---
_id: '8924'
abstract:
- lang: eng
text: 'Maintaining fertility in a fluctuating environment is key to the reproductive
success of flowering plants. Meiosis and pollen formation are particularly sensitive
to changes in growing conditions, especially temperature. We have previously identified
cyclin-dependent kinase G1 (CDKG1) as a master regulator of temperature-dependent
meiosis and this may involve the regulation of alternative splicing (AS), including
of its own transcript. CDKG1 mRNA can undergo several AS events, potentially producing
two protein variants: CDKG1L and CDKG1S, differing in their N-terminal domain
which may be involved in co-factor interaction. In leaves, both isoforms have
distinct temperature-dependent functions on target mRNA processing, but their
role in pollen development is unknown. In the present study, we characterize the
role of CDKG1L and CDKG1S in maintaining Arabidopsis fertility. We show that the
long (L) form is necessary and sufficient to rescue the fertility defects of the
cdkg1-1 mutant, while the short (S) form is unable to rescue fertility. On the
other hand, an extra copy of CDKG1L reduces fertility. In addition, mutation of
the ATP binding pocket of the kinase indicates that kinase activity is necessary
for the function of CDKG1. Kinase mutants of CDKG1L and CDKG1S correctly localize
to the cell nucleus and nucleus and cytoplasm, respectively, but are unable to
rescue either the fertility or the splicing defects of the cdkg1-1 mutant. Furthermore,
we show that there is partial functional overlap between CDKG1 and its paralog
CDKG2 that could in part be explained by overlapping gene expression.'
acknowledgement: CN, DD, NF-F, and JD were funded by the BBSRC (grant number BB/M009459/1).
NK and AM were funded through the ERASMUS+Program. NC was funded by the VIPS Program
of the Austrian Federal Ministry of Science and Research and the City of Vienna.
article_number: '586870'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
full_name: Nibau, Candida
last_name: Nibau
- first_name: Despoina
full_name: Dadarou, Despoina
last_name: Dadarou
- first_name: Nestoras
full_name: Kargios, Nestoras
last_name: Kargios
- first_name: Areti
full_name: Mallioura, Areti
last_name: Mallioura
- first_name: Narcis
full_name: Fernandez-Fuentes, Narcis
last_name: Fernandez-Fuentes
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: John H.
full_name: Doonan, John H.
last_name: Doonan
citation:
ama: Nibau C, Dadarou D, Kargios N, et al. A functional kinase is necessary for
cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
in Arabidopsis. Frontiers in Plant Science. 2020;11. doi:10.3389/fpls.2020.586870
apa: Nibau, C., Dadarou, D., Kargios, N., Mallioura, A., Fernandez-Fuentes, N.,
Cavallari, N., & Doonan, J. H. (2020). A functional kinase is necessary for
cyclin-dependent kinase G1 (CDKG1) to maintain fertility at high ambient temperature
in Arabidopsis. Frontiers in Plant Science. Frontiers. https://doi.org/10.3389/fpls.2020.586870
chicago: Nibau, Candida, Despoina Dadarou, Nestoras Kargios, Areti Mallioura, Narcis
Fernandez-Fuentes, Nicola Cavallari, and John H. Doonan. “A Functional Kinase
Is Necessary for Cyclin-Dependent Kinase G1 (CDKG1) to Maintain Fertility at High
Ambient Temperature in Arabidopsis.” Frontiers in Plant Science. Frontiers,
2020. https://doi.org/10.3389/fpls.2020.586870.
ieee: C. Nibau et al., “A functional kinase is necessary for cyclin-dependent
kinase G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis,”
Frontiers in Plant Science, vol. 11. Frontiers, 2020.
ista: Nibau C, Dadarou D, Kargios N, Mallioura A, Fernandez-Fuentes N, Cavallari
N, Doonan JH. 2020. A functional kinase is necessary for cyclin-dependent kinase
G1 (CDKG1) to maintain fertility at high ambient temperature in Arabidopsis. Frontiers
in Plant Science. 11, 586870.
mla: Nibau, Candida, et al. “A Functional Kinase Is Necessary for Cyclin-Dependent
Kinase G1 (CDKG1) to Maintain Fertility at High Ambient Temperature in Arabidopsis.”
Frontiers in Plant Science, vol. 11, 586870, Frontiers, 2020, doi:10.3389/fpls.2020.586870.
short: C. Nibau, D. Dadarou, N. Kargios, A. Mallioura, N. Fernandez-Fuentes, N.
Cavallari, J.H. Doonan, Frontiers in Plant Science 11 (2020).
date_created: 2020-12-06T23:01:14Z
date_published: 2020-11-10T00:00:00Z
date_updated: 2023-08-24T10:50:00Z
day: '10'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2020.586870
external_id:
isi:
- '000591637000001'
file:
- access_level: open_access
checksum: 1c0ee6ce9950aa665d6a5cc64aa6b752
content_type: application/pdf
creator: dernst
date_created: 2020-12-09T09:14:19Z
date_updated: 2020-12-09T09:14:19Z
file_id: '8929'
file_name: 2020_Frontiers_Nibau.pdf
file_size: 1833244
relation: main_file
success: 1
file_date_updated: 2020-12-09T09:14:19Z
has_accepted_license: '1'
intvolume: ' 11'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_identifier:
eissn:
- 1664-462X
publication_status: published
publisher: Frontiers
quality_controlled: '1'
scopus_import: '1'
status: public
title: A functional kinase is necessary for cyclin-dependent kinase G1 (CDKG1) to
maintain fertility at high ambient temperature in Arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 11
year: '2020'
...
---
_id: '7350'
abstract:
- lang: eng
text: The ability to sense environmental temperature and to coordinate growth and
development accordingly, is critical to the reproductive success of plants. Flowering
time is regulated at the level of gene expression by a complex network of factors
that integrate environmental and developmental cues. One of the main players,
involved in modulating flowering time in response to changes in ambient temperature
is FLOWERING LOCUS M (FLM). FLM transcripts can undergo extensive alternative
splicing producing multiple variants, of which FLM-β and FLM-δ are the most representative.
While FLM-β codes for the flowering repressor FLM protein, translation of FLM-δ
has the opposite effect on flowering. Here we show that the cyclin-dependent kinase
G2 (CDKG2), together with its cognate cyclin, CYCLYN L1 (CYCL1) affects the alternative
splicing of FLM, balancing the levels of FLM-β and FLM-δ across the ambient temperature
range. In the absence of the CDKG2/CYCL1 complex, FLM-β expression is reduced
while FLM-δ is increased in a temperature dependent manner and these changes are
associated with an early flowering phenotype in the cdkg2 mutant lines. In addition,
we found that transcript variants retaining the full FLM intron 1 are sequestered
in the cell nucleus. Strikingly, FLM intron 1 splicing is also regulated by CDKG2/CYCL1.
Our results provide evidence that temperature and CDKs regulate the alternative
splicing of FLM, contributing to flowering time definition.
article_number: '1680'
article_processing_charge: No
article_type: original
author:
- first_name: Candida
full_name: Nibau, Candida
last_name: Nibau
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Despoina
full_name: Dadarou, Despoina
last_name: Dadarou
- first_name: John H.
full_name: Doonan, John H.
last_name: Doonan
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
citation:
ama: Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. Thermo-sensitive alternative
splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase G2. Frontiers
in Plant Science. 2020;10. doi:10.3389/fpls.2019.01680
apa: Nibau, C., Gallemi, M., Dadarou, D., Doonan, J. H., & Cavallari, N. (2020).
Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent
kinase G2. Frontiers in Plant Science. Frontiers Media. https://doi.org/10.3389/fpls.2019.01680
chicago: Nibau, Candida, Marçal Gallemi, Despoina Dadarou, John H. Doonan, and Nicola
Cavallari. “Thermo-Sensitive Alternative Splicing of FLOWERING LOCUS M Is Modulated
by Cyclin-Dependent Kinase G2.” Frontiers in Plant Science. Frontiers Media,
2020. https://doi.org/10.3389/fpls.2019.01680.
ieee: C. Nibau, M. Gallemi, D. Dadarou, J. H. Doonan, and N. Cavallari, “Thermo-sensitive
alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase
G2,” Frontiers in Plant Science, vol. 10. Frontiers Media, 2020.
ista: Nibau C, Gallemi M, Dadarou D, Doonan JH, Cavallari N. 2020. Thermo-sensitive
alternative splicing of FLOWERING LOCUS M is modulated by cyclin-dependent kinase
G2. Frontiers in Plant Science. 10, 1680.
mla: Nibau, Candida, et al. “Thermo-Sensitive Alternative Splicing of FLOWERING
LOCUS M Is Modulated by Cyclin-Dependent Kinase G2.” Frontiers in Plant Science,
vol. 10, 1680, Frontiers Media, 2020, doi:10.3389/fpls.2019.01680.
short: C. Nibau, M. Gallemi, D. Dadarou, J.H. Doonan, N. Cavallari, Frontiers in
Plant Science 10 (2020).
date_created: 2020-01-22T15:23:57Z
date_published: 2020-01-22T00:00:00Z
date_updated: 2023-08-17T14:21:45Z
day: '22'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3389/fpls.2019.01680
external_id:
isi:
- '000511376000001'
file:
- access_level: open_access
checksum: d1f92e60a713fbd15097ce895e5c7ccb
content_type: application/pdf
creator: dernst
date_created: 2020-01-27T09:07:02Z
date_updated: 2020-07-14T12:47:56Z
file_id: '7366'
file_name: 2020_FrontiersPlantScience_Nibau.pdf
file_size: 1951438
relation: main_file
file_date_updated: 2020-07-14T12:47:56Z
has_accepted_license: '1'
intvolume: ' 10'
isi: 1
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
publication: Frontiers in Plant Science
publication_identifier:
issn:
- 1664-462X
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Thermo-sensitive alternative splicing of FLOWERING LOCUS M is modulated by
cyclin-dependent kinase G2
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 10
year: '2020'
...
