[{"day":"23","has_accepted_license":"1","intvolume":"        11","citation":{"ieee":"À. Gómez Sicilia, M. K. Sikora, M. Cieplak, and M. Carrión Vázquez, “An exploration of the universe of polyglutamine structures,” <i>PLoS Computational Biology</i>, vol. 11, no. 10. Public Library of Science, 2015.","mla":"Gómez Sicilia, Àngel, et al. “An Exploration of the Universe of Polyglutamine Structures.” <i>PLoS Computational Biology</i>, vol. 11, no. 10, e1004541, Public Library of Science, 2015, doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">10.1371/journal.pcbi.1004541</a>.","ista":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. 2015. An exploration of the universe of polyglutamine structures. PLoS Computational Biology. 11(10), e1004541.","chicago":"Gómez Sicilia, Àngel, Mateusz K Sikora, Marek Cieplak, and Mariano Carrión Vázquez. “An Exploration of the Universe of Polyglutamine Structures.” <i>PLoS Computational Biology</i>. Public Library of Science, 2015. <a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">https://doi.org/10.1371/journal.pcbi.1004541</a>.","apa":"Gómez Sicilia, À., Sikora, M. K., Cieplak, M., &#38; Carrión Vázquez, M. (2015). An exploration of the universe of polyglutamine structures. <i>PLoS Computational Biology</i>. Public Library of Science. <a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">https://doi.org/10.1371/journal.pcbi.1004541</a>","ama":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. An exploration of the universe of polyglutamine structures. <i>PLoS Computational Biology</i>. 2015;11(10). doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541\">10.1371/journal.pcbi.1004541</a>","short":"À. Gómez Sicilia, M.K. Sikora, M. Cieplak, M. Carrión Vázquez, PLoS Computational Biology 11 (2015)."},"article_number":"e1004541","status":"public","abstract":[{"lang":"eng","text":"Deposits of misfolded proteins in the human brain are associated with the development of many neurodegenerative diseases. Recent studies show that these proteins have common traits even at the monomer level. Among them, a polyglutamine region that is present in huntingtin is known to exhibit a correlation between the length of the chain and the severity as well as the earliness of the onset of Huntington disease. Here, we apply bias exchange molecular dynamics to generate structures of polyglutamine expansions of several lengths and characterize the resulting independent conformations. We compare the properties of these conformations to those of the standard proteins, as well as to other homopolymeric tracts. We find that, similar to the previously studied polyvaline chains, the set of possible transient folds is much broader than the set of known-to-date folds, although the conformations have different structures. We show that the mechanical stability is not related to any simple geometrical characteristics of the structures. We demonstrate that long polyglutamine expansions result in higher mechanical stability than the shorter ones. They also have a longer life span and are substantially more prone to form knotted structures. The knotted region has an average length of 35 residues, similar to the typical threshold for most polyglutamine-related diseases. Similarly, changes in shape and mechanical stability appear once the total length of the peptide exceeds this threshold of 35 glutamine residues. We suggest that knotted conformers may also harm the cellular machinery and thus lead to disease."}],"department":[{"_id":"CaHe"}],"acknowledgement":"We acknowledge the support by the EU Joint Programme in Neurodegenerative Diseases (JPND AC14/00037) project. The project is supported through the following funding organisations under the aegis of JPND—www.jpnd.eu: Ireland, HRB; Poland, National Science Centre; and Spain, ISCIII. ","pubrep_id":"478","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"10","_id":"1566","oa":1,"related_material":{"record":[{"relation":"research_data","status":"public","id":"9714"}]},"title":"An exploration of the universe of polyglutamine structures","publication_status":"published","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"scopus_import":1,"quality_controlled":"1","date_created":"2018-12-11T11:52:45Z","year":"2015","date_updated":"2023-02-23T14:05:55Z","volume":11,"file":[{"creator":"system","file_size":1412511,"checksum":"8b67d729be663bfc9af04bfd94459655","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:45:02Z","content_type":"application/pdf","file_id":"5207","date_created":"2018-12-12T10:16:21Z","file_name":"IST-2016-478-v1+1_journal.pcbi.1004541.pdf"}],"oa_version":"Published Version","date_published":"2015-10-23T00:00:00Z","issue":"10","publist_id":"5605","publication":"PLoS Computational Biology","ddc":["570"],"author":[{"last_name":"Gómez Sicilia","full_name":"Gómez Sicilia, Àngel","first_name":"Àngel"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","first_name":"Mateusz K","full_name":"Sikora, Mateusz K","last_name":"Sikora"},{"last_name":"Cieplak","full_name":"Cieplak, Marek","first_name":"Marek"},{"full_name":"Carrión Vázquez, Mariano","first_name":"Mariano","last_name":"Carrión Vázquez"}],"doi":"10.1371/journal.pcbi.1004541","publisher":"Public Library of Science","language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:45:02Z","type":"journal_article"},{"date_updated":"2022-08-25T13:56:10Z","quality_controlled":"1","page":"431 - 432","date_created":"2018-12-11T11:52:50Z","year":"2015","volume":161,"abstract":[{"lang":"eng","text":"In animal embryos, morphogen gradients determine tissue patterning and morphogenesis. Shyer et al. provide evidence that, during vertebrate gut formation, tissue folding generates graded activity of signals required for subsequent steps of gut growth and differentiation, thereby revealing an intriguing link between tissue morphogenesis and morphogen gradient formation."}],"status":"public","article_processing_charge":"No","day":"23","scopus_import":"1","citation":{"mla":"Bollenbach, Mark Tobias, and Carl-Philipp J. Heisenberg. “Gradients Are Shaping Up.” <i>Cell</i>, vol. 161, no. 3, Cell Press, 2015, pp. 431–32, doi:<a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">10.1016/j.cell.2015.04.009</a>.","ieee":"M. T. Bollenbach and C.-P. J. Heisenberg, “Gradients are shaping up,” <i>Cell</i>, vol. 161, no. 3. Cell Press, pp. 431–432, 2015.","ista":"Bollenbach MT, Heisenberg C-PJ. 2015. Gradients are shaping up. Cell. 161(3), 431–432.","apa":"Bollenbach, M. T., &#38; Heisenberg, C.-P. J. (2015). Gradients are shaping up. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">https://doi.org/10.1016/j.cell.2015.04.009</a>","chicago":"Bollenbach, Mark Tobias, and Carl-Philipp J Heisenberg. “Gradients Are Shaping Up.” <i>Cell</i>. Cell Press, 2015. <a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">https://doi.org/10.1016/j.cell.2015.04.009</a>.","ama":"Bollenbach MT, Heisenberg C-PJ. Gradients are shaping up. <i>Cell</i>. 2015;161(3):431-432. doi:<a href=\"https://doi.org/10.1016/j.cell.2015.04.009\">10.1016/j.cell.2015.04.009</a>","short":"M.T. Bollenbach, C.-P.J. Heisenberg, Cell 161 (2015) 431–432."},"intvolume":"       161","publisher":"Cell Press","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","_id":"1581","doi":"10.1016/j.cell.2015.04.009","month":"04","type":"journal_article","title":"Gradients are shaping up","language":[{"iso":"eng"}],"publication_status":"published","date_published":"2015-04-23T00:00:00Z","department":[{"_id":"ToBo"},{"_id":"CaHe"}],"oa_version":"None","author":[{"orcid":"0000-0003-4398-476X","id":"3E6DB97A-F248-11E8-B48F-1D18A9856A87","last_name":"Bollenbach","full_name":"Bollenbach, Mark Tobias","first_name":"Mark Tobias"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"issue":"3","publist_id":"5590","publication":"Cell"},{"citation":{"short":"S. Porazinski, H. Wang, Y. Asaoka, M. Behrndt, T. Miyamoto, H. Morita, S. Hata, T. Sasaki, G. Krens, Y. Osada, S. Asaka, A. Momoi, S. Linton, J. Miesfeld, B. Link, T. Senga, A. Castillo Morales, A. Urrutia, N. Shimizu, H. Nagase, S. Matsuura, S. Bagby, H. Kondoh, H. Nishina, C.-P.J. Heisenberg, M. Furutani Seiki, Nature 521 (2015) 217–221.","ama":"Porazinski S, Wang H, Asaoka Y, et al. YAP is essential for tissue tension to ensure vertebrate 3D body shape. <i>Nature</i>. 2015;521(7551):217-221. doi:<a href=\"https://doi.org/10.1038/nature14215\">10.1038/nature14215</a>","chicago":"Porazinski, Sean, Huijia Wang, Yoichi Asaoka, Martin Behrndt, Tatsuo Miyamoto, Hitoshi Morita, Shoji Hata, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” <i>Nature</i>. Nature Publishing Group, 2015. <a href=\"https://doi.org/10.1038/nature14215\">https://doi.org/10.1038/nature14215</a>.","ista":"Porazinski S, Wang H, Asaoka Y, Behrndt M, Miyamoto T, Morita H, Hata S, Sasaki T, Krens G, Osada Y, Asaka S, Momoi A, Linton S, Miesfeld J, Link B, Senga T, Castillo Morales A, Urrutia A, Shimizu N, Nagase H, Matsuura S, Bagby S, Kondoh H, Nishina H, Heisenberg C-PJ, Furutani Seiki M. 2015. YAP is essential for tissue tension to ensure vertebrate 3D body shape. Nature. 521(7551), 217–221.","apa":"Porazinski, S., Wang, H., Asaoka, Y., Behrndt, M., Miyamoto, T., Morita, H., … Furutani Seiki, M. (2015). YAP is essential for tissue tension to ensure vertebrate 3D body shape. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature14215\">https://doi.org/10.1038/nature14215</a>","mla":"Porazinski, Sean, et al. “YAP Is Essential for Tissue Tension to Ensure Vertebrate 3D Body Shape.” <i>Nature</i>, vol. 521, no. 7551, Nature Publishing Group, 2015, pp. 217–21, doi:<a href=\"https://doi.org/10.1038/nature14215\">10.1038/nature14215</a>.","ieee":"S. Porazinski <i>et al.</i>, “YAP is essential for tissue tension to ensure vertebrate 3D body shape,” <i>Nature</i>, vol. 521, no. 7551. Nature Publishing Group, pp. 217–221, 2015."