported by a Boehringer Ingelheim Fonds PhD stipend.","type":"journal_article","date_published":"2014-05-14T00:00:00Z","year":"2014","citation":{"short":"J. Renkawitz, C. Lademann, S. Jentsch, Nature Reviews Molecular Cell Biology 15 (2014) 369–383.","mla":"Renkawitz, Jörg, et al. “Mechanisms and Principles of Homology Search during Recombination.” <i>Nature Reviews Molecular Cell Biology</i>, vol. 15, no. 6, Nature Publishing Group, 2014, pp. 369–83, doi:<a href=\"https://doi.org/10.1038/nrm3805\">10.1038/nrm3805</a>.","ista":"Renkawitz J, Lademann C, Jentsch S. 2014. Mechanisms and principles of homology search during recombination. Nature Reviews Molecular Cell Biology. 15(6), 369–383.","ama":"Renkawitz J, Lademann C, Jentsch S. Mechanisms and principles of homology search during recombination. <i>Nature Reviews Molecular Cell Biology</i>. 2014;15(6):369-383. doi:<a href=\"https://doi.org/10.1038/nrm3805\">10.1038/nrm3805</a>","apa":"Renkawitz, J., Lademann, C., &#38; Jentsch, S. (2014). Mechanisms and principles of homology search during recombination. <i>Nature Reviews Molecular Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nrm3805\">https://doi.org/10.1038/nrm3805</a>","ieee":"J. Renkawitz, C. Lademann, and S. Jentsch, “Mechanisms and principles of homology search during recombination,” <i>Nature Reviews Molecular Cell Biology</i>, vol. 15, no. 6. Nature Publishing Group, pp. 369–383, 2014.","chicago":"Renkawitz, Jörg, Claudio Lademann, and Stefan Jentsch. “Mechanisms and Principles of Homology Search during Recombination.” <i>Nature Reviews Molecular Cell Biology</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/nrm3805\">https://doi.org/10.1038/nrm3805</a>."},"date_updated":"2021-01-12T06:56:03Z","publist_id":"4755","abstract":[{"text":"Homologous recombination is crucial for genome stability and for genetic exchange. Although our knowledge of the principle steps in recombination and its machinery is well advanced, homology search, the critical step of exploring the genome for homologous sequences to enable recombination, has remained mostly enigmatic. However, recent methodological advances have provided considerable new insights into this fundamental step in recombination that can be integrated into a mechanistic model. These advances emphasize the importance of genomic proximity and nuclear organization for homology search and the critical role of homology search mediators in this process. They also aid our understanding of how homology search might lead to unwanted and potentially disease-promoting recombination events.","lang":"eng"}],"day":"14","doi":"10.1038/nrm3805"},{"author":[{"full_name":"Dueck, Anne","first_name":"Anne","last_name":"Dueck"},{"first_name":"Alexander","last_name":"Eichner","full_name":"Eichner, Alexander","id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"},{"full_name":"Meister, Gunter","first_name":"Gunter","last_name":"Meister"}],"issue":"4","_id":"2242","publication":"FEBS Letters","scopus_import":1,"month":"02","title":"A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation","intvolume":"       588","publication_status":"published","oa_version":"None","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:56:31Z","language":[{"iso":"eng"}],"page":"632 - 640","quality_controlled":"1","publisher":"Elsevier","date_published":"2014-02-14T00:00:00Z","type":"journal_article","date_updated":"2021-01-12T06:56:14Z","year":"2014","citation":{"chicago":"Dueck, Anne, Alexander Eichner, Michael K Sixt, and Gunter Meister. “A MiR-155-Dependent MicroRNA Hierarchy in Dendritic Cell Maturation and Macrophage Activation.” <i>FEBS Letters</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">https://doi.org/10.1016/j.febslet.2014.01.009</a>.","ieee":"A. Dueck, A. Eichner, M. K. Sixt, and G. Meister, “A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation,” <i>FEBS Letters</i>, vol. 588, no. 4. Elsevier, pp. 632–640, 2014.","ama":"Dueck A, Eichner A, Sixt MK, Meister G. A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation. <i>FEBS Letters</i>. 2014;588(4):632-640. doi:<a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">10.1016/j.febslet.2014.01.009</a>","apa":"Dueck, A., Eichner, A., Sixt, M. K., &#38; Meister, G. (2014). A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation. <i>FEBS Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">https://doi.org/10.1016/j.febslet.2014.01.009</a>","ista":"Dueck A, Eichner A, Sixt MK, Meister G. 2014. A miR-155-dependent microRNA hierarchy in dendritic cell maturation and macrophage activation. FEBS Letters. 588(4), 632–640.","mla":"Dueck, Anne, et al. “A MiR-155-Dependent MicroRNA Hierarchy in Dendritic Cell Maturation and Macrophage Activation.” <i>FEBS Letters</i>, vol. 588, no. 4, Elsevier, 2014, pp. 632–40, doi:<a href=\"https://doi.org/10.1016/j.febslet.2014.01.009\">10.1016/j.febslet.2014.01.009</a>.","short":"A. Dueck, A. Eichner, M.K. Sixt, G. Meister, FEBS Letters 588 (2014) 632–640."},"abstract":[{"lang":"eng","text":"MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR-155-deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence of miR-155 on the expression levels of other miRNAs. For example, miR-455 is negatively regulated in miR-155-deficient cells possibly due to inhibition of the transcription factor C/EBPbeta by miR-155. Based on our comprehensive data sets, we propose a model of hierarchical miRNA expression dominated by miR-155 in DCs and macrophages."}],"publist_id":"4714","doi":"10.1016/j.febslet.2014.01.009","day":"14","publication_identifier":{"issn":["00145793"]},"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","volume":588},{"publisher":"Cell Press","quality_controlled":"1","page":"853 - 854","language":[{"iso":"eng"}],"department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:59:49Z","publication_status":"published","oa_version":"None","intvolume":"        38","month":"05","title":"A conduit to amplify innate immunity","scopus_import":1,"publication":"Immunity","_id":"2830","issue":"5","author":[{"id":"3356F664-F248-11E8-B48F-1D18A9856A87","first_name":"Christine","last_name":"Moussion","full_name":"Moussion, Christine"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"volume":38,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","day":"23","doi":"10.1016/j.immuni.2013.05.005","publist_id":"3969","year":"2013","citation":{"mla":"Moussion, Christine, and Michael K. Sixt. “A Conduit to Amplify Innate Immunity.” <i>Immunity</i>, vol. 38, no. 5, Cell Press, 2013, pp. 853–54, doi:<a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">10.1016/j.immuni.2013.05.005</a>.","short":"C. Moussion, M.K. Sixt, Immunity 38 (2013) 853–854.","ista":"Moussion C, Sixt MK. 2013. A conduit to amplify innate immunity. Immunity. 38(5), 853–854.","apa":"Moussion, C., &#38; Sixt, M. K. (2013). A conduit to amplify innate immunity. <i>Immunity</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">https://doi.org/10.1016/j.immuni.2013.05.005</a>","ama":"Moussion C, Sixt MK. A conduit to amplify innate immunity. <i>Immunity</i>. 