---
_id: '7427'
abstract:
- lang: eng
text: Plants, like other multicellular organisms, survive through a delicate balance
between growth and defense against pathogens. Salicylic acid (SA) is a major defense
signal in plants, and the perception mechanism as well as downstream signaling
activating the immune response are known. Here, we identify a parallel SA signaling
that mediates growth attenuation. SA directly binds to A subunits of protein phosphatase
2A (PP2A), inhibiting activity of this complex. Among PP2A targets, the PIN2 auxin
transporter is hyperphosphorylated in response to SA, leading to changed activity
of this important growth regulator. Accordingly, auxin transport and auxin-mediated
root development, including growth, gravitropic response, and lateral root organogenesis,
are inhibited. This study reveals how SA, besides activating immunity, concomitantly
attenuates growth through crosstalk with the auxin distribution network. Further
analysis of this dual role of SA and characterization of additional SA-regulated
PP2A targets will provide further insights into mechanisms maintaining a balance
between growth and defense.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: "We thank Shigeyuki Betsuyaku (University of Tsukuba), Alison Delong
(Brown University), Xinnian Dong (Duke University), Dolf Weijers (Wageningen University),
Yuelin Zhang (UBC), and Martine Pastuglia (Institut Jean-Pierre Bourgin) for sharing
published materials; Jana Riederer for help with cantharidin physiological analysis;
David Domjan for help with cloning pET28a-PIN2HL; Qing Lu for help with DARTS; Hana
Kozubı´kova´ for technical support on SA derivative synthesis; Zuzana Vondra´ kova´
for technical support with tobacco cells; Lucia Strader (Washington University),
Bert De Rybel (Ghent University), Bartel Vanholme (Ghent University), and Lukas
Mach (BOKU) for helpful discussions; and bioimaging and life science facilities
of IST Austria for continuous support. We gratefully acknowledge the Nottingham
Arabidopsis Stock Center (NASC) for providing T-DNA insertional mutants. The DSC
and SPR instruments were provided by the EQ-BOKU VIBT GmbH and the BOKU Core Facility
for Biomolecular and Cellular Analysis, with help of Irene Schaffner. The research
leading to these results has received funding from the European Union’s Horizon
2020 program (ERC grant agreement no. 742985 to J.F.) and the People Programme (Marie
Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013)
under REA grant agreement no. 291734. S.T. was supported by a European Molecular
Biology Organization (EMBO) long-term postdoctoral fellowship (ALTF 723-2015). O.N.
was supported by the Ministry of Education, Youth and Sports of the Czech Republic
(European Regional Development Fund-Project ‘‘Centre for Experimental Plant Biology’’
no. CZ.02.1.01/0.0/0.0/16_019/0000738). J. Pospısil was supported by European Regional
Development Fund Project ‘‘Centre for Experimental Plant Biology’’\r\n(no. CZ.02.1.01/0.0/0.0/16_019/0000738).
J. Petrasek was supported by EU Operational Programme Prague-Competitiveness (no.
CZ.2.16/3.1.00/21519). "
article_processing_charge: No
article_type: original
author:
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Gergely
full_name: Molnar, Gergely
id: 34F1AF46-F248-11E8-B48F-1D18A9856A87
last_name: Molnar
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Pavel
full_name: Lasák, Pavel
last_name: Lasák
- first_name: Ivan
full_name: Petřík, Ivan
last_name: Petřík
- first_name: Eugenia
full_name: Russinova, Eugenia
last_name: Russinova
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Tan S, Abas MF, Verstraeten I, et al. Salicylic acid targets protein phosphatase
2A to attenuate growth in plants. Current Biology. 2020;30(3):381-395.e8.
doi:10.1016/j.cub.2019.11.058
apa: Tan, S., Abas, M. F., Verstraeten, I., Glanc, M., Molnar, G., Hajny, J., …
Friml, J. (2020). Salicylic acid targets protein phosphatase 2A to attenuate growth
in plants. Current Biology. Cell Press. https://doi.org/10.1016/j.cub.2019.11.058
chicago: Tan, Shutang, Melinda F Abas, Inge Verstraeten, Matous Glanc, Gergely Molnar,
Jakub Hajny, Pavel Lasák, et al. “Salicylic Acid Targets Protein Phosphatase 2A
to Attenuate Growth in Plants.” Current Biology. Cell Press, 2020. https://doi.org/10.1016/j.cub.2019.11.058.
ieee: S. Tan et al., “Salicylic acid targets protein phosphatase 2A to attenuate
growth in plants,” Current Biology, vol. 30, no. 3. Cell Press, p. 381–395.e8,
2020.
ista: Tan S, Abas MF, Verstraeten I, Glanc M, Molnar G, Hajny J, Lasák P, Petřík
I, Russinova E, Petrášek J, Novák O, Pospíšil J, Friml J. 2020. Salicylic acid
targets protein phosphatase 2A to attenuate growth in plants. Current Biology.
30(3), 381–395.e8.
mla: Tan, Shutang, et al. “Salicylic Acid Targets Protein Phosphatase 2A to Attenuate
Growth in Plants.” Current Biology, vol. 30, no. 3, Cell Press, 2020, p.
381–395.e8, doi:10.1016/j.cub.2019.11.058.
short: S. Tan, M.F. Abas, I. Verstraeten, M. Glanc, G. Molnar, J. Hajny, P. Lasák,
I. Petřík, E. Russinova, J. Petrášek, O. Novák, J. Pospíšil, J. Friml, Current
Biology 30 (2020) 381–395.e8.
date_created: 2020-02-02T23:01:00Z
date_published: 2020-02-03T00:00:00Z
date_updated: 2024-03-25T23:30:20Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cub.2019.11.058
ec_funded: 1
external_id:
isi:
- '000511287900018'
pmid:
- '31956021'
file:
- access_level: open_access
checksum: 16f7d51fe28f91c21e4896a2028df40b
content_type: application/pdf
creator: dernst
date_created: 2020-09-22T09:51:28Z
date_updated: 2020-09-22T09:51:28Z
file_id: '8555'
file_name: 2020_CurrentBiology_Tan.pdf
file_size: 5360135
relation: main_file
success: 1
file_date_updated: 2020-09-22T09:51:28Z
has_accepted_license: '1'
intvolume: ' 30'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
page: 381-395.e8
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
- _id: 256FEF10-B435-11E9-9278-68D0E5697425
grant_number: 723-2015
name: Long Term Fellowship
publication: Current Biology
publication_identifier:
issn:
- '09609822'
publication_status: published
publisher: Cell Press
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Salicylic acid targets protein phosphatase 2A to attenuate growth in plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 30
year: '2020'
...
---
_id: '15037'
abstract:
- lang: eng
text: Protein abundance and localization at the plasma membrane (PM) shapes plant
development and mediates adaptation to changing environmental conditions. It is
regulated by ubiquitination, a post-translational modification crucial for the
proper sorting of endocytosed PM proteins to the vacuole for subsequent degradation.
To understand the significance and the variety of roles played by this reversible
modification, the function of ubiquitin receptors, which translate the ubiquitin
signature into a cellular response, needs to be elucidated. In this study, we
show that TOL (TOM1-like) proteins function in plants as multivalent ubiquitin
receptors, governing ubiquitinated cargo delivery to the vacuole via the conserved
Endosomal Sorting Complex Required for Transport (ESCRT) pathway. TOL2 and TOL6
interact with components of the ESCRT machinery and bind to K63-linked ubiquitin
via two tandemly arranged conserved ubiquitin-binding domains. Mutation of these
domains results not only in a loss of ubiquitin binding but also altered localization,
abolishing TOL6 ubiquitin receptor activity. Function and localization of TOL6
is itself regulated by ubiquitination, whereby TOL6 ubiquitination potentially
modulates degradation of PM-localized cargoes, assisting in the fine-tuning of
the delicate interplay between protein recycling and downregulation. Taken together,
our findings demonstrate the function and regulation of a ubiquitin receptor that
mediates vacuolar degradation of PM proteins in higher plants.
article_processing_charge: No
article_type: original
author:
- first_name: Jeanette
full_name: Moulinier-Anzola, Jeanette
last_name: Moulinier-Anzola
- first_name: Maximilian
full_name: Schwihla, Maximilian
last_name: Schwihla
- first_name: Lucinda
full_name: De-Araújo, Lucinda
last_name: De-Araújo
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Lisa
full_name: Jörg, Lisa
last_name: Jörg
- first_name: Nataliia
full_name: Konstantinova, Nataliia
last_name: Konstantinova
- first_name: Christian
full_name: Luschnig, Christian
last_name: Luschnig
- first_name: Barbara
full_name: Korbei, Barbara
last_name: Korbei
citation:
ama: Moulinier-Anzola J, Schwihla M, De-Araújo L, et al. TOLs function as ubiquitin
receptors in the early steps of the ESCRT pathway in higher plants. Molecular
Plant. 2020;13(5):717-731. doi:10.1016/j.molp.2020.02.012
apa: Moulinier-Anzola, J., Schwihla, M., De-Araújo, L., Artner, C., Jörg, L., Konstantinova,
N., … Korbei, B. (2020). TOLs function as ubiquitin receptors in the early steps
of the ESCRT pathway in higher plants. Molecular Plant. Elsevier. https://doi.org/10.1016/j.molp.2020.02.012
chicago: Moulinier-Anzola, Jeanette, Maximilian Schwihla, Lucinda De-Araújo, Christina
Artner, Lisa Jörg, Nataliia Konstantinova, Christian Luschnig, and Barbara Korbei.
“TOLs Function as Ubiquitin Receptors in the Early Steps of the ESCRT Pathway
in Higher Plants.” Molecular Plant. Elsevier, 2020. https://doi.org/10.1016/j.molp.2020.02.012.
ieee: J. Moulinier-Anzola et al., “TOLs function as ubiquitin receptors in
the early steps of the ESCRT pathway in higher plants,” Molecular Plant,
vol. 13, no. 5. Elsevier, pp. 717–731, 2020.
ista: Moulinier-Anzola J, Schwihla M, De-Araújo L, Artner C, Jörg L, Konstantinova
N, Luschnig C, Korbei B. 2020. TOLs function as ubiquitin receptors in the early
steps of the ESCRT pathway in higher plants. Molecular Plant. 13(5), 717–731.
mla: Moulinier-Anzola, Jeanette, et al. “TOLs Function as Ubiquitin Receptors in
the Early Steps of the ESCRT Pathway in Higher Plants.” Molecular Plant,
vol. 13, no. 5, Elsevier, 2020, pp. 717–31, doi:10.1016/j.molp.2020.02.012.
short: J. Moulinier-Anzola, M. Schwihla, L. De-Araújo, C. Artner, L. Jörg, N. Konstantinova,
C. Luschnig, B. Korbei, Molecular Plant 13 (2020) 717–731.
date_created: 2024-02-28T08:55:56Z
date_published: 2020-05-04T00:00:00Z
date_updated: 2024-02-28T12:41:52Z
day: '04'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1016/j.molp.2020.02.012
external_id:
pmid:
- '32087370'
file:
- access_level: open_access
checksum: c538a5008f7827f62d17d40a3bfabe65
content_type: application/pdf
creator: dernst
date_created: 2024-02-28T12:39:56Z
date_updated: 2024-02-28T12:39:56Z
file_id: '15038'
file_name: 2020_MolecularPlant_MoulinierAnzola.pdf
file_size: 3089212
relation: main_file
success: 1
file_date_updated: 2024-02-28T12:39:56Z
has_accepted_license: '1'
intvolume: ' 13'
issue: '5'
keyword:
- Plant Science
- Molecular Biology
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 717-731
pmid: 1
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: TOLs function as ubiquitin receptors in the early steps of the ESCRT pathway
in higher plants
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2020'
...