},"intvolume":"       521","day":"16","abstract":[{"lang":"eng","text":"Vertebrates have a unique 3D body shape in which correct tissue and organ shape and alignment are essential for function. For example, vision requires the lens to be centred in the eye cup which must in turn be correctly positioned in the head. Tissue morphogenesis depends on force generation, force transmission through the tissue, and response of tissues and extracellular matrix to force. Although a century ago D'Arcy Thompson postulated that terrestrial animal body shapes are conditioned by gravity, there has been no animal model directly demonstrating how the aforementioned mechano-morphogenetic processes are coordinated to generate a body shape that withstands gravity. Here we report a unique medaka fish (Oryzias latipes) mutant, hirame (hir), which is sensitive to deformation by gravity. hir embryos display a markedly flattened body caused by mutation of YAP, a nuclear executor of Hippo signalling that regulates organ size. We show that actomyosin-mediated tissue tension is reduced in hir embryos, leading to tissue flattening and tissue misalignment, both of which contribute to body flattening. By analysing YAP function in 3D spheroids of human cells, we identify the Rho GTPase activating protein ARHGAP18 as an effector of YAP in controlling tissue tension. Together, these findings reveal a previously unrecognised function of YAP in regulating tissue shape and alignment required for proper 3D body shape. Understanding this morphogenetic function of YAP could facilitate the use of embryonic stem cells to generate complex organs requiring correct alignment of multiple tissues. "}],"status":"public","department":[{"_id":"CaHe"}],"oa":1,"title":"YAP is essential for tissue tension to ensure vertebrate 3D body shape","publication_status":"published","user_id":"2EBD1598-F248-11E8-B48F-1D18A9856A87","_id":"1817","month":"03","scopus_import":1,"pmid":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720436/","open_access":"1"}],"volume":521,"date_updated":"2021-01-12T06:53:23Z","quality_controlled":"1","page":"217 - 221","date_created":"2018-12-11T11:54:10Z","year":"2015","author":[{"last_name":"Porazinski","full_name":"Porazinski, Sean","first_name":"Sean"},{"last_name":"Wang","full_name":"Wang, Huijia","first_name":"Huijia"},{"first_name":"Yoichi","full_name":"Asaoka, Yoichi","last_name":"Asaoka"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt"},{"last_name":"Miyamoto","full_name":"Miyamoto, Tatsuo","first_name":"Tatsuo"},{"id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","full_name":"Morita, Hitoshi","first_name":"Hitoshi","last_name":"Morita"},{"full_name":"Hata, Shoji","first_name":"Shoji","last_name":"Hata"},{"last_name":"Sasaki","first_name":"Takashi","full_name":"Sasaki, Takashi"},{"last_name":"Krens","first_name":"Gabriel","full_name":"Krens, Gabriel","id":"2B819732-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-4761-5996"},{"full_name":"Osada, Yumi","first_name":"Yumi","last_name":"Osada"},{"last_name":"Asaka","full_name":"Asaka, Satoshi","first_name":"Satoshi"},{"last_name":"Momoi","first_name":"Akihiro","full_name":"Momoi, Akihiro"},{"last_name":"Linton","full_name":"Linton, Sarah","first_name":"Sarah"},{"full_name":"Miesfeld, Joel","first_name":"Joel","last_name":"Miesfeld"},{"full_name":"Link, Brian","first_name":"Brian","last_name":"Link"},{"first_name":"Takeshi","full_name":"Senga, Takeshi","last_name":"Senga"},{"last_name":"Castillo Morales","first_name":"Atahualpa","full_name":"Castillo Morales, Atahualpa"},{"last_name":"Urrutia","full_name":"Urrutia, Araxi","first_name":"Araxi"},{"first_name":"Nobuyoshi","full_name":"Shimizu, Nobuyoshi","last_name":"Shimizu"},{"first_name":"Hideaki","full_name":"Nagase, Hideaki","last_name":"Nagase"},{"full_name":"Matsuura, Shinya","first_name":"Shinya","last_name":"Matsuura"},{"first_name":"Stefan","full_name":"Bagby, Stefan","last_name":"Bagby"},{"first_name":"Hisato","full_name":"Kondoh, Hisato","last_name":"Kondoh"},{"full_name":"Nishina, Hiroshi","first_name":"Hiroshi","last_name":"Nishina"},{"last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Makoto","full_name":"Furutani Seiki, Makoto","last_name":"Furutani Seiki"}],"publication":"Nature","issue":"7551","publist_id":"5289","date_published":"2015-03-16T00:00:00Z","oa_version":"Submitted Version","external_id":{"pmid":["25778702"]},"type":"journal_article","language":[{"iso":"eng"}],"publisher":"Nature Publishing Group","doi":"10.1038/nature14215"},{"author":[{"first_name":"Àngel","full_name":"Gómez Sicilia, Àngel","last_name":"Gómez Sicilia"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","full_name":"Sikora, Mateusz K","first_name":"Mateusz K"},{"last_name":"Cieplak","full_name":"Cieplak, Marek","first_name":"Marek"},{"first_name":"Mariano","full_name":"Carrión Vázquez, Mariano","last_name":"Carrión Vázquez"}],"date_published":"2015-10-23T00:00:00Z","department":[{"_id":"CaHe"}],"oa_version":"Published Version","type":"research_data_reference","title":"An exploration of the universe of polyglutamine structures - submission to PLOS journals","related_material":{"record":[{"id":"1566","relation":"used_in_publication","status":"public"}]},"publisher":"Public Library of Science ","user_id":"6785fbc1-c503-11eb-8a32-93094b40e1cf","month":"10","_id":"9714","doi":"10.1371/journal.pcbi.1004541.s001","citation":{"short":"À. Gómez Sicilia, M.K. Sikora, M. Cieplak, M. Carrión Vázquez, (2015).","ama":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. An exploration of the universe of polyglutamine structures - submission to PLOS journals. 2015. doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">10.1371/journal.pcbi.1004541.s001</a>","apa":"Gómez Sicilia, À., Sikora, M. K., Cieplak, M., &#38; Carrión Vázquez, M. (2015). An exploration of the universe of polyglutamine structures - submission to PLOS journals. Public Library of Science . <a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">https://doi.org/10.1371/journal.pcbi.1004541.s001</a>","ista":"Gómez Sicilia À, Sikora MK, Cieplak M, Carrión Vázquez M. 2015. An exploration of the universe of polyglutamine structures - submission to PLOS journals, Public Library of Science , <a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">10.1371/journal.pcbi.1004541.s001</a>.","chicago":"Gómez Sicilia, Àngel, Mateusz K Sikora, Marek Cieplak, and Mariano Carrión Vázquez. “An Exploration of the Universe of Polyglutamine Structures - Submission to PLOS Journals.” Public Library of Science , 2015. <a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">https://doi.org/10.1371/journal.pcbi.1004541.s001</a>.","ieee":"À. Gómez Sicilia, M. K. Sikora, M. Cieplak, and M. Carrión Vázquez, “An exploration of the universe of polyglutamine structures - submission to PLOS journals.” Public Library of Science , 2015.","mla":"Gómez Sicilia, Àngel, et al. <i>An Exploration of the Universe of Polyglutamine Structures - Submission to PLOS Journals</i>. Public Library of Science , 2015, doi:<a href=\"https://doi.org/10.1371/journal.pcbi.1004541.s001\">10.1371/journal.pcbi.1004541.s001</a>."},"article_processing_charge":"No","day":"23","status":"public","date_updated":"2023-02-23T10:04:35Z","year":"2015","date_created":"2021-07-23T12:05:28Z"},{"acknowledgement":"Grant Nr. 2011/01/N/ST3/02475","department":[{"_id":"CaHe"}],"date_published":"2014-05-01T00:00:00Z","oa_version":"None","author":[{"last_name":"Chwastyk","first_name":"Mateusz","full_name":"Chwastyk, Mateusz"},{"last_name":"Galera Prat","full_name":"Galera Prat, Albert","first_name":"Albert"},{"id":"2F74BCDE-F248-11E8-B48F-1D18A9856A87","last_name":"Sikora","full_name":"Sikora, Mateusz K","first_name":"Mateusz K"},{"last_name":"Gómez Sicilia","first_name":"Àngel","full_name":"Gómez Sicilia, Àngel"},{"first_name":"Mariano","full_name":"Carrión Vázquez, Mariano","last_name":"Carrión Vázquez"},{"full_name":"Cieplak, Marek","first_name":"Marek","last_name":"Cieplak"}],"publist_id":"5204","issue":"5","publication":"Proteins: Structure, Function and Bioinformatics","publisher":"Wiley-Blackwell","_id":"1891","doi":"10.1002/prot.24436","month":"05","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","type":"journal_article","language":[{"iso":"eng"}],"publication_status":"published","title":"Theoretical tests of the mechanical protection strategy in protein nanomechanics","day":"01","intvolume":"        82","citation":{"ama":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. Theoretical tests of the mechanical protection strategy in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. 2014;82(5):717-726. doi:<a href=\"https://doi.org/10.1002/prot.24436\">10.1002/prot.24436</a>","short":"M. Chwastyk, A. Galera Prat, M.K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, M. Cieplak, Proteins: Structure, Function and Bioinformatics 82 (2014) 717–726.","ieee":"M. Chwastyk, A. Galera Prat, M. K. Sikora, À. Gómez Sicilia, M. Carrión Vázquez, and M. Cieplak, “Theoretical tests of the mechanical protection strategy in protein nanomechanics,” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 82, no. 5. Wiley-Blackwell, pp. 717–726, 2014.","mla":"Chwastyk, Mateusz, et al. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>, vol. 82, no. 5, Wiley-Blackwell, 2014, pp. 717–26, doi:<a href=\"https://doi.org/10.1002/prot.24436\">10.1002/prot.24436</a>.","chicago":"Chwastyk, Mateusz, Albert Galera Prat, Mateusz K Sikora, Àngel Gómez Sicilia, Mariano Carrión Vázquez, and Marek Cieplak. “Theoretical Tests of the Mechanical Protection Strategy in Protein Nanomechanics.” <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/prot.24436\">https://doi.org/10.1002/prot.24436</a>.","apa":"Chwastyk, M., Galera Prat, A., Sikora, M. K., Gómez Sicilia, À., Carrión Vázquez, M., &#38; Cieplak, M. (2014). Theoretical tests of the mechanical protection strategy in protein nanomechanics. <i>Proteins: Structure, Function and Bioinformatics</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/prot.24436\">https://doi.org/10.1002/prot.24436</a>","ista":"Chwastyk M, Galera Prat A, Sikora MK, Gómez Sicilia À, Carrión Vázquez M, Cieplak M. 2014. Theoretical tests of the mechanical protection strategy in protein nanomechanics. Proteins: Structure, Function and Bioinformatics. 82(5), 717–726."