2013;38(5):853-854. doi:<a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">10.1016/j.immuni.2013.05.005</a>","ieee":"C. Moussion and M. K. Sixt, “A conduit to amplify innate immunity,” <i>Immunity</i>, vol. 38, no. 5. Cell Press, pp. 853–854, 2013.","chicago":"Moussion, Christine, and Michael K Sixt. “A Conduit to Amplify Innate Immunity.” <i>Immunity</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.immuni.2013.05.005\">https://doi.org/10.1016/j.immuni.2013.05.005</a>."},"date_updated":"2021-01-12T07:00:01Z","type":"journal_article","date_published":"2013-05-23T00:00:00Z"},{"volume":339,"acknowledgement":"We thank M. Frank for technical assistance and S. Cremer, P. Schmalhorst, and E. Kiermaier for critical reading of the manuscript. This work was supported by a Humboldt Foundation postdoctoral fellowship (to M.W.), the German Research Foundation (Si1323 1,2 to M.S.), the Human Frontier Science Program (HFSP RGP0058/2011 to M.S.), the European Research Council (ERC StG 281556 to M.S.), and the Swiss National Science Foundation (31003A 127474 to D.F.L., 130488 to S.A.L.).","date_updated":"2022-06-10T10:21:40Z","year":"2013","citation":{"chicago":"Weber, Michele, Robert Hauschild, Jan Schwarz, Christine Moussion, Ingrid de Vries, Daniel Legler, Sanjiv Luther, Mark Tobias Bollenbach, and Michael K Sixt. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” <i>Science</i>. American Association for the Advancement of Science, 2013. <a href=\"https://doi.org/10.1126/science.1228456\">https://doi.org/10.1126/science.1228456</a>.","ieee":"M. Weber <i>et al.</i>, “Interstitial dendritic cell guidance by haptotactic chemokine gradients,” <i>Science</i>, vol. 339, no. 6117. American Association for the Advancement of Science, pp. 328–332, 2013.","apa":"Weber, M., Hauschild, R., Schwarz, J., Moussion, C., de Vries, I., Legler, D., … Sixt, M. K. (2013). Interstitial dendritic cell guidance by haptotactic chemokine gradients. <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.1228456\">https://doi.org/10.1126/science.1228456</a>","ama":"Weber M, Hauschild R, Schwarz J, et al. Interstitial dendritic cell guidance by haptotactic chemokine gradients. <i>Science</i>. 2013;339(6117):328-332. doi:<a href=\"https://doi.org/10.1126/science.1228456\">10.1126/science.1228456</a>","ista":"Weber M, Hauschild R, Schwarz J, Moussion C, de Vries I, Legler D, Luther S, Bollenbach MT, Sixt MK. 2013. Interstitial dendritic cell guidance by haptotactic chemokine gradients. Science. 339(6117), 328–332.","short":"M. Weber, R. Hauschild, J. Schwarz, C. Moussion, I. de Vries, D. Legler, S. Luther, M.T. Bollenbach, M.K. Sixt, Science 339 (2013) 328–332.","mla":"Weber, Michele, et al. “Interstitial Dendritic Cell Guidance by Haptotactic Chemokine Gradients.” <i>Science</i>, vol. 339, no. 6117, American Association for the Advancement of Science, 2013, pp. 328–32, doi:<a href=\"https://doi.org/10.1126/science.1228456\">10.1126/science.1228456</a>."},"doi":"10.1126/science.1228456","day":"18","abstract":[{"lang":"eng","text":"Directional guidance of cells via gradients of chemokines is considered crucial for embryonic development, cancer dissemination, and immune responses. Nevertheless, the concept still lacks direct experimental confirmation in vivo. Here, we identify endogenous gradients of the chemokine CCL21 within mouse skin and show that they guide dendritic cells toward lymphatic vessels. Quantitative imaging reveals depots of CCL21 within lymphatic endothelial cells and steeply decaying gradients within the perilymphatic interstitium. These gradients match the migratory patterns of the dendritic cells, which directionally approach vessels from a distance of up to 90-micrometers. Interstitial CCL21 is immobilized to heparan sulfates, and its experimental delocalization or swamping the endogenous gradients abolishes directed migration. These findings functionally establish the concept of haptotaxis, directed migration along immobilized gradients, in tissues."}],"page":"328 - 332","quality_controlled":"1","ec_funded":1,"publisher":"American Association for the Advancement of Science","article_type":"original","_id":"2839","scopus_import":"1","author":[{"full_name":"Weber, Michele","last_name":"Weber","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","last_name":"Hauschild","first_name":"Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schwarz, Jan","first_name":"Jan","last_name":"Schwarz","id":"346C1EC6-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Christine","last_name":"Moussion","full_name":"Moussion, Christine","id":"3356F664-F248-11E8-B48F-1D18A9856A87"},{"full_name":"De Vries, Ingrid","last_name":"De Vries","first_name":"Ingrid","id":"4C7D837E-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Legler","first_name":"Daniel","full_name":"Legler, Daniel"},{"last_name":"Luther","first_name":"Sanjiv","full_name":"Luther, Sanjiv"},{"full_name":"Bollenbach, Mark Tobias","orcid":"0000-0003-4398-476X","last_name":"Bollenbach","first_name":"Mark Tobias","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K"}],"issue":"6117","publication_status":"published","department":[{"_id":"MiSi"},{"_id":"Bio"}],"date_created":"2018-12-11T11:59:52Z","article_processing_charge":"No","title":"Interstitial dendritic cell guidance by haptotactic chemokine gradients","intvolume":"       339","main_file_link":[{"open_access":"1","url":"https://kops.uni-konstanz.de/bitstream/123456789/26341/2/Weber_263418.pdf"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","date_published":"2013-01-18T00:00:00Z","type":"journal_article","oa":1,"publist_id":"3959","language":[{"iso":"eng"}],"publication":"Science","oa_version":"Published Version","project":[{"_id":"25A603A2-B435-11E9-9278-68D0E5697425","call_identifier":"FP7","name":"Cytoskeletal force generation and force transduction of migrating leukocytes (EU)","grant_number":"281556"},{"grant_number":"RGP0058/2011","name":"Cell migration in complex environments: from in vivo experiments to theoretical models","_id":"25ABD200-B435-11E9-9278-68D0E5697425"}],"month":"01"},{"acknowledgement":"We would like to thank Alexander Eichner and Ingrid de Vries for discussion and critical reading of the manuscript, and Mary Frank for assistance with the recording of videos and images in Fig. 1. M.S. is supported through funding from the German Research Foundation (DFG). M.W. acknowledges the Alexander von Humboldt Foundation for funding.","volume":1013,"external_id":{"pmid":["23625502"]},"year":"2013","citation":{"ista":"Weber M, Sixt MK. 2013.Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In: Chemokines. Methods in Molecular Biology, vol. 1013, 215–226.","short":"M. Weber, M.K. Sixt, in:, A. Cardona, E. Ubogu (Eds.), Chemokines, Humana Press, Totowa, NJ, 2013, pp. 215–226.","mla":"Weber, Michele, and Michael K. Sixt. “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations.” <i>Chemokines</i>, edited by Astrid Cardona and Eroboghene Ubogu, vol. 1013, Humana Press, 2013, pp. 215–26, doi:<a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">10.1007/978-1-62703-426-5_14</a>.","chicago":"Weber, Michele, and Michael K Sixt. “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations.” In <i>Chemokines</i>, edited by Astrid Cardona and Eroboghene Ubogu, 1013:215–26. MIMB. Totowa, NJ: Humana Press, 2013. <a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">https://doi.org/10.1007/978-1-62703-426-5_14</a>.","ieee":"M. Weber and M. K. Sixt, “Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations,” in <i>Chemokines</i>, vol. 1013, A. Cardona and E. Ubogu, Eds. Totowa, NJ: Humana Press, 2013, pp. 215–226.","apa":"Weber, M., &#38; Sixt, M. K. (2013). Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In A. Cardona &#38; E. Ubogu (Eds.), <i>Chemokines</i> (Vol. 1013, pp. 215–226). Totowa, NJ: Humana Press. <a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">https://doi.org/10.1007/978-1-62703-426-5_14</a>","ama":"Weber M, Sixt MK. Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations. In: Cardona A, Ubogu E, eds. <i>Chemokines</i>. Vol 1013. MIMB. Totowa, NJ: Humana Press; 2013:215-226. doi:<a href=\"https://doi.org/10.1007/978-1-62703-426-5_14\">10.1007/978-1-62703-426-5_14</a>"},"date_updated":"2023-09-05T13:15:33Z","abstract":[{"lang":"eng","text":"Leukocyte migration through the interstitial space is crucial for the maintenance of tolerance and immunity. The main cues for leukocyte trafficking are chemokines thought to directionally guide these cells towards their targets. However, model systems that facilitate quantification of chemokine-guided leukocyte migration in vivo are uncommon. Here we describe an ex vivo crawl-in assay using explanted mouse ears that allows the visualization of chemokine-dependent dendritic cell (DC) motility in the dermal interstitium in real time. We present methods for the preparation of mouse ear sheets and their use in multidimensional confocal imaging experiments to monitor and analyze the directional migration of fluorescently labelled DCs through the dermis and into afferent lymphatic vessels. The assay provides a more physiological approach to study leukocyte migration than in vitro three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen gels and transwell assays."}],"day":"03","doi":"10.1007/978-1-62703-426-5_14","series_title":"MIMB","quality_controlled":"1","page":"215-226","editor":[{"full_name":"Cardona, Astrid","last_name":"Cardona","first_name":"Astrid"},{"full_name":"Ubogu, Eroboghene","last_name":"Ubogu","first_name":"Eroboghene"}],"publisher":"Humana Press","author":[{"full_name":"Weber, Michele","last_name":"Weber","first_name":"Michele","id":"3A3FC708-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K"}],"scopus_import":"1","_id":"10900","pmid":1,"intvolume":"      1013","title":"Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations","alternative_title":["Methods in Molecular Biology"],"department":[{"_id":"MiSi"}],"article_processing_charge":"No","date_created":"2022-03-21T07:47:41Z","publication_status":"published","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","place":"Totowa, NJ","type":"book_chapter","date_published":"2013-04-03T00:00:00Z","publication_identifier":{"isbn":["9781627034258"],"eisbn":["9781627034265"],"issn":["1064-3745"],"eissn":["1940-6029"]},"language":[{"iso":"eng"}],"publication":"Chemokines","month":"04","oa_version":"None"},{"date_updated":"2021-01-12T08:01:22Z","citation":{"ieee":"E. Fuertbauer <i>et al.</i>, “Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells,” <i>Immunology Letters</i>, vol. 154, no. 1–2. Elsevier, pp. 31–41, 2013.","chicago":"Fuertbauer, Elke, Jan Zaujec, Pavel Uhrin, Ingrid Raab, Michele Weber, Helga Schachner, Miroslav Bauer, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes Natural Regulatory T Cells.” <i>Immunology Letters</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">https://doi.org/10.1016/j.imlet.2013.07.007</a>.","apa":"Fuertbauer, E., Zaujec, J., Uhrin, P., Raab, I., Weber, M., Schachner, H., … Stockinger, H. (2013). Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. <i>Immunology Letters</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">https://doi.org/10.1016/j.imlet.2013.07.007</a>","ama":"Fuertbauer E, Zaujec J, Uhrin P, et al. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. <i>Immunology Letters</i>. 2013;154(1-2):31-41. doi:<a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">10.1016/j.imlet.2013.07.007</a>","ista":"Fuertbauer E, Zaujec J, Uhrin P, Raab I, Weber M, Schachner H, Bauer M, Schütz G, Binder B, Sixt MK, Kerjaschki D, Stockinger H. 2013. Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells. Immunology Letters. 154(1–2), 31–41.","mla":"Fuertbauer, Elke, et al. “Thymic Medullar Conduits-Associated Podoplanin Promotes Natural Regulatory T Cells.” <i>Immunology Letters</i>, vol. 154, no. 1–2, Elsevier, 2013, pp. 31–41, doi:<a href=\"https://doi.org/10.1016/j.imlet.2013.07.007\">10.1016/j.imlet.2013.07.007</a>.","short":"E. Fuertbauer, J. Zaujec, P. Uhrin, I. Raab, M. Weber, H. Schachner, M. Bauer, G. Schütz, B. Binder, M.K. Sixt, D. Kerjaschki, H. Stockinger, Immunology Letters 154 (2013) 31–41."},"year":"2013","date_published":"2013-07-01T00:00:00Z","type":"journal_article","doi":"10.1016/j.imlet.2013.07.007","day":"01","abstract":[{"lang":"eng","text":"Podoplanin, a mucin-like plasma membrane protein, is expressed by lymphatic endothelial cells and responsible for separation of blood and lymphatic circulation through activation of platelets. Here we show that podoplanin is also expressed by thymic fibroblastic reticular cells (tFRC), a novel thymic medulla stroma cell type associated with thymic conduits, and involved in development of natural regulatory T cells (nTreg). Young mice deficient in podoplanin lack nTreg owing to retardation of CD4+CD25+ thymocytes in the cortex and missing differentiation of Foxp3+ thymocytes in the medulla. This might be due to CCL21 that delocalizes upon deletion of the CCL21-binding podoplanin from medullar tFRC to cortex areas. The animals do not remain devoid of nTreg but generate them delayed within the first month resulting in Th2-biased hypergammaglobulinemia but not in the death-causing autoimmune phenotype of Foxp3-deficient Scurfy mice."