---
_id: '9160'
abstract:
- lang: eng
text: Auxin is a key hormonal regulator, that governs plant growth and development
in concert with other hormonal pathways. The unique feature of auxin is its polar,
cell-to-cell transport that leads to the formation of local auxin maxima and gradients,
which coordinate initiation and patterning of plant organs. The molecular machinery
mediating polar auxin transport is one of the important points of interaction
with other hormones. Multiple hormonal pathways converge at the regulation of
auxin transport and form a regulatory network that integrates various developmental
and environmental inputs to steer plant development. In this review, we discuss
recent advances in understanding the mechanisms that underlie regulation of polar
auxin transport by multiple hormonal pathways. Specifically, we focus on the post-translational
mechanisms that contribute to fine-tuning of the abundance and polarity of auxin
transporters at the plasma membrane and thereby enable rapid modification of the
auxin flow to coordinate plant growth and development.
acknowledgement: H.S. is the recipient of a DOC Fellowship of the Austrian Academy
of Sciences at the Institute of Science and Technology, Austria. J.C.M. is the recipient
of an EMBO Long-Term Fellowship (ALTF number 710-2016). We would like to thank Jiri
Friml and Carina Baskett for critical reading of the manuscript and Shutang Tan
and Maciek Adamowski for helpful discussions. No conflict of interest declared.
article_number: '100048'
article_processing_charge: No
article_type: original
author:
- first_name: Hana
full_name: Semeradova, Hana
id: 42FE702E-F248-11E8-B48F-1D18A9856A87
last_name: Semeradova
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: 'Semerádová H, Montesinos López JC, Benková E. All roads lead to auxin: Post-translational
regulation of auxin transport by multiple hormonal pathways. Plant Communications.
2020;1(3). doi:10.1016/j.xplc.2020.100048'
apa: 'Semerádová, H., Montesinos López, J. C., & Benková, E. (2020). All roads
lead to auxin: Post-translational regulation of auxin transport by multiple hormonal
pathways. Plant Communications. Elsevier. https://doi.org/10.1016/j.xplc.2020.100048'
chicago: 'Semerádová, Hana, Juan C Montesinos López, and Eva Benková. “All Roads
Lead to Auxin: Post-Translational Regulation of Auxin Transport by Multiple Hormonal
Pathways.” Plant Communications. Elsevier, 2020. https://doi.org/10.1016/j.xplc.2020.100048.'
ieee: 'H. Semerádová, J. C. Montesinos López, and E. Benková, “All roads lead to
auxin: Post-translational regulation of auxin transport by multiple hormonal pathways,”
Plant Communications, vol. 1, no. 3. Elsevier, 2020.'
ista: 'Semerádová H, Montesinos López JC, Benková E. 2020. All roads lead to auxin:
Post-translational regulation of auxin transport by multiple hormonal pathways.
Plant Communications. 1(3), 100048.'
mla: 'Semerádová, Hana, et al. “All Roads Lead to Auxin: Post-Translational Regulation
of Auxin Transport by Multiple Hormonal Pathways.” Plant Communications,
vol. 1, no. 3, 100048, Elsevier, 2020, doi:10.1016/j.xplc.2020.100048.'
short: H. Semerádová, J.C. Montesinos López, E. Benková, Plant Communications 1
(2020).
date_created: 2021-02-18T10:18:43Z
date_published: 2020-05-11T00:00:00Z
date_updated: 2024-03-25T23:30:26Z
day: '11'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1016/j.xplc.2020.100048
external_id:
isi:
- '000654052800010'
pmid:
- '33367243'
file:
- access_level: open_access
checksum: 785b266d82a94b007cf40dbbe7c4847e
content_type: application/pdf
creator: dernst
date_created: 2021-02-18T10:23:59Z
date_updated: 2021-02-18T10:23:59Z
file_id: '9161'
file_name: 2020_PlantComm_Semeradova.pdf
file_size: 840289
relation: main_file
success: 1
file_date_updated: 2021-02-18T10:23:59Z
has_accepted_license: '1'
intvolume: ' 1'
isi: 1
issue: '3'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 261821BC-B435-11E9-9278-68D0E5697425
grant_number: '24746'
name: Molecular mechanisms of the cytokinin regulated endomembrane trafficking to
coordinate plant organogenesis.
- _id: 253E54C8-B435-11E9-9278-68D0E5697425
grant_number: ALTF710-2016
name: Molecular mechanism of auxindriven formative divisions delineating lateral
root organogenesis in plants
publication: Plant Communications
publication_identifier:
issn:
- 2590-3462
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
record:
- id: '10135'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: 'All roads lead to auxin: Post-translational regulation of auxin transport
by multiple hormonal pathways'
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 1
year: '2020'
...
---
_id: '6897'
abstract:
- lang: eng
text: The apical hook is a transiently formed structure that plays a protective
role when the germinating seedling penetrates through the soil towards the surface.
Crucial for proper bending is the local auxin maxima, which defines the concave
(inner) side of the hook curvature. As no sign of asymmetric auxin distribution
has been reported in embryonic hypocotyls prior to hook formation, the question
of how auxin asymmetry is established in the early phases of seedling germination
remains largely unanswered. Here, we analyzed the auxin distribution and expression
of PIN auxin efflux carriers from early phases of germination, and show that bending
of the root in response to gravity is the crucial initial cue that governs the
hypocotyl bending required for apical hook formation. Importantly, polar auxin
transport machinery is established gradually after germination starts as a result
of tight root-hypocotyl interaction and a proper balance between abscisic acid
and gibberellins.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: "We thank Jiri Friml and Phillip Brewer for inspiring discussion
and for help in preparing the manuscript. This research was supported by the Scientific
Service Units (SSU) of IST-Austria through resources provided by the Bioimaging
Facility\r\n(BIF), the Life Science Facility (LSF).\r\nThis work was supported by
grants from the European Research Council (Starting Independent Research Grant ERC-2007-Stg-
207362-HCPO to E.B.). J.P. and M.S. received funds from European Regional Development
Fund-Project ‘Centre for Experimental Plant Biology’ (No. CZ.02.1.01/0.0/0.0/16_019/0000738)."
article_number: dev175919
article_processing_charge: No
article_type: original
author:
- first_name: Qiang
full_name: Zhu, Qiang
id: 40A4B9E6-F248-11E8-B48F-1D18A9856A87
last_name: Zhu
- first_name: Marçal
full_name: Gallemi, Marçal
id: 460C6802-F248-11E8-B48F-1D18A9856A87
last_name: Gallemi
orcid: 0000-0003-4675-6893
- first_name: Jiří
full_name: Pospíšil, Jiří
last_name: Pospíšil
- first_name: Petra
full_name: Žádníková, Petra
last_name: Žádníková
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. Root gravity
response module guides differential growth determining both root bending and apical
hook formation in Arabidopsis. Development. 2019;146(17). doi:10.1242/dev.175919
apa: Zhu, Q., Gallemi, M., Pospíšil, J., Žádníková, P., Strnad, M., & Benková,
E. (2019). Root gravity response module guides differential growth determining
both root bending and apical hook formation in Arabidopsis. Development.
The Company of Biologists. https://doi.org/10.1242/dev.175919
chicago: Zhu, Qiang, Marçal Gallemi, Jiří Pospíšil, Petra Žádníková, Miroslav Strnad,
and Eva Benková. “Root Gravity Response Module Guides Differential Growth Determining
Both Root Bending and Apical Hook Formation in Arabidopsis.” Development.
The Company of Biologists, 2019. https://doi.org/10.1242/dev.175919.
ieee: Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, and E. Benková,
“Root gravity response module guides differential growth determining both root
bending and apical hook formation in Arabidopsis,” Development, vol. 146,
no. 17. The Company of Biologists, 2019.
ista: Zhu Q, Gallemi M, Pospíšil J, Žádníková P, Strnad M, Benková E. 2019. Root
gravity response module guides differential growth determining both root bending
and apical hook formation in Arabidopsis. Development. 146(17), dev175919.
mla: Zhu, Qiang, et al. “Root Gravity Response Module Guides Differential Growth
Determining Both Root Bending and Apical Hook Formation in Arabidopsis.” Development,
vol. 146, no. 17, dev175919, The Company of Biologists, 2019, doi:10.1242/dev.175919.
short: Q. Zhu, M. Gallemi, J. Pospíšil, P. Žádníková, M. Strnad, E. Benková, Development
146 (2019).
date_created: 2019-09-22T22:00:36Z
date_published: 2019-09-12T00:00:00Z
date_updated: 2025-05-07T11:10:55Z
day: '12'
department:
- _id: EvBe
doi: 10.1242/dev.175919
ec_funded: 1
external_id:
isi:
- '000486297400011'
pmid:
- '31391194'
intvolume: ' 146'
isi: 1
issue: '17'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1242/dev.175919
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253FCA6A-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '207362'
name: Hormonal cross-talk in plant organogenesis
publication: Development
publication_identifier:
eissn:
- '14779129'
publication_status: published
publisher: The Company of Biologists
quality_controlled: '1'
scopus_import: '1'
status: public
title: Root gravity response module guides differential growth determining both root
bending and apical hook formation in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 146
year: '2019'
...
---
_id: '6920'
article_processing_charge: No
article_type: original
author:
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Artner C, Benková E. Ethylene and cytokinin - partners in root growth regulation.
Molecular Plant. 2019;12(10):1312-1314. doi:10.1016/j.molp.2019.09.003
apa: Artner, C., & Benková, E. (2019). Ethylene and cytokinin - partners in
root growth regulation. Molecular Plant. Cell Press. https://doi.org/10.1016/j.molp.2019.09.003
chicago: Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners
in Root Growth Regulation.” Molecular Plant. Cell Press, 2019. https://doi.org/10.1016/j.molp.2019.09.003.
ieee: C. Artner and E. Benková, “Ethylene and cytokinin - partners in root growth
regulation,” Molecular Plant, vol. 12, no. 10. Cell Press, pp. 1312–1314,
2019.
ista: Artner C, Benková E. 2019. Ethylene and cytokinin - partners in root growth
regulation. Molecular Plant. 12(10), 1312–1314.
mla: Artner, Christina, and Eva Benková. “Ethylene and Cytokinin - Partners in Root
Growth Regulation.” Molecular Plant, vol. 12, no. 10, Cell Press, 2019,
pp. 1312–14, doi:10.1016/j.molp.2019.09.003.
short: C. Artner, E. Benková, Molecular Plant 12 (2019) 1312–1314.
date_created: 2019-09-30T10:00:40Z
date_published: 2019-10-07T00:00:00Z
date_updated: 2023-08-30T06:55:02Z
day: '07'
department:
- _id: EvBe
doi: 10.1016/j.molp.2019.09.003
external_id:
isi:
- '000489132500002'
pmid:
- '31541740'
intvolume: ' 12'
isi: 1
issue: '10'
language:
- iso: eng
month: '10'
oa_version: None
page: 1312-1314
pmid: 1
project:
- _id: 2685A872-B435-11E9-9278-68D0E5697425
name: Hormonal regulation of plant adaptive responses to environmental signals
publication: Molecular Plant
publication_identifier:
issn:
- 1674-2052
- 1752-9867
publication_status: published
publisher: Cell Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ethylene and cytokinin - partners in root growth regulation
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 12
year: '2019'
...
---
_id: '7394'
article_processing_charge: No
article_type: letter_note
author:
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Yasin
full_name: Dagdas, Yasin
last_name: Dagdas
citation:
ama: 'Benková E, Dagdas Y. Editorial overview: Cell biology in the era of omics?
Current Opinion in Plant Biology. 2019;52(12):A1-A2. doi:10.1016/j.pbi.2019.11.002'
apa: 'Benková, E., & Dagdas, Y. (2019). Editorial overview: Cell biology in
the era of omics? Current Opinion in Plant Biology. Elsevier. https://doi.org/10.1016/j.pbi.2019.11.002'
chicago: 'Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the
Era of Omics?” Current Opinion in Plant Biology. Elsevier, 2019. https://doi.org/10.1016/j.pbi.2019.11.002.'
ieee: 'E. Benková and Y. Dagdas, “Editorial overview: Cell biology in the era of
omics?,” Current Opinion in Plant Biology, vol. 52, no. 12. Elsevier, pp.