},"scopus_import":1,"date_updated":"2021-01-12T06:53:52Z","year":"2014","date_created":"2018-12-11T11:54:34Z","page":"717 - 726","volume":82,"abstract":[{"text":"We provide theoretical tests of a novel experimental technique to determine mechanostability of proteins based on stretching a mechanically protected protein by single-molecule force spectroscopy. This technique involves stretching a homogeneous or heterogeneous chain of reference proteins (single-molecule markers) in which one of them acts as host to the guest protein under study. The guest protein is grafted into the host through genetic engineering. It is expected that unraveling of the host precedes the unraveling of the guest removing ambiguities in the reading of the force-extension patterns of the guest protein. We study examples of such systems within a coarse-grained structure-based model. We consider systems with various ratios of mechanostability for the host and guest molecules and compare them to experimental results involving cohesin I as the guest molecule. For a comparison, we also study the force-displacement patterns in proteins that are linked in a serial fashion. We find that the mechanostability of the guest is similar to that of the isolated or serially linked protein. We also demonstrate that the ideal configuration of this strategy would be one in which the host is much more mechanostable than the single-molecule markers. We finally show that it is troublesome to use the highly stable cystine knot proteins as a host to graft a guest in stretching studies because this would involve a cleaving procedure.","lang":"eng"}],"status":"public"},{"issue":"2","publist_id":"5195","publication":"Nature Cell Biology","author":[{"first_name":"Martin","full_name":"Behrndt, Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"oa_version":"None","date_published":"2014-01-31T00:00:00Z","department":[{"_id":"CaHe"}],"title":"Lateral junction dynamics lead the way out","publication_status":"published","language":[{"iso":"eng"}],"type":"journal_article","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","doi":"10.1038/ncb2913","_id":"1900","month":"01","publisher":"Nature Publishing Group","scopus_import":1,"intvolume":"        16","citation":{"short":"M. Behrndt, C.-P.J. Heisenberg, Nature Cell Biology 16 (2014) 127–129.","ama":"Behrndt M, Heisenberg C-PJ. Lateral junction dynamics lead the way out. <i>Nature Cell Biology</i>. 2014;16(2):127-129. doi:<a href=\"https://doi.org/10.1038/ncb2913\">10.1038/ncb2913</a>","ista":"Behrndt M, Heisenberg C-PJ. 2014. Lateral junction dynamics lead the way out. Nature Cell Biology. 16(2), 127–129.","chicago":"Behrndt, Martin, and Carl-Philipp J Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2014. <a href=\"https://doi.org/10.1038/ncb2913\">https://doi.org/10.1038/ncb2913</a>.","apa":"Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). Lateral junction dynamics lead the way out. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2913\">https://doi.org/10.1038/ncb2913</a>","ieee":"M. Behrndt and C.-P. J. Heisenberg, “Lateral junction dynamics lead the way out,” <i>Nature Cell Biology</i>, vol. 16, no. 2. Nature Publishing Group, pp. 127–129, 2014.","mla":"Behrndt, Martin, and Carl-Philipp J. Heisenberg. “Lateral Junction Dynamics Lead the Way Out.” <i>Nature Cell Biology</i>, vol. 16, no. 2, Nature Publishing Group, 2014, pp. 127–29, doi:<a href=\"https://doi.org/10.1038/ncb2913\">10.1038/ncb2913</a>."},"day":"31","status":"public","abstract":[{"text":"Epithelial cell layers need to be tightly regulated to maintain their integrity and correct function. Cell integration into epithelial sheets is now shown to depend on the N-WASP-regulated stabilization of cortical F-actin, which generates distinct patterns of apical-lateral contractility at E-cadherin-based cell-cell junctions.","lang":"eng"}],"volume":16,"page":"127 - 129","quality_controlled":"1","year":"2014","date_created":"2018-12-11T11:54:37Z","date_updated":"2021-01-12T06:53:56Z"},{"date_created":"2018-12-11T11:54:41Z","year":"2014","quality_controlled":"1","page":"774 - 783","date_updated":"2023-09-07T12:05:08Z","volume":31,"article_processing_charge":"No","pmid":1,"main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pubmed/25535919","open_access":"1"}],"scopus_import":"1","doi":"10.1016/j.devcel.2014.11.003","publisher":"Cell Press","language":[{"iso":"eng"}],"type":"journal_article","external_id":{"pmid":["25535919"]},"oa_version":"Published Version","date_published":"2014-12-22T00:00:00Z","publication":"Developmental Cell","publist_id":"5182","issue":"6","author":[{"id":"2E3E0988-F248-11E8-B48F-1D18A9856A87","full_name":"Compagnon, Julien","first_name":"Julien","last_name":"Compagnon"},{"orcid":"0000-0003-2676-3367","id":"419EECCC-F248-11E8-B48F-1D18A9856A87","full_name":"Barone, Vanessa","first_name":"Vanessa","last_name":"Barone"},{"full_name":"Rajshekar, Srivarsha","first_name":"Srivarsha","last_name":"Rajshekar"},{"last_name":"Kottmeier","first_name":"Rita","full_name":"Kottmeier, Rita"},{"full_name":"Pranjic-Ferscha, Kornelija","first_name":"Kornelija","last_name":"Pranjic-Ferscha","id":"4362B3C2-F248-11E8-B48F-1D18A9856A87"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"status":"public","abstract":[{"lang":"eng","text":"Kupffer's vesicle (KV) is the zebrafish organ of laterality, patterning the embryo along its left-right (LR) axis. Regional differences in cell shape within the lumen-lining KV epithelium are essential for its LR patterning function. However, the processes by which KV cells acquire their characteristic shapes are largely unknown. Here, we show that the notochord induces regional differences in cell shape within KV by triggering extracellular matrix (ECM) accumulation adjacent to anterior-dorsal (AD) regions of KV. This localized ECM deposition restricts apical expansion of lumen-lining epithelial cells in AD regions of KV during lumen growth. Our study provides mechanistic insight into the processes by which KV translates global embryonic patterning into regional cell shape differences required for its LR symmetry-breaking function."}],"day":"22","citation":{"ama":"Compagnon J, Barone V, Rajshekar S, et al. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental Cell</i>. 2014;31(6):774-783. doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">10.1016/j.devcel.2014.11.003</a>","short":"J. Compagnon, V. Barone, S. Rajshekar, R. Kottmeier, K. Pranjic-Ferscha, M. Behrndt, C.-P.J. Heisenberg, Developmental Cell 31 (2014) 774–783.","mla":"Compagnon, Julien, et al. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>, vol. 31, no. 6, Cell Press, 2014, pp. 774–83, doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">10.1016/j.devcel.2014.11.003</a>.","ieee":"J. Compagnon <i>et al.</i>, “The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ,” <i>Developmental Cell</i>, vol. 31, no. 6. Cell Press, pp. 774–783, 2014.","apa":"Compagnon, J., Barone, V., Rajshekar, S., Kottmeier, R., Pranjic-Ferscha, K., Behrndt, M., &#38; Heisenberg, C.-P. J. (2014). The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">https://doi.org/10.1016/j.devcel.2014.11.003</a>","ista":"Compagnon J, Barone V, Rajshekar S, Kottmeier R, Pranjic-Ferscha K, Behrndt M, Heisenberg C-PJ. 2014. The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ. Developmental Cell. 31(6), 774–783.","chicago":"Compagnon, Julien, Vanessa Barone, Srivarsha Rajshekar, Rita Kottmeier, Kornelija Pranjic-Ferscha, Martin Behrndt, and Carl-Philipp J Heisenberg. “The Notochord Breaks Bilateral Symmetry by Controlling Cell Shapes in the Zebrafish Laterality Organ.” <i>Developmental Cell</i>. Cell Press, 2014. <a href=\"https://doi.org/10.1016/j.devcel.2014.11.003\">https://doi.org/10.1016/j.devcel.2014.11.003</a>."},"intvolume":"        31","month":"12","_id":"1912","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","title":"The notochord breaks bilateral symmetry by controlling cell shapes in the Zebrafish laterality organ","oa":1,"related_material":{"record":[{"id":"961","status":"public","relation":"dissertation_contains"}]},"department":[{"_id":"CaHe"}],"acknowledgement":"We are grateful to members of the C.-P.H. lab, M. Concha, D. Siekhaus, and J. Vermot for comments on the manuscript and to M. Furutani-Seiki for sharing reagents. This work was supported by the Institute of Science and Technology Austria and an Alexander von Humboldt Foundation fellowship to J.C."},{"file":[{"file_name":"IST-2016-429-v1+1_document.pdf","file_id":"5202","date_created":"2018-12-12T10:16:16Z","content_type":"application/pdf","access_level":"open_access","date_updated":"2020-07-14T12:45:21Z","relation":"main_file","checksum":"8dbe81ec656bf1264d8889bda9b2b985","file_size":941387,"creator":"system"}],"volume":16,"date_created":"2018-12-11T11:54:44Z","year":"2014","quality_controlled":"1","date_updated":"2021-01-12T06:54:06Z","scopus_import":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2020-07-14T12:45:21Z","doi":"10.1088/1367-2630/16/6/065005","publisher":"IOP Publishing Ltd.","publication":"New Journal of Physics","publist_id":"5171","author":[{"first_name":"Hélène","full_name":"Berthoumieux, Hélène","last_name":"Berthoumieux"},{"last_name":"Maître","first_name":"Jean-Léon","full_name":"Maître, Jean-Léon","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-3688-1474"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"},{"full_name":"Paluch, Ewa","first_name":"Ewa","last_name":"Paluch"},{"last_name":"Julicher","full_name":"Julicher, Frank","first_name":"Frank"},{"first_name":"Guillaume","full_name":"Salbreux, Guillaume","last_name":"Salbreux"}],"ddc":["570"],"oa_version":"Published Version","date_published":"2014-06-01T00:00:00Z","status":"public","article_number":"065005","abstract":[{"text":"We derive the equations for a thin, axisymmetric elastic shell subjected to an internal active stress giving rise to active tension and moments within the shell. We discuss the stability of a cylindrical elastic shell and its response to a localized change in internal active stress. This description is relevant to describe the cellular actomyosin cortex, a thin shell at the cell surface behaving elastically at a short timescale and subjected to active internal forces arising from myosin molecular motor activity. We show that the recent observations of cell deformation following detachment of adherent cells (Maître J-L et al 2012 Science 338 253-6) are well accounted for by this mechanical description. The actin cortex elastic and bending moduli can be obtained from a quantitative analysis of cell shapes observed in these experiments. Our approach thus provides a non-invasive, imaging-based method for the extraction of cellular physical parameters.","lang":"eng"}],"intvolume":"        16","citation":{"ieee":"H. Berthoumieux, J.