LOCUS       CLT_FFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187682442.25
VERSION     urn.local...1608187682442.25
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1100..2224
                     /label="LacI LAA"
     terminator      2231..2335
                     /label="T1"
     misc_feature    2362^2363
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    2363..2442
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             2469..3125
                     /label="tetR"
     terminator      3132..3236
                     /label="T1"
     rep_origin      3283..5435
                     /label="SC101* Origin (Approx Position)"
     terminator      5478..5583
                     /label="t0"
     CDS             complement(5609..6403)
                     /label="KnR (neo)"
     misc_feature    6615^6616
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccatt caggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccata tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     1141 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     1201 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     1261 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     1321 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     1381 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     1441 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     1501 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     1561 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     1621 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     1681 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     1741 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     1801 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     1861 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     1921 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     1981 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     2041 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     2101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2161 acaggtttcc cgactggaaa gcgggcaggc agcaaacgac gaaaactacg ctttagcagc
     2221 ttaatctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2281 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagagccag
     2341 tgaggcacct ttcgtcttca cctcgagaat tgtgagcgga taacaattga cattgtgagc
     2401 ggataacaag atactgagca catcagcagg acgcactgac cgaattcatt aaagaggaga
     2461 aaggtaccat gtccagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
     2521 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
     2581 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
     2641 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
     2701 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
     2761 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
     2821 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
     2881 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
     2941 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
     3001 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
     3061 aaaaacaact taaatgtgaa agtgggtctg cagcaaacga cgaaaactac gctttagcag
     3121 cttaatctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3181 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagagcca
     3241 cctaggcgtc gacagatctc ccgggtaccg agctcgaatt cggctgttct ggtgttgcta
     3301 gtttgttatc agaatcgcag atccggcttc aggtttgccg gctgaaagcg ctatttcttc
     3361 cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc
     3421 tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta
     3481 acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact ggtttcacct
     3541 gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc atggtgaaca
     3601 gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt
     3661 tcatctgtgc atatggacag ttttcccttt gatatctaac ggtgaacagt tgttctactt
     3721 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
     3781 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     3841 cgaaccattg agatcatgct tactttgcat gtcactcaaa aattttgcct caaaactggt
     3901 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tacgtaggta
     3961 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     4021 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     4081 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     4141 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     4201 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     4261 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     4321 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     4381 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     4441 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     4501 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     4561 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     4621 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     4681 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     4741 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     4801 cccgggagat ctgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     4861 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     4921 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     4981 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     5041 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     5101 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     5161 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     5221 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     5281 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg
     5341 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac
     5401 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac aggcttaccc
     5461 gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     5521 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     5581 agcgagctct cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     5641 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     5701 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     5761 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     5821 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     5881 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     5941 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     6001 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     6061 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     6121 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     6181 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     6241 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     6301 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     6361 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     6421 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     6481 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     6541 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     6601 cctttcgtct tcacctcgag agtactgcca cggaggcacc tttcgtcttc acc
//
LOCUS       CLT_FFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187682422.24
VERSION     urn.local...1608187682422.24
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1100..2224
                     /label="LacI"
     terminator      complement(2238..2342)
                     /label="T1"
     CDS             complement(2349..3005)
                     /label="tetR"
     misc_feature    complement(3032..3111)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    3111^3112
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      3174..5326
                     /label="SC101* Origin (Approx Position)"
     terminator      5369..5474
                     /label="t0"
     CDS             complement(5500..6294)
                     /label="KnR (neo)"
     misc_feature    6506^6507
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccatt caggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccata tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     1141 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     1201 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     1261 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     1321 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     1381 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     1441 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     1501 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     1561 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     1621 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     1681 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     1741 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     1801 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     1861 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     1921 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     1981 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     2041 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     2101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2161 acaggtttcc cgactggaaa gcgggcaggc agcaaacgac gaaaactacg ctttagcagc
     2221 ttaagagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag cgttcaccga
     2281 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
     2341 cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcagaccc actttcacat
     2401 ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct
     2461 gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta
     2521 gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct
     2581 aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt
     2641 tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta
     2701 cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg
     2761 ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc
     2821 ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa
     2881 tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg
     2941 attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatctg
     3001 gacatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     3061 tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg aggtgaagac
     3121 gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     3181 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     3241 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     3301 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     3361 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     3421 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     3481 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     3541 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     3601 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     3661 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     3721 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     3781 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     3841 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     3901 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     3961 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     4021 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     4081 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     4141 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     4201 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     4261 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     4321 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     4381 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     4441 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     4501 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     4561 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     4621 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     4681 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     4741 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     4801 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     4861 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     4921 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     4981 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     5041 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     5101 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     5161 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     5221 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     5281 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     5341 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     5401 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     5461 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5521 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5581 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5641 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5701 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5761 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5821 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5881 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5941 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     6001 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     6061 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     6121 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     6181 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6241 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     6301 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     6361 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     6421 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6481 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     6541 cacc
//
LOCUS       CLT_FRR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608500807455.171
VERSION     urn.local...1608500807455.171
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..795)
                     /label="tetR"
     misc_feature    complement(822..901)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    901^902
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      964..3116
                     /label="SC101* Origin (Approx Position)"
     terminator      3159..3264
                     /label="t0"
     CDS             complement(3290..4084)
                     /label="KnR (neo)"
     misc_feature    4296^4297
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4334^4335
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4340..4413
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             4441..5187
                     /label="cI"
     terminator      5194..5298
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="lacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcagaccc
      181 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      241 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      301 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      361 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      421 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      481 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      541 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      601 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      661 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      721 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      781 atctaatctg gacatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg
      841 atgtgctcag tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg
      901 aggtgaagac gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat
      961 tcggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc
     1021 ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact
     1081 ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct
     1141 atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc
     1201 tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg
     1261 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
     1321 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa
     1381 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     1441 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     1501 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     1561 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     1621 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     1681 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     1741 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     1801 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     1861 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     1921 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     1981 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     2041 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     2101 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     2161 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     2221 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     2281 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     2341 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     2401 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     2461 atgataatta ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt
     2521 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     2581 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     2641 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     2701 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     2761 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     2821 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     2881 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     2941 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     3001 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     3061 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     3121 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     3181 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     3241 ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact
     3301 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     3361 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     3421 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     3481 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     3541 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     3601 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     3661 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     3721 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     3781 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     3841 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     3901 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     3961 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     4021 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     4081 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
     4141 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     4201 gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata
     4261 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac
     4321 ctttcgtctt cacctcgagt ccctatcagt gatagagatt gacatcccta tcagtgatag
     4381 agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc
     4441 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
     4501 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
     4561 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
     4621 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
     4681 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
     4741 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
     4801 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
     4861 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
     4921 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
     4981 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
     5041 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
     5101 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgcagcaaac
     5161 gacgaaaact acgctttagc agcttgatct agaggcatca aataaaacga aaggctcagt
     5221 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     5281 caaatccgcc gccctagagc cattcaggct cttaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       CLT_RFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187682392.21
VERSION     urn.local...1608187682392.21
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    43^44
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    44..123
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             152..1276
                     /label="LacI"
     terminator      1283..1387
                     /label="T1"
     misc_feature    1414^1415
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    1415..1494
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1521..2177
                     /label="TetR"
     terminator      2184..2288
                     /label="T1"
     rep_origin      2335..4487
                     /label="SC101* Origin (Approx Position)"
     terminator      4530..4635
                     /label="t0"
     CDS             complement(4661..5455)
                     /label="KnR (neo)"
     misc_feature    5667^5668
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5690..5794)
                     /label="T1"
     CDS             complement(5801..6547)
                     /label="cI"
     misc_feature    complement(6575..6648)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca ttcaggcacc tttcgtcttc acctcgagaa ttgtgagcgg
       61 ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag gacgcactga
      121 ccgaattcat taaagaggag aaaggtacca tatggtgaat gtgaaaccag taacgttata
      181 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      241 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      301 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      361 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
      421 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
      481 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
      541 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
      601 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
      661 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
      721 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
      781 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
      841 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
      901 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
      961 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     1021 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     1081 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     1141 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1201 gcagctggca cgacaggttt cccgactgga aagcgggcag gcagcaaacg acgaaaacta
     1261 cgctttagca gcttaatcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1381 ccctagagcc agtgaggcac ctttcgtctt cacctcgaga attgtgagcg gataacaatt
     1441 gacattgtga gcggataaca agatactgag cacatcagca ggacgcactg accgaattca
     1501 ttaaagagga gaaaggtacc atgtccagat tagataaaag taaagtgatt aacagcgcat
     1561 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     1621 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     1681 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     1741 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     1801 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     1861 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     1921 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     1981 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     2041 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     2101 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc tgcagcaaac gacgaaaact
     2161 acgctttagc agcttaatct agaggcatca aataaaacga aaggctcagt cgaaagactg
     2221 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     2281 gccctagagc cacctaggcg tcgacagatc tcccgggtac cgagctcgaa ttcggctgtt
     2341 ctggtgttgc tagtttgtta tcagaatcgc agatccggct tcaggtttgc cggctgaaag
     2401 cgctatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt
     2461 gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac
     2521 tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta
     2581 ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt
     2641 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
     2701 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
     2761 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
     2821 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
     2881 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
     2941 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
     3001 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
     3061 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
     3121 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
     3181 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
     3241 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
     3301 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
     3361 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
     3421 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     3481 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     3541 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     3601 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     3661 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     3721 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     3781 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     3841 actagtcctt ttcccgggag atctgggtat ctgtaaattc tgctagacct ttgctggaaa
     3901 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     3961 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     4021 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     4081 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     4141 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     4201 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     4261 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     4321 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     4381 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     4441 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     4501 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     4561 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     4621 aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     4681 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     4741 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     4801 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     4861 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     4921 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     4981 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     5041 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     5101 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     5161 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     5221 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     5281 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     5341 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     5401 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     5461 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     5521 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     5581 ctggcaattc cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5641 gtatcacgag gccctttcgt cttcacctcg agagtactgc cacggaggct ctagggcggc
     5701 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     5761 tctttcgact gagcctttcg ttttatttga tgcctctaga tcaagctgct aaagcgtagt
     5821 tttcgtcgtt tgctgcgcca aacgtctctt caggccactg actagcgata actttcccca
     5881 caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     5941 acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     6001 ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     6061 caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     6121 agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     6181 taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     6241 catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     6301 tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca agcaatgcgg
     6361 cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     6421 tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     6481 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     6541 tgctcatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact cga
//
LOCUS       CLT_RFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501247708.281
VERSION     urn.local...1608501247708.281
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             109..1233
                     /label="lacI"
     terminator      complement(1247..1351)
                     /label="T1"
     CDS             complement(1358..2014)
                     /label="tetR"
     misc_feature    complement(2041..2120)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    2120^2121
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      2183..4335
                     /label="SC101* Origin (Approx Position)"
     terminator      4378..4483
                     /label="t0"
     CDS             complement(4509..5303)
                     /label="KnR (neo)"
     misc_feature    5515^5516
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5538..5642)
                     /label="T1"
     CDS             complement(5649..6395)
                     /label="cI"
     misc_feature    complement(6423..6496)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    6501^6502
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatat ggtgaatgtg
      121 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
      181 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
      241 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
      301 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
      361 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
      421 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
      481 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
      541 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
      601 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
      661 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      721 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      781 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      841 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      901 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      961 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1021 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     1081 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     1141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggca
     1201 gcaaacgacg aaaactacgc tttagcagct taagagccag tgaggctcta gggcggcgga
     1261 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     1321 ttcgactgag cctttcgttt tatttgatgc ctctagatta agctgctaaa gcgtagtttt
     1381 cgtcgtttgc tgcagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
     1441 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
     1501 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
     1561 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
     1621 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
     1681 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
     1741 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
     1801 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
     1861 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
     1921 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
     1981 tgttaatcac tttactttta tctaatctgg acatggtacc tttctcctct ttaatgaatt
     2041 cggtcagtgc gtcctgctga tgtgctcagt atcttgttat ccgctcacaa tgtcaattgt
     2101 tatccgctca caattctcga ggtgaagacg aaaggagcca cctaggcgtc gacagatctc
     2161 ccgggtaccg agctcgaatt cggctgttct ggtgttgcta gtttgttatc agaatcgcag
     2221 atccggcttc aggtttgccg gctgaaagcg ctatttcttc cagaattgcc atgatttttt
     2281 ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc tttgattcga taagcagcat
     2341 cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc
     2401 aatttcatgt tctagttgct ttgttttact ggtttcacct gttctattag gtgttacatg
     2461 ctgttcatct gttacattgt cgatctgttc atggtgaaca gctttaaatg caccaaaaac
     2521 tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag
     2581 ttttcccttt gatatctaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc
     2641 ttcactgata gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt
     2701 tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatgct
     2761 tactttgcat gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta
     2821 aagcatcgtg tagtgttttt cttagtccgt tacgtaggta ggaatctgat gtaatggttg
     2881 ttggtatttt gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca
     2941 tttgtctatc tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa
     3001 ccaccaattt catattgctg taagtgttta aatctttact tattggtttc aaaacccatt
     3061 ggttaagcct tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat
     3121 caaggctaat ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc
     3181 actcataaat cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt
     3241 ttatgaattt ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta
     3301 atttttcgct tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca
     3361 aaggattcct gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac
     3421 cataagcatt ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata
     3481 ccgtccgttc tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata
     3541 aaattagctt ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt
     3601 tgaaaacaac taattcagac atacatctca attggtctag gtgattttaa tcactatacc
     3661 aattgagatg ggctagtcaa tgataattac tagtcctttt cccgggagat ctgggtatct
     3721 gtaaattctg ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt
     3781 ccgctagacc tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa
     3841 agaaagaata aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt
     3901 agtcagttcc gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca
     3961 gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata
     4021 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc
     4081 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg
     4141 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt
     4201 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc
     4261 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa
     4321 tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct
     4381 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     4441 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctct cgaaccccag
     4501 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     4561 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     4621 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     4681 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     4741 tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg
     4801 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     4861 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     4921 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     4981 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     5041 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     5101 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca
     5161 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     5221 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     5281 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt
     5341 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
     5401 ttcccaacct taccagaggg cgccccagct ggcaattccg acgtctaaga aaccattatt
     5461 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag
     5521 agtactgcca cggaggctct agggcggcgg atttgtccta ctcaggagag cgttcaccga
     5581 caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg
     5641 cctctagatc aagctgctaa agcgtagttt tcgtcgtttg ctgcgccaaa cgtctcttca