A1–A2, 2019.'
ista: 'Benková E, Dagdas Y. 2019. Editorial overview: Cell biology in the era of
omics? Current Opinion in Plant Biology. 52(12), A1–A2.'
mla: 'Benková, Eva, and Yasin Dagdas. “Editorial Overview: Cell Biology in the Era
of Omics?” Current Opinion in Plant Biology, vol. 52, no. 12, Elsevier,
2019, pp. A1–2, doi:10.1016/j.pbi.2019.11.002.'
short: E. Benková, Y. Dagdas, Current Opinion in Plant Biology 52 (2019) A1–A2.
date_created: 2020-01-29T16:00:07Z
date_published: 2019-12-01T00:00:00Z
date_updated: 2023-09-07T14:56:55Z
day: '01'
department:
- _id: EvBe
doi: 10.1016/j.pbi.2019.11.002
external_id:
isi:
- '000502890600001'
pmid:
- '31787165'
intvolume: ' 52'
isi: 1
issue: '12'
language:
- iso: eng
month: '12'
oa_version: None
page: A1-A2
pmid: 1
publication: Current Opinion in Plant Biology
publication_identifier:
issn:
- 1369-5266
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Editorial overview: Cell biology in the era of omics?'
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 52
year: '2019'
...
---
_id: '6023'
abstract:
- lang: eng
text: Multicellular development requires coordinated cell polarization relative
to body axes, and translation to oriented cell division 1–3 . In plants, it is
unknown how cell polarities are connected to organismal axes and translated to
division. Here, we identify Arabidopsis SOSEKI proteins that integrate apical–basal
and radial organismal axes to localize to polar cell edges. Localization does
not depend on tissue context, requires cell wall integrity and is defined by a
transferrable, protein-specific motif. A Domain of Unknown Function in SOSEKI
proteins resembles the DIX oligomerization domain in the animal Dishevelled polarity
regulator. The DIX-like domain self-interacts and is required for edge localization
and for influencing division orientation, together with a second domain that defines
the polar membrane domain. Our work shows that SOSEKI proteins locally interpret
global polarity cues and can influence cell division orientation. Furthermore,
this work reveals that, despite fundamental differences, cell polarity mechanisms
in plants and animals converge on a similar protein domain.
article_processing_charge: No
author:
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Alja
full_name: Van Der Schuren, Alja
last_name: Van Der Schuren
- first_name: Maritza
full_name: Van Dop, Maritza
last_name: Van Dop
- first_name: Luc
full_name: Van Galen, Luc
last_name: Van Galen
- first_name: Shunsuke
full_name: Saiga, Shunsuke
last_name: Saiga
- first_name: Milad
full_name: Adibi, Milad
last_name: Adibi
- first_name: Barbara
full_name: Möller, Barbara
last_name: Möller
- first_name: Colette A.
full_name: Ten Hove, Colette A.
last_name: Ten Hove
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Richard
full_name: Smith, Richard
last_name: Smith
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
citation:
ama: Yoshida S, Van Der Schuren A, Van Dop M, et al. A SOSEKI-based coordinate system
interprets global polarity cues in arabidopsis. Nature Plants. 2019;5(2):160-166.
doi:10.1038/s41477-019-0363-6
apa: Yoshida, S., Van Der Schuren, A., Van Dop, M., Van Galen, L., Saiga, S., Adibi,
M., … Weijers, D. (2019). A SOSEKI-based coordinate system interprets global polarity
cues in arabidopsis. Nature Plants. Springer Nature. https://doi.org/10.1038/s41477-019-0363-6
chicago: Yoshida, Saiko, Alja Van Der Schuren, Maritza Van Dop, Luc Van Galen, Shunsuke
Saiga, Milad Adibi, Barbara Möller, et al. “A SOSEKI-Based Coordinate System Interprets
Global Polarity Cues in Arabidopsis.” Nature Plants. Springer Nature, 2019.
https://doi.org/10.1038/s41477-019-0363-6.
ieee: S. Yoshida et al., “A SOSEKI-based coordinate system interprets global
polarity cues in arabidopsis,” Nature Plants, vol. 5, no. 2. Springer Nature,
pp. 160–166, 2019.
ista: Yoshida S, Van Der Schuren A, Van Dop M, Van Galen L, Saiga S, Adibi M, Möller
B, Ten Hove CA, Marhavý P, Smith R, Friml J, Weijers D. 2019. A SOSEKI-based coordinate
system interprets global polarity cues in arabidopsis. Nature Plants. 5(2), 160–166.
mla: Yoshida, Saiko, et al. “A SOSEKI-Based Coordinate System Interprets Global
Polarity Cues in Arabidopsis.” Nature Plants, vol. 5, no. 2, Springer Nature,
2019, pp. 160–66, doi:10.1038/s41477-019-0363-6.
short: S. Yoshida, A. Van Der Schuren, M. Van Dop, L. Van Galen, S. Saiga, M. Adibi,
B. Möller, C.A. Ten Hove, P. Marhavý, R. Smith, J. Friml, D. Weijers, Nature Plants
5 (2019) 160–166.
date_created: 2019-02-17T22:59:21Z
date_published: 2019-02-08T00:00:00Z
date_updated: 2023-08-24T14:46:47Z
day: '08'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41477-019-0363-6
ec_funded: 1
external_id:
isi:
- '000460479600014'
intvolume: ' 5'
isi: 1
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.biorxiv.org/content/10.1101/479113v1.abstract
month: '02'
oa: 1
oa_version: Submitted Version
page: 160-166
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
publication: Nature Plants
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: A SOSEKI-based coordinate system interprets global polarity cues in arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 5
year: '2019'
...
---
_id: '6351'
abstract:
- lang: eng
text: "A process of restorative patterning in plant roots correctly replaces eliminated
cells to heal local injuries despite the absence of cell migration, which underpins
wound healing in animals. \r\n\r\nPatterning in plants relies on oriented cell
divisions and acquisition of specific cell identities. Plants regularly endure
wounds caused by abiotic or biotic environmental stimuli and have developed extraordinary
abilities to restore their tissues after injuries. Here, we provide insight into
a mechanism of restorative patterning that repairs tissues after wounding. Laser-assisted
elimination of different cells in Arabidopsis root combined with live-imaging
tracking during vertical growth allowed analysis of the regeneration processes
in vivo. Specifically, the cells adjacent to the inner side of the injury re-activated
their stem cell transcriptional programs. They accelerated their progression through
cell cycle, coordinately changed the cell division orientation, and ultimately
acquired de novo the correct cell fates to replace missing cells. These observations
highlight existence of unknown intercellular positional signaling and demonstrate
the capability of specified cells to re-acquire stem cell programs as a crucial
part of the plant-specific mechanism of wound healing."
acknowledged_ssus:
- _id: Bio
article_processing_charge: No
author:
- first_name: Petra
full_name: Marhavá, Petra
id: 44E59624-F248-11E8-B48F-1D18A9856A87
last_name: Marhavá
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
orcid: 0000-0001-8295-2926
- first_name: Saiko
full_name: Yoshida, Saiko
id: 2E46069C-F248-11E8-B48F-1D18A9856A87
last_name: Yoshida
- first_name: Peter
full_name: Marhavy, Peter
id: 3F45B078-F248-11E8-B48F-1D18A9856A87
last_name: Marhavy
orcid: 0000-0001-5227-5741
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell.
2019;177(4):957-969.e13. doi:10.1016/j.cell.2019.04.015
apa: Marhavá, P., Hörmayer, L., Yoshida, S., Marhavý, P., Benková, E., & Friml,
J. (2019). Re-activation of stem cell pathways for pattern restoration in plant
wound healing. Cell. Elsevier. https://doi.org/10.1016/j.cell.2019.04.015
chicago: Marhavá, Petra, Lukas Hörmayer, Saiko Yoshida, Peter Marhavý, Eva Benková,
and Jiří Friml. “Re-Activation of Stem Cell Pathways for Pattern Restoration in
Plant Wound Healing.” Cell. Elsevier, 2019. https://doi.org/10.1016/j.cell.2019.04.015.
ieee: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, and J. Friml,
“Re-activation of stem cell pathways for pattern restoration in plant wound healing,”
Cell, vol. 177, no. 4. Elsevier, p. 957–969.e13, 2019.
ista: Marhavá P, Hörmayer L, Yoshida S, Marhavý P, Benková E, Friml J. 2019. Re-activation
of stem cell pathways for pattern restoration in plant wound healing. Cell. 177(4),
957–969.e13.
mla: Marhavá, Petra, et al. “Re-Activation of Stem Cell Pathways for Pattern Restoration
in Plant Wound Healing.” Cell, vol. 177, no. 4, Elsevier, 2019, p. 957–969.e13,
doi:10.1016/j.cell.2019.04.015.
short: P. Marhavá, L. Hörmayer, S. Yoshida, P. Marhavý, E. Benková, J. Friml, Cell
177 (2019) 957–969.e13.
date_created: 2019-04-28T21:59:14Z
date_published: 2019-05-02T00:00:00Z
date_updated: 2024-03-25T23:30:06Z
day: '02'
ddc:
- '570'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1016/j.cell.2019.04.015
ec_funded: 1
external_id:
isi:
- '000466843000015'
pmid:
- '31051107'
file:
- access_level: open_access
checksum: 4ceba04a96a74f5092ec3ce2c579a0c7
content_type: application/pdf
creator: dernst
date_created: 2019-05-13T06:12:45Z
date_updated: 2020-07-14T12:47:28Z
file_id: '6411'
file_name: 2019_Cell_Marhava.pdf
file_size: 10272032
relation: main_file
file_date_updated: 2020-07-14T12:47:28Z
has_accepted_license: '1'
intvolume: ' 177'
isi: 1
issue: '4'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
page: 957-969.e13
pmid: 1
project:
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Cell
publication_identifier:
eissn:
- '10974172'
issn:
- '00928674'
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/specialized-plant-cells-regain-stem-cell-features-to-heal-wounds/
record:
- id: '9992'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Re-activation of stem cell pathways for pattern restoration in plant wound
healing
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 177
year: '2019'
...
---
_id: '277'
abstract:
- lang: eng
text: 'Arabidopsis and human ARM protein interact with telomerase. Deregulated mRNA
levels of DNA repair and ribosomal protein genes in an Arabidopsis arm mutant
suggest non-telomeric ARM function. The human homolog ARMC6 interacts with hTRF2.
Abstract: Telomerase maintains telomeres and has proposed non-telomeric functions.