-L. Maître, C.-P. J. Heisenberg, E. Paluch, F. Julicher, and G. Salbreux, “Active elastic thin shell theory for cellular deformations,” <i>New Journal of Physics</i>, vol. 16. IOP Publishing Ltd., 2014.","mla":"Berthoumieux, Hélène, et al. “Active Elastic Thin Shell Theory for Cellular Deformations.” <i>New Journal of Physics</i>, vol. 16, 065005, IOP Publishing Ltd., 2014, doi:<a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">10.1088/1367-2630/16/6/065005</a>.","apa":"Berthoumieux, H., Maître, J.-L., Heisenberg, C.-P. J., Paluch, E., Julicher, F., &#38; Salbreux, G. (2014). Active elastic thin shell theory for cellular deformations. <i>New Journal of Physics</i>. IOP Publishing Ltd. <a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">https://doi.org/10.1088/1367-2630/16/6/065005</a>","chicago":"Berthoumieux, Hélène, Jean-Léon Maître, Carl-Philipp J Heisenberg, Ewa Paluch, Frank Julicher, and Guillaume Salbreux. “Active Elastic Thin Shell Theory for Cellular Deformations.” <i>New Journal of Physics</i>. IOP Publishing Ltd., 2014. <a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">https://doi.org/10.1088/1367-2630/16/6/065005</a>.","ista":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. 2014. Active elastic thin shell theory for cellular deformations. New Journal of Physics. 16, 065005.","ama":"Berthoumieux H, Maître J-L, Heisenberg C-PJ, Paluch E, Julicher F, Salbreux G. Active elastic thin shell theory for cellular deformations. <i>New Journal of Physics</i>. 2014;16. doi:<a href=\"https://doi.org/10.1088/1367-2630/16/6/065005\">10.1088/1367-2630/16/6/065005</a>","short":"H. Berthoumieux, J.-L. Maître, C.-P.J. Heisenberg, E. Paluch, F. Julicher, G. Salbreux, New Journal of Physics 16 (2014)."},"day":"01","has_accepted_license":"1","publication_status":"published","oa":1,"title":"Active elastic thin shell theory for cellular deformations","month":"06","_id":"1923","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","pubrep_id":"429","department":[{"_id":"CaHe"}]},{"department":[{"_id":"CaHe"},{"_id":"MiSi"}],"acknowledgement":"This work was supported by EC grant Marie Curie RTN-CT-2006-035616, CARBIO 'Carbon nanotubes for biomedical applications' and Austrian FFG grant mnt-era.net 823980, 'IntelliTip'.\r\n","month":"03","_id":"1925","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_status":"published","title":"A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes","oa":1,"article_type":"original","day":"28","has_accepted_license":"1","citation":{"short":"C. Lamprecht, B. Plochberger, V. Ruprecht, S. Wieser, C. Rankl, E. Heister, B. Unterauer, M. Brameshuber, J. Danzberger, P. Lukanov, E. Flahaut, G. Schütz, P. Hinterdorfer, A. Ebner, Nanotechnology 25 (2014).","ama":"Lamprecht C, Plochberger B, Ruprecht V, et al. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. 2014;25(12). doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>","chicago":"Lamprecht, Constanze, Birgit Plochberger, Verena Ruprecht, Stefan Wieser, Christian Rankl, Elena Heister, Barbara Unterauer, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>. IOP Publishing, 2014. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>.","apa":"Lamprecht, C., Plochberger, B., Ruprecht, V., Wieser, S., Rankl, C., Heister, E., … Ebner, A. (2014). A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. <i>Nanotechnology</i>. IOP Publishing. <a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">https://doi.org/10.1088/0957-4484/25/12/125704</a>","ista":"Lamprecht C, Plochberger B, Ruprecht V, Wieser S, Rankl C, Heister E, Unterauer B, Brameshuber M, Danzberger J, Lukanov P, Flahaut E, Schütz G, Hinterdorfer P, Ebner A. 2014. A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes. Nanotechnology. 25(12), 125704.","ieee":"C. Lamprecht <i>et al.</i>, “A single-molecule approach to explore binding uptake and transport of cancer cell targeting nanotubes,” <i>Nanotechnology</i>, vol. 25, no. 12. IOP Publishing, 2014.","mla":"Lamprecht, Constanze, et al. “A Single-Molecule Approach to Explore Binding Uptake and Transport of Cancer Cell Targeting Nanotubes.” <i>Nanotechnology</i>, vol. 25, no. 12, 125704, IOP Publishing, 2014, doi:<a href=\"https://doi.org/10.1088/0957-4484/25/12/125704\">10.1088/0957-4484/25/12/125704</a>."},"intvolume":"        25","status":"public","article_number":"125704","abstract":[{"lang":"eng","text":"In the past decade carbon nanotubes (CNTs) have been widely studied as a potential drug-delivery system, especially with functionality for cellular targeting. Yet, little is known about the actual process of docking to cell receptors and transport dynamics after internalization. Here we performed single-particle studies of folic acid (FA) mediated CNT binding to human carcinoma cells and their transport inside the cytosol. In particular, we employed molecular recognition force spectroscopy, an atomic force microscopy based method, to visualize and quantify docking of FA functionalized CNTs to FA binding receptors in terms of binding probability and binding force. We then traced individual fluorescently labeled, FA functionalized CNTs after specific uptake, and created a dynamic 'roadmap' that clearly showed trajectories of directed diffusion and areas of nanotube confinement in the cytosol. Our results demonstrate the potential of a single-molecule approach for investigation of drug-delivery vehicles and their targeting capacity."}],"oa_version":"Submitted Version","date_published":"2014-03-28T00:00:00Z","issue":"12","publication":"Nanotechnology","publist_id":"5169","author":[{"last_name":"Lamprecht","first_name":"Constanze","full_name":"Lamprecht, Constanze"},{"last_name":"Plochberger","first_name":"Birgit","full_name":"Plochberger, Birgit"},{"first_name":"Verena","full_name":"Ruprecht, Verena","last_name":"Ruprecht","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Stefan","full_name":"Wieser, Stefan","last_name":"Wieser","orcid":"0000-0002-2670-2217","id":"355AA5A0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rankl","first_name":"Christian","full_name":"Rankl, Christian"},{"last_name":"Heister","first_name":"Elena","full_name":"Heister, Elena"},{"last_name":"Unterauer","first_name":"Barbara","full_name":"Unterauer, Barbara"},{"last_name":"Brameshuber","first_name":"Mario","full_name":"Brameshuber, Mario"},{"full_name":"Danzberger, Jürgen","first_name":"Jürgen","last_name":"Danzberger"},{"full_name":"Lukanov, Petar","first_name":"Petar","last_name":"Lukanov"},{"full_name":"Flahaut, Emmanuel","first_name":"Emmanuel","last_name":"Flahaut"},{"last_name":"Schütz","full_name":"Schütz, Gerhard","first_name":"Gerhard"},{"last_name":"Hinterdorfer","first_name":"Peter","full_name":"Hinterdorfer, Peter"},{"full_name":"Ebner, Andreas","first_name":"Andreas","last_name":"Ebner"}],"ddc":["570"],"doi":"10.1088/0957-4484/25/12/125704","publisher":"IOP Publishing","language":[{"iso":"eng"}],"type":"journal_article","file_date_updated":"2020-07-14T12:45:21Z","article_processing_charge":"No","scopus_import":1,"year":"2014","date_created":"2018-12-11T11:54:45Z","date_updated":"2021-01-12T06:54:07Z","file":[{"creator":"dernst","file_size":3804152,"checksum":"df4e03d225a19179e7790f6d87a12332","relation":"main_file","access_level":"open_access","date_updated":"2020-07-14T12:45:21Z","content_type":"application/pdf","date_created":"2020-05-15T09:21:19Z","file_id":"7856","file_name":"2014_Nanotechnology_Lamprecht.pdf"}],"volume":25},{"status":"public","abstract":[{"text":"A variety of developmental and disease related processes depend on epithelial cell sheet spreading. In order to gain insight into the biophysical mechanism(s) underlying the tissue morphogenesis we studied the spreading of an epithelium during the early development of the zebrafish embryo. In zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium, spreads over the yolk cell to completely engulf it at the end of gastrulation. Previous studies have proposed that an actomyosin ring forming within the yolk syncytial layer (YSL) acts as purse string that through constriction along its circumference pulls on the margin of the EVL. Direct biophysical evidence for this hypothesis has however been missing. The aim of the thesis was to understand how the actomyosin ring may generate pulling forces onto the EVL and what cellular mechanism(s) may facilitate the spreading of the epithelium. Using laser ablation to measure cortical tension within the actomyosin ring we found an anisotropic tension distribution, which was highest along the circumference of the ring. However the low degree of anisotropy was incompatible with the actomyosin ring functioning as a purse string only. Additionally, we observed retrograde cortical flow from vegetal parts of the ring into the EVL margin. Interpreting the experimental data using a theoretical distribution that models  the tissues as active viscous gels led us to proposen that the actomyosin ring has a twofold contribution to EVL epiboly. It not only acts as a purse string through constriction along its circumference, but in addition constriction along the width of the ring generates pulling forces through friction-resisted cortical flow. Moreover, when rendering the purse string mechanism unproductive EVL epiboly proceeded normally indicating that the flow-friction mechanism is sufficient to drive the process. Aiming to understand what cellular mechanism(s) may facilitate the spreading of the epithelium we found that tension-oriented EVL cell divisions limit tissue anisotropy by releasing tension along the division axis and promote epithelial spreading. Notably, EVL cells undergo ectopic cell fusion in conditions in which oriented-cell division is impaired or the epithelium is mechanically challenged. Taken together our study of EVL epiboly suggests a novel mechanism of force generation for actomyosin rings through friction-resisted cortical flow and highlights the importance of tension-oriented cell divisions in epithelial morphogenesis.","lang":"eng"}],"page":"91","date_created":"2018-12-11T11:51:49Z","year":"2014","date_updated":"2023-10-17T12:16:58Z","acknowledged_ssus":[{"_id":"SSU"}],"supervisor":[{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"citation":{"ieee":"M. Behrndt, “Forces driving epithelial spreading in zebrafish epiboly,” IST Austria, 2014.","mla":"Behrndt, Martin. <i>Forces Driving Epithelial Spreading in Zebrafish Epiboly</i>. IST Austria, 2014.","ista":"Behrndt M. 2014. Forces driving epithelial spreading in zebrafish epiboly. IST Austria.","apa":"Behrndt, M. (2014). <i>Forces driving epithelial spreading in zebrafish epiboly</i>. IST Austria.","chicago":"Behrndt, Martin. “Forces Driving Epithelial Spreading in Zebrafish Epiboly.” IST Austria, 2014.","ama":"Behrndt M. Forces driving epithelial spreading in zebrafish epiboly. 