     5701 ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca tgggatcatt
     5761 gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat cagtttcttg
     5821 aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc aacagcctgc
     5881 tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc tgttggtgcg
     5941 gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt tttggttgtg
     6001 cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga gaacatccct
     6061 gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg ctgcatacta
     6121 accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc ttcaacgcta
     6181 actttgagaa tttttgcaag caatgcggcg ttataagcat ttaatgcatt gatgccatta
     6241 aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga ttcctgggat
     6301 aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc gtcctcaagc
     6361 tgctcttgtg ttaatggttt cttttttgtg ctcatggtac ctttctcctc tttaatgaat
     6421 tcggtcagtg cgtcctgctg atgtgctcag tatctctatc actgataggg atgtcaatct
     6481 ctatcactga tagggactcg aggtgaagac gaaaggagcc attcaggcac ctttcgtctt
     6541 cacc
//
LOCUS       CLT_RRR                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187682372.18
VERSION     urn.local...1608187682372.18
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..1263)
                     /label="LacI"
     misc_feature    complement(1292..1371)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    1371^1372
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(1399..1503)
                     /label="T1"
     CDS             complement(1510..2166)
                     /label="TetR"
     misc_feature    complement(2193..2272)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    2272^2273
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      2335..4487
                     /label="SC101* Origin (Approx Position)"
     terminator      4530..4635
                     /label="t0"
     CDS             complement(4661..5455)
                     /label="KnR (neo)"
     misc_feature    5667^5668
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5690..5794)
                     /label="T1"
     CDS             complement(5801..6547)
                     /label="cI"
     misc_feature    complement(6575..6648)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca ttcaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcctgccc
      181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      241 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
      301 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
      361 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
      421 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
      481 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
      541 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
      601 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
      661 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
      721 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
      781 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
      841 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
      901 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
      961 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     1021 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     1081 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     1141 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     1201 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     1261 catatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     1321 tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg aggtgaagac
     1381 gaaaggagcc agtgaggctc tagggcggcg gatttgtcct actcaggaga gcgttcaccg
     1441 acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
     1501 gcctctagat taagctgcta aagcgtagtt ttcgtcgttt gctgcagacc cactttcaca
     1561 tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga aggctggctc
     1621 tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt
     1681 agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc
     1741 taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg
     1801 ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt
     1861 acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc
     1921 gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg
     1981 cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca
     2041 atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc
     2101 gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct
     2161 ggacatggta cctttctcct ctttaatgaa ttcggtcagt gcgtcctgct gatgtgctca
     2221 gtatcttgtt atccgctcac aatgtcaatt gttatccgct cacaattctc gaggtgaaga
     2281 cgaaaggagc cacctaggcg tcgacagatc tcccgggtac cgagctcgaa ttcggctgtt
     2341 ctggtgttgc tagtttgtta tcagaatcgc agatccggct tcaggtttgc cggctgaaag
     2401 cgctatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt
     2461 gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac
     2521 tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta
     2581 ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt
     2641 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
     2701 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
     2761 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
     2821 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
     2881 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
     2941 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
     3001 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
     3061 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
     3121 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
     3181 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
     3241 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
     3301 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
     3361 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
     3421 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     3481 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     3541 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     3601 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     3661 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     3721 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     3781 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     3841 actagtcctt ttcccgggag atctgggtat ctgtaaattc tgctagacct ttgctggaaa
     3901 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     3961 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     4021 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     4081 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     4141 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     4201 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     4261 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     4321 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     4381 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     4441 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     4501 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     4561 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     4621 aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     4681 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     4741 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     4801 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     4861 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     4921 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     4981 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     5041 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     5101 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     5161 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     5221 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     5281 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     5341 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     5401 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     5461 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     5521 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     5581 ctggcaattc cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5641 gtatcacgag gccctttcgt cttcacctcg agagtactgc cacggaggct ctagggcggc
     5701 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     5761 tctttcgact gagcctttcg ttttatttga tgcctctaga tcaagctgct aaagcgtagt
     5821 tttcgtcgtt tgctgcgcca aacgtctctt caggccactg actagcgata actttcccca
     5881 caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     5941 acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     6001 ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     6061 caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     6121 agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     6181 taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     6241 catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     6301 tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca agcaatgcgg
     6361 cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     6421 tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     6481 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     6541 tgctcatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact cga
//
LOCUS       CTL_FFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187712864.97
VERSION     urn.local...1608187712864.97
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1098..1754
                     /label="tetR"
     terminator      1761..1865
                     /label="T1"
     misc_feature    1892^1893
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    1893..1972
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             2001..3125
                     /label="LacI"
     terminator      3132..3236
                     /label="T1"
     rep_origin      3283..5435
                     /label="SC101* Origin (Approx Position)"
     terminator      5478..5583
                     /label="t0"
     CDS             complement(5609..6403)
                     /label="KnR (neo)"
     misc_feature    6615^6616
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccatt caggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccatg tccagattag ataaaagtaa agtgattaac agcgcattag
     1141 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     1201 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     1261 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     1321 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     1381 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     1441 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     1501 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     1561 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     1621 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     1681 gcggattaga aaaacaactt aaatgtgaaa gtgggtctgc agcaaacgac gaaaactacg
     1741 ctttagcagc ttaatctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     1861 ctagagccag tgaggcacct ttcgtcttca cctcgagaat tgtgagcgga taacaattga
     1921 cattgtgagc ggataacaag atactgagca catcagcagg acgcactgac cgaattcatt
     1981 aaagaggaga aaggtaccat atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
     2041 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
     2101 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
     2161 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
     2221 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
     2281 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
     2341 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
     2401 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
     2461 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
     2521 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
     2581 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
     2641 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
     2701 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
     2761 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
     2821 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
     2881 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     2941 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     3001 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3061 gacaggtttc ccgactggaa agcgggcagg cagcaaacga cgaaaactac gctttagcag
     3121 cttaatctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3181 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagagcca
     3241 cctaggcgtc gacagatctc ccgggtaccg agctcgaatt cggctgttct ggtgttgcta
     3301 gtttgttatc agaatcgcag atccggcttc aggtttgccg gctgaaagcg ctatttcttc
     3361 cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt tgtcggcagc
     3421 tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg ttgagctgta
     3481 acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact ggtttcacct
     3541 gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc atggtgaaca
     3601 gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt
     3661 tcatctgtgc atatggacag ttttcccttt gatatctaac ggtgaacagt tgttctactt
     3721 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
     3781 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     3841 cgaaccattg agatcatgct tactttgcat gtcactcaaa aattttgcct caaaactggt
     3901 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tacgtaggta
     3961 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     4021 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     4081 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     4141 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     4201 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     4261 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     4321 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     4381 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     4441 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     4501 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     4561 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     4621 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     4681 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     4741 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac tagtcctttt
     4801 cccgggagat ctgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     4861 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     4921 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     4981 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     5041 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     5101 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     5161 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     5221 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     5281 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg
     5341 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac
     5401 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac aggcttaccc
     5461 gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     5521 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     5581 agcgagctct cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     5641 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     5701 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     5761 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     5821 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     5881 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     5941 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     6001 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     6061 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     6121 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     6181 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     6241 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     6301 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     6361 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     6421 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     6481 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     6541 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     6601 cctttcgtct tcacctcgag agtactgcca cggaggcacc tttcgtcttc acc
//
LOCUS       CTL_FFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187712864.96
VERSION     urn.local...1608187712864.96
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1098..1754
                     /label="TetR"
     terminator      complement(1768..1872)
                     /label="T1"
     CDS             complement(1879..3003)
                     /label="LacI"
     misc_feature    complement(3032..3111)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    3111^3112
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      3174..5326
                     /label="SC101* Origin (Approx Position)"
     terminator      5369..5474
                     /label="t0"
     CDS             complement(5500..6294)
                     /label="KnR (neo)"
     misc_feature    6506^6507
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccatt caggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccatg tccagattag ataaaagtaa agtgattaac agcgcattag
     1141 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     1201 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     1261 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     1321 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     1381 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     1441 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     1501 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     1561 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     1621 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     1681 gcggattaga aaaacaactt aaatgtgaaa gtgggtctgc agcaaacgac gaaaactacg
     1741 ctttagcagc ttaagagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag
     1801 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     1861 ttatttgatg cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcctgccc
     1921 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     1981 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     2041 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     2101 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     2161 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     2221 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     2281 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     2341 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     2401 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     2461 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     2521 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     2581 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     2641 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     2701 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     2761 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     2821 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     2881 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     2941 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     3001 catatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     3061 tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg aggtgaagac
     3121 gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     3181 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     3241 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     3301 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     3361 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     3421 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     3481 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     3541 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     3601 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     3661 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     3721 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     3781 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     3841 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     3901 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     3961 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     4021 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     4081 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     4141 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     4201 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     4261 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     4321 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     4381 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     4441 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     4501 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     4561 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     4621 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     4681 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     4741 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     4801 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     4861 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     4921 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     4981 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     5041 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     5101 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     5161 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     5221 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     5281 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     5341 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     5401 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     5461 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5521 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5581 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5641 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5701 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5761 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5821 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5881 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5941 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     6001 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     6061 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     6121 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     6181 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6241 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     6301 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     6361 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     6421 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6481 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     6541 cacc
//
LOCUS       CTL_FRR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608500861622.189
VERSION     urn.local...1608500861622.189
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      63..2215
                     /label="SC101* Origin (Approx Position)"
     terminator      2258..2363
                     /label="t0"
     CDS             complement(2389..3183)
                     /label="KnR (neo)"
     misc_feature    3395^3396
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    3433^3434
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    3439..3512
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             3540..4286
                     /label="cI"
     terminator      4293..4397
                     /label="T1"
     CDS             complement(4411..5067)
                     /label="tetR"
     misc_feature    complement(5094..5173)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5173^5174
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5201..5305)
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="lacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca cctaggcgtc gacagatctc ccgggtaccg agctcgaatt
       61 cggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg
      121 gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg
      181 gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta
      241 tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct
      301 ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt
      361 cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat
      421 ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac
      481 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
      541 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
      601 ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa
      661 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
      721 cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
      781 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
      841 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
      901 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
      961 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     1021 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     1081 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     1141 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     1201 gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca
     1261 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     1321 atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta
     1381 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
     1441 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
     1501 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
     1561 tgataattac tagtcctttt cccgggagat ctgggtatct gtaaattctg ctagaccttt
     1621 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
     1681 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
     1741 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac
     1801 aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc
     1861 ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     1921 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     1981 agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc
     2041 cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg
     2101 tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc
     2161 acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg
     2221 tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa
     2281 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2341 gatctatcaa caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc
     2401 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2461 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2521 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2581 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2641 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2701 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2761 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2821 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2881 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2941 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3001 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3061 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3121 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3181 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     3241 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3301 cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa
     3361 aaataggcgt atcacgaggc cctttcgtct tcacctcgag agtactgcca cggaggcacc
     3421 tttcgtcttc acctcgagtc cctatcagtg atagagattg acatccctat cagtgataga
     3481 gatactgagc acatcagcag gacgcactga ccgaattcat taaagaggag aaaggtacca
     3541 tgagcacaaa aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag
     3601 caatttatga aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga
     3661 tggggatggg gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt
     3721 ataacgccgc attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa
     3781 tcgccagaga aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg
     3841 agtatgagta ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa
     3901 cctttaccaa aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg
     3961 cattctggct tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct
     4021 ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct
     4081 gcatagccag acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc
     4141 aggtgttttt acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt
     4201 ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gcagcaaacg
     4261 acgaaaacta cgctttagca gcttgatcta gaggcatcaa ataaaacgaa aggctcagtc
     4321 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     4381 aaatccgccg ccctagagcc attcaggctc ttaagctgct aaagcgtagt tttcgtcgtt
     4441 tgctgcagac ccactttcac atttaagttg tttttctaat ccgcatatga tcaattcaag
     4501 gccgaataag aaggctggct ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa
     4561 taatggcggc atactatcag tagtaggtgt ttccctttct tctttagcga cttgatgctc
     4621 ttgatcttcc aatacgcaac ctaaagtaaa atgccccaca gcgctgagtg catataatgc
     4681 attctctagt gaaaaacctt gttggcataa aaaggctaat tgattttcga gagtttcata
     4741 ctgtttttct gtaggccgtg tacctaaatg tacttttgct ccatcgcgat gacttagtaa
     4801 agcacatcta aaacttttag cgttattacg taaaaaatct tgccagcttt ccccttctaa
     4861 agggcaaaag tgagtatggt gcctatctaa catctcaatg gctaaggcgt cgagcaaagc
     4921 ccgcttattt tttacatgcc aatacaatgt aggctgctct acacctagct tctgggcgag
     4981 tttacgggtt gttaaacctt cgattccgac ctcattaagc agctctaatg cgctgttaat
     5041 cactttactt ttatctaatc tggacatggt acctttctcc tctttaatga attcggtcag
     5101 tgcgtcctgc tgatgtgctc agtatcttgt tatccgctca caatgtcaat tgttatccgc
     5161 tcacaattct cgaggtgaag acgaaaggag ccagtgaggc tctagggcgg cggatttgtc
     5221 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     5281 tgagcctttc gttttatttg atgcctctag attaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       CTL_RFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501309305.299
VERSION     urn.local...1608501309305.299
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      63..2215
                     /label="SC101* Origin (Approx Position)"
     terminator      2258..2363
                     /label="t0"
     CDS             complement(2389..3183)
                     /label="KnR (neo)"
     misc_feature    3395^3396
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(3418..3522)
                     /label="T1"
     CDS             complement(3529..4275)
                     /label="cI"
     misc_feature    complement(4303..4376)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    4381^4382
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4424^4425
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4425..4504
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4531..5187
                     /label="tetR"
     terminator      complement(5201..5305)
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="lacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca cctaggcgtc gacagatctc ccgggtaccg agctcgaatt
       61 cggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg
      121 gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg
      181 gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta
      241 tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct
      301 ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt
      361 cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat
      421 ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac
      481 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
      541 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
      601 ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa
      661 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
      721 cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
      781 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
      841 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
      901 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
      961 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     1021 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     1081 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     1141 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     1201 gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca
     1261 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     1321 atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta
     1381 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
     1441 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
     1501 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
     1561 tgataattac tagtcctttt cccgggagat ctgggtatct gtaaattctg ctagaccttt
     1621 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
     1681 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
     1741 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac
     1801 aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc
     1861 ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     1921 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     1981 agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc
     2041 cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg
     2101 tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc
     2161 acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg
     2221 tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa
     2281 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2341 gatctatcaa caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc
     2401 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2461 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2521 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2581 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2641 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2701 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2761 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2821 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2881 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2941 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3001 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3061 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3121 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3181 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     3241 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3301 cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa
     3361 aaataggcgt atcacgaggc cctttcgtct tcacctcgag agtactgcca cggaggctct
     3421 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3481 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctctagatc aagctgctaa
     3541 agcgtagttt tcgtcgtttg ctgcgccaaa cgtctcttca ggccactgac tagcgataac
     3601 tttccccaca acggaacaac tctcattgca tgggatcatt gggtactgtg ggtttagtgg
     3661 ttgtaaaaac acctgaccgc tatccctgat cagtttcttg aaggtaaact catcaccccc
     3721 aagtctggct atgcagaaat cacctggctc aacagcctgc tcagggtcaa cgagaattaa
     3781 cattccgtca ggaaagcttg gcttggagcc tgttggtgcg gtcatggaat taccttcaac
     3841 ctcaagccag aatgcagaat cactggcttt tttggttgtg cttacccatc tctccgcatc
     3901 acctttggta aaggttctaa gctcaggtga gaacatccct gcctgaacat gagaaaaaac
     3961 agggtactca tactcacttc taagtgacgg ctgcatacta accgcttcat acatctcgta
     4021 gatttctctg gcgattgaag ggctaaattc ttcaacgcta actttgagaa tttttgcaag
     4081 caatgcggcg ttataagcat ttaatgcatt gatgccatta aataaagcac caacgcctga
     4141 ctgccccatc cccatcttgt ctgcgacaga ttcctgggat aagccaagtt catttttctt
     4201 tttttcataa attgctttaa ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt
     4261 cttttttgtg ctcatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg
     4321 atgtgctcag tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg
     4381 aggtgaagac gaaaggagcc attcaggcac ctttcgtctt cacctcgaga attgtgagcg
     4441 gataacaatt gacattgtga gcggataaca agatactgag cacatcagca ggacgcactg
     4501 accgaattca ttaaagagga gaaaggtacc atgtccagat tagataaaag taaagtgatt