We previously identified interaction of the C-terminal domain of Arabidopsis telomerase
reverse transcriptase (AtTERT) with an armadillo/β-catenin-like repeat (ARM) containing
protein. Here we explore protein–protein interactions of the ARM protein, AtTERT
domains, POT1a, TRF-like family and SMH family proteins, and the chromatin remodeling
protein CHR19 using bimolecular fluorescence complementation (BiFC), yeast two-hybrid
(Y2H) analysis, and co-immunoprecipitation. The ARM protein interacts with both
the N- and C-terminal domains of AtTERT in different cellular compartments. ARM
interacts with CHR19 and TRF-like I family proteins that also bind AtTERT directly
or through interaction with POT1a. The putative human ARM homolog co-precipitates
telomerase activity and interacts with hTRF2 protein in vitro. Analysis of Arabidopsis
arm mutants shows no obvious changes in telomere length or telomerase activity,
suggesting that ARM is not essential for telomere maintenance. The observed interactions
with telomerase and Myb-like domain proteins (TRF-like family I) may therefore
reflect possible non-telomeric functions. Transcript levels of several DNA repair
and ribosomal genes are affected in arm mutants, and ARM, likely in association
with other proteins, suppressed expression of XRCC3 and RPSAA promoter constructs
in luciferase reporter assays. In conclusion, ARM can participate in non-telomeric
functions of telomerase, and can also perform its own telomerase-independent functions.'
article_processing_charge: No
article_type: original
author:
- first_name: Ladislav
full_name: Dokládal, Ladislav
last_name: Dokládal
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: David
full_name: Honys, David
last_name: Honys
- first_name: Nikoleta
full_name: Dupláková, Nikoleta
last_name: Dupláková
- first_name: Lan
full_name: Lee, Lan
last_name: Lee
- first_name: Stanton
full_name: Gelvin, Stanton
last_name: Gelvin
- first_name: Eva
full_name: Sýkorová, Eva
last_name: Sýkorová
citation:
ama: Dokládal L, Benková E, Honys D, et al. An armadillo-domain protein participates
in a telomerase interaction network. Plant Molecular Biology. 2018;97(5):407-420.
doi:10.1007/s11103-018-0747-4
apa: Dokládal, L., Benková, E., Honys, D., Dupláková, N., Lee, L., Gelvin, S., &
Sýkorová, E. (2018). An armadillo-domain protein participates in a telomerase
interaction network. Plant Molecular Biology. Springer. https://doi.org/10.1007/s11103-018-0747-4
chicago: Dokládal, Ladislav, Eva Benková, David Honys, Nikoleta Dupláková, Lan Lee,
Stanton Gelvin, and Eva Sýkorová. “An Armadillo-Domain Protein Participates in
a Telomerase Interaction Network.” Plant Molecular Biology. Springer, 2018.
https://doi.org/10.1007/s11103-018-0747-4.
ieee: L. Dokládal et al., “An armadillo-domain protein participates in a
telomerase interaction network,” Plant Molecular Biology, vol. 97, no.
5. Springer, pp. 407–420, 2018.
ista: Dokládal L, Benková E, Honys D, Dupláková N, Lee L, Gelvin S, Sýkorová E.
2018. An armadillo-domain protein participates in a telomerase interaction network.
Plant Molecular Biology. 97(5), 407–420.
mla: Dokládal, Ladislav, et al. “An Armadillo-Domain Protein Participates in a Telomerase
Interaction Network.” Plant Molecular Biology, vol. 97, no. 5, Springer,
2018, pp. 407–20, doi:10.1007/s11103-018-0747-4.
short: L. Dokládal, E. Benková, D. Honys, N. Dupláková, L. Lee, S. Gelvin, E. Sýkorová,
Plant Molecular Biology 97 (2018) 407–420.
date_created: 2018-12-11T11:45:34Z
date_published: 2018-06-12T00:00:00Z
date_updated: 2023-09-08T13:21:05Z
day: '12'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1007/s11103-018-0747-4
external_id:
isi:
- '000438981700009'
file:
- access_level: open_access
checksum: 451ae47616e6af2533099f596b2a47fb
content_type: application/pdf
creator: dernst
date_created: 2020-05-14T12:23:08Z
date_updated: 2020-07-14T12:45:45Z
file_id: '7834'
file_name: 2018_PlantMolecBio_Dokladal.pdf
file_size: 1150679
relation: main_file
file_date_updated: 2020-07-14T12:45:45Z
has_accepted_license: '1'
intvolume: ' 97'
isi: 1
issue: '5'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Submitted Version
page: 407 - 420
publication: Plant Molecular Biology
publication_status: published
publisher: Springer
publist_id: '7625'
quality_controlled: '1'
scopus_import: '1'
status: public
title: An armadillo-domain protein participates in a telomerase interaction network
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 97
year: '2018'
...
---
_id: '283'
abstract:
- lang: eng
text: Light represents the principal signal driving circadian clock entrainment.
However, how light influences the evolution of the clock remains poorly understood.
The cavefish Phreatichthys andruzzii represents a fascinating model to explore
how evolution under extreme aphotic conditions shapes the circadian clock, since
in this species the clock is unresponsive to light. We have previously demonstrated
that loss-of-function mutations targeting non-visual opsins contribute in part
to this blind clock phenotype. Here, we have compared orthologs of two core clock
genes that play a key role in photic entrainment, cry1a and per2, in both zebrafish
and P. andruzzii. We encountered aberrantly spliced variants for the P. andruzzii
per2 transcript. The most abundant transcript encodes a truncated protein lacking
the C-terminal Cry binding domain and incorporating an intronic, transposon-derived
coding sequence. We demonstrate that the transposon insertion leads to a predominantly
cytoplasmic localization of the cavefish Per2 protein in contrast to the zebrafish
ortholog which is distributed in both the nucleus and cytoplasm. Thus, it seems
that during evolution in complete darkness, the photic entrainment pathway of
the circadian clock has been subject to mutation at multiple levels, extending
from opsin photoreceptors to nuclear effectors.
article_number: '8754'
article_processing_charge: No
author:
- first_name: Rosa Maria
full_name: Ceinos, Rosa Maria
last_name: Ceinos
- first_name: Elena
full_name: Frigato, Elena
last_name: Frigato
- first_name: Cristina
full_name: Pagano, Cristina
last_name: Pagano
- first_name: Nadine
full_name: Frohlich, Nadine
last_name: Frohlich
- first_name: Pietro
full_name: Negrini, Pietro
last_name: Negrini
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: Daniela
full_name: Vallone, Daniela
last_name: Vallone
- first_name: Silvia
full_name: Fuselli, Silvia
last_name: Fuselli
- first_name: Cristiano
full_name: Bertolucci, Cristiano
last_name: Bertolucci
- first_name: Nicholas S
full_name: Foulkes, Nicholas S
last_name: Foulkes
citation:
ama: Ceinos RM, Frigato E, Pagano C, et al. Mutations in blind cavefish target the
light regulated circadian clock gene period 2. Scientific Reports. 2018;8(1).
doi:10.1038/s41598-018-27080-2
apa: Ceinos, R. M., Frigato, E., Pagano, C., Frohlich, N., Negrini, P., Cavallari,
N., … Foulkes, N. S. (2018). Mutations in blind cavefish target the light regulated
circadian clock gene period 2. Scientific Reports. Nature Publishing Group.
https://doi.org/10.1038/s41598-018-27080-2
chicago: Ceinos, Rosa Maria, Elena Frigato, Cristina Pagano, Nadine Frohlich, Pietro
Negrini, Nicola Cavallari, Daniela Vallone, Silvia Fuselli, Cristiano Bertolucci,
and Nicholas S Foulkes. “Mutations in Blind Cavefish Target the Light Regulated
Circadian Clock Gene Period 2.” Scientific Reports. Nature Publishing Group,
2018. https://doi.org/10.1038/s41598-018-27080-2.
ieee: R. M. Ceinos et al., “Mutations in blind cavefish target the light
regulated circadian clock gene period 2,” Scientific Reports, vol. 8, no.
1. Nature Publishing Group, 2018.
ista: Ceinos RM, Frigato E, Pagano C, Frohlich N, Negrini P, Cavallari N, Vallone
D, Fuselli S, Bertolucci C, Foulkes NS. 2018. Mutations in blind cavefish target
the light regulated circadian clock gene period 2. Scientific Reports. 8(1), 8754.
mla: Ceinos, Rosa Maria, et al. “Mutations in Blind Cavefish Target the Light Regulated
Circadian Clock Gene Period 2.” Scientific Reports, vol. 8, no. 1, 8754,
Nature Publishing Group, 2018, doi:10.1038/s41598-018-27080-2.
short: R.M. Ceinos, E. Frigato, C. Pagano, N. Frohlich, P. Negrini, N. Cavallari,
D. Vallone, S. Fuselli, C. Bertolucci, N.S. Foulkes, Scientific Reports 8 (2018).
date_created: 2018-12-11T11:45:36Z
date_published: 2018-06-08T00:00:00Z
date_updated: 2023-09-13T08:59:27Z
day: '08'
ddc:
- '570'
department:
- _id: EvBe
doi: 10.1038/s41598-018-27080-2
external_id:
isi:
- '000434640800008'
file:
- access_level: open_access
checksum: 9c3942d772f84f3df032ffde0ed9a8ea
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T13:04:46Z
date_updated: 2020-07-14T12:45:49Z
file_id: '5707'
file_name: 2018_ScientificReports_Ceinos.pdf
file_size: 1855324
relation: main_file
file_date_updated: 2020-07-14T12:45:49Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
publist_id: '7616'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Mutations in blind cavefish target the light regulated circadian clock gene
period 2
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '191'
abstract:
- lang: eng
text: Intercellular distribution of the plant hormone auxin largely depends on the
polar subcellular distribution of the plasma membrane PIN-FORMED (PIN) auxin transporters.
PIN polarity switches in response to different developmental and environmental
signals have been shown to redirect auxin fluxes mediating certain developmental
responses. PIN phosphorylation at different sites and by different kinases is
crucial for PIN function. Here we investigate the role of PIN phosphorylation
during gravitropic response. Loss- and gain-of-function mutants in PINOID and
related kinases but not in D6PK kinase as well as mutations mimicking constitutive
dephosphorylated or phosphorylated status of two clusters of predicted phosphorylation
sites partially disrupted PIN3 phosphorylation and caused defects in gravitropic
bending in roots and hypocotyls. In particular, they impacted PIN3 polarity rearrangements
in response to gravity and during feed-back regulation by auxin itself. Thus PIN
phosphorylation, besides regulating transport activity and apical-basal targeting,
is also important for the rapid polarity switches in response to environmental
and endogenous signals.
article_number: '10279'
article_processing_charge: No
author:
- first_name: Peter
full_name: Grones, Peter
id: 399876EC-F248-11E8-B48F-1D18A9856A87
last_name: Grones
- first_name: Melinda F
full_name: Abas, Melinda F
id: 3CFB3B1C-F248-11E8-B48F-1D18A9856A87
last_name: Abas
- first_name: Jakub
full_name: Hajny, Jakub
id: 4800CC20-F248-11E8-B48F-1D18A9856A87
last_name: Hajny
orcid: 0000-0003-2140-7195
- first_name: Angharad
full_name: Jones, Angharad
last_name: Jones
- first_name: Sascha
full_name: Waidmann, Sascha
last_name: Waidmann
- first_name: Jürgen
full_name: Kleine Vehn, Jürgen
last_name: Kleine Vehn
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Grones P, Abas MF, Hajny J, et al. PID/WAG-mediated phosphorylation of the
Arabidopsis PIN3 auxin transporter mediates polarity switches during gravitropism.
Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-28188-1
apa: Grones, P., Abas, M. F., Hajny, J., Jones, A., Waidmann, S., Kleine Vehn, J.,
& Friml, J. (2018). PID/WAG-mediated phosphorylation of the Arabidopsis PIN3
auxin transporter mediates polarity switches during gravitropism. Scientific
Reports. Springer. https://doi.org/10.1038/s41598-018-28188-1
chicago: Grones, Peter, Melinda F Abas, Jakub Hajny, Angharad Jones, Sascha Waidmann,
Jürgen Kleine Vehn, and Jiří Friml. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports. Springer, 2018. https://doi.org/10.1038/s41598-018-28188-1.
ieee: P. Grones et al., “PID/WAG-mediated phosphorylation of the Arabidopsis
PIN3 auxin transporter mediates polarity switches during gravitropism,” Scientific
Reports, vol. 8, no. 1. Springer, 2018.
ista: Grones P, Abas MF, Hajny J, Jones A, Waidmann S, Kleine Vehn J, Friml J. 2018.
PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter mediates
polarity switches during gravitropism. Scientific Reports. 8(1), 10279.
mla: Grones, Peter, et al. “PID/WAG-Mediated Phosphorylation of the Arabidopsis
PIN3 Auxin Transporter Mediates Polarity Switches during Gravitropism.” Scientific
Reports, vol. 8, no. 1, 10279, Springer, 2018, doi:10.1038/s41598-018-28188-1.
short: P. Grones, M.F. Abas, J. Hajny, A. Jones, S. Waidmann, J. Kleine Vehn, J.
Friml, Scientific Reports 8 (2018).
date_created: 2018-12-11T11:45:06Z
date_published: 2018-07-06T00:00:00Z
date_updated: 2025-05-07T11:12:31Z
day: '06'
ddc:
- '581'
department:
- _id: JiFr
- _id: EvBe
doi: 10.1038/s41598-018-28188-1
ec_funded: 1
external_id:
isi:
- '000437673200053'
file:
- access_level: open_access
checksum: 266b03f4fb8198e83141617aaa99dcab
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T15:38:56Z
date_updated: 2020-07-14T12:45:20Z
file_id: '5714'
file_name: 2018_ScientificReports_Grones.pdf
file_size: 2413876
relation: main_file
file_date_updated: 2020-07-14T12:45:20Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
- _id: 261099A6-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742985'
name: Tracing Evolution of Auxin Transport and Polarity in Plants
publication: Scientific Reports
publication_status: published
publisher: Springer
publist_id: '7729'
quality_controlled: '1'
related_material:
record:
- id: '8822'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: PID/WAG-mediated phosphorylation of the Arabidopsis PIN3 auxin transporter
mediates polarity switches during gravitropism
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...
---
_id: '47'
abstract:
- lang: eng
text: Plant hormones as signalling molecules play an essential role in the control
of plant growth and development. Typically, sites of hormonal action are usually
distant from the site of biosynthesis thus relying on efficient transport mechanisms.
Over the last decades, molecular identification of proteins and protein complexes
involved in hormonal transport has started. Advanced screens for genes involved
in hormonal transport in combination with transport assays using heterologous
systems such as yeast, insect, or tobacco BY2 cells or Xenopus oocytes provided
important insights into mechanisms underlying distribution of hormones in plant
body and led to identification of principal transporters for each hormone. This
review gives a short overview of the mechanisms of hormonal transport and transporters
identified in Arabidopsis thaliana.
article_processing_charge: No
author:
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Benoît
full_name: Lacombe, Benoît
last_name: Lacombe
citation:
ama: Abualia R, Benková E, Lacombe B. Transporters and mechanisms of hormone transport
in arabidopsis. Advances in Botanical Research. 2018;87:115-138. doi:10.1016/bs.abr.2018.09.007
apa: Abualia, R., Benková, E., & Lacombe, B. (2018). Transporters and mechanisms
of hormone transport in arabidopsis. Advances in Botanical Research. Elsevier.
https://doi.org/10.1016/bs.abr.2018.09.007
chicago: Abualia, Rashed, Eva Benková, and Benoît Lacombe. “Transporters and Mechanisms
of Hormone Transport in Arabidopsis.” Advances in Botanical Research. Elsevier,
2018. https://doi.org/10.1016/bs.abr.2018.09.007.
ieee: R. Abualia, E. Benková, and B. Lacombe, “Transporters and mechanisms of hormone
transport in arabidopsis,” Advances in Botanical Research, vol. 87. Elsevier,
pp. 115–138, 2018.
ista: Abualia R, Benková E, Lacombe B. 2018. Transporters and mechanisms of hormone
transport in arabidopsis. Advances in Botanical Research. 87, 115–138.
mla: Abualia, Rashed, et al. “Transporters and Mechanisms of Hormone Transport in
Arabidopsis.” Advances in Botanical Research, vol. 87, Elsevier, 2018,
pp. 115–38, doi:10.1016/bs.abr.2018.09.007.
short: R. Abualia, E. Benková, B. Lacombe, Advances in Botanical Research 87 (2018)
115–138.
date_created: 2018-12-11T11:44:20Z
date_published: 2018-01-01T00:00:00Z
date_updated: 2024-03-25T23:30:22Z
day: '01'
department:
- _id: EvBe
doi: 10.1016/bs.abr.2018.09.007
external_id:
isi:
- '000453657800006'
intvolume: ' 87'
isi: 1
language:
- iso: eng
month: '01'
oa_version: None
page: 115 - 138
publication: Advances in Botanical Research
publication_status: published
publisher: Elsevier
publist_id: '8007'
quality_controlled: '1'
related_material:
record:
- id: '10303'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Transporters and mechanisms of hormone transport in arabidopsis
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 87
year: '2018'
...
---
_id: '539'
abstract:
- lang: eng
text: The whole life cycle of plants as well as their responses to environmental
stimuli is governed by a complex network of hormonal regulations. A number of
studies have demonstrated an essential role of both auxin and cytokinin in the
regulation of many aspects of plant growth and development including embryogenesis,
postembryonic organogenic processes such as root, and shoot branching, root and
shoot apical meristem activity and phyllotaxis. Over the last decades essential
knowledge on the key molecular factors and pathways that spatio-temporally define
auxin and cytokinin activities in the plant body has accumulated. However, how
both hormonal pathways are interconnected by a complex network of interactions
and feedback circuits that determines the final outcome of the individual hormone
actions is still largely unknown. Root system architecture establishment and in
particular formation of lateral organs is prime example of developmental process
at whose regulation both auxin and cytokinin pathways converge. To dissect convergence
points and pathways that tightly balance auxin - cytokinin antagonistic activities
that determine the root branching pattern transcriptome profiling was applied.
Genome wide expression analyses of the xylem pole pericycle, a tissue giving rise
to lateral roots, led to identification of genes that are highly responsive to
combinatorial auxin and cytokinin treatments and play an essential function in
the auxin-cytokinin regulated root branching. SYNERGISTIC AUXIN CYTOKININ 1 (SYAC1)
gene, which encodes for a protein of unknown function, was detected among the
top candidate genes of which expression was synergistically up-regulated by simultaneous
hormonal treatment. Plants with modulated SYAC1 activity exhibit severe defects
in the root system establishment and attenuate developmental responses to both
auxin and cytokinin. To explore the biological function of the SYAC1, we employed
different strategies including expression pattern analysis, subcellular localization
and phenotypic analyses of the syac1 loss-of-function and gain-of-function transgenic
lines along with the identification of the SYAC1 interaction partners. Detailed
functional characterization revealed that SYAC1 acts as a developmentally specific
regulator of the secretory pathway to control deposition of cell wall components
and thereby rapidly fine tune elongation growth.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Andrej
full_name: Hurny, Andrej
id: 4DC4AF46-F248-11E8-B48F-1D18A9856A87
last_name: Hurny
orcid: 0000-0003-3638-1426
citation:
ama: Hurny A. Identification and characterization of novel auxin-cytokinin cross-talk
components. 2018. doi:10.15479/AT:ISTA:th_930
apa: Hurny, A. (2018). Identification and characterization of novel auxin-cytokinin
cross-talk components. Institute of Science and Technology Austria. https://doi.org/10.15479/AT:ISTA:th_930
chicago: Hurny, Andrej. “Identification and Characterization of Novel Auxin-Cytokinin
Cross-Talk Components.” Institute of Science and Technology Austria, 2018. https://doi.org/10.15479/AT:ISTA:th_930.
ieee: A. Hurny, “Identification and characterization of novel auxin-cytokinin cross-talk
components,” Institute of Science and Technology Austria, 2018.
ista: Hurny A. 2018. Identification and characterization of novel auxin-cytokinin
cross-talk components. Institute of Science and Technology Austria.
mla: Hurny, Andrej. Identification and Characterization of Novel Auxin-Cytokinin
Cross-Talk Components. Institute of Science and Technology Austria, 2018,
doi:10.15479/AT:ISTA:th_930.
short: A. Hurny, Identification and Characterization of Novel Auxin-Cytokinin Cross-Talk
Components, Institute of Science and Technology Austria, 2018.
date_created: 2018-12-11T11:47:03Z
date_published: 2018-01-01T00:00:00Z
date_updated: 2023-09-07T12:41:06Z
day: '01'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: EvBe
doi: 10.15479/AT:ISTA:th_930
file:
- access_level: closed
checksum: 0c9d6d1c80d9857e6e545213467bbcb2
content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
creator: dernst
date_created: 2019-04-05T09:37:56Z
date_updated: 2020-12-02T23:30:08Z
embargo_to: open_access
file_id: '6226'
file_name: 2018_Hurny_thesis_source.docx
file_size: 28112114
relation: source_file
- access_level: open_access
checksum: ecbe481a1413d270bd501b872c7ed54f
content_type: application/pdf
creator: dernst
date_created: 2019-04-05T09:37:55Z
date_updated: 2020-12-02T09:52:16Z
embargo: 2019-07-10
file_id: '6227'
file_name: 2018_Hurny_thesis.pdf
file_size: 12524427
relation: main_file
file_date_updated: 2020-12-02T23:30:08Z
has_accepted_license: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
page: '147'
publication_identifier:
issn:
- 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '7277'
pubrep_id: '930'
related_material:
record:
- id: '1024'
relation: part_of_dissertation
status: public
status: public
supervisor:
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
title: Identification and characterization of novel auxin-cytokinin cross-talk components
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: dissertation
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
year: '2018'
...
---
_id: '403'
abstract:
- lang: eng
text: The ability to adapt growth and development to temperature variations is crucial
to generate plant varieties resilient to predicted temperature changes. However,
the mechanisms underlying plant response to progressive increases in temperature
have just started to be elucidated. Here, we report that the Cyclin-dependent
Kinase G1 (CDKG1) is a central element in a thermo-sensitive mRNA splicing cascade
that transduces changes in ambient temperature into differential expression of
the fundamental spliceosome component, ATU2AF65A. CDKG1 is alternatively spliced
in a temperature-dependent manner. We found that this process is partly dependent
on both the Cyclin-dependent Kinase G2 (CDKG2) and the interacting co-factor CYCLIN
L1 resulting in two distinct messenger RNAs. Relative abundance of both CDKG1
transcripts correlates with ambient temperature and possibly with different expression
levels of the associated protein isoforms. Both CDKG1 alternative transcripts
are necessary to fully complement the expression of ATU2AF65A across the temperature
range. Our data support a previously unidentified temperature-dependent mechanism
based on the alternative splicing of CDKG1 and regulated by CDKG2 and CYCLIN L1.