2014.","short":"M. Behrndt, Forces Driving Epithelial Spreading in Zebrafish Epiboly, IST Austria, 2014."},"day":"01","related_material":{"record":[{"id":"2282","relation":"part_of_dissertation","status":"public"},{"status":"public","relation":"part_of_dissertation","id":"2950"},{"relation":"part_of_dissertation","status":"public","id":"3373"}]},"title":"Forces driving epithelial spreading in zebrafish epiboly","language":[{"iso":"eng"}],"publication_status":"published","type":"dissertation","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"08","_id":"1403","publisher":"IST Austria","alternative_title":["IST Austria Thesis"],"publist_id":"5804","author":[{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","full_name":"Behrndt, Martin","first_name":"Martin"}],"oa_version":"None","date_published":"2014-08-01T00:00:00Z","department":[{"_id":"CaHe"}]},{"abstract":[{"text":"Mechanically coupled cells can generate forces driving cell and tissue morphogenesis during development. Visualization and measuring of these forces is of major importance to better understand the complexity of the biomechanic processes that shape cells and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively assess mechanical tension in different tissues of the developing zebrafish and in cultures of primary germ layer progenitor cells ex vivo.","lang":"eng"}],"status":"public","editor":[{"first_name":"Celeste","full_name":"Nelson, Celeste","last_name":"Nelson"}],"citation":{"ama":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Nelson C, ed. <i>Tissue Morphogenesis</i>. Vol 1189. Methods in Molecular Biology. New York, NY: Springer; 2014:219-235. doi:<a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">10.1007/978-1-4939-1164-6_15</a>","short":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:, C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, NY, 2014, pp. 219–235.","ieee":"M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg, “UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo,” in <i>Tissue Morphogenesis</i>, vol. 1189, C. Nelson, Ed. New York, NY: Springer, 2014, pp. 219–235.","mla":"Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” <i>Tissue Morphogenesis</i>, edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:<a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">10.1007/978-1-4939-1164-6_15</a>.","ista":"Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In: Tissue Morphogenesis. vol. 1189, 219–235.","chicago":"Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In <i>Tissue Morphogenesis</i>, edited by Celeste Nelson, 1189:219–35. Methods in Molecular Biology. New York, NY: Springer, 2014. <a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">https://doi.org/10.1007/978-1-4939-1164-6_15</a>.","apa":"Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., &#38; Heisenberg, C.-P. J. (2014). UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), <i>Tissue Morphogenesis</i> (Vol. 1189, pp. 219–235). New York, NY: Springer. <a href=\"https://doi.org/10.1007/978-1-4939-1164-6_15\">https://doi.org/10.1007/978-1-4939-1164-6_15</a>"},"intvolume":"      1189","day":"22","publication_status":"published","title":"UV laser ablation to measure cell and tissue-generated forces in the zebrafish embryo in vivo and ex vivo","place":"New York, NY","_id":"6178","month":"08","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","department":[{"_id":"CaHe"}],"volume":1189,"date_updated":"2023-09-05T14:12:00Z","date_created":"2019-03-26T08:55:59Z","year":"2014","series_title":"Methods in Molecular Biology","page":"219-235","quality_controlled":"1","pmid":1,"publication_identifier":{"eissn":["1940-6029"],"issn":["1064-3745"],"isbn":["9781493911639","9781493911646"]},"article_processing_charge":"No","type":"book_chapter","language":[{"iso":"eng"}],"publisher":"Springer","doi":"10.1007/978-1-4939-1164-6_15","author":[{"id":"3FE6E4E8-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5920-9090","first_name":"Michael","full_name":"Smutny, Michael","last_name":"Smutny"},{"id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87","last_name":"Behrndt","full_name":"Behrndt, Martin","first_name":"Martin"},{"full_name":"Campinho, Pedro","first_name":"Pedro","last_name":"Campinho","orcid":"0000-0002-8526-5416","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87","last_name":"Ruprecht","full_name":"Ruprecht, Verena","first_name":"Verena"},{"orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg"}],"publication":"Tissue Morphogenesis","date_published":"2014-08-22T00:00:00Z","oa_version":"None","external_id":{"pmid":["25245697"]}},{"article_processing_charge":"No","main_file_link":[{"url":"https://doi.org/10.1111/cga.12039","open_access":"1"}],"pmid":1,"scopus_import":"1","publication_identifier":{"issn":["0914-3505"]},"date_updated":"2022-03-04T08:26:05Z","date_created":"2022-03-04T08:17:25Z","year":"2014","page":"1-7","quality_controlled":"1","volume":54,"date_published":"2014-02-01T00:00:00Z","oa_version":"None","external_id":{"pmid":["24666178"]},"author":[{"last_name":"Hashimoto","full_name":"Hashimoto, Masakazu","first_name":"Masakazu"},{"last_name":"Morita","full_name":"Morita, Hitoshi","first_name":"Hitoshi","id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ueno","first_name":"Naoto","full_name":"Ueno, Naoto"}],"issue":"1","publication":"Congenital Anomalies","publisher":"Wiley","doi":"10.1111/cga.12039","type":"journal_article","language":[{"iso":"eng"}],"day":"01","intvolume":"        54","citation":{"short":"M. Hashimoto, H. Morita, N. Ueno, Congenital Anomalies 54 (2014) 1–7.","ama":"Hashimoto M, Morita H, Ueno N. Molecular and cellular mechanisms of development underlying congenital diseases. <i>Congenital Anomalies</i>. 2014;54(1):1-7. doi:<a href=\"https://doi.org/10.1111/cga.12039\">10.1111/cga.12039</a>","apa":"Hashimoto, M., Morita, H., &#38; Ueno, N. (2014). Molecular and cellular mechanisms of development underlying congenital diseases. <i>Congenital Anomalies</i>. Wiley. <a href=\"https://doi.org/10.1111/cga.12039\">https://doi.org/10.1111/cga.12039</a>","ista":"Hashimoto M, Morita H, Ueno N. 2014. Molecular and cellular mechanisms of development underlying congenital diseases. Congenital Anomalies. 54(1), 1–7.","chicago":"Hashimoto, Masakazu, Hitoshi Morita, and Naoto Ueno. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” <i>Congenital Anomalies</i>. Wiley, 2014. <a href=\"https://doi.org/10.1111/cga.12039\">https://doi.org/10.1111/cga.12039</a>.","ieee":"M. Hashimoto, H. Morita, and N. Ueno, “Molecular and cellular mechanisms of development underlying congenital diseases,” <i>Congenital Anomalies</i>, vol. 54, no. 1. Wiley, pp. 1–7, 2014.","mla":"Hashimoto, Masakazu, et al. “Molecular and Cellular Mechanisms of Development Underlying Congenital Diseases.” <i>Congenital Anomalies</i>, vol. 54, no. 1, Wiley, 2014, pp. 1–7, doi:<a href=\"https://doi.org/10.1111/cga.12039\">10.1111/cga.12039</a>."},"keyword":["Developmental Biology","Embryology","General Medicine","Pediatrics","Perinatology","and Child Health"],"abstract":[{"lang":"eng","text":"In the last several decades, developmental biology has clarified the molecular mechanisms of embryogenesis and organogenesis. In particular, it has demonstrated that the “tool-kit genes” essential for regulating developmental processes are not only highly conserved among species, but are also used as systems at various times and places in an organism to control distinct developmental events. Therefore, mutations in many of these tool-kit genes may cause congenital diseases involving morphological abnormalities. This link between genes and abnormal morphological phenotypes underscores the importance of understanding how cells behave and contribute to morphogenesis as a result of gene function. Recent improvements in live imaging and in quantitative analyses of cellular dynamics will advance our understanding of the cellular pathogenesis of congenital diseases associated with aberrant morphologies. In these studies, it is critical to select an appropriate model organism for the particular phenomenon of interest."