     4561 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     4621 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     4681 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     4741 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     4801 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     4861 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     4921 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     4981 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     5041 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     5101 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc tgcagcaaac
     5161 gacgaaaact acgctttagc agcttaagag ccagtgaggc tctagggcgg cggatttgtc
     5221 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     5281 tgagcctttc gttttatttg atgcctctag attaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       CTL_RRR                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187712834.90
VERSION     urn.local...1608187712834.90
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..795)
                     /label="TetR"
     misc_feature    complement(822..901)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    901^902
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(929..1033)
                     /label="T1"
     CDS             complement(1040..2164)
                     /label="LacI"
     misc_feature    complement(2193..2272)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    2272^2273
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      2335..4487
                     /label="SC101* Origin (Approx Position)"
     terminator      4530..4635
                     /label="t0"
     CDS             complement(4661..5455)
                     /label="KnR (neo)"
     misc_feature    5667^5668
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5690..5794)
                     /label="T1"
     CDS             complement(5801..6547)
                     /label="cI"
     misc_feature    complement(6575..6648)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca ttcaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcagaccc
      181 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      241 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      301 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      361 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      421 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      481 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      541 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      601 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      661 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      721 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      781 atctaatctg gacatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg
      841 atgtgctcag tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg
      901 aggtgaagac gaaaggagcc agtgaggctc tagggcggcg gatttgtcct actcaggaga
      961 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     1021 tttatttgat gcctctagat taagctgcta aagcgtagtt ttcgtcgttt gctgcctgcc
     1081 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     1141 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca
     1201 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg
     1261 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc
     1321 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact
     1381 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg
     1441 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt
     1501 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag
     1561 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt
     1621 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa
     1681 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg
     1741 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga
     1801 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta
     1861 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa
     1921 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt
     1981 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt
     2041 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg
     2101 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca
     2161 ccatatggta cctttctcct ctttaatgaa ttcggtcagt gcgtcctgct gatgtgctca
     2221 gtatcttgtt atccgctcac aatgtcaatt gttatccgct cacaattctc gaggtgaaga
     2281 cgaaaggagc cacctaggcg tcgacagatc tcccgggtac cgagctcgaa ttcggctgtt
     2341 ctggtgttgc tagtttgtta tcagaatcgc agatccggct tcaggtttgc cggctgaaag
     2401 cgctatttct tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt
     2461 gttgtcggca gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac
     2521 tgttgagctg taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta
     2581 ctggtttcac ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt
     2641 tcatggtgaa cagctttaaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt
     2701 tttacaccgt tttcatctgt gcatatggac agttttccct ttgatatcta acggtgaaca
     2761 gttgttctac ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga
     2821 acctcagatc cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt
     2881 gagccatgag aacgaaccat tgagatcatg cttactttgc atgtcactca aaaattttgc
     2941 ctcaaaactg gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc
     3001 gttacgtagg taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat
     3061 ctggttgttc tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat
     3121 caacgtatca gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt
     3181 taaatcttta cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt
     3241 attttcaagc attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg
     3301 agttttcttt tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt
     3361 ttcaaaagac ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata
     3421 aggcaatatc tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt
     3481 tgtccactgg aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt
     3541 catcagctct ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat
     3601 catctgagcg tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc
     3661 aatcgtgggg ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa
     3721 gtcatagcga ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct
     3781 caattggtct aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt
     3841 actagtcctt ttcccgggag atctgggtat ctgtaaattc tgctagacct ttgctggaaa
     3901 acttgtaaat tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt
     3961 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
     4021 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
     4081 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
     4141 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     4201 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     4261 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     4321 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     4381 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     4441 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     4501 acaggcttac ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg
     4561 cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc
     4621 aacaggagtc caagcgagct ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa
     4681 ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc
     4741 ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac gctatgtcct
     4801 gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag cggccatttt
     4861 ccaccatgat attcggcaag caggcatcgc catgggtcac gacgagatcc tcgccgtcgg
     4921 gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga tgctcttcgt
     4981 ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc tcgatgcgat
     5041 gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc cgccgcattg
     5101 catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg agatcctgcc
     5161 ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg tcgagcacag
     5221 ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg tcctgcagtt
     5281 cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca
     5341 gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca tagccgaata
     5401 gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa
     5461 acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag atccttggcg
     5521 gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag
     5581 ctggcaattc cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5641 gtatcacgag gccctttcgt cttcacctcg agagtactgc cacggaggct ctagggcggc
     5701 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     5761 tctttcgact gagcctttcg ttttatttga tgcctctaga tcaagctgct aaagcgtagt
     5821 tttcgtcgtt tgctgcgcca aacgtctctt caggccactg actagcgata actttcccca
     5881 caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     5941 acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     6001 ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     6061 caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     6121 agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     6181 taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     6241 catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     6301 tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca agcaatgcgg
     6361 cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     6421 tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     6481 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     6541 tgctcatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact cga
//
LOCUS       LCT_FFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187695784.61
VERSION     urn.local...1608187695784.61
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1098..1754
                     /label="TetR"
     terminator      1761..1865
                     /label="T1"
     rep_origin      1912..4064
                     /label="SC101* Origin (Approx Position)"
     terminator      4107..4212
                     /label="t0"
     CDS             complement(4238..5032)
                     /label="KnR (neo)"
     misc_feature    5244^5245
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5282^5283
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5283..5362
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             5391..6515
                     /label="LacI"
     terminator      6522..6626
                     /label="T1"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccagt gaggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccatg tccagattag ataaaagtaa agtgattaac agcgcattag
     1141 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     1201 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     1261 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     1321 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     1381 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     1441 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     1501 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     1561 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     1621 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     1681 gcggattaga aaaacaactt aaatgtgaaa gtgggtctgc agcaaacgac gaaaactacg
     1741 ctttagcagc ttaatctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     1861 ctagagccac ctaggcgtcg acagatctcc cgggtaccga gctcgaattc ggctgttctg
     1921 gtgttgctag tttgttatca gaatcgcaga tccggcttca ggtttgccgg ctgaaagcgc
     1981 tatttcttcc agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt
     2041 gtcggcagct ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt
     2101 tgagctgtaa caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg
     2161 gtttcacctg ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca
     2221 tggtgaacag ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
     2281 acaccgtttt catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt
     2341 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
     2401 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
     2461 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
     2521 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
     2581 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
     2641 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
     2701 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
     2761 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
     2821 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
     2881 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
     2941 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
     3001 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     3061 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     3121 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     3181 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     3241 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     3301 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     3361 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     3421 agtccttttc ccgggagatc tgggtatctg taaattctgc tagacctttg ctggaaaact
     3481 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     3541 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     3601 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     3661 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     3721 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     3781 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     3841 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     3901 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     3961 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     4021 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     4081 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     4141 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     4201 aggagtccaa gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     4261 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     4321 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     4381 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     4441 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     4501 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     4561 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     4621 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     4681 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     4741 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     4801 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
     4861 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     4921 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     4981 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     5041 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     5101 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     5161 gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5221 tcacgaggcc ctttcgtctt cacctcgaga gtactgccac ggaggcacct ttcgtcttca
     5281 cctcgagaat tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca
     5341 catcagcagg acgcactgac cgaattcatt aaagaggaga aaggtaccat atggtgaatg
     5401 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     5461 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     5521 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     5581 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     5641 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     5701 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     5761 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     5821 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     5881 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     5941 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     6001 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     6061 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     6121 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     6181 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     6241 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     6301 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     6361 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     6421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg
     6481 cagcaaacga cgaaaactac gctttagcag cttaatctag aggcatcaaa taaaacgaaa
     6541 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     6601 gagtaggaca aatccgccgc cctagagcca ttcaggcacc tttcgtcttc acc
//
LOCUS       LCT_FRF                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608500512705.117
VERSION     urn.local...1608500512705.117
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    43^44
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    44..123
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             150..806
                     /label="tetR"
     terminator      813..917
                     /label="T1"
     rep_origin      964..3116
                     /label="SC101* Origin (Approx Position)"
     terminator      3159..3264
                     /label="t0"
     CDS             complement(3290..4084)
                     /label="KnR (neo)"
     misc_feature    4296^4297
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4334^4335
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4335..4414
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4443..5567
                     /label="LacI"
     terminator      5574..5678
                     /label="T1"
     CDS             complement(5692..6438)
                     /label="cI"
     misc_feature    complement(6466..6539)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc acctcgagaa ttgtgagcgg
       61 ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag gacgcactga
      121 ccgaattcat taaagaggag aaaggtacca tgtccagatt agataaaagt aaagtgatta
      181 acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg
      241 cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt
      301 tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag
      361 aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac
      421 taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg
      481 aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat
      541 tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc
      601 atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac
      661 gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg
      721 aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtct gcagcaaacg
      781 acgaaaacta cgctttagca gcttaatcta gaggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      901 aaatccgccg ccctagagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat
      961 tcggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc
     1021 ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact
     1081 ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct
     1141 atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc
     1201 tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg
     1261 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
     1321 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa
     1381 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     1441 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     1501 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     1561 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     1621 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     1681 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     1741 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     1801 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     1861 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     1921 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     1981 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     2041 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     2101 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     2161 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     2221 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     2281 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     2341 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     2401 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     2461 atgataatta ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt
     2521 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     2581 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     2641 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     2701 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     2761 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     2821 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     2881 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     2941 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     3001 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     3061 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     3121 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     3181 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     3241 ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact
     3301 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     3361 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     3421 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     3481 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     3541 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     3601 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     3661 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     3721 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     3781 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     3841 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     3901 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     3961 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     4021 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     4081 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
     4141 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     4201 gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata
     4261 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac
     4321 ctttcgtctt cacctcgaga attgtgagcg gataacaatt gacattgtga gcggataaca
     4381 agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc
     4441 atatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     4501 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     4561 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     4621 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     4681 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     4741 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     4801 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     4861 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     4921 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     4981 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     5041 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     5101 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     5161 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     5221 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     5281 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     5341 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     5401 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     5461 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5521 aaagcgggca ggcagcaaac gacgaaaact acgctttagc agcttaatct agaggcatca
     5581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     5641 gaacgctctc ctgagtagga caaatccgcc gccctagagc cattcaggct cttaagctgc
     5701 taaagcgtag ttttcgtcgt ttgctgcgcc aaacgtctct tcaggccact gactagcgat
     5761 aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact gtgggtttag
     5821 tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa actcatcacc
     5881 cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt caacgagaat
     5941 taacattccg tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg aattaccttc
     6001 aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgcttaccc atctctccgc
     6061 atcacctttg gtaaaggttc taagctcagg tgagaacatc cctgcctgaa catgagaaaa
     6121 aacagggtac tcatactcac ttctaagtga cggctgcata ctaaccgctt catacatctc
     6181 gtagatttct ctggcgattg aagggctaaa ttcttcaacg ctaactttga gaatttttgc
     6241 aagcaatgcg gcgttataag catttaatgc attgatgcca ttaaataaag caccaacgcc
     6301 tgactgcccc atccccatct tgtctgcgac agattcctgg gataagccaa gttcattttt
     6361 ctttttttca taaattgctt taaggcgacg tgcgtcctca agctgctctt gtgttaatgg
     6421 tttctttttt gtgctcatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggac
     6541 tcga
//
LOCUS       LCT_FRR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608500956933.207
VERSION     urn.local...1608500956933.207
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..795)
                     /label="tetR"
     misc_feature    complement(822..901)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    901^902
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      964..3116
                     /label="SC101* Origin (Approx Position)"
     terminator      3159..3264
                     /label="t0"
     CDS             complement(3290..4084)
                     /label="KnR (neo)"
     misc_feature    4296^4297
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4334^4335
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4335..4414
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4443..5567
                     /label="lacI"
     terminator      5574..5678
                     /label="T1"
     CDS             complement(5692..6438)
                     /label="cI"
     misc_feature    complement(6466..6539)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcagaccc
      181 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      241 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      301 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      361 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      421 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      481 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      541 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      601 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      661 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      721 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      781 atctaatctg gacatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg
      841 atgtgctcag tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg
      901 aggtgaagac gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat
      961 tcggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc
     1021 ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact
     1081 ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct
     1141 atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc
     1201 tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg
     1261 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
     1321 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa
     1381 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     1441 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     1501 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     1561 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     1621 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     1681 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     1741 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     1801 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     1861 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     1921 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     1981 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     2041 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     2101 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     2161 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     2221 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     2281 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     2341 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     2401 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     2461 atgataatta ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt
     2521 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     2581 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     2641 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     2701 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     2761 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     2821 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     2881 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     2941 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     3001 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     3061 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     3121 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     3181 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     3241 ggatctatca acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact
     3301 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     3361 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     3421 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     3481 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     3541 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     3601 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     3661 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     3721 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     3781 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     3841 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     3901 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     3961 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     4021 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     4081 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
     4141 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
     4201 gcgccccagc tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata
     4261 aaaataggcg tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac
     4321 ctttcgtctt cacctcgaga attgtgagcg gataacaatt gacattgtga gcggataaca
     4381 agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc
     4441 atatggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt
     4501 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa
     4561 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg
     4621 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt
     4681 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt
     4741 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc
     4801 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg
     4861 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca
     4921 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat
     4981 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc
     5041 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg
     5101 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca
     5161 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca
     5221 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg
     5281 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg
     5341 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc
     5401 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg
     5461 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5521 aaagcgggca ggcagcaaac gacgaaaact acgctttagc agcttaatct agaggcatca
     5581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     5641 gaacgctctc ctgagtagga caaatccgcc gccctagagc cattcaggct cttaagctgc
     5701 taaagcgtag ttttcgtcgt ttgctgcgcc aaacgtctct tcaggccact gactagcgat
     5761 aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact gtgggtttag
     5821 tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa actcatcacc
     5881 cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt caacgagaat
     5941 taacattccg tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg aattaccttc
     6001 aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgcttaccc atctctccgc
     6061 atcacctttg gtaaaggttc taagctcagg tgagaacatc cctgcctgaa catgagaaaa
     6121 aacagggtac tcatactcac ttctaagtga cggctgcata ctaaccgctt catacatctc
     6181 gtagatttct ctggcgattg aagggctaaa ttcttcaacg ctaactttga gaatttttgc
     6241 aagcaatgcg gcgttataag catttaatgc attgatgcca ttaaataaag caccaacgcc
     6301 tgactgcccc atccccatct tgtctgcgac agattcctgg gataagccaa gttcattttt
     6361 ctttttttca taaattgctt taaggcgacg tgcgtcctca agctgctctt gtgttaatgg
     6421 tttctttttt gtgctcatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggac
     6541 tcga
//
LOCUS       LCT_RFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187695754.57
VERSION     urn.local...1608187695754.57
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1098..1754
                     /label="TetR"
     terminator      1761..1865
                     /label="T1"
     rep_origin      1912..4064
                     /label="SC101* Origin (Approx Position)"
     terminator      4107..4212
                     /label="t0"
     CDS             complement(4238..5032)
                     /label="KnR (neo)"
     misc_feature    5244^5245
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5267..5371)
                     /label="T1"
     CDS             complement(5378..6502)
                     /label="LacI"
     misc_feature    complement(6531..6610)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    6610^6611
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccagt gaggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccatg tccagattag ataaaagtaa agtgattaac agcgcattag
     1141 agctgcttaa tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag
     1201 gtgtagagca gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct
     1261 tagccattga gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct
     1321 ggcaagattt tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg
     1381 atggagcaaa agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa
     1441 atcaattagc ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca
     1501 gcgctgtggg gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta
     1561 aagaagaaag ggaaacacct actactgata gtatgccgcc attattacga caagctatcg
     1621 aattatttga tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat
     1681 gcggattaga aaaacaactt aaatgtgaaa gtgggtctgc agcaaacgac gaaaactacg
     1741 ctttagcagc ttaatctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     1861 ctagagccac ctaggcgtcg acagatctcc cgggtaccga gctcgaattc ggctgttctg
     1921 gtgttgctag tttgttatca gaatcgcaga tccggcttca ggtttgccgg ctgaaagcgc
     1981 tatttcttcc agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt
     2041 gtcggcagct ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt
     2101 tgagctgtaa caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg
     2161 gtttcacctg ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca
     2221 tggtgaacag ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
     2281 acaccgtttt catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt
     2341 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
     2401 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
     2461 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
     2521 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
     2581 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
     2641 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
     2701 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
     2761 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
     2821 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
     2881 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
     2941 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