}],"publist_id":"7300","volume":154,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","publication":"Immunology Letters","_id":"522","scopus_import":1,"author":[{"last_name":"Fuertbauer","first_name":"Elke","full_name":"Fuertbauer, Elke"},{"last_name":"Zaujec","first_name":"Jan","full_name":"Zaujec, Jan"},{"first_name":"Pavel","last_name":"Uhrin","full_name":"Uhrin, Pavel"},{"full_name":"Raab, Ingrid","first_name":"Ingrid","last_name":"Raab"},{"id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","full_name":"Weber, Michele","last_name":"Weber","first_name":"Michele"},{"first_name":"Helga","last_name":"Schachner","full_name":"Schachner, Helga"},{"first_name":"Miroslav","last_name":"Bauer","full_name":"Bauer, Miroslav"},{"full_name":"Schütz, Gerhard","first_name":"Gerhard","last_name":"Schütz"},{"last_name":"Binder","first_name":"Bernd","full_name":"Binder, Bernd"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"},{"full_name":"Kerjaschki, Dontscho","last_name":"Kerjaschki","first_name":"Dontscho"},{"last_name":"Stockinger","first_name":"Hannes","full_name":"Stockinger, Hannes"}],"issue":"1-2","oa_version":"None","publication_status":"published","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:46:57Z","title":"Thymic medullar conduits-associated podoplanin promotes natural regulatory T cells","month":"07","intvolume":"       154","page":"31 - 41","quality_controlled":"1","language":[{"iso":"eng"}],"publisher":"Elsevier"},{"status":"public","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","volume":12,"acknowledgement":"We thank M. Sixt and A. Peixoto for helpful comments on the manuscript. Work in the laboratory of J.-P.G. is supported by grants from Fondation ARC pour la Recherche sur le Cancer, Agence Nationale de la Recherche (ANR), Institut National du Cancer (INCA), Fondation RITC and Région Midi-Pyrénées. Research by R.F. is supported by Deutsche Forschungsgemeinschaft (DFG) grants SFB621-A1, SFB738-B5, SFB587-B3, SFB900-B1 and KFO 250-FO 334/2-1. We regret that, owing to space limitations, we could not always quote the work of colleagues who have contributed to the field.","date_published":"2012-11-01T00:00:00Z","type":"journal_article","date_updated":"2021-01-12T07:39:57Z","year":"2012","citation":{"short":"J. Girard, C. Moussion, R. Förster, Nature Reviews Immunology 12 (2012) 762–773.","mla":"Girard, Jean, et al. “HEVs, Lymphatics and Homeostatic Immune Cell Trafficking in Lymph Nodes.” <i>Nature Reviews Immunology</i>, vol. 12, no. 11, Nature Publishing Group, 2012, pp. 762–73, doi:<a href=\"https://doi.org/10.1038/nri3298\">10.1038/nri3298</a>.","ista":"Girard J, Moussion C, Förster R. 2012. HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. Nature Reviews Immunology. 12(11), 762–773.","apa":"Girard, J., Moussion, C., &#38; Förster, R. (2012). HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. <i>Nature Reviews Immunology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nri3298\">https://doi.org/10.1038/nri3298</a>","ama":"Girard J, Moussion C, Förster R. HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes. <i>Nature Reviews Immunology</i>. 2012;12(11):762-773. doi:<a href=\"https://doi.org/10.1038/nri3298\">10.1038/nri3298</a>","chicago":"Girard, Jean, Christine Moussion, and Reinhold Förster. “HEVs, Lymphatics and Homeostatic Immune Cell Trafficking in Lymph Nodes.” <i>Nature Reviews Immunology</i>. Nature Publishing Group, 2012. <a href=\"https://doi.org/10.1038/nri3298\">https://doi.org/10.1038/nri3298</a>.","ieee":"J. Girard, C. Moussion, and R. Förster, “HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes,” <i>Nature Reviews Immunology</i>, vol. 12, no. 11. Nature Publishing Group, pp. 762–773, 2012."},"abstract":[{"lang":"eng","text":"In search of foreign antigens, lymphocytes recirculate from the blood, through lymph nodes, into lymphatics and back to the blood. Dendritic cells also migrate to lymph nodes for optimal interaction with lymphocytes. This continuous trafficking of immune cells into and out of lymph nodes is essential for immune surveillance of foreign invaders. In this article, we review our current understanding of the functions of high endothelial venules (HEVs), stroma and lymphatics in the entry, positioning and exit of immune cells in lymph nodes during homeostasis, and we highlight the unexpected role of dendritic cells in the control of lymphocyte homing through HEVs."}],"publist_id":"3787","doi":"10.1038/nri3298","day":"01","language":[{"iso":"eng"}],"page":"762 - 773","quality_controlled":"1","publisher":"Nature Publishing Group","author":[{"full_name":"Girard, Jean","first_name":"Jean","last_name":"Girard"},{"id":"3356F664-F248-11E8-B48F-1D18A9856A87","full_name":"Moussion, Christine","first_name":"Christine","last_name":"Moussion"},{"first_name":"Reinhold","last_name":"Förster","full_name":"Förster, Reinhold"}],"issue":"11","publication":"Nature Reviews Immunology","_id":"2945","scopus_import":1,"title":"HEVs, lymphatics and homeostatic immune cell trafficking in lymph nodes","month":"11","intvolume":"        12","publication_status":"published","oa_version":"None","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T12:00:29Z"},{"language":[{"iso":"eng"}],"publication":"Nucleic Acids Research","has_accepted_license":"1","month":"10","oa_version":"Published Version","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","file":[{"date_updated":"2020-07-14T12:45:55Z","file_name":"IST-2015-383-v1+1_Nucl._Acids_Res.-2012-Dueck-9850-62.pdf","content_type":"application/pdf","date_created":"2018-12-12T10:13:12Z","file_size":8126936,"checksum":"1bb8d1ff894014b481657a21083c941c","file_id":"4993","creator":"system","relation":"main_file","access_level":"open_access"}],"date_published":"2012-10-01T00:00:00Z","type":"journal_article","tmp":{"name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","image":"/images/cc_by_nc.png","short":"CC BY-NC (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode"},"oa":1,"publist_id":"3786","file_date_updated":"2020-07-14T12:45:55Z","page":"9850 - 9862","quality_controlled":"1","publisher":"Oxford University Press","author":[{"first_name":"Anne","last_name":"Dueck","full_name":"Dueck, Anne"},{"full_name":"Ziegler, Christian","first_name":"Christian","last_name":"Ziegler"},{"id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","full_name":"Eichner, Alexander","last_name":"Eichner","first_name":"Alexander"},{"full_name":"Berezikov, Eugène","first_name":"Eugène","last_name":"Berezikov"},{"full_name":"Meister, Gunter","last_name":"Meister","first_name":"Gunter"}],"issue":"19","_id":"2946","scopus_import":1,"license":"https://creativecommons.org/licenses/by-nc/4.0/","title":"MicroRNAs associated with the different human Argonaute proteins","pubrep_id":"383","intvolume":"        40","publication_status":"published","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T12:00:29Z","ddc":["570"],"volume":40,"acknowledgement":"Deutsche Forschungsgemeinschaft (DFG) (SFB 960 and FOR855); European Research Council (ERC grant ‘sRNAs’); European Union (FP7 project ‘ONCOMIRs’); German Bundesministerium für Bildung und Forschung (BMBF, NGFN+, FKZ PIM-01GS0804-5); Bavarian Genome Research Network (BayGene to G.M.); The Netherlands Organization for Scientific Research (NWO, VIDI grant to E.B.). Funding for open access charge: DFG via the open access publishing program. \r\n\r\nWe thank Sigrun Ammon and Corinna Friederich for technical assistance and Sebastian Petri and Daniel Schraivogel for helpful discussions.","date_updated":"2021-01-12T07:39:57Z","citation":{"ista":"Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. 2012. MicroRNAs associated with the different human Argonaute proteins. Nucleic Acids Research. 