}],"status":"public","department":[{"_id":"CaHe"}],"acknowledgement":"The authors thank all the members of the Division of Morphogenesis, National Institute for Basic Biology, for their contributions to the research, their encouragement, and helpful discussions, particularly Dr M. Suzuki for his critical reading of the manuscript. We also thank the Model Animal Research and Spectrography and Bioimaging Facilities, NIBB Core Research Facilities, for technical support. M.H. was supported by a research fellowship from the Japan Society for the Promotion of Science (JSPS). Our work introduced in this review was supported by a Grant-in-Aid for Scientific Research on Innovative Areas from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan, to N.U.","_id":"10815","month":"02","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","publication_status":"published","oa":1,"title":"Molecular and cellular mechanisms of development underlying congenital diseases"},{"status":"public","volume":322,"abstract":[{"lang":"eng","text":"Avian forelimb digit homology remains one of the standard themes in comparative biology and EvoDevo research. In order to resolve the apparent contradictions between embryological and paleontological evidence a variety of hypotheses have been presented in recent years. The proposals range from excluding birds from the dinosaur clade, to assignments of homology by different criteria, or even assuming a hexadactyl tetrapod limb ground state. At present two approaches prevail: the frame shift hypothesis and the pyramid reduction hypothesis. While the former postulates a homeotic shift of digit identities, the latter argues for a gradual bilateral reduction of phalanges and digits. Here we present a new model that integrates elements from both hypotheses with the existing experimental and fossil evidence. We start from the main feature common to both earlier concepts, the initiating ontogenetic event: reduction and loss of the anterior-most digit. It is proposed that a concerted mechanism of molecular regulation and developmental mechanics is capable of shifting the boundaries of hoxD expression in embryonic forelimb buds as well as changing the digit phenotypes. Based on a distinction between positional (topological) and compositional (phenotypic) homology criteria, we argue that the identity of the avian digits is II, III, IV, despite a partially altered phenotype. Finally, we introduce an alternative digit reduction scheme that reconciles the current fossil evidence with the presented molecular-morphogenetic model. Our approach identifies specific experiments that allow to test whether gene expression can be shifted and digit phenotypes can be altered by induced digit loss or digit gain."}],"year":"2014","date_created":"2018-12-11T11:56:33Z","page":"1 - 12","quality_controlled":"1","date_updated":"2021-01-12T06:56:16Z","publication_identifier":{"issn":["15525007"]},"intvolume":"       322","citation":{"mla":"Capek, Daniel, et al. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>, vol. 322, no. 1, Wiley-Blackwell, 2014, pp. 1–12, doi:<a href=\"https://doi.org/10.1002/jez.b.22545\">10.1002/jez.b.22545</a>.","ieee":"D. Capek, B. Metscher, and G. Müller, “Thumbs down: A molecular-morphogenetic approach to avian digit homology,” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>, vol. 322, no. 1. Wiley-Blackwell, pp. 1–12, 2014.","apa":"Capek, D., Metscher, B., &#38; Müller, G. (2014). Thumbs down: A molecular-morphogenetic approach to avian digit homology. <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/jez.b.22545\">https://doi.org/10.1002/jez.b.22545</a>","chicago":"Capek, Daniel, Brian Metscher, and Gerd Müller. “Thumbs down: A Molecular-Morphogenetic Approach to Avian Digit Homology.” <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1002/jez.b.22545\">https://doi.org/10.1002/jez.b.22545</a>.","ista":"Capek D, Metscher B, Müller G. 2014. Thumbs down: A molecular-morphogenetic approach to avian digit homology. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 322(1), 1–12.","ama":"Capek D, Metscher B, Müller G. Thumbs down: A molecular-morphogenetic approach to avian digit homology. <i>Journal of Experimental Zoology Part B: Molecular and Developmental Evolution</i>. 2014;322(1):1-12. doi:<a href=\"https://doi.org/10.1002/jez.b.22545\">10.1002/jez.b.22545</a>","short":"D. Capek, B. Metscher, G. Müller, Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 322 (2014) 1–12."},"scopus_import":1,"day":"01","language":[{"iso":"eng"}],"publication_status":"published","title":"Thumbs down: A molecular-morphogenetic approach to avian digit homology","type":"journal_article","_id":"2248","doi":"10.1002/jez.b.22545","month":"01","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","publisher":"Wiley-Blackwell","issue":"1","publication":"Journal of Experimental Zoology Part B: Molecular and Developmental Evolution","publist_id":"4701","author":[{"id":"31C42484-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-5199-9940","first_name":"Daniel","full_name":"Capek, Daniel","last_name":"Capek"},{"full_name":"Metscher, Brian","first_name":"Brian","last_name":"Metscher"},{"last_name":"Müller","first_name":"Gerd","full_name":"Müller, Gerd"}],"oa_version":"None","department":[{"_id":"CaHe"}],"date_published":"2014-01-01T00:00:00Z"},{"publication_identifier":{"issn":["2663-337X"]},"supervisor":[{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J"}],"citation":{"mla":"Campinho, Pedro. <i>Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading</i>. Institute of Science and Technology Austria, 2013.","ieee":"P. Campinho, “Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading,” Institute of Science and Technology Austria, 2013.","apa":"Campinho, P. (2013). <i>Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading</i>. Institute of Science and Technology Austria.","ista":"Campinho P. 2013. Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading. Institute of Science and Technology Austria.","chicago":"Campinho, Pedro. “Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading.” Institute of Science and Technology Austria, 2013.","ama":"Campinho P. Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading. 2013.","short":"P. Campinho, Mechanics of Zebrafish Epiboly: Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading, Institute of Science and Technology Austria, 2013."},"day":"01","article_processing_charge":"No","status":"public","abstract":[{"lang":"eng","text":"Epithelial spreading is a critical part of various developmental and wound repair processes. Here we use zebrafish epiboly as a model system to study the cellular and molecular mechanisms underlying the spreading of epithelial sheets. During zebrafish epiboly the enveloping cell layer (EVL), a simple squamous epithelium, spreads over the embryo to eventually cover the entire yolk cell by the end of gastrulation. The EVL leading edge is anchored through tight junctions to the yolk syncytial layer (YSL), where directly adjacent to the EVL margin a contractile actomyosin ring is formed that is thought to drive EVL epiboly. The prevalent view in the field was that the contractile ring exerts a pulling force on the EVL margin, which pulls the EVL towards the vegetal pole. However, how this force is generated and how it affects EVL morphology still remains elusive. Moreover, the cellular mechanisms mediating the increase in EVL surface area, while maintaining tissue integrity and function are still unclear. Here we show that the YSL actomyosin ring pulls on the EVL margin by two distinct force-generating mechanisms. One mechanism is based on contraction of the ring around its circumference, as previously proposed. The second mechanism is based on actomyosin retrogade flows, generating force through resistance against the substrate. The latter can function at any epiboly stage even in situations where the contraction-based mechanism is unproductive. Additionally, we demonstrate that during epiboly the EVL is subjected to anisotropic tension, which guides the orientation of EVL cell division along the main axis (animal-vegetal) of tension. The influence of tension in cell division orientation involves cell elongation and requires myosin-2 activity for proper spindle alignment. Strikingly, we reveal that tension-oriented cell divisions release anisotropic tension within the EVL and that in the absence of such divisions, EVL cells undergo ectopic fusions. We conclude that forces applied to the EVL by the action of the YSL actomyosin ring generate a tension anisotropy in the EVL that orients cell divisions, which in turn limit tissue tension increase thereby facilitating tissue spreading."}],"year":"2013","degree_awarded":"PhD","date_created":"2018-12-11T11:51:50Z","page":"123","acknowledged_ssus":[{"_id":"Bio"},{"_id":"PreCl"}],"date_updated":"2023-09-07T11:36:07Z","publist_id":"5801","alternative_title":["ISTA Thesis"],"author":[{"id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8526-5416","last_name":"Campinho","full_name":"Campinho, Pedro","first_name":"Pedro"}],"oa_version":"None","department":[{"_id":"CaHe"}],"date_published":"2013-10-01T00:00:00Z","language":[{"iso":"eng"}],"publication_status":"published","title":"Mechanics of zebrafish epiboly: Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading","type":"dissertation","_id":"1406","month":"10","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publisher":"Institute of Science and Technology Austria"},{"day":"01","scopus_import":1,"intvolume":"       126","citation":{"short":"R. Pérez Gómez, J. Slovakova, N. Rives Quinto, A. Krejčí, A. Carmena, Journal of Cell Science 126 (2013) 4873–4884.","ama":"Pérez Gómez R, Slovakova J, Rives Quinto N, Krejčí A, Carmena A. A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. <i>Journal of Cell Science</i>. 2013;126(21):4873-4884. doi:<a href=\"https://doi.org/10.1242/jcs.125617\">10.1242/jcs.125617</a>","apa":"Pérez Gómez, R., Slovakova, J., Rives Quinto, N., Krejčí, A., &#38; Carmena, A. (2013). A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. <i>Journal of Cell Science</i>. Company of Biologists. <a href=\"https://doi.org/10.1242/jcs.125617\">https://doi.org/10.1242/jcs.125617</a>","ista":"Pérez Gómez R, Slovakova J, Rives Quinto N, Krejčí A, Carmena A. 2013. A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development. Journal of Cell Science. 