     3001 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     3061 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     3121 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     3181 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     3241 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     3301 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     3361 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
     3421 agtccttttc ccgggagatc tgggtatctg taaattctgc tagacctttg ctggaaaact
     3481 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     3541 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     3601 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     3661 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     3721 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     3781 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     3841 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     3901 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     3961 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     4021 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     4081 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     4141 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     4201 aggagtccaa gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc
     4261 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     4321 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     4381 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     4441 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     4501 tgcgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     4561 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     4621 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     4681 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     4741 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     4801 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat
     4861 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     4921 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     4981 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     5041 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     5101 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     5161 gcaattccga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     5221 tcacgaggcc ctttcgtctt cacctcgaga gtactgccac ggaggctcta gggcggcgga
     5281 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     5341 ttcgactgag cctttcgttt tatttgatgc ctctagatta agctgctaaa gcgtagtttt
     5401 cgtcgtttgc tgcctgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     5521 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     5581 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     5641 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     5701 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     5761 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     5821 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     5881 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     5941 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     6001 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     6061 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     6121 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     6181 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     6241 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     6301 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     6361 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     6421 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     6481 ttactggttt cacattcacc atatggtacc tttctcctct ttaatgaatt cggtcagtgc
     6541 gtcctgctga tgtgctcagt atcttgttat ccgctcacaa tgtcaattgt tatccgctca
     6601 caattctcga ggtgaagacg aaaggagcca ttcaggcacc tttcgtcttc acc
//
LOCUS       LCT_RFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501369579.318
VERSION     urn.local...1608501369579.318
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    43^44
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    49..122
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             150..896
                     /label="cI"
     terminator      complement(910..1014)
                     /label="T1"
     CDS             complement(1021..1677)
                     /label="tetR"
     misc_feature    complement(1704..1783)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    1783^1784
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      1846..3998
                     /label="SC101* Origin (Approx Position)"
     terminator      4041..4146
                     /label="t0"
     CDS             complement(4172..4966)
                     /label="KnR (neo)"
     misc_feature    5178^5179
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5201..5305)
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="lacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca ttcaggcacc tttcgtcttc acctcgagtc cctatcagtg
       61 atagagattg acatccctat cagtgataga gatactgagc acatcagcag gacgcactga
      121 ccgaattcat taaagaggag aaaggtacca tgagcacaaa aaagaaacca ttaacacaag
      181 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg
      241 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt
      301 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca
      361 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag
      421 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
      481 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg
      541 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca
      601 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc
      661 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta
      721 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt
      781 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt
      841 ggcctgaaga gacgtttggc gcagcaaacg acgaaaacta cgctttagca gcttaagagc
      901 cagtgaggct ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      961 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctctaga
     1021 ttaagctgct aaagcgtagt tttcgtcgtt tgctgcagac ccactttcac atttaagttg
     1081 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
     1141 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
     1201 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
     1261 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
     1321 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
     1381 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
     1441 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
     1501 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
     1561 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
     1621 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tggacatggt
     1681 acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc agtatcttgt
     1741 tatccgctca caatgtcaat tgttatccgc tcacaattct cgaggtgaag acgaaaggag
     1801 ccacctaggc gtcgacagat ctcccgggta ccgagctcga attcggctgt tctggtgttg
     1861 ctagtttgtt atcagaatcg cagatccggc ttcaggtttg ccggctgaaa gcgctatttc
     1921 ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc
     1981 agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct
     2041 gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca
     2101 cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga
     2161 acagctttaa atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg
     2221 ttttcatctg tgcatatgga cagttttccc tttgatatct aacggtgaac agttgttcta
     2281 cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag aacctcagat
     2341 ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg tgagccatga
     2401 gaacgaacca ttgagatcat gcttactttg catgtcactc aaaaattttg cctcaaaact
     2461 ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc cgttacgtag
     2521 gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta tctggttgtt
     2581 ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa tcaacgtatc
     2641 agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt
     2701 acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt tattttcaag
     2761 cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt gagttttctt
     2821 ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt tttcaaaaga
     2881 cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat aaggcaatat
     2941 ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt ttgtccactg
     3001 gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg tcatcagctc
     3061 tctggttgct ttagctaata caccataagc attttcccta ctgatgttca tcatctgagc
     3121 gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt caatcgtggg
     3181 gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta agtcatagcg
     3241 actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc tcaattggtc
     3301 taggtgattt taatcactat accaattgag atgggctagt caatgataat tactagtcct
     3361 tttcccggga gatctgggta tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa
     3421 ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg tttatattca
     3481 agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa tagatcccag
     3541 ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac
     3601 gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta gcaccctcgc
     3661 aagctcgggc aaatcgctga atattccttt tgtctccgac catcaggcac ctgagtcgct
     3721 gtctttttcg tgacattcag ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat
     3781 ggcactacag gcgcctttta tggattcatg caaggaaact acccataata caagaaaagc
     3841 ccgtcacggg cttctcaggg cgttttatgg cgggtctgct atgtggtgct atctgacttt
     3901 ttgctgttca gcagttcctg ccctctgatt ttccagtctg accacttcgg attatcccgt
     3961 gacaggtcat tcagactggc taatgcaccc agtaaggcag cggtatcatc aacaggctta
     4021 cccgtcttac tgtccctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     4081 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     4141 ccaagcgagc tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     4201 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     4261 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     4321 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     4381 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     4441 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     4501 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     4561 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     4621 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     4681 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     4741 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     4801 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     4861 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     4921 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     4981 atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
     5041 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
     5101 ccgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     5161 ggccctttcg tcttcacctc gagagtactg ccacggaggc tctagggcgg cggatttgtc
     5221 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     5281 tgagcctttc gttttatttg atgcctctag attaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       LCT_RRF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187695744.55
VERSION     urn.local...1608187695744.55
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..885)
                     /label="cI"
     misc_feature    complement(913..986)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    1034^1035
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    1035..1114
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1141..1797
                     /label="TetR"
     terminator      1804..1908
                     /label="T1"
     rep_origin      1955..4107
                     /label="SC101* Origin (Approx Position)"
     terminator      4150..4255
                     /label="t0"
     CDS             complement(4281..5075)
                     /label="KnR (neo)"
     misc_feature    5287^5288
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5310..5414)
                     /label="T1"
     CDS             complement(5421..6545)
                     /label="LacI"
     misc_feature    complement(6574..6653)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca ttcaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatc aagctgctaa agcgtagttt tcgtcgtttg ctgcgccaaa
      181 cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca
      241 tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat
      301 cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc
      361 aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc
      421 tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt
      481 tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga
      541 gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg
      601 ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc
      661 ttcaacgcta actttgagaa tttttgcaag caatgcggcg ttataagcat ttaatgcatt
      721 gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga
      781 ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc
      841 gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatggtac ctttctcctc
      901 tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag tatctctatc actgataggg
      961 atgtcaatct ctatcactga tagggactcg aggtgaagac gaaaggagcc agtgaggcac
     1021 ctttcgtctt cacctcgaga attgtgagcg gataacaatt gacattgtga gcggataaca
     1081 agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc
     1141 atgtccagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc
     1201 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca
     1261 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta
     1321 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt
     1381 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat
     1441 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta
     1501 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt
     1561 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca
     1621 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa
     1681 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa
     1741 cttaaatgtg aaagtgggtc tgcagcaaac gacgaaaact acgctttagc agcttaatct
     1801 agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1861 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagagc cacctaggcg
     1921 tcgacagatc tcccgggtac cgagctcgaa ttcggctgtt ctggtgttgc tagtttgtta
     1981 tcagaatcgc agatccggct tcaggtttgc cggctgaaag cgctatttct tccagaattg
     2041 ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc
     2101 gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg
     2161 tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt
     2221 aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttaaa
     2281 tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt
     2341 gcatatggac agttttccct ttgatatcta acggtgaaca gttgttctac ttttgtttgt
     2401 tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt
     2461 tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat
     2521 tgagatcatg cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa
     2581 tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg
     2641 atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg
     2701 ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc
     2761 ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt
     2821 tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga
     2881 acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt
     2941 cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt
     3001 ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa
     3061 aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa
     3121 agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt
     3181 tagctaatac accataagca ttttccctac tgatgttcat catctgagcg tattggttat
     3241 aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg
     3301 ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta
     3361 gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt
     3421 aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt ttcccgggag
     3481 atctgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac
     3541 cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa
     3601 tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata
     3661 actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc
     3721 ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca
     3781 aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt
     3841 gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg
     3901 cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc
     3961 ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag
     4021 cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt
     4081 cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact
     4141 gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     4201 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     4261 ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     4321 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     4381 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     4441 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     4501 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     4561 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     4621 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     4681 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     4741 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     4801 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     4861 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
     4921 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     4981 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     5041 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     5101 tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     5161 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cgacgtctaa
     5221 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     5281 cttcacctcg agagtactgc cacggaggct ctagggcggc ggatttgtcc tactcaggag
     5341 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     5401 ttttatttga tgcctctaga ttaagctgct aaagcgtagt tttcgtcgtt tgctgcctgc
     5461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5521 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     5581 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5641 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5701 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5761 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5821 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5881 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     5941 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     6001 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     6061 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     6121 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     6181 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     6241 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     6301 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     6361 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     6421 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     6481 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     6541 accatatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatcttgt tatccgctca caatgtcaat tgttatccgc tcacaattct cga
//
LOCUS       LCT_RRR                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187695744.54
VERSION     urn.local...1608187695744.54
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..885)
                     /label="cI"
     misc_feature    complement(913..986)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(1019..1123)
                     /label="T1"
     CDS             complement(1130..1786)
                     /label="TetR"
     misc_feature    complement(1813..1892)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    1892^1893
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      1955..4107
                     /label="SC101* Origin (Approx Position)"
     terminator      4150..4255
                     /label="t0"
     CDS             complement(4281..5075)
                     /label="KnR (neo)"
     misc_feature    5287^5288
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5310..5414)
                     /label="T1"
     CDS             complement(5421..6545)
                     /label="LacI"
     misc_feature    complement(6574..6653)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca ttcaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatc aagctgctaa agcgtagttt tcgtcgtttg ctgcgccaaa
      181 cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca
      241 tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat
      301 cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc
      361 aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc
      421 tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt
      481 tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga
      541 gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg
      601 ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc
      661 ttcaacgcta actttgagaa tttttgcaag caatgcggcg ttataagcat ttaatgcatt
      721 gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga
      781 ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc
      841 gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatggtac ctttctcctc
      901 tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag tatctctatc actgataggg
      961 atgtcaatct ctatcactga tagggactcg aggtgaagac gaaaggagcc agtgaggctc
     1021 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     1081 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctctagat taagctgcta
     1141 aagcgtagtt ttcgtcgttt gctgcagacc cactttcaca tttaagttgt ttttctaatc
     1201 cgcatatgat caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata
     1261 attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt
     1321 ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag
     1381 cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt
     1441 gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc
     1501 catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt
     1561 gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg
     1621 ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta
     1681 cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca
     1741 gctctaatgc gctgttaatc actttacttt tatctaatct ggacatggta cctttctcct
     1801 ctttaatgaa ttcggtcagt gcgtcctgct gatgtgctca gtatcttgtt atccgctcac
     1861 aatgtcaatt gttatccgct cacaattctc gaggtgaaga cgaaaggagc cacctaggcg
     1921 tcgacagatc tcccgggtac cgagctcgaa ttcggctgtt ctggtgttgc tagtttgtta
     1981 tcagaatcgc agatccggct tcaggtttgc cggctgaaag cgctatttct tccagaattg
     2041 ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca gctttgattc
     2101 gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg taacaagttg
     2161 tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac ctgttctatt
     2221 aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa cagctttaaa
     2281 tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt
     2341 gcatatggac agttttccct ttgatatcta acggtgaaca gttgttctac ttttgtttgt
     2401 tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt
     2461 tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat
     2521 tgagatcatg cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa
     2581 tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg
     2641 atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg
     2701 ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc
     2761 ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt
     2821 tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga
     2881 acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt
     2941 cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt
     3001 ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa
     3061 aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa
     3121 agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt
     3181 tagctaatac accataagca ttttccctac tgatgttcat catctgagcg tattggttat
     3241 aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg
     3301 ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta
     3361 gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt
     3421 aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt ttcccgggag
     3481 atctgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac
     3541 cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa
     3601 tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata
     3661 actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc
     3721 ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca
     3781 aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt
     3841 gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg
     3901 cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc
     3961 ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag
     4021 cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt
     4081 cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact
     4141 gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     4201 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     4261 ctcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     4321 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     4381 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     4441 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     4501 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     4561 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     4621 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     4681 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     4741 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     4801 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     4861 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
     4921 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     4981 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     5041 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     5101 tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     5161 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cgacgtctaa
     5221 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     5281 cttcacctcg agagtactgc cacggaggct ctagggcggc ggatttgtcc tactcaggag
     5341 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     5401 ttttatttga tgcctctaga ttaagctgct aaagcgtagt tttcgtcgtt tgctgcctgc
     5461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5521 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     5581 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5641 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5701 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5761 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5821 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5881 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     5941 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     6001 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     6061 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     6121 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     6181 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     6241 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     6301 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     6361 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     6421 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     6481 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     6541 accatatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatcttgt tatccgctca caatgtcaat tgttatccgc tcacaattct cga
//
LOCUS       LTC_FFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187724440.133
VERSION     urn.local...1608187724440.133
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     rep_origin      1011..3163
                     /label="SC101* Origin (Approx Position)"
     terminator      3206..3311
                     /label="t0"
     CDS             complement(3337..4131)
                     /label="KnR (neo)"
     misc_feature    4343^4344
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4381^4382
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4382..4461
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4490..5614
                     /label="LacI"
     terminator      5621..5725
                     /label="T1"
     misc_feature    5752^5753
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5753..5832
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             5859..6515
                     /label="TetR"
     terminator      6522..6626
                     /label="T1"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccacc taggcgtcga cagatctccc gggtaccgag ctcgaattcg gctgttctgg
     1021 tgttgctagt ttgttatcag aatcgcagat ccggcttcag gtttgccggc tgaaagcgct
     1081 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     1141 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     1201 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     1261 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     1321 ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     1381 caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg tgaacagttg
     1441 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     1501 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     1561 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     1621 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     1681 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     1741 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     1801 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     1861 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     1921 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     1981 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     2041 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     2101 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     2161 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     2221 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     2281 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     2341 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     2401 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     2461 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     2521 gtccttttcc cgggagatct gggtatctgt aaattctgct agacctttgc tggaaaactt
     2581 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     2641 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     2701 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     2761 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     2821 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     2881 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     2941 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     3001 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     3061 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     3121 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     3181 gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     3241 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3301 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     3361 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3421 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3481 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3541 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3601 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3661 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3721 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3781 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3841 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3901 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3961 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4021 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4081 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4141 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     4201 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4261 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4321 cacgaggccc tttcgtcttc acctcgagag tactgccacg gaggcacctt tcgtcttcac
     4381 ctcgagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
     4441 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccata tggtgaatgt
     4501 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     4561 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     4621 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     4681 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     4741 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     4801 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     4861 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     4921 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     4981 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     5041 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
     5101 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
     5161 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
     5221 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
     5281 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
     5341 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     5401 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
     5461 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
     5521 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc
     5581 agcaaacgac gaaaactacg ctttagcagc ttaatctaga ggcatcaaat aaaacgaaag
     5641 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     5701 agtaggacaa atccgccgcc ctagagccat tcaggcacct ttcgtcttca cctcgagaat
     5761 tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca catcagcagg
     5821 acgcactgac cgaattcatt aaagaggaga aaggtaccat gtccagatta gataaaagta
     5881 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
     5941 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
     6001 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
     6061 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
     6121 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
     6181 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
     6241 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
     6301 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     6361 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     6421 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctg
     6481 cagcaaacga cgaaaactac gctttagcag cttaatctag aggcatcaaa taaaacgaaa
     6541 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     6601 gagtaggaca aatccgccgc cctagagcca gtgaggcacc tttcgtcttc acc
//
LOCUS       LTC_FFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187724420.132
VERSION     urn.local...1608187724420.132
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             109..1233
                     /label="LacI"
     terminator      1240..1344
                     /label="T1"
     misc_feature    1371^1372
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    1372..1451
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1478..2134
                     /label="TetR"
     terminator      complement(2148..2252)
                     /label="T1"
     CDS             complement(2259..3005)
                     /label="cI"
     misc_feature    complement(3033..3106)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    3111^3112
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      3174..5326
                     /label="SC101* Origin (Approx Position)"
     terminator      5369..5474
                     /label="t0"
     CDS             complement(5500..6294)
                     /label="KnR (neo)"
     misc_feature    6506^6507