We propose that changes in ambient temperature affect the relative abundance of
CDKG1 transcripts and this in turn translates into differential CDKG1 protein
expression coordinating the alternative splicing of ATU2AF65A. This article is
protected by copyright. All rights reserved.
acknowledgement: CN, DD and JHD were funded by the BBSRC (grant number BB/M009459/1).
NC was funded by the VIPS Program of the Austrian Federal Ministry of Science and
Research and the City of Vienna. AB and AF were supported by the Austrian Science
Fund (FWF) [DK W1207; SFB RNAreg F43-P10]
article_processing_charge: No
author:
- first_name: Nicola
full_name: Cavallari, Nicola
id: 457160E6-F248-11E8-B48F-1D18A9856A87
last_name: Cavallari
- first_name: Candida
full_name: Nibau, Candida
last_name: Nibau
- first_name: Armin
full_name: Fuchs, Armin
last_name: Fuchs
- first_name: Despoina
full_name: Dadarou, Despoina
last_name: Dadarou
- first_name: Andrea
full_name: Barta, Andrea
last_name: Barta
- first_name: John
full_name: Doonan, John
last_name: Doonan
citation:
ama: Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. The cyclin‐dependent
kinase G group defines a thermo‐sensitive alternative splicing circuit modulating
the expression of Arabidopsis ATU 2AF 65A. The Plant Journal. 2018;94(6):1010-1022.
doi:10.1111/tpj.13914
apa: Cavallari, N., Nibau, C., Fuchs, A., Dadarou, D., Barta, A., & Doonan,
J. (2018). The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative
splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A. The
Plant Journal. Wiley. https://doi.org/10.1111/tpj.13914
chicago: Cavallari, Nicola, Candida Nibau, Armin Fuchs, Despoina Dadarou, Andrea
Barta, and John Doonan. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive
Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF
65A.” The Plant Journal. Wiley, 2018. https://doi.org/10.1111/tpj.13914.
ieee: N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, and J. Doonan, “The
cyclin‐dependent kinase G group defines a thermo‐sensitive alternative splicing
circuit modulating the expression of Arabidopsis ATU 2AF 65A,” The Plant Journal,
vol. 94, no. 6. Wiley, pp. 1010–1022, 2018.
ista: Cavallari N, Nibau C, Fuchs A, Dadarou D, Barta A, Doonan J. 2018. The cyclin‐dependent
kinase G group defines a thermo‐sensitive alternative splicing circuit modulating
the expression of Arabidopsis ATU 2AF 65A. The Plant Journal. 94(6), 1010–1022.
mla: Cavallari, Nicola, et al. “The Cyclin‐dependent Kinase G Group Defines a Thermo‐sensitive
Alternative Splicing Circuit Modulating the Expression of Arabidopsis ATU 2AF
65A.” The Plant Journal, vol. 94, no. 6, Wiley, 2018, pp. 1010–22, doi:10.1111/tpj.13914.
short: N. Cavallari, C. Nibau, A. Fuchs, D. Dadarou, A. Barta, J. Doonan, The Plant
Journal 94 (2018) 1010–1022.
date_created: 2018-12-11T11:46:17Z
date_published: 2018-06-01T00:00:00Z
date_updated: 2023-09-19T10:07:08Z
day: '01'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.1111/tpj.13914
external_id:
isi:
- '000434365500008'
file:
- access_level: open_access
checksum: d9d3ad3215ac0e581731443fca312266
content_type: application/pdf
creator: dernst
date_created: 2019-02-06T11:40:54Z
date_updated: 2020-07-14T12:46:22Z
file_id: '5934'
file_name: 2018_PlantJourn_Cavallari.pdf
file_size: 1543354
relation: main_file
file_date_updated: 2020-07-14T12:46:22Z
has_accepted_license: '1'
intvolume: ' 94'
isi: 1
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 1010 - 1022
publication: The Plant Journal
publication_status: published
publisher: Wiley
publist_id: '7426'
quality_controlled: '1'
scopus_import: '1'
status: public
title: The cyclin‐dependent kinase G group defines a thermo‐sensitive alternative
splicing circuit modulating the expression of Arabidopsis ATU 2AF 65A
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 94
year: '2018'
...
---
_id: '407'
abstract:
- lang: eng
text: Isoprenoid cytokinins play a number of crucial roles in the regulation of
plant growth and development. To study cytokinin receptor properties in plants,
we designed and prepared fluorescent derivatives of 6-[(3-methylbut-2-en-1-yl)amino]purine
(N6-isopentenyladenine, iP) with several fluorescent labels attached to the C2
or N9 atom of the purine moiety via a 2- or 6-carbon linker. The fluorescent labels
included dansyl (DS), fluorescein (FC), 7-nitrobenzofurazan (NBD), rhodamine B
(RhoB), coumarin (Cou), 7-(diethylamino)coumarin (DEAC) and cyanine 5 dye (Cy5).
All prepared compounds were screened for affinity for the Arabidopsis thaliana
cytokinin receptor (CRE1/AHK4). Although the attachment of the fluorescent labels
to iP via the linkers mostly disrupted binding to the receptor, several fluorescent
derivatives interacted well. For this reason, three derivatives, two rhodamine
B and one 4-chloro-7-nitrobenzofurazan labeled iP were tested for their interaction
with CRE1/AHK4 and Zea mays cytokinin receptors in detail. We further showed that
the three derivatives were able to activate transcription of cytokinin response
regulator ARR5 in Arabidopsis seedlings. The activity of fluorescently labeled
cytokinins was compared with corresponding 6-dimethylaminopurine fluorescently
labeled negative controls. Selected rhodamine B C2-labeled compounds 17, 18 and
4-chloro-7-nitrobenzofurazan N9-labeled compound 28 and their respective negative
controls (19, 20 and 29, respectively) were used for in planta staining experiments
in Arabidopsis thaliana cell suspension culture using live cell confocal microscopy.
acknowledgement: "This work was supported by the Ministry of Education Youth and Sports,
Czech Republic (grant LO1204 from the National Program of Sustainability I and Agricultural
Research ) and by Czech Science Foundation grants 16-04184S , 501/10/1450 and 13-39982S
and by IGA projects IGA_PrF_2018_033 and IGA_PrF_2018_023 . We would like to thank
Jarmila Balonová, Olga Hustáková and Miroslava Šubová for their skillful technical
assistance and Mgr. Tomáš Pospíšil, Ph.D. for his measurement of 1 H NMR and analysis
of some 2D NMR spectral data. \r\n"
article_processing_charge: No
author:
- first_name: Karolina
full_name: Kubiasová, Karolina
last_name: Kubiasová
- first_name: Václav
full_name: Mik, Václav
last_name: Mik
- first_name: Jaroslav
full_name: Nisler, Jaroslav
last_name: Nisler
- first_name: Martin
full_name: Hönig, Martin
last_name: Hönig
- first_name: Alexandra
full_name: Husičková, Alexandra
last_name: Husičková
- first_name: Lukáš
full_name: Spíchal, Lukáš
last_name: Spíchal
- first_name: Zuzana
full_name: Pěkná, Zuzana
last_name: Pěkná
- first_name: Olga
full_name: Šamajová, Olga
last_name: Šamajová
- first_name: Karel
full_name: Doležal, Karel
last_name: Doležal
- first_name: Ondřej
full_name: Plíhal, Ondřej
last_name: Plíhal
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Miroslav
full_name: Strnad, Miroslav
last_name: Strnad
- first_name: Lucie
full_name: Plíhalová, Lucie
last_name: Plíhalová
citation:
ama: Kubiasová K, Mik V, Nisler J, et al. Design, synthesis and perception of fluorescently
labeled isoprenoid cytokinins. Phytochemistry. 2018;150:1-11. doi:10.1016/j.phytochem.2018.02.015
apa: Kubiasová, K., Mik, V., Nisler, J., Hönig, M., Husičková, A., Spíchal, L.,
… Plíhalová, L. (2018). Design, synthesis and perception of fluorescently labeled
isoprenoid cytokinins. Phytochemistry. Elsevier. https://doi.org/10.1016/j.phytochem.2018.02.015
chicago: Kubiasová, Karolina, Václav Mik, Jaroslav Nisler, Martin Hönig, Alexandra
Husičková, Lukáš Spíchal, Zuzana Pěkná, et al. “Design, Synthesis and Perception
of Fluorescently Labeled Isoprenoid Cytokinins.” Phytochemistry. Elsevier,
2018. https://doi.org/10.1016/j.phytochem.2018.02.015.
ieee: K. Kubiasová et al., “Design, synthesis and perception of fluorescently
labeled isoprenoid cytokinins,” Phytochemistry, vol. 150. Elsevier, pp.
1–11, 2018.
ista: Kubiasová K, Mik V, Nisler J, Hönig M, Husičková A, Spíchal L, Pěkná Z, Šamajová
O, Doležal K, Plíhal O, Benková E, Strnad M, Plíhalová L. 2018. Design, synthesis
and perception of fluorescently labeled isoprenoid cytokinins. Phytochemistry.
150, 1–11.
mla: Kubiasová, Karolina, et al. “Design, Synthesis and Perception of Fluorescently
Labeled Isoprenoid Cytokinins.” Phytochemistry, vol. 150, Elsevier, 2018,
pp. 1–11, doi:10.1016/j.phytochem.2018.02.015.
short: K. Kubiasová, V. Mik, J. Nisler, M. Hönig, A. Husičková, L. Spíchal, Z. Pěkná,
O. Šamajová, K. Doležal, O. Plíhal, E. Benková, M. Strnad, L. Plíhalová, Phytochemistry
150 (2018) 1–11.
date_created: 2018-12-11T11:46:18Z
date_published: 2018-06-01T00:00:00Z
date_updated: 2023-09-11T12:53:11Z
day: '01'
department:
- _id: EvBe
doi: 10.1016/j.phytochem.2018.02.015
external_id:
isi:
- '000435623400001'
intvolume: ' 150'
isi: 1
language:
- iso: eng
month: '06'
oa_version: None
page: 1-11
publication: Phytochemistry
publication_status: published
publisher: Elsevier
publist_id: '7422'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 150
year: '2018'
...
---
_id: '42'
abstract:
- lang: eng
text: Seeds derive from ovules upon fertilization and therefore the total number
of ovules determines the final seed yield, a fundamental trait in crop plants.
Among the factors that co-ordinate the process of ovule formation, the transcription
factors CUP-SHAPED COTYLEDON 1 (CUC1) and CUC2 and the hormone cytokinin (CK)
have a particularly prominent role. Indeed, the absence of both CUC1 and CUC2
causes a severe reduction in ovule number, a phenotype that can be rescued by
CK treatment. In this study, we combined CK quantification with an integrative
genome-wide target identification approach to select Arabidopsis genes regulated
by CUCs that are also involved in CK metabolism. We focused our attention on the
functional characterization of UDP-GLUCOSYL TRANSFERASE 85A3 (UGT85A3) and UGT73C1,
which are up-regulated in the absence of CUC1 and CUC2 and encode enzymes able
to catalyse CK inactivation by O-glucosylation. Our results demonstrate a role
for these UGTs as a link between CUCs and CK homeostasis, and highlight the importance
of CUCs and CKs in the determination of seed yield.
acknowledgement: This work was funded by the Ministry of Education, Youth and Sports
of the Czech Republic through the National Program of Sustainability (grant no.