126(21), 4873–4884.","chicago":"Pérez Gómez, Raquel, Jana Slovakova, Noemí Rives Quinto, Alena Krejčí, and Ana Carmena. “A Serrate-Notch-Canoe Complex Mediates Essential Interactions between Glia and Neuroepithelial Cells during Drosophila Optic Lobe Development.” <i>Journal of Cell Science</i>. Company of Biologists, 2013. <a href=\"https://doi.org/10.1242/jcs.125617\">https://doi.org/10.1242/jcs.125617</a>.","mla":"Pérez Gómez, Raquel, et al. “A Serrate-Notch-Canoe Complex Mediates Essential Interactions between Glia and Neuroepithelial Cells during Drosophila Optic Lobe Development.” <i>Journal of Cell Science</i>, vol. 126, no. 21, Company of Biologists, 2013, pp. 4873–84, doi:<a href=\"https://doi.org/10.1242/jcs.125617\">10.1242/jcs.125617</a>.","ieee":"R. Pérez Gómez, J. Slovakova, N. Rives Quinto, A. Krejčí, and A. Carmena, “A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development,” <i>Journal of Cell Science</i>, vol. 126, no. 21. Company of Biologists, pp. 4873–4884, 2013."},"quality_controlled":"1","page":"4873 - 4884","date_created":"2018-12-11T11:56:43Z","year":"2013","date_updated":"2021-01-12T06:56:29Z","status":"public","volume":126,"abstract":[{"lang":"eng","text":"It is firmly established that interactions between neurons and glia are fundamental across species for the correct establishment of a functional brain. Here, we found that the glia of the Drosophila larval brain display an essential non-autonomous role during the development of the optic lobe. The optic lobe develops from neuroepithelial cells that proliferate by dividing symmetrically until they switch to asymmetric/differentiative divisions that generate neuroblasts. The proneural gene lethal of scute (l9sc) is transiently activated by the epidermal growth factor receptor (EGFR)-Ras signal transduction pathway at the leading edge of a proneural wave that sweeps from medial to lateral neuroepithelium, promoting this switch. This process is tightly regulated by the tissue-autonomous function within the neuroepithelium of multiple signaling pathways, including EGFR-Ras and Notch. This study shows that the Notch ligand Serrate (Ser) is expressed in the glia and it forms a complex in vivo with Notch and Canoe, which colocalize at the adherens junctions of neuroepithelial cells. This complex is crucial for interactions between glia and neuroepithelial cells during optic lobe development. Ser is tissue-autonomously required in the glia where it activates Notch to regulate its proliferation, and non-autonomously in the neuroepithelium where Ser induces Notch signaling to avoid the premature activation of the EGFR-Ras pathway and hence of L9sc. Interestingly, different Notch activity reporters showed very different expression patterns in the glia and in the neuroepithelium, suggesting the existence of tissue-specific factors that promote the expression of particular Notch target genes or/and a reporter response dependent on different thresholds of Notch signaling."}],"oa_version":"None","date_published":"2013-11-01T00:00:00Z","department":[{"_id":"CaHe"}],"issue":"21","publist_id":"4658","publication":"Journal of Cell Science","author":[{"last_name":"Pérez Gómez","full_name":"Pérez Gómez, Raquel","first_name":"Raquel"},{"last_name":"Slovakova","full_name":"Slovakova, Jana","first_name":"Jana","id":"30F3F2F0-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Rives Quinto","first_name":"Noemí","full_name":"Rives Quinto, Noemí"},{"first_name":"Alena","full_name":"Krejčí, Alena","last_name":"Krejčí"},{"last_name":"Carmena","full_name":"Carmena, Ana","first_name":"Ana"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"11","_id":"2278","doi":"10.1242/jcs.125617","publisher":"Company of Biologists","title":"A serrate-notch-canoe complex mediates essential interactions between glia and neuroepithelial cells during Drosophila optic lobe development","publication_status":"published","language":[{"iso":"eng"}],"type":"journal_article"},{"volume":15,"date_updated":"2023-02-21T17:02:44Z","project":[{"call_identifier":"FWF","grant_number":"I 930-B20","name":"Control of Epithelial Cell Layer Spreading in Zebrafish","_id":"252ABD0A-B435-11E9-9278-68D0E5697425"}],"acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"quality_controlled":"1","page":"1405 - 1414","date_created":"2018-12-11T11:56:45Z","year":"2013","scopus_import":1,"main_file_link":[{"open_access":"1","url":"http://hal.upmc.fr/hal-00983313/"}],"type":"journal_article","language":[{"iso":"eng"}],"publisher":"Nature Publishing Group","doi":"10.1038/ncb2869","author":[{"orcid":"0000-0002-8526-5416","id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","full_name":"Campinho, Pedro","first_name":"Pedro","last_name":"Campinho"},{"full_name":"Behrndt, Martin","first_name":"Martin","last_name":"Behrndt","id":"3ECECA3A-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ranft","first_name":"Jonas","full_name":"Ranft, Jonas"},{"full_name":"Risler, Thomas","first_name":"Thomas","last_name":"Risler"},{"full_name":"Minc, Nicolas","first_name":"Nicolas","last_name":"Minc"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"}],"publication":"Nature Cell Biology","publist_id":"4652","date_published":"2013-11-10T00:00:00Z","oa_version":"Submitted Version","abstract":[{"lang":"eng","text":"Epithelial spreading is a common and fundamental aspect of various developmental and disease-related processes such as epithelial closure and wound healing. A key challenge for epithelial tissues undergoing spreading is to increase their surface area without disrupting epithelial integrity. Here we show that orienting cell divisions by tension constitutes an efficient mechanism by which the enveloping cell layer (EVL) releases anisotropic tension while undergoing spreading during zebrafish epiboly. The control of EVL cell-division orientation by tension involves cell elongation and requires myosin II activity to align the mitotic spindle with the main tension axis. We also found that in the absence of tension-oriented cell divisions and in the presence of increased tissue tension, EVL cells undergo ectopic fusions, suggesting that the reduction of tension anisotropy by oriented cell divisions is required to prevent EVL cells from fusing. We conclude that cell-division orientation by tension constitutes a key mechanism for limiting tension anisotropy and thus promoting tissue spreading during EVL epiboly."}],"status":"public","intvolume":"        15","citation":{"apa":"Campinho, P., Behrndt, M., Ranft, J., Risler, T., Minc, N., &#38; Heisenberg, C.-P. J. (2013). Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/ncb2869\">https://doi.org/10.1038/ncb2869</a>","ista":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. 2013. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. Nature Cell Biology. 15, 1405–1414.","chicago":"Campinho, Pedro, Martin Behrndt, Jonas Ranft, Thomas Risler, Nicolas Minc, and Carl-Philipp J Heisenberg. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell Biology</i>. Nature Publishing Group, 2013. <a href=\"https://doi.org/10.1038/ncb2869\">https://doi.org/10.1038/ncb2869</a>.","mla":"Campinho, Pedro, et al. “Tension-Oriented Cell Divisions Limit Anisotropic Tissue Tension in Epithelial Spreading during Zebrafish Epiboly.” <i>Nature Cell Biology</i>, vol. 15, Nature Publishing Group, 2013, pp. 1405–14, doi:<a href=\"https://doi.org/10.1038/ncb2869\">10.1038/ncb2869</a>.","ieee":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, and C.-P. J. Heisenberg, “Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly,” <i>Nature Cell Biology</i>, vol. 15. Nature Publishing Group, pp. 1405–1414, 2013.","short":"P. Campinho, M. Behrndt, J. Ranft, T. Risler, N. Minc, C.-P.J. Heisenberg, Nature Cell Biology 15 (2013) 1405–1414.","ama":"Campinho P, Behrndt M, Ranft J, Risler T, Minc N, Heisenberg C-PJ. Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly. <i>Nature Cell Biology</i>. 2013;15:1405-1414. doi:<a href=\"https://doi.org/10.1038/ncb2869\">10.1038/ncb2869</a>"},"day":"10","related_material":{"record":[{"id":"1403","relation":"dissertation_contains","status":"public"}]},"oa":1,"title":"Tension-oriented cell divisions limit anisotropic tissue tension in epithelial spreading during zebrafish epiboly","publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"11","_id":"2282","department":[{"_id":"CaHe"}],"acknowledgement":"This work was supported by the IST Austria and MPI-CBG "},{"date_published":"2013-10-04T00:00:00Z","external_id":{"pmid":["24097062"]},"oa_version":"Submitted Version","author":[{"id":"3AFBBC42-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8526-5416","full_name":"Campinho, Pedro","first_name":"Pedro","last_name":"Campinho"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"issue":"21","publist_id":"4645","publication":"EMBO Journal","publisher":"Wiley-Blackwell","doi":"10.1038/emboj.2013.225","type":"journal_article","language":[{"iso":"eng"}],"scopus_import":1,"main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3817470/","open_access":"1"}],"pmid":1,"date_updated":"2021-01-12T06:56:32Z","page":"2783 - 2784","quality_controlled":"1","year":"2013","date_created":"2018-12-11T11:56:46Z","volume":32,"department":[{"_id":"CaHe"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"10","_id":"2286","oa":1,"title":"The force and effect of cell proliferation","publication_status":"published","day":"04","citation":{"short":"P. Campinho, C.-P.J. Heisenberg, EMBO Journal 32 (2013) 2783–2784.","ama":"Campinho P, Heisenberg C-PJ. The force and effect of cell proliferation. <i>EMBO Journal</i>. 2013;32(21):2783-2784. doi:<a href=\"https://doi.org/10.1038/emboj.2013.225\">10.1038/emboj.2013.225</a>","chicago":"Campinho, Pedro, and Carl-Philipp J Heisenberg. “The Force and Effect of Cell Proliferation.” <i>EMBO Journal</i>. Wiley-Blackwell, 2013. <a href=\"https://doi.org/10.1038/emboj.2013.225\">https://doi.org/10.1038/emboj.2013.225</a>.","apa":"Campinho, P., &#38; Heisenberg, C.-P. J. (2013). The force and effect of cell proliferation. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1038/emboj.2013.225\">https://doi.org/10.1038/emboj.2013.225</a>","ista":"Campinho P, Heisenberg C-PJ. 2013. The force and effect of cell proliferation. EMBO Journal. 32(21), 2783–2784.","mla":"Campinho, Pedro, and Carl-Philipp J. Heisenberg. “The Force and Effect of Cell Proliferation.” <i>EMBO Journal</i>, vol. 32, no. 21, Wiley-Blackwell, 2013, pp. 2783–84, doi:<a href=\"https://doi.org/10.1038/emboj.2013.225\">10.1038/emboj.2013.225</a>.","ieee":"P. Campinho and C.