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatat ggtgaatgtg
      121 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
      181 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
      241 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
      301 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
      361 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
      421 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
      481 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
      541 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
      601 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
      661 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      721 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      781 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      841 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      901 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      961 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1021 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     1081 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     1141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggca
     1201 gcaaacgacg aaaactacgc tttagcagct taatctagag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1321 gtaggacaaa tccgccgccc tagagccatt caggcacctt tcgtcttcac ctcgagaatt
     1381 gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac atcagcagga
     1441 cgcactgacc gaattcatta aagaggagaa aggtaccatg tccagattag ataaaagtaa
     1501 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
     1561 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
     1621 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
     1681 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
     1741 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
     1801 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
     1861 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
     1921 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
     1981 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
     2041 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtctgc
     2101 agcaaacgac gaaaactacg ctttagcagc ttaagagcca gtgaggctct agggcggcgg
     2161 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     2221 tttcgactga gcctttcgtt ttatttgatg cctctagatc aagctgctaa agcgtagttt
     2281 tcgtcgtttg ctgcgccaaa cgtctcttca ggccactgac tagcgataac tttccccaca
     2341 acggaacaac tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac
     2401 acctgaccgc tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct
     2461 atgcagaaat cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca
     2521 ggaaagcttg gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag
     2581 aatgcagaat cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta
     2641 aaggttctaa gctcaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca
     2701 tactcacttc taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg
     2761 gcgattgaag ggctaaattc ttcaacgcta actttgagaa tttttgcaag caatgcggcg
     2821 ttataagcat ttaatgcatt gatgccatta aataaagcac caacgcctga ctgccccatc
     2881 cccatcttgt ctgcgacaga ttcctgggat aagccaagtt catttttctt tttttcataa
     2941 attgctttaa ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg
     3001 ctcatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     3061 tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg aggtgaagac
     3121 gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     3181 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     3241 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     3301 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     3361 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     3421 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     3481 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     3541 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     3601 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     3661 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     3721 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     3781 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     3841 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     3901 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     3961 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     4021 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     4081 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     4141 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     4201 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     4261 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     4321 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     4381 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     4441 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     4501 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     4561 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     4621 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     4681 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     4741 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     4801 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     4861 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     4921 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     4981 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     5041 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     5101 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     5161 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     5221 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     5281 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     5341 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     5401 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     5461 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5521 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5581 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5641 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5701 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5761 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5821 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5881 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5941 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     6001 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     6061 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     6121 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     6181 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6241 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     6301 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     6361 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     6421 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6481 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     6541 cacc
//
LOCUS       LTC_FRF                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187724420.131
VERSION     urn.local...1608187724420.131
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             109..1233
                     /label="LacI"
     terminator      1240..1344
                     /label="T1"
     CDS             complement(1358..2014)
                     /label="TetR"
     misc_feature    complement(2041..2120)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    2120^2121
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    2163^2164
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    2169..2242
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             2270..3016
                     /label="cI"
     terminator      3023..3127
                     /label="T1"
     rep_origin      3174..5326
                     /label="SC101* Origin (Approx Position)"
     terminator      5369..5474
                     /label="t0"
     CDS             complement(5500..6294)
                     /label="KnR (neo)"
     misc_feature    6506^6507
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatat ggtgaatgtg
      121 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
      181 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
      241 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
      301 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
      361 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
      421 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
      481 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
      541 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
      601 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
      661 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      721 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      781 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      841 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      901 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      961 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1021 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     1081 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     1141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggca
     1201 gcaaacgacg aaaactacgc tttagcagct taatctagag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1321 gtaggacaaa tccgccgccc tagagccatt caggctctta agctgctaaa gcgtagtttt
     1381 cgtcgtttgc tgcagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
     1441 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
     1501 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
     1561 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
     1621 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
     1681 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
     1741 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
     1801 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
     1861 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
     1921 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
     1981 tgttaatcac tttactttta tctaatctgg acatggtacc tttctcctct ttaatgaatt
     2041 cggtcagtgc gtcctgctga tgtgctcagt atcttgttat ccgctcacaa tgtcaattgt
     2101 tatccgctca caattctcga ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc
     2161 acctcgagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
     2221 acatcagcag gacgcactga ccgaattcat taaagaggag aaaggtacca tgagcacaaa
     2281 aaagaaacca ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga
     2341 aaaaaagaaa aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg
     2401 gcagtcaggc gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc
     2461 attgcttgca aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga
     2521 aatctacgag atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta
     2581 ccctgttttt tctcatgttc aggcagggat gttctcacct gagcttagaa cctttaccaa
     2641 aggtgatgcg gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct
     2701 tgaggttgaa ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg
     2761 aatgttaatt ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag
     2821 acttgggggt gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt
     2881 acaaccacta aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg
     2941 gaaagttatc gctagtcagt ggcctgaaga gacgtttggc gcagcaaacg acgaaaacta
     3001 cgctttagca gcttgatcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     3061 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     3121 ccctagagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     3181 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     3241 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     3301 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     3361 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     3421 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     3481 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     3541 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     3601 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     3661 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     3721 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     3781 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     3841 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     3901 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     3961 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     4021 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     4081 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     4141 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     4201 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     4261 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     4321 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     4381 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     4441 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     4501 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     4561 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     4621 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     4681 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     4741 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     4801 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     4861 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     4921 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     4981 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     5041 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     5101 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     5161 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     5221 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     5281 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     5341 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     5401 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     5461 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5521 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5581 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5641 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5701 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5761 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5821 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5881 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5941 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     6001 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     6061 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     6121 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     6181 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6241 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     6301 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     6361 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     6421 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6481 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     6541 cacc
//
LOCUS       LTC_FRR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501033339.227
VERSION     urn.local...1608501033339.227
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             109..1233
                     /label="lacI"
     terminator      1240..1344
                     /label="T1"
     CDS             complement(1358..2014)
                     /label="tetR"
     misc_feature    complement(2041..2120)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    2120^2121
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(2148..2252)
                     /label="T1"
     CDS             complement(2259..3005)
                     /label="cI"
     misc_feature    complement(3033..3106)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    3111^3112
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      3174..5326
                     /label="SC101* Origin (Approx Position)"
     terminator      5369..5474
                     /label="t0"
     CDS             complement(5500..6294)
                     /label="KnR (neo)"
     misc_feature    6506^6507
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatat ggtgaatgtg
      121 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
      181 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
      241 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
      301 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
      361 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
      421 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
      481 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
      541 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
      601 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
      661 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      721 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      781 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      841 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      901 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      961 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1021 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     1081 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     1141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggca
     1201 gcaaacgacg aaaactacgc tttagcagct taatctagag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1321 gtaggacaaa tccgccgccc tagagccatt caggctctta agctgctaaa gcgtagtttt
     1381 cgtcgtttgc tgcagaccca ctttcacatt taagttgttt ttctaatccg catatgatca
     1441 attcaaggcc gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt
     1501 gtcgtaataa tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt
     1561 gatgctcttg atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat
     1621 ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag
     1681 tttcatactg tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac
     1741 ttagtaaagc acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc
     1801 cttctaaagg gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga
     1861 gcaaagcccg cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct
     1921 gggcgagttt acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc
     1981 tgttaatcac tttactttta tctaatctgg acatggtacc tttctcctct ttaatgaatt
     2041 cggtcagtgc gtcctgctga tgtgctcagt atcttgttat ccgctcacaa tgtcaattgt
     2101 tatccgctca caattctcga ggtgaagacg aaaggagcca gtgaggctct agggcggcgg
     2161 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc
     2221 tttcgactga gcctttcgtt ttatttgatg cctctagatc aagctgctaa agcgtagttt
     2281 tcgtcgtttg ctgcgccaaa cgtctcttca ggccactgac tagcgataac tttccccaca
     2341 acggaacaac tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac
     2401 acctgaccgc tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct
     2461 atgcagaaat cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca
     2521 ggaaagcttg gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag
     2581 aatgcagaat cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta
     2641 aaggttctaa gctcaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca
     2701 tactcacttc taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg
     2761 gcgattgaag ggctaaattc ttcaacgcta actttgagaa tttttgcaag caatgcggcg
     2821 ttataagcat ttaatgcatt gatgccatta aataaagcac caacgcctga ctgccccatc
     2881 cccatcttgt ctgcgacaga ttcctgggat aagccaagtt catttttctt tttttcataa
     2941 attgctttaa ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg
     3001 ctcatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     3061 tatctctatc actgataggg atgtcaatct ctatcactga tagggactcg aggtgaagac
     3121 gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     3181 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     3241 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     3301 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     3361 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     3421 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     3481 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     3541 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     3601 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     3661 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     3721 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     3781 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     3841 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     3901 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     3961 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     4021 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     4081 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     4141 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     4201 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     4261 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     4321 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     4381 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     4441 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     4501 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     4561 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     4621 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     4681 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     4741 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     4801 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     4861 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     4921 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     4981 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     5041 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     5101 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     5161 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     5221 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     5281 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     5341 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     5401 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     5461 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     5521 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     5581 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     5641 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     5701 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     5761 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     5821 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     5881 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     5941 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     6001 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     6061 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     6121 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     6181 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     6241 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     6301 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     6361 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     6421 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     6481 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     6541 cacc
//
LOCUS       LTC_RRF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187724390.127
VERSION     urn.local...1608187724390.127
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     rep_origin      1011..3163
                     /label="SC101* Origin (Approx Position)"
     terminator      3206..3311
                     /label="t0"
     CDS             complement(3337..4131)
                     /label="KnR (neo)"
     misc_feature    4343^4344
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4366..4470)
                     /label="T1"
     CDS             complement(4477..5601)
                     /label="LacI"
     misc_feature    complement(5630..5709)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5709^5710
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5737..5841)
                     /label="T1"
     CDS             complement(5848..6504)
                     /label="TetR"
     misc_feature    complement(6531..6610)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    6610^6611
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccacc taggcgtcga cagatctccc gggtaccgag ctcgaattcg gctgttctgg
     1021 tgttgctagt ttgttatcag aatcgcagat ccggcttcag gtttgccggc tgaaagcgct
     1081 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     1141 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     1201 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     1261 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     1321 ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     1381 caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg tgaacagttg
     1441 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     1501 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     1561 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     1621 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     1681 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     1741 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     1801 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     1861 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     1921 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     1981 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     2041 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     2101 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     2161 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     2221 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     2281 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     2341 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     2401 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     2461 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     2521 gtccttttcc cgggagatct gggtatctgt aaattctgct agacctttgc tggaaaactt
     2581 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     2641 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     2701 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     2761 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     2821 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     2881 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     2941 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     3001 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     3061 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     3121 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     3181 gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     3241 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3301 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     3361 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3421 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3481 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3541 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3601 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3661 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3721 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3781 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3841 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3901 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3961 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4021 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4081 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4141 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     4201 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4261 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4321 cacgaggccc tttcgtcttc acctcgagag tactgccacg gaggctctag ggcggcggat
     4381 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4441 tcgactgagc ctttcgtttt atttgatgcc tctagattaa gctgctaaag cgtagttttc
     4501 gtcgtttgct gcctgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4561 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     4621 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     4681 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     4741 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
     4801 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     4861 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     4921 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     4981 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     5041 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     5101 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     5161 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     5221 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     5281 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     5341 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     5401 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     5461 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     5521 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     5581 tactggtttc acattcacca tatggtacct ttctcctctt taatgaattc ggtcagtgcg
     5641 tcctgctgat gtgctcagta tcttgttatc cgctcacaat gtcaattgtt atccgctcac
     5701 aattctcgag gtgaagacga aaggagccat tcaggctcta gggcggcgga tttgtcctac
     5761 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     5821 cctttcgttt tatttgatgc ctctagatta agctgctaaa gcgtagtttt cgtcgtttgc
     5881 tgcagaccca ctttcacatt taagttgttt ttctaatccg catatgatca attcaaggcc
     5941 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
     6001 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
     6061 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
     6121 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
     6181 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
     6241 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
     6301 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
     6361 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
     6421 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
     6481 tttactttta tctaatctgg acatggtacc tttctcctct ttaatgaatt cggtcagtgc
     6541 gtcctgctga tgtgctcagt atcttgttat ccgctcacaa tgtcaattgt tatccgctca
     6601 caattctcga ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc acc
//
LOCUS       LTC_RRR                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187724380.126
VERSION     urn.local...1608187724380.126
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      63..2215
                     /label="SC101* Origin (Approx Position)"
     terminator      2258..2363
                     /label="t0"
     CDS             complement(2389..3183)
                     /label="KnR (neo)"
     misc_feature    3395^3396
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(3418..3522)
                     /label="T1"
     CDS             complement(3529..4653)
                     /label="LacI"
     misc_feature    complement(4682..4761)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    4761^4762
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4789..4893)
                     /label="T1"
     CDS             complement(4900..5556)
                     /label="TetR"
     misc_feature    complement(5583..5662)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5662^5663
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5690..5794)
                     /label="T1"
     CDS             complement(5801..6547)
                     /label="cI"
     misc_feature    complement(6575..6648)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca cctaggcgtc gacagatctc ccgggtaccg agctcgaatt
       61 cggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg
      121 gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg
      181 gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta
      241 tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct
      301 ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt
      361 cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat
      421 ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac
      481 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
      541 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
      601 ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa
      661 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
      721 cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
      781 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
      841 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
      901 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
      961 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     1021 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     1081 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     1141 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     1201 gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca
     1261 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     1321 atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta
     1381 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
     1441 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
     1501 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
     1561 tgataattac tagtcctttt cccgggagat ctgggtatct gtaaattctg ctagaccttt
     1621 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
     1681 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
     1741 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac
     1801 aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc
     1861 ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     1921 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     1981 agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc
     2041 cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg
     2101 tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc
     2161 acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg
     2221 tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa
     2281 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2341 gatctatcaa caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc
     2401 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2461 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2521 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2581 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2641 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2701 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2761 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2821 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2881 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2941 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3001 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3061 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3121 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3181 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     3241 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3301 cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa
     3361 aaataggcgt atcacgaggc cctttcgtct tcacctcgag agtactgcca cggaggctct
     3421 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3481 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctctagatt aagctgctaa
     3541 agcgtagttt tcgtcgtttg ctgcctgccc gctttccagt cgggaaacct gtcgtgccag
     3601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     3661 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     3721 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     3781 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     3841 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     3901 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     3961 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     4021 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     4081 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     4141 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     4201 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     4261 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     4321 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     4381 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     4441 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     4501 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     4561 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     4621 atcgtataac gttactggtt tcacattcac catatggtac ctttctcctc tttaatgaat