40(19), 9850–9862.","short":"A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, G. Meister, Nucleic Acids Research 40 (2012) 9850–9862.","mla":"Dueck, Anne, et al. “MicroRNAs Associated with the Different Human Argonaute Proteins.” <i>Nucleic Acids Research</i>, vol. 40, no. 19, Oxford University Press, 2012, pp. 9850–62, doi:<a href=\"https://doi.org/10.1093/nar/gks705\">10.1093/nar/gks705</a>.","chicago":"Dueck, Anne, Christian Ziegler, Alexander Eichner, Eugène Berezikov, and Gunter Meister. “MicroRNAs Associated with the Different Human Argonaute Proteins.” <i>Nucleic Acids Research</i>. Oxford University Press, 2012. <a href=\"https://doi.org/10.1093/nar/gks705\">https://doi.org/10.1093/nar/gks705</a>.","ieee":"A. Dueck, C. Ziegler, A. Eichner, E. Berezikov, and G. Meister, “MicroRNAs associated with the different human Argonaute proteins,” <i>Nucleic Acids Research</i>, vol. 40, no. 19. Oxford University Press, pp. 9850–9862, 2012.","apa":"Dueck, A., Ziegler, C., Eichner, A., Berezikov, E., &#38; Meister, G. (2012). MicroRNAs associated with the different human Argonaute proteins. <i>Nucleic Acids Research</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/nar/gks705\">https://doi.org/10.1093/nar/gks705</a>","ama":"Dueck A, Ziegler C, Eichner A, Berezikov E, Meister G. MicroRNAs associated with the different human Argonaute proteins. <i>Nucleic Acids Research</i>. 2012;40(19):9850-9862. doi:<a href=\"https://doi.org/10.1093/nar/gks705\">10.1093/nar/gks705</a>"},"year":"2012","abstract":[{"text":"MicroRNAs (miRNAs) are small noncoding RNAs that function in literally all cellular processes. miRNAs interact with Argonaute (Ago) proteins and guide them to specific target sites located in the 3′-untranslated region (3′-UTR) of target mRNAs leading to translational repression and deadenylation-induced mRNA degradation. Most miRNAs are processed from hairpin-structured precursors by the consecutive action of the RNase III enzymes Drosha and Dicer. However, processing of miR-451 is Dicer independent and cleavage is mediated by the endonuclease Ago2. Here we have characterized miR-451 sequence and structure requirements for processing as well as sorting of miRNAs into different Ago proteins. Pre-miR-451 appears to be optimized for Ago2 cleavage and changes result in reduced processing. In addition, we show that the mature miR-451 only associates with Ago2 suggesting that mature miRNAs are not exchanged between different members of the Ago protein family. Based on cloning and deep sequencing of endogenous miRNAs associated with Ago1-3, we do not find evidence for miRNA sorting in human cells. However, Ago identity appears to influence the length of some miRNAs, while others remain unaffected.","lang":"eng"}],"doi":"10.1093/nar/gks705","day":"01"},{"publication":"European Journal of Cell Biology","month":"11","oa_version":"Submitted Version","language":[{"iso":"eng"}],"date_published":"2012-11-01T00:00:00Z","type":"journal_article","publist_id":"3534","oa":1,"user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","status":"public","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3930012/"}],"author":[{"first_name":"Hannah","last_name":"Schachtner","full_name":"Schachtner, Hannah"},{"full_name":"Li, Ang","last_name":"Li","first_name":"Ang"},{"full_name":"Stevenson, David","first_name":"David","last_name":"Stevenson"},{"full_name":"Calaminus, Simon","first_name":"Simon","last_name":"Calaminus"},{"last_name":"Thomas","first_name":"Steven","full_name":"Thomas, Steven"},{"last_name":"Watson","first_name":"Steve","full_name":"Watson, Steve"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"},{"last_name":"Wedlich Söldner","first_name":"Roland","full_name":"Wedlich Söldner, Roland"},{"full_name":"Strathdee, Douglas","last_name":"Strathdee","first_name":"Douglas"},{"last_name":"Machesky","first_name":"Laura","full_name":"Machesky, Laura"}],"issue":"11-12","pmid":1,"_id":"3158","scopus_import":1,"title":"Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo","intvolume":"        91","publication_status":"published","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T12:01:44Z","page":"923 - 929","quality_controlled":"1","publisher":"Elsevier","external_id":{"pmid":["22658956"]},"date_updated":"2021-01-12T07:41:27Z","citation":{"ama":"Schachtner H, Li A, Stevenson D, et al. Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. <i>European Journal of Cell Biology</i>. 2012;91(11-12):923-929. doi:<a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">10.1016/j.ejcb.2012.04.002</a>","apa":"Schachtner, H., Li, A., Stevenson, D., Calaminus, S., Thomas, S., Watson, S., … Machesky, L. (2012). Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. <i>European Journal of Cell Biology</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">https://doi.org/10.1016/j.ejcb.2012.04.002</a>","ieee":"H. Schachtner <i>et al.</i>, “Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo,” <i>European Journal of Cell Biology</i>, vol. 91, no. 11–12. Elsevier, pp. 923–929, 2012.","chicago":"Schachtner, Hannah, Ang Li, David Stevenson, Simon Calaminus, Steven Thomas, Steve Watson, Michael K Sixt, Roland Wedlich Söldner, Douglas Strathdee, and Laura Machesky. “Tissue Inducible Lifeact Expression Allows Visualization of Actin Dynamics in Vivo and Ex Vivo.” <i>European Journal of Cell Biology</i>. Elsevier, 2012. <a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">https://doi.org/10.1016/j.ejcb.2012.04.002</a>.","short":"H. Schachtner, A. Li, D. Stevenson, S. Calaminus, S. Thomas, S. Watson, M.K. Sixt, R. Wedlich Söldner, D. Strathdee, L. Machesky, European Journal of Cell Biology 91 (2012) 923–929.","mla":"Schachtner, Hannah, et al. “Tissue Inducible Lifeact Expression Allows Visualization of Actin Dynamics in Vivo and Ex Vivo.” <i>European Journal of Cell Biology</i>, vol. 91, no. 11–12, Elsevier, 2012, pp. 923–29, doi:<a href=\"https://doi.org/10.1016/j.ejcb.2012.04.002\">10.1016/j.ejcb.2012.04.002</a>.","ista":"Schachtner H, Li A, Stevenson D, Calaminus S, Thomas S, Watson S, Sixt MK, Wedlich Söldner R, Strathdee D, Machesky L. 2012. Tissue inducible Lifeact expression allows visualization of actin dynamics in vivo and ex vivo. European Journal of Cell Biology. 91(11–12), 923–929."