LO1204).
article_processing_charge: No
author:
- first_name: Mara
full_name: Cucinotta, Mara
last_name: Cucinotta
- first_name: Silvia
full_name: Manrique, Silvia
last_name: Manrique
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Ondřej
full_name: Novák, Ondřej
last_name: Novák
- first_name: Lucia
full_name: Colombo, Lucia
last_name: Colombo
citation:
ama: Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. Cup-shaped
Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number
in arabidopsis. Journal of Experimental Botany. 2018;69(21):5169-5176.
doi:10.1093/jxb/ery281
apa: Cucinotta, M., Manrique, S., Cuesta, C., Benková, E., Novák, O., & Colombo,
L. (2018). Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis
to determine ovule number in arabidopsis. Journal of Experimental Botany.
Oxford University Press. https://doi.org/10.1093/jxb/ery281
chicago: Cucinotta, Mara, Silvia Manrique, Candela Cuesta, Eva Benková, Ondřej Novák,
and Lucia Colombo. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin Homeostasis
to Determine Ovule Number in Arabidopsis.” Journal of Experimental Botany.
Oxford University Press, 2018. https://doi.org/10.1093/jxb/ery281.
ieee: M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, and L. Colombo,
“Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine
ovule number in arabidopsis,” Journal of Experimental Botany, vol. 69,
no. 21. Oxford University Press, pp. 5169–5176, 2018.
ista: Cucinotta M, Manrique S, Cuesta C, Benková E, Novák O, Colombo L. 2018. Cup-shaped
Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine ovule number
in arabidopsis. Journal of Experimental Botany. 69(21), 5169–5176.
mla: Cucinotta, Mara, et al. “Cup-Shaped Cotyledon1 (CUC1) and CU2 Regulate Cytokinin
Homeostasis to Determine Ovule Number in Arabidopsis.” Journal of Experimental
Botany, vol. 69, no. 21, Oxford University Press, 2018, pp. 5169–76, doi:10.1093/jxb/ery281.
short: M. Cucinotta, S. Manrique, C. Cuesta, E. Benková, O. Novák, L. Colombo, Journal
of Experimental Botany 69 (2018) 5169–5176.
date_created: 2018-12-11T11:44:19Z
date_published: 2018-07-26T00:00:00Z
date_updated: 2023-09-11T12:52:03Z
day: '26'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.1093/jxb/ery281
external_id:
isi:
- '000448163900015'
file:
- access_level: open_access
checksum: ca3b6711040b1662488aeb3d1f961f13
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T10:44:16Z
date_updated: 2020-07-14T12:46:25Z
file_id: '5691'
file_name: 2018_JournalExperimBotany_Cucinotta.pdf
file_size: 1292128
relation: main_file
file_date_updated: 2020-07-14T12:46:25Z
has_accepted_license: '1'
intvolume: ' 69'
isi: 1
issue: '21'
language:
- iso: eng
month: '07'
oa: 1
oa_version: Published Version
page: 5169 - 5176
publication: Journal of Experimental Botany
publication_status: published
publisher: Oxford University Press
publist_id: '8012'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cup-shaped Cotyledon1 (CUC1) and CU2 regulate cytokinin homeostasis to determine
ovule number in arabidopsis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 69
year: '2018'
...
---
_id: '946'
abstract:
- lang: eng
text: Roots navigate through soil integrating environmental signals to orient their
growth. The Arabidopsis root is a widely used model for developmental, physiological
and cell biological studies. Live imaging greatly aids these efforts, but the
horizontal sample position and continuous root tip displacement present significant
difficulties. Here, we develop a confocal microscope setup for vertical sample
mounting and integrated directional illumination. We present TipTracker – a custom
software for automatic tracking of diverse moving objects usable on various microscope
setups. Combined, this enables observation of root tips growing along the natural
gravity vector over prolonged periods of time, as well as the ability to induce
rapid gravity or light stimulation. We also track migrating cells in the developing
zebrafish embryo, demonstrating the utility of this system in the acquisition
of high-resolution data sets of dynamic samples. We provide detailed descriptions
of the tools enabling the easy implementation on other microscopes.
acknowledged_ssus:
- _id: M-Shop
- _id: Bio
acknowledgement: "Funding: Marie Curie Actions (FP7/2007-2013 no 291734) to Daniel
von Wangenheim; Austrian Science Fund (M 2128-B21) to Matyáš Fendrych; Austrian
Science Fund (FWF01_I1774S) to Eva Benková; European Research Council (FP7/2007-2013
no 282300) to Jiří Friml. \r\nThe authors are grateful to the Miba Machine Shop
at IST Austria for their contribution to the microscope setup and to Yvonne Kemper
for reading, understanding and correcting the manuscript.\r\n#BioimagingFacility"
article_number: e26792
article_processing_charge: Yes
author:
- first_name: Daniel
full_name: Von Wangenheim, Daniel
id: 49E91952-F248-11E8-B48F-1D18A9856A87
last_name: Von Wangenheim
orcid: 0000-0002-6862-1247
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Matyas
full_name: Fendrych, Matyas
id: 43905548-F248-11E8-B48F-1D18A9856A87
last_name: Fendrych
orcid: 0000-0002-9767-8699
- first_name: Vanessa
full_name: Barone, Vanessa
id: 419EECCC-F248-11E8-B48F-1D18A9856A87
last_name: Barone
orcid: 0000-0003-2676-3367
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. Live
tracking of moving samples in confocal microscopy for vertically grown roots.
eLife. 2017;6. doi:10.7554/eLife.26792
apa: von Wangenheim, D., Hauschild, R., Fendrych, M., Barone, V., Benková, E., &
Friml, J. (2017). Live tracking of moving samples in confocal microscopy for vertically
grown roots. ELife. eLife Sciences Publications. https://doi.org/10.7554/eLife.26792
chicago: Wangenheim, Daniel von, Robert Hauschild, Matyas Fendrych, Vanessa Barone,
Eva Benková, and Jiří Friml. “Live Tracking of Moving Samples in Confocal Microscopy
for Vertically Grown Roots.” ELife. eLife Sciences Publications, 2017.
https://doi.org/10.7554/eLife.26792.
ieee: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, and J.
Friml, “Live tracking of moving samples in confocal microscopy for vertically
grown roots,” eLife, vol. 6. eLife Sciences Publications, 2017.
ista: von Wangenheim D, Hauschild R, Fendrych M, Barone V, Benková E, Friml J. 2017.
Live tracking of moving samples in confocal microscopy for vertically grown roots.
eLife. 6, e26792.
mla: von Wangenheim, Daniel, et al. “Live Tracking of Moving Samples in Confocal
Microscopy for Vertically Grown Roots.” ELife, vol. 6, e26792, eLife Sciences
Publications, 2017, doi:10.7554/eLife.26792.
short: D. von Wangenheim, R. Hauschild, M. Fendrych, V. Barone, E. Benková, J. Friml,
ELife 6 (2017).
date_created: 2018-12-11T11:49:21Z
date_published: 2017-06-19T00:00:00Z
date_updated: 2025-05-07T11:12:33Z
day: '19'
ddc:
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department:
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- _id: EvBe
doi: 10.7554/eLife.26792
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month: '06'
oa: 1
oa_version: Published Version
project:
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call_identifier: FP7
grant_number: '291734'
name: International IST Postdoc Fellowship Programme
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call_identifier: FWF
grant_number: M02128
name: Molecular basis of root growth inhibition by auxin
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: eLife
publication_status: published
publisher: eLife Sciences Publications
publist_id: '6471'
pubrep_id: '847'
quality_controlled: '1'
related_material:
record:
- id: '5566'
relation: popular_science
status: public
scopus_import: '1'
status: public
title: Live tracking of moving samples in confocal microscopy for vertically grown
roots
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 6
year: '2017'
...
---
_id: '1004'
abstract:
- lang: eng
text: The fundamental tasks of the root system are, besides anchoring, mediating
interactions between plant and soil and providing the plant with water and nutrients.
The architecture of the root system is controlled by endogenous mechanisms that
constantly integrate environmental signals, such as availability of nutrients
and water. Extremely important for efficient soil exploitation and survival under
less favorable conditions is the developmental flexibility of the root system
that is largely determined by its postembryonic branching capacity. Modulation
of initiation and outgrowth of lateral roots provides roots with an exceptional
plasticity, allows optimal adjustment to underground heterogeneity, and enables
effective soil exploitation and use of resources. Here we discuss recent advances
in understanding the molecular mechanisms that shape the plant root system and
integrate external cues to adapt to the changing environment.
article_processing_charge: No
author:
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Ötvös K, Benková E. Spatiotemporal mechanisms of root branching. Current
Opinion in Genetics & Development. 2017;45:82-89. doi:10.1016/j.gde.2017.03.010
apa: Ötvös, K., & Benková, E. (2017). Spatiotemporal mechanisms of root branching.
Current Opinion in Genetics & Development. Elsevier. https://doi.org/10.1016/j.gde.2017.03.010
chicago: Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.”
Current Opinion in Genetics & Development. Elsevier, 2017. https://doi.org/10.1016/j.gde.2017.03.010.
ieee: K. Ötvös and E. Benková, “Spatiotemporal mechanisms of root branching,” Current
Opinion in Genetics & Development, vol. 45. Elsevier, pp. 82–89, 2017.
ista: Ötvös K, Benková E. 2017. Spatiotemporal mechanisms of root branching. Current
Opinion in Genetics & Development. 45, 82–89.
mla: Ötvös, Krisztina, and Eva Benková. “Spatiotemporal Mechanisms of Root Branching.”
Current Opinion in Genetics & Development, vol. 45, Elsevier, 2017,
pp. 82–89, doi:10.1016/j.gde.2017.03.010.
short: K. Ötvös, E. Benková, Current Opinion in Genetics & Development 45 (2017)
82–89.
date_created: 2018-12-11T11:49:38Z
date_published: 2017-08-01T00:00:00Z
date_updated: 2023-09-22T09:48:15Z
day: '01'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.1016/j.gde.2017.03.010
external_id:
isi:
- '000404880400013'
pmid:
- '28391060'
file:
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content_type: application/pdf
creator: dernst
date_created: 2019-04-17T08:00:36Z
date_updated: 2019-04-17T08:00:36Z
file_id: '6336'
file_name: Otvos_Benkova_CurOpDevBiol_2017.pdf
file_size: 364133
relation: main_file
success: 1
file_date_updated: 2019-04-17T08:00:36Z
has_accepted_license: '1'
intvolume: ' 45'
isi: 1
language:
- iso: eng
month: '08'
oa: 1
oa_version: Submitted Version
page: 82 - 89
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: Current Opinion in Genetics & Development
publication_identifier:
issn:
- 0959437X
publication_status: published
publisher: Elsevier
publist_id: '6394'
pubrep_id: '1017'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Spatiotemporal mechanisms of root branching
tmp:
image: /images/cc_by_nc_nd.png
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name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 45
year: '2017'
...