     4681 tcggtcagtg cgtcctgctg atgtgctcag tatcttgtta tccgctcaca atgtcaattg
     4741 ttatccgctc acaattctcg aggtgaagac gaaaggagcc attcaggctc tagggcggcg
     4801 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4861 ctttcgactg agcctttcgt tttatttgat gcctctagat taagctgcta aagcgtagtt
     4921 ttcgtcgttt gctgcagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
     4981 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
     5041 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
     5101 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     5161 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     5221 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     5281 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     5341 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     5401 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     5461 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     5521 gctgttaatc actttacttt tatctaatct ggacatggta cctttctcct ctttaatgaa
     5581 ttcggtcagt gcgtcctgct gatgtgctca gtatcttgtt atccgctcac aatgtcaatt
     5641 gttatccgct cacaattctc gaggtgaaga cgaaaggagc cagtgaggct ctagggcggc
     5701 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     5761 tctttcgact gagcctttcg ttttatttga tgcctctaga tcaagctgct aaagcgtagt
     5821 tttcgtcgtt tgctgcgcca aacgtctctt caggccactg actagcgata actttcccca
     5881 caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     5941 acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     6001 ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     6061 caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     6121 agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     6181 taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     6241 catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     6301 tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca agcaatgcgg
     6361 cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     6421 tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     6481 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     6541 tgctcatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact cga
//
LOCUS       TCL_FFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187736441.169
VERSION     urn.local...1608187736441.169
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    992..1071
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             1100..2224
                     /label="LacI"
     terminator      2231..2335
                     /label="T1"
     rep_origin      2382..4534
                     /label="SC101* Origin (Approx Position)"
     terminator      4577..4682
                     /label="t0"
     CDS             complement(4708..5502)
                     /label="KnR (neo)"
     misc_feature    5714^5715
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5752^5753
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5753..5832
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             5859..6515
                     /label="TetR"
     terminator      6522..6626
                     /label="T1"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccagt gaggcacctt tcgtcttcac ctcgagaatt gtgagcggat aacaattgac
     1021 attgtgagcg gataacaaga tactgagcac atcagcagga cgcactgacc gaattcatta
     1081 aagaggagaa aggtaccata tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     1141 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     1201 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     1261 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     1321 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     1381 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     1441 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     1501 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     1561 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     1621 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     1681 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     1741 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     1801 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     1861 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     1921 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     1981 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     2041 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     2101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2161 acaggtttcc cgactggaaa gcgggcaggc agcaaacgac gaaaactacg ctttagcagc
     2221 ttaatctaga ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2281 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc ctagagccac
     2341 ctaggcgtcg acagatctcc cgggtaccga gctcgaattc ggctgttctg gtgttgctag
     2401 tttgttatca gaatcgcaga tccggcttca ggtttgccgg ctgaaagcgc tatttcttcc
     2461 agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct
     2521 ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa
     2581 caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg
     2641 ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag
     2701 ctttaaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     2761 catctgtgca tatggacagt tttccctttg atatctaacg gtgaacagtt gttctacttt
     2821 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     2881 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     2941 gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     3001 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag
     3061 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     3121 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     3181 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     3241 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     3301 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     3361 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     3421 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     3481 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     3541 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     3601 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     3661 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     3721 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     3781 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     3841 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     3901 ccgggagatc tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     3961 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     4021 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     4081 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     4141 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     4201 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     4261 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     4321 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     4381 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     4441 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     4501 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
     4561 tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     4621 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     4681 gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     4741 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     4801 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     4861 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     4921 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
     4981 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     5041 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     5101 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     5161 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     5221 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     5281 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
     5341 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     5401 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     5461 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
     5521 tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
     5581 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga
     5641 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     5701 ctttcgtctt cacctcgaga gtactgccac ggaggcacct ttcgtcttca cctcgagaat
     5761 tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca catcagcagg
     5821 acgcactgac cgaattcatt aaagaggaga aaggtaccat gtccagatta gataaaagta
     5881 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc
     5941 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata
     6001 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt
     6061 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat
     6121 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa
     6181 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag
     6241 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag
     6301 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc
     6361 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat
     6421 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtctg
     6481 cagcaaacga cgaaaactac gctttagcag cttaatctag aggcatcaaa taaaacgaaa
     6541 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     6601 gagtaggaca aatccgccgc cctagagcca ttcaggcacc tttcgtcttc acc
//
LOCUS       TCL_FRF                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608500625954.135
VERSION     urn.local...1608500625954.135
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    43^44
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    44..123
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             152..1276
                     /label="lacI"
     terminator      1283..1387
                     /label="T1"
     rep_origin      1434..3586
                     /label="SC101* Origin (Approx Position)"
     terminator      3629..3734
                     /label="t0"
     CDS             complement(3760..4554)
                     /label="KnR (neo)"
     misc_feature    4766^4767
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4804^4805
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4805..4884
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4911..5567
                     /label="tetR"
     terminator      5574..5678
                     /label="T1"
     CDS             complement(5692..6438)
                     /label="cI"
     misc_feature    complement(6466..6539)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc acctcgagaa ttgtgagcgg
       61 ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag gacgcactga
      121 ccgaattcat taaagaggag aaaggtacca tatggtgaat gtgaaaccag taacgttata
      181 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      241 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      301 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      361 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
      421 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
      481 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
      541 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
      601 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
      661 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
      721 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
      781 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
      841 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
      901 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
      961 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     1021 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     1081 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     1141 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1201 gcagctggca cgacaggttt cccgactgga aagcgggcag gcagcaaacg acgaaaacta
     1261 cgctttagca gcttaatcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1381 ccctagagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     1441 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     1501 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     1561 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     1621 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     1681 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     1741 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     1801 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     1861 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     1921 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     1981 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     2041 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     2101 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     2161 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     2221 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     2281 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     2341 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     2401 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     2461 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     2521 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     2581 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     2641 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     2701 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     2761 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     2821 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     2881 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     2941 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     3001 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     3061 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     3121 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     3181 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     3241 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     3301 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     3361 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     3421 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     3481 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     3541 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     3601 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     3661 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     3721 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     3781 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3841 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3901 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3961 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     4021 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     4081 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     4141 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     4201 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     4261 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     4321 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     4381 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     4441 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     4501 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     4561 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     4621 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     4681 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4741 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     4801 cacctcgaga attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag
     4861 cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc atgtccagat
     4921 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     4981 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     5041 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     5101 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     5161 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     5221 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     5281 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     5341 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     5401 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     5461 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     5521 aaagtgggtc tgcagcaaac gacgaaaact acgctttagc agcttaatct agaggcatca
     5581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     5641 gaacgctctc ctgagtagga caaatccgcc gccctagagc cattcaggct cttaagctgc
     5701 taaagcgtag ttttcgtcgt ttgctgcgcc aaacgtctct tcaggccact gactagcgat
     5761 aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact gtgggtttag
     5821 tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa actcatcacc
     5881 cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt caacgagaat
     5941 taacattccg tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg aattaccttc
     6001 aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgcttaccc atctctccgc
     6061 atcacctttg gtaaaggttc taagctcagg tgagaacatc cctgcctgaa catgagaaaa
     6121 aacagggtac tcatactcac ttctaagtga cggctgcata ctaaccgctt catacatctc
     6181 gtagatttct ctggcgattg aagggctaaa ttcttcaacg ctaactttga gaatttttgc
     6241 aagcaatgcg gcgttataag catttaatgc attgatgcca ttaaataaag caccaacgcc
     6301 tgactgcccc atccccatct tgtctgcgac agattcctgg gataagccaa gttcattttt
     6361 ctttttttca taaattgctt taaggcgacg tgcgtcctca agctgctctt gtgttaatgg
     6421 tttctttttt gtgctcatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggac
     6541 tcga
//
LOCUS       TCL_FRR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501086316.245
VERSION     urn.local...1608501086316.245
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..1263)
                     /label="lacI"
     misc_feature    complement(1292..1371)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    1371^1372
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      1434..3586
                     /label="SC101* Origin (Approx Position)"
     terminator      3629..3734
                     /label="t0"
     CDS             complement(3760..4554)
                     /label="KnR (neo)"
     misc_feature    4766^4767
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4804^4805
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4805..4884
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4911..5567
                     /label="tetR"
     terminator      5574..5678
                     /label="T1"
     CDS             complement(5692..6438)
                     /label="cI"
     misc_feature    complement(6466..6539)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatt aagctgctaa agcgtagttt tcgtcgtttg ctgcctgccc
      181 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      241 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
      301 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
      361 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
      421 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
      481 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
      541 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
      601 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
      661 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
      721 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
      781 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
      841 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
      901 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
      961 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     1021 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     1081 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     1141 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     1201 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     1261 catatggtac ctttctcctc tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag
     1321 tatcttgtta tccgctcaca atgtcaattg ttatccgctc acaattctcg aggtgaagac
     1381 gaaaggagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     1441 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     1501 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     1561 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     1621 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     1681 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     1741 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     1801 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     1861 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     1921 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     1981 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     2041 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     2101 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     2161 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     2221 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     2281 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     2341 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     2401 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     2461 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     2521 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     2581 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     2641 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     2701 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     2761 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     2821 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     2881 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     2941 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     3001 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     3061 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     3121 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     3181 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     3241 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     3301 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     3361 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     3421 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     3481 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     3541 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     3601 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     3661 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     3721 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     3781 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3841 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3901 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3961 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     4021 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     4081 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     4141 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     4201 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     4261 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     4321 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     4381 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     4441 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     4501 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     4561 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     4621 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     4681 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4741 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggcac ctttcgtctt
     4801 cacctcgaga attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag
     4861 cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc atgtccagat
     4921 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     4981 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     5041 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     5101 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     5161 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     5221 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     5281 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     5341 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     5401 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     5461 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     5521 aaagtgggtc tgcagcaaac gacgaaaact acgctttagc agcttaatct agaggcatca
     5581 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     5641 gaacgctctc ctgagtagga caaatccgcc gccctagagc cattcaggct cttaagctgc
     5701 taaagcgtag ttttcgtcgt ttgctgcgcc aaacgtctct tcaggccact gactagcgat
     5761 aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact gtgggtttag
     5821 tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa actcatcacc
     5881 cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt caacgagaat
     5941 taacattccg tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg aattaccttc
     6001 aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgcttaccc atctctccgc
     6061 atcacctttg gtaaaggttc taagctcagg tgagaacatc cctgcctgaa catgagaaaa
     6121 aacagggtac tcatactcac ttctaagtga cggctgcata ctaaccgctt catacatctc
     6181 gtagatttct ctggcgattg aagggctaaa ttcttcaacg ctaactttga gaatttttgc
     6241 aagcaatgcg gcgttataag catttaatgc attgatgcca ttaaataaag caccaacgcc
     6301 tgactgcccc atccccatct tgtctgcgac agattcctgg gataagccaa gttcattttt
     6361 ctttttttca taaattgctt taaggcgacg tgcgtcctca agctgctctt gtgttaatgg
     6421 tttctttttt gtgctcatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggac
     6541 tcga
//
LOCUS       TCL_RFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187736431.165
VERSION     urn.local...1608187736431.165
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             109..1233
                     /label="LacI"
     terminator      1240..1344
                     /label="T1"
     rep_origin      1391..3543
                     /label="SC101* Origin (Approx Position)"
     terminator      3586..3691
                     /label="t0"
     CDS             complement(3717..4511)
                     /label="KnR (neo)"
     misc_feature    4723^4724
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4746..4850)
                     /label="T1"
     CDS             complement(4857..5513)
                     /label="TetR"
     misc_feature    complement(5540..5619)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5619^5620
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5662^5663
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5668..5741
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             5769..6515
                     /label="cI"
     terminator      6522..6626
                     /label="T1"
ORIGIN      
        1 tcgagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatat ggtgaatgtg
      121 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
      181 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
      241 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
      301 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
      361 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
      421 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
      481 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
      541 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
      601 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
      661 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      721 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      781 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      841 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      901 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      961 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1021 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     1081 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     1141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggca
     1201 gcaaacgacg aaaactacgc tttagcagct taatctagag gcatcaaata aaacgaaagg
     1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1321 gtaggacaaa tccgccgccc tagagccacc taggcgtcga cagatctccc gggtaccgag
     1381 ctcgaattcg gctgttctgg tgttgctagt ttgttatcag aatcgcagat ccggcttcag
     1441 gtttgccggc tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg
     1501 cgtcactggc tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag
     1561 gctgtctatg tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc
     1621 tagttgcttt gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt
     1681 tacattgtcg atctgttcat ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc
     1741 tgatgtatct atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga
     1801 tatctaacgg tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga
     1861 tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg
     1921 ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt
     1981 cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta
     2041 gtgtttttct tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt
     2101 caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta
     2161 gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca
     2221 tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt
     2281 taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct
     2341 ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc
     2401 tcatagagta tttgttttca aaagacttaa catgttccag attatatttt atgaattttt
     2461 ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg
     2521 agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga
     2581 tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt
     2641 ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt
     2701 tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg
     2761 tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta
     2821 attcagacat acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg
     2881 ctagtcaatg ataattacta gtccttttcc cgggagatct gggtatctgt aaattctgct
     2941 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt
     3001 tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa
     3061 aaaagataaa aagaatagat cccagccctg tgtataactc actactttag tcagttccgc
     3121 agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc
     3181 ctaaaggctt aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct
     3241 ccgaccatca ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg
     3301 gctctggcag tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg
     3361 aaactaccca taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt
     3421 ctgctatgtg gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca
     3481 gtctgaccac ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa
     3541 ggcagcggta tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc
     3601 aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt
     3661 cattactgga tctatcaaca ggagtccaag cgagctctcg aaccccagag tcccgctcag
     3721 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     3781 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     3841 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     3901 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     3961 agatcctcgc cgtcgggcat gcgcgccttg agcctggcga acagttcggc tggcgcgagc
     4021 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     4081 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     4141 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     4201 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     4261 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     4321 gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     4381 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     4441 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     4501 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cagatcttga tcccctgcgc
     4561 catcagatcc ttggcggcaa gaaagccatc cagtttactt tgcagggctt cccaacctta
     4621 ccagagggcg ccccagctgg caattccgac gtctaagaaa ccattattat catgacatta
     4681 acctataaaa ataggcgtat cacgaggccc tttcgtcttc acctcgagag tactgccacg
     4741 gaggctctag ggcggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
     4801 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tctagattaa
     4861 gctgctaaag cgtagttttc gtcgtttgct gcagacccac tttcacattt aagttgtttt
     4921 tctaatccgc atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga
     4981 tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc
     5041 ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc
     5101 cccacagcgc tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag
     5161 gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact
     5221 tttgctccat cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa
     5281 aaatcttgcc agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc
     5341 tcaatggcta aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc
     5401 tgctctacac ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca
     5461 ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctgga catggtacct
     5521 ttctcctctt taatgaattc ggtcagtgcg tcctgctgat gtgctcagta tcttgttatc
     5581 cgctcacaat gtcaattgtt atccgctcac aattctcgag gtgaagacga aaggagccat
     5641 tcaggcacct ttcgtcttca cctcgagtcc ctatcagtga tagagattga catccctatc
     5701 agtgatagag atactgagca catcagcagg acgcactgac cgaattcatt aaagaggaga
     5761 aaggtaccat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc
     5821 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg
     5881 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat
     5941 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta
     6001 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac
     6061 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg
     6121 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca
     6181 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca
     6241 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag
     6301 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg
     6361 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg
     6421 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg
     6481 cagcaaacga cgaaaactac gctttagcag cttgatctag aggcatcaaa taaaacgaaa
     6541 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     6601 gagtaggaca aatccgccgc cctagagcca gtgaggcacc tttcgtcttc acc
//
LOCUS       TCL_RFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501424866.336
VERSION     urn.local...1608501424866.336
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      63..2215
                     /label="SC101* Origin (Approx Position)"
     terminator      2258..2363
                     /label="t0"
     CDS             complement(2389..3183)
                     /label="KnR (neo)"
     misc_feature    3395^3396
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(3418..3522)
                     /label="T1"
     CDS             complement(3529..4185)
                     /label="tetR"
     misc_feature    complement(4212..4291)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    4291^4292
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4334^4335
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4340..4413
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             4441..5187
                     /label="cI"
     terminator      complement(5201..5305)
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="lacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca cctaggcgtc gacagatctc ccgggtaccg agctcgaatt
       61 cggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg
      121 gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg
      181 gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta
      241 tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct
      301 ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt
      361 cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat
      421 ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac
      481 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
      541 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
      601 ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa
      661 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
      721 cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
      781 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
      841 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
      901 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
      961 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     1021 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     1081 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     1141 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     1201 gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca
     1261 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     1321 atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta
     1381 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
     1441 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
     1501 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
     1561 tgataattac tagtcctttt cccgggagat ctgggtatct gtaaattctg ctagaccttt
     1621 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
     1681 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
     1741 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac
     1801 aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc
     1861 ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     1921 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     1981 agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc
     2041 cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg
     2101 tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc
     2161 acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg
     2221 tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa
     2281 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2341 gatctatcaa caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc
     2401 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2461 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2521 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2581 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2641 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2701 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2761 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2821 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2881 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2941 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3001 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3061 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3121 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3181 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     3241 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3301 cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa
     3361 aaataggcgt atcacgaggc cctttcgtct tcacctcgag agtactgcca cggaggctct
     3421 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3481 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctctagatt aagctgctaa
     3541 agcgtagttt tcgtcgtttg ctgcagaccc actttcacat ttaagttgtt tttctaatcc
     3601 gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa
     3661 ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc
     3721 tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc
     3781 gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg
     3841 attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc
     3901 atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg
     3961 ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc
     4021 taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac
     4081 acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag
     4141 ctctaatgcg ctgttaatca ctttactttt atctaatctg gacatggtac ctttctcctc
     4201 tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag tatcttgtta tccgctcaca
     4261 atgtcaattg ttatccgctc acaattctcg aggtgaagac gaaaggagcc attcaggcac
     4321 ctttcgtctt cacctcgagt ccctatcagt gatagagatt gacatcccta tcagtgatag
     4381 agatactgag cacatcagca ggacgcactg accgaattca ttaaagagga gaaaggtacc
     4441 atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa
     4501 gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag
     4561 atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct
     4621 tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt tagcccttca
     4681 atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt
     4741 gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc tgagcttaga
     4801 acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct
     4861 gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc
     4921 tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc
     4981 tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt
     5041 caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt
     5101 tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg cgcagcaaac
     5161 gacgaaaact acgctttagc agcttaagag ccagtgaggc tctagggcgg cggatttgtc
     5221 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     5281 tgagcctttc gttttatttg atgcctctag attaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       TCL_RRF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187736421.163
VERSION     urn.local...1608187736421.163
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    43^44
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    44..123
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             152..1276
                     /label="LacI"
     terminator      1283..1387
                     /label="T1"
     rep_origin      1434..3586
                     /label="SC101* Origin (Approx Position)"
     terminator      3629..3734
                     /label="t0"
     CDS             complement(3760..4554)
                     /label="KnR (neo)"
     misc_feature    4766^4767
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4789..4893)
                     /label="T1"
     CDS             complement(4900..5556)
                     /label="TetR"
     misc_feature    complement(5583..5662)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5662^5663
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5690..5794)
                     /label="T1"
     CDS             complement(5801..6547)
                     /label="cI"
     misc_feature    complement(6575..6648)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc acctcgagaa ttgtgagcgg
       61 ataacaattg acattgtgag cggataacaa gatactgagc acatcagcag gacgcactga
      121 ccgaattcat taaagaggag aaaggtacca tatggtgaat gtgaaaccag taacgttata
      181 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
      241 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
      301 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
      361 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
      421 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
      481 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
      541 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
      601 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
      661 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
      721 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
      781 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
      841 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
      901 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
      961 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac
     1021 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     1081 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     1141 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1201 gcagctggca cgacaggttt cccgactgga aagcgggcag gcagcaaacg acgaaaacta
     1261 cgctttagca gcttaatcta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1321 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg
     1381 ccctagagcc acctaggcgt cgacagatct cccgggtacc gagctcgaat tcggctgttc
     1441 tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc ggctgaaagc
     1501 gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg
     1561 ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact
     1621 gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac
     1681 tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt
     1741 catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt
     1801 ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa cggtgaacag
     1861 ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa
     1921 cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg
     1981 agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa aaattttgcc
     2041 tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg
     2101 ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc
     2161 tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc
     2221 aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt
     2281 aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta
     2341 ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga
     2401 gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt
     2461 tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa
     2521 ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt
     2581 gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc
     2641 atcagctctc tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc
     2701 atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca
     2761 atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag
     2821 tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc
     2881 aattggtcta ggtgatttta atcactatac caattgagat gggctagtca atgataatta
     2941 ctagtccttt tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa
     3001 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
     3061 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
     3121 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
     3181 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
     3241 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
     3301 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
     3361 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
     3421 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
     3481 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
     3541 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
     3601 caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc
     3661 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     3721 acaggagtcc aagcgagctc tcgaacccca gagtcccgct cagaagaact cgtcaagaag
     3781 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3841 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3901 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3961 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     4021 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     4081 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     4141 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     4201 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     4261 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     4321 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     4381 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     4441 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     4501 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     4561 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     4621 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     4681 tggcaattcc gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4741 tatcacgagg ccctttcgtc ttcacctcga gagtactgcc acggaggctc tagggcggcg
     4801 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     4861 ctttcgactg agcctttcgt tttatttgat gcctctagat taagctgcta aagcgtagtt
     4921 ttcgtcgttt gctgcagacc cactttcaca tttaagttgt ttttctaatc cgcatatgat
     4981 caattcaagg ccgaataaga aggctggctc tgcaccttgg tgatcaaata attcgatagc
     5041 ttgtcgtaat aatggcggca tactatcagt agtaggtgtt tccctttctt ctttagcgac
     5101 ttgatgctct tgatcttcca atacgcaacc taaagtaaaa tgccccacag cgctgagtgc
     5161 atataatgca ttctctagtg aaaaaccttg ttggcataaa aaggctaatt gattttcgag
     5221 agtttcatac tgtttttctg taggccgtgt acctaaatgt acttttgctc catcgcgatg
     5281 acttagtaaa gcacatctaa aacttttagc gttattacgt aaaaaatctt gccagctttc
     5341 cccttctaaa gggcaaaagt gagtatggtg cctatctaac atctcaatgg ctaaggcgtc
     5401 gagcaaagcc cgcttatttt ttacatgcca atacaatgta ggctgctcta cacctagctt
     5461 ctgggcgagt ttacgggttg ttaaaccttc gattccgacc tcattaagca gctctaatgc
     5521 gctgttaatc actttacttt tatctaatct ggacatggta cctttctcct ctttaatgaa
     5581 ttcggtcagt gcgtcctgct gatgtgctca gtatcttgtt atccgctcac aatgtcaatt
     5641 gttatccgct cacaattctc gaggtgaaga cgaaaggagc cattcaggct ctagggcggc
     5701 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     5761 tctttcgact gagcctttcg ttttatttga tgcctctaga tcaagctgct aaagcgtagt
     5821 tttcgtcgtt tgctgcgcca aacgtctctt caggccactg actagcgata actttcccca
     5881 caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     5941 acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     6001 ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     6061 caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     6121 agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     6181 taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     6241 catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     6301 tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca agcaatgcgg
     6361 cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     6421 tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     6481 aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     6541 tgctcatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact cga
//
LOCUS       TCL_RRR                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187736411.162
VERSION     urn.local...1608187736411.162
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     rep_origin      63..2215
                     /label="SC101* Origin (Approx Position)"
     terminator      2258..2363
                     /label="t0"
     CDS             complement(2389..3183)
                     /label="KnR (neo)"
     misc_feature    3395^3396
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(3418..3522)
                     /label="T1"
     CDS             complement(3529..4185)
                     /label="TetR"
     misc_feature    complement(4212..4291)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    4291^4292
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4319..4423)
                     /label="T1"
     CDS             complement(4430..5176)
                     /label="cI"
     misc_feature    complement(5204..5277)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    5282^5283
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5310..5414)
                     /label="T1"
     CDS             complement(5421..6545)
                     /label="LacI"
     misc_feature    complement(6574..6653)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca cctaggcgtc gacagatctc ccgggtaccg agctcgaatt
       61 cggctgttct ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg
      121 gctgaaagcg ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg
      181 gctcccgtgt tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta
      241 tgtgtgactg ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct
      301 ttgttttact ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt
      361 cgatctgttc atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat
      421 ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac
      481 ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag
      541 ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt
      601 ttttgcgtga gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa
      661 aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt
      721 cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc
      781 atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact
      841 tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg
      901 taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca
      961 tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt
     1021 gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag
     1081 tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg
     1141 aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg
     1201 gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca
     1261 gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg
     1321 atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta
     1381 gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc
     1441 tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac
     1501 atacatctca attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa
     1561 tgataattac tagtcctttt cccgggagat ctgggtatct gtaaattctg ctagaccttt
     1621 gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt
     1681 ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata
     1741 aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac
     1801 aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc
     1861 ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat
     1921 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc
     1981 agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc
     2041 cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg
     2101 tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc
     2161 acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg
     2221 tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa
     2281 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2341 gatctatcaa caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc
     2401 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2461 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2521 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     2581 gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc
     2641 gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     2701 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc
     2761 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     2821 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     2881 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     2941 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3001 ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3061 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3121 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3181 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     3241 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     3301 cgccccagct ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa
     3361 aaataggcgt atcacgaggc cctttcgtct tcacctcgag agtactgcca cggaggctct
     3421 agggcggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3481 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctctagatt aagctgctaa
     3541 agcgtagttt tcgtcgtttg ctgcagaccc actttcacat ttaagttgtt tttctaatcc
     3601 gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa
     3661 ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc
     3721 tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccacagc
     3781 gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg
     3841 attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc
     3901 atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg
     3961 ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc
     4021 taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac
     4081 acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag
     4141 ctctaatgcg ctgttaatca ctttactttt atctaatctg gacatggtac ctttctcctc
     4201 tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag tatcttgtta tccgctcaca
     4261 atgtcaattg ttatccgctc acaattctcg aggtgaagac gaaaggagcc attcaggctc
     4321 tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga
     4381 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctctagat caagctgcta
     4441 aagcgtagtt ttcgtcgttt gctgcgccaa acgtctcttc aggccactga ctagcgataa
     4501 ctttccccac aacggaacaa ctctcattgc atgggatcat tgggtactgt gggtttagtg
     4561 gttgtaaaaa cacctgaccg ctatccctga tcagtttctt gaaggtaaac tcatcacccc
     4621 caagtctggc tatgcagaaa tcacctggct caacagcctg ctcagggtca acgagaatta
     4681 acattccgtc aggaaagctt ggcttggagc ctgttggtgc ggtcatggaa ttaccttcaa
     4741 cctcaagcca gaatgcagaa tcactggctt ttttggttgt gcttacccat ctctccgcat
     4801 cacctttggt aaaggttcta agctcaggtg agaacatccc tgcctgaaca tgagaaaaaa
     4861 cagggtactc atactcactt ctaagtgacg gctgcatact aaccgcttca tacatctcgt
     4921 agatttctct ggcgattgaa gggctaaatt cttcaacgct aactttgaga atttttgcaa
     4981 gcaatgcggc gttataagca tttaatgcat tgatgccatt aaataaagca ccaacgcctg
     5041 actgccccat ccccatcttg tctgcgacag attcctggga taagccaagt tcatttttct
     5101 ttttttcata aattgcttta aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt
     5161 tcttttttgt gctcatggta cctttctcct ctttaatgaa ttcggtcagt gcgtcctgct
     5221 gatgtgctca gtatctctat cactgatagg gatgtcaatc tctatcactg atagggactc
     5281 gaggtgaaga cgaaaggagc cagtgaggct ctagggcggc ggatttgtcc tactcaggag
     5341 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     5401 ttttatttga tgcctctaga ttaagctgct aaagcgtagt tttcgtcgtt tgctgcctgc
     5461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5521 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     5581 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5641 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5701 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5761 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5821 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5881 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     5941 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     6001 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     6061 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     6121 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     6181 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     6241 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     6301 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     6361 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     6421 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     6481 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     6541 accatatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatcttgt tatccgctca caatgtcaat tgttatccgc tcacaattct cga
//
LOCUS       TLC_FFF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187746578.205
VERSION     urn.local...1608187746578.205
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     rep_origin      1011..3163
                     /label="SC101* Origin (Approx Position)"
     terminator      3206..3311
                     /label="t0"
     CDS             complement(3337..4131)
                     /label="KnR (neo)"
     misc_feature    4343^4344
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4381^4382
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4382..4461
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4488..5144
                     /label="TetR"
     terminator      5151..5255
                     /label="T1"
     misc_feature    5282^5283
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5283..5362
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             5391..6515
                     /label="LacI"
     terminator      6522..6626
                     /label="T1"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccacc taggcgtcga cagatctccc gggtaccgag ctcgaattcg gctgttctgg
     1021 tgttgctagt ttgttatcag aatcgcagat ccggcttcag gtttgccggc tgaaagcgct
     1081 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     1141 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     1201 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     1261 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     1321 ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     1381 caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg tgaacagttg
     1441 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     1501 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     1561 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     1621 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     1681 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     1741 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     1801 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     1861 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     1921 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     1981 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     2041 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     2101 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     2161 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     2221 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     2281 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     2341 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     2401 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     2461 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     2521 gtccttttcc cgggagatct gggtatctgt aaattctgct agacctttgc tggaaaactt
     2581 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     2641 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     2701 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     2761 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     2821 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     2881 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     2941 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     3001 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     3061 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     3121 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     3181 gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     3241 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3301 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     3361 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3421 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3481 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3541 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3601 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3661 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3721 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3781 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3841 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3901 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3961 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4021 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4081 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4141 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     4201 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4261 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4321 cacgaggccc tttcgtcttc acctcgagag tactgccacg gaggcacctt tcgtcttcac
     4381 ctcgagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
     4441 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg tccagattag
     4501 ataaaagtaa agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt
     4561 taacaacccg taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg
     4621 taaaaaataa gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata
     4681 ctcacttttg ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa
     4741 gttttagatg tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc
     4801 ctacagaaaa acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt
     4861 tttcactaga gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg
     4921 tattggaaga tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata
     4981 gtatgccgcc attattacga caagctatcg aattatttga tcaccaaggt gcagagccag
     5041 ccttcttatt cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa
     5101 gtgggtctgc agcaaacgac gaaaactacg ctttagcagc ttaatctaga ggcatcaaat
     5161 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     5221 cgctctcctg agtaggacaa atccgccgcc ctagagccat tcaggcacct ttcgtcttca
     5281 cctcgagaat tgtgagcgga taacaattga cattgtgagc ggataacaag atactgagca
     5341 catcagcagg acgcactgac cgaattcatt aaagaggaga aaggtaccat atggtgaatg
     5401 tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt
     5461 cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg
     5521 cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt
     5581 cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg
     5641 cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac
     5701 gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg
     5761 ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca
     5821 ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt
     5881 tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc
     5941 aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct
     6001 ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga
     6061 gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg
     6121 cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg
     6181 ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat
     6241 gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg
     6301 tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg
     6361 tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg
     6421 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagg
     6481 cagcaaacga cgaaaactac gctttagcag cttaatctag aggcatcaaa taaaacgaaa
     6541 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     6601 gagtaggaca aatccgccgc cctagagcca gtgaggcacc tttcgtcttc acc
//
LOCUS       TLC_FFR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608500751165.153
VERSION     urn.local...1608500751165.153
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..80
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             109..1233
                     /label="lacI"
     terminator      complement(1247..1351)
                     /label="T1"
     CDS             complement(1358..2104)
                     /label="cI"
     misc_feature    complement(2132..2205)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    2210^2211
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      2273..4425
                     /label="SC101* Origin (Approx Position)"
     terminator      4468..4573
                     /label="t0"
     CDS             complement(4599..5393)
                     /label="KnR (neo)"
     misc_feature    5605^5606
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5643^5644
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    5644..5723
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             5750..6406
                     /label="tetR"
     terminator      6413..6517
                     /label="T1"
ORIGIN      
        1 tcgagaattg tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatat ggtgaatgtg
      121 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
      181 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
      241 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
      301 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
      361 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
      421 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
      481 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
      541 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
      601 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
      661 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
      721 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
      781 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
      841 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
      901 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
      961 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     1021 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     1081 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     1141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcaggca
     1201 gcaaacgacg aaaactacgc tttagcagct taagagccag tgaggctcta gggcggcgga
     1261 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     1321 ttcgactgag cctttcgttt tatttgatgc ctctagatca agctgctaaa gcgtagtttt
     1381 cgtcgtttgc tgcgccaaac gtctcttcag gccactgact agcgataact ttccccacaa
     1441 cggaacaact ctcattgcat gggatcattg ggtactgtgg gtttagtggt tgtaaaaaca
     1501 cctgaccgct atccctgatc agtttcttga aggtaaactc atcaccccca agtctggcta
     1561 tgcagaaatc acctggctca acagcctgct cagggtcaac gagaattaac attccgtcag
     1621 gaaagcttgg cttggagcct gttggtgcgg tcatggaatt accttcaacc tcaagccaga
     1681 atgcagaatc actggctttt ttggttgtgc ttacccatct ctccgcatca cctttggtaa
     1741 aggttctaag ctcaggtgag aacatccctg cctgaacatg agaaaaaaca gggtactcat
     1801 actcacttct aagtgacggc tgcatactaa ccgcttcata catctcgtag atttctctgg
     1861 cgattgaagg gctaaattct tcaacgctaa ctttgagaat ttttgcaagc aatgcggcgt
     1921 tataagcatt taatgcattg atgccattaa ataaagcacc aacgcctgac tgccccatcc
     1981 ccatcttgtc tgcgacagat tcctgggata agccaagttc atttttcttt ttttcataaa
     2041 ttgctttaag gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc ttttttgtgc
     2101 tcatggtacc tttctcctct ttaatgaatt cggtcagtgc gtcctgctga tgtgctcagt
     2161 atctctatca ctgataggga tgtcaatctc tatcactgat agggactcga ggtgaagacg
     2221 aaaggagcca cctaggcgtc gacagatctc ccgggtaccg agctcgaatt cggctgttct
     2281 ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg gctgaaagcg
     2341 ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt
     2401 tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg
     2461 ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact
     2521 ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc
     2581 atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt
     2641 tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac ggtgaacagt
     2701 tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac
     2761 ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga
     2821 gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa aattttgcct
     2881 caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt
     2941 tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct
     3001 ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca
     3061 acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta
     3121 aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat
     3181 tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag
     3241 ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt
     3301 caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag
     3361 gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg
     3421 tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca
     3481 tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca
     3541 tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa
     3601 tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt
     3661 catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca
     3721 attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac
     3781 tagtcctttt cccgggagat ctgggtatct gtaaattctg ctagaccttt gctggaaaac
     3841 ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt
     3901 atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag
     3961 atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt
     4021 cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca
     4081 ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     4141 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     4201 ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa
     4261 gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc
     4321 tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt
     4381 atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac
     4441 aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg
     4501 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     4561 caggagtcca agcgagctct cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     4621 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4681 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4741 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4801 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     4861 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     4921 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     4981 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     5041 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     5101 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     5161 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     5221 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     5281 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     5341 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     5401 gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc
     5461 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     5521 ggcaattccg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     5581 atcacgaggc cctttcgtct tcacctcgag agtactgcca cggaggcacc tttcgtcttc
     5641 acctcgagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa gatactgagc
     5701 acatcagcag gacgcactga ccgaattcat taaagaggag aaaggtacca tgtccagatt
     5761 agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg
     5821 tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca
     5881 tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca
     5941 tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa
     6001 aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg
     6061 gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg
     6121 tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg
     6181 cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga
     6241 tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc
     6301 agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga
     6361 aagtgggtct gcagcaaacg acgaaaacta cgctttagca gcttaatcta gaggcatcaa
     6421 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     6481 aacgctctcc tgagtaggac aaatccgccg ccctagagcc attcaggcac ctttcgtctt
     6541 cacc
//
LOCUS       TLC_FRF                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187746558.203
VERSION     urn.local...1608187746558.203
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    43^44
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    49..122
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             150..896
                     /label="cI"
     terminator      903..1007
                     /label="T1"
     rep_origin      1054..3206
                     /label="SC101* Origin (Approx Position)"
     terminator      3249..3354
                     /label="t0"
     CDS             complement(3380..4174)
                     /label="KnR (neo)"
     misc_feature    4386^4387
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4424^4425
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4425..4504
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4531..5187
                     /label="TetR"
     terminator      5194..5298
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="LacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc acctcgagtc cctatcagtg
       61 atagagattg acatccctat cagtgataga gatactgagc acatcagcag gacgcactga
      121 ccgaattcat taaagaggag aaaggtacca tgagcacaaa aaagaaacca ttaacacaag
      181 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg
      241 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt
      301 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca
      361 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag
      421 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc
      481 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg
      541 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca
      601 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc
      661 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta
      721 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt
      781 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt
      841 ggcctgaaga gacgtttggc gcagcaaacg acgaaaacta cgctttagca gcttgatcta
      901 gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      961 tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ccctagagcc acctaggcgt
     1021 cgacagatct cccgggtacc gagctcgaat tcggctgttc tggtgttgct agtttgttat
     1081 cagaatcgca gatccggctt caggtttgcc ggctgaaagc gctatttctt ccagaattgc
     1141 catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg
     1201 ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt
     1261 ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta
     1321 ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttaaat
     1381 gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg
     1441 catatggaca gttttccctt tgatatctaa cggtgaacag ttgttctact tttgtttgtt
     1501 agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt
     1561 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt
     1621 gagatcatgc ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat
     1681 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga
     1741 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt
     1801 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc
     1861 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt
     1921 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa
     1981 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc
     2041 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc
     2101 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa
     2161 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa
     2221 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt
     2281 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata
     2341 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc
     2401 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag
     2461 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta
     2521 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcccgggaga
     2581 tctgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc
     2641 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat
     2701 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa
     2761 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
     2821 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
     2881 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
     2941 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
     3001 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
     3061 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
     3121 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
     3181 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
     3241 tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3301 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     3361 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     3421 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     3481 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     3541 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     3601 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     3661 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     3721 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     3781 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     3841 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     3901 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     3961 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     4021 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     4081 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     4141 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     4201 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     4261 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc gacgtctaag
     4321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4381 ttcacctcga gagtactgcc acggaggcac ctttcgtctt cacctcgaga attgtgagcg
     4441 gataacaatt gacattgtga gcggataaca agatactgag cacatcagca ggacgcactg
     4501 accgaattca ttaaagagga gaaaggtacc atgtccagat tagataaaag taaagtgatt
     4561 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     4621 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     4681 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     4741 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     4801 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     4861 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     4921 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     4981 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     5041 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     5101 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc tgcagcaaac
     5161 gacgaaaact acgctttagc agcttaatct agaggcatca aataaaacga aaggctcagt
     5221 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     5281 caaatccgcc gccctagagc cattcaggct cttaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       TLC_FRR                 6544 bp    DNA     circular UNA 20-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608501144877.263
VERSION     urn.local...1608501144877.263
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..885)
                     /label="cI"
     misc_feature    complement(913..986)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      1054..3206
                     /label="SC101* Origin (Approx Position)"
     terminator      3249..3354
                     /label="t0"
     CDS             complement(3380..4174)
                     /label="KnR (neo)"
     misc_feature    4386^4387
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4424^4425
                     /note="Geneious type: origin"
                     /label="origin"
     misc_feature    4425..4504
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     CDS             4531..5187
                     /label="tetR"
     terminator      5194..5298
                     /label="T1"
     CDS             complement(5312..6436)
                     /label="lacI"
     misc_feature    complement(6465..6544)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatc aagctgctaa agcgtagttt tcgtcgtttg ctgcgccaaa
      181 cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca
      241 tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat
      301 cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc
      361 aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc
      421 tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt
      481 tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga
      541 gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg
      601 ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc
      661 ttcaacgcta actttgagaa tttttgcaag caatgcggcg ttataagcat ttaatgcatt
      721 gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga
      781 ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc
      841 gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatggtac ctttctcctc
      901 tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag tatctctatc actgataggg
      961 atgtcaatct ctatcactga tagggactcg aggtgaagac gaaaggagcc acctaggcgt
     1021 cgacagatct cccgggtacc gagctcgaat tcggctgttc tggtgttgct agtttgttat
     1081 cagaatcgca gatccggctt caggtttgcc ggctgaaagc gctatttctt ccagaattgc
     1141 catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg
     1201 ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt
     1261 ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta
     1321 ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttaaat
     1381 gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg
     1441 catatggaca gttttccctt tgatatctaa cggtgaacag ttgttctact tttgtttgtt
     1501 agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt
     1561 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt
     1621 gagatcatgc ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat
     1681 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga
     1741 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt
     1801 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc
     1861 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt
     1921 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa
     1981 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc
     2041 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc
     2101 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa
     2161 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa
     2221 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt
     2281 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata
     2341 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc
     2401 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag
     2461 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta
     2521 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcccgggaga
     2581 tctgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc
     2641 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat
     2701 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa
     2761 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
     2821 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
     2881 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
     2941 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
     3001 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
     3061 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
     3121 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
     3181 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
     3241 tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3301 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     3361 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     3421 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     3481 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     3541 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     3601 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     3661 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     3721 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     3781 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     3841 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     3901 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     3961 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     4021 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     4081 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     4141 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     4201 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     4261 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc gacgtctaag
     4321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4381 ttcacctcga gagtactgcc acggaggcac ctttcgtctt cacctcgaga attgtgagcg
     4441 gataacaatt gacattgtga gcggataaca agatactgag cacatcagca ggacgcactg
     4501 accgaattca ttaaagagga gaaaggtacc atgtccagat tagataaaag taaagtgatt
     4561 aacagcgcat tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc
     4621 gcccagaagc taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct
     4681 ttgctcgacg ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta
     4741 gaaggggaaa gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta
     4801 ctaagtcatc gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat
     4861 gaaactctcg aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca
     4921 ttatatgcac tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag
     4981 catcaagtcg ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta
     5041 cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt
     5101 gaattgatca tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc tgcagcaaac
     5161 gacgaaaact acgctttagc agcttaatct agaggcatca aataaaacga aaggctcagt
     5221 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     5281 caaatccgcc gccctagagc cattcaggct cttaagctgc taaagcgtag ttttcgtcgt
     5341 ttgctgcctg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
     5461 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
     5521 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
     5581 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
     5641 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
     5701 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
     5761 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
     5821 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
     5881 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
     5941 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
     6001 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
     6061 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
     6121 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
     6181 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
     6241 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
     6301 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
     6361 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
     6421 gtttcacatt caccatatgg tacctttctc ctctttaatg aattcggtca gtgcgtcctg
     6481 ctgatgtgct cagtatcttg ttatccgctc acaatgtcaa ttgttatccg ctcacaattc
     6541 tcga
//
LOCUS       TLC_RRF                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187746548.199
VERSION     urn.local...1608187746548.199
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    6..79
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     CDS             107..853
                     /label="cI"
     terminator      860..964
                     /label="T1"
     rep_origin      1011..3163
                     /label="SC101* Origin (Approx Position)"
     terminator      3206..3311
                     /label="t0"
     CDS             complement(3337..4131)
                     /label="KnR (neo)"
     misc_feature    4343^4344
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4366..4470)
                     /label="T1"
     CDS             complement(4477..5133)
                     /label="TetR"
     misc_feature    complement(5160..5239)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5239^5240
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5267..5371)
                     /label="T1"
     CDS             complement(5378..6502)
                     /label="LacI"
     misc_feature    complement(6531..6610)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    6610^6611
                     /note="Geneious type: origin"
                     /label="origin"
ORIGIN      
        1 tcgagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcaca
       61 tcagcaggac gcactgaccg aattcattaa agaggagaaa ggtaccatga gcacaaaaaa
      121 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      181 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      241 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      301 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      361 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      421 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      481 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      541 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      601 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      661 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      721 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      781 agttatcgct agtcagtggc ctgaagagac gtttggcgca gcaaacgacg aaaactacgc
      841 tttagcagct tgatctagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc
      961 tagagccacc taggcgtcga cagatctccc gggtaccgag ctcgaattcg gctgttctgg
     1021 tgttgctagt ttgttatcag aatcgcagat ccggcttcag gtttgccggc tgaaagcgct
     1081 atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg
     1141 tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt
     1201 gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg
     1261 tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat
     1321 ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta
     1381 caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg tgaacagttg
     1441 ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct
     1501 cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc
     1561 catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa ttttgcctca
     1621 aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta
     1681 cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg
     1741 ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac
     1801 gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa
     1861 tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt
     1921 tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt
     1981 ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca
     2041 aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa aagataaggc
     2101 aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc
     2161 cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc
     2221 agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc
     2281 tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc
     2341 gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca
     2401 tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat
     2461 tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta
     2521 gtccttttcc cgggagatct gggtatctgt aaattctgct agacctttgc tggaaaactt
     2581 gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat
     2641 attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat
     2701 cccagccctg tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg
     2761 caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc
     2821 ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag
     2881 tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg
     2941 taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga
     3001 aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg
     3061 actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat
     3121 cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag
     3181 gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac gcccggcggc
     3241 aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca
     3301 ggagtccaag cgagctctcg aaccccagag tcccgctcag aagaactcgt caagaaggcg
     3361 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
     3421 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
     3481 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
     3541 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     3601 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     3661 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     3721 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     3781 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     3841 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     3901 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     3961 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     4021 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     4081 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga
     4141 tcctcatcct gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa
     4201 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
     4261 caattccgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4321 cacgaggccc tttcgtcttc acctcgagag tactgccacg gaggctctag ggcggcggat
     4381 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4441 tcgactgagc ctttcgtttt atttgatgcc tctagattaa gctgctaaag cgtagttttc
     4501 gtcgtttgct gcagacccac tttcacattt aagttgtttt tctaatccgc atatgatcaa
     4561 ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt cgatagcttg
     4621 tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt tagcgacttg
     4681 atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc tgagtgcata
     4741 taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat tttcgagagt
     4801 ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat cgcgatgact
     4861 tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc agctttcccc
     4921 ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta aggcgtcgag
     4981 caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac ctagcttctg
     5041 ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct ctaatgcgct
     5101 gttaatcact ttacttttat ctaatctgga catggtacct ttctcctctt taatgaattc
     5161 ggtcagtgcg tcctgctgat gtgctcagta tcttgttatc cgctcacaat gtcaattgtt
     5221 atccgctcac aattctcgag gtgaagacga aaggagccat tcaggctcta gggcggcgga
     5281 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct
     5341 ttcgactgag cctttcgttt tatttgatgc ctctagatta agctgctaaa gcgtagtttt
     5401 cgtcgtttgc tgcctgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5461 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     5521 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     5581 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     5641 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     5701 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     5761 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     5821 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     5881 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     5941 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     6001 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     6061 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     6121 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     6181 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     6241 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     6301 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     6361 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     6421 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     6481 ttactggttt cacattcacc atatggtacc tttctcctct ttaatgaatt cggtcagtgc
     6541 gtcctgctga tgtgctcagt atcttgttat ccgctcacaa tgtcaattgt tatccgctca
     6601 caattctcga ggtgaagacg aaaggagcca gtgaggcacc tttcgtcttc acc
//
LOCUS       TLC_RRR                 6653 bp    DNA     circular UNA 17-DEC-2020
DEFINITION  Concatenation of 4 sequences.
ACCESSION   urn.local...1608187746538.198
VERSION     urn.local...1608187746538.198
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(28..132)
                     /label="T1"
     CDS             complement(139..885)
                     /label="cI"
     misc_feature    complement(913..986)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PtetO1"
     misc_feature    991^992
                     /note="Geneious type: origin"
                     /label="origin"
     rep_origin      1054..3206
                     /label="SC101* Origin (Approx Position)"
     terminator      3249..3354
                     /label="t0"
     CDS             complement(3380..4174)
                     /label="KnR (neo)"
     misc_feature    4386^4387
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(4409..4513)
                     /label="T1"
     CDS             complement(4520..5176)
                     /label="TetR"
     misc_feature    complement(5203..5282)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
     misc_feature    5282^5283
                     /note="Geneious type: origin"
                     /label="origin"
     terminator      complement(5310..5414)
                     /label="T1"
     CDS             complement(5421..6545)
                     /label="LacI"
     misc_feature    complement(6574..6653)
                     /note="Geneious type: promoter prokaryotic"
                     /label="PLlacO1"
ORIGIN      
        1 ggtgaagacg aaaggagcca gtgaggctct agggcggcgg atttgtccta ctcaggagag
       61 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
      121 ttatttgatg cctctagatc aagctgctaa agcgtagttt tcgtcgtttg ctgcgccaaa
      181 cgtctcttca ggccactgac tagcgataac tttccccaca acggaacaac tctcattgca
      241 tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac acctgaccgc tatccctgat
      301 cagtttcttg aaggtaaact catcaccccc aagtctggct atgcagaaat cacctggctc
      361 aacagcctgc tcagggtcaa cgagaattaa cattccgtca ggaaagcttg gcttggagcc
      421 tgttggtgcg gtcatggaat taccttcaac ctcaagccag aatgcagaat cactggcttt
      481 tttggttgtg cttacccatc tctccgcatc acctttggta aaggttctaa gctcaggtga
      541 gaacatccct gcctgaacat gagaaaaaac agggtactca tactcacttc taagtgacgg
      601 ctgcatacta accgcttcat acatctcgta gatttctctg gcgattgaag ggctaaattc
      661 ttcaacgcta actttgagaa tttttgcaag caatgcggcg ttataagcat ttaatgcatt
      721 gatgccatta aataaagcac caacgcctga ctgccccatc cccatcttgt ctgcgacaga
      781 ttcctgggat aagccaagtt catttttctt tttttcataa attgctttaa ggcgacgtgc
      841 gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg ctcatggtac ctttctcctc
      901 tttaatgaat tcggtcagtg cgtcctgctg atgtgctcag tatctctatc actgataggg
      961 atgtcaatct ctatcactga tagggactcg aggtgaagac gaaaggagcc acctaggcgt
     1021 cgacagatct cccgggtacc gagctcgaat tcggctgttc tggtgttgct agtttgttat
     1081 cagaatcgca gatccggctt caggtttgcc ggctgaaagc gctatttctt ccagaattgc
     1141 catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag ctttgattcg
     1201 ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt aacaagttgt
     1261 ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc tgttctatta
     1321 ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac agctttaaat
     1381 gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt ttcatctgtg
     1441 catatggaca gttttccctt tgatatctaa cggtgaacag ttgttctact tttgtttgtt
     1501 agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc ttccgtattt
     1561 agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga acgaaccatt
     1621 gagatcatgc ttactttgca tgtcactcaa aaattttgcc tcaaaactgg tgagctgaat
     1681 ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttacgtaggt aggaatctga
     1741 tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct caagttcggt
     1801 tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag tcgggcggcc
     1861 tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac ttattggttt
     1921 caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca ttaacatgaa
     1981 cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt gtgttagttc
     2041 ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact taacatgttc
     2101 cagattatat tttatgaatt tttttaactg gaaaagataa ggcaatatct cttcactaaa
     2161 aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga aaatctcaaa
     2221 gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc tggttgcttt
     2281 agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt attggttata
     2341 agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt tgagtagtgc
     2401 cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac taatcgctag
     2461 ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta ggtgatttta
     2521 atcactatac caattgagat gggctagtca atgataatta ctagtccttt tcccgggaga
     2581 tctgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt ctgctagacc
     2641 ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag tggttataat
     2701 ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc ctgtgtataa
     2761 ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc tgtttgctcc
     2821 tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa gctcgggcaa
     2881 atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt ctttttcgtg
     2941 acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg cactacaggc
     3001 gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc gtcacgggct
     3061 tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt gctgttcagc
     3121 agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga caggtcattc
     3181 agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc cgtcttactg
     3241 tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac
     3301 aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc
     3361 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
     3421 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
     3481 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
     3541 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
     3601 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
     3661 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
     3721 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
     3781 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
     3841 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
     3901 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
     3961 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
     4021 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
     4081 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
     4141 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
     4201 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
     4261 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc gacgtctaag
     4321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     4381 ttcacctcga gagtactgcc acggaggctc tagggcggcg gatttgtcct actcaggaga
     4441 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
     4501 tttatttgat gcctctagat taagctgcta aagcgtagtt ttcgtcgttt gctgcagacc
     4561 cactttcaca tttaagttgt ttttctaatc cgcatatgat caattcaagg ccgaataaga
     4621 aggctggctc tgcaccttgg tgatcaaata attcgatagc ttgtcgtaat aatggcggca
     4681 tactatcagt agtaggtgtt tccctttctt ctttagcgac ttgatgctct tgatcttcca
     4741 atacgcaacc taaagtaaaa tgccccacag cgctgagtgc atataatgca ttctctagtg
     4801 aaaaaccttg ttggcataaa aaggctaatt gattttcgag agtttcatac tgtttttctg
     4861 taggccgtgt acctaaatgt acttttgctc catcgcgatg acttagtaaa gcacatctaa
     4921 aacttttagc gttattacgt aaaaaatctt gccagctttc cccttctaaa gggcaaaagt
     4981 gagtatggtg cctatctaac atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt
     5041 ttacatgcca atacaatgta ggctgctcta cacctagctt ctgggcgagt ttacgggttg
     5101 ttaaaccttc gattccgacc tcattaagca gctctaatgc gctgttaatc actttacttt
     5161 tatctaatct ggacatggta cctttctcct ctttaatgaa ttcggtcagt gcgtcctgct
     5221 gatgtgctca gtatcttgtt atccgctcac aatgtcaatt gttatccgct cacaattctc
     5281 gaggtgaaga cgaaaggagc cattcaggct ctagggcggc ggatttgtcc tactcaggag
     5341 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
     5401 ttttatttga tgcctctaga ttaagctgct aaagcgtagt tttcgtcgtt tgctgcctgc
     5461 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5521 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     5581 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5641 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5701 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5761 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5821 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5881 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     5941 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     6001 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     6061 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     6121 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     6181 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     6241 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     6301 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     6361 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     6421 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     6481 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     6541 accatatggt acctttctcc tctttaatga attcggtcag tgcgtcctgc tgatgtgctc
     6601 agtatcttgt tatccgctca caatgtcaat tgttatccgc tcacaattct cga
//