},"year":"2012","abstract":[{"text":"We describe here the development and characterization of a conditionally inducible mouse model expressing Lifeact-GFP, a peptide that reports the dynamics of filamentous actin. We have used this model to study platelets, megakaryocytes and melanoblasts and we provide evidence that Lifeact-GFP is a useful reporter in these cell types ex vivo. In the case of platelets and megakaryocytes, these cells are not transfectable by traditional methods, so conditional activation of Lifeact allows the study of actin dynamics in these cells live. We studied melanoblasts in native skin explants from embryos, allowing the visualization of live actin dynamics during cytokinesis and migration. Our study revealed that melanoblasts lacking the small GTPase Rac1 show a delay in the formation of new pseudopodia following cytokinesis that accounts for the previously reported cytokinesis delay in these cells. Thus, through use of this mouse model, we were able to gain insights into the actin dynamics of cells that could only previously be studied using fixed specimens or following isolation from their native tissue environment.","lang":"eng"}],"doi":"10.1016/j.ejcb.2012.04.002","day":"01","volume":91},{"page":"32-34","language":[{"iso":"eng"}],"publisher":"American Association for the Advancement of Science","article_type":"letter_note","_id":"3167","pmid":1,"publication":"Science","author":[{"id":"3A3FC708-F248-11E8-B48F-1D18A9856A87","last_name":"Weber","first_name":"Michele","full_name":"Weber, Michele"}],"issue":"6077","oa_version":"None","publication_status":"published","date_created":"2018-12-11T12:01:47Z","department":[{"_id":"MiSi"}],"title":"NextGen speaks 13 ","month":"04","intvolume":"       336","volume":336,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","date_updated":"2021-01-12T07:41:32Z","citation":{"ista":"Weber M. 2012. NextGen speaks 13 . Science. 336(6077), 32–34.","mla":"Weber, Michele. “NextGen Speaks 13 .” <i>Science</i>, vol. 336, no. 6077, American Association for the Advancement of Science, 2012, pp. 32–34, doi:<a href=\"https://doi.org/10.1126/science.336.6077.32\">10.1126/science.336.6077.32</a>.","short":"M. Weber, Science 336 (2012) 32–34.","chicago":"Weber, Michele. “NextGen Speaks 13 .” <i>Science</i>. American Association for the Advancement of Science, 2012. <a href=\"https://doi.org/10.1126/science.336.6077.32\">https://doi.org/10.1126/science.336.6077.32</a>.","ieee":"M. Weber, “NextGen speaks 13 ,” <i>Science</i>, vol. 336, no. 6077. American Association for the Advancement of Science, pp. 32–34, 2012.","apa":"Weber, M. (2012). NextGen speaks 13 . <i>Science</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/science.336.6077.32\">https://doi.org/10.1126/science.336.6077.32</a>","ama":"Weber M. NextGen speaks 13 . <i>Science</i>. 2012;336(6077):32-34. doi:<a href=\"https://doi.org/10.1126/science.336.6077.32\">10.1126/science.336.6077.32</a>"},"year":"2012","popular_science":"1","date_published":"2012-04-06T00:00:00Z","type":"journal_article","external_id":{"pmid":["22491839"]},"doi":"10.1126/science.336.6077.32","day":"06","publist_id":"3516"},{"file":[{"date_created":"2019-02-12T09:03:09Z","checksum":"45c02be33ebd99fc3077d60b9c90bdfa","file_size":986566,"date_updated":"2020-07-14T12:46:36Z","file_name":"2012_CellBiology_Sixt.pdf","content_type":"application/pdf","relation":"main_file","access_level":"open_access","file_id":"5957","creator":"kschuh"}],"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publist_id":"7314","oa":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","image":"/images/cc_by_nc_sa.png","short":"CC BY-NC-SA (4.0)"},"date_published":"2012-04-30T00:00:00Z","type":"journal_article","language":[{"iso":"eng"}],"oa_version":"Published Version","month":"04","publication":"Journal of Cell Biology","has_accepted_license":"1","volume":197,"ddc":["570"],"doi":"10.1083/jcb.201204039","day":"30","date_updated":"2021-01-12T08:01:11Z","citation":{"short":"M.K. Sixt, Journal of Cell Biology 197 (2012) 347–349.","mla":"Sixt, Michael K. “Cell Migration: Fibroblasts Find a New Way to Get Ahead.” <i>Journal of Cell Biology</i>, vol. 197, no. 3, Rockefeller University Press, 2012, pp. 347–49, doi:<a href=\"https://doi.org/10.1083/jcb.201204039\">10.1083/jcb.201204039</a>.","ista":"Sixt MK. 2012. Cell migration: Fibroblasts find a new way to get ahead. Journal of Cell Biology. 197(3), 347–349.","apa":"Sixt, M. K. (2012). Cell migration: Fibroblasts find a new way to get ahead. <i>Journal of Cell Biology</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1083/jcb.201204039\">https://doi.org/10.1083/jcb.201204039</a>","ama":"Sixt MK. Cell migration: Fibroblasts find a new way to get ahead. <i>Journal of Cell Biology</i>. 2012;197(3):347-349. doi:<a href=\"https://doi.org/10.1083/jcb.201204039\">10.1083/jcb.201204039</a>","chicago":"Sixt, Michael K. “Cell Migration: Fibroblasts Find a New Way to Get Ahead.” <i>Journal of Cell Biology</i>. Rockefeller University Press, 2012. <a href=\"https://doi.org/10.1083/jcb.201204039\">https://doi.org/10.1083/jcb.201204039</a>.","ieee":"M. K. Sixt, “Cell migration: Fibroblasts find a new way to get ahead,” <i>Journal of Cell Biology</i>, vol. 197, no. 3. Rockefeller University Press, pp. 347–349, 2012."},"year":"2012","publisher":"Rockefeller University Press","article_type":"original","page":"347 - 349","quality_controlled":"1","file_date_updated":"2020-07-14T12:46:36Z","publication_status":"published","date_created":"2018-12-11T11:46:51Z","department":[{"_id":"MiSi"}],"article_processing_charge":"No","title":"Cell migration: Fibroblasts find a new way to get ahead","intvolume":"       197","_id":"506","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","scopus_import":1,"author":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"}],"issue":"3"},{"month":"03","oa_version":"Published Version","has_accepted_license":"1","language":[{"iso":"eng"}],"publist_id":"3371","oa":1,"supervisor":[{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K","first_name":"Michael K","last_name":"Sixt"}],"publication_identifier":{"issn":["2663-337X"]},"type":"dissertation","date_published":"2011-03-01T00:00:00Z","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","status":"public","file":[{"date_created":"2019-03-26T08:12:21Z","checksum":"e69eee6252660f0b694a2ea8923ddc72","file_size":4487708,"date_updated":"2020-07-14T12:46:06Z","content_type":"application/pdf","file_name":"2011_Thesis_Kathrin_Schumann.pdf","access_level":"closed","relation":"main_file","file_id":"6177","creator":"dernst"},{"file_name":"2011_Thesis_Schumann_noS.pdf","content_type":"application/pdf","date_updated":"2021-02-22T11:24:30Z","checksum":"71727d63f424b5b446f68f4b87ecadc0","file_size":4313127,"date_created":"2021-02-22T11:24:30Z","creator":"dernst","file_id":"9175","relation":"main_file","success":1,"access_level":"open_access"}],"alternative_title":["ISTA Thesis"],"pubrep_id":"11","title":"The role of chemotactic gradients in dendritic cell migration","article_processing_charge":"No","department":[{"_id":"MiSi"}],"date_created":"2018-12-11T12:02:24Z","publication_status":"published","author":[{"full_name":"Schumann, Kathrin","first_name":"Kathrin","last_name":"Schumann","id":"F44D762E-4F9D-11E9-B64C-9EB26CEFFB5F"}],"_id":"3275","publisher":"Institute of Science and Technology Austria","file_date_updated":"2021-02-22T11:24:30Z","page":"141","abstract":[{"text":"Chemokines organize immune cell trafficking by inducing either directed (tactic) or random (kinetic) migration and by activating integrins in order to support surface adhesion (haptic). Beyond that the same chemokines can establish clearly defined functional areas in secondary lymphoid organs. Until now it is unclear how chemokines can fulfill such diverse functions. One decisive prerequisite to