-P. J. Heisenberg, “The force and effect of cell proliferation,” <i>EMBO Journal</i>, vol. 32, no. 21. Wiley-Blackwell, pp. 2783–2784, 2013."},"intvolume":"        32","abstract":[{"text":"The spatiotemporal control of cell divisions is a key factor in epithelial morphogenesis and patterning. Mao et al (2013) now describe how differential rates of proliferation within the Drosophila wing disc epithelium give rise to anisotropic tissue tension in peripheral/proximal regions of the disc. Such global tissue tension anisotropy in turn determines the orientation of cell divisions by controlling epithelial cell elongation.","lang":"eng"}],"status":"public"},{"citation":{"short":"J.-L. Maître, C.-P.J. Heisenberg, Current Biology 23 (2013) R626–R633.","ama":"Maître J-L, Heisenberg C-PJ. Three functions of cadherins in cell adhesion. <i>Current Biology</i>. 2013;23(14):R626-R633. doi:<a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">10.1016/j.cub.2013.06.019</a>","apa":"Maître, J.-L., &#38; Heisenberg, C.-P. J. (2013). Three functions of cadherins in cell adhesion. <i>Current Biology</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">https://doi.org/10.1016/j.cub.2013.06.019</a>","ista":"Maître J-L, Heisenberg C-PJ. 2013. Three functions of cadherins in cell adhesion. Current Biology. 23(14), R626–R633.","chicago":"Maître, Jean-Léon, and Carl-Philipp J Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” <i>Current Biology</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">https://doi.org/10.1016/j.cub.2013.06.019</a>.","ieee":"J.-L. Maître and C.-P. J. Heisenberg, “Three functions of cadherins in cell adhesion,” <i>Current Biology</i>, vol. 23, no. 14. Cell Press, pp. R626–R633, 2013.","mla":"Maître, Jean-Léon, and Carl-Philipp J. Heisenberg. “Three Functions of Cadherins in Cell Adhesion.” <i>Current Biology</i>, vol. 23, no. 14, Cell Press, 2013, pp. R626–33, doi:<a href=\"https://doi.org/10.1016/j.cub.2013.06.019\">10.1016/j.cub.2013.06.019</a>."},"intvolume":"        23","day":"22","has_accepted_license":"1","status":"public","abstract":[{"lang":"eng","text":"Cadherins are transmembrane proteins that mediate cell–cell adhesion in animals. By regulating contact formation and stability, cadherins play a crucial role in tissue morphogenesis and homeostasis. Here, we review the three major  unctions of cadherins in cell–cell contact formation and stability. Two of those functions lead to a decrease in interfacial ension at the forming cell–cell contact, thereby promoting contact expansion — first, by providing adhesion tension that lowers interfacial tension at the cell–cell contact, and second, by signaling to the actomyosin cytoskeleton in order to reduce cortex tension and thus interfacial tension at the contact. The third function of cadherins in cell–cell contact formation is to stabilize the contact by resisting mechanical forces that pull on the contact."}],"department":[{"_id":"CaHe"}],"title":"Three functions of cadherins in cell adhesion","oa":1,"publication_status":"published","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","_id":"2469","month":"07","scopus_import":1,"pmid":1,"tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","short":"CC BY (4.0)"},"volume":23,"file":[{"file_id":"5881","content_type":"application/pdf","date_created":"2019-01-24T15:40:22Z","date_updated":"2020-07-14T12:45:41Z","access_level":"open_access","file_name":"2013_CurrentBiology_Maitre.pdf","file_size":247320,"creator":"dernst","relation":"main_file","checksum":"6a424b2f007b41d4955a9135793b2162"}],"quality_controlled":"1","page":"R626 - R633","date_created":"2018-12-11T11:57:51Z","year":"2013","date_updated":"2021-01-12T06:57:40Z","publist_id":"4433","issue":"14","publication":"Current Biology","ddc":["570"],"author":[{"full_name":"Maître, Jean-Léon","first_name":"Jean-Léon","last_name":"Maître","orcid":"0000-0002-3688-1474","id":"48F1E0D8-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","last_name":"Heisenberg","first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J"}],"oa_version":"Published Version","external_id":{"pmid":["23885883"]},"date_published":"2013-07-22T00:00:00Z","language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:45:41Z","type":"journal_article","doi":"10.1016/j.cub.2013.06.019","publisher":"Cell Press"},{"status":"public","volume":153,"abstract":[{"lang":"eng","text":"During development, mechanical forces cause changes in size, shape, number, position, and gene expression of cells. They are therefore integral to any morphogenetic processes. Force generation by actin-myosin networks and force transmission through adhesive complexes are two self-organizing phenomena driving tissue morphogenesis. Coordination and integration of forces by long-range force transmission and mechanosensing of cells within tissues produce large-scale tissue shape changes. Extrinsic mechanical forces also control tissue patterning by modulating cell fate specification and differentiation. Thus, the interplay between tissue mechanics and biochemical signaling orchestrates tissue morphogenesis and patterning in development."}],"quality_controlled":"1","page":"948 - 962","year":"2013","date_created":"2018-12-11T11:59:50Z","date_updated":"2021-01-12T07:00:04Z","scopus_import":1,"intvolume":"       153","citation":{"chicago":"Heisenberg, Carl-Philipp J, and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” <i>Cell</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">https://doi.org/10.1016/j.cell.2013.05.008</a>.","apa":"Heisenberg, C.-P. J., &#38; Bellaïche, Y. (2013). Forces in tissue morphogenesis and patterning. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">https://doi.org/10.1016/j.cell.2013.05.008</a>","ista":"Heisenberg C-PJ, Bellaïche Y. 2013. Forces in tissue morphogenesis and patterning. Cell. 153(5), 948–962.","ieee":"C.-P. J. Heisenberg and Y. Bellaïche, “Forces in tissue morphogenesis and patterning,” <i>Cell</i>, vol. 153, no. 5. Cell Press, pp. 948–962, 2013.","mla":"Heisenberg, Carl-Philipp J., and Yohanns Bellaïche. “Forces in Tissue Morphogenesis and Patterning.” <i>Cell</i>, vol. 153, no. 5, Cell Press, 2013, pp. 948–62, doi:<a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">10.1016/j.cell.2013.05.008</a>.","short":"C.-P.J. Heisenberg, Y. Bellaïche, Cell 153 (2013) 948–962.","ama":"Heisenberg C-PJ, Bellaïche Y. Forces in tissue morphogenesis and patterning. <i>Cell</i>. 2013;153(5):948-962. doi:<a href=\"https://doi.org/10.1016/j.cell.2013.05.008\">10.1016/j.cell.2013.05.008</a>"},"day":"23","title":"Forces in tissue morphogenesis and patterning","language":[{"iso":"eng"}],"publication_status":"published","type":"journal_article","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"05","_id":"2833","doi":"10.1016/j.cell.2013.05.008","publisher":"Cell Press","publist_id":"3966","issue":"5","publication":"Cell","author":[{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566"},{"full_name":"Bellaïche, Yohanns","first_name":"Yohanns","last_name":"Bellaïche"}],"oa_version":"None","date_published":"2013-05-23T00:00:00Z","acknowledgement":"C.-P.H. is supported by the Institute of Science and Technology Austria and grants from the Deutsche Forschungsgemeinschaft (DFG) and Fonds zur Förderung der wissenschaftlichen Forschung (FWF).","department":[{"_id":"CaHe"}]},{"date_updated":"2021-01-12T07:00:09Z","quality_controlled":"1","page":"567 - 569","year":"2013","date_created":"2018-12-11T11:59:52Z","abstract":[{"text":"In zebrafish early development, blastoderm cells undergo extensive radial intercalations, triggering the spreading of the blastoderm over the yolk cell and thereby initiating embryonic body axis formation. Now reporting in Developmental Cell, Song et al. (2013) demonstrate a critical function for EGF-dependent E-cadherin endocytosis in promoting blastoderm cell intercalations.","lang":"eng"}],"volume":24,"status":"public","day":"25","scopus_import":1,"citation":{"ama":"Morita H, Heisenberg C-PJ. Holding on and letting go: Cadherin turnover in cell intercalation. <i>Developmental Cell</i>. 2013;24(6):567-569. doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">10.1016/j.devcel.2013.03.007</a>","short":"H. Morita, C.-P.J. Heisenberg, Developmental Cell 24 (2013) 567–569.","mla":"Morita, Hitoshi, and Carl-Philipp J. Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” <i>Developmental Cell</i>, vol. 24, no. 6, Cell Press, 2013, pp. 567–69, doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">10.1016/j.devcel.2013.03.007</a>.","ieee":"H. Morita and C.-P. J. Heisenberg, “Holding on and letting go: Cadherin turnover in cell intercalation,” <i>Developmental Cell</i>, vol. 24, no. 6. Cell Press, pp. 567–569, 2013.","ista":"Morita H, Heisenberg C-PJ. 2013. Holding on and letting go: Cadherin turnover in cell intercalation. Developmental Cell. 24(6), 567–569.","chicago":"Morita, Hitoshi, and Carl-Philipp J Heisenberg. “Holding on and Letting Go: Cadherin Turnover in Cell Intercalation.” <i>Developmental Cell</i>. Cell Press, 2013. <a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">https://doi.org/10.1016/j.devcel.2013.03.007</a>.","apa":"Morita, H., &#38; Heisenberg, C.-P. J. (2013). Holding on and letting go: Cadherin turnover in cell intercalation. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2013.03.007\">https://doi.org/10.1016/j.devcel.2013.03.007</a>"},"intvolume":"        24","publisher":"Cell Press","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"05","_id":"2841","doi":"10.1016/j.devcel.2013.03.007","type":"journal_article","title":"Holding on and letting go: Cadherin turnover in cell intercalation","publication_status":"published","language":[{"iso":"eng"}],"date_published":"2013-05-25T00:00:00Z","department":[{"_id":"CaHe"}],"oa_version":"None","author":[{"id":"4C6E54C6-F248-11E8-B48F-1D18A9856A87","first_name":"Hitoshi","full_name":"Morita, Hitoshi","last_name":"Morita"},{"first_name":"Carl-Philipp J","full_name":"Heisenberg, Carl-Philipp J","last_name":"Heisenberg","orcid":"0000-0002-0912-4566","id":"39427864-F248-11E8-B48F-1D18A9856A87"}],"publication":"Developmental Cell","publist_id":"3956","issue":"6"}]