explain these capacities is to know how chemokines are presented in tissue. In theory chemokines could occur either soluble or immobilized, and could be distributed either homogenously or as a concentration gradient. To dissect if and how the presenting mode of chemokines influences immune cells, I tested the response of dendritic cells (DCs) to differentially displayed chemokines. DCs are antigen presenting cells that reside in the periphery and migrate into draining lymph nodes (LNs) once exposed to inflammatory stimuli to activate naïve T cells. DCs are guided to and within the LN by the chemokine receptor CCR7, which has two ligands, the chemokines CCL19 and CCL21. Both CCR7 ligands are expressed by fibroblastic reticular cells in the LN, but differ in their ability to bind to heparan sulfate residues. CCL21 has a highly charged C-terminal extension, which mediates binding to anionic surfaces, whereas CCL19 is lacking such residues and likely distributes as a soluble molecule. This study shows that surface-bound CCL21 causes random, haptokinetic DC motility, which is confined to the chemokine coated area by insideout activation of β2 integrins that mediate cell binding to the surface. CCL19 on the other hand forms concentration gradients which trigger directional, chemotactic movement, but no surface adhesion. In addition DCs can actively manipulate this system by recruiting and activating serine proteases on their surfaces, which create - by proteolytically removing the adhesive C-terminus - a solubilized variant of CCL21 that functionally resembles CCL19. By generating a CCL21 concentration gradient DCs establish a positive feedback loop to recruit further DCs from the periphery to the CCL21 coated region. In addition DCs can sense chemotactic gradients as well as immobilized haptokinetic fields at the same time and integrate these signals. The result is chemotactically biased haptokinesis - directional migration confined to a chemokine coated track or area - which could explain the dynamic but spatially tightly controlled swarming leukocyte locomotion patterns that have been observed in lymphatic organs by intravital microscopists. The finding that DCs can approach soluble cues in a non-adhesive manner while they attach to surfaces coated with immobilized cues raises the question how these cells transmit intracellular forces to the environment, especially in the non-adherent migration mode. In order to migrate, cells have to generate and transmit force to the extracellular substrate. Force transmission is the prerequisite to procure an expansion of the leading edge and a forward motion of the whole cell body. In the current conceptions actin polymerization at the leading edge is coupled to extracellular ligands via the integrin family of transmembrane receptors, which allows the transmission of intracellular force. Against the paradigm of force transmission during migration, leukocytes, like DCs, are able to migrate in threedimensional environments without using integrin transmembrane receptors (Lämmermann et al., 2008). This reflects the biological function of leukocytes, as they can invade almost all tissues, whereby their migration has to be independent from the extracellular environment. How the cells can achieve this is unclear. For this study I examined DC migration in a defined threedimensional environment and highlighted actin-dynamics with the probe Lifeact-GFP. The result was that chemotactic DCs can switch between integrin-dependent and integrin- independent locomotion and can thereby adapt to the adhesive properties of their environment. If the cells are able to couple their actin cytoskeleton to the substrate, actin polymerization is entirely converted into protrusion. Without coupling the actin cortex undergoes slippage and retrograde actin flow can be observed. But retrograde actin flow can be completely compensated by higher actin polymerization rate keeping the migration velocity and the shape of the cells unaltered. Mesenchymal cells like fibroblast cannot balance the loss of adhesive interaction, cannot protrude into open space and, therefore, strictly depend on integrinmediated force coupling. This leukocyte specific phenomenon of “adaptive force transmission” endows these cells with the unique ability to transit and invade almost every type of tissue. ","lang":"eng"}],"day":"01","degree_awarded":"PhD","year":"2011","citation":{"ieee":"K. Schumann, “The role of chemotactic gradients in dendritic cell migration,” Institute of Science and Technology Austria, 2011.","chicago":"Schumann, Kathrin. “The Role of Chemotactic Gradients in Dendritic Cell Migration.” Institute of Science and Technology Austria, 2011.","ama":"Schumann K. The role of chemotactic gradients in dendritic cell migration. 2011.","apa":"Schumann, K. (2011). <i>The role of chemotactic gradients in dendritic cell migration</i>. Institute of Science and Technology Austria.","ista":"Schumann K. 2011. The role of chemotactic gradients in dendritic cell migration. Institute of Science and Technology Austria.","short":"K. Schumann, The Role of Chemotactic Gradients in Dendritic Cell Migration, Institute of Science and Technology Austria, 2011.","mla":"Schumann, Kathrin. <i>The Role of Chemotactic Gradients in Dendritic Cell Migration</i>. Institute of Science and Technology Austria, 2011."},"date_updated":"2023-09-07T11:31:48Z","ddc":["570","579"],"acknowledgement":"I would like to express my sincere gratitude to the following people who made with their continuous support and encouragement this thesis possible: First, I want to thank Prof. Dr. Michael Sixt for his excellent supervision and mentoring, especially for the nice, relaxed working atmosphere, a lot of brilliant ideas and the freedom to work in my own way.\r\n\r\nProf. Dr. Reinhard Fässler for his constant support of the Sixt lab and for providing excellent working conditions. \r\n\r\nProf. Dr. Sanjiv Luther and Prof. Dr. Tobias Bollenbach for agreeing to be member of my thesis committee and to evaluate my work.\r\n\r\nDr. Walther Göhring, Carmen Schmitz, the Recombinant Protein Production core facility and the animal care takers for providing the “infrastructure” for this thesis. \r\n\r\nProf. Dr. Daniel Legler, Markus Bruckner and Dr. Julien Polleux for very fruitful collaborations and discussions.\r\n\r\nMy labmates for their help, a lot of discussions and to make the Sixt lab to a convenient place to work : Karin Hirsch, Tim Lämmeramnn, Holger Pflicke, Jörg Renkawitz, Michele Weber and Alexander Eichner All members of the Department of Molecular Medicine for their help. Especially I want to thank Sarah Schmidt, Karin Hirsch and Raphael Ruppert for their friendship, nice chats and their uncensored point of view. "},{"quality_controlled":"1","page":"714 - 724","language":[{"iso":"eng"}],"publisher":"Bentham Science Publishers","scopus_import":1,"publication":"Current Protein & Peptide Science","_id":"3287","issue":"8","author":[{"id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4088-8633","full_name":"Ruprecht, Verena","first_name":"Verena","last_name":"Ruprecht"},{"full_name":"Axmann, Markus","last_name":"Axmann","first_name":"Markus"},{"last_name":"Wieser","first_name":"Stefan","full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Schuetz, Gerhard","last_name":"Schuetz","first_name":"Gerhard"}],"date_created":"2018-12-11T12:02:28Z","department":[{"_id":"CaHe"},{"_id":"MiSi"}],"oa_version":"None","publication_status":"published","intvolume":"        12","title":"What can we learn from single molecule trajectories?","month":"12","volume":12,"status":"public","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","year":"2011","citation":{"ista":"Ruprecht V, Axmann M, Wieser S, Schuetz G. 2011. What can we learn from single molecule trajectories? Current Protein &#38; Peptide Science. 12(8), 714–724.","mla":"Ruprecht, Verena, et al. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8, Bentham Science Publishers, 2011, pp. 714–24, doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>.","short":"V. Ruprecht, M. Axmann, S. Wieser, G. Schuetz, Current Protein &#38; Peptide Science 12 (2011) 714–724.","chicago":"Ruprecht, Verena, Markus Axmann, Stefan Wieser, and Gerhard Schuetz. “What Can We Learn from Single Molecule Trajectories?” <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers, 2011. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>.","ieee":"V. Ruprecht, M. Axmann, S. Wieser, and G. Schuetz, “What can we learn from single molecule trajectories?,” <i>Current Protein &#38; Peptide Science</i>, vol. 12, no. 8. Bentham Science Publishers, pp. 714–724, 2011.","ama":"Ruprecht V, Axmann M, Wieser S, Schuetz G. What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. 2011;12(8):714-724. doi:<a href=\"https://doi.org/10.2174/138920311798841753\">10.2174/138920311798841753</a>","apa":"Ruprecht, V., Axmann, M., Wieser, S., &#38; Schuetz, G. (2011). What can we learn from single molecule trajectories? <i>Current Protein &#38; Peptide Science</i>. Bentham Science Publishers. <a href=\"https://doi.org/10.2174/138920311798841753\">https://doi.org/10.2174/138920311798841753</a>"},"date_updated":"2021-01-12T07:42:24Z","type":"journal_article","date_published":"2011-12-01T00:00:00Z","day":"01","doi":"10.2174/138920311798841753","publist_id":"3358","abstract":[{"text":"Diffusing membrane constituents are constantly exposed to a variety of forces that influence their stochastic path. Single molecule experiments allow for resolving trajectories at extremely high spatial and temporal accuracy, thereby offering insights into en route interactions of the tracer. In this review we discuss approaches to derive information about the underlying processes, based on single molecule tracking experiments. In particular, we focus on a new versatile way to analyze single molecule diffusion in the absence of a full analytical treatment. The method is based on comprehensive comparison of an experimental data set against the hypothetical outcome of multiple experiments performed on the computer. Since Monte Carlo simulations can be easily and rapidly performed even on state-of-the-art PCs, our method provides a simple way for testing various - even complicated - diffusion models. We describe the new method in detail, and show the applicability on two specific examples: firstly, kinetic rate constants can be derived for the transient interaction of mobile membrane proteins; secondly, residence time and corral size can be extracted for confined diffusion.","lang":"eng"}]},{"volume":4,"user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","status":"public","day":"08","doi":"10.1126/scisignal.2002617","publist_id":"7329","abstract":[{"text":"In their search for antigens, lymphocytes continuously shuttle among blood vessels, lymph vessels, and lymphatic tissues. Chemokines mediate entry of lymphocytes into lymphatic tissues, and sphingosine 1-phosphate (S1P) promotes localization of lymphocytes to the vasculature. Both signals are sensed through G protein-coupled receptors (GPCRs). Most GPCRs undergo ligand-dependent homologous receptor desensitization, a process that decreases their signaling output after previous exposure to high ligand concentration. Such desensitization can explain why lymphocytes do not take an intermediate position between two signals but rather oscillate between them. The desensitization of S1P receptor 1 (S1PR1) is mediated by GPCR kinase 2 (GRK2). Deletion of GRK2 in lymphocytes compromises desensitization by high vascular S1P concentrations, thereby reducing responsiveness to the chemokine signal and trapping the cells in the vascular compartment. The desensitization kinetics of S1PR1 allows lymphocytes to dynamically shuttle between vasculature and lymphatic tissue, although the positional information in both compartments is static.","lang":"eng"}],"year":"2011","citation":{"ama":"Eichner A, Sixt MK. Setting the clock for recirculating lymphocytes. <i>Science Signaling</i>. 2011;4(198). doi:<a href=\"https://doi.org/10.1126/scisignal.2002617\">10.1126/scisignal.2002617</a>","apa":"Eichner, A., &#38; Sixt, M. K. (2011). Setting the clock for recirculating lymphocytes. <i>Science Signaling</i>. American Association for the Advancement of Science. <a href=\"https://doi.org/10.1126/scisignal.2002617\">https://doi.org/10.1126/scisignal.2002617</a>","chicago":"Eichner, Alexander, and Michael K Sixt. “Setting the Clock for Recirculating Lymphocytes.” <i>Science Signaling</i>. American Association for the Advancement of Science, 2011. <a href=\"https://doi.org/10.1126/scisignal.2002617\">https://doi.org/10.1126/scisignal.2002617</a>.","ieee":"A. Eichner and M. K. Sixt, “Setting the clock for recirculating lymphocytes,” <i>Science Signaling</i>, vol. 4, no. 198. American Association for the Advancement of Science, 2011.","mla":"Eichner, Alexander, and Michael K. Sixt. “Setting the Clock for Recirculating Lymphocytes.” <i>Science Signaling</i>, vol. 4, no. 198, pe43, American Association for the Advancement of Science, 2011, doi:<a href=\"https://doi.org/10.1126/scisignal.2002617\">10.1126/scisignal.2002617</a>.","short":"A. Eichner, M.K. Sixt, Science Signaling 4 (2011).","ista":"Eichner A, Sixt MK. 2011. Setting the clock for recirculating lymphocytes. Science Signaling. 4(198), pe43."},"date_updated":"2021-01-12T08:01:02Z","type":"journal_article","date_published":"2011-11-08T00:00:00Z","publisher":"American Association for the Advancement of Science","quality_controlled":"1","language":[{"iso":"eng"}],"department":[{"_id":"MiSi"}],"date_created":"2018-12-11T11:46:46Z","publication_status":"published","oa_version":"None","intvolume":"         4","article_number":"pe43","month":"11","title":"Setting the clock for recirculating lymphocytes","scopus_import":1,"_id":"491","publication":"Science Signaling","issue":"198","author":[{"id":"4DFA52AE-F248-11E8-B48F-1D18A9856A87","last_name":"Eichner","first_name":"Alexander","full_name":"Eichner, Alexander"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}]}]